scholarly journals Antibacterial potential of marine fungus Aspergillus nomius isolated from green algae Bornetella sp

2021 ◽  
Vol 9 (1) ◽  
pp. 49
Author(s):  
Deiske Adeliene Sumilat ◽  
Rosita A. J. Lintang
Keyword(s):  
Staphylococcus Aureus ◽  
Green Algae ◽  
Marine Fungi ◽  
Marine Fungus ◽  
Diffusion Method ◽  
Antibacterial Compounds ◽  
Aspergillus Nomius ◽  
Planting Method ◽  
The One ◽  
And Staphylococcus Aureus

Isolation of marine fungi symbiont of green algae Bornetella sp as a producer of antibacterial compounds has been carried out. This study aims to obtain symbiont fungi from green algae Bornetella sp which produces antibacterial compounds. The symbiont fungus was isolated using the direct planting method. Screening for the antibacterial activity of pure symbiont fungi isolates against Escheriscia coli and Staphylococcus aureus, and was carried out by the agar diffusion method. The isolation results obtained 4 isolates, but the one with the most potential to inhibit the growth of the tested bacteria was MFALM2. Molecular characterization showed that the MFALM2 isolate was identified as Aspergillus nomius with a 100% closeness level. Keywords: isolation; marine fungi; Aspergillus nomius; green alga; Bornetella sp. AbstrakIsolasi jamur laut yang bersimbion dengan alga hijau Bornetella sp sebagai penghasil senyawa antibakteri telah dilakukan. Penelitian ini bertujuan untuk memperoleh jamur simbion dari alga hijau Bornetella sp yang menghasilkan senyawa antibakteri. Isolasi jamur simbion dilakukan dengan metode direct planting. Skrining aktivitas antibakteri isolat murni jamur simbion terhadap bakteri uji Escheriscia coli dan Staphylococcus aureus dilakukan dengan metode difusi agar.  Hasil isolasi diperoleh 4 isolat, namun yang paling berpotensi menghambat pertumbuhan bakteri uji adalah MFALM2. Karakterisasi molekuler menunjukkan bahwa isolat MFALM2 teridentifikasi sebagai Aspergillus nomius dengan tingkat keeratan sebesar 100%.Kata kunci: isolasi; jamur lau; Aspergillus nomius; alga hijau; Bornetella sp.

scholarly journals The Antibacterial Properties of Bayur Tissues’ Extract (Pterospermum subpeltatum C.B. Rob)

2014 ◽  
Vol 69 (5) ◽  
Author(s):  
Pince Salempa ◽  
Alfian Noor ◽  
Tjodi Harlim ◽  
Nunuk Hariani ◽  
Muharram Muharram ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Traditional Medicine ◽  
Secondary Metabolite ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Shigella Boydii ◽  
And Staphylococcus Aureus ◽  
Antibacterial Potential

Pterospermum is of plant genus in the Sterculiaceae family, which has never been studied for its secondary metabolite compound. The others species from this genus have long been used in generations of traditional medicine. For example, P. Javanicum has been used to treat dysentery, toothaches and ulcers. From the antibacterial testing carried out on some tissues of Pterospermum subpeltatum by GNA diffusion method,  this plant showed antibacterial potential, especially toward Shigella boydii and Staphylococcus aureus.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TANAMAN JOHAR (Cassia siamea Lamk.) DARI BEBERAPA TINGKAT KEPOLARAN PELARUT

KOVALEN ◽  
2017 ◽  
Vol 3 (3) ◽  
pp. 242
Author(s):  
Fitriah Fitriah ◽  
Mappiratu Mappiratu ◽  
Prismawiryanti Prismawiryanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Micrococcus Luteus ◽  
Solvent Polarity ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Antibacterial Compounds ◽  
Cassia Siamea ◽  
Activity Test

A research about antibacterial activity test of johar plant leaf extract (Cassia siamea Lamk.) using several levels of solvent polarity has been implemented. This study aims to determine whether the antibacterial compounds in the leaves is polar, non-polar or semi-polar, and to determine the highest inhibition level of johar leaf extract on both the gram-negative bacteria (Escherichia coli and Shigella dysenteriae) and gram-positive (Staphylococcus aureus and Micrococcus luteus). The maceration method was used in this research with 3 levels of solvent polarity namely non-polar, semi-polar and polar. The bacterial activity test was done with diffusion method. The results showed that the johar leaf contains antibacterial compounds which are polar and semi-polar. The highest inhibition level was obtained at johar leaf ethanol extract for Staphylococcus aureus, Escherichia coli, Micrococcus luteu and Shigella dysentriae of 14.9, 12.9, 12, and 7.2 mm, respectively.Keywords: Antibacterial, Inhibition Level, Johar Leaf Extract

scholarly journals Antibacterial activity of different extract of medicinal plant Antirrihnum Orontium.

