scholarly journals PENGARUH PEMBERIAN JUS BUAH PEPAYA (Carica Papaya L) TERHADAP GAMBARAN HISTOPATOLOGIK FATTY STREAK PADA DINDING AORTA ABDOMINALIS TIKUS WISTAR JANTAN HIPERKOLESTEROLEMIK

2014 ◽  
Vol 6 (3) ◽  
Author(s):  
Hendra Sutysna ◽  
Iskandar Japardi ◽  
Soekimin .
Keyword(s):  
Wistar Rats ◽  
Carica Papaya ◽  
Aortic Wall ◽  
Foam Cells ◽  
Control Group ◽  
Fatty Streak ◽  
Histopathological Changes ◽  
Abdominal Aortic ◽  
Post Hoc ◽  
Aorta Abdominalis

Abstract: Papaya (Carica papaya L) is rich of antioxidants, such as betacarotene, which is a powerful antidote to reactive oxygen compound (ROS) and stimulates the body to convert toxic substances into harmless compounds. In atherosclerotic lesions, antioxidants inhibit the oxidation of LDL cholesterol and prevent the oxidative stress, therefore, reducing the vascular endothelial dysfunction. This study aimed to obtain the effect of papaya given orally on histopathological changes which was determined by numbers of foam cells and abdominal aortic wall thickness of male hypercholesterolemic wistar rats. This was an experimental study. Samples were 24 adult male wistar rats divided into five groups. The negative control group K1 was fed on standard diet for 2 weeks and the positive control group K2, each was fed on diet of egg yolks 5 mg/day for 2 weeks. There were three treatment groups: P1, each was fed on papaya juice 2,6 g/day for 2 weeks; P2, each was fed on papaya juice 2,6 g/day for 4 weeks; and P3, each was fed on papaya juice 2,6 g/day for 6 weeks by using nasogastric tube. After the treatment period of each sample group, the wistar rats were decapitated, and then the levels of total cholesterol and LDL of blood serum were examined as well as the histopathological changes were identified. Data were analyzed by using Oneway-Anova test, followed by Post-Hoc test. The results showed that among P1, P2, and P3 groups there were reductions in the numbers of foam cells in the walls of the abdominal aorta and in the thickness of abdominal aortic wall compared to the K2. Conclusion: Papaya juice which was given orally for 2-4 weeks could reduce the histopathological changes of abdominal aortic walls of male hypercholesteremic wistar rats.Keywords: papaya, antioxidant, hypercholesterolemic, histopathological changes, fatty streakAbstrak: Pepaya (Carica papaya L) kaya akan antioksidan beta-karoten yang merupakan penawar kuat untuk senyawa oksigen reaktif (ROS) dan berkemampuan menstimulasi tubuh untuk mengubah substansi toksik menjadi senyawa yang tidak berbahaya. Pada lesi aterosklerotik, antioksidan dapat menghambat oksidasi kolesterol LDL dan stres oksidatif sehingga mengurangi terjadinya disfungsi endotel pembuluh darah. Penelitian ini bertujuan untuk mengetahui efek pemberian jus buah pepaya secara oral terhadap gambaran histopatologik yaitu jumlah sel busa dan ketebalan dinding pembuluh darah dari aorta abdo-minalis tikus wistar jantan yang hiperkolesterolemik. Penelitian ini bersifat eksperimental dengan rancangan Randomized Post-test Only Control Group Design. Sampel 24 ekor tikus wistar jantan dewasa dibagi dalam 5 kelompok. Kelompok kontrol negatif K1 diberi diet pakan standar selama 2 minggu dan kelompok kontrol positif K2 diberi diet kuning telur 5 mg/ekor/hari selama 2 minggu. Terdapat tiga kelompok perlakuan: P1 diberi jus pepaya 2,6 g/ekor/hari selama 2 minggu; P2 diberi jus pepaya 2,6 g/ekor/hari selama 4 minggu; dan P3 yang diberi jus pepaya 2,6 g/ekor/hari selama 6 minggu melalui sonde hidung. Setelah masa perlakuan masing-masing kelompok, sampel didekapitasi, kemudian diukur kadar kolesterol total dan LDL serum darah, serta diidentifikasi gambaran histopatologik yaitu jumlah sel busa dan ketebalan dinding aorta abdominalis. Data dianalisis dengan uji Oneway- Anova dan dilanjutkan dengan uji Post-Hoc. Hasil penelitian menunjukkan bahwa pemberian jus buah pepaya 2,6 gram/ekor pada kelompok perlakuan P1, P2, dan P3 dapat menurunkan jumlah sel busa pada dinding aorta abdominalis dan ketebalan dinding aorta abdominalis tikus wistar jantan yang hiperkolesterolemik dibandingkan dengan kelompok kontrol positif K2. Simpulan: Pemberian jus pepaya per oral selama 2-4 minggu dapat menurunkan perubahan histopatologik dinding aorta abdominalis tikus wistar jantan yang hiperkolesterolemik.Kata kunci: pepaya, antioksidan, hiperkolesterolemik, histopatologik, fatty streak.

