Zearalenone Removal from Corn Oil by an Enzymatic Strategy

The estrogen-like mycotoxin zearalenone (ZEN) is one of the most widely distributed contaminants especially in maize and its commodities, such as corn oil. ZEN degrading enzymes possess the potential for counteracting the negative effect of ZEN and its associated high safety risk in corn oil. Herein, we targeted enhancing the secretion of ZEN degrading enzyme by Pichia pastoris through constructing an expression plasmid containing three optimized expression cassettes of zlhy-6 codon and signal peptides. Further, we explored various parameters of enzymatic detoxification in neutralized oil and analyzed tocopherols and sterols losses in the corn oil. In addition, the distribution of degraded products was demonstrated as well by Agilent 6510 Quadrupole Time-of-Flight mass spectrometry. P. pastoris GSZ with the glucoamylase signal was observed with the highest ZLHY-6 secretion yield of 0.39 mg/mL. During the refining of corn oil, ZEN in the crude oil was reduced from 1257.3 to 13 µg/kg (3.69% residual) after neutralization and enzymatic detoxification. Compared with the neutralized oil, no significant difference in the total tocopherols and sterols contents was detected after enzymatic detoxification. Finally, the degraded products were found to be entirely eliminated by washing. This study presents an enzymatic strategy for efficient and safe ZEN removal with relatively low nutrient loss, which provides an important basis for further application of enzymatic ZEN elimination in the industrial process of corn oil production.

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Pengaruh Luka Iris Pada Tikus Dengan Paparan Hidrogen Peroxida 3% terhadap Epitelisasi dan Pembentukan Eksudat pada Permukaan Epitel Kulit.

Hang Tuah Medical journal ◽

10.30649/htmj.v17i2.438 ◽

2020 ◽

Vol 17 (2) ◽

pp. 172

Author(s):

HARMAN AGUSAPUTRA ◽

MARIA SUGENG ◽

AYLY SOEKAMTO ◽

ATIK WULANDARI

Keyword(s):

Hydrogen Peroxide ◽

Wound Healing ◽

The Other ◽

Delayed Wound Healing ◽

Peroxide Group ◽

Hemostatic Effect ◽

Significant Difference ◽

Negative Effect ◽

Wound Healing Effect ◽

Epithelial Growth

AbstractBackground: Hydrogen peroxide (H2O2) as antiseptic has been used frequently to clean woundsin in hospitals and clinics. Hydrogen peroxide has the effectof strong oxidative that can kill pathogens. It can clean up debris and necrotic tissuesin wounds. Hydrogen peroxidealso has hemostatic effect that can help to stop bleeding. Besides antiseptic effects, hydrogen peroxide i s suspected of having negative effect in wound healing. Hydrogen peroxide presumably could cause delayed wound healing by exudate formation and delayed epithelial growth.Method: This study was conducted in the laboratory using 48 white mice that were divided into 2 groups. All the mice were purposely wounded. Afterwards in one group the wounds were clean up using hydrogen peroxide, while in the other group without hydrogen peroxide as control. The wounds of both groups were observed on day 1, day 3 and day 7. On day 1 and day 3, both groups did not show significant difference.Result : on day 7 showed that the wound healing in hydrogen peroxide group were delayed. Fifty percent of them had the formation of exudate and 62.5% of them showed delayed epithelial growth.Conclusion : This study could show hydrogen peroxide as wound antiseptic has delayed wound healing effect.Keyword: hydrogen peroxide, wound healing

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Effect of Ridge Height, Row Grade, and Field Slope on Nutrient Losses in Runoff in Contour Ridge Systems under Seepage with Rainfall Condition

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18042022 ◽

2021 ◽

Vol 18 (4) ◽

pp. 2022

Author(s):

Juan An ◽

Jibiao Geng ◽

Huiling Yang ◽

Hongli Song ◽

Bin Wang

Keyword(s):

