Functional Protein-Based Bioinspired Nanomaterials: From Coupled Proteins, Synthetic Approaches, Nanostructures to Applications

Protein-based bioinspired nanomaterials (PBNs) combines the advantage of the size, shape, and surface chemistry of nanomaterials, the morphology and functions of natural materials, and the physical and chemical properties of various proteins. Recently, there are many exciting developments on biomimetic nanomaterials using proteins for different applications including, tissue engineering, drug delivery, diagnosis and therapy, smart materials and structures, and water collection and separation. Protein-based biomaterials with high biocompatibility and biodegradability could be modified to obtain the healing effects of natural organisms after injury by mimicking the extracellular matrix. For cancer and other diseases that are difficult to cure now, new therapeutic methods involving different kinds of biomaterials are studied. The nanomaterials with surface modification, which can achieve high drug loading, can be used as drug carriers to enhance target and trigger deliveries. For environment protection and the sustainability of the world, protein-based nanomaterials are also applied for water treatment. A wide range of contaminants from natural water source, such as organic dyes, oil substances, and multiple heavy ions, could be absorbed by protein-based nanomaterials. This review summarizes the formation and application of functional PBNs, and the details of their nanostructures, the proteins involved, and the synthetic approaches are addressed.

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Development of liposomal drug formulations: quality attributes and methods for quality control

Bulletin of Russian State Medical University ◽

10.24075/brsmu.2018.092 ◽

2018 ◽

pp. 33-39

Author(s):

E.V. Melnikova ◽

D.V. Goryachev ◽

A.A. Chaplenko ◽

M.A. Vodyakova ◽

A.R. Sayfutdinova ◽

...

Keyword(s):

Quality Control ◽

Gel Filtration ◽

Chemical Properties ◽

Drug Carriers ◽

Quality Attributes ◽

Scanning Calorimetry ◽

Liposomal Drug ◽

Wide Range ◽

Drug Manufacturing ◽

Major Trend

The use of nanostructured components in drug manufacturing and, more specifically, targeted drug delivery has recently become a major trend in the pharmaceutical industry. Nanodrugs encompass a wide range of pharmaceutical agents containing dendrimers, nanocrystals, micelles, liposomes, and polymer nanoparticles. Liposomes are the most well-studied nanoparticles and effective drug carriers. However, the more complex their structure is, the more process controls are needed and the more quality attributes have to be monitored, including the chemical properties of the liposomal fraction such as the shape, size and charge of the nanoparticle, conjugation efficacy, and distribution of the active ingredient. We believe that quality control of key liposome characteristics should rely on dynamic and laser light scattering coupled with electrophoresis, differential scanning calorimetry, cryo-electron microscopy, nuclear magnetic resonance, laser diffraction analysis, and gel filtration chromatography.

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The state of cognitive functions after angioreconstructive operations on the carotid arteries

Bulletin of Russian State Medical University ◽

10.24075/brsmu.2019.059 ◽

2019 ◽

pp. 65-71

Author(s):

M.M. Tanashyan ◽

R.B. Medvedev ◽

O.V. Lagoda ◽

E.S. Berdnikovich ◽

S.I. Skrylev ◽

...

Keyword(s):

Gel Filtration ◽

Chemical Properties ◽

Drug Carriers ◽

Scanning Calorimetry ◽

Wide Range ◽

Drug Manufacturing ◽

Major Trend ◽

Pharmaceutical Agents ◽

Process Controls ◽

Resonance Laser

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Brief history and ways of further development of the department of hydraulic engineering and hydraulics FSBI «VNIIGiM named after A.N. Kostyakov»

Melioration and Water Management ◽

10.32962/0235-2524-2019-5-18-23 ◽

2020 ◽

pp. 18-23

Author(s):

Alexey Shcherbakov ◽

Valentin Zhezmer

Keyword(s):

