scholarly journals OsbHLH073 Negatively Regulates Internode Elongation and Plant Height by Modulating GA Homeostasis in Rice

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 547
Author(s):  
Jinwon Lee ◽  
Sunok Moon ◽  
Seonghoe Jang ◽  
Sichul Lee ◽  
Gynheung An ◽  
...  
Keyword(s):  
Plant Height ◽  
Molecular Mechanisms ◽  
Agronomic Traits ◽  
Internode Elongation ◽  
Functional Identification ◽  
Dwarf Phenotype ◽  
Quantitative Expression ◽  
Helix Loop Helix ◽  
Transgenic Lines ◽  
Two Hybrid

Internode elongation is one of the key agronomic traits determining a plant’s height and biomass. However, our understanding of the molecular mechanisms controlling internode elongation is still limited in crop plant species. Here, we report the functional identification of an atypical basic helix-loop-helix transcription factor (OsbHLH073) through gain-of-function studies using overexpression (OsbHLH073-OX) and activation tagging (osbhlh073-D) lines of rice. The expression of OsbHLH073 was significantly increased in the osbhlh073-D line. The phenotype of osbhlh073-D showed semi-dwarfism due to deficient elongation of the first internode and poor panicle exsertion. Transgenic lines overexpressing OsbHLH073 confirmed the phenotype of the osbhlh073-D line. Exogenous gibberellic acid (GA3) treatment recovered the semi-dwarf phenotype of osbhlh073-D plants at the seedling stage. In addition, quantitative expression analysis of genes involving in GA biosynthetic and signaling pathway revealed that the transcripts of rice ent-kaurene oxidases 1 and 2 (OsKO1 and OsKO2) encoding the GA biosynthetic enzyme were significantly downregulated in osbhlh073-D and OsbHLH073-OX lines. Yeast two-hybrid and localization assays showed that the OsbHLH073 protein is a nuclear localized-transcriptional activator. We report that OsbHLH073 participates in regulating plant height, internode elongation, and panicle exsertion by regulating GA biosynthesis associated with the OsKO1 and OsKO2 genes.

scholarly journals Identification of Bna.IAA7.C05 as allelic gene for dwarf mutant generated from tissue culture in oilseed rape

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongtao Cheng ◽  
Fenwei Jin ◽  
Qamar U. Zaman ◽  
Bingli Ding ◽  
Mengyu Hao ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Height ◽  
High Throughput Sequencing ◽  
Agronomic Traits ◽  
Crop Improvement ◽  
Dwarf Mutant ◽  
Sequencing Analysis ◽  
Rna Seq ◽  
Dwarf Phenotype ◽  
Lodging Resistance

Abstract Background Plant height is one of the most important agronomic traits in many crops due to its influence on lodging resistance and yield performance. Although progress has been made in the use of dwarfing genes in crop improvement, identification of new dwarf germplasm is still of significant interest for breeding varieties with increased yield. Results Here we describe a dominant, dwarf mutant G7 of Brassica napus with down-curved leaves derived from tissue culture. To explore the genetic variation responsible for the dwarf phenotype, the mutant was crossed to a conventional line to develop a segregating F2 population. Bulks were formed from plants with either dwarf or conventional plant height and subjected to high throughput sequencing analysis via mutation mapping (MutMap). The dwarf mutation was mapped to a 0.6 Mb interval of B. napus chromosome C05. Candidate gene analysis revealed that one SNP causing an amino acid change in the domain II of Bna.IAA7.C05 may contribute to the dwarf phenotype. This is consistent with the phenotype of a gain-of-function indole-3-acetic acid (iaa) mutant in Bna.IAA7.C05 reported recently. GO and KEGG analysis of RNA-seq data revealed the down-regulation of auxin related genes, including many other IAA and small up regulated response (SAUR) genes, in the dwarf mutant. Conclusion Our studies characterize a new allele of Bna.IAA7.C05 responsible for the dwarf mutant generated from tissue culture. This may provide a valuable genetic resource for breeding for lodging resistance and compact plant stature in B. napus.

scholarly journals Rice Transcription Factor OsWRKY55 Is Involved in the Drought Response and Regulation of Plant Growth

