Response of Horticultural Soil Microbiota to Different Fertilization Practices

Environmentally friendly agricultural production necessitates manipulation of microbe–plant interactions, requiring a better understanding of how farming practices influence soil microbiota. We studied the effect of conventional and organic treatment on soil bacterial richness, composition, and predicted functional potential. 16S rRNA sequencing was applied to soils from adjacent plots receiving either a synthetic or organic fertilizer, where two crops were grown within treatment, homogenizing for differences in soil properties, crop, and climate. Conventional fertilizer was associated with a decrease in soil pH, an accumulation of Ag, Mn, As, Fe, Co, Cd, and Ni; and an enrichment of ammonia oxidizers and xenobiotic compound degraders (e.g., Candidatus Nitrososphaera, Nitrospira, Bacillus, Pseudomonas). Soils receiving organic fertilization were enriched in Ti (crop biostimulant), N, and C cycling bacteria (denitrifiers, e.g., Azoarcus, Anaerolinea; methylotrophs, e.g., Methylocaldum, Methanosarcina), and disease-suppression (e.g., Myxococcales). Some predicted functions, such as glutathione metabolism, were slightly, but significantly enriched after a one-time manure application, suggesting the enhancement of sulfur regulation, nitrogen-fixing, and defense of environmental stressors. The study highlights that even a single application of organic fertilization is enough to originate a rapid shift in soil prokaryotes, responding to the differential substrate availability by promoting soil health, similar to recurrent applications.

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Protists as main indicators and determinants of plant performance

Microbiome ◽

10.1186/s40168-021-01025-w ◽

2021 ◽

Vol 9 (1) ◽

Author(s):

Sai Guo ◽

Wu Xiong ◽

Xinnan Hang ◽

Zhilei Gao ◽

Zixuan Jiao ◽

...

Keyword(s):

Plant Growth ◽

Organic Fertilizer ◽

Agricultural Practices ◽

Plant Performance ◽

Organic Fertilization ◽

Plant Yield ◽

Growing Seasons ◽

Beneficial Microorganisms ◽

Plant Growth Promoting ◽

Plant Growth Promoting Microbes

Abstract Background Microbiomes play vital roles in plant health and performance, and the development of plant beneficial microbiomes can be steered by organic fertilizer inputs. Especially well-studied are fertilizer-induced changes on bacteria and fungi and how changes in these groups alter plant performance. However, impacts on protist communities, including their trophic interactions within the microbiome and consequences on plant performance remain largely unknown. Here, we tracked the entire microbiome, including bacteria, fungi, and protists, over six growing seasons of cucumber under different fertilization regimes (conventional, organic, and Trichoderma bio-organic fertilization) and linked microbial data to plant yield to identify plant growth-promoting microbes. Results Yields were higher in the (bio-)organic fertilization treatments. Soil abiotic conditions were altered by the fertilization regime, with the prominent effects coming from the (bio-)organic fertilization treatments. Those treatments also led to the pronounced shifts in protistan communities, especially microbivorous cercozoan protists. We found positive correlations of these protists with plant yield and the density of potentially plant-beneficial microorganisms. We further explored the mechanistic ramifications of these relationships via greenhouse experiments, showing that cercozoan protists can positively impact plant growth, potentially via interactions with plant-beneficial microorganisms including Trichoderma, the biological agent delivered by the bio-fertilizer. Conclusions We show that protists may play central roles in stimulating plant performance through microbiome interactions. Future agricultural practices might aim to specifically enhance plant beneficial protists or apply those protists as novel, sustainable biofertilizers.

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In situ nitrogen mineralization and nutrient release by soil amended with black soldier fly frass fertilizer

Scientific Reports ◽

10.1038/s41598-021-94269-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Dennis Beesigamukama ◽

Benson Mochoge ◽

Nicholas Korir ◽

Changeh J. Ghemoh ◽

Sevgan Subramanian ◽

...

