scholarly journals Antibacterial Effects of a 940 nm Diode Laser With/ Without Silver Nanoparticles Against Enterococcus faecalis

2021 ◽  
Vol 12 (1) ◽  
pp. e73-e73
Author(s):  
Seyedeh Sareh Hendi ◽  
Maryam Shiri ◽  
Banafsheh Poormoradi ◽  
Mohammad Yousef Alikhani ◽  
Saeid Afshar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Diode Laser ◽  
Sodium Hypochlorite ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Antibacterial Effects ◽  
Group 4 ◽  
Group 1

Introduction: The final goal of root canal therapy is to remove of the most bacteria from the root canal. This study aimed at comparing the antibacterial effects of a diode laser with a wavelength of 940nm and silver nanoparticles and the synergic effects of both techniques on Enterococcus faecalis. Methods: Ninety single-rooted human teeth were decoronated and prepared with rotary files. The samples were irrigated with sodium hypochlorite and EDTA17%. Then they were autoclaved and contaminated with E. faecalis suspension (1.5 × 108 CFU/mL) for 21 days. The samples were fixed in a microtube and were randomly divided into 4 experimental (n=20) groups and a negative control group (n=10) as follows: Group 1: hypochlorite sodium 5%, Group 2: silver nanoparticle, Group 3: diode laser, and Group 4: diode laser and silver nanoparticle. The samples were obtained from dentin chips before and after the intervention. The data were analysed using the Kruskal-Wallis nonparametric test. Furthermore, alterations in bacterial colonies were entered using the Wilcoxon signed ranks test (α=0.05). Results: There was a significant decrease in colony counts for all groups after interventions (P value<0.05). Also, all groups showed more reductions in colony counts compared with the negative control group (P value <0.004). There was a significant reduction for group 1 in comparison with other groups (P value <0.001) and this group had an extreme decrease of colony counts (RCC=100%). There was an important differential between silver nanoparticles and diode laser groups in bacterial counts (P value<0.001) and silver nanoparticles (RCC=83.15%) had more efficiency than the diode laser (RCC=41/33%). RCC of group 4 was 68/52%. Conclusion: Followed by sodium hypochlorite 5%, silver nanoparticles were the most effective antibacterial substances. The 940 nm laser diode had less antibacterial effect compared to its use with silver nanoparticles.

scholarly journals Effect of different periodontal ligament simulating materials on the incidence of dentinal cracks during root canal preparation

2018 ◽  
Vol 12 (3) ◽  
pp. 196-200
Author(s):  
Ajita Rathi ◽  
Prateeksha Chowdhry ◽  
Mamta Kaushik ◽  
Pallavi Reddy ◽  
Roshni Roshni ◽  
...  
Keyword(s):  
Root Canal ◽  
Periodontal Ligament ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Root Canal Preparation ◽  
Impression Material ◽  
Group 1

Background. The present study was undertaken to evaluate the incidence of dentinal cracks during root canal preparation with different periodontal ligament simulating materials in vitro. Methods. Seventy freshly extracted human mandibular first premolars were selected and divided into 7 groups in terms of simulating material: group 1: polyether impression material; group 2: polyvinyl acetate adhesive; group 3: polyvinyl siloxane impression material; group 4: cyanoacrylate adhesive; group 5: epoxy resin adhesive; group 6: positive control, without any periodontal ligament simulation; and group 7: negative control, where neither a periodontal ligament simulating material was used nor canal preparation was carried out. Root canal preparation was carried out in all the groups followed by sectioning of roots at 3 mm, 6 mm and 9 mm. The sections were evaluated under a stereomicroscope at ×2.5 for the presence or absence of cracks. Chi-squared test was used to compare the appearance of defective roots between the different experimental groups. Results. The least number of cracks were found in the negative control group, followed by group 1 where polyether impression material was used for periodontal ligament simulation. The difference was significant with a P-value of 0.002 for coronal sections. Conclusion. Under the limitation of the present study, polyether and polyvinyl siloxane (light body) can both be used for simulation of periodontal ligament.

scholarly journals Replacement of vitamin-mineral premix by Spirulina and its effect on the performance of broiler

2018 ◽  
Vol 2 ◽  
pp. 39
Author(s):  
Shahinur Nahar Moury ◽  
Md. Touhiduzzaman Sarker ◽  
Adhita Sri Prabakusuma ◽  
Md. Imrul Hasan Russel ◽  
Md. Shahidul Islam
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Group 4 ◽  
Group 6 ◽  
Group 5 ◽  
And Control ◽  
Group 1

