scholarly journals UJI AKTIVITAS ANTIBAKTERI SEDIAAN SABUN CAIR EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 767
Author(s):  
Evander Roliand Lomboan ◽  
Paulina V.Y. Yamlean ◽  
Elly J. Suoth
Keyword(s):  
Staphylococcus Aureus ◽  
Specific Gravity ◽  
Moisture Content ◽  
Foam Height ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Syzygium Aromaticum ◽  
Liquid Soap

ABSTRACT Clove leaf (Syzygium aromaticum) is a plant that contains eugenol, saponins, flavonoids and tannins which have antibacterial properties. This study aims to formulate the ethanol extract liquid soap of clove leaves and test the effectiveness of the preparation against Staphylococcus aureus bacteria. This research was conducted in a laboratory experiment to make liquid soap with extract concentrations of 4%, 7%, 9%, 12% and 15%, then the soap was tested for spesifications including organoleptic testing, pH, foam height, moisture content, free alkaline content, and specific gravity. Antibacterial effectiveness testing against the growth of Staphylococcus aureus was carried out using the diffusion method. Clove leaf ethanol extract (Syzygium aromaticum) can be formulated into liquid soap. The results of testing the quality of liquid soap preparations meet the requirements in accordance with SNI. The results obtained from the antibacterial effectiveness test of liquid soap ethanol extract of Clove leaves can inhibit Staphylococcus aureus bacteria with all concentrations in the strong category, with a concentration of 15% having the largest diameter of 17,33 mm. Keywords : Antibacterial, Liquid Soap, Syzygium aromaticum, Staphylococcus aureus ABSTRAK Daun Cengkeh (Syzygium aromaticum) merupakan tanaman yang memiliki kandungan eugenol, saponin, flavonoid dan tannin yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasi sabun cair ekstrak etanol daun Cengkeh dan pengujian efektivitas sediaan terhadap bakteri Stahpylococcus aureus. Penelitian ini dilakukan secara eksperimental laboratorium untuk membuat sabun cair dengan konsentrasi ekstrak yaitu 4%, 7%, 9%, 12%, dan 15%, selanjutnya sabun diuji spesifikasinya meliputi pengujian organoleptik, pH, tinggi busa, kadar air, kadar alkali bebas, dan bobot jenis. Pengujian efektivitas antibakteri terhadap pertumbuhan Staphylococcus aureus dilakukan dengan menggunakan metode difusi. Ekstrak etanol daun Cengkeh (Syzygium aromaticum) dapat diformulasikan menjadi sabun cair. Hasil pengujian mutu sediaan sabun cair memenuhi persyaratan sesuai dengan SNI. Hasil uji efektivitas antibakteri sabun cair ekstrak etanol daun Cengkeh yang diperoleh dapat menghambat bakteri Staphylococcus aureus dengan semua konsentrasinya termasuk dalam kategori kuat, dengan konsentrasi 15% yang memiliki diameter paling besar yaitu 17,33 mm. Kata Kunci: Antibakteri, Sabun Cair, Syzygium aromaticum , Staphylococcus aureus

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI SABUN CAIR KOMBINASI EKSTRAK ETANOL DAUN JATI (Tectona grandis Linn.f.) DAN DAUN EKOR KUCING (Acalypha hispida burm.f.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 558
Author(s):  
Nia Kartika Pareda ◽  
Hosea J. Edy ◽  
Julianri S. Lebang
Keyword(s):  
Staphylococcus Aureus ◽  
Specific Gravity ◽  
Moisture Content ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Tectona Grandis ◽  
Liquid Soap ◽  
Extract Concentration