scholarly journals GAMBARAN HISTOLOGIK AORTA TIKUS WISTAR DENGAN DIET LEMAK BABI SETELAH PEMBERIAN EKSTRAK DAUN PEPAYA

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Friska W. F. Panjaitan ◽  
Marie M. Kaseke ◽  
George N. Tanudjaja
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Group Iv ◽  
Control Group ◽  
Histological Features ◽  
Group I ◽  
Group Ii ◽  
Lard Diet ◽  
Control Group Group

Abstract: Papaya leaves contain several antioxidants (flavonoid, tanin, and vitamin C) which have antiatherogenic effects that may inhibit the progression of an atherosclerotic lesion. One of the risk factors causing atherosclerosis is the consumption of food containing highly saturated fatty acids e.g. lard. Low density lipoproteins (LDL) accumulate within the intima and then are oxidized (LDL-ox). This LDL-ox is ingested by macrophages, resulting in foam-cell formation (early lesion of atherosclerosis). This study aimed to find out the histological features of the aorta of wistar rats having lard diets without the addition of the papaya leaf extract; having lard diets along with the papaya leaf extract; and having lard diets followed by papaya leaf extract. This was an experimental study on 16 wistar rats divided into 4 groups: group I without treatment (negative control group), group II lard diet for 14 days (positive control group), group III lard diet with papaya leaf extract for 14 days (treatment group I), and group IV lard diet for 14 days, and then followed by papaya leaf extract for 14 days (treatment group II). It was found that the aorta of group I showed adipose cells in the intima and media layers; group II and III showed foam cells in both layers; and group IV showed foam cells in fewer numbers than group II. Conclusion: The aorta histological features of wistar rats given lard diets for 14 days, with or without papaya leaf extract, showed foam cells in the intima and media layers. Papaya leaf extraxt added to lard diets had no effect on decreasing foam cells (no protective effect), meanwhile papaya leaf extract following lard diets showed a reduction of foam cells (therapeutic effect). Keywords: papaya leaf, lard dietary, foam cells, wistar rat.   Abstrak: Daun pepaya mengandung antioksidan (flavonoid, vitamin C) yang berefek anti-aterogenik, sehingga diharapkan dapat menghambat perkembangan lesi aterosklerosis. Salah satu faktor risiko penyebab aterosklerosis yaitu makanan yang berkandungan tinggi asam lemak jenuh, antara lain lemak babi. Konsumsi lemak jenuh berlebihan dapat mengganggu fungsi sel endotel, sehingga lipoprotein berdensitas rendah (LDL) dapat masuk dan menjadi LDL teroksidasi (LDL-oks). Makrofag menangkap LDL-oks dan menjadi sel busa (lesi dini aterosklerosis). Penelitian ini bertujuan untuk mengetahui gambaran histologik aorta tikus wistar dengan diet lemak babi tanpa pemberian ekstrak daun pepaya, diet lemak babi bersamaan pemberian ekstrak daun pepaya, dan setelah diet lemak babi dilanjutkan pemberian ekstrak daun pepaya. Penelitian ini bersifat eksperimental. Subyek penelitian terdiri dari 16 ekor tikus wistar yang dibagi menjadi empat kelompok: kelompok I tanpa perlakuan (kelompok kontrol negatif); kelompok II dengan diet lemak babi selama 14 hari (kelompok kontrol positif); kelompok III dengan diet lemak babi serta pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan I); dan kelompok IV dengan diet lemak babi selama 14 hari, dilanjutkan pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan II). Hasil penelitian memperlihatkan gambaran histologi aorta kelompok I tampak perlemakan; pada kelompok II terdapat sel-sel busa; pada kelompok III masih terdapat sel-sel busa; dan pada kelompok IV terdapat sel-sel busa, namun dalam jumlah yang lebih sedikit dibandingkan pada gambaran kelompok II. Simpulan: Tikus wistar dengan diet lemak babi selama 14 hari, baik dengan maupun tanpa ekstrak daun pepaya, memperlihatkan gambaran histologik adanya sel-sel busa pada tunika intima dan tunika media aorta. Pemberian ekstrak daun pepaya bersamaan dengan diet lemak babi tidak berefek menurunkan jumlah sel busa (tidak ada efek protektif) sedangkan pemberian ekstrak daun pepaya setelah diet lemak babi berefek mengurangi jumlah sel-sel busa yang terbentuk (efek terapi). Kata kunci: daun pepaya, diet lemak babi, sel-sel busa, tikus wistar.

