FORMULASI SEDIAAN SABUN CAIR KOMBINASI EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) DAN DAUN KEMANGI (Ocymum basilicum L.) DAN UJI TERHADAP BAKTERI Staphylococcus aureus

ABSTRACTKersen leaves (Muntingia Calabura L.) and Basil leaves (Ocymum basilicum L.) are plants that contain flavonoid compounds, saponin and tannin which acts as antibacterial. The aims of this study is formulate liquid soap in combination of ethanol extract of Kersen leaves and basil leaves and to test the antibacterial effectiveness and evaluate the physical preparates of liquid soap in combination of ethanol extract of Kersen leaves and Kemangi leaves. This research uses a laboratorium experimental method. Based on the results of the study showed physical evaluations such as organoleptics which are dark green and dark brown, Soap pH above 8, measurement height of foam 60-88 mm, calculation of water content was 46%, free alkali content was 0,05-0,06 g/ml, and specific gravity was 1,01-1,09 g/ml. Liquid soap combination of Kersen and Basil leaves extract has antibacterial effectiveness of average diameter for FI 10 mm, F2 10,33 mm, F3 11 mm, F4 11,5 mm, and F5 10,67 mm. In conclusion, the combination of Kersen and Basil leaves extract can be formulated into liquid soap, liquid soap preparations in physical evaluation testing had met the standards set by SNI. Liquid soap preparation has antibacterial effectiveness which is included in the strong category.Keywords: Antibacterial, Liquid Soap, Kersen, Basil.ABSTRAK Daun Kersen (Muntingia Calabura L.) dan Daun Kemangi (Ocymum basilicum L.) merupakan tanaman yang memiliki kandungan senyawa flavonoid, saponin dan tannin yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasikan sabun cair kombinasi ekstrak etanol daun Kersen dan daun Kemangi serta menguji efektivitas antibakteri dan mengevaluasi sediaan fisik dari sabun cair kombinasi ekstrak etanol daun Kersen dan daun Kemangi. Penelitian ini menggunakan metode ekperimental laboratorium. Berdasarkan hasil penelitian menunjukkan evaluasi fisik seperti organoleptik berwarna hijau tua dan cokelat tua, pH sabun diatas 8, pengukuran tinggi busa 60-88 mm, perhitungan kadar air yaitu 46%, kadar alkali bebas yaitu 0,05-0,06 g/ml, dan bobot jenis yaitu 1,01-1,09 g/ml. Sediaan sabun cair kombinasi ekstrak daun Kersen dan daun Kemangi memiliki efektifitas antibakteri diameter rata-rata untuk FI 10 mm, F2 10,33 mm, F3 11 mm, F4 11,5 mm, dan F5 10,67 mm. Kesimpulannya kombinasi ekstrak daun Kersen dan daun Kemangi dapat diformulasikan menjadi sabun cair, sediaan sabun cair dalam pengujian evaluasi fisik telah memenuhi standar yang ditetapkan oleh SNI. Sediaan sabun cair memiliki efektifitas antibakteri yang termasuk kategori kuat. Kata Kunci: Antibakteri, Sabun Cair, Kersen, Kemangi.

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FORMULASI DAN UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN SABUN CAIR EKSTRAK ETANOL DAUN KERSEN (Muntingia Calabura L.) TERHADAP BAKTERI Staphlococcous epidermidis

PHARMACON ◽

10.35799/pha.9.2020.27407 ◽

2020 ◽

Vol 9 (1) ◽

pp. 30

Author(s):

Freisy C.C Korompis ◽

Paulina V. Y. Yamlean ◽

Widya Astuty Lolo

Keyword(s):

