scholarly journals Plant Growth Inhibitory Activities and Volatile Active Compounds of 53 Spices and Herbs

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 264 ◽  
Author(s):  
Takayuki Sekine ◽  
Kwame Sarpong Appiah ◽  
Majid Azizi ◽  
Yoshiharu Fujii
Keyword(s):  
Growth Inhibition ◽  
Total Activity ◽  
Inhibitory Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Thymus Vulgaris ◽  
Anethum Graveolens ◽  
Hypocotyl Growth ◽  
Radicle Growth ◽  
Active Compounds ◽  
Inhibitory Activities

The inhibitory activities of the leachates and volatiles from 53 plant species (spices and herbs) were evaluated against lettuce (Lactuca sativa “Great Lakes 366”) seedling growth using the sandwich and dish pack methods, respectively. With the sandwich method, parsley (Petroselinum sativum) showed the strongest inhibitory effect on lettuce radicle growth (77%), followed by tarragon (Artemisia dracunculus) (72%). However, caraway (Carum carvi), dill (Anethum graveolens) (seed), laurel (Laurus nobilis), rosemary (Rosmarinus officinalis), and sage (Salvia officinalis) were the most inhibitory species (100% inhibition of lettuce radicle and hypocotyl growth inhibition at all distance wells) in the dish pack method. Cardamom (Elettaria cardamomum) and thyme (Thymus vulgaris) also showed strong inhibitory activity (100% for radicle and hypocotyl growth inhibition at all 41 and 58 mm distance wells). The headspace sampling and gas chromatography-mass spectrometry (GC-MS) analysis identified the main inhibitory active compounds as carvone in caraway and dill (seeds), 1,8-cineole in laurel and cardamom, and borneol in thyme. Both camphor and 1,8-cineole were detected in rosemary and sage, and the total activity evaluation showed that camphor was the major inhibitory compound in rosemary, although both compounds played equal roles in sage.

scholarly journals Caffeine: The Allelochemical Responsible for the Plant Growth Inhibitory Activity of Vietnamese Tea (Camellia sinensis L. Kuntze)

Agronomy ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 396 ◽  
Author(s):  
Van Thi Thanh PHAM ◽  
Tamer ISMAIL ◽  
Maryia MISHYNA ◽  
Kwame Sarpong APPIAH ◽  
Yosei OIKAWA ◽  
...  
Keyword(s):  
Green Tea ◽  
Weed Management ◽  
Inhibitory Activity ◽  
High Performance ◽  
Specific Activity ◽  
Total Activity ◽  
Inhibitory Effect ◽  
Hypocotyl Growth ◽  
Radicle Growth ◽  
Growth Inhibitory Activity

The present study aimed to examine the phytotoxic potential of seven Vietnamese tea samples based on the specific and total activity of caffeine and tea extracts on test plants. The sandwich method results indicated that the inhibitory effect of tea samples on the radicle and hypocotyl growth of lettuce seedlings was dependent on the concentration and type of tea samples, and also the presence of agar soluble allelochemicals. Among the seven tea samples, the leachates from Vinatea-green tea showed the highest inhibition on the radicle growth of lettuce seedlings with 50% suppression at 0.12 mg dry leaves/mL of agar. Caffeine concentration in tea samples analyzed by high-performance liquid chromatography (HPLC) varied from 20.7 to 38.2 µg/mL of dry leaves. The specific activity (EC50 value) of pure caffeine was 75 µg/mL, and the highest total activity of caffeine estimated in Vinatea-green tea was 0.51 [no unit]. Caffeine from green and oolong tea may be considered as one of the contributors to the inhibitory activity of the crude extract. Moreover, the phytotoxicity of pure caffeine and aqueous tea extracts was highly selective on the growth of different plant species. The concentration of caffeine detected from tea farm soil ranged from 0.137 to 0.145 µg/g soil. The results indicated that caffeine might be considered as a promising allelochemical from Vietnamese tea and can be a good candidate for weed management.

