scholarly journals UJI DAYA HAMBAT EKSTRAK ETANOL DAUN SRIKAYA (Annona squamosa L.) TERHADAP PERTUMBUHAN Staphylococcus aureus SECARA IN VITRO

2019 ◽  
pp. 50-56
Author(s):  
Vector Stephen Dewangga ◽  
Ardy Prian Nirwana
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Inhibition Test ◽  
Test Method ◽  
Diffusion Method ◽  
Annona Squamosa ◽  
Post Test ◽  
Post Hoc ◽  
One Way Anova ◽  
Range Test

Staphylococcus aureus merupakan salah satu bakteri penyebab bakteremia. Bakteremia dapat diatasi dengan menggunakan pengobatan tradisional yang lebih aman, salah satu alternatifnya dengan menggunakan daun Annona squamosa L. Tujuan penelitian adalah untuk mengetahui daya hambat ekstrak etanol daun A. squamosa L. dan mengetahui konsentrasi optimal dari ekstrak etanol daun A. squamosa L. dalam menghambat pertumbuhan S. aureus. Penelitian menggunakan desain analitik eksperimental dengan pendekatan Post Test with Control. Penelitian dilakukan di Laboratorium Bakteriologi STIKES Nasional Surakarta pada bulan Februari hingga Mei 2018. Sampel penelitian adalah ekstrak etanol daun A. squamosa L. dengan konsentrasi 12,5%, 25% dan 50%. Teknik sampling yang digunakan adalah quota sampling. Hipotesis dilakukan dengan One-way Anova, dilanjutkan uji Post Hoc dengan metode Duncan’s Multiple Range Test. Dari penelitian dijumpai diameter zona radikal 8,17 mm pada konsentrasi 12,5%; 9,57 mm pada konsentrasi 25%, dan 10,58 mm pada konsentrasi 50%. Uji Anova diperoleh hasil signiÞ kan yang artinya terdapat beda nyata diantara semua perlakuan. Ekstrak etanol daun A. squamosa L. mampu menghambat pertumbuhan S. aureus, namun belum seoptimal ciprofloxacin. Kata kunci: Uji daya hambat, ekstrak etanol, daun Annona squamosa L., Staphylococcus aureus, ciprofloxacin Staphylococcus aureus is one of bacteria that can cause bacterimia. Bacterimia can be prevented with traditional medicine which is safer, one of which is using the leaves of Annona squamosa L. The purpose of this research is to discover the inhibition test of ethanol extract of A. squamosa L. leaves and knowing the optimal concentration of ethanol extracts with A. squamosa L. leaves in inhibiting the growth of S. aureus. This study is an analytic experimental design and post test with control. The research was done during February to May 2018 at Bacteriological Laboratory of STIKES Nasional. The sample of this research is ethanol extract of A. squamosa L. leaves in 12,5%, 25%, and 50% concentration. Hypothesis test is done with One-way Anova, post hoc test followed by Duncan’s Multiple Range Test method. The result of this study has been found radical zone diameter in 12,5%, 25%, 50% concentration are 8,77 mm, 9,57 mm, 10,58 mm. The result Anova test is found to be signiÞ cant, which means there is real difference between all variance treatment. Ethanol extract of A. squamosa L. leaves has inhibition power against the growth of S. aureus with well diffusion method. Although there is no concentration more optimal than positive control (ciprofloxacin). Keywords: Inhibition test, ethanol extract, Annona squamosa L. leaves, Staphylococcus aureus, ciprofloxacin.

scholarly journals EFEKTIFITAS EKSTRAK DAUN SRIKAYA (Annona squamosa L) TERHADAP PERTUMBUHAN Staphyloccoccus aureus SECARA IN VITRO

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Andita Fitriani ◽  
Erni Setiyorini ◽  
Farach Khanifah
Keyword(s):  
Staphylococcus Aureus ◽  
Control Group ◽  
Annona Squamosa ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Post Hoc ◽  
One Way Anova

