scholarly journals Bacterial Adhesion of Streptococcus mutans to Dental Material Surfaces

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1152
Author(s):  
Mirjam Kozmos ◽  
Petra Virant ◽  
Franc Rojko ◽  
Anže Abram ◽  
Rebeka Rudolf ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
Surface Characteristics ◽  
Surface Hydrophobicity ◽  
Dental Material ◽  
Average Roughness ◽  
Arithmetic Average ◽  
Streaming Potentials ◽  
Material Surfaces ◽  
Pt Alloy

The aim of this study was to investigate and understand bacterial adhesion to different dental material surfaces like amalgam, Chromasit, an Co-Cr alloy, an IPS InLine ceramic, yttrium stabilized tetragonal polycrystalline zirconia (TPZ), a resin-based composite, an Au-Pt alloy, and a tooth. For all materials, the surface roughness was assessed by profilometry, the surface hydrophobicity was determined by tensiometry, and the zeta potential was measured by electrokinetic phenomena. The arithmetic average roughness was the lowest for the TPZ ceramic (Ra = 0.23 µm ± 0.02 µm), while the highest value was observed for the Au-Pt alloy (Ra = 0.356 µm ± 0.075 µm). The hydrophobicity was the lowest on the TPZ ceramic and the highest on the Co-Cr alloy. All measured streaming potentials were negative. The most important cause of tooth caries is the bacterium Streptococcus mutans, which was chosen for this study. The bacterial adhesion to all material surfaces was determined by scanning electron microscopy. We showed that the lowest bacterial extent was on the amalgam, whereas the greatest extent was on tooth surfaces. In general, measurements showed that surface properties like roughness, hydrophobicity and charge have a significant influence on bacterial adhesion extent. Therefore, dental material development should focus on improving surface characteristics to reduce the risk of secondary caries.

scholarly journals Assessment of Streptococcus Mutans Adhesion to the Surface of Biomimetically-Modified Orthodontic Archwires

Coatings ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 201
Author(s):  
Santiago Arango-Santander ◽  
Carolina Gonzalez ◽  
Anizac Aguilar ◽  
Alejandro Cano ◽  
Sergio Castro ◽  
...  
Keyword(s):  
Contact Angle ◽  
Surface Modification ◽  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
Soft Lithography ◽  
Surface Hydrophobicity ◽  
Colocasia Esculenta ◽  
Oral Diseases ◽  
Pdms Stamp ◽  
Orthodontic Archwires

Bacterial adhesion and biofilm formation on the surfaces of dental and orthodontic biomaterials is primary responsible for oral diseases and biomaterial deterioration. A number of alternatives to reduce bacterial adhesion to biomaterials, including surface modification using a variety of techniques, has been proposed. Even though surface modification has demonstrated a reduction in bacterial adhesion, information on surface modification and biomimetics to reduce bacterial adhesion to a surface is scarce. Therefore, the main objective of this work was to assess bacterial adhesion to orthodontic archwires that were modified following a biomimetic approach. The sample consisted of 0.017 × 0.025, 10 mm-long 316L stainless steel and NiTi orthodontic archwire fragments. For soft lithography, a polydimethylsiloxane (PDMS) stamp was obtained after duplicating the surface of Colocasia esculenta (L) Schott leaves. Topography transfer to the archwires was performed using silica sol. Surface hydrophobicity was assessed by contact angle and surface roughness by atomic force microscopy. Bacterial adhesion was evaluated using Streptococcus mutans. The topography of the Colocasia esculenta (L) Schott leaf was successfully transferred to the surface of the archwires. Contact angle and roughness between modified and unmodified archwire surfaces was statistically significant. A statistically significant reduction in Streptococcus mutans adhesion to modified archwires was also observed.

scholarly journals Influence of Surface Properties on the Adhesion ofStaphylococcus epidermidisto Acrylic and Silicone