Specific Gravity ◽

Staphylococcus Epidermidis ◽

Ethanol Extract ◽

Bacterial Activity ◽

Diffusion Method ◽

Test Results ◽

Alkali Content ◽

Liquid Soap ◽

Muntingia Calabura

ABSTRACT Cherry plants (Muntingia calabura L.) is a plant that has many properties in the treatment. One part of the plant that is widely used is the leaf which contain of flavonoid compounds, saponins and tannins contained in cherry leaves can inhibit bacterial activity. This study aims to formulate the liquid soap from ethanol extract of cherry leaves and test the antibacterial effectiveness of the liquid soap ethanol extract of cherry leaves with a concentration of 10%, 15% and 20% on the growth of Staphylococcus epidermidis bacteria. Liquid soap formulation of ethanol extract of cherry leaves with a concentration of 10%, 15% and 20% was conducted by laboratory experimental research. The results of testing the quality of liquid soap at a concentration of 10% meet the requirements according to the standards set by SNI namely organoleptic test, pH, high foam, moisture content, free alkali content, specific gravity. Concentrations of 15% and 20% did not meet the requirements for specific gravity testing. The test results of the antibacterial effectiveness of liquid soap ethanol extract of cherry leaves using the diffusion method with way of wells can inhibit the bacteria Staphylococcus epidermidis at concentrations of 10%, 15%, and 20% which categorize as weak. Keywords: Kersen, Peel-off mask, Antibacterial, Staphlococcous epidermidis ABSTRAK Tanaman Kersen (Muntingia calabura L.) merupakan tanaman yang memiliki banyak khasiat dalam pengobatan. Salah satu bagian tanaman yang banyak digunakan ialah bagian daun yang mengandung senyawa flavonoid, saponin dan tannin yang terdapat pada daun kersen mampu menghambat aktivitas bakteri. Penelitian ini bertujuan untuk memformulasi sediaan sabun cair ekstrak etanol daun kersen dan menguji efektivitas antibakteri sediaan sabun cair ekstrak etanol daun kersen dengan konsentrasi 10%, 15% dan 20% terhadap pertumbuhan bakteri Staphylococcus Epidermidis. Formulasi sabun cair ekstrak etanol daun kersen dengan konsentrasi 10%, 15% dan 20% dilakukan penelitian dengan metode eksperimental laboratorium. Hasil pengujian mutu sabun cair pada konsentrasi 10% memenuhi persyaratan sesuai standar yang ditetapkan SNI yaitu uji organoleptik, pH, tinggi busa, kadar air, kadar alkali bebas, bobot jenis. Konsentrasi 15% dan 20% tidak memenuhi persyaratan pada pengujian bobot jenis. Hasil uji efektivitas antibakteri sabun cair ekstrak etanol daun kersen menggunakan metode difusi agar dengan cara sumuran dapat menghambat bakteri Staphylococcus Epidermidis pada konsentrasi 10%, 15%, dan 20% yang termasuk dalam kategori lemah. Kata kunci : Kersen, Masker peel-off, Antibakteri, Staphlococcous epidermidis

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FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI SABUN CAIR KOMBINASI EKSTRAK ETANOL DAUN JATI (Tectona grandis Linn.f.) DAN DAUN EKOR KUCING (Acalypha hispida burm.f.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽

10.35799/pha.9.2020.31366 ◽

2020 ◽

Vol 9 (4) ◽

pp. 558

Author(s):

Nia Kartika Pareda ◽

Hosea J. Edy ◽

Julianri S. Lebang

Keyword(s):