Pendahuluan : Bakteri Staphylococcus aureus merupakan bakteri yang paling sering menyebabkan infeksi. Pemberian antibiotik merupakan upaya pengendalian terhadap infeksi yang dapat menyebabkan resisten. Bakteri Staphylococcus aureus telah resisten terhadap antibiotik ampisilin, amoksisilin-asam klavulanat, amoksisilin, penisilin G, sulbenisilin, kloramfenikol dan siprofloksasin sehingga penanganan terhadap infeksi Staphylococcus aureus relatif sulit. Daun Srikaya diketahui mengandung senyawa flavonoid, saponin dan tannin yang memiliki efek antimikroba. Metode Penelitian : Dalam penelitian ini ditentukan Kadar Hambat Minimum (KHM) dengan menggunakan metode dilusi padat.Penelitian ini merupakan penelitian eksperimen analitik dengan post test only control group design. Sampel dalam penelitian ini adalah bakteri Staphylococcus aureus yang merupakan stok kultur milik Balai Besar Laboratorium Kesehatan (BBLK) Surabaya. Variabel independen dalam penelitian ini adalah ekstrak daun srikaya (Annona squamosa L.) dengan konsentrasi 3%, 6%, 12% dan 24%. Variabel dependen dalam penelitian ini adalah bakteri Staphylococcus aureus. Data dianalisis dengan uji one way ANOVA dilanjutkan uji Post Hoc LSD dengan nilai probabilitas (p)<0,05. Hasil Penelitian : Hasil penelitian menunjukkan bahwa terdapat penurunan jumlah koloni bakteri Staphylococcus aureus yang berbanding terbalik dengan peningkatan konsentrasi ekstrak daun srikaya mulai dari konsentrasi 3% hingga 24%.Kesimpulan : Kesimpulan pada penelitian ini yaitu ekstrak daun srikaya mempunyai efek antimikroba terhadap pertumbuhan Staphylococcus aureus dengan KHM terletak pada konsentrasi dua kali lipat dari konsentrasi 24%. Saran : Sebagai referensi bakteri yang dapat menyebabkan infeksi dan dapat menangsninys dengan antimikroba alami yang minimefek samping disbanding dengan BKOKata Kunci: Antimikroba, Ekstrak Daun Srikaya, , Kadar Hambat Minimum (KHM), Staphylococcus aureus

scholarly journals COMPARISON OF EFFECTIVENESS OF BASIL LEAVES ETHANOL EXTRACT WITH GARLIC ON STAPHYLOCOCCUS AUREUS BACTERIA

Biospecies ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 8-14
Author(s):  
Nico Marcelino ◽  
Elisabeth Chindy Clara ◽  
Kania Indryani Fauhan ◽  
Alfi Syahri Ramadhana ◽  
I Nyoman Echrich Lister
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Sample Collection ◽  
Disk Diffusion Method ◽  
Post Test ◽  
Comparison Of Effectiveness ◽  
Post Hoc

Writing the results of the study was conducted to assess the effectiveness comparison of the ethanol extract of basil leaves with garlic with a concentration of 5%, 10%, 15% of each extract against the growing power of the Staphylococcus aureus bacteria. Staphylococcus aureus bacteria are gram-positive, cocci-shaped bacteria that live in colonies. These bacteria are normal flora in the human body that can harm humans. This study uses an experimental method with a post-test only design and the sample collection uses purposive sampling. The effectiveness test was carried out using the disk diffusion method, where the paper disc would be moistened with basil leaves ethanol extract of each concentration, is 5%, 10%, 15% and so was the ethanol extract of garlic. The results of the data obtained from this study continued with the Oneway-Anova test which will be continued with the Post-Hoc test, wherein the test there were significant differences from each treatment given. In bacteria treated with ethanol extract 15% basil leaves against Staphylococcus bacteria aureus, the effectiveness of the inhibition zone was found to be 9.2 mm in diameter where the inhibition zone was below the ethanol extract of 15% garlic which was 22.8 mm. Ethanol extract of 15% garlic has a good antimicrobial effect compared with ethanol extract of basil leaves 15% against Staphylococcus aureus bacteria.

