Electrospun Polyvinylpyrrolidone-Gelatin and Cellulose Acetate Bi-Layer Scaffold Loaded with Gentamicin as Possible Wound Dressing

Acceleration of wound healing can be achieved with the use of wound dressings. Through the electrospinning technique, a polymeric scaffold composed of two layers was processed: a gelatin and polyvinylpyrrolidone layer with gentamicin, and a second layer of cellulose acetate. The conditions for the electrospinning process were standardized for voltage parameters, feed flow and the distance from the injector to the collector. Once the values of the main variables for the electrospinning were optimized, a three-hour processing time was established to allow the separation of the material from the collector. The obtained material was characterized by observations on scanning electron microscopy, Fourier transform infrared spectroscopy and thermal analysis; contact angle measurement was performed to evaluate wettability properties, and antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus were evaluated using the Kirby–Bauer test. The obtained fibers that form the bi-layer scaffold present diameters from 100 to 300 nm. The scaffold presents chemical composition, thermal stability, wettability characteristics and antibacterial activity that fulfill the proposal from this study, based on obtaining a scaffold that could be used as a drug delivery vehicle and a wound dressing material.

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Preparation of Cross-linked Chitosan Quaternary Ammonium Salt Hydrogel Films Loading Drug of Gentamicin Sulfate for Antibacterial Wound Dressing

Marine Drugs ◽

10.3390/md19090479 ◽

2021 ◽

Vol 19 (9) ◽

pp. 479

Author(s):

Jingjing Zhang ◽

Wenqiang Tan ◽

Qing Li ◽

Xiaorui Liu ◽

Zhanyong Guo

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ammonium Salt ◽

Quaternary Ammonium ◽

Quaternary Ammonium Salt ◽

Wound Dressing ◽

Cross Linking ◽

And Staphylococcus Aureus ◽

Hydrogel Films

Hydrogels, possessing high biocompatibility and adaptability to biological tissue, show great usability in medical applications. In this research, a series of novel cross-linked chitosan quaternary ammonium salt loading with gentamicin sulfate (CTMCSG) hydrogel films with different cross-linking degrees were successfully obtained by the reaction of chitosan quaternary ammonium salt (TMCS) and epichlorohydrin. Fourier transform infrared spectroscopy (FTIR), thermal analysis, and scanning electron microscope (SEM) were used to characterize the chemical structure and surface morphology of CTMCSG hydrogel films. The physicochemical property, gentamicin sulphate release behavior, cytotoxicity, and antibacterial activity of the CTMCSG against Escherichia coli and Staphylococcus aureus were determined. Experimental results demonstrated that CTMCSG hydrogel films exhibited good water stability, thermal stability, drug release capacity, as well as antibacterial property. The inhibition zone of CTMCSG hydrogel films against Escherichia coli and Staphylococcus aureus could be up to about 30 mm. Specifically, the increases in maximum decomposition temperature, mechanical property, water content, swelling degree, and a reduction in water vapor permeability of the hydrogel films were observed as the amount of the cross-linking agent increased. The results indicated that the CTMCSG-4 hydrogel film with an interesting physicochemical property, admirable antibacterial activity, and slight cytotoxicity showed the potential value as excellent antibacterial wound dressing.

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Investigation of Superhydrophobic/Hydrophobic Materials Properties Using Electrospinning Technique

Baghdad Science Journal ◽

10.21123/bsj.2019.16.3.0632 ◽

2019 ◽

Vol 16 (3) ◽

pp. 0632 ◽

Cited By ~ 2

Author(s):

Jaafar Et al.

Keyword(s):

Polymer Solution ◽

Glass Substrate ◽

Low Cost ◽

Contact Angle Measurement ◽

Angle Measurement ◽

Metal Substrate ◽

Electrospinning Technique ◽

Electrospinning Method ◽

Surface Alteration ◽

Measurement Surface

The aim of this research is to study the surface alteration characteristics and surface morphology of the superhydrophobic/hydrophobic nanocomposite coatings prepared by an electrospinning method to coat various materials such as glass and metal. This is considered as a low cost method of fabrication for polymer solutions of Polystyrene (PS), Polymethylmethacrylate (PMMA) and Silicone Rubber (RTV). Si were prepared in various wt% of composition for each solutions. Contact angle measurement, surface tension, viscosity, roughness tests were calculated for all specimens. SEM showed the morphology of the surfaces after coated. PS and PMMA showed superhydrophobic properties for metal substrate, while Si showed hydrophobic characteristics for both metal and glass substrate. Polymer solution of (15%Si/Thinner (Th)) owned best roughness for glass substrate and polymer solution of (4%PMMA/Tetrahydrofuran (THF)) owned best roughness for metal substrate.

