scholarly journals Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium

2016 ◽  
Vol 77 (2) ◽  
pp. 304-311 ◽  
Author(s):  
L. D. Silva ◽  
V. C. S. Amaral ◽  
M. C. Vinaud ◽  
A. M. Castro ◽  
H. H. A. Rezende ◽  
...  
Keyword(s):  
Organic Acids ◽  
High Performance ◽  
Chemical Elements ◽  
Essential Element ◽  
Biomphalaria Glabrata ◽  
Control Group ◽  
Limiting Factor ◽  
Energetic Metabolism ◽  
Significant Difference ◽  
Commercial Kits

Abstract Calcium is considered an essential element for the metabolism of aquatic snail Biomphalaria glabrata (Say, 1818), intermediate host of Schistosoma mansoni Sambon, 1907 in Brazil, and represents a limiting factor to its distribution and adaptation to the environment. This study investigated the effect of different concentrations of exogenous CaCO3 on the energetic metabolism of B. glabrata for better understanding the physiological interference of chemical elements dissolved in the environment with the physiology of this species. Sixty-day-old snails were distributed into six groups, five exposed to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) and a control group. The exposure to CaCO3 was assessed over time, with analysis of 15 snails of each group in the following intervals: 1, 14, 21 or 30 days for hemolymph extraction. Concentrations of calcium and glucose in the hemolymph were determined by commercial kits, and organic acids were extracted using an ion exchange column and analyzed by high-performance liquid chromatography. Concentration of calcium in the hemolymph showed no significant difference (p>0.05) from the control group and between the concentrations tested. Concentration of glucose decreased (p<0.05) in the treatments of exposure to 20 and 40 mg/L and increased when exposed to 80 and 100 mg/L CaCO3 compared to control and to other concentrations tested over 30 days. The organic acids pyruvate, oxaloacetate, citrate, succinate, fumarate, beta-hydroxybutyrate and lactate presented increased concentrations, while propionate and acetoacetate, decreased concentrations, when exposed to CaCO3 compared to control. Considering the influence of different periods of exposure to CaCO3, on the 14th day, there were stronger alterations in the metabolism of B. glabrata. In conclusion, exposure to CaCO3 reduced the concentration of glucose, which is metabolized into pyruvate, the final product of glycolysis, and also influenced the energetic metabolism pathways, indicating an aerobic or partially anaerobic functioning.

Anaerobic Capacity of the Upper Arms in Top-Level Team Handball Players

2008 ◽  
Vol 3 (3) ◽  
pp. 251-261 ◽  
Author(s):  
Stylianos N. Kounalakis ◽  
Ioannis A. Bayios ◽  
Maria D. Koskolou ◽  
Nickos D. Geladas
Keyword(s):  
High Performance ◽  
Anaerobic Capacity ◽  
Power Production ◽  
Control Group ◽  
Mean Power ◽  
Muscle Oxygen ◽  
Training Adaptations ◽  
Team Handball ◽  
Physical Education Students ◽  
Significant Difference

Purpose:Handball is a sport with high anaerobic demands in lower body as has been indicated by Wingate test (WT) performed with the legs, but there are no data available concerning power production during a WT performed with the arms in handball players (HndP). Therefore, the purpose of this study was to explore the arm anaerobic profile of HndP during a WT.Methods:Twenty-one elite HndP and 9 physical education students (CON), performed a 30-s arm WT. Power production and muscle oxy-genation were recorded.Results:Peak power (PP) as well as mean power (MP) was higher (P = .017 and 0.03, and ES = 1.00 and 0.86, respectively) for HndP (HndP PP: 7.6 ± 0.8 W·kg−1, CON PP: 6.7 ± 1.1 W·kg−1; HndP MP 5.3 ± 0.6 W·kg−1, CON MP 4.6 ± 0.9 W·kg−1) with no significant difference in fatigue index between the two groups. Muscle oxygen saturation (StO2) declined ~30% with exercise with no differences between groups. During recovery the HndP group had higher StO2 (P = .01, ES= 3.04), total hemoglobin and oxygenated hemoglobin compared with the CON group (P < .01 ES = 3.29 and 0.99, respectively). StO2 returned to resting values in 29.5 ± 2.3 s in HndP, whereas this variable did not recover after 2 min in CON.Conclusions:The arm anaerobic capacity of the HndP was “excellent,” significantly higher than that by the control group. Moreover, HndP exhibited faster recovery of StO2 compared with the control group. The greater power output and the faster muscle reoxygenation of arms in HndP can be attributed to specific training adaptations related to high performance in handball.