scholarly journals THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

2021 ◽  
Vol 6 (2) ◽  
pp. 159
Author(s):  
Nabilah Nabilah ◽  
Irham Taufiqurrahman ◽  
Nolista Indah Rasyid
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Gel Effect ◽  
Index Measurement ◽  
Male Wistar Rats ◽  
Collagen Density ◽  
Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

scholarly journals Pengaruh cabe rawit terhadap gambaran histopatologik lambung tikus Wistar yang diinduksi aspirin

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Abel T. Bawulele ◽  
Lily Loho ◽  
Poppy Lintong
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Control Group ◽  
Histopathological Changes ◽  
Acute Gastritis ◽  
Group B ◽  
Group D ◽  
Mucosa Epithelium

Abstract: Cayenne pepper (Capsicum frustescens L ) is commonly in daily food. This study aimed to determine the effect of cayenne pepper to histopathological changes in gaster of Wistar rats induced by aspirin. This was an experimental study. Subjects were Wistar rats consisted of 5 rats as the negative control and 20 rats as the treatment group. Group A, the negative control group, was untreated; group B was treated with aspirin 21mg/day for 10 days; group C was treated with aspirin 21mg/day for 10 days together with cayenne pepper 84mg once daily; group D was treated with aspirin 21mg/day for 10 days followed by cayenne 84mg twice daily; and group E was treated with aspirin 21mg/day for 10 days and then was untreated for 3 days. The histopathological changes in group C showed a lot of inflammatory cells and erosion of gastric mucosa epithelium compared to group B, D, and E. Group D also showed inflammatory cells but no erosion as in group C. Group E had less inflammatory cells than group B, C, and D. Conclusion: Administration of aspirin 21mg/kg BW for 10 days led to acute gastritis in Wistar rat. Administration of aspirin together with cayenne 84mg/day led to acute gastritis and erosion of gastric mucosa epithelium. However, administration of cayenne pepper 168mg/day after aspirin led to acute gastritis without erosionKeywords: pepper, gaster, aspirinAbstrak : Cabe Rawit (Capsicum frustescens L.) banyak dijumpai dalam makanan sehari-hari. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian cabe rawit terhadap gambaran histopatologik lambung tikus wistar yang diinduksi aspirin. Jenis penelitian ialah eksprimental. Subjek penelitian ialah tikus wistar, yang dibagi atas: 5 ekor untuk kontrol negatif dan 20 ekor untuk perlakuan. Kelompok A (kontrol negatif) tidak diberi perlakuan; kelompok B diberikan aspirin 21mg/hari selama 10 hari; kelompok C diberikan aspirin 21mg/hari selama 10 hari bersama cabe rawit 84mg sekali sehari; kelompok D diberikan aspirin 21mg/hari selama 10 hari kemudian dilanjutkan pemberian cabe rawit 84mg selama 3 hari 2 kali sehari; dan kelompok E diberikan aspirin 21mg/hari selama 10 hari kemudian tidak diberi perlakuan selama 3 hari. Hasil penelitian menunjukkan bahwa pada kelompok C terlihat banyak sel-sel radang disertai erosi epitel mukosa lambung dibanding dengan kelompok B, D, dan E. Pada kelompok D pemberian aspirin dilanjutkan dengan cabe rawit dosis 2 kali sehari masih terdapat sel-sel radang tetapi tidak terjadi erosi seperti pada kelompok C. Kelompok E memiliki sel-sel radang yang lebih sedikit dibandingkan kelompok B, C, dan D. Simpulan: Pemberian aspirin 21mg/hari selama 10 hari menyebabkan gastritis akut pada tikus wistar. Pemberian aspirin bersama cabe rawit 84mg/hari menyebabkan gastritis akut erosi sedangkan pemberian cabe rawit 168mg/hari setelah diberi aspirin menyebabkan gastritis akut tanpa erosi.Kata kunci: cabe rawit, lambung, aspirin

