LC-MS TECHNIQUE & ITS METABOLOMIC BASED APPROACHES IN SCIENCE AND TECHNOLOGY

2021 ◽  
pp. 211-216
Author(s):  
Juhi U. Bandre ◽  
Puja R. Basule ◽  
Atul T. Hemke ◽  
Milind J.Umekar
Keyword(s):  
Analytical Techniques ◽  
Low Molecular Weight ◽  
Analytical Technique ◽  
Cellular Processes ◽  
Cell Tissue ◽  
Target Analytes ◽  
Different Types ◽  
Complete Set ◽  
Endogenous Metabolites ◽  
Good Coverage

The metabolome is the complete set of metabolites found during a biological cell, tissue, organ or organism, representing the end product of cellular processes. Metabolomics is rising platform that enables one to research global endogenous metabolites with low molecular weight in biological systems. On the other hand, an acceptance of various analytical technique is predicated on procedures with successfully carried out for completely different types of metabolites on which Liquid chromatography (LC) in combination with tandem mass spectrometry (MS/MS) has enjoyed a growing quality. LC-MS is the best platform for metabolomic studies because of its glorious sensitivity, resolution, high throughput, soft ionization, and good coverage of metabolites. Also, an ability that can accurately and precisely discriminate target analytes from high complexity mixtures in a sensitive and selective way. In this review article we have discussed an omics and its different types, mainly focused on metabolomics and various application of metabolomics using LC-MS analytical technique. Here we collected all information regarding applications of metabolomics known by LC-MS. Highlight necessary pit falls in the process of metabolomics using LC-MS techniques and address multiple advantages of implementing LC-MS technique over another analytical techniques for metabolomics during a varied approaches.

Recent Developments in CE-MS Based Metabolomics

2020 ◽  
Vol 16 ◽  
Author(s):  
Mustafa Çelebier ◽  
Merve Nenni
Keyword(s):  
Sample Preparation ◽  
Scientific Information ◽  
Analytical Techniques ◽  
Living Organism ◽  
Analytical Technique ◽  
Ionic Compounds ◽  
Recent Sample ◽  
Recent Developments ◽  
Endogenous Metabolites ◽  
Preparation Techniques

Background: Metabolomics has gained importance in clinical applications over the last decade. Metabolomics studies are significant because the systemic metabolome is directly affected by disease conditions. Metabolome-based biomarkers are actively being developed for early diagnosis and to indicate the stage of specific diseases. Additionally, understanding the effect of an intervention on a living organism at the molecular level is a crucial strategy for understanding novel or unexpected biological processes. Results: The simultaneous improvements in advanced analytical techniques, sample preparation techniques, computer technology, and databank contents has enabled more valuable scientific information to be gained from metabolomics than ever before. With over 15,000 known endogenous metabolites, there is no single analytical technique capable of analyzing the whole metabolome. However, capillary electrophoresis-mass spectrometry (CE-MS) is a unique technique used to analyze an important portion of metabolites not accessible by liquid chromatography or gas chromatography techniques. The analytical capability of CE, combined with recent sample preparation techniques focused on extracting polar-ionic compounds, make CE-MS a perfect technique for metabolomic studies. Conclusion: Here, previous reviews of CE-MS based metabolomics are evaluated to highlight recent improvements in this technique. Specifically, we review papers from the last two years (2018 and 2019) on CE-MS based metabolomics. The current situation and the challenges facing metabolomic studies are discussed to reveal the high potential of CE-MS for further studies, especially in biomarker development studies.

Integration of Immunochemical Methods with Other Analytical Techniques for Pesticide Residue Determination

1995 ◽  
Vol 78 (3) ◽  
pp. 585-591 ◽  
Author(s):  
Anne D Lucas ◽  
Shirley J Gee ◽  
Bruce D Hammock ◽  
James N Seiber
Keyword(s):  
Small Molecules ◽  
Analytical Techniques ◽  
Low Molecular Weight ◽  
Solvent Exchange ◽  
New Techniques ◽  
Analytical Technique ◽  
Residue Determination ◽  
Organic Sample ◽  
Sample Extract ◽  
The Matrix

Abstract The growing volume of literature concerning immunoassay analysis for trace levels of agrochemicals and other low molecular weight contaminants in various matrixes is indicative of the tremendous interest in and utility of this analytical technique. Most immunoassay methods described in the literature analyze compounds directly, for example, a herbicide in water, or involve solvent exchange of an organic sample extract or dilution of an aqueous-based sample to minimize the matrix effect. As immunoassay for small molecules becomes widely accepted and applied, new challenges involving more complex chemicals in more difficult matrixes arise. The integration of “classical” analytical chemistry with immunochemistry can provide new techniques and approaches useful in discovering the movement, mode of action, and ultimate impact of certain chemicals on humans and the environment.