Keyword(s):

Soil Acidity ◽

Organic Fertilizer ◽

Soil Health ◽

Nutrient Release ◽

Tropical Soils ◽

Control Treatment ◽

Black Soldier Fly ◽

Amended Soils ◽

Cropping Seasons ◽

Bacteria And Fungi

AbstractAlthough black soldier fly frass fertilizer (BSFFF) is effective on crop performance, information on nitrogen (N) mineralization and nutrient release capacity of soils amended with BSFFF is lacking. This study utilized field incubation experiments to investigate the ammonification, nitrification, microbial populations, and quantities of nutrients released by soils amended with BSFFF and commercial organic fertilizer (SAFI) for a period equivalent to two maize cropping seasons. For the control treatment, no BSFFF or SAFI was added. Results indicated that most of the N in BSFFF amended soils was available in the ammonium form, while soils treated with SAFI had higher nitrate concentration. The BSFFF amended soils experienced shorter net immobilization periods of N (30–60 days) compared to SAFI treated soils (60–95 days). Increased rates of mineralization (3–10 times) and nitrification (2–4 times) were observed in soils treated with BSFFF during the second season of application. The BSFFF treated soils showed significantly higher N, phosphorus, and magnesium release than the control. Repeated application of BSFFF led to increased N release by three-folds in the soil. Furthermore, soil amendment with BSFFF increased the populations of bacteria and fungi, reduced soil acidity, and increased phosphorus (two-folds) and magnesium (two–four-folds) release than SAFI treated soils. Our findings highlight the crucial role of BSFFF in improving soil health by addressing the challenges of soil acidity, phosphorus fixation and nutrient mining, which is characteristic of most tropical soils.

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Bromatology of Tifton 85 Grass Irrigated with Two Qualities of Water and under Organic Fertilization

Journal of Agricultural Science ◽

10.5539/jas.v9n11p172 ◽

2017 ◽

Vol 9 (11) ◽

pp. 172

Author(s):

Maria Teresa Cristina Coelho do Nascimen ◽

Joelma Sales dos Santos ◽

Carlos Alberto Vieira de Azevedo ◽

Vera Lucia Antunes de Lima ◽

Rubens Barrichello Gomes Barbosa

Keyword(s):

Crude Protein ◽

Nutritional Value ◽

Organic Fertilizer ◽

Domestic Wastewater ◽

Neutral Detergent Fiber ◽

Organic Fertilization ◽

Randomized Experimental Design ◽

Nutritional Needs ◽

Set Up ◽

Higher Doses

The reuse of solid and liquid wastes has become a viable practice for the sustainability of agricultural production, because it meets the nutritional needs of crops and also allows the use of nutrients present in these residues. The objective of this work was to analyze the nutritional value of Tifton 85 irrigated with treated domestic wastewater and fertilized with nitrogen doses from poultry bed in four successive cuts, with frequency of 35 days. The experiment was set up in a protected environment, arranged in a completely randomized experimental design, in a 5 × 2 factorial scheme, with four replications. Five doses of nitrogen were tested through organic fertilization from avian beds (0, 15, 30, 45 and 60 kg N ha-1) and two irrigation water qualities (treated domestic well and artesian well). After the cuts the material was dried and sent to the laboratory to determine the following parameters: crude protein (CP), neutral detergent fiber (NDF) and Tifton 85 acid detergent fiber (ADF). The highest values of crude protein were obtained using the domestic wastewater treated in the irrigation, as well as using the higher doses of nitrogen from poultry litter. Just as the NDF and ADF contents presented the best values when treated domestic wastewater and the highest nitrogen doses. Indicating that irrigation with wastewater and the use of organic fertilizer may substitute for conventional manures by promoting satisfactory levels of concentrations for fodder.

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Impact of Long-Term Organic Fertilizer Application on Lignin in Mollisol

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.113-116.1332 ◽

2010 ◽

Vol 113-116 ◽

pp. 1332-1335 ◽

Cited By ~ 1

Author(s):

Ning Liu ◽

Hong Bo He ◽

Hong Tu Xie ◽

Zhen Bai ◽

Xu Dong Zhang

Keyword(s):

Organic Carbon ◽

Organic Fertilizer ◽

Fertilizer Application ◽

Carbon Pools ◽

Organic Fertilization ◽

Degradation Degree ◽

Northeast Of China ◽

The Impact ◽

Cuo Oxidation

Fertilization is one of the essential managements to maintain and increase soil organic carbon (SOC) level in agroecosystems. It has been realized that fertilizer applications influenced the turnover of labile and refractory organic carbon pools in arable soil markedly. However, the dynamic of relatively refractory lignin in response to fertilization is still kept unclear. Therefore, the impact of long-term organic fertilization on the content and degradation degree of lignin in Mollisol was investigated. Lignin monomers were released by alkaline CuO oxidation method and quantified by gas chromatography (GC). At the time scale of decades, lignin was clearly accumulated in soil and the relative accumulation of lignin in SOC was evident after long-term organic fertilizer application. Compared with the unfertilized soil, lower acid to aldehyde ratios of vanillyl and syringyl units induced by organic fertilization suggested a lower degradation degree of lignin incorporated into soil to some extent. It could be concluded that long-term organic fertilization was an effective fertilizer practice for lignin accumulation in soil and SOC sequestration in Mollisol in northeast of China.

