scholarly journals Commentary on Ivancic et al.: Enzyme kinetics from circular dichroism of insulin reveals mechanistic insights into the regulation of insulin-degrading enzyme

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Giuseppe Grasso
Keyword(s):  
Analytical Techniques ◽  
Label Free ◽  
Insulin Degrading Enzyme ◽  
Degrading Enzyme ◽  
Analytical Technique ◽  
Biochemical Pathways ◽  
New Methods ◽  
The World ◽  
Free Environment ◽  
Enormous Number

Despite the enormous number of therapeutic advances in medicine, nowadays many diseases are still incurable, mainly due to the lack of knowledge of the pathological biochemical pathways triggering those diseases. For this reason, it is compulsory for the scientific community to investigate and unveil the biomolecular mechanisms responsible for the development of those diseases, such as Alzheimer’s disease and diabetes, which are widespread all over the world. In this scenario, it is of paramount importance to develop new analytical techniques and experimental procedures that are capable to make the above-mentioned investigations feasible. These new methods should allow easy performable analysis carried out in a label-free environment, in order to give reliable answers to specific biochemical questions. A recent paper published on Bioscience Reports by Ivancic et al. (https://doi.org/10.1042/BSR20181416) proposes a new analytical technique capable to reveal some mechanistic insights into the regulation of insulin-degrading enzyme (IDE), a protein involved in the above-mentioned diseases. IDE is a multifaceted enzyme having different and not well-defined roles in the cell, but it is primarily a proteolytic enzyme capable to degrade several different amyloidogenic substrates involved in different diseases. Moreover, many molecules are responsible for IDE activity modulation so that understanding how IDE activity is regulated represents a very challenging analytical task. The new analytical approach proposed by Ivancic et al. reports on the possibility to study IDE activity in an unbiased and label-free manner, representing a valid alternative assay for the investigation of any proteases degradative activity.

Fragments of the Past

2017 ◽  
Vol 5 (1) ◽  
pp. 71-92 ◽  
Author(s):  
Bradley J. Parker ◽  
Nicola Sharratt
Keyword(s):  
North America ◽  
Analytical Techniques ◽  
Archaeological Sites ◽  
Analytical Technique ◽  
The Past ◽  
The World ◽  
Promising Solution ◽  
Two Phases ◽  
Do So ◽  
Activity Areas

ABSTRACTMicroartifact analysis, the study of the density and distribution of tiny (yet visible) fragments of ceramics, bone, worked stone, and other microartifacts, offers a promising solution to the challenges of determining the location and nature of activity areas at archaeological sites. In spite of the fact that microartifact analysis has been successfully applied at sites in North America and the Middle East, archaeologists have yet to recognize the utility of this methodology in most of the rest of the world. The purpose of this article is, therefore, to test whether this methodology can be profitably applied in the southern Andes. To do so, we describe the results of microartifact sampling, processing, and analytical techniques that we applied to two phases of occupation at the site of Tumilaca la Chimba in southern Peru. The results of the research outlined in this article suggest that microartifact analysis is an effective analytical technique that can contribute greatly to archaeological praxis.

Basic Andragogy-Oriented Strategies and Communication in Online Education

2020 ◽  
Vol 26 (2) ◽  
pp. 350-356
Author(s):  
Anca Sîrbu
Keyword(s):  
Adult Learners ◽  
Online Education ◽  
Social Status ◽  
Rapid Onset ◽  
Learning Goals ◽  
The Novel ◽  
Academic Education ◽  
Online Instructors ◽  
New Methods ◽  
The World

AbstractWith the rapid onset of an unprecedented lifestyle due to the new coronavirus COVID-19 the world academic scene was forced to reform and adapt to the novel circumstances. Although online education cannot be regarded as a groundbreaking endeavour anymore in the21st century, its current character of exclusivity calls for deeper understanding of, and a sharper focus on the “end-consumer” thereof as well as more cautious procedures to be exercised while teaching. While millennials are no longer thought of as being born with a silver spoon in their mouth but with an iPad or any sort of device in their hand (irrespective of their social status), adults are more hesitant when coerced to alter course unexpectedly and turn to new methods of attaining their learning goals. This is why proper communicative approaches need to be thoroughly considered by online instructors. This article aims at presenting teachers with a set of strategies to employ when the beneficiaries of online academic education are adult learners.

Screening and evaluation of Terminalia catappa and its compound application

2020 ◽  
Vol 10 (4) ◽  
pp. 89-92
Author(s):  
Sravani G ◽  
Linga Naik A ◽  
Kranthi A ◽  
Priyanka G
Keyword(s):  
Medicinal Plants ◽  
Conventional Therapy ◽  
Significant Aspect ◽  
Terminalia Catappa ◽  
Specific Disease ◽  
Common Item ◽  
Conventional Medication ◽  
The World ◽  
Enormous Number