Staphylococcus Aureus ◽

Specific Gravity ◽

Moisture Content ◽

Secondary Metabolite ◽

Ethanol Extract ◽

Tectona Grandis ◽

Liquid Soap ◽

Extract Concentration

ABSTRACTTeak leaves (Tectona grandisLinn. f.) and Ekor Kucing leaves (Acalypha hispida Brum. f.) are plants that contain secondary metabolite compounds that can inhibit bacteria. This research aimsisto formulate liquid soap containethanol extract of Teak leaves (Tectona grandisLinn. f.) and Ekor Kucing leaves (Acalypha hispidaBrum. f.) than determined itseffectiveness against Stahpylococcus aureusbacteria. This research was conducted by make a liquid soap with various extract concentration 1%:4%, 4%:1%, 2,5%:2,5%, 2%:3%, and 3%:2%, furthermore the specification of soap was tested for organoleptic, pH, high foam, moisture content, free alkaline content and specific gravity. Antibacterial effectiveness against the growth of Staphylococcus aureuswas done bydiffusion method. The results of study show thatliquid soap preparations meet the requirements in accordance with SNI. The results obtained from the antibacterial effectiveness test of the liquid soap combination of ethanol extract of Teak leaves and Ekor Kucing leaves can inhibit Staphylococcus aureusbacteria with all the concentrations were in the strong category, with the concentration of 2,5%: 2,5% which has the largest diameter namely 17,67 mm.Keywords : Antibacterial, Liquid Soap, Teak, Ekor Kucing.ABSTRAK Daun Jati (Tectona grandis Linn.f.) dan Daun Ekor Kucing (Acalypha hispida Brum.f.) merupakan tanaman yang memiliki kandungan senyawa metabolit sekunder yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasikan sabun cair kombinasi ekstrak etanol daun Jati dan daun Ekor Kucing dan pengujian efektivitas sediaan terhadap bakteri Staphylococcus aureus. Penelitian ini dilakukan dengan membuat sabun cair dengan variasi konsentrasi ekstrak yaitu 1%:4%, 4%:1%, 2,5%:2,5%, 2%:3%, dan 3%:2%, selanjutnya sabun diuji spesifikasinya meliputi pengujian organoleptik, pH, tinggi busa, kadar air, kadar alkali bebas, dan bobot jenis. Pengujian efektivitas antibakteri terhadap pertumbuhan Staphylococcus aureus dilakukan dengan menggunakan metode difusi. Hasil pengujian mutu sediaan sabun cair memenuhi persyaratan sesuai dengan SNI. Hasil uji efektivitas antibakteri sabun cair kombinasi ekstrak etanol daun Jati dan daun Ekor Kucing yang diperoleh dapat menghambat bakteri Staphylococcus aureus dengan semua konsentrasinya termasuk dalam kategori kuat, dengan konsentrasi 2,5%:2,5% yang memiliki diameter paling besar yaitu 17,67 mm.Kata Kunci : Antibakteri, Sabun Cair, Jati, Ekor Kucing.

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UJI AKTIVITAS ANTIBAKTERI SEDIAAN SABUN CAIR EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽

10.35799/pha.10.2021.32784 ◽

2021 ◽

Vol 10 (1) ◽

pp. 767

Author(s):

Evander Roliand Lomboan ◽

Paulina V.Y. Yamlean ◽

Elly J. Suoth

Keyword(s):

Staphylococcus Aureus ◽

Specific Gravity ◽

Moisture Content ◽

Foam Height ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Diffusion Method ◽

Syzygium Aromaticum ◽

Liquid Soap

ABSTRACT Clove leaf (Syzygium aromaticum) is a plant that contains eugenol, saponins, flavonoids and tannins which have antibacterial properties. This study aims to formulate the ethanol extract liquid soap of clove leaves and test the effectiveness of the preparation against Staphylococcus aureus bacteria. This research was conducted in a laboratory experiment to make liquid soap with extract concentrations of 4%, 7%, 9%, 12% and 15%, then the soap was tested for spesifications including organoleptic testing, pH, foam height, moisture content, free alkaline content, and specific gravity. Antibacterial effectiveness testing against the growth of Staphylococcus aureus was carried out using the diffusion method. Clove leaf ethanol extract (Syzygium aromaticum) can be formulated into liquid soap. The results of testing the quality of liquid soap preparations meet the requirements in accordance with SNI. The results obtained from the antibacterial effectiveness test of liquid soap ethanol extract of Clove leaves can inhibit Staphylococcus aureus bacteria with all concentrations in the strong category, with a concentration of 15% having the largest diameter of 17,33 mm. Keywords : Antibacterial, Liquid Soap, Syzygium aromaticum, Staphylococcus aureus ABSTRAK Daun Cengkeh (Syzygium aromaticum) merupakan tanaman yang memiliki kandungan eugenol, saponin, flavonoid dan tannin yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk memformulasi sabun cair ekstrak etanol daun Cengkeh dan pengujian efektivitas sediaan terhadap bakteri Stahpylococcus aureus. Penelitian ini dilakukan secara eksperimental laboratorium untuk membuat sabun cair dengan konsentrasi ekstrak yaitu 4%, 7%, 9%, 12%, dan 15%, selanjutnya sabun diuji spesifikasinya meliputi pengujian organoleptik, pH, tinggi busa, kadar air, kadar alkali bebas, dan bobot jenis. Pengujian efektivitas antibakteri terhadap pertumbuhan Staphylococcus aureus dilakukan dengan menggunakan metode difusi. Ekstrak etanol daun Cengkeh (Syzygium aromaticum) dapat diformulasikan menjadi sabun cair. Hasil pengujian mutu sediaan sabun cair memenuhi persyaratan sesuai dengan SNI. Hasil uji efektivitas antibakteri sabun cair ekstrak etanol daun Cengkeh yang diperoleh dapat menghambat bakteri Staphylococcus aureus dengan semua konsentrasinya termasuk dalam kategori kuat, dengan konsentrasi 15% yang memiliki diameter paling besar yaitu 17,33 mm. Kata Kunci: Antibakteri, Sabun Cair, Syzygium aromaticum , Staphylococcus aureus