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KALANDUYUNG (Guazuma ulmifolia Lam.) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS DENGAN METODE DIFUSI CAKRAM (KIRBY-BAUER)

2018 ◽  
Vol 17 (2) ◽  
pp. 136-143
Author(s):  
Adelgrit Trisia ◽  
Regina Philyria ◽  
Angeline Novia Toemon
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Lung Parenchyma ◽  
Positive Control ◽  
Diffusion Method ◽  
Control Group ◽  
Control Group Design ◽  
Guazuma Ulmifolia ◽  
One Way Anova

Pneumonia is an infectious lung parenchyma, respiratory bronchioles, and alveolus, caused by Staphylococcus aureus. Kalanduyung (Guazuma ulmifolia Lam.) is an herb plant from Central Borneo which people believe has many benefits, which one is curing pneumonia. To identify the antibacterial activity from the ethanol extract of Kalanduyung leaves to the Staphylococcus aureus growth. This research was experimental with posttest-only control group design. Ethanol extract of Kalanduyung leaves in 20%, 40%, 60%, and 80% concentrations were tested for antibacterial capability against Staphylococcus aureus with Kirby Bauer disc diffusion method, with Amoxicillin as the positive control and 10% Dimethyl Sulfoxide. This data was statistically analyzed with One Way Anova. Ethanol extract of Kalanduyung leaves can inhibit Staphylococcus aureus with each inhibition zone diameter was 6.875 mm, 8.5 mm, 10.175 mm, and 14.925 mm. The ethanol extract of Kalanduyung leaves can inhibit the growth of Staphylococcus aureus bacteria.

scholarly journals Pengaruh Senam Nifas terhadap Penurunan Tinggi Fundus Uteri dan Lochea pada Ibu Pasca Bersalin yang Mendapatkan Inisiasi Menyusu Dini dan Mobilisasi Dini

2020 ◽  
Vol 19 (03) ◽  
pp. 126-133
Author(s):  
Siska Toloan Toloan ◽  
Harimat Hendarwan
Keyword(s):  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Group Design ◽  
Consecutive Sampling ◽  
Post Test ◽  
Post Hoc ◽  
One Way Anova

Masa nifas adalah hal sangat penting untuk diperhatikan guna untuk menurunkan angka kematian ibu dan bayi di Indonesia. Penelitian ini bertujuan untuk Mengetahui Pengaruh Senam Nifas Terhadap Penurunan Tinggi Fundus Uteri Dan Lochea Pada Ibu Pasca Bersalin Yang Mendapatkan Inisiasi Menyusu Dini Dan Mobilisasi Dini Di Praktek Bidan Mandiri Kota Depok Tahun 2019. Penelitian ini menggunakan penelitian kuantitatif yang menggunakan desain penelitian Quasy Exsperimental dengan pendekatan post test only control group design. Populasi yang diambil yaitu 65 ibu pasca bersalin. Sampel diambil dengan metode consecutive sampling besar sampel ditentukan dengan menggunakan rumus Federer setiap variabel bebas terdiri atas 3 kelompok dan setiap kelompok sebanyak 9 sampel sehingga jumlah total sampel 27 ibu pasca bersalin. Pengumpulan data berupa data primer dan uji yang digunakan yaitu uji One Way Anova. Dapat dilihat dari nilai signifikan uji One Way Anova pada penurunan tinggi fundus uteri hari pertama sampai hari kesepuluh dengan nilai signifikan yaitu p-value < 0,05 yang artinya H0 ditolak atau ada perbedaan secara signifikan dan dilihat. Hasil post hoc test tidak terdapat perbedaan signifikan pada kelompok inisiasi menyusu dini, inisiasi menyusu dini dan mobilisasi dini dan kelompok inisiasi menyusu dini, mobilisasi dini dan senam nifas terhadap penurunan tinggi fundus uteri.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

scholarly journals UJI EFEK ANTI BAKTERI EKSTRAK BUNGA CENGKEH TERHADAP BAKTERI Streptococcus mutans SECARA IN VITRO

e-GIGI ◽  
2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Juvensius R. Andries ◽  
Paulina N. Gunawan ◽  
Aurelia Supit
Keyword(s):  
Streptococcus Mutans ◽  
Control Group ◽  
Antibacterial Effects ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Clove Extract ◽  
Post Hoc ◽  
One Way Anova

