scholarly journals Laxative Activity Test of Gynura procumbens (Lour.) Merr. Leave on White Male Mice Induced by Loperamide

2019 ◽  
Vol 6 (2) ◽  
pp. 137
Author(s):  
Rahimatul Uthia ◽  
Devina Prima Yolanda ◽  
Aried Eriadi ◽  
Dwi Dinni Aulia Bakhtra
Keyword(s):  
White Male ◽  
Ethanol Extract ◽  
Negative Control ◽  
Intestinal Wall ◽  
Male Mice ◽  
Direct Stimulation ◽  
Activity Test ◽  
Intestinal Peristalsis ◽  
Ethanol Extracts ◽  
Stimulation Of

Constipation is a symptom of a problematic, non-smooth and irregular defecation process. While laxative is a substance that stimulates intestinal peristalsis as a reflex of direct stimulation of the intestinal wall that can cause or facilitate defecation. This study aimed to determine the ethanol extract of the leaves of Gynura procumbens (L.) Merr. effect as a laxative in white male mice induced by loperamide. The methods used for laxative testing are defecation patterns and intestinal transit methods. The parameters observed included frequency of defecation, stool consistency, stool weight every 30 minutes for 4 hours, and the length of the activated carbon in the mice's intestine. The extract was administered orally at a dose of 75 mg/kg BW, 150 mg/kg BW and 300 mg/kg BW, as a comparison used negative control (Na CMC). From the results of the study, it was found that the administration of ethanol extracts of Gynura procumbens (Lour.) Merr. leaves at the dose of 150 mg/kg BW gave laxative activity to white male mice (P <0.05).

scholarly journals Utilizing of 96% Etanol Extract Activity Red Leaf (Syzygium myrtifolium Walp.) Maseration and Socletation Method as an Antidiarrhea at Male Mice

2019 ◽  
Vol 8 (2S7) ◽  
pp. 260-263
Keyword(s):  
Effective Dose ◽  
Ethanol Extract ◽  
Extraction Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Traditional Medicines ◽  
Intestinal Length ◽  
Child Morbidity ◽  
Ethanol Extracts

Diarrhea is still one of the most important public health problems because it is the third major contributor to child morbidity and mortality in Indonesia. One of the traditional medicines that can be used to treat diarrhea is red shoots. Red shoot leaves contain secondary metabolites such as alkaloids, flavonoids, chalcones, tannins, and terpenoids which can be used as antidiarrheals. The purpose of this study was to compare the antidiare effects between extracts of maceration and socletation of red shoot leaves and determine the effective dose of both extracts as antidiarrheal in male mice using the intestinal transit method. The treatment in this study consisted of the administration of ethanol extract of shoots of red shoots by maceration at a dose of 1.58 mg / 20 g BW (dose of MI), 4.75 mg / 20g BW (dose of M.II), 6.33 mg / 20g BB (Dosage M.III) and ethanol extract of shoots of red shoots by socletation at a dose of 1.67 mg / 20 g BB (SI dose), 5.04 mg / 20 g BB (Dosage S.II), 6.72 mg / 20 g BB (Dose S.III), Loperamid HCl as positive control, and 1% CMC suspension as negative control. Observations were made by measuring the ratio of intestinal length traveled through the norit (marker) to the total gut length. The results showed that all dose treatments on maceration and socletation ethanol extracts had antidiareal activity. Different extraction methods give the same effect in giving antidiarrheal effects. Based on Duncan's advanced dose of 6.72 mg / 20 g BW of socletation results is an effective dose as antidiarrheal because it has an effect that is not significantly different than Loperamid HCl.

scholarly journals Hydrogel Activity Test from Kirinyu Leaf Ethanol Extract (Chromolaenaodorata (L.) R. King & H. Rob) and Combination of Kirinyu Leaf Ethanol Extract with Collagen As A Healing Wound Excision.

