scholarly journals Electrodialysis Extraction of Pufferfish Skin (Takifugu flavidus): A Promising Source of Collagen

Marine Drugs ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 25 ◽  
Author(s):  
Junde Chen ◽  
Min Li ◽  
Ruizao Yi ◽  
Kaikai Bai ◽  
Guangyu Wang ◽  
...  
Keyword(s):  
Dry Weight ◽  
Extraction Process ◽  
Extraction Yield ◽  
Composition Analysis ◽  
Fishing Industry ◽  
Alternative Source ◽  
Skin Collagen ◽  
Low Efficiency ◽  
Commercial Applications ◽  
Promising Source

Collagen is widely used in drugs, biomaterials, foods, and cosmetics. By-products of the fishing industry are rich sources of collagen, which can be used as an alternative to collagen traditionally harvested from land mammals. However, commercial applications of fish-based collagen are limited by the low efficiency, low productivity, and low sustainability of the extraction process. This study applied a new technique (electrodialysis) for the extraction of Takifugu flavidus skin collagen. We found electrodialysis to have better economic and environmental outcomes than traditional dialysis as it significantly reduced the purification time and wastewater (~95%) while maintaining high extraction yield (67.3 ± 1.3 g/100 g dry weight, p < 0.05). SDS-PAGE, amino acid composition analysis, and spectrophotometric characterization indicated that electrodialysis treatment retained the physicochemical properties of T. flavidus collagen. Heavy metals and tetrodotoxin analyses indicated the safety of T. flavidus collagen. Notably, the collagen had similar thermal stability to calf skin collagen, with the maximum transition temperature and denaturation temperature of 41.8 ± 0.35 and 28.4 ± 2.5 °C, respectively. All evidence suggests that electrodialysis is a promising technique for extracting collagen in the fishing industry and that T. flavidus skin collagen could serve as an alternative source of collagen to meet the increasing demand from consumers.

Collagen from Diamondback Squid (Thysanoteuthis rhombus) Outer Skin

2004 ◽  
Vol 59 (3-4) ◽  
pp. 271-275 ◽  
Author(s):  
Takeshi Nagai
Keyword(s):  
Dry Weight ◽  
Development Stage ◽  
Sea Bream ◽  
Peptide Fragments ◽  
Alternative Source ◽  
Outer Skin ◽  
Collagen Denaturation ◽  
Skin Collagen ◽  
A Chain ◽  
Sea Fish

Collagens (acid-solubilized and pepsin-solubilized collagens) were prepared from diamondback squid outer skin and partially characterized. The yields of acid-solubilized and pepsin-solubilized collagens were about 1.3 and 35.6%, respectively, on a dry weight basis. Pepsin-solubilized collagen was heterotrimer with a chain composition of α1α2α3. The patterns of peptide fragments were different from that of porcine skin collagen. Denaturation temperature was 27.5 °C, about 10 °C lower than that of porcine collagen. The amino acid composition of pepsin-solubilized collagen from diamondback squid outer skin was similar to that from cuttlefish outer skin. This squid is big among squid species, and its skin is thick. It is clear that diamondback squid outer skin has a potential as an alternative source of collagen to bovine skin and bone. At present, collagen using aquatic materials such as skin (cod and a deep-sea fish) and scale (sea bream and anchovy) is the development stage in the related industries. Unless the problem of BSE infection in land animals is resolved aquatic materials as an alternative source of collagen will attract much attention in the cosmetic and medical fields.

scholarly journals Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB)

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Beatrice Mongili ◽  
Annalisa Abdel Azim ◽  
Silvia Fraterrigo Garofalo ◽  
Esperanza Batuecas ◽  
Angela Re ◽  
...  
Keyword(s):  
Dimethyl Carbonate ◽  
Mixing Time ◽  
Research Question ◽  
Health Impact ◽  
Extraction Process ◽  
Extraction Yield ◽  
Experimental Conditions ◽  
Alternative Source ◽  
A Minor ◽  
The Impact

