scholarly journals Nutritional Content and Antioxidant Capacity of the Seed and the Epicarp in Different Ecotypes of Pistacia atlantica Desf. Subsp. atlantica

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1065 ◽  
Author(s):  
Amina Labdelli ◽  
Abdelkrim Rebiai ◽  
Mohammed Tahirine ◽  
Ahmed Adda ◽  
Othmane Merah
Keyword(s):  
Human Health ◽  
High Performance ◽  
Biological Activities ◽  
Reversed Phase ◽  
Essential Elements ◽  
Phenolic Composition ◽  
Nutritional Values ◽  
Pistacia Atlantica ◽  
Phenolic Contents ◽  
Calcium Phosphorus

Phenolic compounds are secondary metabolites that occur naturally in all plants. Seeds are among the richest organs of plants in phytochemicals, vitamins and minerals. These compounds and their biological activities are of great importance for human health. This study aimed to analyze the phenolic composition and their antioxidant activity in the seeds and epicarps of six Algerian populations of Pistacia atlantica Desf. subsp. atlantica growing along an aridity gradient from semi-arid to Saharan environmental conditions. Higher phenolic contents were observed in epicarp compared to seeds whatever the ecotype. The highest phenolic content of seeds and epicarps was observed in ecotype of Djelfa and the lowest values in Tiaret (T-Z). Phenolic composition, measured by reversed-phase high-performance liquid chromatography (HPLC), showed that quercetin in epicarp, gallic and chlorogenic acids in seeds were the most present in all ecotypes. Large differences were observed between ecotypes for nutritional values. Seeds were rich in flavonoids, proteins, carbohydrates and essential elements such as potassium, calcium, phosphorus and iron. These results highlighted the potential importance of Atlas pistachio fruits as a source of essential compounds that contribute to human health. Moreover, this underused species may serve a potential source for antioxidant components for alimentation and cosmetics purposes.

scholarly journals HPLC - DAD Analysis of Lycorine in Amaryllidaceae Species

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500
Author(s):  
Gulen Irem Kaya ◽  
Derya Cicek ◽  
Buket Sarıkaya ◽  
Mustafa Ali Onur ◽  
Nehir Unver Somer
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Array Detector ◽  
Simple Method ◽  
Aerial Parts ◽  
Liquid Chromatographic Method ◽  
Low Mass ◽  
Liquid Chromatographic

Lycorine, the most frequent alkaloid found in Amaryllidaceae plants, has been proven to have various biological activities. Therefore, it is important to quantify this compound in Amaryllidacaeae species. In this study, a reversed-phase high performance liquid chromatographic method has been used and validated for the determination of lycorine in Amaryllidaceae plants. A simple method for the extraction of lycorine in low-mass plant samples was employed utilizing columns pre-packed with diatomaceous earth (Extrelut®). This method was applied to the aerial parts and bulbs of Sternbergia sicula Tineo ex Guss., S. lutea (L.) Ker-Gawl. ex Sprengel and Pancratium maritimum L. (Amaryllidaceae) collected during two different vegetation periods. The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:90:10) applied at a flow rate 1 mL min−1 using diode array detector. Validation procedures showed that the method was specific, accurate and precise. The amount of lycorine in S. sicula samples ranged between 0.10-0.53%, whereas in those of S. lutea and P. maritimum, the range was 0.19-0.40% and 0.05-0.14%, respectively.

scholarly journals Taxonomical, Phytochemical, Traditional Explanation, Nutritional Values, and Biological Activities of Certain Edible Medicinal Plants of Tripura, India

2021 ◽  
Vol 21 (3) ◽  
pp. 173
Author(s):  
Bikash Debnath ◽  
Waikhom Somraj Singh ◽  
Sanchari Goswami ◽  
Kuntal Manna
Keyword(s):  
Medicinal Plants ◽  
Human Health ◽  
Chronic Diseases ◽  
Plant Species ◽  
Nutritional Value ◽  
Biological Activities ◽  
Edible Plants ◽  
Nutritional Values ◽  
Active Constituents ◽  
Nutritional Imbalance

Edible plants are used as valuable sources of food and medicine to prevent nutritional imbalance, illness, and maintenance of human health. This review covers botanical descriptions, phytochemicals studies, traditional comprehensions, dietary values, and biological activities of ten vegetable plants and eight fruit plants of Tripura, India. The book “The Flora of Tripura State” helps to choose the plant species of Tripura, India and PubMed, NCBI, Google scholar databases have been used to describe the plant species briefly. Literature reveals that all the selected edible plants contain bio-active constituents (alkaloids, phenols, flavonoids, tannins, terpenoids, glycosides, etc.) and give dynamic biological activities. All the plants have a high nutritional value. This review believes it will provide significant advances in the prevention of malnutrition and chronic diseases.