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Cláudia Sousa ◽  
Pilar Teixeira ◽  
Rosário Oliveira
Keyword(s):  
Surface Properties ◽  
Staphylococcus Epidermidis ◽  
Bacterial Adhesion ◽  
Surface Characteristics ◽  
Surface Hydrophobicity ◽  
Force Microscopy ◽  
Angle Measurements ◽  
Atomic Force ◽  
Contact Angle Measurements ◽  
Biomaterial Surface

The aim of the present study was to compare the ability of eightStaphylococcus epidermidisstrains to adhere to acrylic and silicone, two polymers normally used in medical devices manufacture. Furthermore, it was tried to correlate that with the surface properties of substrata and cells. Therefore, hydrophobicity and surface tension components were calculated through contact angle measurements. Surface roughness of substrata was also assessed by atomic force microscopy (AFM). No relationship was found between microbial surface hydrophobicity and adhesion capability. Nevertheless,Staphylococcus epidermidisIE214 showed very unique adhesion behaviour, with cells highly aggregated between them, which is a consequence of their specific surface features. All strains, determined as being hydrophilic, adhered at a higher extent to silicone than to acrylic, most likely due to its more hydrophobic character and higher roughness. This demonstrates the importance of biomaterial surface characteristics for bacterial adhesion.

scholarly journals A Concise Review of Silver Diamine Fluoride on Oral Biofilm

2021 ◽  
Vol 11 (7) ◽  
pp. 3232
Author(s):  
Jingyang Zhang ◽  
Sofiya-Roksolana Got ◽  
Iris Xiaoxue Yin ◽  
Edward Chin-Man Lo ◽  
Chun-Hung Chu
Keyword(s):  
Clinical Study ◽  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
Clinical Studies ◽  
Antibacterial Properties ◽  
Tooth Surface ◽  
Original Research ◽  
Laboratory Studies ◽  
Oral Biofilm ◽  
Silver Diamine Fluoride

Studies have shown that silver diamine fluoride (SDF) is an effective agent to arrest and prevent dental caries due to its mineralizing and antibacterial properties. While plenty of studies have investigated the mineralizing properties, there are few papers that have examined its antibacterial effect on oral biofilm. The objective of this study was to identify the effect of silver diamine fluoride on oral biofilm. Method: The keywords used were (silver diamine fluoride OR silver diammine fluoride OR SDF OR silver fluoride OR AgF AND biofilm OR plaque). Two reviewers screened the titles and abstracts and then retrieved the full text of the potentially eligible publications. Publications of original research investigating the effect of SDF on oral biofilm were selected for this review. Results: This review included 15 laboratory studies and six clinical studies among the 540 papers identified. The laboratory studies found that SDF could prevent bacterial adhesion to the tooth surface. SDF also inhibited the growth of cariogenic bacteria, including Streptococcus mutans, Lactobacillus acidophilus, Streptococcus sobrinus, Lactobacillus rhamnosus, Actinomyces naeslundii, and Enterococcus faecalis, thus contributing to its success in caries arrest. One clinical study reported a decrease in Streptococcus mutans and Lactobacillus sp. in arrested caries after SDF treatment, and another clinical study found that SDF inhibited the growth of periodontitis microbiota, including Porphyromonas gingivalis, Tannerella forsythia, and Prevotella intermedia/nigrescens. However, three clinical studies reported no significant change in the microbial diversity of the plaque on the tooth after SDF treatment. Moreover, one laboratory study and one clinical research study reported that SDF inhibited the growth of Candida albicans. Conclusion: Not many research studies have investigated the effects of SDF on oral biofilm, although SDF has been used as a caries-arresting agent with antibacterial properties. However, a few publications have reported that SDF prevented bacterial adhesion to the teeth, inhibited the growth of cariogenic and periodontal bacteria, and possessed antifungal properties.