Abstract Background Plastic plays a crucial role in everyday life of human living, nevertheless it represents an undeniable source of land and water pollution. Polyhydroxybutyrate (PHB) is a bio-based and biodegradable polyester, which can be naturally produced by microorganisms capable of converting and accumulating carbon as intracellular granules. Hence, PHB-producing strains stand out as an alternative source to fossil-derived counterparts. However, the extraction strategy affects the recovery efficiency and the quality of PHB. In this study, PHB was produced by a genetically modified Escherichia coli strain and successively extracted using dimethyl carbonate (DMC) and ethanol as alternative solvent and polishing agent to chloroform and hexane. Eventually, a Life Cycle Assessment (LCA) study was performed for evaluating the environmental and health impact of using DMC. Results Extraction yield and purity of PHB obtained via DMC, were quantified, and compared with those obtained via chloroform-based extraction. PHB yield values from DMC-based extraction were similar to or higher than those achieved by using chloroform (≥ 67%). To optimize the performance of extraction via DMC, different experimental conditions were tested, varying the biomass state (dry or wet) and the mixing time, in presence or in absence of a paper filter. Among 60, 90, 120 min, the mid-value allowed to achieve high extraction yield, both for dry and wet biomass. Physical and molecular dependence on the biomass state and solvent/antisolvent choice was established. The comparative LCA analysis promoted the application of DMC/ethanol rather than chloroform/hexane, as the best choice in terms of health prevention. However, an elevated impact score was achieved by DMC in the environmental-like categories in contrast with a minor contribution by its counterpart. Conclusion The multifaceted exploration of DMC-based PHB extraction herein reported extends the knowledge of the variables affecting PHB purification process. This work offers novel and valuable insights into PHB extraction process, including environmental aspects not discussed so far. The findings of our research question the DMC as a green solvent, though also the choice of the antisolvent can influence the impact on the examined categories.

scholarly journals Fucoxanthin and Phenolic Contents of Six Dictyotales From the Tunisian Coasts With an Emphasis for a Green Extraction Using a Supercritical CO2 Method

2021 ◽  
Vol 8 ◽  
Author(s):  
Leila Ktari ◽  
Cheima Mdallel ◽  
Brahim Aoun ◽  
Leila Chebil Ajjabi ◽  
Saloua Sadok
Keyword(s):  
High Performance ◽  
Dry Weight ◽  
Abundant Species ◽  
Antioxidant Property ◽  
Extraction Yield ◽  
Added Value ◽  
Bioactive Metabolites ◽  
Total Phenol Content ◽  
Dictyota Dichotoma ◽  
Promising Source

Dictyotales, a common taxonomic group of brown seaweeds found in warm and temperate waters, are known for their richness in bioactive metabolites. In this study, six species of Dictyotales (Dictyopteris polypodioides, Dictyota dichotoma, Dictyota fasciola, Dictyota spiralis, Padina pavonica, and Taonia atomaria) collected from the Tunisian coasts were investigated for their antioxidant potentials, based on their contents of high added-value bioactive metabolites such as fucoxanthin and polyphenols. Fucoxanthin and polyphenols were analyzed quantitatively by high-performance liquid chromatography (HPLC) and UV spectrophotometer, respectively. The antioxidant property of extracts was also determined based on their ability to scavenge 2,2-Diphenyl-1-picrylhydrazyl (DPPH’s) free radical. Thus, the highest concentrations of fucoxanthin were obtained from T. atomaria and D. polypodioides (5.53 ± 1.2 and 3.43 ± 1.3 mg⋅g–1 dry weight, respectively), while the lowest amount was from D. spiralis (0.23 ± 0.1 m mg⋅g–1 dry weight). Dictyota dichotoma and T. atomaria gave the highest total phenol content (19.3 ± 0.4 and 15.2 ± 1.1 mg GAE⋅g–1 dry weight, respectively). In the second step, supercritical carbon dioxide (ScCO2) was used as a green and environmentally safe technique for the extraction of fucoxanthin from the most abundant species D. polypodioides. The extraction of fucoxanthin by ScCO2 allowed an extraction yield ranging from 0.50 ± 0.04% to 1.32 ± 0.02%, with 60°C temperature and 50-MPa pressure as the best extraction conditions. The maximum fucoxanthin and polyphenol recovery in the extract attained 15 and 64%, respectively. The results strengthen the possible use of Dictyotales from the Mediterranean Sea as a promising source of natural ingredients of health and economic interests contributing to Blue Growth in the region.

