CORRELATION OF CREATINE KINASE AND MYOGLOBIN CONCENTRATIONS IN PATIENTS SUFFERING FROM DEBILITATED CONDITIONS RELATED TO MYOPATHIES

The present study describes the determination of a possible correlation between CK and myoglobin levels, in addition to the hypothesis that increase in myoglobin concentrations may precede changes in CK levels in patients suffering from myopathies. The inclusion period for this cohort study was from December 2005 to December 2007. Patients who met the laid down and referred criteria were eligible for the study, which includes patients aged > 20 years to < 75 years (both gender) and had a diagnosis of myopathies as per description. Standard protocols were followed for blood samples collection and estimation of aldolase, CK AST, ALT, CK and myoglobin levels. Regression correlations were determined and results were compared as per R2 and significance level P < 0.05. A total of 48 patients were included in the study, males = 32 (66.66%) and females = 16 (33.33%). Except for both CK and myoglobin, the rest of the enzymatic components were not found to be linearly correlated showing R2 factor less than 0.0012. Notably CK and myoglobin levels, when plotted through regression, were leading with exceptional correlation, with R2 0.491 for total patients, R2 0.512 for males and 0.532 for females. It was suggested that by combining both CK and myoglobin estimation in patients with myopathies especially dermatomyositis or polymyositis, it provides better overall impression of muscle damage and related conditions. Correlation of CK and Mb levels in myopathies.

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Amino Acids Changes and Muscle Damage During the 400 km Ultra Trail Gobi Race

10.1101/466706 ◽

2018 ◽

Author(s):

Meng Li ◽

Yao Wu ◽

Guangyuan Huang ◽

Song Chen ◽

Shengting Li ◽

...

Keyword(s):

Amino Acids ◽

Creatine Kinase ◽

Amino Acid ◽

Lactate Dehydrogenase ◽

Muscle Damage ◽

Male Athletes ◽

Progressive Decline ◽

Blood Samples ◽

Mean Values ◽

Ultra Endurance

Current researches demonstrated that completing the Ultra Trail Gobi (UTG) could lead to severe muscle damage. Our study was designed to analysis the muscle damage and amino acid changes reacted to a 400 km ultra-endurance race in experienced runners. Peripheral blood samples from 16 male athletes (mean age 40.3 ± 7.0 years, mean finish time 121.2 ± 21.8 hours), taken 48 h before and immediately after completing the Ultra Trail Gobi Race (UTG), were analyzed for 39 amino acids, 15 steroid hormones and 4 muscle damage factors. In all participants, the 4 biomarkers for muscle damage, i.e., creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) increased significantly after the race, whose mean post-race values were 13.7-, 7.3-, 4.7- and 1.5-fold higher than the pre-race values, respectively. 5 amino acids, i.e., alanine, valine, proline, ornithine and citrulline showed significant decrease, whose mean values decreased by 40.4 ± 18.7%, 38.9 ± 9.3%, 48.1 ± 15.2%, 44.8 ± 15.1% and 23.4 ± 30.8% after the race, respectively. Our study revealed that progressive decline in amino acids contents may further contribute to the factors increasing the muscle damage during the UTG.

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Effects of Stocking Density of Lambs on Biochemical Stress Parameters and Meat Quality Related to Commercial Transportation

Annals of Animal Science ◽

10.2478/aoas-2014-0012 ◽

2014 ◽

Vol 14 (3) ◽

pp. 611-621 ◽

Cited By ~ 9

Author(s):

Bulent Teke ◽

Bulent Ekiz ◽

Filiz Akdag ◽

Mustafa Ugurlu ◽

Gulay Ciftci ◽

...

