scholarly journals Damage to the Testicular Structure of Rats by Acute Oral Exposure of Cadmium

2021 ◽  
Vol 18 (11) ◽  
pp. 6038
Author(s):  
Tariq Iqbal ◽  
Maosheng Cao ◽  
Zijiao Zhao ◽  
Yun Zhao ◽  
Lu Chen ◽  
...  
Keyword(s):  
Weight Gain ◽  
Reproductive System ◽  
Sperm Count ◽  
Tunica Albuginea ◽  
Tail Length ◽  
Oral Exposure ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Sprague Dawley ◽  
Significant Difference

Cadmium (Cd) is one of the most important heavy metal toxicants, used throughout the world at the industrial level. It affects humans through environmental and occupational exposure and animals through the environment. The most severe effects of oral exposure to Cd on the male reproductive system, particularly spermatogenesis, have not been discussed. In this study, we observed the damage to the testes and heritable DNA caused by oral exposure to Cd. Adult male Sprague–Dawley rats were divided into four groups: a control group and three groups treated with 5, 10, and 15 mg Cd/kg/day for 17 days by oral gavage. Our results revealed that Cd significantly decreases weight gain in 10 and 15 mg/kg groups, whereas the 5 mg/kg groups showed no difference in weight gain. The histopathology showed adverse structural effects on the rat testis by significantly reducing the thickness of the tunica albuginea, the diameter of the tubular lumen, and the interstitial space among seminiferous tubules and increasing the height of the epithelium and the diameter of the seminiferous tubules in Cd treated groups. Comet assay in epididymal sperms demonstrated a significant difference in the lengths of the head and comet in all the 3 Cd treated groups, indicating damage in heritable DNA, although variations in daily sperm production were not significant. Only a slight decrease in sperm count was reported in Cd-treated groups as compared to the control group, whereas the tail length, percentage of DNA in head, and tail showed no significant difference in control and all the experimental groups. Overall, our findings indicate that Cd toxicity must be controlled using natural sources, such as herbal medicine or bioremediation, with non-edible plants, because it could considerably affect heritable DNA and induce damage to the reproductive system.

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

Evaluation of Reproductive Development Following Intravenous and Oral Exposure to DEHP in Male Neonatal Rats

2003 ◽  
Vol 22 (3) ◽  
pp. 159-174 ◽  
Author(s):  
Jon N. Cammack ◽  
Randy D. White ◽  
Donovan Gordon ◽  
Jerome Gass ◽  
Lawrence Hecker ◽  
...  
Keyword(s):  
Sperm Count ◽  
Liver Weight ◽  
Oral Exposure ◽  
Seminiferous Tubules ◽  
Primary Group ◽  
Sprague Dawley ◽  
Intravenous Injections ◽  
Sprague Dawley Rats ◽  
Recovery Group ◽  
Ethylhexyl Phthalate

Di-(2-ethylhexyl)phthalate (DEHP) was administered to 3- to 5-day-old male Sprague-Dawley rats by daily intravenous injections of 60, 300, or 600 mg/kg/day or by daily oral gavage of 300 or 600 mg/kg/day for 21 days. Histopathological evaluation and organ weight measurements were performed on some animals after 21 days of dosing (primary group) and later on the recovery group animals that were held without further treatment until sexual maturity at approximately 90 days of age. No effects of any type were observed in animals treated intravenously with 60 mg/kg/day. Testicular changes, consisting of a partial depletion of the germinal epithelium and/or decrease in diameter of seminiferous tubules, were present in all animals of the 300- and 600-mg/kg/day groups after the 21-day dosing period. Testes weight decreased and liver weight increased in these animals. Testes changes were dose-related and generally more severe among animals dosed orally versus intravenously. In the recovery animals, a residual DEHP-induced decrease in seminiferous tubule diameter was present in the testis of several animals dosed orally at 300 and 600 mg/kg/day, but not in animals dosed intravenously. There was no germinal cell depletion or Sertoli cell alteration observed in any dose group at any time. Notably, no effects on sperm count, sperm morphology, or sperm motility were observed at 90 days of age in any of the groups.