scholarly journals Longevity of Raw and Lyophilized Crude Urease Extracts

2021 ◽  
Vol 2 (2) ◽  
pp. 325-334
Author(s):  
Neda Javadi ◽  
Hamed Khodadadi Tirkolaei ◽  
Nasser Hamdan ◽  
Edward Kavazanjian
Keyword(s):  
Crude Extract ◽  
Room Temperature ◽  
Low Cost ◽  
Extraction Process ◽  
Crude Extracts ◽  
Simple Extraction ◽  
Commercial Applications ◽  
Stable Source ◽  
The Stability ◽  
The Cost

The stability (longevity of activity) of three crude urease extracts was evaluated in a laboratory study as part of an effort to reduce the cost of urease for applications that do not require high purity enzyme. A low-cost, stable source of urease will greatly facilitate engineering applications of urease such as biocementation of soil. Inexpensive crude extracts of urease have been shown to be effective at hydrolyzing urea for carbonate precipitation. However, some studies have suggested that the activity of a crude extract may decrease with time, limiting the potential for its mass production for commercial applications. The stability of crude urease extracts shown to be effective for biocementation was studied. The crude extracts were obtained from jack beans via a simple extraction process, stored at room temperature and at 4 ℃, and periodically tested to evaluate their stability. To facilitate storage and transportation of the extracted enzyme, the longevity of the enzyme following freeze drying (lyophilization) to reduce the crude extract to a powder and subsequent re-hydration into an aqueous solution was evaluated. In an attempt to improve the shelf life of the lyophilized extract, dextran and sucrose were added during lyophilization. The stability of purified commercial urease following rehydration was also investigated. Results of the laboratory tests showed that the lyophilized crude extract maintained its activity during storage more effectively than either the crude extract solution or the rehydrated commercial urease. While incorporating 2% dextran (w/v) prior to lyophilization of the crude extract increased the overall enzymatic activity, it did not enhance the stability of the urease during storage.

Preliminary Study of Alginates Extracted from Brown Algae (Sargassum sp.) Available in Madura Island as Composite Based Hydrogel Materials

2019 ◽  
Vol 964 ◽  
pp. 240-245 ◽  
Author(s):  
Amaliya Rasyida ◽  
Thalyta Rizkha Pradipta ◽  
Sigit Tri Wicaksono ◽  
Vania Mitha Pratiwi ◽  
Yeny Widya Rakhmawati
Keyword(s):  
Sodium Alginate ◽  
Brown Algae ◽  
Extraction Process ◽  
Composition Analysis ◽  
3D Printer ◽  
X Ray Diffraction ◽  
X Ray ◽  
3D Printed ◽  
Preliminary Study

Utilization of brown algae especially in Madura, where it’s close to Surabaya, only limited for food. This become a reference for developing and increasing the potential of this algae by extracting one of the ingredients, namely alginate. This paper deals with the characterization of sodium alginate extracted from sargassum sp. using modified-purified calcium routes. The extracted sodium alginate will be further used as composite hydrogel materials and compared with commercial sodium alginate. Hereafter, the synthesized composite is expected to be bio-ink for 3d printer. Chemical composition analysis were analyzed using X-Ray Fluorosense (XRF) followed by Fourier-transform infrared spectroscopy (FTIR) analysis to identify the functional group of composite and X-Ray Diffraction (XRD). Furthermore, viscosity bath is performed to compare the viscosity of extracted and commercial one. The result shows that modified-purified calcium routes in the extraction process of sodium alginate is desirable for improving their properties. Interestingly enough, with the goal of using it as bio-ink in 3d printed fabrication, the synthesized composite shows viscosity, 300 cSt, which meets the criteria for bio-ink in 3d printer.