scholarly journals Study of Allelopathic Interaction of Essential Oils from Medicinal and Aromatic Plants on Seed Germination and Seedling Growth of Lettuce

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 163 ◽  
Author(s):  
Somayeh Mirmostafaee ◽  
Majid Azizi ◽  
Yoshiharu Fujii
Keyword(s):  
Essential Oils ◽  
Growth Inhibitor ◽  
Lethal Effect ◽  
Inhibitory Effect ◽  
Lettuce Seed ◽  
Aromatic Plants ◽  
Hypocotyl Growth ◽  
Medicinal And Aromatic Plants ◽  
Radicle Growth ◽  
Allelopathic Interaction

Medicinal and aromatic plants have the ability to transmit volatile allelochemicals and affect their surrounding organisms. In this regard, their interaction should also be considered. The inhibitory effects of 112 essential oils on lettuce seed and seedling were investigated by cotton swab method. Germination (G%), Mean germination time (MGT), Lethal of embryo (L%), dormancy (D%), radicle growth (R%), and hypocotyl growth (H%) were measured. Two methods were used for evaluating allelopathic interaction effects: the simplified modified dilution check-board technique (SMCT) and the isobologram. Thymus daenensis had the highest inhibitory effect on G% (IC50 = 2.9 ppm) and the most lethal effect on the embryo (LC50 = 7.2 ppm). Thymus transcaspicus, Dracocephalum moldavica, Artemisia sieberi and Amomum subulatum had the greatest effect on MGT. Ziziphora tenuior, Trachyspermum ammi and Pelargonium graveolens had the highest effect on D%. Origanum vulgare was the strongest growth inhibitor. The highest synergistic effect on G% was in A. subulatum + Mentha suaveolens, on H% was related to Perovskia abrotanoides + T. daenensis, and on R% was observed in Artemisia vulgaris + M. suaveolens. The results of this study can lead to identification of new phytotoxic compounds in EOs and control weeds more effectively.

scholarly journals Contribution of phenolic acids and dimethyl sulfone to the allelopathic effect of invasive Tridax procumbens

2020 ◽  
Vol 50 ◽  
Author(s):  
Yusuf Andriana ◽  
Tran Dang Xuan
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Total Activity ◽  
Inhibitory Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Allelopathic Effect ◽  
Invasive Weed ◽  
Dimethyl Sulfone ◽  
Randomized Design ◽  
Tridax Procumbens

ABSTRACT Tridax procumbens is an invasive weed with a strong allelopathic activity. In this study, the contribution of phenolic acids and dimethyl sulfone to the allelopathic effect of T. procumbens was evaluated against Raphanus sativus. Phenolic acids (benzoic, ellagic and ferulic), vanillin and dimethyl sulfone were identified and quantified from the strongest fraction of T. procumbens, in an allelopathic assay by high performance liquid chromatography and gas chromatography - mass spectrometry. The contribution of phenolic acids and dimethyl sulfone to the allelopathic effect of T. procumbens, expressed as a total activity, was evaluated by comparing the IC50 value to the concentration of each allelochemical, in a completely randomized design. The benzoic acid presented the strongest inhibitory effect (115 mg kg-1) and the highest contribution (0.483) to the allelopathic effect of T. procumbens, followed by vanillin, dimethyl sulfone and ferulic acid.

scholarly journals Volatile Transference and Antimicrobial Activity of Cheeses Made with Ewes’ Milk Fortified with Essential Oils

Foods ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 35 ◽  
Author(s):  
Carmen C. Licon ◽  
Armando Moro ◽  
Celia M. Librán ◽  
Ana M. Molina ◽  
Amaya Zalacain ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Inhibitory Effect ◽  
Starter Cultures ◽  
Stir Bar Sorptive Extraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Thymus Vulgaris ◽  
Volatile Fraction ◽  
Penicillium Verrucosum ◽  
Natural Flora