Keyword(s):

Creatine Kinase ◽

Meat Quality ◽

Stress Responses ◽

Shear Force ◽

Stocking Density ◽

Quality Characteristics ◽

Blood Samples ◽

Colour Parameters ◽

Stress Parameters

AbstractThe aim of this study was to evaluate the effects of stocking density (0.20 and 0.27 m2/lamb) during transportation on certain biochemical stress parameters and meat quality characteristics of fifty- five Karayaka lambs divided into two groups. The front compartment of the trailer was loaded at high stocking density (hd: 0.20 m2/lamb; n=33) and the remainder was loaded at low stocking density (ld: 0.27 m2/lamb; n=22). the distance was approximately 130 km and duration was 2 h 15 min. blood samples were taken just before and just after transportation. glucose (p<0.001), lactate (p<0.001), cortisol (p<0.01), creatine kinase (ck) (p<0.01), lactate dehydrogenase (ldh) (P<0.01) and alanine aminotransferase (ALT) (P<0.05) levels were significantly higher in lambs of the hd group than in the ld group. the effects of stocking density on ph, proportion of expressed juice, cooking loss, Warner-Bratzler shear force value and meat colour parameters were not significant (P>0.05). Transportation with a stocking density of 0.20 m2/lamb resulted in higher stress responses in lambs compared with a stocking density of 0.27 m2/lamb. however, increased stress in the hd group did not lead to any adverse effects on meat quality characteristics. in the determination of stocking density, a compromise that gives animal welfare and commercial objectives equal status would improve the current situation.

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Effort of Varying Intensity as a Factor Influencing the Variability of Selected Biochemical Blood Parameters of Jumping Horses

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/v10213-012-0040-2 ◽

2012 ◽

Vol 56 (2) ◽

pp. 225-229 ◽

Cited By ~ 4

Author(s):

Hanna Bis-Wencel ◽

Krzysztof Lutnicki ◽

Agnieszka Zofia Rowicka ◽

Bożena Nowakowicz-Dębek ◽

Monika Bryl

Keyword(s):

Alkaline Phosphatase ◽

Creatine Kinase ◽

Muscle Damage ◽

Muscle Tissue ◽

Blood Parameters ◽

Biochemical Properties ◽

Blood Samples ◽

Time Points ◽

The Difference ◽

Intensity Characteristic

Abstract Blood samples were collected from horses in four time points of the spring-winter training season, according to the difference in effort intensity characteristic for each period. Serum contents of glucose, cholesterol, and creatinine, as well as activity of creatine kinase, aspartate and alanine aminotransferases, and alkaline phosphatase were determined. Training did not cause muscle damage, and as a result of training, changes in the characteristics of both, contraction and biochemical properties of muscle tissue took place, which was confirmed by the conducted tests.

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Effects of combined carbohydrate and protein supplementation on athletic performance and recovery of endurance runners：a double blind, random trial

10.21203/rs.3.rs-330022/v1 ◽

2021 ◽

Author(s):

Yiheng Liang ◽

Fan Yang ◽

Yan Chen ◽

Guoqiang Geng ◽

Mingyue Lu ◽

...

Keyword(s):

Creatine Kinase ◽

Muscle Damage ◽

Endurance Exercise ◽

Athletic Performance ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Protein Supplementation ◽

Double Blind ◽

Blood Samples ◽

Significant Difference

Abstract Background The aim of this study is to explore the effects of ingesting protein supplementation before endurance exercise. Methods 10 recreationally active male runners (VO2max: 53.61±3.86 ml/kg•min) completed a run-to-exhaustion test three times. Each test involved 90 minutes of running at 70% VO2max, followed by a time to exhausted test for running at 80% VO2max. All subjects ingested three different, randomly assigned, supplement before the first phase. At the end of the first phase, CHO (carbohydrate) + CHO; PRO (protein) + CHO; CHO + PRO. Both carbohydrate and protein were supplemented at 0.4g•kg− 1BM− 1. Blood samples were obtained before, immediately after and 24 h after exercise for alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), and myoglobin (MB). Results Three different supplement regimens did not cause a significant difference in exhaustion time (CHO + CHO: 432.36 ± 225.51s; PRO + CHO: 463.82 ± 227.45s; CHO + PRO: 461.45 ± 248.5s). However, ALT and AST in PRO + CHO were significantly lower than CHO + CHO 24h after exercise (ALT: 16.8 ± 6.31 VS. 24.39 ± 2.54 U/L; AST: 24.06 ± 4.77 VS. 31.51 ± 7.53 U/L, p < 0.05), and MB in PRO + CHO and CHO + PRO were significantly lower than CHO + CHO 24 h after exercise (40.71 ± 15.16; 38.12 ± 14.32; 64.32 ± 28.86 ng/mL, respectively, p < 0.05). Compared to CHO + CHO, CK in PRO + CHO increased less 24 h after exercise (404.22 ± 75.31 VS. 642.33 ± 68.57 U/L, p < 0.05). Conclusion Although combined carbohydrate and protein supplementation did not prolong exhaustion time, it can effectively relieve muscle damage, and it is better to supplement PRO before exercise.

