scholarly journals The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains

2020 ◽  
Vol 21 (21) ◽  
pp. 8003
Author(s):  
Mateusz Mącik ◽  
Agata Gryta ◽  
Lidia Sas-Paszt ◽  
Magdalena Frąc

Regarding the unfavourable changes in agroecosystems resulting from the excessive application of mineral fertilizers, biopreparations containing live microorganisms are gaining increasing attention. We assumed that the application of phosphorus mineral fertilizer enriched with strains of beneficial microorganisms contribute to favourable changes in enzymatic activity and in the genetic and functional diversity of microbial populations inhabiting degraded soils. Therefore, in field experiments conditions, the effects of phosphorus fertilizer enriched with bacterial strains on the status of soil microbiome in two chemically degraded soil types (Brunic Arenosol—BA and Abruptic Luvisol—AL) were investigated. The field experiments included treatments with an optimal dose of phosphorus fertilizer (without microorganisms—FC), optimal dose of phosphorus fertilizer enriched with microorganisms including Paenibacillus polymyxa strain CHT114AB, Bacillus amyloliquefaciens strain AF75BB and Bacillus sp. strain CZP4/4 (FA100) and a dose of phosphorus fertilizer reduced by 40% and enriched with the above-mentioned bacteria (FA60). The analyzes performed included: the determination of the activity of the soil enzymes (protease, urease, acid phosphomonoesterase, β-glucosidase), the assessment of the functional diversity of microorganisms with the application of BIOLOGTM plates and the characterization of the genetic diversity of bacteria, archaea and fungi with multiplex terminal restriction fragment length polymorphism and next generation sequencing. The obtained results indicated that the application of phosphorus fertilizer enriched with microorganisms improved enzymatic activity, and the genetic and functional diversity of the soil microbial communities, however these effects were dependent on the soil type.

Author(s):  
L. V. Tsentylo

All agrotechnical and agrochemical measures aimed at increasing soil fertility have an effect on enzyme activity. Measures such as soil cultivation, the introduction of organic and mineral fertilizers into the soil,activate or suppress the enzymatic processes. The research relies on the intensity and direction of biochemical processes in the soil, containing a series of methods, the use of which has enabled the establishment of a favorable enzymatic active layer of arable chernozem typical. Such methods include: field, laboratory, analysis. The level of differentiation of protease, urease, phosphatase, amylase, catalase, depending on the cultivation of soil and fertilizer, was revealed in the process of comparative analysis of enzymic activity of typical black soil in sunflower agrocenosis. Field experiments on the study of fermentative activity of typical black currant were conducted during 2012-2016. The object of research was soil cover. It was established that the activation of biochemical processes in the soil is ensured by the organo-mineral fertilizer system - compost 4.5 t + N40P48K54 + 3.5 t by-products and seed weight per hectare of crop rotational area. Application of the mineral fertilizer system reduces the enzymatic activity of typical black soil. The highest activity of the protease and catalase passes in the arable layer for powered-unpowered cultivation. Higher phosphatase activity was observed for differentiated soil cultivation. Research on this problem should be continued in order to establish a relationship between the parameters of enzymatic activity of soil with the structure of microbial cenosis of typical black soil of different systems of basic cultivation and fertilization


2019 ◽  
pp. 23-27
Author(s):  
Lyudmila Alexandrovna Razumova ◽  
Roman Aleksandrovich Kamenev ◽  
Elena Georgievna Balenko

The article presents the results of field experiments on the effect of mineral fertilizers and bacterial preparations on the yield and quality of safflower seeds. Studies were conducted in 2016-2018 in the North-Eastern zone of the Rostov region on dark chestnut soils. The object of research was the variety of safflower Zavolzhsky-1. Predecessor was winter wheat. As mineral fertilizers, ammonium nitrate and ammophos were used in various doses, according to the scheme of experience, which were introduced simultaneously with sowing or randomly for presowing cultivation. The bacterial preparations were presented to the associative nitrogen-fixing bacteria of the strains Minorin, Flavobacterium, KL-10 production research center of Pushkin. In the field experiment, the variants of the joint application of mineral fertilizers and pre-sowing treatment of seeds with bacterial preparations were also studied. The control was an option without the use of mineral fertilizers and bacterial preparations. The research methodology is standard for studying the action of fertilizers. The average seed yield of safflower on the options amounted to 1,03-1,22 t/ha, oil content of seeds of 35.9-36.3 percent, and the yield of oil yield of seeds from 1 ha – 337-398 kg. In the course of research, we have found that the optimal dose of mineral fertilizers, providing the greatest yield and yield of oil in harvest – introduction of scattering under the sowing cultivation at a dose of N48P52. The increase to the control variant was 18.4%. The yield of oil was increased by 23.7%. The bacterial preparation with associative strains of nitrogen-fixing bacteria for use on natural background fertility of the soil Flavobacterium was identified. The increase in the yield of oilseeds was 13.6%, oil collection was 18.1%.    


Author(s):  
V.V. Zinchenko ◽  
◽  
E.S Fedorenko ◽  
A.V Gorovtsov ◽  
T.M Minkina ◽  
...  

As a result of the model experiment, an increase in the enzymatic activity of meadow chernozem of the impact zone of Ataman Lake with the introduction of a strains mixture of metal-resistant microorganisms into the soil was established. The experiment has shown that the application of bacterial strains increases the dehydrogenase activity of contaminated soil by 51.8% compared to the variant without remediation


Author(s):  
M.I. Perepechai ◽  

in studies on the influence of herbicides on the weediness and yield of barley under different technologies of tillage in the Smolensk region, it was found that the Reliable barley variety formed high yields (more than 4 t / ha) during winter plowing with an optimal dose of mineral fertilizers and when using herbicides.