Abstrak: Minyak cengkeh berguna sebagai antibakteri alami. Minyak esensial dari cengkeh mempunyai fungsi anestetik dan antimikrobial. Zat yang terkandung dalam cengkeh yang bernama eugenol dapat membunuh bakteri termasuk bakteri yang resisten terhadap antibiotika, salah satunya adalah bakteri Streptococcus mutans. Bakteri ini merupakan mikroorganisme penyebab utama terjadinya karies. Tujuan dari penelitian ini yaitu untuk mengetahui efek antibakteri ekstrak cengkeh terhadap bakteri Streptococcus mutans secara in vitro. Penelitian ini merupakan penelitian eksperimental menggunakan post test only control group design. Penelitian ini menggunakan bahan coba ekstrak cengkeh dengan konsentrasi 40%, 60%, dan 80%, Ciprofloxacin, aquades dengan pengulangan sebanyak lima kali. Data dikumpulkan dan dianalisis dengan one-way ANOVA dan post-hoc uji LSD ( = 0,05). Berdasarkan hasil uji statistik penelitian uji efek antibakteri ekstrak cengkeh terhadap bakteri streptococcus mutans secara in vitro, dapat disimpulkaan bahwa ekstrak cengkeh memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans secara in vitro. Hasil uji lanjut post-hoc uji LSD menunjukan daya hambat ekstrak cengkeh 40%, 60%, 80%, lebih kecil (p<0,05) dalam menghambat Streptococcus mutans secara in vitro dibandingkan Ciprofloxacin. Kata Kunci: Ekstrak cengkeh, Streptococcus mutans.   Abstract: Clove oil is useful as a natural antibacterial agent, essential oil of clove has anesthetic and antimicrobial effect. Substances contained in clove called eugenol can kill bacteria including antibiotic resistant bacteria, one of which is the bacteria Streptococcus mutans. This bacteria is a major cause for caries. The purpose of this study was to mengetahui clove extrack antibacterial effects againts Streptococcus mutans bacteria in vitro. This study is an experimental study using a post test only control group design. This research try using clove extract with a concentration of 40%, 60%, and 80%, Ciprofloxacin, aquades repetition five times. Data collected and analyzed by one-way ANOVA and post-hoc LSD test (α = 0.05). Based on the results of the statistical test to test the effects of anti-bacterial research clove extracts against Streptococcus mutans bacteria in vitro, can disimpulkaan that clove extracts have antibacterial effects in inhibiting the growth of Streptococcus mutans bacteria in vitro.further test result post-hoc LSD test shoved its inhibitory clove extract 40%, 60%, 80% smaller (p<0,05)in hibiting Streptococcus mutans in vitro compared Ciprofloxacin. Keywords: clove extract, Streptococcus mutans

scholarly journals Uji Daya Hambat Ekstrak Etanol Batang Saluang Belum (Luvunga sarmentosa Kurz) Terhadap Bakteri Propionibacterium acnes

2021 ◽  
Vol 8 (1) ◽  
pp. 65
Author(s):  
Rezqi Handayani ◽  
Nurul Qamariah ◽  
Muhammad Izmiansyah
Keyword(s):  
Secondary Metabolites ◽  
Inhibitory Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Inhibition Test ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Percolation Method