scholarly journals Pengaruh pemberian ekstrak tinta cumi (squid ink) terhadap aterosklerosis

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Fajar M. Nasution ◽  
Rina S. Mardia ◽  
Ayu Azri ◽  
Rido R. Hutabarat ◽  
Fadhila Al. Izza ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Wistar Rats ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Foam Cells ◽  
Control Group ◽  
Test Design ◽  
Randomized Controlled ◽  
Post Test ◽  
Paired T Test

Abstract: Squid ink consists of DHI, DHICA, and 2-carboxyl indole that improve lipid profile and remove foam cells in blood vessel walls. This study was aimed to analyze the effect of squid ink on atherosclerosis-induced Wistar rats. This was an experimental study. We used randomized controlled trial with pre-post test design for lipid profile assessment and randomized post-test only control design for blood vessel evaluation (foam cells in layers of coronary artery). Samples were 27 Wistar rats, divided into three groups; all were atherosclerosis-induced with initial injection of 0.006mg iv adrenalin on day 1, followed by standard diet and egg yolk until day 30. Squid ink extract was given to group P1 and P2 on days 15-30 in different doses, but not to control group. Data were analyzed by using Saphiro-Wilk test and paired t-test. The results showed that P2 group had decreased means of total cholesterol (-33.62 mg/dl), triglyceride (-28.00 mg/dl), and LDL cholesterol (-28.16 mg/dl), but an increased mean of HDL cholesterol (11.60 mg/dl). There were decreased numbers of foam cells in the layers of coronary arteries of P1 and P2 group compared to the control group. Conclusion: Squid ink extract could lower total cholesterol, triglyceride, and LDL cholesterol levels, and increase HDL cholesterol level. Moreover, it could remove foam cells from the layers of coronary arteries.Keywords: squid ink, atherosclerosis, lipid profile, foam cells Asbtrak: Tinta cumi memiliki kandungan DHI, DHICA, dan 2-carboxyl indole yang dapat memperbaiki profil lipid dan mengurangi sel busa pembuluh darah. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak tinta cumi terhadap tikus yang diinduksi aterosklerosis. Jenis penelitian ialah eksperimental dengan randomized controlled with pre-post test design untuk penilaian profil lipid dan post test only design untuk penilaian pembuluh darah (sel busa dalam dinding arteri koronaria). Sampel terdiri dari 27 tikus Wistar jantan, dibagi secara random menjadi tiga kelompok; kesemuanya diberi induksi aterosklerosis dengan injeksi inisial adrenalin 0,006 mg iv pada hari ke-1, dilanjutkan diet standar dan diet kuning telur sampai hari ke-30. Tikus kelompok kontrol tidak diberikan ekstrak tinta cumi; tikus kelompok perlakuan 1 dan perlakuan 2 diberikan tambahan ekstrak tinta cumi pada hari ke 16 sampai hari ke 30 dengan dosis yang berbeda. Analisis data dengan uji Saphiro-Wilk dan uji paired t-test. Hasil penelitian memperlihatkan penurunan rerata setelah perlakuan pada kelompok P2 ialah kolesterol total (-33,62 mg/dl), trigliserida (-28,00 mg/dl), dan kolesterol LDL (-28,16 mg/dl), serta peningkatan rerata kolesterol HDL (11,60 mg/dl). Terdapat penurunan jumlah sel busa kelompok perlakuan P1 dan P2 dibandingkan kelompok kontrol. Simpulan: Pemberian ekstrak tinta cumi menurunkan kadar kolesterol total, trigliserida dan kolesterol LDL, meningkatkan kadar kolesterol HDL, serta menurunkan jumlah sel busa dalam dinding arteri koronaria.Kata kunci: tinta cumi, aterosklerosis, profil lipid, sel busa

