scholarly journals Combined Water Extracts from Oxidation-Treated Leaves and Branches of Hovenia dulcis Has Anti-Hangover and Liver Protective Effects in Binge Alcohol Intake of Male Mice

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4404
Author(s):  
Jihyun Je ◽  
Miyoung Song ◽  
Ji Hyeong Baek ◽  
Jae Soon Kang ◽  
Hye Jin Chung ◽  
...  
Keyword(s):  
Liver Injury ◽  
Traditional Medicine ◽  
Alcohol Intake ◽  
Protective Effects ◽  
Male Mice ◽  
Protective Properties ◽  
Water Extracts ◽  
Underlying Mechanisms ◽  
Dried Extract ◽  
Hovenia Dulcis

Hovenia dulcis, known as the oriental raisin tree, is used for food supplements and traditional medicine for the liver after alcohol-related symptoms. However, little information exists about the use of its leaves and branches. In this study, we established a method to use the leaves and branches to develop anti-hangover treatment and elucidated the underlying mechanisms. Oxidation-treated leaves (OL) exhibited high antioxidant content comparable to that of the peduncles and showed an anti-hangover effect in male mice. The branch extract (BE) was enriched in the flavonoid catechin, approximately five times more than OL extract. The mixture of OL and BE (OLB) was formulated in a 2:1 ratio with frozen-dried extract weight and was tested for anti-hangover effects and protective properties against binge alcohol-induced liver injury. OLB showed better anti-hangover effect than OL. In addition to this anti-hangover effect, OLB protected the liver from oxidative/nitrosative damage induced by binge alcohol intake.

Dendrobine attenuates isoniazid- and rifampicin-induced liver injury by inhibiting miR-295-5p

2020 ◽  
Vol 39 (12) ◽  
pp. 1671-1680
Author(s):  
R Ci ◽  
K Zhang ◽  
A Zhu ◽  
W Zang
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Luciferase Reporter ◽  
Protective Effects ◽  
Histological Changes ◽  
Stress Markers ◽  
Oxidative Stress Status ◽  
Protein Expressions ◽  
Polymerase Chain ◽  
Underlying Mechanisms

The present study aims to investigate the protective effects of Dendrobine and its underlying mechanisms on liver injury induced by isoniazid (INH) and rifampicin (RIF). A mouse model of liver injury was induced by intragastrically administration of 100 mg/kg INH and 100 mg/kg RIF for 14 days. The mice were intragastrically administrated with Dendrobine (50, 100, and 200 mg/kg) before the administration of INH and RIF. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. Oxidative stress markers including glutathione, superoxide dismutase, and malondialdehyde in the liver were measured and liver histopathological examinations were performed. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were applied to determine the mRNA and protein expressions, respectively. Luciferase reporter assay was used to evaluate the interactions between miR-295-5p and CYP1A2. Dendrobine significantly decreased serum ALT and AST and inhibited the liver index and ameliorated the liver histological changes induced by INH and RIF. Besides, Dendrobine also regulated oxidative stress status in the liver by the regulation of CYP1A2. Moreover, mmu-miR-295-5p was identified to target CYP1A2 and to regulate the expression of CYP1A2. In summary, Dendrobine ameliorated INH and RIF induced mouse liver injury by miR-295-5p-mediated CYP1A2 expression.

scholarly journals The Protective Effects of Water Extracts of Compound Turmeric Recipe on Acute Alcoholism: An Experimental Research Using a Mouse Model

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Xian-ting Liang ◽  
Yan-yan Wang ◽  
Xiao-yu Hu ◽  
Shao-bo Wang
Keyword(s):  
Liver Injury ◽  
Curcuma Longa ◽  
Protective Effects ◽  
Alcoholic Liver Injury ◽  
Therapeutic Drugs ◽  
Tnf Α ◽  
Underlying Mechanisms ◽  
Acute Alcoholism ◽  
Factor Α ◽  
The Brain