Saluang Belum (Luvunga sarmentosa Kurz) adalah tumbuhan yang dikenal sebagai obat tradisional di Kalimantan Tengah. Batang L. sarmentosa Kurz dipercayai memiliki manfaat secara empiris sebagai antioksidan yang dimanfaatkan sebagai anti-aging (anti penuaan dini). Batang L. sarmentosa Kurz mengandung metabolit sekunder yaitu tanin, saponin, steroid dan flavonoid yang diduga memiliki aktivitas sebagai antibakteri. Berdasarkan khasiat empiris dan kandungan metabolit sekunder yang ada dalam tumbuhan L. sarmentosa Kurz, maka dilakukan penelitian tentang aktivitas daya hambat ekstrak etanol tumbuhan ini terhadap bakteri P. acnes yang merupakan salah satu bakteri penyebab jerawat. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas daya hambat ekstrak etanol batang L. sarmentosa Kurz terhadap bakteri P.acnes. Metode penelitian yang dilakukan pada penelitian ini dimulai dari pengambilan simplisia, pembuatan ekstrak etanol dengan metode perkolasi dan uji daya hambat dengan menggunakan metode Disk Difusion yang menggunakan kontrol positif antibiotik klindamicin. Analisis data dilakukan dengan membandingkan lebar zona hambat dari ekstrak etanol batang L. sarmentosa Kurz  dengan kategori penghambatan antimikroba klindamisin berdasarkan diameter zona hambat dari Clinical and Laboratory Standards Institute.  Hasil uji daya hambat didapatkan zona hambat ekstrak etanol batang Saluang Belum pada semua konsentrasi 0,5%, 1%, 5%, 10% dan 15% secara berturut-turut yaitu  Intermediate, Resistant, Intermediate, Susceptible dan Susceptible dan hasil dari zona hambat klindamicin pada semua konsentrasi 0,5%,1%,5%,10%,dan 15% adalah susceptible. Simpulan dari penelitian ini adalah ekstrak etanol batang L. sarmentosa Kurz mempunyai kemampuan dalam menghambat pertumbuhan bakteri P.acnes.           Kata Kunci: Obat Tradisional, Jerawat, Uji Daya Hambat, Propionibacterium acnes, Batang Saluang Belum Saluang belum (Luvunga sarmentosa Kurz) is one of traditional medicine in Central Kalimantan. The stem of L. sarmentosa Kurz is believed to have empirical benefits as an antioxidant which is used as anti-ageing (anti-ageing). The stem of L. sarmentosa Kurz contains secondary metabolites, namely tannins, saponins, steroids and flavonoids which are thought to have antibacterial activity. Based on the empirical properties and the content of secondary metabolites in L. sarmentosa Kurz, a study was conducted on the inhibitory activity of this plant's ethanol extract against P. acnes, which is one of the bacteria that causes acne. The purpose of this study was to determine the inhibitory activity of the ethanol extract of L. sarmentosa Kurz stem against P.acnes bacteria. The research method carried out in this study started from taking simplicia, making ethanol extract using the percolation method and the inhibition test using the disk diffusion method which used a positive control of the clindamycin antibiotic. Data analysis was performed by comparing the inhibition zone width of the ethanol extract of L. sarmentosa Kurz stem with the clindamycin antimicrobial inhibition category based on the inhibition zone diameter of the Clinical and Laboratory Standards Institute. The results of the inhibition test obtained the inhibition zone of Saluang Belum stem ethanol extract at all concentrations of 0.5%, 1%, 5%, 10% and 15% respectively, Intermediate, Resistant, Intermediate, Susceptible and Susceptible and the results of the inhibition zone. Clindamycin at all concentrations of 0.5%, 1%, 5%, 10%, and 15% are susceptible. This research concludes that the ethanol extract of L. sarmentosa Kurz stem can inhibit the growth of P.acnes bacteria.

scholarly journals The Antibacterial Effectiveness of Red Ginger (Zingiber Officinale Roscoe) Essential Oil in Inhibiting The Growth of Staphylococcus Aureus and Streptococcus Mutans

2021 ◽  
Vol 7 (2) ◽  
pp. 364-373
Author(s):  
Shavilla Lukita ◽  
Winda Khosasi ◽  
Chandra Susanto ◽  
Florenly
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Ginger Extract ◽  
Zingiber Officinale Roscoe ◽  
Red Ginger ◽  
Post Hoc

Red ginger extract has a category strong antibacterial effect on Staphylococcusaureus and Streptococcus mutans. Red ginger essential oil has the potential forstronger inhibition. This study aims to compare the antibacterial effectiveness of redginger essential oil against Staphylococcus aureus and Streptococcus mutans. Thedesign of this study was a laboratory experimental design with a factorial completelyrandomized design. The red ginger used in this study was proven to be a species ofZingiber officinale Roscoe. The production of essential oils in this study uses thesteam distillation method. The content of secondary metabolites in red ginger wastested quantitatively by the GC-MS method. Determination of antibacterial activityusing the disc diffusion method. The data were processed using the SPSS 21.0program. The normality of data distribution was tested with the Shapiro-Wilk test,followed by one-way ANOVA, Levene's test, and the Tukey HSD Post Hoc Test. Theresults of the antibacterial test of red ginger essential oil against Staphylococcusaureus (21.21mm ± 0.315) and Streptoococcus mutans (23.43mm ± 0.189) provedthat the inhibition power of the category was very strong at a concentration of 75%.

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