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Effect of Solution Miscibility on the Morphology of Coaxial Electrospun Cellulose Acetate Nanofibers

Polymers ◽

10.3390/polym13244419 ◽

2021 ◽

Vol 13 (24) ◽

pp. 4419

Author(s):

Ke Yan ◽

Yao Le ◽

Hu Mengen ◽

Li Zhongbo ◽

Huang Zhulin

Keyword(s):

Cellulose Acetate ◽

Shell Structure ◽

Partial Solution ◽

Electrospinning Process ◽

Core Shell ◽

Electrospinning Technique ◽

Product Morphology ◽

The Core ◽

Core Shell Structure ◽

Structural Characterizations

Coaxial electrospinning (co-electrospinning) technique has greatly expanded the universality of fabricating core-shell polymer nanofibers. However, the effect of solution miscibility on the morphology of co-electrospun products remains unclear. Herein, different cellulose acetate (CA) solutions with high solution miscibility but distinctly different electrospinnability were used to survey the effect of solution miscibility on the co-electrospinning process. The structural characterizations show that co-electrospun products are composed of nanofibers with and without the core-shell structure. This indicates that partial solution mixing occurred during the co-electrospinning process instead of absolute no-mixing or complete mixing. Importantly, the solution miscibility also shows a significant influence on the product morphology. In particular, the transformation from nanofibers to microparticles was realized with the increase of core-to-shell flow ratio during the co-electrospinning of core electrosprayable CA/dimethylacetamide (DMAc) solution and shell electrospinnable CA/acetone-DMAc (2/1, v/v) solution. Results show that the solution miscibility exerts a significant effect on not only the formation of core-shell structure but also the product morphology. This work provides a new insight for the in-depth understanding of the co-electrospinning process.

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Composite Wound Dressing Based on Chitin/Chitosan Nanofibers: Processing and Biomedical Applications

Cosmetics ◽

10.3390/cosmetics6010016 ◽

2019 ◽

Vol 6 (1) ◽

pp. 16 ◽

Cited By ~ 11

Author(s):

Anton Shabunin ◽

Vladimir Yudin ◽

Irina Dobrovolskaya ◽

Evgeny Zinovyev ◽

Viktor Zubov ◽

...

Keyword(s):

Wound Dressing ◽

High Efficiency ◽

Healing Process ◽

Experimental Studies ◽

Scar Tissue ◽

Wound Dressings ◽

Control Group ◽

Wound Healing Process ◽

Electrospinning Technique

An electrospinning technique was used for the preparation of a bilayered wound dressing consisting of a layer of aliphatic copolyamide nanofibers and a layer of composite nanofibers from chitosan and chitin nanofibrils filler. Processed dressings were compared with aliphatic copolyamide nanofiber-based wound dressings and control groups. Experimental studies (in vivo treatment of third-degree burns with this dressing) demonstrated that almost complete (up to 97.8%) epithelialization of the wound surface had been achieved within 28 days. Planimetric assessment demonstrated a significant acceleration of the wound healing process. Histological analysis of scar tissue indicated the presence of a significant number of microvessels and a low number of infiltrate cells. In the target group, there were no deaths or purulent complications, whereas in the control group these occurred in 25% and 59.7% of cases, respectively—and, in the copolyamide group, 0% and 11%, respectively. The obtained data show the high efficiency of application of the developed composite chitosan‒copolyamide wound dressings for the treatment of burn wounds.

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Synthesis and Characterization of Polycaprolactone/Cellulose Acetate by Electrospinning for Wound Dressing Applications

Materials Science Forum ◽

10.4028/www.scientific.net/msf.981.291 ◽

2020 ◽

Vol 981 ◽

pp. 291-295

Author(s):

Nurul Nadirah Suteris ◽

Izan Izwan Misnon ◽

Rasidi Roslan ◽

Farah Hanani Zulkifli ◽

Jayarama Reddy Venugopal ◽

...