Anticancer Properties of Essential Oils: An Overview

2018 ◽  
Vol 18 (10) ◽  
pp. 957-966 ◽  
Author(s):  
Milene Aparecida Andrade ◽  
Mariana Aparecida Braga ◽  
Pedro Henrique Souza Cesar ◽  
Marcus Vinicius Cardoso Trento ◽  
Mariana Araújo Espósito ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Essential Oils ◽  
Public Health Problem ◽  
Mechanisms Of Action ◽  
Low Molecular Weight ◽  
Anticancer Properties ◽  
Anti Cancer ◽  
Different Types ◽  
Antitumor Properties ◽  
Pharmaceutical Properties

Background: Essential oils are complex mixtures of low molecular weight compounds extracted from plants. Their main constituents are terpenes and phenylpropanoids, which are responsible for their biological and pharmaceutical properties, such as insecticidal, parasiticidal, antimicrobial, antioxidant, anti-inflammatory, analgesic, antinociceptive, anticarcinogenic, and antitumor properties. Cancer is a complex genetic disease considered as a serious public health problem worldwide, accounting for more than 8 million deaths annually. Objective: The activities of prevention and treatment of different types of cancer and the medicinal potential of essential oils are addressed in this review. Conclusion: Several studies have demonstrated anti-carcinogenic and antitumor activity for many essential oils obtained from various plant species. They may be used as a substitution to or in addition to conventional anti-cancer therapy. Although many studies report possible mechanisms of action for essential oils compounds, more studies are necessary in order to apply them safely and appropriately in cancer therapy.

scholarly journals The Role of High-Resolution Analytical Techniques in the Development of Functional Foods

2021 ◽  
Vol 22 (6) ◽  
pp. 3220
Author(s):  
Álvaro Fernández-Ochoa ◽  
Francisco Javier Leyva-Jiménez ◽  
María De la Luz Cádiz-Gurrea ◽  
Sandra Pimentel-Moral ◽  
Antonio Segura-Carretero
Keyword(s):  
High Resolution ◽  
Functional Foods ◽  
Intervention Studies ◽  
Analytical Techniques ◽  
Nutritional Intervention ◽  
Bioactive Properties ◽  
Metabolic Reactions ◽  
Bioactive Potential ◽  
Endogenous Metabolites

The approaches based on high-resolution analytical techniques, such as nuclear magnetic resonance or mass spectrometry coupled to chromatographic techniques, have a determining role in several of the stages necessary for the development of functional foods. The analyses of botanical extracts rich in bioactive compounds is one of the fundamental steps in order to identify and quantify their phytochemical composition. However, the compounds characterized in the extracts are not always responsible for the bioactive properties because they generally undergo metabolic reactions before reaching the therapeutic targets. For this reason, analytical techniques are also applied to analyze biological samples to know the bioavailability, pharmacokinetics and/or metabolism of the compounds ingested by animal or human models in nutritional intervention studies. In addition, these studies have also been applied to determine changes of endogenous metabolites caused by prolonged intake of compounds with bioactive potential. This review aims to describe the main types and modes of application of high-resolution analytical techniques in all these steps for functional food development.

Preface

2006 ◽  
Vol 78 (8) ◽  
pp. iv
Author(s):  
Richard J. Cogdell
Keyword(s):  
International Symposium ◽  
De Novo ◽  
Analytical Techniques ◽  
Carotenoid Biosynthesis ◽  
Annual Growth Rate ◽  
Chemical Company ◽  
Cellular Processes ◽  
Poster Sessions ◽  
14Th International Symposium ◽  
Oral Communications

The 14th International Symposium on Carotenoids was held in Edinburgh, Scotland, UK 17-22 July 2005, under the chairmanship of Dr. George Britton. The International Symposium on Carotenoids is the official symposium for the International Carotenoid Society (http://carotenoidsociety.org), which supported the symposium as did IUPAC. Financial support was gratefully received from DSM Nutritional Products, BASF Ag, Cognis Deutschland, Fuji Chemical Company Ltd., Inexa Industria Extractora CA, Valensa International, Nu Skin International Inc., Cargill Inc., The Alcon Foundation Inc., Kemin Health, Access Business Group, and LycoRed Natural Products Industries Ltd.The first International Symposium took place in Trondheim, Norway in 1966, and such meetings have continued at three-year intervals since then. Over that period of almost 40 years, the carotenoids field has expanded tremendously and diversified into many fields of study, especially human nutrition and health. There have also been continued advances in our understanding of the roles of carotenoids in photosynthesis and photochemistry, the regulation of their formation, de novo chemical synthesis, and the analytical techniques available for detailed structural analyses. The commercial importance of carotenoids has also significantly increased over the years; the current market was estimated to be around $887 million for 2004 and is expected to rise at an average annual growth rate of 2.9 % to just over $1 billion.These areas were fully reflected in the 220 invited lectures, oral communications, and poster sessions. The seven articles that appear in this issue embody the themes of the symposium, namely:- Carotenoids and Health: a series of themed sessions focusing on protection against disease, the eye, molecular and cellular processes, and nutrition- Carotenoid Oxidation and Breakdown Products and Metabolites- Carotenoids in Photosynthesis- Carotenoid Biosynthesis- Commercial Production and Applications- Carotenoids and Nature: ecology, etc.- Molecular Interactions of CarotenoidsFinally, we would like to thank everyone who contributed to a most successful symposium, including the local organizing committee, and look forward to the next meeting in 2008, which will be held in Okinawa, Japan and will be chaired by Prof. Hideki Hashimoto.Richard J. CogdellPeter M. BramleyConference Editors