Author(s):  
А. М. Grebennikov ◽  
А. S. Frid ◽  
V. P. Belobrov ◽  
V. А. Isaev ◽  
V. М. Garmashоv ◽  
...  

The article assesses the relationships between the morphological properties of agrochernozems and yield of peas on the plots, experience with different methods of basic treatment (moldboard plowing at the depth of 20 - 22, 25 - 27 and 14 - 16 cm, moldboard plowing to a depth of 14 - 16 cm, combined midwater moldboard, mid-water subsurface, surface to a depth of 6 - 8 cm and zero tillage) is inherent in V.V. Dokuchaev Research Institute of Agriculture of the Central Black Earth strip, in the fall of 2014. The research was conducted in 2015 - 2016, with the application of mineral fertilizers (N60Р60К60) and unfertilized background. The highest pea yields in the fertilized as the background, and without the use of fertilizers was observed in dumping plowing and especially in the variant with deep moldboard plowing, which creates in comparison with other ways of handling the best conditions for the growth and development of peas. The lowest yield of pea was obtained with zero processing. Apparently legalistic migrational-mizelial agrochernozems the Central Chernozem zone of minimum tillage in the cultivation of peas are not effective, what is evident already in the first year after the laying of experience with different basic treatments. As shown by the results of applying multifactor analysis of variance studied the mapping properties of the soil can have the same significant impact on the yield of agricultural crops, as options for the field experiments aimed at assessing the impact of various treatments on yield.


Author(s):  
V. Dumych ◽  

The purpose of research: to improve the technology of growing flax in the Western region of Ukraine on the basis of the introduction of systems for minimizing tillage, which will increase the yield of trusts and seeds. Research methods: field, laboratory, visual and comparative calculation method. Research results: Field experiments included the study of three tillage systems (traditional, canning and mulching) and determining their impact on growth and development and yields of trusts and flax seeds. The traditional tillage system included the following operations: plowing with a reversible plow to a depth of 27 cm, cultivation with simultaneous harrowing and pre-sowing tillage. The conservation system is based on deep shelfless loosening of the soil and provided for chiseling to a depth of 40 cm, disking to a depth of 15 cm, cultivation with simultaneous harrowing, pre-sowing tillage. During the implementation of the mulching system, disking to a depth of 15 cm, cultivation with simultaneous harrowing and pre-sowing tillage with a combined unit was carried out. Tillage implements and machines were used to perform tillage operations: disc harrow BDVP-3,6, reversible plow PON-5/4, chisel PCh-3, cultivator KPSP-4, pre-sowing tillage unit LK-4. The SZ-3,6 ASTPA grain seeder was used for sowing long flax of the Kamenyar variety. Simultaneously with the sowing of flax seeds, local application of mineral fertilizers (nitroammophoska 2 c/ha) was carried out. The application of conservation tillage allows to obtain the yield of flax trust at the level of 3,5 t/ha, which is 0,4 t/ha (12.9 %) more than from the area of traditional tillage and 0,7 t/ha (25 %) in comparison with mulching. In the area with canning treatment, the seed yield was the highest and amounted to 0,64 t/ha. The difference between this option and traditional and mulching tillage reaches 0,06 t/ha (10,3 %) and 0.10 t/ha (18.5 %), respectively. Conclusions. Preservation tillage, which is based on shelf-free tillage to a depth of 40 cm and disking to a depth of 15 cm has a positive effect on plant growth and development, yield and quality of flax.


Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 695
Author(s):  
Oleg Bazaluk ◽  
Valerii Havrysh ◽  
Mykhailo Fedorchuk ◽  
Vitalii Nitsenko

Global climate changes and fossil fuel reserve depletion are drivers for the search for environmentally friendly renewable energy sources. In Europe, biomass represents the main alternative to fossil fuels. Among energy crops, sorghum is a promising crop for arid regions. The biomass yield and energy efficiency of sorghum (both silage and grain) were studied based on field experiments conducted in Southern Ukraine. The following climate changes were identified: an increase in temperature and a decrease in precipitation. The total energy inputs for sweet sorghum were estimated at 11.256 GJ/ha. The main contributors to the energy inputs are mineral fertilizers (56.99%). The experiment showed that a yield of 40.6 t/ha could be achieved with annual precipitation of 350 mm. The energy efficiency ratio was determined to be 11.18. The total energy inputs for grain sorghum was 16.081 GJ/ha. Its yield (grain) varied from 1.92 to 7.05 t/ha. The energy efficiency ratio of grain sorghum ranged from 2.8 to 16.7.


2021 ◽  
Vol 9 (7) ◽  
pp. 1400
Author(s):  
Marta Bertola ◽  
Andrea Ferrarini ◽  
Giovanna Visioli

Soil is one of the key elements for supporting life on Earth. It delivers multiple ecosystem services, which are provided by soil processes and functions performed by soil biodiversity. In particular, soil microbiome is one of the fundamental components in the sustainment of plant biomass production and plant health. Both targeted and untargeted management of soil microbial communities appear to be promising in the sustainable improvement of food crop yield, its nutritional quality and safety. –Omics approaches, which allow the assessment of microbial phylogenetic diversity and functional information, have increasingly been used in recent years to study changes in soil microbial diversity caused by agronomic practices and environmental factors. The application of these high-throughput technologies to the study of soil microbial diversity, plant health and the quality of derived raw materials will help strengthen the link between soil well-being, food quality, food safety and human health.


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