Land Reclamation ◽

Water Source ◽

Water Sources ◽

Hydraulic Engineering ◽

Negative Effects ◽

Extensive Experience ◽

Safety Level ◽

Theoretical Substantiation ◽

Wide Range ◽

Construction Operation

Department of hydraulic engineering and hydraulics FGBNU «VNIIGiM them. A.N. Kostyakova «has a long history. For many years, the department’s staff has been such scientists and water engineers with extensive experience as M.A. Volynov, V.S. Verbitsky, S.S. Medvedev, N.V. Lebedev, B.C. Panfilov, T.G. Voynich-Syanozhentsky, V.A. Golubkova, G.V. Lyapin and others. The department solved a wide range of tasks, the main areas of research were the following: – theoretical and applied hydrodynamics and hydraulics, with reference to the open channel flows that affect the state and level of safety of the hydraulic structures; – integrated use and protection of water bodies – water sources and water sources of water resources used in land reclamation; – development of measures and technical solutions for the protection of objects from the negative effects of water; – theoretical substantiation of works to improve the safety level of the GTS (declaration); – development and implementation of digitalization methods for solving design, construction, operation and control of landreclamation facilities. Currently, promising areas of research is the development of a decision-making algorithm in the designation of measures to rationalize the provision of resources to water amelioration. The algorithm is developed on the basis of a detailed study, systematization and processing of data both on safety and on the efficiency of systems and structures, ensuring the delivery of irrigation water of the required quality and in sufficient quantity from a water source to the field.

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Advances in the use of MOFs for Cancer Diagnosis and Treatment: An Overview

Current Pharmaceutical Design ◽

10.2174/1381612826666200406153949 ◽

2020 ◽

Vol 26 (33) ◽

pp. 4174-4184

Author(s):

Marina P. Abuçafy ◽

Bruna L. da Silva ◽

João A. Oshiro-Junior ◽

Eloisa B. Manaia ◽

Bruna G. Chiari-Andréo ◽

...

Keyword(s):

Drug Delivery ◽

Drug Delivery Systems ◽

Rational Design ◽

Gas Adsorption ◽

Drug Loading ◽

Drug Carriers ◽

Delivery Systems ◽

Academic Community ◽

Anticancer Drug Delivery ◽

Diagnostic Agents

Nanoparticles as drug delivery systems and diagnostic agents have gained much attention in recent years, especially for cancer treatment. Nanocarriers improve the therapeutic efficiency and bioavailability of antitumor drugs, besides providing preferential accumulation at the target site. Among different types of nanocarriers for drug delivery assays, metal-organic frameworks (MOFs) have attracted increasing interest in the academic community. MOFs are an emerging class of coordination polymers constructed of metal nodes or clusters and organic linkers that show the capacity to combine a porous structure with high drug loading through distinct kinds of interactions, overcoming the limitations of traditional drug carriers explored up to date. Despite the rational design and synthesis of MOFs, structural aspects and some applications of these materials like gas adsorption have already been comprehensively described in recent years; it is time to demonstrate their potential applications in biomedicine. In this context, MOFs can be used as drug delivery systems and theranostic platforms due to their ability to release drugs and accommodate imaging agents. This review describes the intrinsic characteristics of nanocarriers used in cancer therapy and highlights the latest advances in MOFs as anticancer drug delivery systems and diagnostic agents.

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The Potential of Milk-derived Exosomes for Drug Delivery

Current Drug Delivery ◽

10.2174/1567201817666200817112503 ◽

2020 ◽

Vol 17 ◽

Author(s):

Shuyuan Li ◽

Yue Tang ◽

Yushun Dou

Keyword(s):

Drug Delivery ◽

Oral Delivery ◽

Therapeutic Potential ◽

Current Knowledge ◽

Biological Fluids ◽

Drug Loading ◽

Drug Carriers ◽

Current Application ◽

Therapeutic Benefits ◽

Loading Methods

Background: Exosomes, one of the extracellular vesicles, are widely present in all biological fluids and play an important role in intercellular communication. Because of its hydrophobic lipid bilayer and aqueous hydrophilic core structure, it is considered a possible alternative to liposome drug delivery systems. Not only do they protect the cargo like liposomes during delivery, they are less toxic and better tolerated. However, due to the lack of sources and methods for obtaining enough exosomes, the therapeutic application of exosomes as drug carriers is limited. Methods: A literature search was performed using the ScienceDirect and PubMed electronic databases to obtain information from published literature on milk exosomes related to drug delivery. Results: Here, we briefly reviewed the current knowledge of exosomes, expounded the advantages of milk-derived exosomes over other delivery vectors, including a higher yield, the oral delivery characteristic and additional therapeutic benefits. The purification and drug loading methods of milk exosomes, and the current application of milk exosomes were also introduced. Conclusion: The emergence of milk-derived exosomes is expected to break through the limitations of exosomes as therapeutic carriers of drugs. We hope to raise awareness of the therapeutic potential of milk-derived exosomes as a new drug delivery system.