ABSTRACTTeak leaves (Tectona grandisLinn. f.) and Ekor Kucing leaves (Acalypha hispida Brum. f.) are plants that contain secondary metabolite compounds that can inhibit  bacteria.  This  research  aimsisto  formulate  liquid  soap containethanol extract of Teak leaves (Tectona grandisLinn. f.) and Ekor Kucing leaves (Acalypha hispidaBrum. f.) than determined itseffectiveness against Stahpylococcus aureusbacteria. This research was conducted by  make a liquid soap with various extract concentration  1%:4%,  4%:1%,  2,5%:2,5%,  2%:3%,  and  3%:2%,  furthermore  the specification of soap was tested for organoleptic, pH, high foam, moisture content, free  alkaline  content  and  specific  gravity.  Antibacterial  effectiveness  against  the growth  of Staphylococcus  aureuswas  done  bydiffusion  method.  The  results  of study show thatliquid soap preparations meet the requirements in accordance with SNI. The results obtained from the antibacterial effectiveness test of the liquid soap combination of ethanol extract of Teak leaves and Ekor Kucing leaves can inhibit Staphylococcus  aureusbacteria  with  all  the  concentrations  were  in  the  strong category,  with  the concentration  of 2,5%: 2,5%  which  has  the  largest  diameter namely 17,67 mm.Keywords  :     Antibacterial, Liquid Soap, Teak, Ekor Kucing.ABSTRAK Daun Jati (Tectona grandis Linn.f.) dan Daun Ekor Kucing (Acalypha hispida Brum.f.) merupakan tanaman yang memiliki kandungan senyawa metabolit sekunder yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasikan sabun cair kombinasi ekstrak etanol daun Jati dan daun Ekor Kucing dan pengujian efektivitas sediaan terhadap bakteri Staphylococcus aureus. Penelitian ini dilakukan dengan membuat sabun cair dengan variasi konsentrasi ekstrak yaitu 1%:4%, 4%:1%, 2,5%:2,5%, 2%:3%, dan 3%:2%, selanjutnya sabun diuji spesifikasinya meliputi pengujian organoleptik, pH, tinggi busa, kadar air, kadar alkali bebas, dan bobot jenis. Pengujian efektivitas antibakteri terhadap pertumbuhan Staphylococcus aureus dilakukan dengan menggunakan metode difusi. Hasil pengujian mutu sediaan sabun cair memenuhi persyaratan sesuai dengan SNI. Hasil uji efektivitas antibakteri sabun cair kombinasi ekstrak etanol daun Jati dan daun Ekor Kucing yang diperoleh dapat menghambat bakteri Staphylococcus aureus dengan semua konsentrasinya termasuk dalam kategori kuat, dengan konsentrasi 2,5%:2,5% yang memiliki diameter paling besar yaitu 17,67 mm.Kata Kunci  : Antibakteri, Sabun Cair, Jati, Ekor Kucing.

scholarly journals FORMULASI DAN UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN SABUN CAIR EKSTRAK ETANOL DAUN KERSEN (Muntingia Calabura L.) TERHADAP BAKTERI Staphlococcous epidermidis

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 30
Author(s):  
Freisy C.C Korompis ◽  
Paulina V. Y. Yamlean ◽  
Widya Astuty Lolo
Keyword(s):  
Specific Gravity ◽  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Bacterial Activity ◽  
Diffusion Method ◽  
Test Results ◽  
Alkali Content ◽  
Liquid Soap ◽  
Muntingia Calabura

ABSTRACT Cherry plants (Muntingia calabura L.) is a plant that has many properties in the treatment. One part of the plant that is widely used is the leaf which contain of flavonoid compounds, saponins and tannins contained in cherry leaves can inhibit bacterial activity. This study aims to formulate the liquid soap from ethanol extract of cherry leaves and test the antibacterial effectiveness of the liquid soap ethanol extract of cherry leaves with a concentration of 10%, 15% and 20% on the growth of Staphylococcus epidermidis bacteria. Liquid soap formulation of ethanol extract of cherry leaves with a concentration of 10%, 15% and 20% was conducted by laboratory experimental research. The results of testing the quality of liquid soap at a concentration of 10% meet the requirements according to the standards set by SNI namely organoleptic test, pH, high foam, moisture content, free alkali content, specific gravity. Concentrations of 15% and 20% did not meet the requirements for specific gravity testing. The test results of the antibacterial effectiveness of liquid soap ethanol extract of cherry leaves using the diffusion method with way of wells can inhibit the bacteria Staphylococcus epidermidis at concentrations of 10%, 15%, and 20% which categorize as weak. Keywords: Kersen, Peel-off mask, Antibacterial, Staphlococcous epidermidis  ABSTRAK Tanaman Kersen (Muntingia calabura L.) merupakan tanaman yang memiliki banyak khasiat dalam pengobatan. Salah satu bagian tanaman yang banyak digunakan ialah bagian daun yang mengandung senyawa flavonoid, saponin dan tannin yang terdapat pada daun kersen mampu menghambat aktivitas bakteri. Penelitian ini bertujuan untuk memformulasi sediaan sabun cair ekstrak etanol daun kersen dan  menguji efektivitas antibakteri sediaan sabun cair ekstrak etanol daun kersen dengan konsentrasi 10%, 15% dan 20% terhadap pertumbuhan bakteri Staphylococcus Epidermidis. Formulasi sabun cair ekstrak etanol daun kersen dengan konsentrasi 10%, 15% dan 20% dilakukan penelitian dengan metode eksperimental laboratorium. Hasil pengujian mutu sabun cair pada konsentrasi 10% memenuhi persyaratan sesuai standar yang ditetapkan SNI yaitu uji organoleptik, pH, tinggi busa, kadar air, kadar alkali bebas, bobot jenis. Konsentrasi 15% dan 20% tidak memenuhi persyaratan pada pengujian bobot jenis. Hasil uji efektivitas antibakteri sabun cair ekstrak etanol daun kersen menggunakan metode difusi agar dengan cara sumuran dapat menghambat bakteri Staphylococcus Epidermidis pada konsentrasi 10%, 15%, dan 20% yang termasuk dalam kategori lemah. Kata kunci : Kersen, Masker peel-off, Antibakteri, Staphlococcous epidermidis