The main purpose of this study was to explore the effects of Spirulina (Spirulina platensis) as a feed supplement by replacing Vitamin-Mineral Premix on the performance of broiler. The study explored that final body weight was 1039, 1070, 1044, 1065, 1117 and 893 g/bird in group 1, 2, 3, 4, 5 and 6 where significantly (p<0.5) higher weight observed in Spirulina group (5) but lower in negative control group (6). Feed intake observed more or less similar in all groups (1483±24g; p>0.5). Feed conversion ratio (kg FI/kg LWG) observed significantly different like 1.72, 1.65, 1.69, 1.62, 1.61 and 1.99 in group 1, 2, 3, 4, 5 and 6 respectively (p<0.5) where higher in negative group (6) and control group (1), but is lowest and similar in 75% Spirulina group (4) and highest (100%) Spirulina group (5). %Ash of different dietary groups were 46.35, 46.54, 48.93, 49.93, 55.07 and 46.92 of group 1, 2, 3, 4, 5 and 6 respectively. The %ash of bone was gradually increased among dietary groups and the best result was obtained in 100% Spirulina group 5 (55.07). It also revealed that diets containing no vitamin-mineral premix with 100% Spirulina improve the performance of broiler. The findings suggest that poultry farmer can use spirulina as a feed item for broiler for more yield.

Deproteinizing Agents as an Effective Enamel Bond Enhancer-An in Vitro Study

2017 ◽  
Vol 41 (4) ◽  
pp. 280-283 ◽  
Author(s):  
Pallavi Hasija ◽  
Vinod Sachdev ◽  
Shivani Mathur ◽  
Rishi Rath
Keyword(s):  
Bond Strength ◽  
Sodium Hypochlorite ◽  
Shear Bond Strength ◽  
Testing Machine ◽  
Acid Etching ◽  
Control Group ◽  
P Value ◽  
Group 4 ◽  
Significant Difference ◽  
Group 1

Objective: The aim of this study was to compare the effect of different deproteinizing agents on shear bond strength of composite to primary teeth enamel. Study Design: Forty sound primary molars divided in 4 groups of 10 teeth each. In control group 1, enamel was etched for 60 seconds with 37% phosphoric acid and rinsed with water. Group 2: after acid etching deproteinizing agent 5 % sodium hypochlorite was applied for 60 seconds and rinsed. Group 3: after acid etching deproteinizing agent papain gel was applied for 60 seconds and rinsed. Group 4: after acid etching deproteinizing agent bromelain gel applied for 60 seconds and rinsed. Following this, bonding agent was applied to treated enamel surface and composite resin disc were build. Samples were then tested for shear bond strength using Universal Testing Machine. Results: Mean SBS was highest for group 4 and lowest for group 1. No statistically significant difference (p value &gt;0.05) was found between all the four groups. Conclusion: Among deproteinizing agents, deproteinization when carried out with bromelain gel and sodium hypochlorite showed effective bond strength as compared to papain.

scholarly journals PENGARUH PEMBERIAN SUSU BEKATUL TERHADAP KONSENTRASI KOLESTEROL DAN TRIGLISERIDAPADA TIKUS PUTIH WISTAR DIABETES

2019 ◽  
Vol 12 (02) ◽  
Author(s):  
Husnul Khatimah ◽  
Saifuddin Sirajuddin ◽  
Zainal Zainal
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Group 4 ◽  
White Rats ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

ABSTRACT In diabetics there is usually an increase in free fatty acids in the blood and increases the concentration of cholecerols and triglycerides, this condition is called diabetes dyshidemia. This study aimed to find out at the effect of bran milk treatment on the changes of the concetration of cholesterol and triglycerides in diabetetic white wistar rats. Conducted on May 15 until 15 August 2018. Experimental research pre-post test with control design. Subjects of twenty wistar white rats were made diabetic by injecting alloxan 140mg / kg. After being declared diabetic, the rats were divided into four groups, namely group 1 (negative control), group 2 (positive control), group 3 (bran milk 0.54g / 200 gr) and group 4 (bran milk 1.08g / 200gr). The intervention was given for fourteen days. The data analysis used the paired T-test to test the subject before and afterthe treatment and the One-Way ANOVA test was used to test the difference between the groups. The research results indicated that  the treatment with bran milk there were no significant differences in cholesterol and triglyceride concentrations in the four groups. But when viewed from changes before and after the intervention showed that group 4 (bran milk 1.08g / 200gr) decreased cholesterol concentration and in group 3 (bran milk 0.54g / 200gr) a decrease in triglycerides was better than group 1 (control negative) and group 2 (positive control). It was concluded that the administration of bran milk can reduce the concentration of cholesterol and triglycerides in wistar diabetic white rats.Keywords : Bran milk, Cholesterol, Triglycerides, Rats, Diabetic