scholarly journals The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays

Sains Medika ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 20
Author(s):  
Anwar Riyanto ◽  
Taufiqurrachman Nasihun ◽  
Titiek Sumarawati
Keyword(s):  
Green Tea ◽  
Wistar Rats ◽  
Control Group ◽  
Topical Cream ◽  
Male Wistar Rats ◽  
The Difference ◽  
Tyrosinase Enzyme ◽  
Uvb Exposure ◽  
Post Hoc ◽  
Better Than

Introduction: Green tea and tocopherol are potent antioxidants used to treat melasma. However, whether green tea or tocopherol is superior remains unclear. Objective: To compare the effectiveness between green tea and tocopherol in decreasing the amount of melanin and tyrosinase level in wistar rats exposed to UVB rays.Methods: The experimental study took 30 male Wistar rats randomly and divided them into 3 groups. The control group (C-G) was given basic topical cream, GT-G was given green tea topical cream and TC-G was given tocopherol topical cream. All rats were exposed to UVB every Monday, Wednesday and Friday for 4 weeks, whereas topical creams were smeared every day. Topical creams smearing on the same day with UVB exposure was performed 20 minutes before exposure and 4 hours after UVB exposure. The doses of UVB were 50 mJ/cm2 in first week, 70mJ/cm2 in the second week and 80mJ/cm2 in the third and fourth weeks. The amount of melanin was measured using pixel method and the tyrosinase level was measured using ELISA.Results: Anova analysis indicates that the amount of melanin and tyrosinase level are significantly different between groups, p<0.05. Post Hoc LSD analysis indicates that the amount of melanin in GT-G and TC-G are significantly lower than that of C-G, p<0.05. The amount of melanin in GT-G is lower than that of TC-G, p<0.05. The tyrosinase level in GT-G is significantly lower than that of C-G and TC-G, p<0.05. Meanwhile, the tyrosinase level in TC-G is lower than that of C-G but insignificantly, p>0.05.� Conclusion: Green tea topical treatment is significantly capable of decreasing the amount of melanin and tyrosinase level better than tocopherol.

scholarly journals GAMBARAN HISTOPATOLOGI HATI TIKUS WISTAR YANG DIBERIKAN JUS TOMAT (Solanum Lycopersicum) PASCA KERUSAKAN HATI WISTAR YANG DIINDUKSI KARBON TETRAKLORIDA (CCl4)