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Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus

Medicra (Journal of Medical Laboratory Science/Technology) ◽

10.21070/medicra.v3i2.760 ◽

2020 ◽

Vol 3 (2) ◽

pp. 36-40

Author(s):

Viki Ayu Intan Permatasari ◽

Mutia Hariani Nurjanah ◽

Wimbuh Tri Widodo

Keyword(s):

Staphylococcus Aureus ◽

Aloe Vera ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Positive Control ◽

Negative Control ◽

Traditional Medicines ◽

Alternative Ingredients ◽

High Concentrations

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations

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EFEKTIVITAS GEL EKSTRAK ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus

Jurnal Riset Kefarmasian Indonesia ◽

10.33759/jrki.v2i2.84 ◽

2020 ◽

Vol 2 (2) ◽

pp. 105-112

Author(s):

Irene Puspa Dewi ◽

Ilham Maslan Orde ◽

Verawaty Verawaty

Keyword(s):

Staphylococcus Aureus ◽

Allium Sativum ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Traditional Medicines ◽

Significant Difference ◽

Irritation Test ◽

Gel Preparation ◽

The One

Garlic (Allium sativum L.) is one of the traditional medicines which has an antibacterial efficacy compound namely Allisin which is able to inhibit the growth of Staphylococcus aureus bacteria. This study aims to formulate the garlic bulb extract into a good gel preparation for use as an acne drug and to determine the antibacterial effectiveness of the ethanol extract of garlic gel against the bacterium Staphylococcus aureus using the wells method. The gel evaluation was carried out to meet the requirements with organoleptic test parameters, homogeneity, pH, spreadability, adhesion and irritation test. This study uses extracts of 10% and 20% concentrations, at each concentration being able to inhibit the growth of Staphylococcus aureus bacteria. In the formula, the concentration of 20% has a inhibition zone diameter of 1.59 cm and a concentration of 10% has a inhibition of 1.50 cm. Statistical test results using the One Way ANOVA test found that there was no significant difference between the average diameter of inhibition of garlic extract gel concentration of 10% with a concentration of 20%.

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Uji Aktivitas Ekstrak Etanolik Daun Kemangi (Ocimum sanctum L.) terhadap Staphylococcus aureus secara In Vitr

Jurnal Pharmascience ◽

10.20527/jps.v7i1.8080 ◽

2020 ◽

Vol 7 (1) ◽

pp. 107

Author(s):

Novia Ariani ◽

Dwi Rizki Febrianti ◽

Rakhmadhan Niah

Keyword(s):

Staphylococcus Aureus ◽

Sampling Technique ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Ocimum Sanctum ◽