scholarly journals Differentiation of Phenolic Composition Among Tunisian Thymus algeriensis Boiss. et Reut. (Lamiaceae) Populations: Correlation to Bioactive Activities

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 515 ◽  
Author(s):  
Jaouadi ◽  
Silva ◽  
Boussaid ◽  
Yahia ◽  
Cardoso ◽  
...  
Keyword(s):  
Significant Variation ◽  
High Performance ◽  
Array Detector ◽  
Gram Negative Bacteria ◽  
Phenolic Composition ◽  
Phenolic Contents ◽  
Methanolic Extracts ◽  
Thymus Algeriensis ◽  
Escherchia Coli ◽  
Bioclimatic Zone

: Twelve Tunisian Thymus algeriensis populations growing wild in different bioclimatic zones, extending from the subhumid to the upper-arid bioclimates, were compared regarding their phenolic composition and their ability to serve as antioxidant, anti-acetylcholinesterase, and antibacterial agents. A significant variation of phenol profile was observed between the analyzed populations, as assessed by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MSn) technique. Rosmarinic acid was the main phenolic compound in most populations (383.8–1157.8 µg/mL extract), but still, those from the upper-arid bioclimatic zone were distinguished by the presence of carvacrol (1374.7 and 2221.6 µg/mL extract), which was absent in the remaining ones. T. algeriensis methanolic extracts were found to possess a substantial antioxidant and anti-acetylcholinesterase activities, with significant variation observed between populations, which were correlated to their phenolic contents. The antibacterial activity of the extracts tested against seven bacteria was revealed only by populations collected from upper-arid bioclimate and mainly associated with the presence of carvacrol. Extracts revealed a bacteriostatic effect against all bacteria (MIC = 1.4 mg/mL). Yet, the bactericidal activity (MBC = 1.4mg/mL) was restricted to the gram-negative bacteria Escherchia coli.

scholarly journals Two Tachykinin-Related Peptides with Antimicrobial Activity Isolated from Triatoma infestans Hemolymph

2020 ◽  
Vol 13 ◽  
pp. 117863612093363
Author(s):  
Laura Cristina Lima Diniz ◽  
Flávio Lopes Alves ◽  
Antonio Miranda ◽  
Pedro Ismael da Silva Junior
Keyword(s):  
Antimicrobial Activity ◽  
Amino Acid ◽  
Secondary Structure ◽  
High Performance ◽  
Micrococcus Luteus ◽  
Biological Activities ◽  
Biological Effects ◽  
Vero Cells ◽  
Reversed Phase ◽  
Triatoma Infestans

Antimicrobial peptides and proteins (AMPs) are molecules that can interact with microbial cells and lead to membrane disruption or intracellular molecule interactions and death. Several molecules with antimicrobial effects also present other biological activities. One such protein group representing the duplicity of activities is the tachykinin family. Tachykinins (TKs) form a family of neuropeptides in vertebrates with a consensus C-terminal region (F-X-G-Y-R-NH2). Invertebrate TKs and TK-related peptides (TKRPs) are subfamilies found in invertebrates that present high homology with TKs and have similar biological effects. Several of these molecules have already been described but reports of TKRP in Hemiptera species are limited. By analyzing the Triatoma infestans hemolymph by reversed-phase high-performance liquid chromatography, biological assays, and mass spectrometry, two antimicrobial molecules were isolated and identified as TKRPs, which we named as TRP1-TINF and TRP2-TINF (tachykinin-related peptides I and II from T. infestans). TRP1-TINF is a random secondary structure peptide with 9 amino acid residues. It is susceptible to aminopeptidases degradation and is active mainly against Micrococcus luteus (32 μM). TRP2-TINF is a 10-amino acid peptide with a 310 helix secondary structure and is susceptible to carboxypeptidases degradation. It has major antimicrobial activity against both Pseudomonas aeruginosa and Escherichia coli (45 μM). Neither molecule is toxic to human erythrocytes and both present minor toxicity toward Vero cells at a concentration of 1000 μM. As the first description of TKRPs with antimicrobial activity in T. infestans, this work contributes to the wider comprehension of the insects’ physiology and describes pharmacological relevant molecules.

scholarly journals Effect of Ripening on the Phenolic Composition and Mineral Content of Three Varieties of Olive Fruits

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 380
Author(s):  
María del Pilar Fernández-Poyatos ◽  
Eulogio J. Llorent-Martínez ◽  
Antonio Ruiz-Medina
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
High Performance ◽  
Mineral Content ◽  
Weather Conditions ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Mineral Contents ◽  
Inductively Coupled ◽  
Phenolic Contents