2021 ◽  
Vol 22 (9) ◽  
pp. 4337
Author(s):  
Kai Huang ◽  
Tao Wu ◽  
Ziming Ma ◽  
Zhao Li ◽  
Haoyuan Chen ◽  
...  
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Plant Height ◽  
Molecular Mechanisms ◽  
Crop Improvement ◽  
Critical Role ◽  
Luciferase Reporter ◽  
Mitogen Activated Protein ◽  
Two Hybrid ◽  
Dual Luciferase Reporter Assay

WRKY transcription factors (TFs) have been reported to respond to biotic and abiotic stresses and regulate plant growth and development. However, the molecular mechanisms of WRKY TFs involved in drought stress and regulating plant height in rice remain largely unknown. In this study, we found that transgenic rice lines overexpressing OsWRKY55 (OsWRKY55-OE) exhibited reduced drought resistance. The OsWRKY55-OE lines showed faster water loss and greater accumulation of hydrogen peroxide (H2O2) and superoxide radical (O2−·) compared to wild-type (WT) plants under drought conditions. OsWRKY55 was expressed in various tissues and was induced by drought and abscisic acid (ABA) treatments. Through yeast two-hybrid assays, we found that OsWRKY55 interacted with four mitogen-activated protein kinases (MAPKs) that could be induced by drought, including OsMPK7, OsMPK9, OsMPK20-1, and OsMPK20-4. The activation effects of the four OsMPKs on OsWRKY55 transcriptional activity were demonstrated by a GAL4-dependent chimeric transactivation assay in rice protoplasts. Furthermore, OsWRKY55 was able to reduce plant height under normal conditions by decreasing the cell size. In addition, based on a dual luciferase reporter assay, OsWRKY55 was shown to bind to the promoter of OsAP2-39 through a yeast one-hybrid assay and positively regulate OsAP2-39 expression. These results suggest that OsWRKY55 plays a critical role in responses to drought stress and the regulation of plant height in rice, further providing valuable information for crop improvement.

scholarly journals Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mao Yang ◽  
Jianbo He ◽  
Shubei Wan ◽  
Weiyan Li ◽  
Wenjing Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Height ◽  
Oilseed Rape ◽  
Molecular Mechanisms ◽  
Dominant Gene ◽  
Planting Density ◽  
Protein Sequence Analysis ◽  
Dwarf Phenotype ◽  
Lodging Resistance ◽  
Dwarfing Gene

Abstract Background Plant height is an important architecture trait which is a fundamental yield-determining trait in crops. Variety with dwarf or semi-dwarf phenotype is a major objective in the breeding because dwarfing architecture can help to increase harvest index, increase planting density, enhance lodging resistance, and thus be suitable for mechanization harvest. Although some germplasm or genes associated with dwarfing plant type have been carried out. The molecular mechanisms underlying dwarfism in oilseed rape (Brassica napus L.) are poorly understood, restricting the progress of breeding dwarf varieties in this species. Here, we report a new dwarf mutant Bndwarf2 from our B. napus germplasm. We studied its inheritance and mapped the dwarf locus BnDWARF2. Results The inheritance analysis showed that the dwarfism phenotype was controlled by one semi-dominant gene, which was mapped in an interval of 787.88 kb on the C04 chromosome of B. napus by Illumina Brassica 60 K Bead Chip Array. To fine-map BnDWARF2, 318 simple sequence repeat (SSR) primers were designed to uniformly cover the mapping interval. Among them, 15 polymorphic primers that narrowed down the BnDWARF2 locus to 34.62 kb were detected using a F2:3 family population with 889 individuals. Protein sequence analysis showed that only BnaC04.BIL1 (BnaC04g41660D) had two amino acid residues substitutions (Thr187Ser and Gln399His) between ZS11 and Bndwarf2, which encoding a GLYCOGEN SYNTHASE KINASE 3 (GSK3-like). The quantitative real-time PCR (qRT-PCR) analysis showed that the BnaC04.BIL1 gene expressed in all tissues of oilseed rape. Subcellular localization experiment showed that BnaC04.BIL1 was localized in the nucleus in tobacco leaf cells. Genetic transformation experiments confirmed that the BnaC04.BIL1 is responsible for the plant dwarf phenotype in the Bndwarf2 mutants. Overexpression of BnaC04.BIL1 reduced plant height, but also resulted in compact plant architecture. Conclusions A dominant dwarfing gene, BnaC04.BIL1, encodes an GSK3-like that negatively regulates plant height, was mapped and isolated. Our identification of a distinct gene locus may help to improve lodging resistance in oilseed rape.