Purposive Sampling ◽

Extract Concentration

ABSTRAK Tanaman kemangi banyak dimanfaatkan oleh masyarakat untuk pengobatan infeksi khususnya bagian daun. Hal ini dikarenakan daun kemangi memiliki senyawa aktif seperti minyak atsiri, alkaloid, saponin, flavonoid, triterpenoid, steroid, tannin dan fenol yang dapat menghambat pertumbuhan bakteri. Tujuan penelitian ini dilakukan untuk mengetahui ada tidaknya aktivitas, mengetahui diameter zona hambat dan mengetahui klasifikasi kekuatan aktivitas daya hambat antibakteri ekstrak etanol daun kemangi. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi lubang sumuran dengan teknik pengambilan sampel adalah purposive sampling. Konsentrasi ekstrak yang digunakan yaitu konsentrasi 100%, 80%, 60%, 40%, 20%, sedangkan untuk kontrol positif digunakan klindamisin 30µg, dan kontrol negatif yang digunakan etanol 96%. Hasil diameter zona hambat yang terbentuk diukur dengan jangka sorong. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kemangi memiliki aktivitas dalam menghambat pertumbuhan bakteri Staphylococcus aureus dengan diameter rata-rata yang didapat dari setiap perlakuan yaitu 100% (10,08 mm), 80% (8,10 mm), 60% (6,49 mm), 40% (4,29 mm), 20% (2,26 mm), dan sebagai klasifikasi kekuatan aktivitas daya hambat antibakteri yaitu pada konsentrasi 100% kuat, 80%-60% sedang dan 40%-20% lemah. Kata Kunci : Daun kemangi, Ekstrak, Difusi, Staphylooccus aureus ABSTRACT Part of the basil plant (Ocimum sanctum L.) that widely used by people for treatment of infections is basil leaves. This is because basil leaves have active compounds such as essential oils, alkaloids, saponins, flavonoids, triterpenoids, steroids, tannins and phenols which can inhibit bacterial growth. This research aimed to find out the presence or absence of activity, to determine the diameter of the inhibitory zone and the classification of antibacterial mention against what the name of bacterial is activity of ethanol extract of basil leaves. The type of this research is experimental research with a well diffusion method with sampling technique is purposive sampling. The concentration of extracts used were concentrations of 100%, 80%, 60%, 40%, 20%, while as positive control is clindamycin 30µg, and the negative control used 96% ethanol. The resulting diameter of the inhibition zone is measured by the calipers. The results showed that the ethanol extract of basil leaves had an activity in inhibiting the growth of Staphylococcus aureus bacteria with an average diameter obtained from each treatment that was 100% (10,08mm); 80% (8,10mm); 60% (6,49mm); 40% (4,29mm); 20% (2,26mm), and as the antibacterial activity classification, that were strong in 100% of extract concentration, medium in 60-80% of extract concentration, and weak in 20-40% of extract concentration. Keywords : Basil leaf, Extract, Diffusion, Staphylooccus aureus

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The Characteristics of Liquid Soap with Additional Variations of Moringa Seed Extract (Moringa oleifera L.)

International Journal on Food, Agriculture and Natural Resources ◽

10.46676/ij-fanres.v2i3.43 ◽

2021 ◽

Vol 2 (3) ◽

pp. 5-11

Author(s):

Andrew Setiawan Rusdianto ◽

Atika Yulianti ◽

Sony Suwasono ◽

Andi Eko Wiyono

Keyword(s):