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Shehzad Saleem ◽  
Wisal Ahmad ◽  
Muhammad Hanif ◽  
Zahid Ali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Water Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Water Extracts ◽  
The Family ◽  
Quaternary Base ◽  
And Staphylococcus Aureus

Antirrhinum Orontium is belonging to the family Plantaginacea commonly known as lesser snapdragon which is a flowering plant belong to genus Antirrhinum. It was traditionally used in treatment of Tumor, Eye inflammation and Ulcer. Antirrhinum Orontium is contain mostly tertiary alkaloids bases one of them is identified as 4-methyl-2, 6-naphthyridine and Choline is identified as quaternary base. The recent study is to investigate the antibacterial activity of Ethyl acetate, Dichloromethane, Hexane and Water extracts of Antirrhinum Orontium, against the pathogen like Escherichia coli and Staphylococcus aureus. Both the Soxhlet and Maceration water extract were found most active from the other extract against Escherichia coli and Staphylococcus aureus by using agar well diffusion method. The measured zone of inhibition for Soxhlet water extract is 21±0.05mm and Maceration water extract is 20±0.05mm against gram positive Staphylococcus aureus, while for gram negative Escherichia coli 23±0.05mm is measured for Soxlet and 21±0.05mm measured for Maceration water extracts

scholarly journals PENGARUH PEMANASAN, TINGKAT KEASAMAN (pH) DAN KONSENTRASI GARAM TERHADAP STABILITAS ANTI BAKTERI EKSTRAK ETANOL KULIT KAYU AKWAY (Drimys piperita Hook f.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba ◽  
Nitia Nilawati ◽  
Eka Syartika
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Heating Time ◽  
Diffusion Method ◽  
Ethanol Extracts ◽  
And Staphylococcus Aureus ◽  
Agar Well Diffusion Method

ABSTRACT  Akway (Drimys piperita) is a woody, evergreen and aromatic plant that belongs to family winteraceae. This plant is used by Sougb tribe lived in Sururey village, District of Anggi, to enhance the vitality of body. The objectives of the research were to determine antimicrobial stability of akway bark extracts influenced by heating time of 100OC, levels of acidity (pH) and salt contents.. Antimicrobial assays were done by using agar well diffusion method against four species of bacteria, i.e.  Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. The results showed that ethanolic extracts of akway bark only inhibited growth of B. cereus and S. aureus with minimum inhibitory concentration 0,99% and 0,89% . The levels of concentration and acidity of ethanol extracts  influenced the antimicrobial capacity of extracts.. Whereas heating time on 100OC during 25 minutes and salt contents  up to 5% of extract solution did not influence the antimicrobial stability of  akway bark extracts. Key words : akway, extracts, antimicrobe, pH, Heating, salt ABSTRAK Akway (Drimys piperita) adalah tumbuhan berkayu, aromatik dan hijau sepanjang tahun dan tergolong dalam suku winteraceae. Tumbuhan ini digunakan oleh Suku Sougb yang bermukim di desa Sururey Distrik Anggi, untuk mengobati malaria dan meningkatkan vitalitas tubuh. Tujuan dari penelitian ini adalah untuk menentukan stabilitas antimikroba ekstrak kulit kayu akway pada waktu pemanasan ekstrak pada 100OC, tingkat keasaman (pH) dan kandungan garam. Pengujian antimikroba dilakukan dengan menggunakan metode difusi agar terhadap empat spesies bakteri yaitu Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. Hasil menunjukkan bahwa ekstrak etanol kulit kayu akway hanya dapat menghambat bakteri Bacillus cereus dan Staphylococcus aureus dengan konsentrasi hambat minimum masing-masing adalah 0,99% dan 0,89%. Tingkat konsentrasi dan keasaman (pH) mempengaruhi kapasitas antimikroba ekstrak etanol kulit kayu akway. Sedangkan perlakuan pemanasan pada suhu 100OC dengan lama pemanasan sampai dengan 25 menit dan penambahan garam NaCl sampai konsentrasi 5%  tidak berpengaruh pada stabilitas antimikroba ekstrak etanol kulit kayu akway.  

scholarly journals Combination Gel Formulation Extracts of Moringa Leaf and Red Betel Leaf as an Inhibitor of Acne-Causing Bacteria (Propionibacterium acne and Staphylococcus aureus)

