scholarly journals Plasma Metabolites, Productive Performance and Rumen Volatile Fatty Acid Profiles of Northern Australian Bos indicus Steers Supplemented with Desmanthus and Lucerne

Metabolites ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 356
Author(s):  
Bénédicte Suybeng ◽  
Edward Charmley ◽  
Christopher P. Gardiner ◽  
Bunmi S. Malau-Aduli ◽  
Aduli E.O. Malau-Aduli
Keyword(s):  
Growth Performance ◽  
Dry Matter ◽  
Bos Indicus ◽  
Productive Performance ◽  
Animal Performance ◽  
Rhodes Grass ◽  
N Utilization ◽  
Metabolite Profiles ◽  
Plasma Metabolite ◽  
The Impact

The hypothesis tested was that tropical steers supplemented with the Desmanthus legume and lucerne, a widely characterized temperate legume of high nutritive value, would elicit similar responses in plasma metabolite profiles, productive performance, nitrogen retention, and volatile fatty acids (VFA). The tannin-binding compound, polyethylene glycol-4000 (PEG), was added to the diets (160 g/kg Desmanthus dry matter) with the objective of further exploring nitrogen (N) utilization in the animals supplemented with Desmanthus relative to lucerne. From February to June 2020, sixteen yearling Brangus steers (average liveweight of 232 ± 6 kg) were fed a background diet of Rhodes grass (Chloris gayana) hay for 28 days, before introducing three Desmanthus cultivars (Desmanthus virgatus cv. JCU2, D. bicornutus cv. JCU4, D. leptophyllus cv. JCU7) and lucerne (Medicago sativa) at 30% dry matter intake (DMI). Relative to the backgrounding period, all supplemented steers exhibited similar growth performance. Steers supplemented with Desmanthus recorded a lower DMI and animal growth performance, but higher fecal N concentration than animals supplemented with lucerne. Among the three Desmanthus cultivars, there were no significant differences in N concentrations, VFA, and plasma metabolite profiles. The addition of PEG induced higher rumen iso-acid concentrations and fecal N excretion. However, feeding Desmanthus spp. to tropical Bos indicus steers could be a valuable means of increasing N utilization, which is attributable to the presence of tannins, and, consequently, improve animal productive performance. Since supplementation with lucerne resulted in higher liveweight, daily liveweight gains, and overall animal performance than supplementing with Desmanthus, the tested hypothesis that both supplements will elicit similar animal performance does not hold and must be rejected. Further in vivo investigation is needed to better understand the impact of tannins in Desmanthus on N utilization.

scholarly journals Impact of feeding cover crop forage containing brassicas to steers during backgrounding on live animal performance, carcass characteristics, and meat color

2021 ◽  
Author(s):  
C E Bakker ◽  
L M Hite ◽  
C L Wright ◽  
D W Brake ◽  
A J Smart ◽  
...  
Keyword(s):  
Cover Crops ◽  
Cover Crop ◽  
Dry Matter ◽  
Carcass Characteristics ◽  
Ground Beef ◽  
Dry Matter Intake ◽  
Feed Ratio ◽  
Animal Performance ◽  
Live Animal ◽  
The Impact

Abstract Brassica cover crops are an option for producers to incorporate into their cropping system to improve soil health and also provide a feed resource for cattle. While brassica cover crops have been widely used for grazing cows, their use as a backgrounding feedstuff is relatively unknown. The objective of this study was to determine the impact of feeding a brassica cover crop mixture during backgrounding on live animal performance and carcass characteristics. Thirty Angus-based steers were assigned to one of two dietary treatments during backgrounding 1) ad libitum access to a diet containing freshly cut brassica cover crop forage (CC) containing radish, turnip, rapeseed, rye grass and a liquid supplement or 2) common Midwestern dry lot growing diet containing silage, soybean meal, grass hay, and a liquid supplement (CON). Steers were assigned to electronic feed bunks (Insentec RIC, Hokofarm Group; Marknesse, Netherlands) for collection of individual feed intake. Diets were formulated to be nutritionally similar on a dry matter basis. Steers were paired by weight across treatments and pair fed. Dry matter intake was calculated daily for steers in the CC treatment and the following day CON steers were allowed access to an equal amount of dry matter using the Insentec RIC system. Steers were weighed weekly and backgrounded for 44 days before transitioning to a common finishing diet and weighed every 28 days. Steers were harvested at an estimated average backfat thickness of 1 cm. Standard carcass data were measured and strip loins and shoulder clods were collected. Instrumental and subjective color was measured on ground beef for 8 days and instrumental color was measured on strip steaks for 11 days. Treatment did not influence carcass characteristics, average daily gain, dry matter intake (P > 0.17). However, CON steers exhibited increased gain to feed ratio (P = 0.02). Additionally, a treatment by d interaction was observed for ground beef discoloration as the CC treatment displayed increased discoloration on day 4, 6, and 7 of case life (P < 0.01). These data indicate that brassicas may be utilized in a backgrounding diet without negatively impacting carcass characteristics but may decrease case life of ground beef.