Polynomial Regression ◽

Influential Factors ◽

Nutrient Loss ◽

Rainfall Simulation ◽

N Loss ◽

Rainfall Condition ◽

Erosion Processes ◽

P Loss ◽

Ridge Height ◽

Negative Effect

Seepage plays a key role in nutrient loss and easily occurs in widely-used contour ridge systems due to the ponding process. However, the characteristics of nutrient loss and its influential factors under seepage with rainfall condition in contour ridge systems are still unclear. In this study, 23 seepage and rainfall simulation experiments are arranged in an orthogonal rotatable central composite design to investigate the role of ridge height, row grade, and field slope on Nitrate (NO3−–N) and Orthophosphate (PO4+3–P) losses resulting from seepage in contour ridge systems. In total, three types of NO3−–N and PO4+3–P loss were observed according to erosion processes of inter-rill–headward, inter-rill–headward–contour failure, and inter-rill–headward–contour failure–rill. Our results demonstrated that second-order polynomial regression models were obtained to predict NO3−–N and PO4+3–P loss with the independent variables of ridge height, row grade, and field slope. Ridge height was the most important factor for nutrient loss, with a significantly positive effect and the greatest contribution (52.35–53.47%). The secondary factor of row grade exerted a significant and negative effect, and was with a contribution of 19.86–24.11% to nutrient loss. The interaction between ridge height and row grade revealed a significantly negative effect on NO3−–N loss, whereas interactions among the three factors did not significantly affect PO4+3–P loss. Field slope only significantly affected NO3−–N loss. The optimal design of a contour ridge system to control nutrient loss was obtained at ridge height of 8 cm, row grade of 2°, and field slope of 6.5°. This study provides a method to assess and model nutrient loss, and improves guidance to implement contour ridge systems in terms of nutrient loss control.

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Baerveldt shunt surgery versus combined Baerveldt shunt and phacoemulsification: a prospective comparative study

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2017-310698 ◽

2017 ◽

Vol 102 (9) ◽

pp. 1248-1253 ◽

Cited By ~ 1

Author(s):

Mohamad El Wardani ◽

Ciara Bergin ◽

Kenza Bradly ◽

Eamon Sharkawi

Keyword(s):

Complication Rates ◽

Failure Rates ◽

Shunt Surgery ◽

Patient Demographics ◽

Aqueous Shunt ◽

Included Patient ◽

Significant Difference ◽

Negative Effect ◽

Prospective Comparative Study ◽

Baerveldt Tube

AimTo examine the efficacy and safety of Baerveldt tube (BT) implantation compared with combined phacoemulsification and Baerveldt tube (PBT) implantation in patients with refractory glaucoma.MethodsSeventy-six eyes of 76 patients were enrolled, 38 pseudophakic eyes underwent BT implantation alone and 38 phakic eyes underwent the BT implantation combined with phacoemulsification. Groups were matched for preoperative intraocular pressure (IOP) and number of glaucoma medications. Preoperative and postoperative measures recorded included patient demographics, visual acuity (VA), IOP, number of antiglaucoma medications and all complications. Patients were followed up for a minimum of 36 months. Failure was defined as: inadequate IOP control (IOP≤5 mm Hg/>21 mm Hg/<20% reduction from baseline, reoperation for glaucoma, loss of light perception vision, or removal of the implant).ResultsThere was a significant difference in failure rates between groups at 36 months (PBT 37% vs BT 15%, P=0.02). There was no significant difference for PBT versus BT in preoperative baseline ocular characteristics. At 36 months: median IOP=14 mm Hg vs 12 mm Hg, P=0.04; mean number of antiglaucomatous medications=1.7 vs 1.3, P=0.61; median VA=0.8 vs 0.7, P=0.44. Postoperative complication rates were similar in both groups (n=5 vs 5; 13% vs 13%).ConclusionsFailure rates were significantly greater in the PBT group at 3 years. Median IOP was also significantly higher in the PBT group. These results suggest that combining phacoemulsification with aqueous shunt surgery may have a negative effect on long-term shunt bleb survival.