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Jatropha press cake as organic fertilizer in lettuce cultivation

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v20n12p1089-1094 ◽

2016 ◽

Vol 20 (12) ◽

pp. 1089-1094

Author(s):

José R. Mantovani ◽

Jéssica da S. Bernardes ◽

Paulo R. C. Landgraf

Keyword(s):

Soil Acidity ◽

Press Cake ◽

Block Design ◽

Organic Fertilizer ◽

Phosphate Fertilizer ◽

Soil Samples ◽

Biodiesel Production ◽

Organic Fertilization ◽

Lettuce Seedling ◽

Randomized Block Design

ABSTRACT In biodiesel production, vegetable press cake is obtained as waste, and a suitable destination for jatropha press cake would be the use in agriculture as organic fertilizer. This study aimed to evaluate the effect of jatropha press cake on soil fertility attributes and on lettuce production and accumulation of nutrients in the shoots. The experiment was conducted in pots in a greenhouse, in a randomized block design with eight treatments and five replicates. The treatments consisted of doses of jatropha press cake equivalent to 0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0 and 32.0 t ha-1. Portions of 5.5 dm3 of soil received limestone, phosphate fertilizer and the doses of jatropha press cake, and remained incubated for 30 days. After incubation, soil samples were collected, each pot received one lettuce seedling and the experiment was carried out for 45 days. The organic fertilization with jatropha press cake increased the contents of nutrients in the soil, especially K+, but caused increment in soil acidity and electrical conductivity. The use of jatropha press cake as organic fertilizer decreased lettuce production and accumulation of nutrients in the shoots.

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Identification and characterization of proteins, lipids, and metabolites in two organic fertilizer products derived from different nutrient sources

Applied Biological Chemistry ◽

10.1186/s13765-021-00625-2 ◽

2021 ◽

Vol 64 (1) ◽

Author(s):

Jianyu Li ◽

Xin Zhao ◽

Laura S. Bailey ◽

Manasi N. Kamat ◽

Kari B. Basso

Keyword(s):

Biochemical Composition ◽

Organic Fertilizer ◽

Saturated Fatty Acids ◽

Soil Health ◽

Soil Microbial Communities ◽

Organic Fertilizers ◽

Vegetable Production ◽

Limited Information ◽

Nutrient Sources ◽

Soil Microbial

AbstractThe biochemical composition of organic fertilizers largely determines their nutrient supply characteristics following soil application as well as their potential impact on soil microbial communities. Yet, limited information is available regarding the biochemical composition of organic fertilizers derived from different nutrient sources. Here, we qualitatively analyzed the presence and abundance of proteins, lipids, and metabolites in a liquid fish fertilizer (LFF) product and a type of granular organic fertilizer (GOF) commonly used in organic vegetable production, using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Our results suggest that the presence and abundance of proteins, lipids, and metabolites differ greatly between GOF and LFF. The qualitative analysis shows LFF as a rich source of metabolites, while complex proteins and long-chain saturated fatty acids are dominant in GOF. The degree of biochemical composition complexity may help explain the varying impacts of different types of organic fertilizers on nutrient availability, soil health, and environmental quality.

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Response of vegetative growth and chemical properties in tomato plant (Tariz) for organic fertilization.

10.36602/jmuas.2019.v01.01.05 ◽

2019 ◽

pp. 51-66

Author(s):

Halemah M. Amhamed ◽

Ahmed. I. Sharaf ◽

Salaheddin B. Elbelazi ◽

Moftah A. Assofal ◽

Amal E. Alhishni

Keyword(s):