Treating Bos indicus bulls with rumen transfaunation after 24 hours of transportation does not replete muscle glycogen

2016 ◽  
Vol 56 (10) ◽  
pp. 1738 ◽  
Author(s):  
C. L. O. Leo-Penu ◽  
L. A. Fitzpatrick ◽  
H. N. Zerby ◽  
A. J. Parker
Keyword(s):  
Muscle Glycogen ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Bos Indicus ◽  
Dry Matter Intake ◽  
Plasma Metabolites ◽  
Rhodes Grass ◽  
Treatment Groups ◽  
Glycogen Concentration ◽  
Ad Libitum

Twelve Bos indicus bulls were allocated to one of two treatment groups: rumen transfaunation (n = 6) given 10 kg of rumen fluid after transport or Control (n = 6), given 10 kg of deionised water after transport. Glycogen concentrations of the M. semimembranosus, M. semitendinosis and M. longisimus dorsi were measured before transport on Day –7 and after transport on Day 0, Day 1, Day 4 and Day 9. Feed intake, liveweight gain, plasma metabolites and electrolytes were recorded on the sampling days. Rumen transfaunation increased the dry matter intake of treated bulls compared with the bulls treated with deionised water. Rumen transfaunation had no effect on glycogen concentrations of the M. semimembranosus, M. semitendinosis and M. longisimus dorsi compared with the bulls treated with deionised water. The M. semimembranosus and the M. semitendinosis decreased in glycogen concentration immediately after transport on Day 0 but repleted to pre-transportation concentrations within 1 day. However, the M. longisimus dorsi demonstrated no change in glycogen concentration between the pre-transportation sample on Day –7 and Day 0 or Day 1 after transportation. The M. longisimus dorsi of the bulls increased in glycogen concentration between Day 1 and Day 4 after transportation. The bulls in this experiment demonstrated that muscle glycogen, as measured at the M. longissimus dorsi, cannot recover to sufficient concentrations (40–45 µmol of glycogen concentration per gram muscle) to ensure normal meat quality until between 1 and 4 days of rest when feeding Rhodes grass hay ad libitum.

Use of remote bunk monitoring to record effects of breed, feeding regime and weather on feeding behavior and growth performance of cattle

2003 ◽  
Vol 83 (1) ◽  
pp. 29-38 ◽  
Author(s):  
K. S. Schwartzkopf-Genswein ◽  
R. Silasi ◽  
T. A. McAllister
Keyword(s):  
Radio Frequency ◽  
Growth Performance ◽  
Radio Frequency Identification ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Dry Matter Intake ◽  
Weather Data ◽  
Feeding Regime ◽  
Time Frequency ◽  
The Impact

Thirty Charolais and 29 Holstein steers (432 ± 30 kg) blocked by weight and breed were randomly assigned to four feedlot pens equipped with radio frequency identification systems in the feed bunks. The systems monitored individual steers’ bunk attendance patterns (time, frequency, and duration of visits). Over four 21-d periods, the steers were offered (two times per day) an 80% barley grain: 20% barley silage diet for ad libitum intake (AL); restricted to 95% of their dry matter intake (DMI); during the previous 21 d; returned to an AL regime for 21 d; then restricted once again (RF). Weather data (air temperature, AT; relative humidity, RH; barometric pressure, BP; and wind speed, (WS) were expected at 1-h intervals throughout the four periods. Steer weights were recorded every 21 d; feed refusals every 7 d. Charolais steers had lower DMI (P < 0.05), higher (P < 0.005) average daily gain (ADG) and were more (P < 0.05) efficient than Holstein steers. Higher daily bunk attendance was recorded for Holstein steers during RF (P < 0.0001), and lowest for Charolais steers during RF (P < 0.0001). Dry matter intake, ADG and feed conversion were higher (v < 0.05) with AL than; with RF. Effects of weather varied with feeding regime and breed. On the AL regime, Charolais steers exhibited larger variation in daily bunk attendance than Holsteins (P < 0.0001) in relation to weather categories AT, RH and BP, but this did not compromise growth performance. Long-term data collection is required to relate the impact of weather on feeding patterns of feedlot cattle over different seasons and in different geographic locations. Key words: Feeding behaviour, feedlot, performance, thermal environment, radio frequency