2013 ◽  
Vol 1 (3) ◽  
Author(s):  
Eka Sari Tappi ◽  
Poppy Lintong ◽  
Lily Loho
Keyword(s):  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Liver Cells ◽  
The Body ◽  
Tomato Juice ◽  
Control Group ◽  
Histopathological Changes ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Abstract: Liver is the largest organ in the abdominal cavity. As the center of metabolism in the body, liver is potentially damaged by exposure of toxic substances, inter alia carbon tetrachloride (CCl4). Metabolism of carbon tetrachloride (CCl4) produces CCl3 free radicals that can damage the liver. In Indonesia, there are a lot of natural ingredients that have antioxidant properties, such as tomato. Lycopene in tomatoes contains antioxidant compounds that can prevent damages due to free radical. This study aimed to obtain liver histopathological changes of wistar rats fed with tomato juice after being induced of carbon tetrachloride (CCl4). This was an experimental study, using 10 wistar rats which were divided into 4 groups. Group I was the negative control; group II was induced with CCl4 0,05 cc/day and was terminated on day 6; group III was induced with CCl4 0,05 cc/day and was given tomato juice 3 ml/day, and terminated on day 13; group IV was induced by CCl4 0,05 cc/day, given regular pellets, and terminated on day 13. The results showed that group II had histopathological changes of the liver indicating fatty liver, meanwhile group III showed regeneration of nearly all liver cells. Conclusion: Administration of tomato juice after the induction of 3 ml carbon tetrachloride (CCl4) for 7 day showed regeneration of almost all liver cells. Keywords: histopathological changes of the liver, carbon tetrachloride, tomato juice.   Abstrak: Hati merupakan organ terbesar dalam rongga abdomen, dan pusat metabolisme tubuh dengan fungsi yang sangat kompleks dan sangat berpotensi mengalami kerusakan akibat terpapar oleh bahan-bahan toksik, salah satunya yaitu karbon tertraklorida (CCL4). Metabolisme CCl4 menghasilkan radikal bebas CCl3 yang dapat merusak hati. Di Indonesia terdapat  banyak sekali bahan-bahan alami yang mempunyai kandungan antioksidan, salah satunya yaitu tomat. Tomat mengandung senyawa likopen sebagai antioksidan yang dapat mencegah kerusakan jaringan akibat radikal bebas. Penelitian ini bertujuan untuk mendapatkan gambaran histopatologi hati tikus wistar yang diberi jus tomat pasca induksi karbon tetraklorida (CCl4). Metode penelitian ialah eksperimental. Sampel sebanyak 10 ekor tikus wistar yang dibagi dalam 4 kelompok. Kelompok I sebagai kontrol negatif; kelompok II diinduksi CCl4 0,05cc/perhari dan diterminasi hari ke-6; kelompok III diinduksi CCl4 0,05 cc/hari kemudian diberikan jus tomat 3ml/hari;  dan kelompok IV diinduksi CCl4 0,05 cc/hari kemudian diberikan pelet biasa dan diterminasi hari ke-13. Hasil penelitian menunjukkan pada pemberian CCl4 pada tikus wistar selama 5 hari terdapat gambaran morfologik perlemakan sel hati. Pemberian jus tomat dosis 3 ml pasca induksi karbon tetraklorida (CCl4) menunjukkan terjadinya regenerasi pada hampir seluruh sel-sel hati. Simpulan: Pemberian jus tomat dosis 3 ml pasca induksi karbon tetraklorida (CCl4) selama 7 hari menunjukkan regenerasi pada hampir seluruh sel-sel hati. Kata kunci: gambaran histopatologi hati, karbon tetraklorida, jus tomat.

scholarly journals PENGARUH EKSTRAK DAUN GEDI (Abelmoschus manihot L.) TERHADAP GAMBARAN HISTOPATOLOGI AORTA TIKUS WISTAR DENGAN DIET ATEROGENIK

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Nadya Nadiratika Papodi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Blood Cholesterol ◽  
Control Group ◽  
Pork Lard ◽  
Pork Fat

Abstract: High blood cholesterol is a major risk factor for atherosclerosis. Gedi plants contain polyphenolic compounds that can lowering blood cholesterol levels. This study aimed to find out the effect of gedi (Abelmoschus manihot L) leaves extract on histopatologic features of aorta of those wistar rats, which had been administered an atherogenic diet. This was an experimental study on animal using 20 Wistar rats as samples. The rats were divided into 4 different groups, 5 rats for each group. Negative control group was fed only pelet, positive control group was fed pork fat as much as 2 ml/day, group C was fed on pork fat and gedi leaf extract, and group D was fed on pork fat then continued with gedi leaf extract. The results indicated that administration of 30 mg gedi leaf extract showed a histopathologic feature which is decreasing of foam cells. Conclusion: Microscopically, aorta of those rats fed on pork lard diet showed a number of foam cells in tunica intima and media whereas the rats that were fed on pork lard along with gedi leaf extract, generally there were no foam cells, but there aresome parts which contain few foam cells. On the group of Wistar rats which were fed on pork lard, continued with gedi leaf extract showed less foam cells compared to the positive control group.Keywords: Gedi leaf extract, pork lard, foam cell.Abstrak: Kadar kolesterol darah yang tinggi merupakan faktor risiko utama terjadinya aterosklerosis. Tumbuhan gedi mengandung senyawa polifenol yang dapat menurunkan kadar kolesterol darah. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun gedi terhadap gambaran histopatologi aorta tikus wistar dengan diet aterogenik. Hewan uji yang digunakan berupa 20 ekor tikus Wistar yang dibagi ke dalam 4 kelompok, masing-masing kelompok terdiri dari 5 ekor tikus. Kelompok kontrol negatif hanya diberikan pelet biasa, kelompok kontrol positif diberikan diet lemak babi 2ml/hari, kelompok C diberikan diet lemak babi bersamaan dengan ekstrak daun gedi, dan kelompok D diberikan lemak babi kemudian dilanjutkan dengan pemberian ekstrak daun gedi. Hasil penelitian memperlihatkan bahwa pemberian ekstrak daun gedi 30 mg mampu memberikan gambaran histopatologi aorta berupa berkurangnya jumlah sel busa. Simpulan: Secara mikroskopik, aorta tikus wistar yang diberi lemak babi menunjukkan adanya penumpukan sel-sel busa pada tunika intima dan media, sedangkan pada aorta tikus wistar yang diberi lemak babi bersamaan dengan ekstrak daun gedi maupun yang dilanjutkan dengan ekstrak daun gedi ditemukan berkurangnya jumlah sel-sel busa.Kata Kunci : Ekstrak daun gedi, Lemak babi, Sel busa.