Tomato Plant ◽

Agricultural Land ◽

Block Design ◽

Organic Fertilizer ◽

Chemical Properties ◽

Cattle Manure ◽

Control Treatment ◽

Average Weight ◽

Organic Fertilization ◽

And Chemical Properties

The experiment was carried out in agricultural land in Sorman between 4 - 2017 to 7 - 2017, in the design of Randomized Complete Block Design (RCBD) to study the response of vegetative traits and chemical properties in the tomato plant of Tariz for organic fertilization, by 6 treatments and 3 replicates, For SPSS statistical analysis at a significant level of LSD 0.05). The experiment included four types of semi-decomposed organic fertilizer (sheep manure, cattle manure, camel manure, horse manure) in 6 treatments, in addition to the control treatment, which included four treatments for each type of organic fertilizer as: fertilizer: soil by ratio (1: 3) that is 5Kg of fertilizer : 15 kg of soil, mixed treatment of fertilizer : soil (1: 1) (10 kg of fertilizer: 10 kg of soil), and double treatment (3: 1) (15 kg of fertilizer: 5 kg soil). The seedlings were planted in experimental units of 7.20 m2. The seedlings were treated in biological control using the garlic extract for insect protection after 15 days of planting, as well as the addition of simple proportions of hay as a cover to reduce the growth of herbs. The experiment showed that the treatment of cattle manure significantly increased most of the studied morphological traits (length of root, plant length, number of flowers, number of leafy branches), as well as significant increase in fresh and dry weight of leaves and average weight of five fruits.. There were significant differences in the content of the N, P in leaves and NPK elements in the fruits, while there was a significant increase in vitamin C content in fruits for all treatments.

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Atributos químicos de Latossolo sob plantio direto adubado com esterco de bovinos e fertilizantes minerais

Comunicata Scientiae ◽

10.14295/cs.v6i3.527 ◽

2015 ◽

Vol 6 (3) ◽

pp. 263 ◽

Cited By ~ 4

Author(s):

Milena Barcellos ◽

Antonio Carlos Vargas Motta ◽

Volnei Pauletti ◽

José Carlos Peixoto Modesto Da Silva ◽

Julierme Zimmer Barbosa

Keyword(s):

Organic Fertilizer ◽

Mineral Fertilizer ◽

Mineral Fertilizers ◽

Organic Fertilization ◽

Organic C ◽

Tillage System ◽

High Production ◽

Chemical Attributes ◽

Four Levels ◽

Total Organic C

Organic fertilization in no-tillage system (NTS) has been used in regions milk production, in an order to provide nutrients for crops and provide a destination for high production of animal waste. The aim of this study was to evaluate the chemical attributes of an Oxisol, a function of organic fertilization with liquid manure from dairy cattle (LMDC) and mineral fertilizers. The experiment was conducted under NTS with crop rotation including legumes and grasses. The treatments were distributed in three randomized blocks with factorial arrangement, using three levels of mineral fertilizer (0, 50 and 100 % of the dose recommended for the crops) and four levels of organic fertilizer (0, 30, 60 and 90 m3 ha-1year-1). After six years of fertilizer management of crops, soil was collected from five depths (0-0.05, 0.05-0.10, 0.10-0.30, 0.30-0.50 e 0.50-0.80 m). The samples were determined the chemical pH, H++Al3+, Al3+, Ca2+, Mg2+, K+,electrical conductivity (EC), bases saturation (V), Ca2+/Mg2+ ratio, total organic C (TOC), B, Cl, Fe, Cu, Zn and Mn. The mineral fertilizers acidify the soil, raised the K+, P and EC and, changed the Zn and Cu contents. The organic fertilization with LMDC was a source of nutrients (Ca 2+, Mg2+, K+, P, Zn and Cu), raised the TOC and the EC, and kept the soil acidity attributes (pH, H++ Al3+ and V).

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Organik Matter: It’s Role in Sustainable Farming of Sugarcane

Perspektif ◽

10.21082/p.v14n1.2015.61-71 ◽

2016 ◽

Vol 14 (1) ◽

pp. 61

Author(s):

Djajadi Djajadi

Keyword(s):