During the last decades, essential oils (EOs) have been proven to be a natural alternative to additives or pasteurization for the prevention of microbial spoilage in several food matrices. In this work, we tested the antimicrobial activity of EOs from Melissa officinalis, Ocimum basilicum, and Thymus vulgaris against three different microorganisms: Escherichia coli, Clostridium tyrobutyricum, and Penicillium verrucosum. Pressed ewes’ cheese made from milk fortified with EOs (250 mg/kg) was used as a model. The carryover effect of each oil was studied by analyzing the volatile fraction of dairy samples along the cheese-making process using headspace stir bar sorptive extraction coupled to gas chromatography/mass spectrometry. Results showed that the EOs contained in T. vulgaris effectively reduced the counts of C. tyrobutyricum and inhibited completely the growth of P. verrucosum without affecting the natural flora present in the cheese. By contrast, the inhibitory effect of M. officinalis against lactic acid bacteria starter cultures rendered this oil unsuitable for this matrix.

scholarly journals The involvement of protein N-glycosylation in growth processes of Linum usitatissimum L.

2020 ◽  
Vol 64 (11) ◽  
pp. 121-126
Author(s):  
Irina A. Larskaya ◽  
◽  
Farit A. Abdrahimov ◽  
Evgenia O. Fedina ◽  
◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Linum Usitatissimum ◽  
Growth And Development ◽  
Normal Growth ◽  
Vital Staining ◽  
Inhibitory Effect ◽  
Hypocotyl Growth ◽  
Linum Usitatissimum L ◽  
Plant Phenotype ◽  
The Status

The effect of tunicamycin, the inhibitor of protein N-glycosylation, on the root and hypocotyl growth of flax seedlings (Linum usitatissimum L.) was studied. It is known that tunicamycin inhibits the first stage of the formation of the oligosaccharide precursor, which is necessary to initiate the synthesis of N-glycoproteins by inhibiting the activity of N-acetylglucosamine phosphotransferase. The blocking by tunicamycin (25 μM) the early stages of N-glycoprotein formation induced growth inhibition in flax seedlings depending on the age of the plants. So one-day-old seedlings, tunicamycin significantly suppressed the growth of hypocotyls and main roots but the inhibitory effect of the antibiotic on the roots was more significant. The tunicamycin effect on the hypocotyl growth began to appear 60 h after using the inhibitor, while the roots completely stopped growth after 30 h. In two-day-old tunicamycin-treated seedlings, hypocotyl elongation proceeded at approximately the same rate as in untreated plants and suppression of root growth was manifested only for 60 h. The vital staining of ER (Endoplasmic Reticulum)-structures of control and tuncamycin-treated one-day-old flax seedlings by using of ER-TrackerTM Green dye revealed the growth inhibition was accompanied by the changes in the ER morphology. These results indicate the accumulation of misfolded proteins in the ER-lumen, due to an interruption in N-glycosylation of proteins. Thus, it is obvious that the process of N-glycosylation is necessary for the normal growth and development of flax seedlings, and the plant phenotype will be determined by a change in the status of N-glycosylation of specific glycoproteins.

scholarly journals Stage-dependent effect of deferoxamine on growth of Plasmodium falciparum in vitro

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1250-1255 ◽  
Author(s):  
S Whitehead ◽  
TE Peto
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Antimalarial Activity ◽  
Clinical Malaria ◽  
Inhibitory Effect ◽  
Parasite Development ◽  
And Control ◽  
Speed Of Onset