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Hyaluronan based Multifunctional Nano-carriers for Combination Cancer Therapy

Current Topics in Medicinal Chemistry ◽

10.2174/1568026620666200922113846 ◽

2020 ◽

Vol 20 ◽

Author(s):

Menghan Gao ◽

Hong Deng ◽

Weiqi Zhang

Keyword(s):

Cancer Therapy ◽

Combination Therapy ◽

Cancer Cells ◽

Drug Loading ◽

Tumor Therapy ◽

Drug Carriers ◽

Functional Sites ◽

Therapeutic Modalities ◽

Combination Cancer Therapy ◽

Targeting Strategy

: Hyaluronan (HA) is a natural linear polysaccharide that has excellent hydrophilicity, biocompatibility, biodegradability, and low immunogenicity, making it one of the most attractive biopolymers used for biomedical researches and applications. Due to the multiple functional sites on HA and its intrinsic affinity for CD44, a receptor highly expressed on various cancer cells, HA has been widely engineered to construct different drug-loading nanoparticles (NPs) for CD44- targeted anti-tumor therapy. When a cocktail of drugs is co-loaded in HA NP, a multifunctional nano-carriers could be obtained, which features as a highly effective and self-targeting strategy to combat the cancers with CD44 overexpression. The HA-based multidrug nano-carriers can be a combination of different drugs, various therapeutic modalities, or the integration of therapy and diagnostics (theranostics). Up to now, there are many types of HA-based multidrug nano-carriers constructed by different formulation strategies including drug co-conjugates, micelles, nano-gels and hybrid NP of HA and so on. This multidrug nano-carrier takes the full advantages of HA as NP matrix, drug carriers and targeting ligand, representing a simplified and biocompatible platform to realize the targeted and synergistic combination therapy against the cancers. In this review, recent progresses about HA-based multidrug nano-carriers for combination cancer therapy are summarized and its potential challenges for translational applications have been discussed.

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Stereocomplexation Assisted Assembly of Poly(γ-glutamic Acid)-graft-polylactide Nano-micelles and Their Efficacy as Anticancer Drug Carrier

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520617666170911170104 ◽

2018 ◽

Vol 18 (2) ◽

pp. 302-311

Author(s):

Shulin Dai ◽

Yucheng Feng ◽

Shuyi Li ◽

Yuxiao Chen ◽

Meiqing Liu ◽

...

Keyword(s):

Glutamic Acid ◽

Drug Carrier ◽

Drug Loading ◽

Drug Carriers ◽

Cancer Drug ◽

Drug Release Profile ◽

Sustained Drug Release ◽

Anti Cancer ◽

Physical Interactions

Background: Micelles as drug carriers are characterized by their inherent instability due to the weak physical interactions that facilitate the self-assembly of amphiphilic block copolymers. As one of the strong physical interactions, the stereocomplexation between the equal molar of enantiomeric polylactides, i.e., the poly(L-lactide) (PLLA) and poly(D-lactide) (PDLA), may be harnessed to obtain micelles with enhanced stability and drug loading capacity and consequent sustained release. </P><P> Aims/Methods: In this paper, stereocomplexed micelles gama-PGA-g-PLA micelles) were fabricated from the stereocomplexation between poly(gama-glutamic acid)-graft-PLLA gama-PGA-g-PLA) and poly(gamaglutamic acid)-graft-PDLA gama-PGA-g-PLA). These stereocomplexed micelles exhibited a lower CMC than the corresponding enantiomeric micelles. Result: Furthermore, they showed higher drug loading content and drug loading efficiency in addition to more sustained drug release profile in vitro. In vivo imaging confirmed that the DiR-encapsulated stereocomplexed gama-PGA-g-PLA micelles can deliver anti-cancer drug to tumors with enhanced tissue penetration. Overall, gama-PGA-g-PLA micelles exhibited greater anti-cancer effects as compared with the free drug and the stereocomplexation may be a promising strategy for fabrication of anti-cancer drug carriers with significantly enhanced efficacy.