Effect of Surface State of Die on Flow Rate of Steamed Bamboo Powder in Thermal Flow Test Using Capillary Rheometer

2015 ◽  
Vol 651-653 ◽  
pp. 830-835
Author(s):  
Shohei Kajikawa ◽  
Riku Sakagami ◽  
Takashi Iizuka
Keyword(s):  
Particle Size ◽  
Flow Rate ◽  
Surface State ◽  
Flow Behavior ◽  
Thermal Flow ◽  
Average Roughness ◽  
Arithmetic Average ◽  
Flow Test ◽  
Die Surface ◽  
Bamboo Powder

Thermal flow tests were performed on steamed bamboo powder using capillaries that were processed under different conditions in order to investigate the effect of the die surface state on the fluidity of the woody powder. The capillaries were processed by wire-cut electric discharge machining, reaming or drilling, and the arithmetic average roughness (Ra) varied from 0.5 to 2.5 μm. The bamboo powder was first steamed at 200 °C for 20 min, and its particle size was then controlled using different mesh screens. The thermal flow temperature was set at 200 °C. The results indicated that the flow behavior improved with increasing particle size. For the capillaries processed by WEDM, the flow rate for samples with particle sizes of 75~150 and 150~300 μm decreased with increasing Ra. On the other hand, when reaming or drilling was used to process the capillaries, the flow rate was almost independent of Ra, regardless of the particle size.

In Situ: A Novel Approach for the Production of Micro Holes

2010 ◽  
Vol 126-128 ◽  
pp. 885-890
Author(s):  
K.P. Somashekhar ◽  
N. Ramachandran ◽  
Jose Mathew
Keyword(s):  
Surface Roughness ◽  
Process Parameters ◽  
Feed Rate ◽  
Machining Parameters ◽  
Average Roughness ◽  
Arithmetic Average ◽  
Quantitative Correlation ◽  
Good Surface ◽  
Novel Approach ◽  
Micro Holes

This work is on the preparation of microelectrodes for μ-EDM operation using μ-WEDG process. Electrodes of Ø500 μm are fabricated with various discharge energy machining conditions. Effects of gap voltage, capacitance & feed rate on the surface finish of the electrodes and overcut of the thus produced micro holes are investigated. The profile of microelectrodes is measured using surface roughness tester with 2μm stylus interfaced with SURFPAK software. The study demonstrated that for brass electrodes an arithmetic average roughness value as low as 1.7μm and an overcut of 3 µm could be achieved. The significant machining parameters are found using ANOVA. Surface of the produced microelectrodes are examined using Scanning Electron Microscope. μ-WEDG process parameters could be adjusted to achieve good surface integrity on microelectrodes. Experimental results showed that the surface roughness of microelectrodes depended primarily on feed rate of the electrode. The observations showed the clear and quantitative correlation existing between the micrometer level surface quality and process parameters. The resulting microelectrodes are found to be of exceptionally high quality and could be used for μ- EDM operation on different types of work materials.

Impact of Physical Chemical Characteristics of Abutment Implant Surfaces on Bacteria Adhesion

2016 ◽  
Vol 42 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Erica Dorigatti de Avila ◽  
Rafael Scaf de Molon ◽  
Bruno P. Lima ◽  
Renate Lux ◽  
Wenyuan Shi ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Electrostatic Interactions ◽  
Chemical Elements ◽  
Human Tooth ◽  
Chemical Characteristics ◽  
Material Surfaces ◽  
Initial Adhesion ◽  
Implant Abutment ◽  
Adhesion Phase ◽  
Bacteria Adhesion

Surface attachment is the first step in biofilm formation, and the ability of bacteria to adhere to surfaces and develop a biofilm is directly influenced by electrostatic interactions between the bacteria and the chemical composition of material surfaces. Here, we investigated the influence of physical and chemical characteristics of titanium (Ti) and zirconia (ZrO2) as implant abutment surfaces on the bacterial adhesion phase and compared the results to bovine enamel (BE) simulating a human tooth. To achieve this goal, we used 2 common pathogens of the oral cavity, Streptococcus mutans UA140 and Porphyromonas gingivalis 33277. To investigate the influence of material surfaces on bacterial adhesion, we studied the surface free energy as well as the topography by atomic force microscopy, and the chemical elements composition by scanning electron microscopy equipped with an energy dispersive X-ray spectroscope. Our results indicated a hydrophobic characteristic for all of the materials; however, the presence of polar and nonpolar components could aid in understanding why greater numbers of bacteria had adhered to BE compared to the other surfaces. Our confocal microscopy data support the proposition that electrostatic interactions, indeed, affected the initial adhesion phase. Within the limitations of a laboratory study, the results revealed bacterial adhered on BE and no bacteria could be observed by confocal images on Ti and ZrO2 implant abutment surfaces.