The efficacy of a cultured Synergistes jonesii inoculum to control hydroxypyridone toxicity in Bos indicus steers fed leucaena/grass diets

2019 ◽  
Vol 59 (4) ◽  
pp. 696
Author(s):  
Michael J. Halliday ◽  
Hayley E. Giles ◽  
Jagadish Padmanabha ◽  
Chris S. McSweeney ◽  
Scott A. Dalzell ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Bos Indicus ◽  
Polymerase Chain Reaction Analysis ◽  
Pcr Analysis ◽  
Post Inoculation ◽  
Nucleotide Polymorphisms ◽  
Nested Polymerase Chain Reaction ◽  
Rhodes Grass ◽  
Dominant Isomer

An experiment was conducted to investigate the efficacy of a cultured Synergistes jonesii inoculum in degrading the Leucaena leucocephala (leucaena) toxins: 3-hydroxy-4(1H)-pyridone and 3-hydroxy-2(1H)-pyridone (3,4- and 2,3-DHP). Sixteen stall-housed Bos indicus steers naïve to leucaena were fed varying combinations of forage-harvested leucaena and Chloris gayana (Rhodes grass). Dietary treatments, offered at 25 g dry matter/kg LW.day, were: 25% leucaena; 50% leucaena; 100% leucaena; and 50% leucaena, switched to 50% Medicago sativa (lucerne) after 6 weeks at time of inoculation. The experiment was 10 weeks in duration, consisting of a 6-week pre-inoculation period, followed by inoculation with cultured S. jonesii, and a 4-week post-inoculation period. Mean daily dry matter intake was recorded. Twenty-four-hour urine collections and rumen fluid samples were obtained weekly for estimation of total urinary DHP, and detection of S. jonesii using nested polymerase chain reaction analysis including presence of single nucleotide polymorphisms (SNP), respectively. In the pre-inoculation period, total urinary DHP increased quickly to high levels, then gradually declined after Week 3 with 2,3-DHP the dominant isomer through to Week 6. Indigenous strains of S. jonesii were sporadically detected by PCR analysis, indicating S. jonesii was present before inoculation but at the lower limits of detection. After inoculation there was no change in the rate of total DHP degradation or the frequency of detection of S. jonesii, although there was increased rate of degradation of 2,3-DHP. SNP indicated the presence of different strains of S. jonesii in both indigenous and cultured S. jonesii. DMI was low, especially in the 100% treatment, due in part to the high stem content of the forage-harvested leucaena and probable DHP toxicosis. It was concluded that the cultured S. jonesii inoculum did not fully protect animals against leucaena toxicity.

scholarly journals Heavy-Metal Contents and the Impact of Roasting on Polyphenols, Caffeine, and Acrylamide in Specialty Coffee Beans

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1310
Author(s):  
Matúš Várady ◽  
Sylwester Ślusarczyk ◽  
Jana Boržíkova ◽  
Katarína Hanková ◽  
Michaela Vieriková ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Dry Matter ◽  
Green Beans ◽  
Caffeoylquinic Acids ◽  
Coffee Beans ◽  
Metal Contents ◽  
Specialty Coffee ◽  
Quantitative Analyses ◽  
The Impact