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Impact of the Content Characteristic of Short Message Service Advertising on Consumer Attitudes

Social Behavior and Personality An International Journal ◽

10.2224/sbp.2014.42.9.1409 ◽

2014 ◽

Vol 42 (9) ◽

pp. 1409-1419 ◽

Cited By ~ 9

Author(s):

Hongyan Lin ◽

Xing Zhou ◽

Zhankui Chen

Keyword(s):

Structural Equation ◽

Short Message Service ◽

Consumer Attitudes ◽

Equation Modeling ◽

Short Message ◽

Mediating Role ◽

Significant Difference ◽

Message Service ◽

Attitudes Toward Advertising ◽

Negative Effect

We examined the influence of the overall content characteristic of short message service (SMS) advertising on consumer attitudes toward advertising on mobile devices. Attitudes of 246 Chinese (145 women, 101 men) toward permission-based SMS advertising received on electronic products were measured using structural equation modeling. Results showed that of the 4 dimensions of the overall content characteristic, entertainment, informativeness, and credibility positively and significantly influenced the attitude of consumers, whereas irritation had a negative effect. In addition, the overall content characteristic significantly impacted on consumer attitudes, with advertising value playing a mediating role. Finally, the influencing path coefficients revealed a significant difference according to gender. The credibility dimension had a greater impact on attitude toward the overall content characteristic for women than for men. Theoretical and practical implications are discussed.

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ANALISIS PERBEDAAN RASIO KEUANGAN BANK UMUM KONVESIONAL DAN BANK UMUM SYARIAH SERTA PENGARUHNYA TERHADAP PENYALURAN KREDIT ATAU PEMBIAYAAN (Studi empiris yang tedrdaftar di BEI periode 2013-2016)

Magisma: Jurnal Ilmiah Ekonomi dan Bisnis ◽

10.35829/magisma.v5i1.9 ◽

2017 ◽

Vol 5 (1) ◽

pp. 9-23

Author(s):

Dika Ismawati ◽

Edy Supriyono

Keyword(s):

Commercial Bank ◽

Commercial Banks ◽

Secondary Data ◽

Capital Adequacy ◽

Financial Ratios ◽

Operating Efficiency ◽

Analysis Technique ◽

Significant Difference ◽

Negative Effect ◽

General Data

This study aims to obtain evidence of differences in financial ratios of conventional commercial banks and sharia commercial banks, as well as the influence of financial ratios, liquidity, capital adequacy, problem loans, profitability, operating efficiency of lending. This study uses secondary data. The sample in this research is conventional commercial bank and sharia public bank listed on BEI with 4 year observation period (2013-2016). This sample was chosen by purposive sampling method. Independent sample t-test is used to test whether there is difference of average of two interconnected samples, multiple linear regression is used as data analysis technique as many as 148 general data of banking company, conventional bank and syariah commercial bank as many as 44 company data period 2013-2016 . The results of this study indicate that there are differences in average liquidity, problem loans, profitability, operating efficiency, While the average capital adequacy there is no significant difference between conventional commercial banks and sharia banks. While liquidity and profitability have a positive effect on lending, non performing loans and operating efficiency have a negative effect on lending, and capital adequacy does not affect credit disbursement.

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The Effect of Abiotic Stresses on the Protein Composition of Four Hungarian Wheat Varieties

Plants ◽

10.3390/plants11010001 ◽

2021 ◽

Vol 11 (1) ◽

pp. 1

Author(s):

Dalma Nagy-Réder ◽

Zsófia Birinyi ◽

Marianna Rakszegi ◽

Ferenc Békés ◽

Gyöngyvér Gell

Keyword(s):

Abiotic Stress ◽

Protein Content ◽

Functional Properties ◽

Crop Production ◽

Global Climate ◽

Protein Composition ◽

Grain Protein Content ◽

Wheat Varieties ◽

Significant Difference ◽

Negative Effect

Global climate change in recent years has resulted in extreme heat and drought events that significantly influence crop production and endanger food security. Such abiotic stress during the growing season has a negative effect on yield as well as on the functional properties of wheat grain protein content and composition. This reduces the value of grain, as these factors significantly reduce end-use quality. In this study, four Hungarian bread wheat cultivars (Triticum aestivum ssp. aestivum) with different drought and heat tolerance were examined. Changes in the size- and hydrophobicity-based distribution of the total proteins of the samples have been monitored by SE- and RP-HPLC, respectively, together with parallel investigations of changes in the amounts of the R5 and G12 antibodies related to celiac disease immunoreactive peptides. Significant difference in yield, protein content and composition have been observed in each cultivar, altering the amounts of CD-related gliadin, as well as the protein parameters directly related to techno-functional properties (Glu/Gli ratio, UPP%). The extent of changes largely depended on the timing of the abiotic stress. The severity of the negative effect depended on the growth stage in which abiotic stress occurred.