Two-Generation Oral (Diet) Reproductive Toxicity Study of Resorcinol Bis-Diphenylphosphate (Fyrolflex RDP) in Rats

2000 ◽  
Vol 19 (4) ◽  
pp. 243-255 ◽  
Author(s):  
R. Henrich ◽  
B. M. Ryan ◽  
R. Selby ◽  
S. Garthwaite ◽  
R. Morrissey ◽  
...  
Keyword(s):  
Body Weight ◽  
Sperm Count ◽  
Clinical Signs ◽  
Control Group ◽  
High Dose ◽  
Test Substance ◽  
Sprague Dawley ◽  
Mating Period ◽  
Flavor Aversion ◽  
Significant Difference

Fyrolflex resorcinol bis-diphenylphosphate (RDP) was evaluated in a two-generation reproductive study as part of a program to assess the overall toxicology of this flame retardant. RDP was administered to male and female Sprague-Dawley rats in the diet at concentrations of 1000, 10,000 or 20,000 ppm. The control group was given diet alone. Parental (P1) animals were treated for 10 weeks prior to mating, during the 2-week mating period, throughout gestation, and through lactation until sacrifice. The F1 generation (P1 offspring) was treated following a regimen similar to P1. The F2 generation was not treated. No significant difference in Utter survival was observed between the control and treated groups. Body weights were significantly decreased in P1 rats during the 1st week due to an initial flavor aversion of the test substance in the diet. Body weight, weight gains, and food consumption were decreased in the test substance-treated pups (F1) during lactationand after weaning. These changes were also attributed to a flavor aversion. Anogenital distance was similar in the control and high-dose groups, whereas vaginal opening and preputial separation were delayed in the 10,000 and 20,000 ppm groups, and were considered to be secondary to the reduction in F1 body weight. Neither parents nor offspring exhibited any test substance-related clinical signs of toxicity. Vaginal cytology and cyclicity and male reproductive functions (sperm count, motility, and morphology) were unaffected by treatment. Mating performance was similar in the treated groups relative to the control. No treatment-related lesions were noted in the reproductive organs. Increased liver weight and associated hepatic periportal hypertrophy were observed in the RDP-treated animals (P1 and F1). In conclusion, there were no adverse effects on reproductive performance or fertility parameters associated with RDP administration in the diet. Fyrolflex RDP administered for greater than 13 weeks and up to the entire life span (i.e., F1, from conception to euthanasia) resulted in increased liver weights with associated periportal hypertrophy. This change was considered an adaptive process associated with RDP metabolism in the liver.

The Impact of Gemcitabine-induced Reproductive Toxicity on The Sperm count and Morphology of Albino Rats

2021 ◽  
Vol 12 (1) ◽  
pp. 357-365
Author(s):  
Bassam Abdulaziz Alahmadi
Keyword(s):  
Reproductive System ◽  
Sperm Count ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Human Dose ◽  
Significant Difference ◽  
Recovery Group ◽  
Sperm Count And Morphology ◽  
The Impact

Cancer treatments can affect sperm production and a significant percentage of cancer patients may develop permanent azoospermia or severe oligozoospermia after chemotherapy. To investigate the influence of Gemcitabine toxicity on the reproductive system of albino male rats (sperm count and morphology). An experimental animal study conducted in the zoology department, College of Science, King Saud University during the period from June to October 2014 using albino rats (Rattusnorvegicus) (Wistar strain). Males were divided into four different groups (control" 0 mg/kg",7 mg/kg,14 mg/kg, and 21 mg/kg). The reproductive organs, testicles and epididymis decreased in weight and atrophied in most of the animals treated with the drug in various doses. The mean absolute and relative epididymal weights were also significantly decreased. In the drug-effects recovery group, neither the testicles nor the epididymis in the animals treated with the three doses recovered fully normal weight. The testis's efficiency in producing sperm was significantly decreased at all doses. In the recovery group, the testis regained its efficiency, as no significant difference was recorded between the drug-treated groups and the control group. The drug caused complete loss of sperm, in a rat treated with the big dose. Gemcitabine caused a significant increase in the percentage of deformed sperms in all treated animals. Gemcitabine drug has high toxicity on the reproductive system of rats with a dose tenth of human dose, with a massive decrease in the count and quantity of sperm, which means that this drug can have more toxicity effects on human.