scholarly journals Effects of BrMYC2/3/4 on Plant Development, Glucosinolate Metabolism, and Sclerotinia sclerotiorum Resistance in Transgenic Arabidopsis thaliana

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhiyan Teng ◽  
Weiwei Zheng ◽  
Youjian Yu ◽  
Seung-Beom Hong ◽  
Zhujun Zhu ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Height ◽  
Sclerotinia Sclerotiorum ◽  
Plant Development ◽  
Shoot Elongation ◽  
35S Promoter ◽  
Bhlh Transcription Factor ◽  
Seed Length ◽  
Helix Loop Helix ◽  
Transgenic Lines

MYC2/3/4, known as a basic helix–loop–helix (bHLH) transcription factor, directly activate the genes involved in diverse plant development and secondary metabolites biosynthesis. In this study, we identified and cloned five MYC paralogs (BrMYC2/3-1/3-2/4-1/4-2) from Chinese cabbage (Brassica rapa ssp. pekinensis). In-silico analyses for the physicochemical properties suggested that BrMYC2/3-1/3-2/4-2/4-3 are unstable hydrophobic and acidic proteins, while BrMYC4-1 is an unstable hydrophobic and basic protein. BrMYC2/3/4 belong to the bHLH superfamily and are closely related to AthMYC2/3/4 orthologs that mediate the regulation of various secondary metabolites. It was demonstrated that BrMYC2/3/4-GFP fusion protein localized in the nucleus and expression levels of five BrMYC2/3/4 homologous genes all elevated relative to control (Ctrl). When expressed in Arabidopsis under the control of 35S promoter, each of the BrMYC2/3-1/3-2/4-1/4-2 transgenes differentially influenced root and shoot elongation, vegetative phase change, flowering time, plant height and tiller number after flowering, and seed production. Despite the variation of phenotypes between the transgenic lines, all the lines except for BrMYC4-2 exhibited shorter seed length, less seed weight, higher accumulation of glucosinolates (GSs), and resistance to Sclerotinia sclerotiorum than Ctrl. Notably, BrMYC2 overexpression (OE) line significantly reduced the lengths of root and hypocotyl, seed length, and weight, along with faster bolting time and strikingly higher accumulation of total GSs. Accumulation of GSs at the highest levels in the BrMYC2OE line conferred the highest resistance to S. sclerotiorum. Unlike BrMYC3OE and BrMYC4OE, BrMYC2OE stimulated the growth of plant height after fluorescence. The results of this study point to the BrMYC2 overexpression that may provide a beneficial effect on plant growth and development via plant resistance to the fungal pathogen.

scholarly journals A BIN2-like1 Protein Confers Dwarf by Interacting with BZR1 of Brassinosteroid Signaling in Allotetraploid Brassica Napus

2021 ◽  
Author(s):  
Mao Yang ◽  
Jianbo He ◽  
Shubei Wan ◽  
Weiyan Li ◽  
Wenjing Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Plant Height ◽  
Plant Architecture ◽  
Molecular Mechanisms ◽  
Glycogen Synthase ◽  
Molecular Evidence ◽  
Dwarf Phenotype ◽  
Brassinosteroid Signaling ◽  
Successive Generations

Abstract Brassinosteroids (BRs) are steroid hormones that play essential roles in plant growth and development. In this study, we identified a new dwarf mutant in Brassica napus. By map-based cloning, BnaC04.BIL1 (BnaC04g41660D) gene, a BIN2-like1 (BIL1) encoding a GLYCOGEN SYNTHASE KINASE 3 (GSK3-like) protein kinase, was isolated. To date, how BIL1 involves in BR signal transduction remains uncovered. Genetic transformation experiments confirmed that the BnaC04.BIL1 is responsible for the plant dwarf phenotype in the Bndwarf2 mutants. Overexpression of BnaC04.BIL1 not only reduced plant height, but also resulted in compact plant architecture. Using CRISPR/Cas9, two sgRNAs were designed to target BnaC04.BIL1 gene. The gene editing experiments generated mutations of BnaC04.BIL1 sequence, which were stably transmitted to successive generations, and lead to restoration of plant height and plant architecture. The molecular mechanism of Bndwarf2 dwarfing was further verified by Y2H and BiFC assays. Results shown that a Thr187Ser amino acid substitution residing in the conserved region promotes the interaction between BnaC04.BIL1Mut with BnaBZR1, thus enhances the negative regulation of plant growth. The genetic and molecular evidence clarifies first the BnaC04.BIL1 can sharply change plant architecture in natural plant accessions in allotetraploid, and provides new insights into the molecular mechanisms of BR signaling.