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Aberrant Lighting Causes Anxiety-like Behavior in Mice but Curcumin Ameliorates the Symptoms

Animals ◽

10.3390/ani11092590 ◽

2021 ◽

Vol 11 (9) ◽

pp. 2590

Author(s):

Dhondup Namgyal ◽

Kumari Chandan ◽

Sher Ali ◽

Ajaz Ahmad ◽

Maha J. Hashim ◽

...

Keyword(s):

Body Weight ◽

Research Field ◽

The Body ◽

Laboratory Animals ◽

Artificial Lighting ◽

Lighting Conditions ◽

Daily Food ◽

Significant Difference ◽

Negative Effect ◽

Lighting Systems

In the modern research field, laboratory animals are constantly kept under artificial lighting conditions. However, recent studies have shown the effect of artificial light on animal behavior and metabolism. In the present study on mice, following three weeks of housing in dim light at night (dLAN; 5lux) and complete darkness (DD; 0lux), we monitored the effect on body weight, daily food intake, anxiety-like behavior by employing the open field test, and expression of the period (PER1) gene. We also studied the effect of oral administration of different concentrations of curcumin (50, 100, and 150 mg/kg) for three weeks in the same mice and monitored these parameters. The exposure to dLAN had significantly increased the anxiety-like behavior and body weight possibly through the altered metabolism in mice, whereas exposure to DD caused increased anxiety but no significant difference in weight gain. Moreover, the expression of the PER1 gene involved in sleep was also found to be decreased in the aberrant light conditions (dLAN and DD). Although the treatment of curcumin had no effect on body weight, it ameliorated the anxiety-like behavior possibly by modulating the expression of the PER1 gene. Thus, alteration in the light/dark cycle had a negative effect on laboratory animals on the body weight and emotions of animals. The present study identifies the risk factors associated with artificial lighting systems on the behavior of laboratory animals and the ameliorative effects of curcumin, with a focus on anxiety-like behavior.

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Prevalence of somatic symptoms amongst undergraduate medical students of Marathwada region of Maharashtra, India

International Journal of Community Medicine and Public Health ◽

10.18203/2394-6040.ijcmph20205192 ◽

2020 ◽

Vol 7 (12) ◽

pp. 5106

Author(s):

Shaikh Arshiya Kaiser Husain ◽

Anwaya R. Magare ◽

Purushottam A. Giri ◽

Vijaykumar S. Jadhav

Keyword(s):

Medical Education ◽

Medical Students ◽

Medical Curriculum ◽

High Stress ◽

Well Being ◽

Undergraduate Medical Students ◽

Future Health ◽

Cross Sectional ◽

Significant Difference ◽

Negative Effect

Background: The aim of medical education is to produce competent, physically and mentally strong health professionals, as they are going to be the pillars of the future health care system. Stress is one of the most common and process-oriented obstacles in medical education. It often exerts a negative effect on the academic performance, physical health, and psychological well-being of the students. Dealing with overloaded medical curriculum, competing with peers, being away from home and meeting high expectations imposed by parents and society to excel is among the common stressful transitions at this stage.Methods: A cross-sectional descriptive study was carried out amongst 352 undergraduate medical students of a private medical college in a rural area of Maharashtra during April to October 2019. The structured questionnaire was used to record the data. Collected data was used to assess the severity of mental health issues among medical students.Results: Majority 194 (55.11%) students were in the age of 18 to 20 years followed by 141 (40.06%) were in 21 to 23 years. There were 196 (55.68%) girl students and 156 (44.32%) boys. According to the various categories, 80 (22.73%) of the students had low stress scores, followed by 76 (21.59%) in minimal. A highly significant difference in stress scores was seen between boys and girls, which was more in boys.Conclusions: Study concluded that undergraduate medical students perceive minimal to very high stress presented as various systems that vary with the year of study and gender wise too. There is a further need to look into the various causes of stress.