The aim of this study was to determine the effect of roasting on the contents of polyphenols (PPH), acrylamide (AA), and caffeine (CAF) and to analyze heavy metals in specialty coffee beans from Colombia (COL) and Nicaragua (NIC). Samples of NIC were naturally processed and COL was fermented anaerobically. Green beans from COL (COL-GR) and NIC (NIC-GR) were roasted at two levels, light roasting (COL-LIGHT and NIC-LIGHT) and darker roasting (COL-DARK and NIC-DARK), at final temperatures of 210 °C (10 min) and 215 °C (12 min), respectively. Quantitative analyses of PPH identified caffeoylquinic acids (CQA), feruloylquinic acids, and dicaffeoylquinic acids. Isomer 5-CQA was present at the highest levels and reached 60.8 and 57.7% in COL-GR and NIC-GR, 23.4 and 29.3% in COL-LIGHT and NIC-LIGHT, and 18 and 24.2% in COL-DARK and NIC-DARK, respectively, of the total PPH. The total PPH contents were highest in COL-GR (59.76 mg/g dry matter, DM). Roasting affected the contents of PPH, CAF, and AA (p < 0.001, p < 0.011 and p < 0.001, respectively). Nickel and cadmium contents were significantly higher in the COL-GR than in the NIC-GR beans. Darker roasting decreased AA content, but light roasting maintained similar amounts of CAF and total PPH.

scholarly journals Successive Harvests Modulate the Productive and Physiological Behavior of Three Genovese Pesto Basil Cultivars

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 560
Author(s):  
Luigi Formisano ◽  
Michele Ciriello ◽  
Christophe El-Nakhel ◽  
Marios C. Kyriacou ◽  
Youssef Rouphael
Keyword(s):  
Dry Matter ◽  
Co2 Assimilation ◽  
Stomatal Resistance ◽  
Matter Content ◽  
Dry Matter Content ◽  
Technological Parameters ◽  
Net Co2 Assimilation ◽  
Dry Biomass ◽  
Crop Cycle ◽  
The Impact

In the Italian culinary tradition, young and tender leaves of Genovese basil (Ocimum basilicum L.) are used to prepare pesto sauce, a tasty condiment that attracts the interest of the food processing industry. Like other leafy or aromatic vegetables, basil is harvested more than once during the crop cycle to maximize yield. However, the mechanical stress induced by successive cuts can affect crucial parameters associated with pesto processing (leaf/stem ratio, stem diameter, and dry matter). Our research accordingly aimed to evaluate the impact of successive harvests on three field-grown Genovese basil cultivars (“Aroma 2”, “Eleonora” and “Italiano Classico”) in terms of production, physiological behavior, and technological parameters. Between the first and second harvest, marketable fresh yield and shoot dry biomass increased by 148.4% and 172.9%, respectively; by contrast, the leaf-to-stem ratio decreased by 22.5%, while the dry matter content was unchanged. The increased fresh yield and shoot dry biomass at the second harvest derived from improved photosynthetic efficiency, which enabled higher net CO2 assimilation, Fv/Fm and transpiration as well as reduced stomatal resistance. Our findings suggest that, under the Mediterranean environment, “Italiano Classico” carries superior productive performance and optimal technological characteristics in line with industrial requirements. These promising results warrant further investigation of the impact successive harvests may have on the qualitative components of high-yielding basil genotypes with respect to consumer expectations of the final product.

scholarly journals Effects of voluntary removal of ractopamine hydrochloride (Optaflexx) on live performance and carcass characteristics of beef steers

2021 ◽  
Author(s):  
P J Rincker ◽  
J B Allen ◽  
M Edmonds ◽  
M S Brown ◽  
J C Kube
Keyword(s):  
Dry Matter ◽  
Carcass Characteristics ◽  
Carcass Weight ◽  
Dry Matter Intake ◽  
Beef Steers ◽  
Live Performance ◽  
Meet Residue ◽  
Ractopamine Hydrochloride ◽  
The Impact ◽  
Linear Manner