scholarly journals LIQUID SOAP WITH ADDITIONS SARGASSUM (Sargassum polycystum) EXTRACT

2019 ◽  
Vol 1 (2) ◽  
pp. 71
Author(s):  
Allya Zahra ◽  
Irfan Restu Fauzan ◽  
Intan Oktavia D ◽  
Setyaningrum Setyaningrum ◽  
Sujuliyani Sujuliyani
Keyword(s):  
Specific Gravity ◽  
Moisture Content ◽  
Sodium Sulfate ◽  
Foam Stability ◽  
Raw Materials ◽  
Sensory Testing ◽  
Liquid Soap ◽  
Sargassum Polycystum ◽  
Biological Tests

Research on making Sargassum soap with the addition of Sargassum polycystum seaweed was carried out for 2 months at the Jakarta College of Fisheries. This study aims to determine the flow of the Sargassum polycystum soap making process, find out how to make Sargassum polycystum soap extracts, determine the quality of liquid soap produced through sensory, chemical and biological tests. This research uses direct research methods. Research results show that the process of making liquid soap extracts include: preparation of raw materials, maceration, evaporation, rinsing and drying. The process of making liquid soap, among others, by adding chemicals include: texafon S, sodium sulfate, aquades, texafon EVR, glycerin, olive oil Sargassum extract, perfume. Based on the results of sensory testing of soap by adding the concentration of Sargassum extract with soap that did not get the addition of Sargassum, it has an average rating that is almost the same. In addition to knowing the quality of Sargassum, chemical, microbiological and anti-bacterial tests were also carried out. Chemical tests include: Moisture content, specific gravity, viscosity, foam stability, Ph test, and free alkali. While the microbiological tests conducted on soap are: ALT.Keywords: Liquid soap, Seaweed, Sargassum, Extract

scholarly journals FORMULASI DAN UJI DAYA HAMBAT SABUN CAIR EKSTRAK ETANOL SERAI WANGI (Cymbopogon nardus L) TERHADAP PERTUMBUHAN Staplylococcus aureus

2021 ◽  
Vol 3 (1) ◽  
pp. 45-57
Author(s):  
Rinaldi Rinaldi ◽  
Fauziah Fauziah ◽  
Rizka Mastura
Keyword(s):  
Staphylococcus Aureus ◽  
Foam Stability ◽  
Foam Height ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Homogeneity Test ◽  
Liquid Soap ◽  
Ph Values ◽  
Test Parameters ◽  
Test Shape

Citronella (Cymbopogon Nardus.L) contains essential oil compounds, saponins, polyphenols and flavonoids which are antibacterial. The use of active compounds of Citronella is formulated into antibacterial liquid soap preparations. This study aims to determine the characteristics of liquid soap and its inhibition against the growth of Staphylococcus aureus. This research is experimental to formulate liquid soap containing ethanol extract of lemongrass sticks at concentrations of 9% (F1), 18% (F2) and 24% (F3). The test parameters for liquid soap preparations include organoleptic test (shape, aroma, color), homogeneity test, pH test, foam height test, foam stability test, free alkaline test and inhibition test against the growth of Staphylococcus aureus using the Kirby-Bauer method. The results showed that the liquid soap preparations in the F1, F2 and F3 formulas were liquid, had a distinctive aroma of Citronella and were brown in color, homogeneous. The pH values, foam height, foam stability and free alkaline content of liquid soap were F1 (12; 70 mm; 85.7% and 0.14%), F2 (11. 80 mm, 87.5% and respectively). 0.10%), F3 (10.100 mm, 90% and 0.08%). The formula inhibition zone diameters are 32.06 mm, 25.6 mm and 29.2 mm, respectively. The conclution is the characteristics of the liquid soap with the ethanol extract of Citronella (Cymbopogon Nardus.L) meet the requirements as liquid soap. The greatest inhibitory effectiveness on the growth of Staphylococcus aureus was a formula at a concentration of 24% (F3) with a strong category. 

