scholarly journals 5-Aminolevulinic Acid and Soil Fertility Enhance the Resistance of Rosemary to Alternaria dauci and Rhizoctonia solani and Modulate Plant Biochemistry

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 585 ◽  
Author(s):  
Hosam O. Elansary ◽  
Diaa O. El-Ansary ◽  
Fahed A. Al-Mana
Keyword(s):  
Rhizoctonia Solani ◽  
Biotic Stress ◽  
Aminolevulinic Acid ◽  
Antioxidant Activities ◽  
Soluble Sugars ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Enzyme Activities ◽  
Gas Exchange Parameters ◽  
Antioxidant Genes ◽  
Alternaria Dauci

Fungal infection of horticultural and cereal crops by Alternaria dauci and Rhizoctonia solani represents an important biotic stress that could be alleviated by application of 5-aminolevulinic acid (ALA) to fertile and poor soils. Therefore, in this study, the morphological, physiological, biochemical, and genetic effects of ALA application (eight weekly applications at 3–10 ppm) to A. dauci- and R. solani-infected Salvia rosmarinus (rosemary) in fertile and poor soils were investigated. ALA-treated plants produced the longest and highest number of branches and had higher fresh and dry weights. There were increases in the major essential oil constituents (1,8-cineole, linalool, camphor, and borneol), as shown by Gas chromatography–mass spectrometry (GC-MS); higher antioxidant activities in DPPH and β-carotene-bleaching assays; upregulated superoxide dismutase (SOD) and catalase (CAT) antioxidant enzyme activities; increased total phenolics, chlorophyll, soluble sugars, and proline; increased gas exchange parameters; enhanced leaf water potential and relative water content (RWC); and upregulated expression of DREB2 and ERF3 (stress-related genes) and FeSOD, Cu/ZnSOD, and MnSOD (antioxidant genes). Several mechanisms were involved, including stress tolerance, antioxidative, and transcription regulation mechanisms. Furthermore, ALA performance was increased in higher-quality soils with higher nutrient content. This study demonstrated the novel application of ALA as a biotic stress ameliorant with enhanced performance in fertile soils.

scholarly journals Tree Bark Phenols Regulate the Physiological and Biochemical Performance of Gladiolus Flowers

Processes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 71 ◽  
Author(s):  
Hosam O. Elansary
Keyword(s):  
Water Content ◽  
Microbial Growth ◽  
Antioxidant Activities ◽  
Soluble Sugars ◽  
Tree Bark ◽  
Flower Senescence ◽  
Bark Extract ◽  
Plant Residues ◽  
Vase Life ◽  
Gas Exchange Parameters

The postharvest physiology of cut flowers is largely dependent on vase life, which is the maximum number of days before flower senescence. The use of tree bark extracts (major forest plant residues), as an eco-friendly and natural antioxidant preservative in holding solutions, is a novel tool for extending flower longevity. The morphological, physiological, biochemical, and genetic responses of Gladiolus grandiflorus cut spikes to Magnolia acuminata and Taxus cuspidata bark extracts as additives in holding solutions were investigated. G. grandiflorus subjected to bark extracts as well as catechin and protocatechuic acid (main phenols) displayed significant increased longevity (up to 18 days), an increased number of open florets, and increased floret fresh weight. Increases in the relative water content, leaf chlorophyll, carotenoids, soluble sugars, and protein content were observed in addition to a reduction in microbial growth in the cut spikes. Gas exchange parameters were higher in the bark extract treatments than in the controls. Higher antioxidant activities were detected and associated with increased superoxide dismutase and catalase enzyme activities and reduced H2O2 accumulation. The bark extract treatments associated with reduced expression of GgCyP1 (produces cysteine protease) and increased expression of both GgDAD1 (defends against apoptotic activity) and GgEXPA1 (regulates petal expansion). Several mechanisms were implicated in these effects, including maintenance of water content, enhanced management of reactive oxygen species (ROS), increased sugar and protein composition, and control of microbial growth. Thus, bark extracts and isolated phenols could be developed as an eco-friendly, non-toxic, and cost-effective natural preservative for cut gladiolus flowers.