Keyword(s):

Cellulose Acetate ◽

Wound Dressing ◽

Functional Materials ◽

Recent Time ◽

Synthesis And Characterization ◽

Environmental Load ◽

High Quality ◽

Electrospinning Technique ◽

Renewable Material

Cellulose as a renewable material has received enormous interest in recent time with an effort to minimize the environmental load from mining earthborn functional materials as well as reducing carbon footprint. This work demonstrates that high quality cellulose could be produced from empty fruit bunch of oil palm plantation and could be developed into nanofibers. A small amount of poly (ε-caprolactone) (PCL) was added to the EFB driven cellulose acetate (CA) to develop them as nanofibers by electrospinning technique; this composition was further enhanced by adding curcumin, which is a natural anti-inflammatory, and compared their morphology, structure, mechanical and surface properties.

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Electrospun Gelatin Fibers Surface Loaded ZnO Particles as a Potential Biodegradable Antibacterial Wound Dressing

Nanomaterials ◽

10.3390/nano9040525 ◽

2019 ◽

Vol 9 (4) ◽

pp. 525 ◽

Cited By ~ 9

Author(s):

Yu Chen ◽

Weipeng Lu ◽

Yanchuan Guo ◽

Yi Zhu ◽

Yeping Song

Keyword(s):

Antibacterial Activity ◽

Wound Dressing ◽

Wound Dressings ◽

X Ray ◽

E Coli ◽

Particle Size Analyzer ◽

Zno Particles ◽

Electrospun Gelatin ◽

Dressing Materials ◽

Application Prospects

Traditional wound dressings require frequent replacement, are prone to bacterial growth and cause a lot of environmental pollution. Therefore, biodegradable and antibacterial dressings are eagerly desired. In this paper, gelatin/ZnO fibers were first prepared by side-by-side electrospinning for potential wound dressing materials. The morphology, composition, cytotoxicity and antibacterial activity were characterized by using Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), particle size analyzer (DLS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), thermogravimetry (TGA) and Incucyte™ Zoom system. The results show that ZnO particles are uniformly dispersed on the surface of gelatin fibers and have no cytotoxicity. In addition, the gelatin/ZnO fibers exhibit excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) with a significant reduction of bacteria to more than 90%. Therefore, such a biodegradable, nontoxic and antibacterial fiber has excellent application prospects in wound dressing.

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Preparation of CMC-PVA/PAN-FePc(COOH)8/CS-PVA Bipolar Membrane and its Application in Electro-Oxidized Synthesis of Dialdehydle Starch

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1120-1121.141 ◽

2015 ◽

Vol 1120-1121 ◽

pp. 141-147

Author(s):

Zhong Gui Li ◽

Ting Jin Zhou ◽

Ri Yao Chen ◽

Xiao Chen ◽

Xi Zheng ◽

...

Keyword(s):

Polyvinyl Alcohol ◽

Current Efficiency ◽

Current Density ◽

Cell Voltage ◽

Contact Angle Measurement ◽

Angle Measurement ◽

Experimental Results ◽

Bipolar Membrane ◽

Electrospinning Technique ◽

A Current

The polyacrylonitrile (PAN)-iron octocarboxyphthalocyanine (FePc(COOH)8) nanofibers were prepared using electrospinning technique and introduced into the interlayer of a carboxymethyl cellulose (CMC)-polyvinyl alcohol (PVA)/chitosan (CS)-polyvinyl alcohol bipolar membrane (BPM), which was characterized using SEM, contact angle measurement, current-voltage characteristics, AC impedance spectroscopy and so on. The experimental results showed that after modification by PAN-FePc(COOH)8 nanofibers, the membrane impedance of the BPM and its cell voltage were decreased. That indicated that the water splitting efficiency in the interlayer of the BPM was increased. Then the prepared CMC-PVA/PAN-FePc(COOH)8/CS-PVA BPM was used in the electro-oxidized preparation of dialdehydle starch (DAS). The experimental results indicated that a current density of 20mA·cm-2 was suitable to obtain high current efficiency. When the electrolysis time was 3h at a current density of 20 mA·cm-2 , the current efficiency of the CMC-PVA/PAN-FePc(COOH)8/CS-PVA BPM-equipped cell was as high as 67%.

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Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant

Journal of The Royal Society Interface ◽

10.1098/rsif.2014.0169 ◽

2014 ◽

Vol 11 (95) ◽

pp. 20140169 ◽

Cited By ~ 71

Author(s):

Shu He ◽

Ping Zhou ◽

Linxin Wang ◽

Xiaoling Xiong ◽

Yifei Zhang ◽

...