scholarly journals Mycotoxin Analysis: New Proposals for Sample Treatment

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Natalia Arroyo-Manzanares ◽  
José F. Huertas-Pérez ◽  
Ana M. García-Campaña ◽  
Laura Gámiz-Gracia
Keyword(s):  
Raw Materials ◽  
Animal Health ◽  
Analytical Techniques ◽  
Sample Treatment ◽  
Analytical Technique ◽  
Chemical Structures ◽  
Technical Developments ◽  
Food And Feed ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Food Commodities

Mycotoxins are toxic secondary metabolites produced by different fungi, with different chemical structures. Mycotoxins contaminate food, feed, or raw materials used in their production and cause diseases and disorders in humans and livestock. Because of their great variety of toxic effects and their extreme heat resistance, the presence of mycotoxins in food and feed is considered a high risk to human and animal health. In order to ensure food quality and health consumers, European legislation has set maximum contents of some mycotoxins in different matrices. However, there are still some food commodities susceptible to fungal contamination, which were not contemplated in this legislation. In this context, we have developed new analytical techniques for the multiclass determination of mycotoxins in a great variety of food commodities (some of them scarcely studied), such as cereals, pseudocereals, cereal syrups, nuts, edible seeds, and botanicals. Considering the latest technical developments, ultrahigh performance liquid chromatography coupled to tandem mass spectrometry has been chosen as an efficient, fast, and selective powerful analytical technique. In addition, alternative sample treatments based on emerging methodologies, such as dispersive liquid-liquid microextraction and QuEChERS, have been developed, which allow an increased efficiency and sample throughput, as well as reducing contaminant waste.

scholarly journals The Role of Non-Coding RNAs in the Regulation of the Proto-Oncogene MYC in Different Types of Cancer

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 921
Author(s):  
Ekaterina Mikhailovna Stasevich ◽  
Matvey Mikhailovich Murashko ◽  
Lyudmila Sergeevna Zinevich ◽  
Denis Eriksonovich Demin ◽  
Anton Markovich Schwartz
Keyword(s):  
Malignant Tumors ◽  
Current Data ◽  
Cellular Processes ◽  
Cell Divisions ◽  
Specific Tumor ◽  
Different Types ◽  
Myc Gene ◽  
Non Coding Rnas ◽  
Tumor Types

Alterations in the expression level of the MYC gene are often found in the cells of various malignant tumors. Overexpressed MYC has been shown to stimulate the main processes of oncogenesis: uncontrolled growth, unlimited cell divisions, avoidance of apoptosis and immune response, changes in cellular metabolism, genomic instability, metastasis, and angiogenesis. Thus, controlling the expression of MYC is considered as an approach for targeted cancer treatment. Since c-Myc is also a crucial regulator of many cellular processes in healthy cells, it is necessary to find ways for selective regulation of MYC expression in tumor cells. Many recent studies have demonstrated that non-coding RNAs play an important role in the regulation of the transcription and translation of this gene and some RNAs directly interact with the c-Myc protein, affecting its stability. In this review, we summarize current data on the regulation of MYC by various non-coding RNAs that can potentially be targeted in specific tumor types.

scholarly journals Chirality, Gelation Ability and Crystal Structure: Together or Apart? Alkyl Phenyl Ethers of Glycerol as Simple LMWGs

Symmetry ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 732
Author(s):  
Alexander A. Bredikhin ◽  
Aidar T. Gubaidullin ◽  
Zemfira A. Bredikhina ◽  
Robert R. Fayzullin ◽  
Olga A. Lodochnikova
Keyword(s):  
Self Assembly ◽  
Chiral Recognition ◽  
Soft Materials ◽  
Gelling Agent ◽  
Low Molecular Weight ◽  
X Ray Diffraction ◽  
X Ray ◽  
Different Types ◽  
Low Molecular Weight Gelators ◽  
Phenyl Ethers