scholarly journals 57 Carnosine prevents oxidative damage in myoblast cells derived from porcine skeletal muscle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 59-59
Author(s):  
Marie-France Palin ◽  
Jérôme Lapointe ◽  
Claude Gariépy ◽  
Danièle Beaudry ◽  
Claudia Kalbe
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Biochemical Properties ◽  
Antioxidant Enzyme Activities ◽  
Antioxidant Genes ◽  
Mitochondrial Functions ◽  
Myoblast Cells

Abstract Carnosine (β-alanyl-L-histidine) is a molecule naturally and exclusively present in muscle food with the highest concentrations found in skeletal muscles and brain of the animal. Among its numerous biochemical properties, carnosine has antioxidant activity which include metal ion chelation and free radical scavenging. We have recently reported that high muscle carnosine content in pig is associated with better meat quality. Moreover, supplementing pigs with β-alanine reduced oxidative damage to Longissimus muscle (LM) lipids and proteins. Among previously reported antioxidant activities, carnosine was found to limit the production of reactive oxygen species (ROS) and increase antioxidant enzyme activities. However, these studies were mainly conducted in rodents and cell lines and mechanisms in play remain to be characterized. To determine the effect of carnosine in preventing oxidative damage and characterize the mechanisms in play, we have undertaken experiments using the progeny (myoblasts) of satellite cells isolated from the LM of newborn piglets. Cells were treated with carnosine (0, 10, 25 and 50 mM) for 48 h and were then either collected immediately or treated with H2O2 (0.3 mM, 1 h) to induce an oxidative stress. Our results showed that carnosine prevents oxidative stress through the reduction of total intracellular ROS and by modulating the antioxidant system in myoblasts.Carnosine increased the mRNA abundance of NEF2L2, a transcription factor activated by oxidative stress, and several of its downstream regulated antioxidant genes. Western blot analyses further suggest that the protective effect of carnosine on H2O2-induced oxidative stress is mediated through the p38 MAPK intracellular pathway. Finally, the addition of carnosine to H2O2-treated myoblasts increased the basal cellular oxygen consumption rate (OCR), the ATP-linked OCR and proton leaks, thus suggesting an effect of carnosine on mitochondrial functions. Taken together, these findings demonstrate the important role of carnosine in preventing oxidative damage in porcine muscle cells.

scholarly journals Erratum: Elansary et al., 5-Aminolevulinic Acid and Soil Fertility Enhance the Resistance of Rosemary to Alternaria dauci and Rhizoctonia solani and Modulate Plant Biochemistry. Plants 2019, 8, 585

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 50
Author(s):  
Hosam O. Elansary ◽  
Diaa O. El-Ansary ◽  
Fahed A. Al-Mana
Keyword(s):  
Soil Fertility ◽  
Rhizoctonia Solani ◽  
Aminolevulinic Acid ◽  
Plant Biochemistry ◽  
Alternaria Dauci

The authors wish to make the following corrections to their paper [...]

Composition of essential oil and antioxidant activity of Khat (Catha edulis Forsk), Ethiopia

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Bahir Dar ◽  
Dpph Radical ◽  
Catha Edulis

In this study, we determined the chemical composition and antioxidant activities of the essential oils from two different varieties of khat (Catha edulis Forsk) cultivated in Ethiopia. The essential oils were extracted by hydrodistillation using the Clevenger type apparatus, identifications of compounds were made by gas chromatography and gas chromatography-mass spectrometry (GC-MS). Seventy seven different compounds were identified from essential oils of the two different khat cultivars. The essential oils in the samples from Bahir Dar and Wendo were composed of 50 and 34 compounds, respectively. The major compound identified in khat essentials oils include: limonene, 1-phenyl-1,2-propanedione, 1-hydroxy,1-phenyl-2-propanone, camphor, (sulfurous acid)-2-propylundecyl ester, hexadecane, O-mentha-1(7), 8-dien-3-ol, heptadecane, 10-methylnonadecane, (phthalic acid)-isobutyl octadecyl ester, and tritetracontane. The antioxidant and free radical scavenging activity of the oils were assessed by means of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay. The scavenging activities of the oils were 23.5-23.6 μg AAE/kg of fresh khat sample.