scholarly journals FORMULASI SEDIAAN SABUN CAIR KOMBINASI EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) DAN DAUN KEMANGI (Ocymum basilicum L.) DAN UJI TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 404
Author(s):  
Dwi A.K. Nau'e ◽  
Paulina V. Y. Yamlean ◽  
Deby A. Mpila
Keyword(s):  
Staphylococcus Aureus ◽  
Specific Gravity ◽  
Experimental Method ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Alkali Content ◽  
Liquid Soap ◽  
Measurement Height ◽  
Dark Green ◽  
Muntingia Calabura

ABSTRACTKersen leaves (Muntingia Calabura L.) and Basil leaves (Ocymum basilicum L.) are plants that contain flavonoid compounds, saponin and tannin which acts as antibacterial. The aims of this study is formulate liquid soap in combination of ethanol extract of Kersen leaves and basil leaves and to test the antibacterial effectiveness and evaluate the physical preparates of liquid soap in combination of ethanol extract of Kersen leaves and Kemangi leaves. This research uses a laboratorium experimental method. Based on the results of the study showed physical evaluations such as organoleptics which are dark green and dark brown, Soap pH above 8, measurement height of foam 60-88 mm, calculation of water content was 46%, free alkali content was 0,05-0,06 g/ml, and specific gravity was 1,01-1,09 g/ml. Liquid soap combination of Kersen and Basil leaves extract has antibacterial effectiveness of average diameter for FI 10 mm, F2 10,33 mm, F3 11 mm, F4 11,5 mm, and F5 10,67 mm. In conclusion, the combination of Kersen and Basil leaves extract can be formulated into liquid soap, liquid soap preparations in physical evaluation testing had met the standards set by SNI. Liquid soap preparation has antibacterial effectiveness which is included in the strong category.Keywords: Antibacterial, Liquid Soap, Kersen, Basil.ABSTRAK Daun Kersen (Muntingia Calabura L.) dan Daun Kemangi (Ocymum basilicum L.) merupakan tanaman yang memiliki kandungan senyawa flavonoid, saponin dan tannin yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasikan sabun cair kombinasi ekstrak etanol daun Kersen dan daun Kemangi serta menguji efektivitas antibakteri dan mengevaluasi sediaan fisik dari sabun cair kombinasi ekstrak etanol daun Kersen dan daun Kemangi. Penelitian ini menggunakan metode ekperimental laboratorium. Berdasarkan hasil penelitian menunjukkan evaluasi fisik seperti organoleptik berwarna hijau tua dan cokelat tua, pH sabun diatas 8, pengukuran tinggi busa 60-88 mm, perhitungan kadar air yaitu 46%, kadar alkali bebas yaitu 0,05-0,06 g/ml, dan bobot jenis yaitu 1,01-1,09 g/ml. Sediaan sabun cair kombinasi ekstrak daun Kersen dan daun Kemangi memiliki efektifitas antibakteri diameter rata-rata untuk FI 10 mm, F2 10,33 mm, F3 11 mm, F4 11,5 mm, dan F5 10,67 mm. Kesimpulannya kombinasi ekstrak daun Kersen dan daun Kemangi dapat diformulasikan menjadi sabun cair, sediaan sabun cair dalam pengujian evaluasi fisik telah memenuhi standar yang ditetapkan oleh SNI. Sediaan sabun cair memiliki efektifitas antibakteri yang termasuk kategori kuat. Kata Kunci: Antibakteri, Sabun Cair, Kersen, Kemangi.

scholarly journals UJI EFEKTIVIAS ANTIBAKTERI SEDIAAN SABUN CAIR KOMBINASI EKSTRAK ETANOL DAUN JATI (Tectona grandis L.) DAN BUNGA PACAR AIR (Impantiens balsamina L.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 622
Author(s):  
Sabine A. Nelwan ◽  
Paulina V.Y. Yamlean ◽  
Jainer Pasca Siampa
Keyword(s):  
Staphylococcus Aureus ◽  
Moisture Content ◽  
Tectona Grandis ◽  
Experimental Methods ◽  
National Standard ◽  
Diffusion Method ◽  
Liquid Soap ◽  
Antibacterial Drugs ◽  
Organoleptic Test ◽  
Quality Testing