2020 ◽  
Vol 8 (1) ◽  
pp. 35-37
Author(s):  
Thursina C S ◽  
Julia R ◽  
Sumaiyah S
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Activity Test ◽  
Collagen Group ◽  
Healing Wound ◽  
Wound Excision ◽  
Group 2 ◽  
Ethanol Extracts ◽  
Group 1

Objective: the purpose of this study was to determine the activity of the kirinyu leaf ethanol extract hydrogel preparation and the combination of the kirinyu leaf ethanol extract with collagen as a healing wound excision. Method: This research is experimental, where the ethanol extracts of Kirinyu leaves and collagen are formulated in the form of hydrogel preparations and tested on the healing of excision wounds in rats. Testing wound healing activities were divided into 6 groups. The Group 1 (negative control) were given hydrogel base without Kirinyu leaf ethanol extract in combination with collagen, group 2 (positive control) were given Octenilin®gel, groups 3 and 4, were given hydrogel of Kirinyu leaf ethanol extract while groups 5 and 6 were given hydrogel extract of kirinyu leaves combined with collagen. Result: The results of percent reduction in wound diameter showed that groups 3 and 4 gave significant differences compared to group 1 which began to be seen on the 10th day (p <0.05). While groups 5 and 6 gave significant differences that began to be seen on the 8th day (p <0.05). Conclusion:Hydrogel preparation in group 6 has the most potential in healing excision wounds.    

scholarly journals Ethanolic Extract Oitment of The Graptophylum Pictum L Griff Leaves as Analgesic agent to Male White Rat

2020 ◽  
Vol 3 (1) ◽  
pp. 32
Author(s):  
Setya Enti Rikomah ◽  
Ninsi Fitri
Keyword(s):  
White Male ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Good Effect ◽  
Test Results ◽  
Male Mice ◽  
Average Percentage ◽  
One Way Anova

<p>Graptophyllum pictum L Griff Leaves in the community are used as medicinal ingredients, one of which is for analgesics. Black pudding leaves contain secondary metabolite compounds that act as suspected analgesics, namely flavonoids. The ethanolic extract of black pudding leaves has been formulated into cream and ointment preparations. The purpose of this study was to determine the effectiveness of analgesic preparations of black pudding leaves (Graptophyllum pictum L. Griff) ethanol extract in white male mice. The effectiveness of analgesics was tested using the hotplate method. The test animals used were 25 white male mice which were divided into 5 groups: negative control (F0), positive control (Ichtiol® ointment), and black pudding leaf F1 (10%), F2 (15%) ethanol extract treatment group, and F3 (20%). Observation of stretching of mice was done after the feet of mice were induced and immediately given treatment. Observation of analgesic percentage in each mouse was carried out every 5 minutes for 60 minutes. The data obtained were processed and analyzed using one-way ANOVA and Duncan test with a 95% confidence level. One-way ANOVA test results showed that there were significant differences between treatment groups (0.003 &lt;0.05). The results of the average percentage of analgesic effectiveness of formula 2 have a good effect that is equal to 77.969%. Duncan's test results showed that the group that gave the best analgesic effectiveness was formula 2 (15%) comparable to positive control.</p><p><strong>Keywords: Graptophyllum pictum,  Analgesics, ointment, Hotplate.</strong><strong></strong></p>

Analgesic Effectivty Of Nanoparticles Chitosan-Ethanol Leaf Extract Papaya (Carica Papaya L.) In White Male Mice (Mus Mucculus)

2017 ◽  
Vol 15 (2) ◽  
pp. 228
Author(s):  
Juliferd Gredi ◽  
Wintari Taurina ◽  
Mohamad Andrie
Keyword(s):  
Leaf Extract ◽  
Carica Papaya ◽  
White Male ◽  
Ethanol Extract ◽  
Test Method ◽  
Ionic Gelation ◽  
Male Mice ◽  
Writhing Test ◽  
Effi Ciency ◽  
Optimal Formula

Papaya leaves has been shown to have efficacy as an analgesic. Analgesic effect caused by the flavonoids in leaves of papaya. To enhance the analgesic effi cacy papaya into dosage formulatio of nanoparticles. The purpose of this research was to create and characterized nanoparticles from ethanol extract of papaya leaves then tested for effectiveness as an analgesic. This study used polymer chitosan and NaTPP to created nanoparticles through ionic gelation method and analgesic activity tested using writhing test method and statistical tested. Optimal formula of nanoparticles were produced at concentration of 0.2% chitosan, Na-TPP extract 0.1% and 4% with a ratio of 6: 1: 1. The nanoparticles was formed a size of 255.4 nm with polidispers index of 0.7, the zeta potential of + 11,1mV, the adsorption effi ciency by 87% and rounded morphology (spherical). Analgesic effectiveness tested by comparison between the groups ethanol extract of papaya and the groups nanoparticles of chitosan-ethanol extract papaya statistically using the T-test Independent. The results of this research indicate that there are statistically signifi cant differences (p <0.05). This results shows that the preparation nanoparticles of chitosan-ethanol extract papaya is more effective as an analgesic than the ethanol extract of papaya leaves.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals FORMULATION, STABILTY TEST AND ENZYME ACTIVITY TEST TYROSINASE OF CREAM PEPARATIONS FROM HERENDONG FRUIT EXTRACT(Melastoma affine D. Don)