Acute alcoholism (AAI) is a common emergency. Currently, there is a lack of preventive and therapeutic drugs with superior safety and efficacy. Curcuma longa, Panax ginseng, Pueraria lobata, Pueraria flower, and Hovenia dulcis Thunb., which are the components of compound turmeric recipe (CTR), are, respectively, used in China as adjuvant therapeutic agents for AAI and alcoholic liver injury, respectively. The purpose of this research was to investigate the effect of traditional compound turmeric recipe in anti-inebriation treatment and to identify its underlying mechanisms. The mice were administered with CTR mixture, and ethanol was subsequently given to mice by gavage. The effects of CTR on the righting reflex, 24-hour survival, drunken behavior, blood ethanol concentration, and pathological changes of liver are depicted. The activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were detected. Besides, the activities of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), cytochrome P450 (P450), superoxide dismutase (SOD), and malondialdehyde (MDA) in the liver and the levels of β-endorphin (β-EP) and leucine enkephalin (LENK) in the brain were also measured. Our results demonstrated that CTR can increase the activities of ADH, ALDH, P450, and SOD and decrease the contents of TNF-α, IL-8, and MDA in the liver. In addition, it can decrease the activities of ALT, AST, and ALP in serum and β-EP and LENK activities in the brain. CTR showed effects on prevention of acute alcoholism, promoting wakefulness, and alleviating alcoholic liver injury, which were likely mediated by the above mechanisms.

scholarly journals IL-22 ameliorates LPS-induced acute liver injury by autophagy activation through ATF4-ATG7 signaling

2020 ◽  
Vol 11 (11) ◽  
Author(s):  
Lujing Shao ◽  
Xi Xiong ◽  
Yucai Zhang ◽  
Huijie Miao ◽  
Yuqian Ren ◽  
...  
Keyword(s):  
Liver Injury ◽  
Inflammatory Responses ◽  
Acute Liver Injury ◽  
Mrna Levels ◽  
Protective Effects ◽  
Activating Transcription Factor 4 ◽  
Pre Treatment ◽  
Underlying Mechanisms

Abstract Uncontrollable inflammatory response acts as a driver of sepsis-associated liver injury (SALI). IL-22 plays an important role in regulating inflammatory responses, but its role in SALI remains unknown. The aim of the study was to assess the association of serum IL-22 with SALI in pediatric patients and to enclose the underlying mechanisms of IL-22 involved in lipopolysaccharide (LPS) - induced acute liver injury (ALI) in mice. Serum IL-22 levels in patients with SALI were significantly lower than in septic patients without liver injury, and the area under receiver operating characteristic (ROC) curve of IL-22 for discriminating SALI was 0.765 (95% CI: 0.593–0.937). Pre-administration of recombinant murine IL-22 alleviated LPS-induced ALI in mice, and serum IL-6 levels and the mRNA levels of TNF-α, IL-1β, and IL-6 in livers were decreased in response to IL-22 pre-treatment in mice. More importantly, IL-22 pre-treatment activated hepatic autophagy mediated by activating transcription factor 4 (ATF4)-autophagy-related gene 7 (ATG7) signaling in vivo and in vitro in response to LPS administration. Moreover, knockdown of ATF4 in mice aggravated LPS-induced ALI, which was associated with suppressed ATG7-related autophagy. In addition, the protective effects of IL-22 on LPS-induced ALI was partially blocked by ATF4 knockdown, which was associated with lower expression of LC3II/I in the livers of ATF4 knockdown (HT or Atf4+/−) mice compared with wild-type mice (WT or Atf4+/+) mice. In conclusion, low serum IL-22 level is associated with SALI occurrence, and IL-22 pre-administration activates autophagy in hepatocytes and protects mice against LPS-induced ALI partially related to ATF4-ATG7 signaling pathway.

scholarly journals Lepidium meyenii Walp Exhibits Anti-Inflammatory Activity against ConA-Induced Acute Hepatitis

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Wei Zheng ◽  
Shouwen Du ◽  
Mingyao Tian ◽  
Wang Xu ◽  
Yufei Tian ◽  
...  
Keyword(s):  
Liver Injury ◽  
Acute Hepatitis ◽  
Suppressor Cells ◽  
Protective Effects ◽  
Traditional Herbal Medicine ◽  
Lepidium Meyenii ◽  
Serum Hormone ◽  
Underlying Mechanisms ◽  
Hepatocyte Death ◽  
Killer T Cells