scholarly journals Evaluation of Growth and Carcass Characteristics of Broiler Chickens (Cobb 500) Feed on Different Level of Organic Acids Inclusion in Diet at Parwanipur

2019 ◽  
Vol 36 ◽  
pp. 137-147
Author(s):  
A. K. Jha ◽  
Md. H. Azad ◽  
Md.S. N. Ali ◽  
P. Alam ◽  
N. Sheikh ◽  
...  
Keyword(s):  
Weight Gain ◽  
Organic Acids ◽  
Formic Acid ◽  
Broiler Chickens ◽  
Agricultural Research ◽  
Body Weight Gain ◽  
Control Group ◽  
Research Station ◽  
Body Parts ◽  
Significant Difference

 An experiment was carried out to evaluate the effect of different level of organic acids on productive traits, carcass yields and body parts (Thigh, Back, Neck, wings and Breast) of broiler chicken. This experiment was conducted at Regional Agricultural Research Station, Parwanipur, Bara for 41 days to test the effect of organic acids inclusion in broiler feed and its effect on growth performance and carcass study. Altogether 225 day old Cobb-500 broiler birds were procured from Shivam Hatchery, Birgung and divided into 5 treatments with 3 replications (15 birds in each replication) by using completely randomized design. Concentrate feed was purchased from Posak Feed industry, Birgung. Control group (T1) was feed without organic acid inclusion and whereas T2, T3, T4 and T5 groups were fed concentrate mixture mixed with different combination of organic acids @ 400ml/ per 100 kg feed, respectively. Experimental birds were provided adlib amount of grower feed (B1) for 21 days and that after finisher feed (B3) for 20 days and had easily access to drinking water. Feed intake was recorded daily and body weight gain was measured in 7 days interval. Experiment revealed that highest weight gain was observed in T2 (2.6 kg) followed by T3 (2.5) where combination of organic acids were formic acid 65% + propionic acid 35% and formic acid 65% and citric acid 35%, respectively, however, it was not significant among diet groups. From every treatment group each birds were selected for carcass and body parts study. The study showed significant difference (P<0.5) in carcass quantity and body parts of the birds between the treatment groups. The dressing percentage of T1, T2, T3, T4 and T5 were 68.96%, 67.87%, 70.38%, 69.88% and 69.67% respectively. Experiment suggested that further study should be carried out to precise the appropriate level of organic acids inclusion and higher cost benefit ratio.

scholarly journals Daya Hambat Aktivitas Enzim Glukosiltransferase (Gtf) Streptococcus mutans Oleh Ekstrak Temulawak (Curcuma Xanthorrhiza Roxb.)

2017 ◽  
Vol 7 (1) ◽  
pp. 32
Author(s):  
A. Amanda ◽  
Sri Kunarti ◽  
Agus Subiwahjudi
Keyword(s):  
Enzyme Activity ◽  
Streptococcus Mutans ◽  
Antibacterial Agent ◽  
High Performance ◽  
Chlorhexidine Gluconate ◽  
Control Group ◽  
Extract Concentration ◽  
Significant Difference ◽  
Curcuma Xanthorrhiza ◽  
Post Hoc