Abstract There is a lack of consistency across the globe in how countries establish tissue ractopamine residue limits and which residue limits are applied to various tissues, particularly for edible noncarcass tissues. Therefore, some US beef slaughter organizations have recommended a 48-h voluntary removal of ractopamine before slaughter in order to meet residue requirements of specific export countries and maintain international trade. Our objective was to assess the impact of voluntary removal of ractopamine hydrochloride (Optaflexx®; Elanco, Greenfield, IN) up to 8 d before slaughter on growth performance and carcass characteristics. Crossbred beef steers (60 pens of 10 animals/pen) with an initial shrunk body weight (BW) of 611.8 ± 10 kg SEM were fed one of six treatments over 42 d. Treatments included a control that did not receive ractopamine, on-label use of ractopamine (0-d withdrawal), and 2, 4, 6, or 8 d of voluntary removal of ractopamine from feed before slaughter. The start of ractopamine feeding (30.1 mg/kg of diet dry matter for 32 d) was staggered so that blocks could be slaughtered on the same day. Dry matter intake was decreased by 0.5 kg/d when ractopamine was fed with a 0-d withdrawal (P = 0.04) compared to the control, but was not altered (P = 0.56) as the duration of ractopamine removal increased from 0 to 8 d. Final BW, total BW gain, and average daily BW gain were increased by feeding ractopamine with a 0-d withdrawal (P = 0.09) compared to the control, but these variables decreased in a linear manner (P = 0.10) as the duration of removal increased from 0 to 8 d. Gain efficiency was improved by 15% (P &lt; 0.01) by feeding ractopamine with a 0-d withdrawal compared to the control, and gain efficiency decreased linearly (P = 0.06) as the duration of ractopamine removal increased. Approximately 2/3 of the increase in gain efficiency remained after 8 d of removal. Hot carcass weight was increased by 6 kg (P = 0.02) by feeding ractopamine with a 0-d withdrawal compared to the control. Measured carcass characteristics were not altered by ractopamine feeding or by removal before slaughter (P ≥ 0.24). The consequences of voluntary removal of ractopamine up to 8 d before slaughter were a linear decrease in live BW gain (0.64 kg/d), poorer gain efficiency, and numerically lighter carcass weight.

scholarly journals 129 Effects of a multi-strain Bacillus subtilis-based direct-fed microbial on growth performance, nutrient digestibility, and colonic pH in diets fed to weanling pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 101-102
Author(s):  
Jaron R Lewton ◽  
Adrienne Woodward ◽  
Ronny Moser ◽  
Kyan M Thelen ◽  
Adam J Moeser ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Distal Colon ◽  
Nutrient Digestibility ◽  
Nursery Pigs ◽  
Fiber Digestion ◽  
Males And Females ◽  
Dietary Treatments ◽  
The Impact

Abstract A study was conducted to evaluate the effects of a multi-strain Bacillus subtilis-based direct-fed microbial (DFM) on apparent digestibility and colonic pH of nursery pigs. Eighty pigs, of equal number of barrows and gilts (initial BW: 6.99 ± 1.67 kg), were weaned at 21 ± 1 d and randomly allotted to sixteen pens, with five pigs per pen. Two dietary treatments were implemented, a basal control (CON) and a control plus DFM (DFM). Both diets were corn, soybean meal, and distillers dried grains based, formulated to meet all or exceed all nutritional requirements, and manufactured on site. Diets were fed for 42 days. Performance measures were recorded weekly. On d 21 and 42 of the experiment, one pig per pen was randomly selected and euthanized, with equal number of males and females represented. Digestibility of specific nutrients was evaluated within the duodenum, jejunum, ileum, ascending and distal colon. There were no overall differences in growth performance. Overall means ± SD were 0.51 ± 0.05 kg/d, 0.79 ± 0.05 kg/d and 0.66 ± 0.05 for ADG, ADFI, and G:F, respectively. Digestibility of tryptophan within the jejunum tended (P = 0.06) to increase with addition of DFM, as did cysteine (P = 0.12) and methionine (P = 0.10). The analysis also suggested that the impact of the DFM on the digestibility of amino acids may be early in the nursery phase. The pH of contents in ascending colon, a possible indicator of varied fiber digestion, did not differ. Likewise, no differences were observed between treatment in apparent total tract nitrogen and energy digestibility (analysis of distal colon contents). The addition of a multi-strain Bacillus subtilis-based DFM appears to impact digestibility of select amino acids depending upon location in the gastrointestinal tract.

scholarly journals Lawsonia intracellularis infected enterocytes lack sucrase-isomaltase which contributes to reduced pig digestive capacity

