scholarly journals Sterilization Procedure for Temperature-Sensitive Hydrogels Loaded with Silver Nanoparticles for Clinical Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 380 ◽  
Author(s):  
Diana Rafael ◽  
Fernanda Andrade ◽  
Francesc Martinez-Trucharte ◽  
Jana Basas ◽  
Joaquín Seras-Franzoso ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biomedical Applications ◽  
Gelation Time ◽  
Antimicrobial Properties ◽  
High Sensitivity ◽  
Cost Effective ◽  
Raw Material ◽  
Temperature Sensitive ◽  
Dry Heat

Hydrogels (HG) have recognized benefits as drug delivery platforms for biomedical applications. Their high sensitivity to sterilization processes is however one of the greatest challenges regarding their clinical translation. Concerning infection diseases, prevention of post-operatory related infections is crucial to ensure appropriate patient recovery and good clinical outcomes. Silver nanoparticles (AgNPs) have shown good antimicrobial properties but sustained release at the right place is required. Thus, we produced and characterized thermo-sensitive HG based on Pluronic® F127 loaded with AgNPs (HG-AgNPs) and their integrity and functionality after sterilization by dry-heat and autoclave methods were carefully assessed. The quality attributes of HG-AgNPs were seriously affected by dry-heat methods but not by autoclaving methods, which allowed to ensure the required sterility. Also, direct sterilization of the final HG-AgNPs product proved more effective than of the raw material, allowing simpler production procedures in non-sterile conditions. The mechanical properties were assessed in post mortem rat models and the HG-AgNPs were tested for its antimicrobial properties in vitro using extremely drug-resistant (XDR) clinical strains. The produced HG-AgNPs prove to be versatile, easy produced and cost-effective products, with activity against XDR strains and an adequate gelation time and spreadability features and optimal for in situ biomedical applications.

scholarly journals Role of Green Route Synthesized Silver Nanoparticles in Medicinal Applications with Special Reference to Cancer Therapy

2018 ◽  
Vol 15 (4) ◽  
pp. 783-790 ◽  
Author(s):  
S. Rizwana Begum ◽  
D. Muralidhara Rao ◽  
P. Dinesh Sankar Reddy
Keyword(s):  
Silver Nanoparticles ◽  
Cancer Therapy ◽  
Medicinal Plants ◽  
Antimicrobial Properties ◽  
Cost Effective ◽  
Health Issues ◽  
Promising Tool ◽  
Green Route

Nanotechnology is a blazing field for the researchers in modern branch of science along with engineering have lot of applications. Nanotechnology is an imminent field with new outlet to fight and prevent many diseases using nanoparticles. Among the most promising materials Silver nanoparticles are having antimicrobial properties which are synthesized from medicinal plant and acts against chronic diseases. Silver nanoparticles synthesized from medicinal plants have lot of applications and eco-friendly, cost effective in nature. The present review article mainly focuses on biologically synthesized silver nanoparticles from medicinal plants and its role on cancer cells. Cancer is one of the most difficult health issues on globe. Although number of treatments may include radiation, chemotherapy and surgery, but these procedures not only targets tumor tissue but also normal healthy tissue. In recent years silver nanoparticles are considered as promising tool for cancer therapy. A numerous studies both in-vitro and in-vivo suggested that sliver nanoparticles can be used as cytotoxic and genotoxic agent due to their apoptotic inducing and anti-proliferative properties. However there is need to overlook the mechanism regarding the anti-cancerous activity. A silver nanoparticle deploys in every field of engineering science and medical sciences are still attracting to explore new scope of nanobiotechnology attributed with smaller size particles.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

scholarly journals Silver Nanoparticles Protect Skin from Ultraviolet B-Induced Damage in Mice