Impact of supplementing commercial rabbit pellets with fresh Moringa oleifera leaves on semen characteristics in rabbit bucks under tropical climate in Trinidad

2021 ◽  
Author(s):  
Krishna Mohan Kumar
Keyword(s):  
Sperm Motility ◽  
Moringa Oleifera ◽  
Sperm Count ◽  
Control Group ◽  
Computer Assisted ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Moringa Oleifera Leaves ◽  
The Impact

Objective This study aimed to evaluate the impact of the dietary supplement of Moringa oleifera leaves (MOL) on semen quality and characteristics in rabbits. Methods Eighteen (n=18) breeding bucks of New Zealand white, of similar age group, were used for the study. Three feeding regimes, (i) 100% commercial rabbit pellets (CRP)-Group I (ii) 90% CRP + 10% fresh MOL on a dry matter (DM) basis – Group II and (iii) 80% CRP + 20% fresh MOL on a DM basis – Group III, were adopted and the trial continued for 21 days. After adaptation to the diet, semen was collected from each buck and subjected to evaluation using a computer-assisted semen analyser. Results In Group III, the sperm count, normal sperm morphology, and sperm motility increased (52.0%) in comparison with the control (Group I; 50.1%). The inclusion of 20% Moringa oliefera in the diet (Group III) caused a significant increase (P<0.05) in semen concentration (Control =136.2 M/mL; Group III=297.2 M/mL). There was no significant difference (P>0.05) in sperm motility and semen volume among the groups. Conclusion The results suggest that supplementing commercial rabbit pellets with 20% fresh Moringa oliefera leaves on a DM basis can improve the quality and characteristics of semen in breeding bucks.

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

Effects of Microwave Oven Exposed Diet on Animal Weight and Testicular Tissue and Relative Role of Mentha Piperita

2021 ◽  
Vol 15 (9) ◽  
pp. 2352-2354
Author(s):  
Kishwar Naheed ◽  
Humaira Ali ◽  
Fareeha Mushtaq ◽  
Muhammad Saad Abdullah ◽  
Maria Yousaf ◽  
...  
Keyword(s):  
Weight Gain ◽  
Testicular Tissue ◽  
Microwave Oven ◽  
Mentha Piperita ◽  
Control Group ◽  
Testis Weight ◽  
Relative Role ◽  
Protective Effects ◽  
Trial Methodology ◽  
Significant Difference

Background: Usage of electronic gadgets like microwave oven is increasing day by day that heats the food by exposing it to electromagnetic radiations which has many hazardous effects on human health including fertility. Aim: To find the effects of microwave oven exposed diet on weight and testis of mice along with protective effects of Mentha Piperita and Melatonin Study Design: Randomized control trial. Methodology: Adult male mice (n=32) were divided into four groups. Control group (G1) received standard pellets prepared for mice. Second group (G2) was given mice pellets exposed to microwave oven. Third group (G3) received Mentha Piperita leaf extract along with mice pellets exposed to microwave oven and the fourth group (G4) received oral melatonin along with pellets exposed to microwave oven. After experimental period, wt of each mice was again recorded and then mice were sacrificed. Data analyzed by SPSS 21.0v. Results: There was no statistically significant difference of weight gain of animals but there was significant reduction in weight of testis in group G2 but in G3 and G4 wt of the testis was close to control. Conclusion: It was concluded that microwave oven exposed diet had no significant effect on overall weight gain of the animal but it significantly reduced weight of the testis in group G2. However, Mentha Piperita and Melatonin both had ameliorative effects on the wt of the testicular tissue. Keywords: Mice, Testis, Weight, Mentha piperita and Melatonin

scholarly journals Effect of piglet castration with nonsurgical sterilant on the zootechnical performance and pork carcass quality

2016 ◽  
Vol 68 (6) ◽  
pp. 1487-1496 ◽  
Author(s):  
D.S. Lucas ◽  
E.T.F. Siqueira ◽  
M.M.H. Haguiwara ◽  
S.S. Azevedo ◽  
S.E. Yotsuyanagi ◽  
...  
Keyword(s):  
Weight Gain ◽  
Meat Quality ◽  
Treated Group ◽  
Control Group ◽  
Active Principle ◽  
Carcass Quality ◽  
Feed Conversion ◽  
Zinc Gluconate ◽  
Hormone Levels ◽  
Significant Difference

ABSTRACT A non-surgical sterilant (NSS) was used with the objective of assessing the zootechnical performance, carcass and meat quality, and hormone levels. 90 male piglets were selected with birthweights varying between 1.5kg to 2.0kg. The control group was constituted of 45 males castrated by the conventional surgical method, at the seventh day of age, and the treated group was constituted of 45 males castrated with NSS (active principle of zinc gluconate in the concentration of 26.2mg/mL associated to the dimethyl sulfoxide at 0.5%), with the application of the first dose on the seventh day of age and the second dose on the fourteenth day of age. The zootechnical assessments were carried out on the farm and consisted: weight gain in the periods per animal and feed conversion. The slaughter of the animals and the assessment of the carcass and meat quality and hormone levels were carried out in a slaughterhouse and the municipality of Campinas (SP). The study demonstrated that the use of NSS was a viable alternative in relation to the zootechnical performance and the carcass quality. In relation to the meat quality there was no significant difference in the majority of the assessed parameters.