scholarly journals Effect of ovarian autotransplantation on FSH levels in Wistar rats late menopause model

2018 ◽  
Vol 26 (1) ◽  
pp. 42
Author(s):  
Abdurahman Laqif ◽  
Dewi Kartika DJ Anwar ◽  
Eriana Melinawati
Keyword(s):  
Data Analysis ◽  
Wistar Rats ◽  
Control Group ◽  
Bilateral Oophorectomy ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
Post Hoc ◽  
Group 1 ◽  
Elisa Data

Objectives: To determine the effect of ovarian autotransplan-tation on decreasing FSH level in Wistarrats late menopause model.Materials and Methods: Experimental analytic research on 27 Wistar rats (Rattus novergicus) was divided into 3 groups. Group 1 (K1) or control. Group 2 (K2) performed bilateral oophorec-tomy without autotransplantation. Group 3 (K3) performed bilateral oophorectomy with autotransplantation. Measurements of FSH levels were performed on the first day, day 28 (four weeks after bilateral oophorectomy, during late menopause) and 56 day (four weeks after autotransplant). Measurement of FSH levels using ELISA. Data analysis used ANOVA and Post Hoc test.Results: The mean FSH level measured on day 56 at K1 = 63.400 ng/mL, at K2 = 78.416 ng/mL and K3 = 31.991 ng/mL. There were significant differences between K1 and K2 (p = 0,000), K1 and K3 (p = 0,000), and between K2 and K3 (p = 0,000).Conclusion: Ovarian autotransplantation decrease FSH levels in Wistar rats late menopause model.

scholarly journals The effect of platelet-rich fibrin on the acceleration of osteoblast proliferation after endosseous dental implant insertion

2020 ◽  
Vol 6 (1) ◽  
pp. 55
Author(s):  
Evan Chandra ◽  
Suparyono Saleh ◽  
Murti Indrastuti
Keyword(s):  
Bone Regeneration ◽  
Dental Implants ◽  
Dental Implant ◽  
Wistar Rats ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Platelet Rich Fibrin ◽  
Anova Test ◽  
Implant Bed ◽  
Post Hoc