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The Effect of Thymol on Renal Toxicity Induced by Mercury Chloride in Rats

International Journal of Medical Laboratory ◽

10.18502/ijml.v8i3.7335 ◽

2021 ◽

Author(s):

Hamid Reza Jamshidi ◽

Faezeh Taheri

Keyword(s):

Mercuric Chloride ◽

Renal Toxicity ◽

Corn Oil ◽

Kidney Tissue ◽

Control Group ◽

Mercury Chloride ◽

Protective Effects ◽

Tissue Samples ◽

Significant Difference ◽

Anti Oxidant

Background and Aims: Mercuric chloride is highly toxic once absorbed into the bloodstream, especially the kidneys in which it is collected. Mercury chloride increases hydrogen peroxide and enhances the destruction of protective enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPX), leading to oxidative stress. Besides, thymol has anti-oxidant effects and can increase the activity of SOD and GPX. This study aims to evaluate the efficacy of thymol on mercury chloride-induced toxicity. Materials and Methods: In this study, 30 rats, consisting of 6 groups of 5, were used. Control group receiving a single dose of 0.5 mg/kg mercuric chloride for 15 days, third, fourth, and fifth group received intraperitoneal injection of mercuric chloride at a dose of 0.5 mg/kg for 15 days plus thymol at a dose of 10, 30, 50 mg/kg. The sixth group received mercuric chloride at a dose of 0.5 mg/kg for 15 days plus thymol at 30 mg/kg per day for ten days. Results: Results showed a significant difference in the activity of catalase enzyme in kidney tissue samples test. According to the results of SOD, there is a significant difference between the group of corn oil and the group of mercury chloride and between the group of mercury chloride and the group that receives thymol at a dose of 10, 30, 50 mg/kg (p ≤ 0.05). Conclusions: It can be concluded that mercury chloride-induced kidney toxicity and thymol have anti-oxidant protective effects for SOD and GPX.

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Deep Planting Has No Short- or Long-Term Effect on the Survival and Growth of White Spruce, Black Spruce, and Jack Pine

Northern Journal of Applied Forestry ◽

10.1093/njaf/28.3.146 ◽

2011 ◽

Vol 28 (3) ◽

pp. 146-151 ◽

Cited By ~ 10

Author(s):

Alain Paquette ◽

Jean-Pierre Girard ◽

Denis Walsh

Keyword(s):

Black Spruce ◽

White Spruce ◽

Jack Pine ◽

Eastern Canada ◽

Planting Depth ◽

Early Results ◽

Significant Difference ◽

Negative Effect ◽

Tukey Honestly Significant Difference

Abstract Although studies in the past have reported that the deeper planting of conifers has no effect on seedling performance, most planting guidelines in use today still recommend that seedlings be planted to the rootcollar. Past studies were mostly observational, used bareroot seedlings, and often reported early results from just one or two depths of planting treatments. Most of the results available regarding planting depth for boreal species are anecdotal, although they are planted by the hundreds of millions every year. The present study reports no short-term (1 year) or long-term (15 to 19 years) negative effect of planting depth on the survival and height and diameter growth of black spruce, white spruce, and jack pine seedlings over three large, replicated experiments in the boreal forest of eastern and northern Quebec (eastern Canada). Four different depth treatments were compared, from manual planting at the rootcollar to the deepest mechanical planting treatment at 10 cm or more, making this the largest, longest-lasting study of its kind. Although, as expected, important differences in growth were present between species, all three commonly planted conifers reacted similarly to the planting depth treatments (no effect). This result can in part be attributed to an almost perfect control of frost heaving in the deepest two treatments. Planting depth effects were assessed using analysis of variance, multiple Tukey honestly significant difference, and uncorrected pairwise one-tailed t-tests to increase the probability of detecting a negative effect. Absolute differences and effect sizes (generally small and often positive with greater depths) were also analyzed.

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