Specific Gravity ◽

Moringa Oleifera ◽

Ph Value ◽

Foam Stability ◽

Seed Extract ◽

Free Alkali ◽

Alkali Content ◽

Liquid Soap ◽

Anova Analysis ◽

Foaming Power

Soap is a cleaning compound formed from the reaction between fatty acid compounds and alkaline bases. Various types of soap have been circulating in the market, one of which is liquid soap. Liquid soap that has antibacterial abilities is needed by many people. Moringa seed extract is one of the natural ingredients that can be used as an active ingredient in making liquid soap because it can inhibit bacterial growth. This study aims to determine the effect of variations in the addition of extracts and to obtain the best extract concentration treatment on physical, chemical, and organoleptic. The addition of moringa seed extract used was administered at 3%, 5%, and 7%. Data analysis was carried out by applying the ANOVA (Analysis of Variance) with a significant level of 5%. The De Garmo method was used to formulate the best liquid soap recommendation among various treatments. The results showed that the addition of moringa seed extract had a significant effect on the parameters of free alkali content, viscosity, specific gravity, antibacterial activity, and organoleptic. The best recommendation for liquid soap is a soap with a concentration of 7% with specific gravity characteristics of 1.066 g/ml, the viscosity of 1.983 cP, pH value of 10.53, foaming power of 5.63 cm, foam stability of 94.64%, and free alkali content of 0.023%, and the antibacterial ability against S. aureus bacteria (clear zone) was 19.17 mm.

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Uji Daya Hambat Ekstrak Etanol Batang Tumbuhan Saluang Belum Terhadap Bakteri Staphylococcus aureus

Jurnal Surya Medika ◽

10.33084/jsm.v4i1.168 ◽

2018 ◽

Vol 4 (1) ◽

pp. 90-101 ◽

Cited By ~ 2

Author(s):

Nurul Qamariah ◽

Rezqi Handayani ◽

Andika Friskila

Keyword(s):

Staphylococcus Aureus ◽

Minimum Inhibitory Concentration ◽

Medicinal Plants ◽

Experimental Method ◽

Inhibitory Concentration ◽

Ethanol Extract ◽

Inhibitory Zone ◽

Central Kalimantan ◽

The People ◽

Mic Minimum Inhibitory Concentration

One of the medicinal plants used by the people of Katingan Regency, Central Kalimantan Province is the Saluang Belum stem, which empirically used as an aphrodisiac to increase stamina, sexual and fertility of male, and as an antioxidant. The purpose of this study was to find out the inhibition and to know which concentration of ethanol extract of Saluang Belum stem which had been able to inhibit the growth of Staphylococcus aureus bacteria. This research uses an experimental method with a laboratory approach. The results of the inhibitory zone of ethanol extract of Saluang Belum stem at a concentration of 0.5% was 26.7 � 2.76 mm, at a concentration of 1% was 21.6 � 2.20 mm, at a concentration of 5% was 20.5 � 0, 90 mm, at 10% concentration was 21.2 � 1.11 mm, at 15% concentration was 23.2 � 0.23 mm, and at 20% concentration was 25.5 � 0.36 mm. Conclusions from this study were that the MIC (Minimum Inhibitory Concentration) obtained at 0.5% concentration of ethanol extract Batang Saluang Belum was able to inhibit the Staphylococcus aureus bacteria.

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FORMULASI DAN UJI DAYA HAMBAT SABUN CAIR EKSTRAK ETANOL SERAI WANGI (Cymbopogon nardus L) TERHADAP PERTUMBUHAN Staplylococcus aureus

Jurnal Riset Kefarmasian Indonesia ◽

10.33759/jrki.v3i1.115 ◽

2021 ◽

Vol 3 (1) ◽

pp. 45-57

Author(s):

Rinaldi Rinaldi ◽

Fauziah Fauziah ◽

Rizka Mastura

Keyword(s):

Staphylococcus Aureus ◽

Foam Stability ◽

Foam Height ◽

Ethanol Extract ◽

Inhibition Zone ◽

Homogeneity Test ◽

Liquid Soap ◽

Ph Values ◽

Test Parameters ◽

Test Shape

Citronella (Cymbopogon Nardus.L) contains essential oil compounds, saponins, polyphenols and flavonoids which are antibacterial. The use of active compounds of Citronella is formulated into antibacterial liquid soap preparations. This study aims to determine the characteristics of liquid soap and its inhibition against the growth of Staphylococcus aureus. This research is experimental to formulate liquid soap containing ethanol extract of lemongrass sticks at concentrations of 9% (F1), 18% (F2) and 24% (F3). The test parameters for liquid soap preparations include organoleptic test (shape, aroma, color), homogeneity test, pH test, foam height test, foam stability test, free alkaline test and inhibition test against the growth of Staphylococcus aureus using the Kirby-Bauer method. The results showed that the liquid soap preparations in the F1, F2 and F3 formulas were liquid, had a distinctive aroma of Citronella and were brown in color, homogeneous. The pH values, foam height, foam stability and free alkaline content of liquid soap were F1 (12; 70 mm; 85.7% and 0.14%), F2 (11. 80 mm, 87.5% and respectively). 0.10%), F3 (10.100 mm, 90% and 0.08%). The formula inhibition zone diameters are 32.06 mm, 25.6 mm and 29.2 mm, respectively. The conclution is the characteristics of the liquid soap with the ethanol extract of Citronella (Cymbopogon Nardus.L) meet the requirements as liquid soap. The greatest inhibitory effectiveness on the growth of Staphylococcus aureus was a formula at a concentration of 24% (F3) with a strong category.