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

scholarly journals Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae

2021 ◽  
Vol 9 (A) ◽  
pp. 760-765
Author(s):  
Yos Banne ◽  
Olfie Sahelangi ◽  
Steven Soenjono ◽  
Elisabeth Natalia Barung ◽  
Selfie Ulaen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Distilled Water ◽  
Lc50 Value ◽  
Acalypha Indica

BACKGROUND: Acalypha indica Linn. has been used as traditional medicine, it contains flavonoids, alkaloids, tannins, saponins, steroids, triterpenoids, and essential oils. AIM: This study aimed to determine the bio-larvicide effects of A. indica Linn. leaf stew and the silver nanoparticles against Anopheles sp. larvae. METHODS: The fresh leaves of A. indica Linn. extracted using distilled water at 100°C for 30 min. The silver nanoparticles were made by mixing a solution of silver nitrate with the stew, which acts as a reducing agent. The resulting silver nanoparticles were characterized by particle size analyzer and UV-vis spectrophotometer. The bio-larvicide effects against Anopheles sp. larvae performed using a completely randomized design. There were eight groups consisted of ten larvae and three replications. Treatment groups of stew and silver nanoparticle for concentrations 0.05%, 0.5%, and 5%, respectively. The negative control group was distilled water and the positive control group was the 0.01% abate solution. Assessment of larvicide activity was carried out every hour for 6 h and continued if there were larvae that live up to 24 h. The LC50 value was calculated based on Probit analysis. RESULTS: The results showed that the A. indica Linn. leaf stew can be made into silver nanoparticles preparations, optimal results were obtained from a mixture of 1% stew and 3 mM AgNO3. The result of bio-larvicides effect test against Anopheles sp. larvae showed that the LC50 value of the A. indica Linn. leaf stew was 727,3 ppm and the LC50 value of silver nanoparticles was 3.366 ppm. CONCLUSION: It can be concluded that A. indica Linn. is a promising larvicidal plant and can be made into silver nanoparticle preparations.

scholarly journals The Anti-tubercular Activity of Noni Fruitsto Inhibition Growth of Multi Drug Resistant-Tuberculosis Bacteria

2021 ◽  
pp. 979-990
Author(s):  
Novie Elvinawaty Mauliku
Keyword(s):  
Antitubercular Activity ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Resistant Tuberculosis ◽  
Crude Extracts ◽  
Group I ◽  
Mdr Tb

Multidrug-resistant tuberculosis (MDR-TB) is a tuberculosis infection that is resistant to the treatment at least two of the most powerful anti-tuberculosis drugs, such as Isoniazid and Rifampisin. Increased cases of MDR-TB in morbidity and mortality become obstacles in the control of tuberculosis (TB), thus requiring supportive treatment of natural ingredients that can contribute in the treatment of TB, such a noni fruit. The main objective of this study was extract of noni fruits to inhibition the growth of strain MDR-TB bacteria, and compered it with the anti-TB drugs. The Morinda c. Linn (Noni) fruits was extraced by ethanol (96%). The extract was filtered through whatman No.1 filter paper, evaporated to dryness on a water bath until the solvent evaporated completely and yield of the crude extract. The experiment were divided into 3 groups, i.e.: negative control: group I; positive control: group II; crude extracts noni fruit: group III: combinations of crude extracts noni fruit and anti-TB drugs (K, AK, and OF). Each group was divided into three groups’ doses of 30 mg/ml, 40 mg/ml and 50 mg/ml. The Anti-tuberculosis activities of extracts noni fruit and K, AK, and OF against TB-MDR bacteria were tested by susceptibility test using proportion method in Lowenstein-Jensen (LJ) media.The anti-tubercular activity of noni fruits was determined by the minimum inhibitory concentration (MIC) of the bacterial growth at various doses 30 mg/ml, 40 mg/ml, and 50 mg/ml. The research design used post-test only contol group, and analyzed using analysis of variance and post hoct test. The extracted of Morinda c.Linn (noni) fruits have antitubercular activity to inhibiton of growth MDR-TB bacteria at various doses (p value=0,000). At a dose 30 mg/mlthe mean rate of the growth colonies of MDR TB-bacteria whit the mean rate 59,00 ± 27,81, and at a dose 40 mg/ml was1,50 ± 2,81. While at a dose of 50 mg/ml the bacterial colonies of MDR-TB did not grow in media. The combination of Morinda c.Linn (noni) fruit with anti-tuberculosis drugs, was the smallest groups to inhibit and eliminate MDR-TB bacteria at a dose 30 mg/ml(0,00 ± 00.00). The experimental results confirmed the extracted of Morinda c.Linn (noni) fruits have antitubercular activity as well as anti-TB drugs, and the combination of the extracted of Morinda c.Linn (noni) fruits and anti-TB drugs was the best groups to inhibiton of growth MDR-TB bacteria.   Keywords: TB-MDR Bacteria, Morinda c. Linn (Noni), Anti-TB drugs, Anti-tubercular activity, MIC.