The osseointegration process of dental implants begins with osteoblast differentiation and proliferation, which is an important aspect of the bone regeneration process. Platelet-rich fibrin (PRF) is a platelet that contains a large amount of fibrin and growth factor, which is widely used to accelerate bone regeneration. This study aims to determine the effect of PRF on the acceleration of osteoblast proliferation after endosseous dental implant insertion. Twelve male Wistar rats weighing 250 to 300 gr of 8 to 15 weeks as an animal model were divided into two groups, the control group and treatment group, based on days and PRF treatment. Three ml of Wistar rat blood was obtained and centrifuged for 12 minutes at a speed of 2700 rpm to make PRF. Afterward, platelet-rich fibrin was applied to implant bed, and the dental implant was inserted at the lateral epicondyle of the right femur in 3 mm depth and 1.8 mm diameter dimension. Incontrol groups, dental implants were immediately inserted after implant bed preparation without PRF administration. Observation of the Wistar rats was carried out on days 14 and 28 for each group. The rats were terminated accordingto the timeline of group design. The epicondylus lateralis femoris dextra bone of the rats was taken and fixed with 10% buffered formalin solution. Then, histological samples were made with hematoxylin-eosin staining. Sample observation was done under a light microscope to calculate the number of osteoblasts. The data were analyzed using the two-way ANOVA test, followed by LSD post hoc test. Based on the two-way ANOVA test, there was a significant difference between control groups and treatment groups (p<0.05), and there were significant differences between the observation result of days 14 and 28 (p<0.05). The application of PRF increased osteoblast proliferation for the first 14 days, but the rate decreased after that. Based on the LSD post hoc test, there were differences in osteoblast proliferation between the treatment and control group (p<0.05). Thus, it is concluded that platelet-rich fibrin increased the acceleration ofosteoblast proliferation after endosseous dental implant insertion.

scholarly journals GAMBARAN HISTOPATOLOGI AORTA TIKUS WISTAR YANG TERPAPAR ASAP ROKOK

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Lapatta Nazlyza
Keyword(s):  
Blood Vessels ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Foam Cell ◽  
Foam Cells ◽  
Negative Control ◽  
Fatty Streak ◽  
Group A ◽  
Group B ◽  
Tunica Intima

Abstract: Cigarette smoking is one of the high risk factor of atherosclerosis. Atherosclerosis signed by plaque in artery and can caused constriction in the blood vessels. Atherosclerosis often occured in the blood vessels of the aortic. The purpose of these study is to see the histopatologic image of wistar rats aortic after being exposed by cigarrete smoke.          This experimental study conducted throughout 5months using 10 rats wistar devided into 3 groups. Group A as a negative control (2 rats). Group B are exposed by cigarette smoke as much 24 cigarettes for 20 days (4 rats). Group C exposed by cigarette smoke as much 20 cigarette per day for 30 days (4 rats). The rats has been autopsied at the last 20 and 30 days and then continue made histology preparation with HE staining. The examination result shows by the microscopic image of the group A wistar rats aortic are normal. Group B showed a layer of foam cells in the tunica intima media. Group C showed foam cells in the intima and the media have started to protrude into the lumen. As a conclusion the wistar rats that are exposed by cigarrete smoke during 20 to 30 days showed a fatty streak (foam cell) on tunica intima and media aortic as early lesions in the process of atherosclerosis. Keywords: atherosclerosis, cigarette.     Abstrak: Rokok merupakan salah satu faktor pencetus terjadinya aterosklerosis. Aterosklerosis ditandai oleh adanya plak di arteri yang dapat menyebabkan penyempitan pada pembuluh darah. Aterosklerosis sering terjadi pada pembuluh darah aorta. Tujuan penelitian ini untuk dapat melihat gambaran histopatologi aorta tikus wistar setelah dipapar dengan asap rokok. Penelitian eksperimental ini dilakukan selama 5 bulan dengan menggunakan 10 ekor tikus wistar yang dibagi dalam 3 kelompok. Kelompok A kontrol negatif (2 ekor). Kelompok B dipapari asap rokok sebanyak 24 batang perhari selama 20 hari (4 ekor). Kelompok C dipapari asap rokok sebanyak 24 batang perhari selama 30 hari (4ekor). Tikus diotopsi pada hari ke 20 dan 30 dan dibuat preparat histologi dengan pengecatan HE. Hasil pemeriksaan menunjukkan gambaran mikroskopik aorta tikus wistar kelompok A normal. Kelompok B menunjukkan adanya sel busa pada lapisan tunika intima, sampai tunika media. Kelompok C menunjukkan sel busa pada tunika intima sampai media dan sudah mulai menonjol ke lumen. Simpulan: tikus wistar yang dipapari asap rokok selama 20 sampai 30 hari menunjukkan adanya fatty streak (selbusa) pada tunika intima dan media aorta sebagai lesi awal dalam proses aterosklerosis. Kata Kunci: aterosklerosis, rokok.

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