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Effect of Immersion in Cold Water on Creatine Kinase and Myoglobin Levels in Soccer Players

Journal of Health Sciences ◽

10.17921/2447-8938.2020v22n2p151-155 ◽

2020 ◽

Vol 22 (2) ◽

pp. 151-155

Author(s):

Fernanda Bortolo Pesenti-Tofalini ◽

Eduardo Rossi Spartalis ◽

Alessandra Miyuki Okino ◽

Danielle Venturini ◽

Ariobaldo Frisseli ◽

...

Keyword(s):

Creatine Kinase ◽

Muscle Damage ◽

Room Temperature ◽

Cold Water ◽

Intervention Group ◽

Athletic Injuries ◽

Soccer Players ◽

Control Group ◽

Blood Samples ◽

And Control

The objective of this study was to analyze the effect of body immersion in cold water on creatine kinase and myoglobin levels in soccer athletes. For this, 10 soccer players aged between 16 and 18 years old, submitted to 5 blood samples, were analyzed: fasting, post-match, after 24, 48 and 72 hours. At the end of the match they were randomized into intervention group (GI) and control (CG). GI immersion in cold water, water plate at the level of the iliac crests, 10 ° C, for 10 minutes and the GC immersed in water at room temperature at the same height and for the same period of time. The levels of creatine kinase did not change in the analyzed groups and moments. Myoglobin levels increased significantly after the match and returned to baseline after 24 and 72 hours, with a peak in 48 hours. There was no difference between the intervention groups at all the evaluated moments. It was concluded that immersion in cold water did not alter the creatine kinase and myoglobin levels in relation to the control group in soccer athletes, indicating that for this population immersion in cold water was not able to reduce muscle damage after exercise. Keywords: Athletic Injuries. Myalgia. Fatigue. Cryotherapy. Resumo O objetivo deste estudo foi analisar o efeito da imersão corporal em água fria nos níveis de creatinoquinase e mioglobina em atletas de futebol. Para isto, foram analisados 10 atletas de futebol com idade entre 16 e 18 anos, submetidos à 5 coletas sanguíneas: em jejum, após jogo, após 24, 48 e 72 horas. Ao término do jogo foram aleatorizados em grupo intervenção (GI) e controle (GC). O GI realizou imersão em água fria, lâmina da água à altura das cristas ilíacas, 10ºC, por 10 minutos e o GC realizou imersão em água à temperatura ambiente à mesma altura e durante o mesmo período de tempo. Os níveis de creatinoquinase não apresentaram alteração nos grupos e momentos analisados. Já os níveis de mioglobina aumentaram significativamente após o jogo e retornaram aos valores basais após 24 e 72 horas, com pico em 48 horas. Não houve diferença entre os grupos de intervenção em todos os momentos avaliados. Conclui-se então que a imersão em água fria não alterou os níveis de creatinoquinase e mioglobina em relação ao grupo controle em atletas de futebol, indicando que para esta população a imersão em água fria não foi capaz de reduzir o dano muscular após exercício. Palavras-chave: Traumatismos em Atletas. Mialgia. Fadiga. Crioterapia.