Comparison of conservative management and juvenile pubic symphysiodesis in the early treatment of canine hip dysplasia

2008 ◽  
Vol 21 (03) ◽  
pp. 267-279 ◽  
Author(s):  
G. Dravelli ◽  
L. Vezzoni ◽  
M. De Lorenzi ◽  
A. Corbari ◽  
A. Cirla ◽  
...  
Keyword(s):  
Early Treatment ◽  
Hip Dysplasia ◽  
Skeletal Maturity ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Homogeneous Groups ◽  
Group 2 ◽  
Canine Hip Dysplasia ◽  
Group 1

Summary Objectives: To evaluate the efficacy of juvenile pubic symphysiodesis (JPS) in a clinical setting for the early treatment of canine hip dysplasia (CHD), and to identify its indications and contraindications. Methods: The final degree of CHD using the FCI (Fédération Cynologique Internationale) CHD classification in 5 Grades (A, B, C, D, E) was assessed at skeletal maturity in two homogeneous groups of dogs assessed at the age of 14 to 22 weeks and selected according to their susceptibility to CHD; one group was treated with JPS and one group was conservatively managed. Two hundred seventeen puppies completed the study; 81 were treated with JPS (group 1) and 76 were conservatively managed (group 2). A third group of 60 puppies with normal hips was followed as a negative control group. Results: In group 1, 43.2% of the puppies had regression or a lack of progression of the disease in the final evaluation (Grade A & B), 25.9% had mild CHD (Grade C) and 30.9% had moderate and severe CHD (Grade D & E). In group 2, 23.6% of the puppies did not show any development of the disease (Grade A & B), 21.1% had mild CHD (Grade C) and 55.3% developed moderate to severe CHD (Grade D & E). Further investigation was done by comparing the severity of early signs of susceptibility to CHD with the final FCI Grades at adulthood in both groups. Clinical significance: The JPS procedure increased the odds of arresting or limiting the progression of CHD in mild to moderate grades of CHD, while it was less effective or ineffective in more severe forms.

scholarly journals Comparative Study of the Bioactivity of Two Bioceramic Materials

2018 ◽  
Vol 21 (2) ◽  
pp. 73-81
Author(s):  
Margarita Trujillo-Hernández ◽  
Rebeca E. Flores-Ventura ◽  
Angélica Suárez-Porras MSc ◽  
Leandro García-González PhD ◽  
Julián Hernández-Torres PhD ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Calcium Phosphate ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Carbonated Apatite ◽  
Human Dentine ◽  
Group 2 ◽  
Group 1

Calcium silicate-based materials have been shown to be bioactive due to their ability to produce biologically compatible carbonated apatite. The objective of this study was to analyze the bioactivity of Biodentine ™ and MTA Repair HP® in contact with human dentine discs, which were sealed and divided randomly to form four groups: group 1 Biodentine™, group 2 MTA Repair HP®, positive control group MTA Angelus® and negative control group IRM®, which were incubated in PBS solution for 10 days, for a subsequent analysis by means of MEB-EDS and Raman spectroscopy. The three calcium-based materials analyzed in this study proved to be bioactive because upon contact with a phosphate-based solution they were triggered at the onset of amorphous calcium phosphate, as the precursor during the formation of carbonated apatite.

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