Background: Streptococcus mutans is a bacteria which has glucosyltransferase (GTF) enzyme and acts as the main agent that causes dental caries. GTF enzyme will convert sucrose into fructose and glucan. Temulawak (Curcuma xanthorrhiza Roxb.) is one of the traditional herbs which has xanthorrhizol, curcumin, flavonoid, tanin, and saponin as an antibacterial agent. Purpose: The purpose of this research is to investigate the effect of temulawak extract (Curcuma xanthorrhiza Roxb.) to the activity of GTF enzyme Streptococcus mutans. Method: This research used 25%, 37,5%, and 50% concentration of  temulawak extract as the treatment, and 0.12% chlorhexidine gluconate as a control. The method of this research consists of three steps; preparing the temulawak extract concentration of 25%, 37,5%, and 50%, preparing the GTF enzyme from the supernatant of Streptococcus mutans, and testing GTF enzyme activity by analyzing the fructose concentration using High Performance Liquid Chromatography (HPLC). Perusal of the fructose area was based on the retention time of fructose. One unit of GTF enzyme activity is defined as the 1 μmol fructose / ml of enzyme / hour. Result: The obtained data then were analyzed by Post-Hoc Tukey (HSD). The result showed a significant difference between each treatment group with the control group (p<0.05). Conclussion: This research concludes that temulawak extract with 25%, 37,5%, and 50% concentrationcan’t inhibit the GTF enzyme activity of Streptococcus mutans.

Pattern of partitioning of aflatoxins from feed to urine and its effect on serum chemistry in Nili-Ravi buffalo heifers

2014 ◽  
Vol 54 (10) ◽  
pp. 1671
Author(s):  
N. Aslam ◽  
Z. M. Iqbal ◽  
H. M. Warriach ◽  
P. C. Wynn
Keyword(s):  
Total Protein ◽  
High Performance ◽  
Clinical Chemistry ◽  
Serum Glucose ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Samples ◽  
Significant Difference ◽  
High Concentrations ◽  
Group D

The objectives of the present study were (1) to monitor the pattern of excretion of aflatoxinM1 in urine after its conversion from aflatoxinB1 and (2) to observe the effects of different levels of aflatoxinB1 in feed on serum concentrations of key metabolites glucose, total protein, cholesterol and urea as indicators of metabolic status. Nili-Ravi buffalo heifers (n = 12) of similar age and weight were randomly distributed to four groups. Animals in Groups A, B and C were offered a contaminated cottonseed cake-based concentrate ration at 0.5%, 1.0% and 1.5% of bodyweight, respectively. Control animals in Group D were fed with aflatoxinB1-free green fodder. Based on the level of contamination of the concentrate ration with aflatoxinB1 (554 µg/kg), Groups A, B and C consumed 953, 2022, 3202 µg of aflatoxinB1 daily. Feed samples were analysed at Romer Laboratories Pty Ltd, Singapore by high performance liquid chromatography. AflatoxinM1 quantification in urine samples was conducted using a competitive enzyme-linked immunosorbent assay with kits supplied by Helica Biosystems, Inc., USA. Serum samples were analysed for concentrations of glucose, total protein, cholesterol and urea using clinical chemistry kits provided by Human diagnostics (HUMAN, Biochemica und Diagnostica mbH, Germany). Carry-over rate of aflatoxinM1 in urine for Groups A, B and C was 15.51%, 15.44% and 14.04% of aflatoxinB1 while there was no detectable aflatoxinM1 in the urine of the control group (D). There was no significant difference in the concentrations of serum glucose, total protein and cholesterol between treatment groups. However, the concentration of serum urea was significantly higher (P < 0.05) in the group offered the highest level of aflatoxinB1-contaminated concentrate. This result suggests that mycotoxicosis may compromise protein metabolism and accretion in affected animals. This leaves open the possibility that high concentrations of aflatoxins in milk may ultimately affect the health status of human milk consumers.

scholarly journals Concentration of essential chemical elements in whole blood and in paranasal sinuses mucosa is related to chronic rhinosinusitis severity in children