Determination of Antioxidant Activities and Bioactive Components of Foeniculum vulgare Mill. (Samonsabar)

2019 ◽  
Vol 30 (3) ◽  
pp. 16-22
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Scientific Information ◽  
Ethanolic Extract ◽  
The Body ◽  
World Health ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Bioactive Components ◽  
Foeniculum Vulgare

World Health Organization (WHO) estimated that 80% of the population of developing countries use traditional medicines, mostly natural plant products, for their primary health care needs. In the past few decades, the medicinal value of plants has been assumed more important dimension owing largely to the discovery that extracts from plants contain not only primary metabolites but also a diverse array of secondary metabolites with antioxidant potential. Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Antioxidants are vital substances which possess ability to protect the body from damage due to free radical-induced oxidative stress. The purpose of current study was to determine the antioxidant activities and bioactive components of Foeniculum vulgare (fennel) (Samonsabar) seeds by using UV Visible Spectrophotometer (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Aqueous extract of fennel seeds showed more antioxidant activity (IC50: 0.28 ug/ml) than ethanolic extract (IC50: 0.83 ug/ml) and comparable to standard antioxidant, ascorbic acid (IC50: 0.59 ug/ml). GC-MS analysis was fruitful in identification of compounds based on peak area, retention time, molecular formula, molecular weight, MS Fragmentions and pharmacological actions. Ten bioactive phytochemical compounds from aqueous extracts and 11 from ethanolic extract of fennel seeds were identified. These findings indicated that fennel seeds are potential to provide preventive properties against oxidative damage. These results will give scientific information for quality control of indigenous drug to herbal medicine users and local practitioners using fennel for different types of ailments

scholarly journals Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 357
Author(s):  
Tiago A. Conde ◽  
Bruna F. Neves ◽  
Daniela Couto ◽  
Tânia Melo ◽  
Bruno Neves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Fatty Acid Content ◽  
Scenedesmus Obliquus ◽  
Food Sources ◽  
Gas Chromatography Mass Spectrometry ◽  
Nannochloropsis Oceanica ◽  
Omega 3 ◽  
Bioactive Lipids ◽  
Food Ingredients

The demand for sustainable and environmentally friendly food sources and food ingredients is increasing, and microalgae are promoted as a sustainable source of essential and bioactive lipids, with high levels of omega-3 fatty acids (ω-3 FA), comparable to those of fish. However, most FA screening studies on algae are scattered or use different methodologies, preventing a true comparison of its content between microalgae. In this work, we used gas-chromatography mass-spectrometry (GC-MS) to characterize the FA profile of seven different commercial microalgae with biotechnological applications (Chlorella vulgaris, Chlorococcum amblystomatis, Scenedesmus obliquus, Tetraselmis chui, Phaeodactylum tricornutum, Spirulina sp., and Nannochloropsis oceanica). Screening for antioxidant activity was also performed to understand the relationship between FA profile and bioactivity. Microalgae exhibited specific FA profiles with a different composition, namely in the ω-3 FA profile, but with species of the same phylum showing similar tendencies. The different lipid extracts showed similar antioxidant activities, but with a low activity of the extracts of Nannochloropsis oceanica. Overall, this study provides a direct comparison of FA profiles between microalgae species, supporting the role of these species as alternative, sustainable, and healthy sources of essential lipids.

scholarly journals Ducrosia ismaelis Asch. essential oil: chemical composition profile and anticancer, antimicrobial and antioxidant potential assessment

2020 ◽  
Vol 18 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Ramzi A. Mothana ◽  
Fahd A. Nasr ◽  
Jamal M. Khaled ◽  
Omar M. Noman ◽  
Nael Abutaha ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Moderate Activity ◽  
Antioxidant Agent ◽  
Total Oil ◽  
Β Carotene ◽  
Mcf 7

AbstractThe essential oil of Ducrosia ismaelis Asch. (Apiaceae) that grows wild in Saudi Arabia was investigated utilizing gas chromatography (GC), and gas chromatography-mass spectrometry. Fifty constituents were characterized, representing 96.1% of the total oil. The D. ismaelis essential oil (DIEO) was distinguished by a high composition of oxygenated monoterpenes (51.6%). Decanal (40.6%), α-pinene (15.1%) and dodecanal (13.7%) were the fundamental components. Additionally, DIEO was evaluated for its cytotoxic, antibacterial, antifungal and antioxidant activities. DIEO revealed a great cytotoxic effectiveness against the tested cancer cell lines with IC50 values between 66.2 and 137.3 μg/mL particularly against MCF-7 cancer cells. Furthermore, the induction of apoptosis against MCF-7 cells has been asserted using staining assay (annexin VFITC and/or propidium iodide (PI) dyes) and flow cytometry technique. The DIEO possessed a strong antimicrobial activity against Gram-positive bacterial and fungal strains with MIC-values between 0.07 and 0.31 mg/ml. The values of MBC or MFC were almost once higher than those of MIC’s. Moreover, the β-carotene-bleaching and DPPH free radical-scavenging tests showed that DIEO had a moderate activity (68%) as an antioxidant agent in decolouring of the β-carotene at 1.0 mg/mL and a moderate radical scavenging for DPPH (66 and 72%) at 0.50 and 1.0 mg/mL.