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Emma T. Helm ◽  
Eric R. Burrough ◽  
Fernando L. Leite ◽  
Nicholas K. Gabler
Keyword(s):  
Dry Matter ◽  
Post Inoculation ◽  
Intestinal Function ◽  
Lawsonia Intracellularis ◽  
Sample Collection ◽  
Treatment Groups ◽  
Swine Herds ◽  
The Impact ◽  
Lesion Severity

AbstractLawsonia intracellularis is endemic to swine herds worldwide, however much is still unknown regarding its impact on intestinal function. Thus, this study aimed to characterize the impact of L. intracellularis on digestive function, and how vaccination mitigates these impacts. Thirty-six L. intracellularis negative barrows were assigned to treatment groups (n  =  12/trt): (1) nonvaccinated, L. intracellularis negative (NC); (2) nonvaccinated, L intracellularis challenged (PC); and (3) L. intracellularis challenged, vaccinated (Enterisol® Ileitis, Boehringer Ingelheim) 7 weeks pre-challenge (VAC). On days post-inoculation (dpi) 0 PC and VAC pigs were inoculated with L. intracellularis. From dpi 19–21 fecal samples were collected for apparent total tract digestibility (ATTD) and at dpi 21, pigs were euthanized for sample collection. Post-inoculation, ADG was reduced in PC pigs compared with NC (41%, P  <  0.001) and VAC (25%, P  <  0.001) pigs. Ileal gross lesion severity was greater in PC pigs compared with NC (P  =  0.003) and VAC (P  =  0.018) pigs. Dry matter, organic matter, nitrogen, and energy ATTD were reduced in PC pigs compared with NC pigs (P  ≤  0.001 for all). RNAscope in situ hybridization revealed abolition of sucrase-isomaltase transcript in the ileum of PC pigs compared with NC and VAC pigs (P  <  0.01). Conversely, abundance of stem cell signaling markers Wnt3, Hes1, and p27Kip1 were increased in PC pigs compared with NC pigs (P  ≤  0.085). Taken together, these data demonstrate that reduced digestibility during L. intracellularis challenge is partially driven by abolition of digestive machinery in lesioned tissue. Further, vaccination mitigated several of these effects, likely from lower bacterial burden and reduced disease severity.

scholarly journals Impact of a phytogenic feed additive on growth performance, feed intake, and carcass traits of finishing steers

2019 ◽  
Vol 3 (4) ◽  
pp. 1162-1172
Author(s):  
Tassilo Brand ◽  
Martin Hünerberg ◽  
Tim A McAllister ◽  
Maolong He ◽  
Atef M Saleem ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Intake ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Carcass Traits ◽  
Feed Additive ◽  
Muscle Area ◽  
Mineral Supplement ◽  
Liver Abscesses ◽  
Finishing Steers

Abstact: The purpose of this study was to evaluate the effect of a phytogenic feed additive (Digestarom [DA]; Biomin, Getzersdorf, Austria) on growth performance, feed intake, carcass traits, fatty acid composition, and liver abscesses of finishing steers. One hundred twenty Angus × Charolais crossbred steers (488 ± 26.5 kg) were used in a 110-d feeding experiment. Steers were blocked by weight and randomly assigned to 12 pens with 10 steers per pen. Each pen was allocated to one of three diets. Each diet contained 86.5% barley, 10.0% barley silage, and 3.5% vitamin and mineral supplement on a dry matter (DM) basis. The diets contained 0, 0.05, and 0.1 g DA/kg complete diet (DM basis), to achieve average daily DA intakes of 0 (control), 0.5 (LowDA), and 1.0 g (HighDA) per steer. Diets were prepared once daily and provided ad libitum. Two pens per treatment were equipped to record individual feed intake behavior. Steers were weighed every 28 d and carcass traits and liver scores were recorded at slaughter. Dry matter intake (average: 9.34 kg/d) did not differ (P &gt; 0.05) among diets. Average daily gain tended to increase linearly as DA increased (control: 1.82; LowDA: 1.87; and HighDA: 1.95 kg/d; P &lt; 0.09), but gain:feed ratio was not affected. Supplementation of DA affected longissimus muscle area quadratically (P = 0.05) with the largest area observed for LowDA. However, dressing percentage decreased linearly in response to increasing level of DA (P &lt; 0.01). Total abscessed livers were not affected, whereas proportion of severe liver abscesses was numerically lower with DA (30.8% and 42.5% for LowDA and HighDA) compared to the control (50%).

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