scholarly journals Casein phosphopeptide acts synergistically with fluoride to inhibit initial adhesion of Streptococcus mutans to the saliva-coated hydroxyapatite disc

2020 ◽  
Author(s):  
Xiaodie Wang ◽  
Limin Liu ◽  
Xiaoyan Zhou ◽  
Yongbiao Huo ◽  
Jinlong Gao ◽  
...  
Keyword(s):  
Contact Angle ◽  
Zeta Potential ◽  
Streptococcus Mutans ◽  
Water Contact Angle ◽  
Bacterial Adhesion ◽  
Laser Scanning Microscopy ◽  
Water Contact ◽  
Initial Adhesion ◽  
Salivary Pellicle ◽  
Casein Phosphopeptide

Abstract Background Recent preventive strategies for dental caries focus on targeting the mechanisms underlying biofilm formation, including inhibiting bacterial adhesion. A promising approach to prevent bacterial adhesion is to modify the composition of acquired salivary pellicle. This in vitro study investigated the effect and possible underlying mechanism of pellicle modification by casein phosphopeptide (CPP) on Streptococcus mutans (S. mutans) initial adhesion, and the impact of fluoride on the efficacy of CPP.Methods The salivary pellicle-coated hydroxyapatite (s-HA) disc was modified by 2.5% CPP or 2.5% CPP supplemented with 900 ppm fluoride solutions. After cultivation of S. mutans, the adherent bacteria were visualized by scanning electron microscopy (SEM) and quantitatively evaluated using the plate count method. Confocal laser scanning microscopy (CLSM) was used to evaluate the proportions of total and dead S. mutans. The concentrations of total, free, and bound calcium and fluoride in CPP and fluoride-doped CPP solutions were determined. The water contact angle and zeta potential of s-HA with and without modification were measured. The data were statistically analyzed using one-way ANOVA followed by a Turkey post hoc multiple comparison test.Results Initial adhesion of S. mutans to s-HA was inhibited in response to pellicle modification by CPP and fluoride-doped CPP, and the latter was more efficient. CLSM analysis showed that the proportion of dead S. mutans did not differ between the groups. Water contact angle and zeta potential decreased after pellicle modification, and both were lowest in the fluoride-doped CPP group.Conclusions Pellicle modification by CPP inhibited S. mutans initial adhesion to s-HA, possibly by reducing hydrophobicity and negative charge on the s-HA surface, and incorporating fluoride into CPP enhanced its anti-adhesion effect.

Casein phosphopeptide combined with fluoride enhances the inhibitory effect on initial adhesion of Streptococcus mutans to the saliva-coated hydroxyapatite disc

2020 ◽  
Author(s):  
Xiaodie Wang ◽  
Limin Liu ◽  
Xiaoyan Zhou ◽  
Yongbiao Huo ◽  
Jinlong Gao ◽  
...  
Keyword(s):  
Contact Angle ◽  
Zeta Potential ◽  
Streptococcus Mutans ◽  
Water Contact Angle ◽  
Bacterial Adhesion ◽  
Laser Scanning Microscopy ◽  
Water Contact ◽  
Initial Adhesion ◽  
Salivary Pellicle ◽  
Casein Phosphopeptide