2020 ◽  
Vol 21 (19) ◽  
pp. 7082
Author(s):  
Yu-Yi Ho ◽  
Der-Shan Sun ◽  
Hsin-Hou Chang
Keyword(s):  
Silver Nanoparticles ◽  
Biomedical Applications ◽  
Animal Studies ◽  
Wide Spectrum ◽  
Ultraviolet B ◽  
Skin Damage ◽  
Effective Protection ◽  
Uv Illumination ◽  
Nanosized Titanium Dioxide

Ultraviolet (UV) radiation from sunlight has various adverse effects; thus, UV blockage is recommended for preventing sunburn. Common sunscreen ingredients, such as nanosized titanium dioxide and zinc oxide, offer effective protection and enhance cosmetic appearance; however, health concerns have been raised regarding their photocatalytic activity, which generates reactive oxygen species under UV illumination. Silver nanoparticles (AgNPs) are known as safe materials for use in a wide spectrum of biomedical applications. In vitro studies have revealed that AgNPs may have a protective effect against UV irradiation, but the effects in animal studies remain unclear. The present study demonstrated that AgNPs effectively protect against UVB-induced skin damage both in cell cultures and mouse models. These results suggested that AgNPs are feasible and safe as sunscreen ingredients for protection against UVB-induced skin damage.

Synthesis of Silver Nanoparticles and their Biomedical Applications - A Comprehensive Review

2019 ◽  
Vol 25 (24) ◽  
pp. 2650-2660 ◽  
Author(s):  
Rajasree Shanmuganathan ◽  
Indira Karuppusamy ◽  
Muthupandian Saravanan ◽  
Harshiny Muthukumar ◽  
Kumar Ponnuchamy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biomedical Applications ◽  
Antibacterial Agents ◽  
Cost Effective ◽  
Metallic Silver ◽  
Synthesis Methods ◽  
Burn Wound ◽  
Chemical Methods ◽  
Biological Sources ◽  
Physical And Chemical

Generally, silver is considered as a noble metal used for treating burn wound infections, open wounds and cuts. However, the emerging nanotechnology has made a remarkable impact by converting metallic silver into silver nanoparticles (AgNPs) for better applications. The advancement in technology has improved the synthesis of NPs using biological method instead of physical and chemical methods. Nonetheless, synthesizing AgNPs using biological sources is ecofriendly and cost effective. Till date, AgNPs are widely used as antibacterial agents; therefore, a novel idea is needed for the successful use of AgNPs as therapeutic agents to uncertain diseases and infections. In biomedicine, AgNPs possess significant advantages due to their physical and chemical versatility. Indeed, the toxicity concerns regarding AgNPs have created the need for non-toxic and ecofriendly approaches to produce AgNPs. The applications of AgNPs in nanogels, nanosolutions, silver based dressings and coating over medical devices are under progress. Still, an improvised version of AgNPs for extended applications in an ecofriendly manner is the need of the hour. Therefore, the present review emphasizes the synthesis methods, modes of action under dissipative conditions and the various biomedical applications of AgNPs in detail.

scholarly journals Sustained Release from Injectable Composite Gels Loaded with Silver Nanowires Designed to Combat Bacterial Resistance in Bone Regeneration Applications

Pharmaceutics ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 116 ◽  
Author(s):  
Arianna De Mori ◽  
Meena Hafidh ◽  
Natalia Mele ◽  
Rahmi Yusuf ◽  
Guido Cerri ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Composite Scaffold ◽  
Silver Nanowires ◽  
Gelation Time ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Bone Tissue Regeneration ◽  
Composite Gels ◽  
Prevention Of Infections