Organic Matter ◽

Soil Organic Matter ◽

Soil Fertility ◽

Green Manure ◽

Organic Fertilizer ◽

Soil Health ◽

High Yield ◽

Cane Yield ◽

Sustainable Farming

ABSTRACTOrganik matter has an important role in determining soil health of sugarcane, i.e. soil capacity to support sugarcane to produce sustainable high yield. Soil organic matter influences soil physical, chemical, and biological properties, so that a consequence of declining soil organic matter is poorer soil fertility and lower yield. This paper has an objective to elucidate the important role of organic matter on sustainable farming of sugarcane. The important role of organic matter in soil fertility has been known for a long time before Green Revolution concept was introduced. With more intensity in sugarcane farming and more increasing of sugar demand, application of organic fertilizer started to be substituted by chemical fertilizer. Using green manure and/or biofertilizer has a chance to be spread out to the farmers due to more practical and more efficient than solid organik fertilizer, such as dung manure or compost. Future research should be focusing on the efectivity of green manure and or biofertilzer sources in improving soil fertility and cane yield, minimizing soil pathogen, reducing soil erosion of sugar cane land monoculture, and improving awareness of farmers about soil degradation as consequences of sugarcane monoculture planting for years.Keywords: Organic matter, sugarcane, soil health sustainable farming Bahan Organik: Peranannya dalam Budidaya Tebu BerkelanjutanABSTRAKBahan organik tanah berperan penting dalam menentukan kesehatan tanah tebu, yaitu kapasitas tanah yang dapat mendukung produksi tebu yang tinggi secara berkelanjutan. Kadar bahan organik tanah mempengaruhi sifat fisik, kimia dan biologi tanah. Paper ini bertujuan untuk menguraikan tentang peranan bahan organik dalam memperbaiki sifat fisik, kimia dan biologi tanah pertanaman tebu. Pentingnya peran bahan oganik tersebut sudah disadari dari dulu, sehingga sebelum revolusi hijau penggunaan pupuk organik sudah umum dilakukan petani. Dengan semakin intensifnya budidaya tebu dan semakin meningkatnya kebutuhan gula, pemanfaatan pupuk organik sudah jarang dilakukan. Diperlukan usaha untuk meningkatkan dan mempertahankan kadar bahan organik pada lahan tebu, antara lain berupa gerakan masal dalam bentuk gerakan nasional melalui program aplikasi bahan organik. Pemanfaatan pupuk hijau dan/atau pupuk hayati berpeluang untuk diterapkan karena lebih praktis dan efisien daripada penambahan pupuk organik padat. Penelitian ke depan perlu difokuskan untuk mengkaji jenis-jenis pupuk organik dan pupuk hayati yang efektif memperbaiki kesuburan, dalam menekan serangan penyakit, meminimalkan erosi pada lahan-lahan tebu monokultur, dan meningkatkan kesadaran petani tebu tentang terjadinya degradasi lahan akibat penanaman tebu yang terus menerus.Kata kunci: Bahan organik, tebu, kesehatan tanah, budidaya berkelanjutan

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Sweet Onion (Allium cepa L.) as Influenced by Organic Fertilization Rate: 1. Plant Growth, and Leaf and Bulb Mineral Composition

HortScience ◽

10.21273/hortsci12791-17 ◽

2018 ◽

Vol 53 (4) ◽

pp. 451-458

Author(s):

Juan Carlos Díaz-Pérez ◽

Jesús Bautista ◽

Gunawati Gunawan ◽

Anthony Bateman ◽

Cliff Martin Riner

Keyword(s):

Plant Growth ◽

Allium Cepa ◽

Organic Fertilizer ◽

The United States ◽

Fertilization Rate ◽

Mineral Nutrients ◽

N Leaching ◽

Nutrient Deficiencies ◽

Organic Fertilization ◽

Onion Plant

Vidalia onions (Allium cepa L.) are sweet, short-day, low pungency, yellow Granex-type bulbs that are popular in the United States because of their mild flavor. There are limited studies on sweet onion plant growth in response to organic fertilization rate. The objective of this report was to evaluate the effects of organic fertilizer rates on sweet onion plant growth, and leaf and bulb mineral nutrients. Experiments were carried out at the Horticulture Farm, Tifton Campus, University of Georgia, in the Winters of 2012–13 and 2013–14. There were five treatments [organic fertilizer 3–2–3 equivalent to 0, 60, 120, 180, and 240 kg·ha−1 nitrogen (N)]. During the season and at the mature plant stage, root, stem, and bulb biomass increased whereas the root-to-shoot ratio decreased with increasing fertilization rate up to 120 kg·ha−1 N. Foliar concentrations of N and Ca decreased whereas Cu concentration increased with increasing organic fertilization rate. Bulb Mg and Mn increased whereas P and Cu decreased with increasing organic fertilization rate. The accumulation of mineral nutrients by onion whole plants increased quadratically (N, P, K, and S) or linearly (Ca and Mg) with increasing fertilization rate. The N use efficiency decreased with increasing organic fertilization rate; the agronomic efficiency of N (AEN) decreased quadratically and the marginal yield decreased linearly with increasing fertilization rate. Chlorophyll indices (CI) were highest with 240 kg·ha−1 N and lowest with 0 kg·ha−1 N. In conclusion, onion plant growth increased with increasing organic fertilizer rate probably because of augmented soil N levels. Observation of nutrient deficiencies late in the season, even at high organic fertilization rates, indicates that preplant application of organic fertilizer was sufficient to cover plant nutritional needs only partially and that applications of N fertilizer later in the season may be necessary. High application rates of organic fertilizer (above those required by the crop) may have resulted in significant N leaching because it is unlikely that the crop used most of the N that was mineralized. Bulb concentrations of P, K, Ca, Mg, S, B, Fe, Cu, and Mn were higher compared with values reported in the literature for onions produced with inorganic fertilizers.

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