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A Highly Versatile Polymer Network Based on Liquid Crystalline Dendrimers

International Journal of Molecular Sciences ◽

10.3390/ijms22115740 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5740

Author(s):

Ramón Cervera-Procas ◽

José-Luis Serrano ◽

Ana Omenat

Keyword(s):

Smart Materials ◽

Liquid Crystalline ◽

Organic Dyes ◽

Polymer Network ◽

Viscoelastic Behavior ◽

Diverse Range ◽

Polymeric Systems ◽

System A ◽

Porous Microstructure ◽

Solvent Molecules

Highly functional macromolecules with a well-defined architecture are the key to designing efficient and smart materials, and these polymeric systems can be tailored for specific applications in a diverse range of fields. Herein, the formation of a new liquid crystalline polymeric network based on the crosslinking of dendrimeric entities by the CuI-catalyzed variant of the Huisgen 1,3-dipolar cycloaddition of azides and alkynes to afford 1,2,3-triazoles is reported. The polymeric material obtained in this way is easy to process and exhibits a variety of properties, which include mesomorphism, viscoelastic behavior, and thermal contraction. The porous microstructure of the polymer network determines its capability to absorb solvent molecules and to encapsulate small molecules, like organic dyes, which can be released easily afterwards. Moreover, all these properties may be easily tuned by modifying the chemical structure of the constituent dendrimers, which makes this system a very interesting one for a number of applications.

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Palladium and Copper: Advantageous Nanocatalysts for Multi-Step Transformations

Nanomaterials ◽

10.3390/nano11081891 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1891

Author(s):

Antonio Reina ◽

Trung Dang-Bao ◽

Itzel Guerrero-Ríos ◽

Montserrat Gómez

Keyword(s):

Chemical Properties ◽

Added Value ◽

Solid Supports ◽

Nanosized Materials ◽

Nano Structures ◽

Liquid Phases ◽

Wide Range ◽

Synthetic Methodologies ◽

Physical And Chemical ◽

A Current

Metal nanoparticles have been deeply studied in the last few decades due to their attractive physical and chemical properties, finding a wide range of applications in several fields. Among them, well-defined nano-structures can combine the main advantages of heterogeneous and homogenous catalysts. Especially, catalyzed multi-step processes for the production of added-value chemicals represent straightforward synthetic methodologies, including tandem and sequential reactions that avoid the purification of intermediate compounds. In particular, palladium- and copper-based nanocatalysts are often applied, becoming a current strategy in the sustainable synthesis of fine chemicals. The rational tailoring of nanosized materials involving both those immobilized on solid supports and liquid phases and their applications in organic synthesis are herein reviewed.

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Anodic Titanium Dioxide Nanotubes for Magnetically Guided Therapeutic Delivery

Scientific Reports ◽

10.1038/s41598-019-49513-2 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 6

Author(s):

Morteza Hasanzadeh Kafshgari ◽

Delf Kah ◽

Anca Mazare ◽

Nhat Truong Nguyen ◽

Monica Distaso ◽

...

Keyword(s):

Titanium Dioxide ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Hela Cells ◽

Release Kinetics ◽

Drug Loading ◽

Oxide Nanoparticles ◽

Site Specific ◽

Therapeutic Drugs ◽

Wide Range

Abstract Hollow titanium dioxide (TiO2) nanotubes offer substantially higher drug loading capacity and slower drug release kinetics compared to solid drug nanocarriers of comparable size. In this report, we load TiO2 nanotubes with iron oxide nanoparticles to facilitate site-specific magnetic guidance and drug delivery. We generate magnetic TiO2 nanotubes (TiO2NTs) by incorporating a ferrofluid containing Ø ≈ 10 nm iron oxide nanoparticles in planar sheets of weakly connected TiO2 nanotubes. After thermal annealing, the magnetic tubular arrays are loaded with therapeutic drugs and then sonicated to separate the nanotubes. We demonstrate that magnetic TiO2NTs are non-toxic for HeLa cells at therapeutic concentrations (≤200 µg/mL). Adhesion and endocytosis of magnetic nanotubes to a layer of HeLa cells are increased in the presence of a magnetic gradient field. As a proof-of-concept, we load the nanotubes with the topoisomerase inhibitor camptothecin and achieve a 90% killing efficiency. We also load the nanotubes with oligonucleotides for cell transfection and achieve 100% cellular uptake efficiency. Our results demonstrate the potential of magnetic TiO2NTs for a wide range of biomedical applications, including site-specific delivery of therapeutic drugs.

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