2021 ◽  
Vol 8 (3) ◽  
pp. 18-23
Author(s):  
Eko Sri Wahyuningsih ◽  
Wahono Sumaryono ◽  
Chaidir Chaidir
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Chemical Parameters ◽  
Physical And Chemical Parameters ◽  
Gel Preparation ◽  
Physical And Chemical ◽  
And Storage ◽  
And Staphylococcus Aureus

The most common skin disorder is acne (Acne vulgaris) which is a chronic inflammatory disease that occurs in the pilosebaceous unit. Infection can be caused by Propionibacterium acnes and Staphylococcus aureus. Moringa leaves are known to inhibit acne growth. Piper crocatum leaves are also thought to have antibacterial activity. The study aims to determine the effect of the combination of the two extracts in inhibiting the growth of acne-causing bacteria, also tested the gel formula to meet physical and chemical parameters as well as stability to temperature and storage, also to test the acute dermal irritation against rabbit. Each extract with a concentration of 0.3%; 0.6%; 1.25%; 2.5%; 5%; 10%; 20%, incubated then measured the inhibition zone, then determined the minimum inhibition zone concentration of the two extracts to determine the lowest levels of the extracts which still gave antibacterial activity against the tested bacteria by the well diffusion method. The extract combination was formulated in a gel preparation with the excipients Carbopol 940, PG, Phenoxyethanol, TEA, CMC, and Aquadest. Antibacterial activity test for gel preparations used the well method with positive control Medi-Klin® gel. Then the formula evaluation includes physical, chemical, and microbiological evaluations. The results showed that the combination gel form had activity against P. acne and S. aureus at concentrations of 2.5% : 2.5%. The combination gel preparation of the two extracts also has synergistic effect in inhibiting acne bacteria. The formula can meet physical and chemical parameters and is stable to temperature and storage.

scholarly journals ANTIBACTERIAL ACTIVITY OF Samanea saman LEAF ETHANOL EXTRACT AGAINST Escherichia coli AND Staphylococcus aureus AND ITS TOTAL FLAVONOID AND PHENOLIC CONTENTS

Jurnal Kimia ◽  
2018 ◽  
pp. 121
Author(s):  
W. S. Rita ◽  
I M. D. Swantara ◽  
I. A. R. Astiti Asih ◽  
N.K. Sinarsih
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
E Coli ◽  
Phenolic Contents ◽  
Agar Disc ◽  
Samanea Saman ◽  
And Staphylococcus Aureus

Antibacterial activity of Samanea saman usually shows a positif correlation to the flavonoid and phenolic contents.  The aim of this study was to evaluate the antibacterial activity of the ethanol extract of Samanea saman against Escherechia coli and Staphylococcus aureus and determine the total flavonoid and phenolic contents of the extract. The extraction was done by ethanol 96% at room temperature. The antibacterial assay was conducted by agar disc diffusion method. The total flavonoid and phenolic contents were determined by UV-Vis Spectrofotometer with the standard of quersetin and galic acid, respectively. The extraction of 250 g of Samanea saman leaves resulted in 24.5 g of ethanol extracts. The ethanol extract showed a moderate inhibition of 8.33 mm towards E. coli and a strong inhibition of 13.6 mm towards S. aureus at the concentration of 4%. The Minimum Inhibitory Concentration (MIC) of the extract against E. coli and S.aureus were of 3% and 0.3%”, respectively. The total flavonoid and phenolic contents were successively 1233.2991 mg QE/100g and 2544.6154 mg GAE/100g. Keywords: Escherechia coli, flavonoid and phenolic content,  Samanea saman, Staphylococcus aureus

Microwave Assisted Synthesis and Antibacterial Evaluation of 1, 3, 4-Thiadiazole Derivatives

2021 ◽  
pp. 5293-5296
Author(s):  
Jayalakshmi P M ◽  
Sheeba Jasmin TS ◽  
Manu Jose
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
New Drugs ◽  
Diffusion Method ◽  
Microwave Assisted Synthesis ◽  
Ir And Nmr Spectroscopy ◽  
Analgesic Agents ◽  
Ft Ir ◽  
And Staphylococcus Aureus ◽  
Heterocyclic Moiety

1,3,4-Thiadiazole is an important heterocyclic moiety, forms an integral core structural component of different categories of drugs such as antimicrobial, antitubercular, anti-inflammatory, antiepileptic, antiviral, antineoplastics, and analgesic agents. It is a key moiety in current discovery and designing of new drugs. The compounds were synthesised by both conventional method and microwave method. The targeted derivatives can be synthesised in a shorter time under microwave condition than under conventional reaction condition. Their structures were confirmed by FT-IR and NMR Spectroscopy. Antibacterial property of two synthesised analogs were evaluated by Agar well diffusion method against Escherichia coli and Staphylococcus aureus. The results of antibacterial activity showed that both the compounds were active against Staphylococcus aureus and inactive against Escherichia coli. Results of invitro studies showed that modifications in SB-2-PHB and SB-8-PHB will make it as a promising lead molecule for further research.