2021 ◽  
Vol 6 (4) ◽  
pp. 9-13
Author(s):  
Svetlana I. Alekseenko ◽  
Vladimir V. Dvoryanchikov ◽  
Anatolii V. Skalny ◽  
Igor A. Anikin ◽  
Vadim S. Isachenko ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Whole Blood ◽  
Chemical Element ◽  
Chemical Elements ◽  
Essential Element ◽  
Sinus Surgery ◽  
Control Group ◽  
Healthy Children ◽  
Blood Calcium ◽  
Essential Chemical

Objectives to evaluate the association between essential chemical element levels in whole blood and in pathologic mucosa and chronic rhinosinusitis severity in children. Material and methods. A total of 154 children aged from 6 to 17 years were examined. Among them, 88 children had chronic rhinosinusitis (CRS) and underwent functional endoscopic sinus surgery (FESS). The 66 healthy children were included in the control group. Life quality was assessed using SNOT-20 (Sino-Nasal Outcome Test-20). Endoscopic and computer tomography findings were evaluated using Lund Kennedy and Lund Mackay scales, respectively. The assessment of essential element levels in whole blood samples was performed using inductively-coupled plasma mass-spectrometry. Results. The obtained data demonstrate that total SNOT-20 scores in CRS patients were 68% higher than in controls. At the same time, whole blood calcium, selenium, zinc, and magnesium levels were 6%, 28%, 20%, and 3% lower than the respective control values. The analysis of pathologic mucosa demonstrated that the chemical element contents were reducing in the following order: Ca Mg = Fe Zn Cu Mn Se. However, only whole blood selenium level correlated significantly with its tissue level. In multiple regression models, the whole blood Se level was inversely associated with SNOT-20 and Lund Mackay total scores. Conclusion. Therefore, the obtained data allow to propose the potential role of altered calcium, magnesium, zinc and selenium metabolism in CRS progression.

scholarly journals Serum levels of carotenoids in patients with osteonecrosis of the femoral head are lower than in healthy, community-living people

2018 ◽  
Vol 26 (2) ◽  
pp. 230949901877092 ◽  
Author(s):  
Toshiaki Okura ◽  
Taisuke Seki ◽  
Koji Suzuki ◽  
Naoki Ishiguro ◽  
Yukiharu Hasegawa
Keyword(s):  
Femoral Head ◽  
High Performance ◽  
Serum Levels ◽  
Community Living ◽  
Control Group ◽  
Health Examination ◽  
Total Carotenoids ◽  
Significant Difference ◽  
Healthy Community ◽  
Β Carotene

Purpose: Oxidative stress is closely associated with the pathogenesis of nontraumatic osteonecrosis of the femoral head (ONFH). This study aimed to determine whether the serum levels of antioxidant nutrients were decreased in patients with ONFH. Methods: We analyzed the serum levels of antioxidant nutrients in 39 patients with ONFH (ONFH group) and 78 age- and gender-matched healthy people (control group) who voluntarily participated in the Yakumo study, which is a comprehensive health examination program. We measured and compared the serum levels of α-tocopherol (vitamin E) and total carotenoids, including zeaxanthin/lutein, β-cryptoxanthin, lycopene, α-carotene, and β-carotene, in the ONFH and control groups using high-performance liquid chromatography. Results: The mean serum levels of total carotenoids were significantly lower in the ONFH group than in the control group (2.36 ± 1.26 and 3.79 ± 2.36 µmol/l, respectively, p < 0.001). However, no significant difference was found in α-tocopherol between the two groups (26.37 ± 6.90 µmol/l in the ONFH group and 26.24 ± 6.28 µmol/l in the control group, p = 0.920). Among each carotenoid, the serum levels of zeaxanthin/lutein, lycopene, and β-carotene were significantly lower in the ONFH group than in the control group ( p < 0.001). Conclusions: The serum levels of carotenoids were lower in patients with ONFH than in healthy, community-living people. This result suggests that carotenoids may be related to the pathogenesis of ONFH.