scholarly journals Genetic mapping and transcriptomic characterization of a new fuzzless-tufted cottonseed mutant

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qian-Hao Zhu ◽  
Warwick Stiller ◽  
Philippe Moncuquet ◽  
Stuart Gordon ◽  
Yuman Yuan ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Agronomic Traits ◽  
Gene Families ◽  
Mutant Phenotype ◽  
Wild Type ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Flanking Region ◽  
Comparative Transcriptomic Analysis

Abstract Fiber mutants are unique and valuable resources for understanding the genetic and molecular mechanisms controlling initiation and development of cotton fibers that are extremely elongated single epidermal cells protruding from the seed coat of cottonseeds. In this study, we reported a new fuzzless-tufted cotton mutant (Gossypium hirsutum) and showed that fuzzless-tufted near-isogenic lines (NILs) had similar agronomic traits and a higher ginning efficiency compared to their recurrent parents with normal fuzzy seeds. Genetic analysis revealed that the mutant phenotype is determined by a single incomplete dominant locus, designated N5. The mutation was fine mapped to an approximately 250-kb interval containing 33 annotated genes using a combination of bulked segregant sequencing, SNP chip genotyping, and fine mapping. Comparative transcriptomic analysis using 0–6 days post-anthesis (dpa) ovules from NILs segregating for the phenotypes of fuzzless-tufted (mutant) and normal fuzzy cottonseeds (wild-type) uncovered candidate genes responsible for the mutant phenotype. It also revealed that the flanking region of the N5 locus is enriched with differentially expressed genes (DEGs) between the mutant and wild-type. Several of those DEGs are members of the gene families with demonstrated roles in cell initiation and elongation, such as calcium-dependent protein kinase and expansin. The transcriptome landscape of the mutant was significantly reprogrammed in the 6 dpa ovules and, to a less extent, in the 0 dpa ovules, but not in the 2 and 4 dpa ovules. At both 0 and 6 dpa, the reprogrammed mutant transcriptome was mainly associated with cell wall modifications and transmembrane transportation, while transcription factor activity was significantly altered in the 6 dpa mutant ovules. These results imply a similar molecular basis for initiation of lint and fuzz fibers despite certain differences.

scholarly journals Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus

2021 ◽  
Vol 9 (2) ◽  
pp. 304
Author(s):  
Yao Chi ◽  
Li-Long Pan ◽  
Shu-Sheng Liu ◽  
Shahid Mansoor ◽  
Xiao-Wei Wang
Keyword(s):  
Coat Protein ◽  
Molecular Mechanisms ◽  
Cotton Leaf ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Vacuolar Protein ◽  
Leaf Curl Disease ◽  
Two Hybrid ◽  
Asia Ii 1 ◽  
Leaf Curl

Cotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the Bemisia tabaci complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms underlying the transmission are poorly known. In this study, we attempted to identify the whitefly proteins involved in CLCuMuV transmission. First, using a yeast two-hybrid system, we identified 54 candidate proteins of Asia II 1 that putatively can interact with the coat protein of CLCuMuV. Second, we examined interactions between the CLCuMuV coat protein and several whitefly proteins, including vacuolar protein sorting-associated protein (Vps) twenty associated 1 (Vta1). Third, using RNA interference, we found that Vta1 positively regulated CLCuMuV acquisition and transmission by the Asia II 1 whitefly. In addition, we showed that the interaction between the CLCuMuV coat protein and Vta1 from the whitefly Middle East-Asia Minor (MEAM1), a poor vector of CLCuMuV, was much weaker than that between Asia II 1 Vta1 and the CLCuMuV coat protein. Silencing of Vta1 in MEAM1 did not affect the quantity of CLCuMuV acquired by the whitefly. Taken together, our results suggest that Vta1 may play an important role in the transmission of CLCuMuV by the whitefly.