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Investigation of the Effects of Volleyball Training on Athletes' Liver Enzymes and Muscle Damage Markers

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i831207 ◽

2021 ◽

pp. 1-6

Author(s):

Ramazan Erdoğan

Keyword(s):

Creatine Kinase ◽

Muscle Damage ◽

Alanine Aminotransferase ◽

Liver Enzymes ◽

Physiological Changes ◽

Test Results ◽

Blood Samples ◽

Creatine Kinase Mb ◽

Significant Difference ◽

Post Test

Aims: It is seen that regular exercises cause physiological changes in the organism. The effects of such a training are not known especially on liver enzymes and muscle damages. In this regard, this study aimed to determine the effects of long-term volleyball training on athletes’ indicators of liver enzymes and muscle damages. Methodology: 20 male volunteer athletes in volleyball branch participated in the study. In the study, a 12-week training program was applied to the athletes four days a week, and one day intended for conditioning training in each week. Blood samples were received from the athletes for two times when they were resting that was before and at the end of the training. Indicators of liver enzymes and muscle damages of athletes were determined in these blood samples received. The data obtained in this study were analysed by SPSS 22 package program. Paired Samples t-test was used in the comparison of pre-post test data of the study group. Significance level was accepted to be p<0,05. Results: After the data evaluated, it was determined that there was a statistically significant difference between the pre and post-test results of muscle damage indicators and CK (Creatine Kinase) and CK-MB (Creatine Kinase-MB) values of the athletes participating in the study (p<0,05). Considering the pre and post-test results of the athletes’ liver enzymes, a statistically significant difference was found to be at LDH (Lactate Dehydrogenase), AST (Aspartate Aminotransferase), ALT (Alanine Aminotransferase) and ALP (Alanine aminotransferase) levels (p<0,05) while there was no statistically significant difference at GGT (Gamma Glutamyl Transpeptidase) levels. Conclusion: As a result, regular volleyball training was determined to cause changes in the athletes’ indicators of liver enzymes and muscle damages. Considering the physiological changes caused by the training programs to be applied, it is thought that the performances of the athletes will be positively affected.

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Determination of factors involved in the rejection of bananas (Musa acuminata) intended for international commercialization

10.37281/preprints/2 ◽

2020 ◽

Keyword(s):

Management Practices ◽

Abiotic Factors ◽

Quality Standards ◽

Musa Acuminata ◽

P Value ◽

Biotic Factors ◽

Significance Level ◽

Export Sector ◽

Export System

The banana agro-export sector in Ecuador provides millions of dollars in income for this concept, but with this development, a series of quality standards have been established that must be met to enter the export system. This has contributed to establishing good post-harvest production and management practices that guarantee the optimal production of bananas and plantains. The objective of this study was to determine the factors involved in the rejection of bananas (Musa acuminata) destined for international commercialization. The methodology considered the design modality of non-experimental transactional research, with a quantitative approach. The methodological design was developed in three phases at Finca 6 Hermanas located in the Barraganete sector of the San Juan parish in the Puebloviejo canton of the Los Ríos Province, Ecuador. The results highlight that the main causes for which banana rejection is generated are due to abiotic factors (damage, dry latex, scar, insect damage, broken neck, overgrowth) in a higher percentage of 79.55 % and biotic factors ( twins, diseases, short finger) by 20.45 %. The average rejection was 6 361 fingers and1 269 Kilograms (K) over the 6-week study duration. The analysis of variance turned out to be significant for variable 1 (biotic and abiotic). Ho is rejected; with the criterion of p-value < 0.0001 and F (9; 45) = 2.10., F = 13.17> F critic. In the case of variable (2) “work weeks”, Ho is accepted with the criteria obtained of p-value of 0.7694 and F (5; 45) = 2.4., As F = 0.51 < F critic, it is concludes, that with a significance level of 5% the null hypothesis is accepted. It is concluded that these figures lead to the elaboration of strategies that systemically mitigate the damages, by correcting each one of the causes that cause the deterioration of the banana and increasing the economic gains of the commercialization process.