scholarly journals The portrait of neem leaves-based high performance wound healing activity on zebrafish

2021 ◽  
Vol 27 (1) ◽  
pp. 23-27
Author(s):  
Nour Athiroh ◽  
Ari Hayati ◽  
Istirochah Pudjiwati ◽  
Ahmad Taufiq ◽  
Nurul Jadid Mubarakati
Keyword(s):  
Wound Healing ◽  
High Performance ◽  
Healing Process ◽  
Vital Role ◽  
Control Group ◽  
Wound Healing Process ◽  
Physiological Factors ◽  
Neem Leaves ◽  
Significant Difference ◽  
Speed Up

This study was designed to evaluate the role of sliced and dropping models of neem leaves (Azadirachta indica) and environmental factors on zebrafish wound shrinkage. This study employed two treatment models: neem leaf slices and drops model. The treatment in the neem leaf slice model was the control group (fish was injured without neem leaf slices treatment, and G1-G3 of each fish was injured at 0.3 cm + 0.5, 1, and 2 g. Treatment in the neem leaf drop model was control group (fish was injured without neem leaves drops, and G1-G3 of each fish was injured at 0.3 cm + 1, 2, 4 drops. Findings suggest that there is a significant difference between the control group by treating G1, G2, and G3 on the shrinkage of zebrafish wound area both in the neem leaf slice and drop models, but G1 was not significantly different from G2 and G3, as well as G2 and G3 both in the neem leaf slice and drop model. Neem leaves contain nutrients that play a vital role in the formation of collagen and the formation of new capillaries to help speed up the wound healing process. The healing process involves the dynamic interaction of physiological factors.

scholarly journals Nutritional status of patients with biliary atresia and autoimmune hepatitis related to serum levels of vitamins A, D and E

2009 ◽  
Vol 46 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Margareth L. G. Saron ◽  
Helena T. Godoy ◽  
Gabriel Hessel
Keyword(s):  
Vitamin E ◽  
Nutritional Status ◽  
Biliary Atresia ◽  
Autoimmune Hepatitis ◽  
High Performance ◽  
Reference Group ◽  
Serum Levels ◽  
Skinfold Thickness ◽  
Control Group ◽  
Significant Difference

CONTEXT: Chronic liver disease may induce to malabsorption of lipids and fat-soluble vitamins, leading to injury of nutritional status. OBJECTIVES: To evaluate the nutritional status of pediatric-age patients with autoimmune hepatitis and biliary atresia related to serum levels of vitamins A, D and E and the disease severity. METHODS: This controlled transverse study, evaluated the patients with autoimmune hepatitis and biliary atresia and a reference group paired by sex and age. The patients underwent anthropometric evaluation, alimentary inquiry and determination of serum levels of vitamins A, D and E by high performance liquid chromatography. The Mann-Whitney test, Spearman correlation coefficients and variance analysis (ANOVA) were utilized for data treatment, regarding significant difference if P<0.05. RESULTS: The highest nutritional deficit was observed in patients with biliary atresia, mainly with cholestasis. The serum levels of vitamins A and E for the reference group changed as a function of age. The serum levels of vitamins A, D and E were higher in reference group than in patients with biliary atresia and autoimmune hepatitis together or separately. There were not difference in the serum levels of vitamins A, D and E between biliary atresia groups with cholestasis and without cholestasis. It was verified correlation between weight/age, triceps skinfold thickness, subscapular skinfold thickness, midarm circumference, midarm fat area values and vitamin A serum levels, as well as between all anthropometric indicators and vitamin E in patients with autoimmune hepatitis and biliary atresia. CONCLUSION: The patients with biliary atresia and cholestasis presented the highest nutritional injury. The patients with biliary atresia and autoimmune hepatitis presented lower serum levels of vitamins A, D and E that in control group. There is a directly proportional correlation between vitamin serum levels, mainly vitamin E, and all anthropometric variables of biliary atresia and autoimmune hepatitis groups.