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FORMULASI DAN UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN GEL EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI ANTISEPTIK TANGAN

PHARMACON ◽

10.35799/pha.8.2019.29335 ◽

2019 ◽

Vol 8 (3) ◽

pp. 580

Author(s):

Thesya Manarisip ◽

Paulina V.Y Yamlean ◽

Widya Astuty Lolo

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Average Diameter ◽

Clear Zone ◽

Leaf Extracts ◽

Cycling Test ◽

Power Testing ◽

Gel Preparation ◽

Colony Counter ◽

Muntingia Calabura

Kerson Fruit (Muntingia calabura L.) contains bioactive compounds, such as flavonoids, saponins, triterpenes, steroids, and tannins, which are potentially as antibacterial compounds. The aim of this study was to make a formulation of hand antiseptic gel from kerson leaf extracts with three variations in extract concentrations of 5%, 10%, and 15%, and to test the effectiveness of antibacterial preparations against Staphylococcus aureus bacteria. The method of this research is laboratory experimental research. Kerson extracts was obtained by maceration using 96% ethanol. The results showed that kerson leaf extract can be formulated as a hand antiseptic gel preparation that meets organoleptic requirements, like homogeneity, pH, dispersion, consistency, adhesion, cycling test, and antiseptic power testing using a colony counter. On the results of antibacterial effectiveness testing, there is a clear zone that presents the ability to inhibit the growth of gel test bacteria. The average diameter of the hand antiseptic gel preparation of kersen leaf extract at concentrations of 5%, 10%, and 15% respectively were 10.00 mm, 11.66 mm and 12.00 mm so that the ability of inhibition of gel test bacteria in all concentrations was categorized strong. Keywords: Kerson, Muntingia calabura L, Hand Antiseptic Gel, Staphylococcus aureus. ABSTRAK Kersen (Muntigia calabura L.) mengandung senyawa bioaktif yaitu senyawa flavonoid, saponin, triterpen, steroid, dan tannin yang merupakan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk membuat formulasi sediaan gel antiseptik tangan dari ekstrak daun kersen dengan tiga variasi konsentrasi ekstrak yakni 5%, 10%, dan 15%, serta menguji efektivitas antibakteri sediaan terhadap bakteri Staphylococcus aureus. Metode penelitian ini merupakan penelitian eksperimental laboratorium. Ekstrak tanaman kersen diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Hasil penelitian menunjukkan bahwa ekstrak daun kersen dapat diformulasikan sebagai sediaan gel antiseptik tangan yang memenuhi persyaratan organoleptik, homogenitas, pH, daya sebar, konsistensi, daya lekat, cycling test, dan pengujian daya antiseptik dengan menggunakan alat colony counter. Pada hasil pengujian efektivitas antibakteri, terdapat zona bening yang mempresentasikan kemampuan penghambatan pertumbuhan bakteri uji oleh gel. Diameter rata-rata sediaan gel antiseptik tangan ekstrak daun kersen pada konsentrasi 5%, 10%, dan 15% berturut-turut yaitu 10,00 mm , 11,66 mm dan 12,00 mm sehingga kemampuan penghambatan bakteri uji oleh gel disemua konsentrasi dikategorikan kuat. Kata kunci: Kersen, Muntingia calabura L, Gel Antiseptik Tangan, Staphylococcus aureus.

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