Keyword(s):

Antibacterial Activity ◽

Photoelectron Spectroscopy ◽

Biofilm Formation ◽

Contact Angle Measurement ◽

Angle Measurement ◽

One Step ◽

Bone Graft Substitutes ◽

Good Biocompatibility ◽

Adhesive Proteins

Implant-associated infections, which are normally induced by microbial adhesion and subsequent biofilm formation, are a major cause of morbidity and mortality. Therefore, practical approaches to prevent implant-associated infections are in great demand. Inspired by adhesive proteins in mussels, here we have developed a novel antibiotic-decorated titanium (Ti) material with enhanced antibacterial activity. In this study, Ti substrate was coated by one-step pH-induced polymerization of dopamine followed by immobilization of the antibiotic cefotaxime sodium (CS) onto the polydopamine-coated Ti through catechol chemistry. Contact angle measurement and X-ray photoelectron spectroscopy confirmed the presence of CS grafted on the Ti surface. Our results demonstrated that the antibiotic-grafted Ti substrate showed good biocompatibility and well-behaved haemocompatibility. In addition, the antibiotic-grafted Ti could effectively prevent adhesion and proliferation of Escherichia coli (Gram-negative) and Streptococcus mutans (Gram-positive). Moreover, the inhibition of biofilm formation on the antibiotic-decorated Ti indicated that the grafted CS could maintain its long-term antibacterial activity. This modified Ti substrate with enhanced antibacterial activity holds great potential as implant material for applications in dental and bone graft substitutes.

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Bi-Layered Disulfiram-Loaded Fiber Membranes With Antibacterial Properties for Wound Dressing

10.21203/rs.3.rs-535473/v1 ◽

2021 ◽

Author(s):

Chenchen Xie ◽

Jin Yan ◽

Siyuan Cao ◽

Ri Liu ◽

Baishun Sun ◽

...

Keyword(s):

Antibacterial Activity ◽

Wound Dressing ◽

Polyvinylidene Fluoride ◽

Antibacterial Properties ◽

Wound Dressings ◽

Plasma Modification ◽

Bottom Surface ◽

Chemical Compositions ◽

Fiber Membranes ◽

Loaded Fiber

Abstract In this study, the bi-layered disulfiram-loaded fiber membranes with antibacterial activity and different surface wettabilities are prepared using electrospinning technology. In the application of wound dressing, the hydrophilic surface of fiber membranes is beneficial for the cell adhesion and drug release to heal the wound, meanwhile the hydrophobic outside surface is able to block water penetration to reduce the probability of wound infection. The obtained bi-layered drug-loaded fiber membranes are composed of polyvinylidene fluoride (PVDF) bottom surface and disulfiram (DSF)/polylactic acid (PLA) top surface. To modify the top surface wettability, the oxygen plasma modification of bi-layered membranes was carried out. We analyzed the morphology, wettability and chemical compositions of bi-layered drug-loaded fiber membranes by various techniques. And the bi-layered disulfiram-loaded membranes showed potent antibacterial activity in vitro against both Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). Thus, the obtained bi-layered disulfiram-loaded fiber membranes are very suitable for wound dressings application.

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Fabrication and Evaluation of Polycaprolactone/Gelatin-Based Electrospun Nanofibers with Antibacterial Properties

Journal of Nanomaterials ◽

10.1155/2015/970542 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 25

Author(s):

Lor Huai Chong ◽

Mim Mim Lim ◽

Naznin Sultana

Keyword(s):

Drug Loading ◽

Antibacterial Properties ◽

Electrospun Nanofibers ◽

Contact Angle Measurement ◽

Angle Measurement ◽

Electrospinning Technique ◽

Water Contact ◽

Nanofibrous Scaffolds ◽

Model Drug

Nanofibrous scaffolds were fabricated through blending of a synthetic polymer, polycaprolactone (PCL), and a natural polymer, gelatin (GE), using an electrospinning technique. Processing and solution parameters were optimized to determine the suitable properties of PCL/GE-based nanofibers. Several characterizations were conducted to determine surface morphology by scanning electron microscopy (SEM), wettability using water contact angle measurement, and chemical bonding analysis using attenuated total reflectance (ATR) of PCL/GE-based nanofibers. Experimental results showed that 14% (w/v) PCL/GE with a flow rate of 0.5 mL/h and 18 kV demonstrated suitable properties. This nanofiber was then further investigated for itsin vitrodegradation, drug loading (using a model drug, tetracycline hydrochloride), and antibacterial testing (using zone inhibition method).

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