scholarly journals Semen quality, lipid peroxidation, and seminal plasma antioxidant status in horses with different intensities of physical exercise

2013 ◽  
Vol 82 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Helena Härtlová ◽  
Radko Rajmon ◽  
Iva Krontorádová ◽  
Jiří Mamica ◽  
Lukáš Zita ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Seminal Plasma ◽  
Aspartate Aminotransferase ◽  
Semen Quality ◽  
Antioxidant Activities ◽  
Membrane Damage ◽  
Antioxidant Enzyme Activities ◽  
Reference Ranges ◽  
Stress Symptoms ◽  
Plasma Antioxidant

The aim of this study was to compare markers of semen quality, sperm membrane damage, and the seminal plasma antioxidant activity in warmblood stallions with and without sport workload stress. Four stallions were used for breeding only (control) and four both for breeding and competition in jumping. Semen samples were collected at 14-day intervals (from June to August) from each stallion (5 ejaculates per stallion). Immediately after sperm collection, a conventional examination of the ejaculate was processed. Catalytic activities of enzymes aspartate aminotransferase, alanin aminotransferase, glutathione peroxidase, superoxide dismutase and indicator of lipoperoxidation - F2α isoprostanes were measured in samples of seminal plasma. Contrary to basic semen quality indicators, the values of seminal plasma pH, aspartate aminotransferase and alanin aminotransferase were significantly (P < 0.05) impaired in the physically stressed stallions. Also, the level of F2α isoprostanes and the activity of superoxide dismutase were significantly (P < 0.05) increased by stress. The antioxidant activities of superoxide dismutase and glutathion peroxidase increased during the monitored period and reflected changes in F2α isoprostane concentration. We can conclude that even the conventional basic sperm indicators stay within the reference ranges of the biochemical indicators of seminal plasma such as pH or AST/ALT activity may be negatively influenced by sport workload stress. Increased concentrations of F2α isoprostanes indicate that lipoperoxidation can be a mechanism of cell membrane destabilization, which is counteracted by an increase of antioxidant enzyme activities. This is the first report of oxidative stress symptoms in normospermic equine semen in relation to stallion sport workload.

scholarly journals Phytochemical, cytotoxicity and antioxidant studies on the stem bark of Piper arborescens

2017 ◽  
Vol 13 (4) ◽  
pp. 840-845 ◽  
Author(s):  
Nicholas Anak Daniel ◽  
Fasihuddin Badruddin Ahmad ◽  
Zaini Assim ◽  
Chua Hun Pin
Keyword(s):  
Brine Shrimp ◽  
Antioxidant Activities ◽  
Stem Bark ◽  
Artemia Salina ◽  
Methyl Eugenol ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Phytochemical Study ◽  
Crude Extracts ◽  
High Cytotoxicity

Phytochemical study on the stem bark of P. arborescens has isolated six secondary metabolites from the crude extracts by using column chromatography, while identification was performed by using Gas Chromatography - Mass Spectrometry (GC-MS), Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared (FTIR). The isolated metabolites were identified as caryophyllene oxide (1), α-bisabolol (2), benzamide 2-(methylamino) (3), 2-ethylpiperidine (4), piperine (5) and methyl eugenol (6). Toxicity test on the four crude extracts of P. arborescens shows a high cytotoxicity against Artemia salina brine shrimp with LC50 values ranging from 13.12 to 58.70 µg/mL. Greater cytotoxicity of the crude extracts of P. arborescens indicated the presence of potent cytotoxic components in this Piper spp. Antioxidant assay of P. arborescens against 2-diphenyl-1-picrylhydrazyl (DPPH) indicated moderate antioxidant activities of methanol, dichloromethane, chloroform and hexane crude extracts with EC50 values of 21.68, 23.82, 32.88 and 36.88 µg/mL, respectively.

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