Chiral recognition plays an important role in the self-assembly of soft materials, in particular supramolecular organogels formed by low molecular weight gelators (LMWGs). Out of 14 pairs of the studied racemic and enantiopure samples of alkyl-substituted phenyl ethers of glycerol, only eight enantiopure diols form the stable gels in nonane. The formation of gels from solutions was studied by polarimetry, and their degradation with the formation of xerogels was studied by the PXRD method. The revealed crystalline characteristics of all studied xerogels corresponded to those for crystalline samples of the parent gelators. In addition to those previously investigated, crystalline samples of enantiopure para-n-alkylphenyl glycerol ethers [alkyl = pentyl (5), hexyl (6), heptyl (7), octyl (8), nonyl (9)] and racemic 3-(3,5-dimethylphenoxy)propane-1,2-diol (rac-14) have been examined by single crystal X-ray diffraction analysis. Among 22 samples of compounds 1-14 studied by SC-XRD, seven different types of supramolecular motifs are identified, of which only two are realized in crystals of supramolecular gelators. An attempt was made to relate the ability to gel formation with the characteristics of the supramolecular motif of a potential gelling agent, and the frequency of formation of the motif, required for gelation, with the chiral characteristics of the sample.

scholarly journals Commentary on Ivancic et al.: Enzyme kinetics from circular dichroism of insulin reveals mechanistic insights into the regulation of insulin-degrading enzyme

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Giuseppe Grasso
Keyword(s):  
Analytical Techniques ◽  
Label Free ◽  
Insulin Degrading Enzyme ◽  
Degrading Enzyme ◽  
Analytical Technique ◽  
Biochemical Pathways ◽  
New Methods ◽  
The World ◽  
Free Environment ◽  
Enormous Number

Despite the enormous number of therapeutic advances in medicine, nowadays many diseases are still incurable, mainly due to the lack of knowledge of the pathological biochemical pathways triggering those diseases. For this reason, it is compulsory for the scientific community to investigate and unveil the biomolecular mechanisms responsible for the development of those diseases, such as Alzheimer’s disease and diabetes, which are widespread all over the world. In this scenario, it is of paramount importance to develop new analytical techniques and experimental procedures that are capable to make the above-mentioned investigations feasible. These new methods should allow easy performable analysis carried out in a label-free environment, in order to give reliable answers to specific biochemical questions. A recent paper published on Bioscience Reports by Ivancic et al. (https://doi.org/10.1042/BSR20181416) proposes a new analytical technique capable to reveal some mechanistic insights into the regulation of insulin-degrading enzyme (IDE), a protein involved in the above-mentioned diseases. IDE is a multifaceted enzyme having different and not well-defined roles in the cell, but it is primarily a proteolytic enzyme capable to degrade several different amyloidogenic substrates involved in different diseases. Moreover, many molecules are responsible for IDE activity modulation so that understanding how IDE activity is regulated represents a very challenging analytical task. The new analytical approach proposed by Ivancic et al. reports on the possibility to study IDE activity in an unbiased and label-free manner, representing a valid alternative assay for the investigation of any proteases degradative activity.

Near-infrared spectroscopy as a potential method for routine sediment analysis to improve rapidity and efficiency

1998 ◽  
Vol 37 (6-7) ◽  
pp. 181-188 ◽  
Author(s):  
Diane F. Malley
Keyword(s):  
Near Infrared ◽  
Organic Matter Content ◽  
Aggregate Size ◽  
Clay Content ◽  
Analytical Techniques ◽  
Matter Content ◽  
Near Infrared Reflectance ◽  
Organic C ◽  
Analytical Technique ◽  
Sediment Analysis

The potential for improvement in the rapidity, cost-effectiveness, and efficiency of sediment analysis by the application of near-infrared reflectance spectroscopy (NIRS) is recognized. The rapid (<2 min), non-chemical, non-destructive analytical technique of near-infrared (700–2500 nm) spectroscopy combines applied spectroscopy and complex statistics. It has been used for the experimental analysis of various constituents and functions of soils since the 1960s, and applications for the analysis of sediments are currently being explored. For application of NIRS, sediment samples require little preparation, other than drying, and the samples are not subject to the manipulations of conventional analytical techniques. The spectral information recorded in a 2 min scan can be used to predict numerous constituents and parameters on the samples once appropriate calibration equations have been prepared from sets of samples analyzed by both NIRS and conventional analytical techniques. Constituents and properties of soil and/or sediment analyzed by NIRS technology include moisture, organic matter content, organic C, CO3=, N, P, S, K, Ca, Mg, clay content, humic acids, lignin, cellulose, metal oxides, heavy metals, aggregate size, and inferred past pH of lakes. Several areas are identified where further research is needed to prepare for the application of NIRS to the routine analysis of sediments.

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