Plant determined medications remains an important source, particularly in creating countries, to look at genuine sicknesses roughly 62-80% of the total populace although everything depends on conventional medication for the therapy of specific disease. Indeed, plants produce a various scope of bioactive atoms creation them an ironic wellspring of an alternate kind of drugs. There are hardly any reports and utilization of plants in conventional mending by either ancestral individuals or indigenous network. Rejuvenating plants are the wellspring of extraordinary monetary estimation of everywhere on over the world. Nature has the best word on us a rich plant riches, and an enormous number of assorted kinds of plants develop in various pieces of the nation. Homegrown medication is as yet a pillar of around 75 to 85% the entire populace and the significant aspect of the conventional therapy the utilization of the plant extricate and the dynamic constituents. Among the 7000 types of rejuvenating plants perceived everywhere on over the world in excess of 9000, valuable medicinal plants are found in India. Unfortunately, just not many of them are utilized for their therapeutic worth. Around 1500 plants systematically utilize the conventional arrangement of Indian medication. Notwithstanding, the ethanopharmacologist, microbiologist, botanist and common item physicist world over today, is continually still looking for therapeutic adequacy of the plants on the phytochemicals. Along these lines, the quest for the new phytochemical is the foremost significant important to research the primer phytochemical examination to Terminalia catappa and Syzygium jumbolanam.

Characteristics of Insulin-degrading Enzyme in Alzheimer's Disease: A Meta-Analysis

2018 ◽  
Vol 15 (7) ◽  
pp. 610-617 ◽  
Author(s):  
Huifeng Zhang ◽  
Dan Liu ◽  
Huanhuan Huang ◽  
Yujia Zhao ◽  
Hui Zhou
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Enzyme Activity ◽  
Protein Level ◽  
Senile Plaque ◽  
Meta Analysis ◽  
Cochrane Library ◽  
Insulin Degrading Enzyme ◽  
Degrading Enzyme ◽  
Significant Difference

Background: β-amyloid (Aβ) accumulates abnormally to senile plaque which is the initiator of Alzheimer's disease (AD). As one of the Aβ-degrading enzymes, Insulin-degrading enzyme (IDE) remains controversial for its protein level and activity in Alzheimer's brain. Methods: The electronic databases PubMed, EMBASE, The Cochrane Library, OVID and Sinomed were systemically searched up to Sep. 20th, 2017. And the published case-control or cohort studies were retrieved to perform the meta-analysis. Results: Seven studies for IDE protein level (AD cases = 293; controls = 126), three for mRNA level (AD cases = 138; controls = 81), and three for enzyme activity (AD cases = 123; controls = 75) were pooling together. The IDE protein level was significantly lower in AD cases than in controls (SMD = - 0.47, 95% CI [-0.69, -0.24], p < 0.001), but IDE mRNA and enzyme activity had no significant difference (SMD = 0.02, 95% CI [-0.40, 0.43] and SMD = 0.06, 95% CI [-0.41, 0.53] respectively). Subgroup analyses found that IDE protein level was decreased in both cortex and hippocampus of AD cases (SMD = -0.43, 95% CI [-0.71, -0.16], p = 0.002 and SMD = -0.53, 95% CI [-0.91, -0.15], p = 0.006 respectively). However, IDE mRNA was higher in cortex of AD cases (SMD = 0.71, 95% CI [0.14, 1.29], p = 0.01), not in hippocampus (SMD = -0.26, 95% CI [-0.58, 0.06]). Conclusions: Our results indicate that AD patients may have lower IDE protease level. Further relevant studies are still needed to verify whether IDE is one of the factors affecting Aβ abnormal accumulation and throw new insights for AD detection or therapy.

Recent Developments in CE-MS Based Metabolomics

2020 ◽  
Vol 16 ◽  
Author(s):  
Mustafa Çelebier ◽  
Merve Nenni
Keyword(s):  
Sample Preparation ◽  
Scientific Information ◽  
Analytical Techniques ◽  
Living Organism ◽  
Analytical Technique ◽  
Ionic Compounds ◽  
Recent Sample ◽  
Recent Developments ◽  
Endogenous Metabolites ◽  
Preparation Techniques

Background: Metabolomics has gained importance in clinical applications over the last decade. Metabolomics studies are significant because the systemic metabolome is directly affected by disease conditions. Metabolome-based biomarkers are actively being developed for early diagnosis and to indicate the stage of specific diseases. Additionally, understanding the effect of an intervention on a living organism at the molecular level is a crucial strategy for understanding novel or unexpected biological processes. Results: The simultaneous improvements in advanced analytical techniques, sample preparation techniques, computer technology, and databank contents has enabled more valuable scientific information to be gained from metabolomics than ever before. With over 15,000 known endogenous metabolites, there is no single analytical technique capable of analyzing the whole metabolome. However, capillary electrophoresis-mass spectrometry (CE-MS) is a unique technique used to analyze an important portion of metabolites not accessible by liquid chromatography or gas chromatography techniques. The analytical capability of CE, combined with recent sample preparation techniques focused on extracting polar-ionic compounds, make CE-MS a perfect technique for metabolomic studies. Conclusion: Here, previous reviews of CE-MS based metabolomics are evaluated to highlight recent improvements in this technique. Specifically, we review papers from the last two years (2018 and 2019) on CE-MS based metabolomics. The current situation and the challenges facing metabolomic studies are discussed to reveal the high potential of CE-MS for further studies, especially in biomarker development studies.

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