One-dimensional nanostructures, such as silver nanowires (AgNWs), have attracted considerable attention owing to their outstanding electrical, thermal and antimicrobial properties. However, their application in the prevention of infections linked to bone tissue regeneration intervention has not yet been explored. Here we report on the development of an innovative scaffold prepared from chitosan, composite hydroxyapatite and AgNWs (CS-HACS-AgNWs) having both bioactive and antibacterial properties. In vitro results highlighted the antibacterial potential of AgNWs against both gram-positive and gram-negative bacteria. The CS-HACS-AgNWs composite scaffold demonstrated suitable Ca/P deposition, improved gel strength, reduced gelation time, and sustained Ag+ release within therapeutic concentrations. Antibacterial studies showed that the composite formulation was capable of inhibiting bacterial growth in suspension, and able to completely prevent biofilm formation on the scaffold in the presence of resistant strains. The hydrogels were also shown to be biocompatible, allowing cell proliferation. In summary, the developed CS-HACS-AgNWs composite hydrogels demonstrated significant potential as a scaffold material to be employed in bone regenerative medicine, as they present enhanced mechanical strength combined with the ability to allow calcium salts deposition, while efficiently decreasing the risk of infections. The results presented justify further investigations into the potential clinical applications of these materials.

Graphene Oxide Functionalized with Silver Nanoparticles and ZnO Synergic Nanocomposite as an Efficient Electrochemical Sensor for Diclofenac Sodium

NANO ◽  
2021 ◽  
Author(s):  
Saira Naz ◽  
Amjad Nisar ◽  
Lizhi Qian ◽  
Shafqat Hussain ◽  
Shafqat Karim ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Diclofenac Sodium ◽  
Modified Electrodes ◽  
High Sensitivity ◽  
Cost Effective ◽  
Fast Response ◽  
Industrial Applications ◽  
Ag Nps ◽  
Enhanced Sensitivity

The development of a highly sensitive and selective electrocatalyst for the detection of diclofenac sodium (D.S.) has remained a great challenge. In this work, graphene oxide functionalized with silver nanoparticles and zinc oxide (Ag–ZnO–GO) electrocatalyst was developed and investigated for the detection of D.S. The Ag–ZnO–GO/glassy carbon electrode exhibits high sensitivity and fast response within 3[Formula: see text]s owing to the efficient oxidation of D.S. at a very low potential at 0.25[Formula: see text]V. Moreover, the electrode shows a low detection limit of 0.02[Formula: see text][Formula: see text]M ([Formula: see text]) and long-term stability. To explore the synergic effects, the measurements of D.S. using GO, ZnO and ZnO–GO modified electrodes were also performed. The results demonstrate that the Ag–ZnO–GO nanocomposite electrode exhibits enhanced sensitivity and selectivity compared to the other electrodes. In addition, the electrode reveals excellent results for D.S. detection in the real samples as well. The enhanced performance of the proposed electrode is attributed to the improved electron transfer ability and synergic effects of the plasmonic Ag NPs and ZnO–GO structure. It is expected that Ag–ZnO–GO composite is a promising candidate for the construction of cost-effective electrochemical biosensors for medical and industrial applications.

scholarly journals Composite Scaffolds Based on Silver Nanoparticles for Biomedical Applications

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Jenel Marian Patrascu ◽  
Ioan Avram Nedelcu ◽  
Maria Sonmez ◽  
Denisa Ficai ◽  
Anton Ficai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Composite Materials ◽  
Chemical Reaction ◽  
Biomedical Applications ◽  
Chemical Reduction ◽  
Composite Scaffolds ◽  
Sputtering Deposition ◽  
Plasma Sputtering

This paper presents the synthesis, characterisation, andin vitrotesting of homogenous and heterogeneous materials containing silver nanoparticles (nanoAg). Three types of antiseptic materials based on collagen (COLL), hydroxyapatite (HA), and collagen/hydroxyapatite (COLL/HA) composite materials were obtained. The synthesis of silver nanoparticles was realized by chemical reaction as well as plasma sputtering deposition. The use of chemical reduction allows the synthesis of homogenous materials while the plasma sputtering deposition can be easily used for the synthesis of homogeneous and heterogeneous support. Based on thein vitroassays clear antiseptic activity againstEscherichia coliwas relieved even at low content of nanoAg (10 ppm).

scholarly journals Synergy of Silver Nanoparticles and Aztreonam against Pseudomonas aeruginosa PAO1 Biofilms