Strong inflammation is a prominent pathogenesis of acute hepatitis, which can induce hepatocyte death and lead to liver failure. Lepidium meyenii Walp (Maca) is a traditional herbal medicine mostly used in improving sperm motility and serum hormone levels, etc. However, there are no reports that showed Maca was designed for treating hepatitis so far. Therefore, the protective effects and pharmacological mechanisms of Maca are unknown in hepatitis. In this study, we found that the protective effects of Maca extract ameliorate ConA-induced acute hepatitis (CIH) and underlying mechanisms. We determined that pretreatment with Maca extract significantly suppressed the production of aminotransferases and inflammatory cytokines, including IFN-γ, TNF-α, IL-1β, IL-2, IL-6, IL-12, and IL-17a, and moderated acute liver injury in CIH. Maca recruited more myeloid-derived suppressor cells (MDSCs) to the liver and suppressed infiltration of natural killer T cells (NKT cells) and macrophages in the liver. Furthermore, our data indicated the molecular mechanism of the inhibitory inflammatory effects of Maca, which should suppress the activation of NF-κB, IFN-γ/STAT1, and IL-6/STAT3 signalings. Collectively, this present research explores Maca as an effective hepatoprotective medicine to inhibit inflammation and liver injury caused by acute hepatitis.

scholarly journals Cardamonin Reduces Acetaminophen-Induced Acute Liver Injury in Mice via Activating Autophagy and NFE2L2 Signaling

2020 ◽  
Vol 11 ◽  
Author(s):  
Qiushi Xu ◽  
Yunhui Fan ◽  
Juan J. Loor ◽  
Yusheng Liang ◽  
Xudong Sun ◽  
...  
Keyword(s):  
Liver Injury ◽  
Acute Liver Injury ◽  
High Mobility ◽  
Hepatoprotective Effect ◽  
Receptor Protein ◽  
Related Factor ◽  
Protective Effects ◽  
Toll Like Receptor 4 ◽  
Sequestosome 1 ◽  
Underlying Mechanisms

Cardamonin (CD), a naturally occurring chalcone derived from the Alpinia species, has been shown to exert antioxidant and anti-inflammatory activity, but its role in the prevention of acetaminophen- (APAP-) induced hepatotoxicity remains elusive. The objective of this study was to determine the protective effects of CD against APAP-induced acute liver injury (ALI) and the underlying mechanisms. Wild-type or transcription factor nuclear factor erythroid 2-related factor 2- (NFE2L2-) deficient mice were treated with CD (50 or 100 mg/kg, i.p.) or vehicle for 24 h. Subsequently, these mice were challenged with APAP (400 mg/kg, i.p.) for 6 h. Liver and blood samples were collected to evaluate liver injury and protein abundance. Treatment with CD significantly reduced APAP-induced hepatotoxicity. Furthermore, CD effectively reduced APAP-induced inflammation by inhibiting high mobility group box 1 (HMGB1), toll-like receptor 4 (TLR4), and NOD-like receptor protein 3 (NLRP3) signaling. In addition, CD induced activation of sequestosome 1 (p62) and NFE2L2 signaling and facilitated autophagy. By applying autophagy inhibitor 3-methyladenine (3-MA; 20 mg/kg, i.p.), further mechanistic exploration revealed that NFE2L2 deficiency promoted autophagic activity induced by CD treatment, which was conducive to the hepatoprotective effect of CD against APAP-induced hepatoxicity in NFE2L2−/− mice. Overall, data suggest that CD has hepatoprotective effect against APAP-induced ALI, which might contribute to the activation of NFE2L2 and autophagy.