Abstract Deferoxamine (DF) has antimalarial activity that can be demonstrated in vitro and in vivo. This study is designed to examine the speed of onset and stage dependency of growth inhibition by DF and to determine whether its antimalarial activity is cytostatic or cytocidal. Growth inhibition was assessed by suppression of hypoxanthine incorporation and differences in morphologic appearance between treated and control parasites. Using synchronized in vitro cultures of Plasmodium falciparum, growth inhibition by DF was detected within a single parasite cycle. Ring and nonpigmented trophozoite stages were sensitive to the inhibitory effect of DF but cytostatic antimalarial activity was suggested by evidence of parasite recovery in later cycles. However, profound growth inhibition, with no evidence of subsequent recovery, occurred when pigmented trophozoites and early schizonts were exposed to DF. At this stage in parasite development, the activity of DF was cytocidal and furthermore, the critical period of exposure may be as short as 6 hours. These observations suggest that iron chelators may have a role in the treatment of clinical malaria.

Chemical Constituents from Chloranthus elatior and Their Inhibitory Effect on Human Dihydroorotate Dehydrogenase

Planta Medica ◽  
2021 ◽  
Author(s):  
Qian Yang ◽  
An Jia ◽  
Xizi Liu ◽  
Shiyi Han ◽  
Siyang Fan
Keyword(s):  
Circular Dichroism ◽  
Chemical Constituents ◽  
Inhibitory Effect ◽  
Mass Spectrometric ◽  
Dihydroorotate Dehydrogenase ◽  
Active Compounds ◽  
Electronic Circular Dichroism ◽  
Aerial Parts ◽  
Ic50 Values ◽  
Circular Dichroïsm

AbstractA new sesquiterpene, chlorantholide G (1), a new sesquiterpene dimer, elatiolactone (2), and 2 new diterpenes, elatiorlabdane B (3) and elatiorlabdane C (4), together with 51 known compounds, were isolated from the aerial parts of Chloranthus elatior. The new structures including their absolute configurations were mainly established by mass spectrometric, NMR, and electronic circular dichroism experiments. All isolated compounds were tested for their anti-hDHODH activity. (4S,6R)-4-hydroxy-6-isopropyl-3-methylcyclohex-2-enone (5) and (4S,5R,9S,10R)-8(17),12,14-labdatrien-18-oic acid (29) were the most active compounds with IC50 values of 18.7 and 30.7 µM, respectively.

scholarly journals In Vitro α-Glucosidase and α-Amylase Inhibitory Activities of Free and Bound Phenolic Extracts from the Bran and Kernel Fractions of Five Sorghum Grain Genotypes

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1301
Author(s):  
Yun Xiong ◽  
Ken Ng ◽  
Pangzhen Zhang ◽  
Robyn Dorothy Warner ◽  
Shuibao Shen ◽  
...  
Keyword(s):  
Digestive System ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Inhibitory Effects ◽  
Phenolic Contents ◽  
Inhibitory Activities ◽  
Sorghum Grain ◽  
Global Health Challenge ◽  
Phenolic Extracts

Diabetes is a global health challenge. Currently, an effective treatment for diabetes is to reduce the postprandial hyperglycaemia by inhibiting the carbohydrate hydrolysing enzymes in the digestive system. In this study, we investigated the in vitro α-glucosidase and α-amylase inhibitory effects of free and bound phenolic extracts, from the bran and kernel fractions of five sorghum grain genotypes. The results showed that the inhibitory effect of sorghum phenolic extracts depended on the phenolic concentration and composition. Sorghum with higher phenolic contents generally had higher inhibitory activity. Among the tested extracts, the brown sorghum (IS131C)-bran-free extract (BR-bran-free, half-maximal inhibitory concentration (IC50) = 18 ± 11 mg sorghum/mL) showed the strongest inhibition against α-glucosidase which was comparable to that of acarbose (IC50 = 1.39 ± 0.23 mg acarbose/mL). The red sorghum (Mr-Buster)-kernel-bound extract (RM-kernel-bound, IC50 = 160 ± 12 mg sorghum/mL) was the most potent in inhibiting α-amylase but was much weaker compared to acarbose (IC50 = 0.50 ± 0.03 mg acarbose/mL).

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