Extraction Process of Amaranthine Pigment Keeping the Water Wax Fuit Biological Active

2013 ◽  
Vol 295-298 ◽  
pp. 150-154
Author(s):  
Feng Jie Wang ◽  
Xin Qiao Dong ◽  
Xiao Liu
Keyword(s):  
Citric Acid ◽  
Extraction Method ◽  
Raw Materials ◽  
Extraction Process ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Natural Pigment ◽  
Pigment Extraction ◽  
Acid Aqueous Solution ◽  
The Stability

Development natural pigment retained a variety of nutrition composition is extremely research value.Water Wax Trees Fruit was as raw materials,citric acid aqueous solution was as extracting agent.Amaranthine pigment extraction method were studied on fully grasping the stability of the natural amaranthine pigment and no damage to the water wax fruit biological activity.The effect of extraction yield were studied on Water Wax Trees Fruit natural amaranthine pigment.The results show that the better extraction conditions are extraction temperature at 70 °C, extraction time for 3h,citric acid concentration1.0 % , the material/liquid ration(g/ml) 1:5.The extraction yield is 2.95%.Ultraviolet absorption maximum wavelength is 280 nm.

Separation of Carotenoids from Physalis Alkekengi var. Francheti through Supercritical CO2

2011 ◽  
Vol 236-238 ◽  
pp. 2414-2417 ◽  
Author(s):  
Zhen Huang ◽  
Mei Jing Yang ◽  
Qing Ma ◽  
Shao Fang Liu
Keyword(s):  
Particle Size ◽  
Diffusion Layer ◽  
Processing Time ◽  
Extraction Process ◽  
Theory Model ◽  
Extraction Yield ◽  
Total Carotenoid ◽  
Relative Deviation ◽  
Time Profiles

Separation of carotenoids has been carried out on the calyx of Physalis alkekengi var. francheti by means of supercritical CO2. The influences of various parameters such as extraction pressure, temperature, processing time and particle size on the total carotenoid yield extracted are investigated in detail. A diffusion layer theory model is used to correlate the extraction yield vs. time profiles and it performs rather satisfactorily to describe the extraction process with an average absolute relative deviation varying from 3.2 to 11.0%.

scholarly journals DETERMINATION OF PROPERTIES AND ELEMENTAL COMPOSITION OF MUNICIPAL SOLID WASTE IN ORDER TO PROPOSAL OF WASTE-TO-ENERGY PROJECT IN BINH DUONG PROVINCE

2018 ◽  
Vol 54 (2A) ◽  
pp. 56
Author(s):  
Phung Chi Vy
Keyword(s):  
Solid Waste ◽  
Elemental Composition ◽  
Calorific Value ◽  
Dry Weight ◽  
Solid Wastes ◽  
Waste To Energy ◽  
Composition Analysis ◽  
Energy Plant ◽  
Inorganic Components

Domestic solid wastes are classified into 10 samples of 04 groups with different sizes: 2 samples with sizes under and over 120 mm (M1-1, M1-2); 2 samples with sizes under and over 80 mm (M2-1, M2-2); 2 samples with sizes under and over 40 mm (M3-1, M3-2); 4 samples with sizes under 40 mm, 40 to 80 mm, 80 to 120 mm and over 120 mm (M4-1, M4-2, M4-3, M4-4). Results of sorting 10 solid waste samples into food, cloth, wood, plastic, paper, rubber/leather, metal, glass, other organic and inorganic components shown that recycled combustible, non-recycled combustible portions are ranged from 15,46 to 93,90 %, from 5,34 to 80,17 %, respectively. The density of 10 compressed garbage samples is ranged from 525,9 to 2016,7 kg/m3; moisture contents are ranged from 18.03 to 20.92 %. Ash content is ranged from 1.12 to 9.49 % dry weight; Calorific value is ranged from 3164,9 to 5757,0 kcal/kg of garbage. The volume of leached water from 10 kg wet garbage pressed by 250 kg load in 2 days is 300 ml (equivalent to 327,1 g). Results of elemental composition analysis shown that the contents of C, H, N, Cl, S are ranged from 35,00 to 51,96, from 6,01 to 6,23, from 0,41 to 0,88, from 0,44 to 0,56, from 0,14 to 0,84 %, respectively. On this basis, the author have proposed a waste-to-energy plant with capacity of 250 tons of waste/day to generate the electricity with capacity of 17,0 MW/day.