Myricetin derived from Hovenia dulcis Thunb. ameliorates vascular endothelial dysfunction and liver injury in high choline-fed mice

2015 ◽  
Vol 6 (5) ◽  
pp. 1620-1634 ◽  
Author(s):  
Jianjun Guo ◽  
Yonghong Meng ◽  
Yan Zhao ◽  
Yuanyuan Hu ◽  
Daoyuan Ren ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Liver Injury ◽  
Vascular Endothelial ◽  
Protective Effects ◽  
Vascular Endothelial Dysfunction ◽  
Dietary Choline ◽  
Hovenia Dulcis

The present study was conducted to explore the protective effects of myricetin (MYR) purified from Hovenia dulcis Thunb. against vascular endothelial dysfunction and liver injury in mice fed with 3% dietary choline water.

The Hepato-protective Effects of Portulaca oleracea L. extract: Review

2019 ◽  
Vol 16 (2) ◽  
pp. 122-126
Author(s):  
Tahereh Farkhondeh ◽  
Saeed Samarghandian ◽  
Mohsen Azimi-Nezhad ◽  
Soroush Hozeifi
Keyword(s):  
Oxidative Stress ◽  
Traditional Medicine ◽  
Hepatic Injury ◽  
Recent Literature ◽  
Portulaca Oleracea ◽  
Google Scholar ◽  
Protective Agent ◽  
Protective Effects ◽  
Lack Of Information ◽  
Underlying Mechanisms

Background: Portulaca oleracea L. (Purslane) has been used in traditional medicine against hepatic injury, although its actual efficacy has not been fully understood. The present study aimed to critically review the recent literature data from 1990 to 2017 regarding the hepato-protective effects of Portulaca oleracea L. and its underlying mechanisms. Methods: Online literature resources were checked using different search engines such as Medline, PubMed, Iran Medex, Scopus, and Google Scholar to identify articles, editorials, and reviews about antidotal effects of Portulaca oleracea L. against hepatotoxic agents. Results: Few studies have indicated that Portulaca oleracea L. shows protective effects against hepatotoxic agents. However, due to lack of information in humans, more studies are needed to confirm the efficacy of Portulaca oleracea L. as a hepato-protective agent. Conclusion: The study found that Portulaca oleracea L. may be effective on hepatotoxicity by modulating oxidative stress and inflammation.

scholarly journals Dietary Beta-Hydroxy Beta-Methyl Butyrate Supplementation Alleviates Liver Injury in Lipopolysaccharide-Challenged Piglets

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Yehui Duan ◽  
Bo Song ◽  
Changbing Zheng ◽  
Yinzhao Zhong ◽  
Qiuping Guo ◽  
...  
Keyword(s):  
Liver Injury ◽  
Mrna Expression ◽  
Basal Diet ◽  
Control Group ◽  
Protective Effects ◽  
Ampk Signaling ◽  
Lps Challenge ◽  
Hepatic Energy Metabolism ◽  
Serum Aspartate Aminotransferase ◽  
Underlying Mechanisms

The current study was performed to investigate whether dietary β-hydroxy-β-methylbutyrate (HMB) could regulate liver injury in a lipopolysaccharide- (LPS-) challenged piglet model and to determine the mechanisms involved. Thirty piglets ( 21 ± 2   days   old , 5.86 ± 0.18   kg body weight) were randomly divided into the control (a basal diet, saline injection), LPS (a basal diet), or LPS+HMB (a basal diet + 0.60% HMB-Ca) group. After 15 d of treatment with LPS and/or HMB, blood and liver samples were obtained. The results showed that in LPS-injected piglets, HMB supplementation ameliorated liver histomorphological abnormalities induced by LPS challenge. Compared to the control group, the activities of serum aspartate aminotransferase and alkaline phosphatase were increased in the LPS-injected piglets ( P < 0.05 ). The LPS challenge also downregulated the mRNA expression of L-PFK, ACO, L-CPT-1, ICDH β, and AMPKα1/2 and upregulated the mRNA expression of PCNA, caspase 3, TNF-α, TLR4, MyD88, NOD1, and NF-κB p65 ( P < 0.05 ). However, these adverse effects of the LPS challenge were reversed by HMB supplementation ( P < 0.05 ). These results indicate that HMB may exert protective effects against LPS-induced liver injury, and the underlying mechanisms might involve the improvement of hepatic energy metabolism via regulating AMPK signaling pathway and the reduction of liver inflammation via modulating TLR4 and NOD signaling pathways.

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