scholarly journals The Effect of Recently Developed Synbiotic Preparations on Dominant Fecal Microbiota and Organic Acids Concentrations in Feces of Piglets from Nursing to Fattening

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1999
Author(s):  
Agnieszka Chlebicz-Wójcik ◽  
Katarzyna Śliżewska
Keyword(s):  
Fatty Acids ◽  
Organic Acids ◽  
Intestinal Microbiota ◽  
High Performance ◽  
Negative Impact ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Plate Count ◽  
Control Group ◽  
Chain Fatty Acids

The study was conducted to determine the influence of newly elaborated synbiotic preparations on piglets’ intestinal microbiota and its metabolism. Animals were distributed among six experimental groups, in reference to used feed supplements, namely, synbiotics (A, B, or C) or commercially available probiotics (BioPlus 2B®, Chr. Hansen A/S, Horsholm, Denmark or Cylactin® LBC, DSM Nutritional Products Ltd., Kaiseraugst, Switzerland), or its absence (control group). Until the 29th day of life, piglets were breastfed by sows, whose feed was supplemented, and fecal samples were collected at the 7th and 28th day of piglets’ life. After weaning of the piglets, the research was continued until the 165th day of the pigs’ life. The area of this work included the analysis of the piglets’ dominant fecal microbiota by the plate count method. Moreover, high-performance liquid chromatography analysis (HPLC) was applied to establish variations in the concentrations of organic acids, namely, lactic acid, short-chain fatty acids (SCFAs), and branched-chain fatty acids (BCFAs). It was observed that synbiotics have a more significant beneficial effect on the intestinal microbiota of piglets and their metabolism, and therefore their health, in comparison to commercial probiotics used individually. Moreover, synbiotic preparations prevent the negative impact of weaning on piglets’ microbial population in the gastrointestinal tract, which could reduce the occurrence of diarrhea.

scholarly journals High-performance inulin and oligofructose prebiotics increase the intestinal absorption of iron in rats with iron deficiency anaemia during the growth phase

2011 ◽  
Vol 108 (6) ◽  
pp. 1008-1016 ◽  
Author(s):  
Karine de Cássia Freitas ◽  
Olga Maria Silvério Amancio ◽  
Mauro Batista de Morais
Keyword(s):  
Intestinal Absorption ◽  
High Frequency ◽  
High Performance ◽  
Fe Deficiency ◽  
Control Group ◽  
Control Groups ◽  
Deficiency Anaemia ◽  
Significant Difference ◽  
Group 2 ◽  
And Control

Considering the high frequency of anaemia due to Fe deficiency, it is important to evaluate the effects of prebiotics on the absorption of Fe. The aim of the present study was to evaluate the effects of high-performance (HP) inulin, oligofructose and synergy1 during recovery from anaemia in rats through the intestinal absorption of Fe, food intake, body growth, caecal pH and weight of the intestine. Wistar rats (n 47) were fed with rations of AIN93-G with no Fe to induce Fe deficiency anaemia. At 36 d of life, anaemic rats were divided into four groups: (1) the HP inulin group; (2) the synergy1 group; and (3) the oligofructose group, all with 100 g of the respective prebiotic per kg of ration; and (4) a control group, in which the prebiotic was replaced by maize starch. Then, 25 mg of elemental Fe/kg of ration was added to all rations to allow recovery from anaemia. The final values of Hb in the HP inulin, synergy1, oligofructose and control groups were, respectively: 98 (94–99); 83 (81–92); 100 (90–114); 77 (72–81) g/l, with a statistically significant difference (P ≤ 0·001) between the oligofructose and control groups and the HP inulin and control groups. The four groups had an increase in weight and body length and had similar consumption of rations. The intestinal weight and caecal pH were significantly different between the groups that consumed prebiotics and the control group. HP inulin and oligofructose increased the intestinal absorption of Fe in rats.

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