Hemolysis, Platelet Aggregation and Antibacterial Activities of Human Antiphospholipid Antibody

2020 ◽  
Vol 18 (3) ◽  
pp. 268-274
Author(s):  
Farzaneh Ahmadi Shapoorabadi ◽  
Maryam Sadat Mirbagheri Firoozabad ◽  
Neda Habibi ◽  
Giti Emtiazi
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Transmission Electron Microscopy ◽  
Antiphospholipid Antibodies ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Antiphospholipid Antibody ◽  
Bacterial Membranes ◽  
Transmission Electron ◽  
And Staphylococcus Aureus

Background: Anti-phospholipid antibodies have the potential to become an alternative to conventional antibiotics for humans. The Antiphospholipid Syndrome (APS) is an autoimmune disease where the body’s defense system incorrectly reacts against its own phospholipids. APS is distinct through the existence of venous and arterial thromboses, frequently multiple and recurring fetal losses, commonly accompanied by moderate thrombocytopenia. Anti-phospholipid antibodies include lupus anti-coagulant, anti- cardiolipin, anti-beta 2 glycoprotein 1, and anti-prothrombin antibodies. Methods: In this study, the mechanism of action of Anti-phospholipid antibodies against Klebsiella pneumonia and Staphylococcus aureus was investigated in great detail using a unique combination of imaging and biophysical techniques. Antibacterial activity of antiphospholipid antibodies was detected by a diffusion method and the investigation of the complexity of antibody-antigen was done by spectroscopic examination, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) imaging. Results: There was a profound change in the bacteria treated with healthy and patient serum in the optical microscopic study. In all of the studied fields, bacterial treatment with patient serum immediately induced bacterial swelling and cumulative accumulation of the bacteria while no changes were observed in the healthy serum. Anti-bacterial activities of patient serum were detected on the plate. The result of this study showed that after platelet activation by thrombin and incubation with antiphospholipid antibodies, the platelet was aggregated. The transmission electron microscopy (TEM) image showed that the cell wall of Klebsiella pneumonia and Staphylococcus aureus incubated with antiphospholipid had a bizarre shape and antiphospholipid antibodies bound to bacterial membranes. Conclusion: The data indicated that antiphospholipid antibodies with hemolysis activities have an effect on Gram-positive and negative bacteria and these antibodies have the potential to become antibiotic for human.

scholarly journals Antibacterial Action and Efficacy of Silver and Copper Exchanged Na-Y Zeolite from Fly Ash on 304 Stainless Steel Coupons by Agar Diffusion Method

2021 ◽  
Vol 33 (2) ◽  
pp. 371-375
Author(s):  
Rashmi Rani Padhy ◽  
Sangeeta Tiwari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Fly Ash ◽  
Zeolite Y ◽  
Exchange Process ◽  
304 Stainless Steel ◽  
Antibacterial Action ◽  
Diffusion Method ◽  
Y Zeolite ◽  
And Staphylococcus Aureus

Na-Y Zeolite synthesized from fly ash was exchanged with Ag+ and Cu2+ ions by ion exchange process. The antibacterial action and efficacy of silver and copper exchanged Na-Y zeolite on Escherichia coli and Staphylococcus aureus were investigated. Escherichia coli and Staphylococcus aureus was suspended in broth media and exposed to silver and copper exchanged zeolite Y (ranging from 25-1000 μg) for a period of 24 h. The minimum bactericidal concentration (MBC) of silver and copper loaded zeolite-Y powder was 50 and 250 μg for Escherichia coli. Staphylococcus aureus was destroyed at a powder concentration of 100 μg for both Ag-Y and Cu-Y zeolite. Further, the present work also focuses to incorporate 5% silver and copper exchanged zeolite pigment in coating and study the antibacterial effect on the coated 304 stainless coupons. Paint containing copper exchanged zeolite shows antibacterial activity compared to paint with silver containing zeolite.

scholarly journals ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF MANGIFERA INDICA ETHANOL AND AQUEOUS LEAVES EXTRACT AGAINST PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 8 (12) ◽  
pp. 262-269
Author(s):  
M. A. Garga ◽  
U. M. Garasin ◽  
M. Abdullahi ◽  
B. A. Muhammed ◽  
A. Yakubu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Gram Staining ◽  
Significant Difference ◽  
Zones Of Inhibition ◽  
And Staphylococcus Aureus

The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.

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