ABSTRACTJati Leaves (Tectona grandis L.) and Rose balsam flower (Impantiens balsamina L.) contain flavonoid compounds, saponins and tannins that are antibacterial. This research was aimed to formulate liquid soap extracts of Jati leaves and Rose balsam flower ethanol and test the antibacterial drugs for the antibacterial liquid soap with a concentration of (6%:2%), (5%:3%), (4%:4%). (3%:5%) and (2%:6%) against Staphylococcus aureus bacteria. Experimental methods were permorfed for this research. For the formulation of this liquid soap, several experiments were conducted (e.g., organoleptic test, pH, high foam, moisture content, free alkaline content, and type weights). The effectiveness of antibacterial to s.aureus growth was tested by diffusion method. The results of liquid soap quality testing finally met all the requirements according to ISN (Indonesian National Standard). The results of the effectiveness of antibacterial liquid soap esxtract of the obtained Jati leaves and Rose balsam flower can inhibit the s.aureus bacteria, with a concentration of (5%:3%) and (2%:6%) which has the strongest inhibition with a diameter of 19,3 mm. Keywords: Jati Leaves, Rose Balsam, Liquid Soap, Antibacterial.  ABSTRAKDaun Jati (Tectona grandis L.) dan Bunga Pacar Air (Impantiens balsamina L.) memiliki kandungan senyawa flavonoid, saponin, dan tannin yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasikan sabun cair kombinasi ekstrak etanol daun Jati dan bunga Pacar Air serta menguji aktivitas antibakteri sediaan sabun cair dengan konsentrasi (6%:2%), (5%:3%), (4%:4%), (3%:5%), (2%:6%) terhadap pertumbuhan bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental. Formulasi sediaan sabun cair kombinasi dilakukan pengujian organoleptic, pH, tinggi busa, kadar air, kadar alkali bebas, dan bobot jenis. Pengujian aktivitas antibakteri dilakukan dengan metode difusi. Hasil pengujian mutu sabun cair memenuhi standar yang ditetapkan SNI (Standar Nasional Indonesia). Hasil uji aktivitas antibakteri yang diperoleh dapat menghambat bakteri s.aureus dengan konsentrasi (5%:3%) dan (2%:6%) yang memiliki daya hambat terkuat dengan diameter 19,3 mm. Kata kunci: Daun Jati, Bunga Pacar Air, Sabun Cair, Antibakteri.

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

scholarly journals Growth performance and wood quality of white jabon (Neolamarckia cadamba) progeny testing at Parung Panjang, Bogor, Indonesia

2019 ◽  
Vol 20 (8) ◽  
Author(s):  
Nurul Chaerani ◽  
DEDE J SUDRAJAT ◽  
ISKANDAR Z SIREGAR ◽  
ULFAH J SIREGAR
Keyword(s):  
Specific Gravity ◽  
Moisture Content ◽  
Growth Performance ◽  
Wood Density ◽  
Block Design ◽  
Wood Quality ◽  
Basic Density ◽  
Progeny Testing ◽  
Pilodyn Penetration

Abstract. Chaerani N, Sudrajat DJ, Siregar IZ, Siregar UJ. 2019. Growth performance and wood quality of white jabon (Neolamarckia cadamba) progeny testing at Parung Panjang, Bogor, Indonesia. Biodiversitas 20: 2295-2301. The aim of this study was to evaluate the genetic parameters of growth and wood quality in white jabon progeny test at 54 months old in Parung Panjang, Bogor. The 105 half-sib families obtained from 12 provenances were evaluated in a randomized complete block design with five replications. Wood quality was assessed both in a non-destructive way using a pilodyn and by destructive method using wood sample taker. Results indicated that the mean value ranged from 5.10 to 10.15 m for height, 6.67 to 15.30 cm for diameter, 2.30 to 3.62 cm for pilodyn penetration, 0.66 to 0.82 g/cm3 for wood density, 0.33 to 0.50 for specific gravity, and 66 to 111 % for moisture content, respectively. There were significant differences among 105 families for all traits except moisture content. The high heritability estimate was found for height (0.4-0.69) and basic density (0.27-0.59). Applying 80% selection intensity on diameter and leaving 84 best families in each block will produce a high total genetic gain. Pilodyn penetration had negative correlation with diameter, wood density, and specific gravity.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Muhtadi , MSi. ◽  
Ria Ambarwati ◽  
Ratna Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

2016 ◽  
Vol 6 (2) ◽  
pp. 70-76
Author(s):  
Soumia Keddari ◽  
Narimen Benaoum ◽  
Yasmina Mokhtaria Boufadi ◽  
Mansouria Belhocine ◽  
Ali Riazi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Ammi Visnaga ◽  
Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

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