scholarly journals Physiological quality of pink pepper seeds (Schinus terebinthifolia Raddi.) as a function of storage

2021 ◽  
Vol 38 (1) ◽  
pp. 144-157
Author(s):  
Thaísa De Oliveira Silveira ◽  
Madelon Rodrigues Braz ◽  
Gilmara Pires de Moura Palermo ◽  
Tiago Böer Breier
Keyword(s):  
Moisture Content ◽  
Storage Time ◽  
Storage Period ◽  
Seed Storage ◽  
Dry Weight ◽  
Native Forest ◽  
Alternative Source ◽  
Physiological Quality ◽  
And Storage

Seeds of native forest species for food and seedling production have a growing demand that impacts the need for studies involving seed storage in order to maintain good levels of germination quality. Thus, scientific analyzes that explore the physiological potential of the seeds of these species are necessary, especially for the pink pepper (Schinus terebinthifolia Raddi.), a species used as an alternative source of income for traditional communities and which has been arousing interest due to the consumption of its fruits as a condiment in the national and international market. This work aimed to assess the physiological quality of pink pepper seeds according to the storage period and seed size. The seeds came from a rural settlement, a pioneer in the extraction of pink pepper, in the state of Rio de Janeiro, Brazil. The seeds were evaluated for moisture content, germination, first count, length and dry weight of the germinated seedlings, during the period of time that they remained stored and the size of the seeds. The results allowed to conclude that: i) the germinative percentage of the stored pink pepper seeds decreased over the months; ii) the moisture content of the seeds decreased over the months evaluated iii) the different sizes of seeds did not show differences in germination and storage time and iv) values of length and dry weight of the seedlings did not differ according to the period of storage.

scholarly journals Lipid Extraction fromSpirulinasp. andSchizochytriumsp. Using Supercritical CO2with Methanol

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Shihong Liu ◽  
Husam A. Abu Hajar ◽  
Guy Riefler ◽  
Ben J. Stuart
Keyword(s):  
Organic Solvents ◽  
Methylene Chloride ◽  
Lipid Extraction ◽  
Volume Ratio ◽  
Soxhlet Extraction ◽  
Extraction Process ◽  
Extraction Yield ◽  
Biodiesel Production ◽  
Operating Pressure ◽  
High Lipid

Microalgae are one of the most promising feedstocks for biodiesel production due to their high lipid content and easy farming. However, the extraction of lipids from microalgae is energy intensive and costly and involves the use of toxic organic solvents. Compared with organic solvent extraction, supercritical CO2(SCCO2) has demonstrated advantages through lower toxicity and no solvent-liquid separation. Due to the nonpolar nature of SCCO2, polar organic solvents such as methanol may need to be added as a modifier in order to increase the extraction ability of SCCO2. In this paper, pilot scale lipid extraction using SCCO2was studied on two microalgae species:Spirulinasp. andSchizochytriumsp. For each species, SCCO2extraction was conducted on 200 g of biomass for 6 h. Methanol was added as a cosolvent in the extraction process based on a volume ratio of 4%. The results showed that adding methanol in SCCO2increased the lipid extraction yield significantly for both species. Under an operating pressure of 4000 psi, the lipid extraction yields forSpirulinasp. andSchizochytriumsp. were increased by 80% and 72%, respectively. It was also found that a stepwise addition of methanol was more effective than a one-time addition. In comparison with Soxhlet extraction using methylene chloride/methanol (2:1, v/v), the methanol-SCCO2extraction demonstrated its high effectiveness for lipid extraction. In addition, the methanol-SCCO2system showed a high lipid extraction yield after increasing biomass loading fivefold, indicating good potential for scaling up this method. Finally, a kinetic study of the SCCO2extraction process was conducted, and the results showed that methanol concentration in SCCO2has the strongest influence on the lipid extraction yield.

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