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Synthesis and solvatochromic studies of 2- amino-3-phenolazo1-(4-sulfophenyl)-3-methyi-5-pyrozolone and use it for the determination of trace amount of Nickel (II) in blood samples

International Journal of Bioassays ◽

10.21746/ijbio.2016.10.001 ◽

2016 ◽

Vol 5 (10) ◽

pp. 4920

Author(s):

Amar M. Ali ◽

Hussain. J. Mohammed*

Keyword(s):

Stability Constant ◽

Trace Amount ◽

Benzenesulfonic Acid ◽

Determination Method ◽

Blood Samples ◽

Determination Of Nickel ◽

Normal Human ◽

The Stability ◽

Normal Human Blood

A new, simple, sensitive and rapid spectrophotometric method is proposed for the determination of trace amount of Nickel (II). The method is based on the formation of a 1:2 complex with 4-(4-((2-hydroxy-6-nitrophenyl) diazenyl) -3-methyl-5-oxo-2, 5-dihydro-1H-pyrazol-1-yl) benzenesulfonic acid (2-ANASP) as a new reagent is developed. The complex has a maximum absorption at 516 nm and εmax of 1. 84 X 105 L. mol-1. cm-1. A linear correlation (0. 25 – 4. 0μg. ml-1) was found between absorbance at λmax and concentration. The accuracy and reproducibility of the determination method for various known amounts of Nickel (II) were tested. The results obtained are both precise (RSD was 1. 2 %) and accurate (relative error was 0. 787 %). The effect of diverse ions on the determination of Nickel (II) to investigate the selectivity of the method were also studied. The stability constant of the product was 0. 399 X 106 L. mol-1. The proposed method was successfully applied to the analysis of diabetes blood and normal human blood.

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Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽

10.3390/separations8060078 ◽

2021 ◽

Vol 8 (6) ◽

pp. 78

Author(s):

Sevasti Karampela ◽

Jessica Smith ◽

Irene Panderi

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Formic Acid ◽

Whole Blood ◽

High Performance ◽

Tandem Mass ◽

Psychoactive Substances ◽

Blood Samples ◽

Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

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145 President Oral Presentation Pick: Prenatal stress increases skeletal muscle mitochondrial volume density and function in yearling Brahman calves

Journal of Animal Science ◽

10.1093/jas/skaa278.219 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 120-121

Author(s):

Chloey P Guy ◽

Catherine L Wellman ◽

David G Riley ◽

Charles R Long ◽

Ron D Randel ◽

...

Keyword(s):

Oxidative Stress ◽

Creatine Kinase ◽

Muscle Damage ◽

Prenatal Stress ◽

Citrate Synthase ◽

Volume Density ◽

Assembly Factor ◽

Mitochondrial Volume ◽

Mitochondrial Volume Density ◽

And Function

Abstract We previously determined that prenatal stress (PNS) differentially affected methylation of DNA from leukocytes of 28-d-old calves. Specifically, COX14 (cytochrome c oxidase (COX) assembly factor) and CKMT1B (mitochondrial creatine kinase U-type) were hypomethylated and COA5 (COX assembly factor 5), COX5A (COX subunit 5A), NRF1 (nuclear respiratory factor 1), and GSST1 (glutathione S-transferase theta-1) were hypermethylated in PNS compared to non-PNS calves (P ≤ 0.05). Our current objective was to test the hypothesis that PNS exhibit impaired mitochondrial function and greater oxidative stress than non-PNS calves. Blood and longissimus dorsi muscle samples were collected from yearling Brahman calves whose mothers were stressed by 2 h transportation at 60, 80, 100, 120, and 140 days of gestation (PNS; 8 bulls, 6 heifers) and non-PNS calves (4 bulls, 6 heifers). Serum was evaluated for the stress hormone, cortisol, and muscle damage marker, creatine kinase; muscle was analyzed for mitochondrial volume density and function by citrate synthase (CS) and COX activities, respectively, concentration of malondialdehyde, a lipid peroxidation marker, and activity of the antioxidant, superoxide dismutase (SOD). Data were analyzed using mixed linear models with treatment and sex as fixed effects. Serum cortisol was numerically higher in PNS than non-PNS calves but was not statistically different. Muscle CS and COX activities relative to protein were greater in PNS than non-PNS calves (P ≤ 0.03), but COX relative to CS activity was similar between groups. Activity of COX was greater in bulls than heifers (P = 0.03), but no other measure was affected by sex. All other measures were unaffected by PNS. Prenatal stress did not affect markers of muscle damage and oxidative stress in yearling Brahman calves at rest but mitochondrial volume density and function were greater in PNS calves. Acute stressors induce oxidative stress, so implications of differences in mitochondria in PNS calves following a stressor should be investigated.

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