Differential use of SCL/TAL-1 DNA-binding domain in developmental hematopoiesis

Blood ◽  
2008 ◽  
Vol 112 (4) ◽  
pp. 1056-1067 ◽  
Author(s):  
Mira T. Kassouf ◽  
Hedia Chagraoui ◽  
Paresh Vyas ◽  
Catherine Porcher
Keyword(s):  
Dna Binding ◽  
Molecular Mechanisms ◽  
Binding Activity ◽  
Hematopoietic Stem ◽  
Helix Loop Helix ◽  
Experimental Systems ◽  
Dna Binding Activity ◽  
Independent Functions

Abstract Dissecting the molecular mechanisms used by developmental regulators is essential to understand tissue specification/differentiation. SCL/TAL-1 is a basic helix-loop-helix transcription factor absolutely critical for hematopoietic stem/progenitor cell specification and lineage maturation. Using in vitro and forced expression experimental systems, we previously suggested that SCL might have DNA-binding–independent functions. Here, to assess the requirements for SCL DNA-binding activity in vivo, we examined hematopoietic development in mice carrying a germline DNA-binding mutation. Remarkably, in contrast to complete absence of hematopoiesis and early lethality in scl-null embryos, specification of hematopoietic cells occurred in homozygous mutant embryos, indicating that direct DNA binding is dispensable for this process. Lethality was forestalled to later in development, although some mice survived to adulthood. Anemia was documented throughout development and in adulthood. Cellular and molecular studies showed requirements for SCL direct DNA binding in red cell maturation and indicated that scl expression is positively autoregulated in terminally differentiating erythroid cells. Thus, different mechanisms of SCL's action predominate depending on the developmental/cellular context: indirect DNA binding activities and/or sequestration of other nuclear regulators are sufficient in specification processes, whereas direct DNA binding functions with transcriptional autoregulation are critically required in terminal maturation processes.

scholarly journals Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zaiqing Wang ◽  
Anmin Yu ◽  
Fei Li ◽  
Wei Xu ◽  
Bing Han ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Castor Bean ◽  
Woody Plants ◽  
Molecular Mechanisms ◽  
Ricinus Communis ◽  
Bulked Segregant Analysis ◽  
Oilseed Crop ◽  
Yeast Transformation ◽  
Dwarf Phenotype ◽  
Genetic Breeding

AbstractPlant dwarfism is a desirable agronomic trait in non-timber trees, but little is known about the physiological and molecular mechanism underlying dwarfism in woody plants. Castor bean (Ricinus communis) is a typical woody oilseed crop. We performed cytological observations within xylem, phloem and cambia tissues, revealing that divergent cell growth in all tissues might play a role in the dwarf phenotype in cultivated castor bean. Based on bulked segregant analyses for a F2 population generated from the crossing of a tall and a dwarf accession, we identified two QTLs associated with plant height, covering 325 candidate genes. One of these, Rc5NG4-1 encoding a putative IAA transport protein localized in the tonoplast was functionally characterized. A non-synonymous SNP (altering the amino acid sequence from Y to C at position 218) differentiated the tall and dwarf plants and we confirmed, through heterologous yeast transformation, that the IAA uptake capacities of Rc5NG4-1Y and Rc5NG4-1C were significantly different. This study provides insights into the physiological and molecular mechanisms of dwarfing in woody non-timber economically important plants, with potential to aid in the genetic breeding of castor bean and other related crops.

scholarly journals Genetic control of agronomic traits in an oat population of recombinant lines

2010 ◽  
Vol 10 (4) ◽  
pp. 305-311 ◽  
Author(s):  
Itamar Cristiano Nava ◽  
Ismael Tiago de Lima Duarte ◽  
Marcelo Teixeira Pacheco ◽  
Luiz Carlos Federizzi
Keyword(s):  
Grain Yield ◽  
Genetic Control ◽  
Plant Height ◽  
Agronomic Traits ◽  
Test Weight ◽  
Phenotypic Traits ◽  
Phenotypic Data ◽  
Plant Growth Habit ◽  
Vegetative Cycle ◽  
Efficiency Of Selection

Understanding the genetic control of phenotypic traits is essential to increase the efficiency of selection for adapted, high-yielding genotypes. The purpose of this study was to determine the genetic control of nine traits of hexaploid oat. Phenotypic data were collected from a population of 162 recombinant lines derived from the cross 'UFRGS17 x UFRGS 930598-6'. For the traits plant growth habit, hairs on leaf edges and panicle type, monogenic genetic control was observed. A quantitative and/or polygenic genetic control was stated for the traits panicle weight, panicle length, vegetative cycle, plant height, test weight and grain yield. High heritability was estimated for the traits vegetative cycle (h² = 0.89) and plant height (h² = 0.79), while moderate heritability was determined for test weight (h² = 0.51) and grain yield (h² = 0.48).

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