scholarly journals Evaluation of Growth and Carcass Characteristics of Broiler Chickens (Cobb 500) Feed on Different Level of Organic Acids Inclusion in Diet at Parwanipur

2019 ◽  
Vol 36 ◽  
pp. 137-147
Author(s):  
A. K. Jha ◽  
Md. H. Azad ◽  
Md.S. N. Ali ◽  
P. Alam ◽  
N. Sheikh ◽  
...  
Keyword(s):  
Weight Gain ◽  
Organic Acids ◽  
Formic Acid ◽  
Broiler Chickens ◽  
Agricultural Research ◽  
Body Weight Gain ◽  
Control Group ◽  
Research Station ◽  
Body Parts ◽  
Significant Difference

 An experiment was carried out to evaluate the effect of different level of organic acids on productive traits, carcass yields and body parts (Thigh, Back, Neck, wings and Breast) of broiler chicken. This experiment was conducted at Regional Agricultural Research Station, Parwanipur, Bara for 41 days to test the effect of organic acids inclusion in broiler feed and its effect on growth performance and carcass study. Altogether 225 day old Cobb-500 broiler birds were procured from Shivam Hatchery, Birgung and divided into 5 treatments with 3 replications (15 birds in each replication) by using completely randomized design. Concentrate feed was purchased from Posak Feed industry, Birgung. Control group (T1) was feed without organic acid inclusion and whereas T2, T3, T4 and T5 groups were fed concentrate mixture mixed with different combination of organic acids @ 400ml/ per 100 kg feed, respectively. Experimental birds were provided adlib amount of grower feed (B1) for 21 days and that after finisher feed (B3) for 20 days and had easily access to drinking water. Feed intake was recorded daily and body weight gain was measured in 7 days interval. Experiment revealed that highest weight gain was observed in T2 (2.6 kg) followed by T3 (2.5) where combination of organic acids were formic acid 65% + propionic acid 35% and formic acid 65% and citric acid 35%, respectively, however, it was not significant among diet groups. From every treatment group each birds were selected for carcass and body parts study. The study showed significant difference (P<0.5) in carcass quantity and body parts of the birds between the treatment groups. The dressing percentage of T1, T2, T3, T4 and T5 were 68.96%, 67.87%, 70.38%, 69.88% and 69.67% respectively. Experiment suggested that further study should be carried out to precise the appropriate level of organic acids inclusion and higher cost benefit ratio.

scholarly journals Shengjing Capsule Improves Spermatogenesis through Upregulating Integrin α6/β1 in the NOA Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Jiamin Wang ◽  
Shankun Zhao ◽  
Lianmin Luo ◽  
Yangzhou Liu ◽  
Ermao Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Sperm Quality ◽  
Sperm Count ◽  
Spermatogonial Stem Cells ◽  
Normal Group ◽  
Seminiferous Tubules ◽  
High Dose ◽  
Sprague Dawley ◽  
Underlying Mechanisms ◽  
Almost All

Objective. To evaluate the therapeutic effect of Shengjing capsules on nonobstructive azoospermia (NOA) in the rat model. Methods. Twenty-five male Sprague–Dawley rats were randomly divided into five groups as follows (n=5 per group): normal group, NOA group, and three Shengjing capsule treatment groups (low-dose, medium-dose, and high-dose groups, respectively). HE staining and semen smear were performed to assess sperm quality. The expression levels of PI3K/AKT and integrin α6/β1 were measured by qRT-PCR and western blot analyses. Results. In the NOA group, almost all of the seminiferous tubules were vacuolated with a thin layer of basal compartment containing some spermatogonial stem cells. The counts of sperms in the NOA group were strongly lower than those of the normal group (P=0.0001). The expression of PI3K/AKT and integrin α6/β1 was scarcely expressed in the NOA group. All indexes mentioned above were significantly different from those of the medium- and high-dose groups (P=0.001, all). The sperm count of rats treated with Shengjing capsules was significantly higher than that of the NOA group (P=0.0001). The rats of Shengjing capsule groups had more layers of spermatogonial stem cells and spermatocytes, and some had intracavitary sperms. Conclusions. Shengjing capsules may be a promising therapeutic medicine for NOA. The underlying mechanisms might involve activating SSCs by upregulating the integrin α6/β1 expression via the PI3K/AKT pathway.

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