2020 ◽  
Vol 5 (2) ◽  
pp. 149-173
Author(s):  
Zuraida Sagala ◽  
Kurnia Telaumbanua
Keyword(s):  
Enzyme Inhibitors ◽  
Enzyme Inhibitor ◽  
Mechanical Test ◽  
Ethanol Extract ◽  
Activity Test ◽  
Irritation Test ◽  
Ic50 Values ◽  
Tyrosinase Enzyme ◽  
Ethanol Extracts ◽  
The Stability

One way to prevent or inhibit melanin formation is by inhibiting tyrosinase activity (Lloyd,2011). Tyrosinase enzyme is an enzyme that plays a role in the formation of skin pigments or known as melanogenesis. In the process of melanogenesis, the enzyme tyrosinase acts as a catalyst in two different reactions, namely the hydroxylation process of tyrosine to dihydroxy-phenylalanine (L-DOPA) and oxidation of L-DOPA to quinone DOPA. Tyrosinase in skin tissue is activated by solar UV radiation so that it accelerates the process of melanin production (Fais et al.  2009).  This  study  was  conducted  to  determine  the  stability,  effectiveness  of  cream preparations from ethanol extracts of Harendong fruit (Melastoma affine D. Don) as tyrosinase enzyme inhibitors so it can be used as a cosmetic ingredient for whitening or skin lightening. Results Cream with variants of Harendong (Melastoma affine D. Don) ethanol extract was physically stable and formulated with organoleptic test, homogeneity, viscosity, mechanical test, pH, cycling test, and irritation test. Type M / A cream preparations from ethanol extract of Harendong fruit (Melastoma affine D. Don) have a strong / moderate tyrosinase enzyme inhibitor activity with IC50 values of F1 (1%) of 526,192 ppm, F2 (1.5%) ) amounted to 317,534 ppm and F3  (2%)  amounted  to  128,523  ppm.  Keywords: Foemulation,  stability test, activity test, tyrosinase enzyme, herenong fruit (Melastoma affine G.Don)

scholarly journals Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)

2017 ◽  
Vol 6 (2) ◽  
pp. 125
Author(s):  
Rizkayanti Rizkayanti ◽  
Anang Wahid M. Diah ◽  
Minarni Rama Jura
Keyword(s):  
Vitamin C ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ic50 Value ◽  
Antioxidant Potency ◽  
Ethanol Extracts ◽  
Moringa Oleifera Lam

Moringa (moringa oleifera Lam) leaves contains many molecules as inhibitors for free radicals such as phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, tannins), nitrogen compounds (alkaloids, amines, betalain), vitamins, terpenoids (including carotenoids), and several other endogenous metabolites as antioxidants. This study aimed to determine the antioxidant potency of water and ethanol extracts of moringa (moringa oleifera Lam) leave obtained by maceration and dekok. The concentration of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was analyzed using UV-Vis spectrophotometer after addition of various concentrations of Moringa leaves extracts. Various concentrations of moringa leave extracts used were 20 ppm, 40 ppm, 60 ppm and 80 ppm. Vitamin C solutions as the positive control were prepared on similar various concentrations. The negative control was prepared using DPPH solutions dissolved in absolute ethanol. The results indicated that the ethanol extract of moringa leaves prepared by maceration method showed the antioxidant potency with an IC50 value of 22.1818 ppm, but the IC50 value of water extract of moringa leaves prepared by dekok was 57.5439 ppm. While, the IC50 value of Vitamin C was 8.8084 ppm. Based on the IC50 data it can be concluded that Vitamin C is a stronger antioxidant than moringa leaves extracts.

scholarly journals AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL 70% DAUN ASHITABA (Angelica keiskei) SECARA IN VIVO DENGAN PENGINDUKSI KARAGENAN

2019 ◽  
Author(s):  
Haka As'ada ◽  
Yardi Saibi ◽  
Hendri Aldrat
Keyword(s):  
Dose Range ◽  
White Male ◽  
Ethanol Extract ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Male Rat ◽  
Control Group ◽  
Angelica Keiskei ◽  
Anti Inflammatory