The phenolic composition and mineral content of Cornezuelo, Cornicabra and Picual olive fruit varieties were investigated during olive ripening in two different harvesting seasons (2017/2018 and 2018/2019). Phytochemical profiles were evaluated by high-performance liquid chromatography (HPLC) with diode-array and mass spectrometry detection. Mineral contents were determined by inductively coupled plasma-mass spectrometry (ICP-MS). Twenty-five compounds were characterized and the main ones quantified. These compounds corresponded mostly to secoiridoids, the main ones being oleuropein, oleoside/secologanoside, oleoside-11-methylester, and oleuropein and comselogoside isomers. Total phenolic contents reached the highest values between December and January, coinciding with the usual harvesting date. This trend was observed in both harvesting seasons, although higher phenolic contents were recorded in season 2018/2019. This was due to the different weather conditions, which caused a lower olive production in season 2017/2018. No clear tendency was observed between mineral content and harvest time in any of the studied seasons. The highest concentration of total phenolics was obtained in Cornezuelo variety (840 mg/100 g) in January 2019 (season 2018/2019). Picual and Cornicabra varieties reached concentrations of 670 mg/100 g and 530 mg/100 g, respectively, also in the last harvesting dates of season 2018/2019.

scholarly journals Influence of Extraction Solvent on the Phenolic Profile and Bioactivity of Two Achillea Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1601
Author(s):  
Dominika Kaczorová ◽  
Erna Karalija ◽  
Sabina Dahija ◽  
Renata Bešta-Gajević ◽  
Adisa Parić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Phenolic Composition ◽  
Extraction Solvent ◽  
Ethanolic Extracts ◽  
Liquid Chromatography Tandem Mass

The phenolic composition, as well as the antioxidant and antimicrobial activities of two poorly investigated Achillea species, Achillea lingulata Waldst. and the endemic Achillea abrotanoides Vis., were studied. To obtain a more detailed phytochemical profile, four solvents with different polarities were used for the preparation of the plant extracts whose phenolic composition was analyzed using UHPLC-MS/MS (ultra-high performance liquid chromatography-tandem mass spectrometry). The results indicate that both of the investigated Achillea species are very rich in both phenolic acids and flavonoids, but that their profiles differ significantly. Chloroform extracts from both species had the highest yields and were the most chemically versatile. The majority of the examined extracts showed antimicrobial activity, while ethanolic extracts from both species were potent against all tested microorganisms. Furthermore, the antioxidant activity of the extracts was evaluated. It was found that the ethanolic extracts possessed the strongest antioxidant activities, although these extracts did not contain the highest amounts of detected phenolic compounds. In addition, several representatives of phenolic compounds were also assayed for these biological activities. Results suggest that ethanol is a sufficient solvent for the isolation of biologically active compounds from both Achillea species. Moreover, it was shown that the flavonoids naringenin and morin are mainly responsible for these antimicrobial activities, while caffeic, salicylic, chlorogenic, p-coumaric, p-hydroxybenzoic, and rosmarinic acid are responsible for the antioxidant activities of the Achillea extracts.

scholarly journals Employing an R Software Package rsm for Optimizing of Genistein, Daidzein, and Glycitein Separation and Its Application for Soy Milk Analysis by HPLC Method

2020 ◽  
Vol 20 (5) ◽  
pp. 1184
Author(s):  
Florentinus Dika Octa Riswanto ◽  
Alni Desra ◽  
Rinjani Mustika Sari ◽  
Valentino Thomas ◽  
Abdul Rohman ◽  
...  
Keyword(s):  
High Performance ◽  
Quantitative Estimation ◽  
Biological Activities ◽  
Reversed Phase ◽  
Hplc Method ◽  
Milk Analysis ◽  
Soy Milk ◽  
Hplc Separation ◽  
Column Temperature ◽  
System Suitability

Soy milk, one of the soybean products, become more popular in recent years due to its benefit for human health. Biological activities of soybean products have been widely studied according to the presence of isoflavone aglycones content, including genistein, daidzein, and glycitein. Hence, it is important to develop an effective and efficient analytical method to provide guidance regarding appropriate isoflavone intake levels for soy milk. A reversed-phase high performance liquid chromatography (HPLC) method was developed and optimized in this study employed by R statistical software with the package of rsm. A C18 column was used for HPLC separation with the detection at 260 nm. Optimized condition for HPLC separation has been achieved with the mobile phase of methanol: water (63.26:36.74), a flow rate of 0.81 mL/min, and a column temperature of 45.31 °C. These conditions were applied in the HPLC system and successfully tested for the system suitability. Quantitative estimation was performed and resulted that the genistein, daidzein, and glycitein content in soy milk samples were 6.372, 6.273, and 2.853 µg/mL, respectively.

Development and Validation of A Reversed Phase-High Performance Liquid Chromatography Method for the Quantitative Estimation of Ramipril Drug Content from Formulated Dosage Form

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Uv Detector ◽  
Chromatography Method ◽  
Drug Content

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.

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