2014 ◽  
Vol 58 (10) ◽  
pp. 5818-5830 ◽  
Author(s):  
Marc B. Habash ◽  
Amber J. Park ◽  
Emily C. Vis ◽  
Robert J. Harris ◽  
Cezar M. Khursigara
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Cell Envelope ◽  
Antimicrobial Properties ◽  
Planktonic Cell ◽  
Chronic Infections ◽  
Content Type

ABSTRACTPathogenic bacterial biofilms, such as those found in the lungs of patients with cystic fibrosis (CF), exhibit increased antimicrobial resistance, due in part to the inherent architecture of the biofilm community. The protection provided by the biofilm limits antimicrobial dispersion and penetration and reduces the efficacy of antibiotics that normally inhibit planktonic cell growth. Thus, alternative antimicrobial strategies are required to combat persistent infections. The antimicrobial properties of silver have been known for decades, but silver and silver-containing compounds have recently seen renewed interest as antimicrobial agents for treating bacterial infections. The goal of this study was to assess the efficacy of citrate-capped silver nanoparticles (AgNPs) of various sizes, alone and in combination with the monobactam antibiotic aztreonam, to inhibitPseudomonas aeruginosaPAO1 biofilms. Among the different sizes of AgNPs examined, 10-nm nanoparticles were most effective in inhibiting the recovery ofP. aeruginosabiofilm cultures and showed synergy of inhibition when combined with sub-MIC levels of aztreonam. Visualization of biofilms treated with combinations of 10-nm AgNPs and aztreonam indicated that the synergistic bactericidal effects are likely caused by better penetration of the small AgNPs into the biofilm matrix, which enhances the deleterious effects of aztreonam against the cell envelope ofP. aeruginosawithin the biofilms. These data suggest that small AgNPs synergistically enhance the antimicrobial effects of aztreonam againstP. aeruginosain vitro, and they reveal a potential role for combinations of small AgNPs and antibiotics in treating patients with chronic infections.

scholarly journals PSIDIUM GUAJAVA: A NOVEL PLANT IN THE SYNTHESIS OF SILVER NANOPARTICLES FOR BIOMEDICAL APPLICATIONS.

2018 ◽  
Vol 11 (1) ◽  
pp. 341
Author(s):  
Sharmila C ◽  
Ranjith Kumar R ◽  
Chandar Shekar B
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Biomedical Applications ◽  
Leaf Extract ◽  
Cost Effective ◽  
Psidium Guajava ◽  
Tem Analysis ◽  
X Ray ◽  
Green Route

 Objective: Synthesis of silver nanoparticles (AgNPs) using a simple, cost-effective and environmentally friendly green route approach and to study the antibacterial activity of AgNPs against human pathogens.Methods: Green route approach is used to synthesize AgNPs using Psidium guajava leaf extract. Fourier transform infrared (FTIR) was used to identify the presence of the functional group. X-ray diffraction (XRD) was used to analyze the structure of prepared AgNPs. Energy dispersive X-ray was used to the characteristic to the composition of the prepared nanoparticles. Size and morphology of the prepared AgNPs were investigated using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) analysis. Antibacterials efficiency of prepared AgNPs was tested against Escherichia coli and Staphylococcus aureus by well diffusion methods.Results: FTIR study shows the presence of different functional groups present in the leaves mediated AgNPs. The XRD studies yield diffraction peaks corresponding to face-centered cubic structure of Ag crystals. Spherical shaped AgNPs with a particle size of about ~55 nm were evidenced using FESEM and TEM analysis. Energy dispersive spectrum of the synthesized AgNPs confirms the presence of silver in the prepared nanoparticles. From UV-VIS analysis it is shown that the absorption band was red-shifted from 430 nm to 456 nm. The prepared AgNPs shows good antibacterial activity against E. coli and S. aureus.Conclusions: P. guajava leaf extract is a potential reducing agent to synthesize AgNPs. The green synthesis approach provides cost-effective and eco-friendly nanoparticles, which could be used in biomedical applications.

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