Ashitaba leaves (Angelica keiskei) or also known as tommorow's leaf is plant that known to have various health benefit, one of them is as an anti-inflammatory activity. The anti-inflammatory activity of ashitaba leaves has been known through in vitro assays. This study aims to determine the anti-inflammatory activity of 70% ethanol extract of ashitaba leaves through in vivo assay. Anti-inflammatory activity was performed on white male rat of Sprague dawley strain with induction method of edema on rat's foot using 1% carrageenan 0.2 ml. Rats were divided into 5 groups. The negative control group was given a 0.5% Na-CMC suspension, a positive control group was given sodium diclofenac suspension of 5.14 mg / kgBW, and the test group was given 70% ethanol extract of ashitaba leaves at a dose of 1000; 2000; and 4000 mg / kgBW suspended in 0.5% Na-CMC. The results showed that in that dose range the 70% ethanol extract of ashitaba leaves had anti-inflammatory activity that did not depend on the dose. Percentage of edema of 70% ethanol extract of ashitaba leaves dose 1000; 2000; 4000 mg / kgBB was significantly different with negative control (p ≤ 0,05) and had percentage of edema inhibition respectively 83,95%, 79,01%, and 80,25%. The results of this study showed that 70% ethanol extract of ashitaba leaves have anti-inflammatory activity. Keywords: Ashitaba, Angelica keiskei, tommorow's leaf, anti-inflammatory, carrageenan.

Aktivitas Antibakteri dari Ekstrak Etanol Ganoderma sp. Asal Pulau Lombok

2020 ◽  
Vol 6 (1) ◽  
pp. 22
Author(s):  
Adelina Oktaviani ◽  
Aida Muspiah ◽  
Faturrahman Faturrahman
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Activity ◽  
Large Diameter ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Gram Negative Bacteria ◽  
Activity Test

The use of antibiotics that are not according to the rules and antibiotics in the long term can cause resistance to bacteria. This study aims to determine the presence of antibacterial activity and the effect of increasing the concentration of ethanol extract of Ganoderma sp. against several test bacteria. Extract from Ganoderma sp. obtained by maceration method using ethanol 95% solvent. The extract concentrations used were 20%, 40%, 60% and 80%. This research was conducted using the wells method with ciprofloxacin as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition formed around the well. The results of the antibacterial activity test of ethanol extract Ganoderma sp. has greater inhibitory activity against gram-negative bacteria. The inhibitory activity of ethanolic extract of Ganoderma sp. on the growth of test bacteria increased with increasing concentration of the extract

scholarly journals Stimulation of both estrogen and androgen receptors maintains skeletal muscle mass in gonadectomized male mice but mainly via different pathways

2010 ◽  
Vol 45 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Johan Svensson ◽  
Sofia Movérare-Skrtic ◽  
Sara Windahl ◽  
Charlotte Swanson ◽  
Klara Sjögren
Keyword(s):  
Skeletal Muscle ◽  
Muscle Mass ◽  
Skeletal Muscle Mass ◽  
Skeletal Muscle Atrophy ◽  
Gene Transcript ◽  
Male Mice ◽  
Direct Stimulation ◽  
Muscle Weight ◽  
Peripheral Tissues ◽  
Stimulation Of

Testosterone is a major regulator of muscle mass. Little is known whether this is due to a direct stimulation of the androgen receptor (AR) or mediated by aromatization of testosterone to estradiol (E2), the ligand for the estrogen receptors (ERs), in peripheral tissues. In this study, we differentiated between the effects mediated by AR and ER by treating orchidectomized (orx) male mice for 5 weeks with E2 or the non-aromatizable androgen dihydrotestosterone (DHT). Both E2 and DHT increased muscle weight and lean mass, although the effect was less marked after E2 treatment. Studies of underlying mechanisms were performed using gene transcript profiling (microarray and real-time PCR) in skeletal muscle, and they demonstrated that E2 regulated 51 genes and DHT regulated 187 genes, with 13 genes (=25% of E2-regulated genes) being regulated by both treatments. Both E2 and DHT altered the expression of Fbxo32, a gene involved in skeletal muscle atrophy, affected the IGF1 system, and regulated genes involved in angiogenesis and the glutathione metabolic process. Only E2 affected genes that regulate intermediary glucose and lipid metabolism, and only DHT increased the expression of genes involved in synaptic transmission and heme and polyamine biosynthesis. In summary, ER activation by E2 treatment maintains skeletal muscle mass after orx. This effect is less marked than that of AR activation by DHT treatment, which completely prevented the effect of orx on muscle mass and was partly, but not fully, mediated via alternative pathways.

Sign in / Sign up

Export Citation Format

Share Document