Abstract Background: Recent preventive strategies for dental caries focus on targeting the mechanisms underlying biofilm formation, including inhibiting bacterial adhesion. A promising approach to prevent bacterial adhesion is to modify the composition of acquired salivary pellicle. This in vitro study investigated the effect and possible underlying mechanism of pellicle modification by casein phosphopeptide (CPP) on Streptococcus mutans (S. mutans) initial adhesion, and the impact of fluoride on the efficacy of CPP. Methods: The salivary pellicle-coated hydroxyapatite (s-HA) discs were treated with phosphate buffered saline (blank control), heat-inactivated 2.5% CPP (negative control), 2.5% CPP (CPP) or 2.5% CPP supplemented with 900 ppm fluoride (CPP + F). After cultivation of S. mutans, the adherent bacteria were visualized by scanning electron microscopy (SEM) and quantitatively evaluated using the plate count method. Confocal laser scanning microscopy (CLSM) was used to evaluate the proportions of total and dead S. mutans. The concentrations of total, free, and bound calcium and fluoride in CPP and fluoride-doped CPP solutions were determined. The water contact angle and zeta potential of s-HA with and without modification were measured. The data were statistically analyzed using one-way ANOVA followed by a Turkey post hoc multiple comparison test.Results: Initial adhesion of S. mutans to s-HA was inhibited in response to pellicle modification by CPP and fluoride-doped CPP, and the latter was more efficient. CLSM analysis showed that the proportion of dead S. mutans did not differ between the groups. Water contact angle and zeta potential decreased after pellicle modification, and both were lowest in the CPP + F group. Conclusions: Pellicle modification by CPP inhibited S. mutans initial adhesion to s-HA, possibly by reducing hydrophobicity and negative charge on the s-HA surface, and incorporating fluoride into CPP enhanced its anti-adhesion effect.

Identification of Surface Quality of Plastic Electrodes after Blasting

2013 ◽  
Vol 334-335 ◽  
pp. 71-76
Author(s):  
B. Haluzíková ◽  
Jan Valíček ◽  
P. Škubala ◽  
Marta Harničárová ◽  
D. Bražina ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Surface Roughness ◽  
Surface Characteristics ◽  
Injection Molding Process ◽  
Maximum Height ◽  
Molding Process ◽  
Arithmetic Average ◽  
Average Maximum ◽  
Wide Range

Nowadays, plastics have become one of the most demanded materials, replacing the traditional ones such as metals. Therefore, many companies are concerned with the production of plastics, with their distribution and innovation development. Plastics have found utility in a wide range of applications, we use them every day. Measurement of surface roughness of plastic moldings produced by the injection molding process was carried out by a contact profilometer Mitutoyo Surftest SJ401. A reason for this measurement is to obtain information about surface roughness. For further technical adjustment is required to have higher surface roughness what helps to increase electrical conductivity of plastic moldings. This involves determination of a ratio between Ra/Rz (the ratio between the arithmetic average of the roughness profile Ra and the average maximum height of the profile Rz) in order to satisfy customer demand for achieving better surface characteristics leading to an increase in electrical conductivity.

Self-Affine Measurements on the Fracture Surface of Plastic Materials by AFM

1999 ◽  
Vol 578 ◽  
Author(s):  
E. Reyes ◽  
C. Guerrero ◽  
V. González ◽  
M. Hinojosa
Keyword(s):  
Plastic Material ◽  
Polymeric Materials ◽  
Operating Conditions ◽  
Average Roughness ◽  
Sem Images ◽  
Fracture Surfaces ◽  
Material Surfaces ◽  
Plastic Materials ◽  
Roughness Exponent ◽  
Good Agreement

AbstractThe self-aff'me behavior of fracture surfaces of polymeric materials was qualitatively and quantitatively studied. SEM images of fracture surfaces of both polypropylene and polystyrene show Chevron marks at several magnifications. In addition, for polystyrene the mirror and Hackle zones were also observed. For quantitative analysis, the average roughness exponent, ζ, of height profiles generated by AFM images, was estimated by applying the variable bandwidth method. Values of ζ=0.788 and ζ=0.810 were obtained for polypropylene and polystyrene, respectively. These results are in very good agreement with the claimed universal exponent of 0.8 reported in the literature for other non-polymeric materials. By choosing the AFM appropriate operating conditions, measurements of roughness on plastic material surfaces could be performed.

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