scholarly journals Evaluation Activity of Alanine Aminopeptidase (AAP) in Patients with Renal Dialysis, Purification and Isolation of Isoenzymes

2019 ◽  
pp. 265-272
Author(s):  
Taghreed Mohammad ◽  
Sawsan Mahdi ◽  
Tammara Al-Kareim
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Renal Dialysis ◽  
Exchange Chromatography ◽  
Aminopeptidase Activity ◽  
Control Group ◽  
Efficacy Rate ◽  
Loss Of Function ◽  
Evaluation Activity ◽  
Alanine Aminopeptidase

Chronic renal failure is the progressive loss of function of kidney and patient requires a long treatment in the form of renal replacement therapy. Alanine aminopeptidase activity has been assayed in (70) patient’s sera samples of both sexes, aged (25-70) years. The sample were divided in to two groups, the first group(G1) is of (35) samples before dialysis. The second group(G2) is of (35) samples after dialysis, as well as (30) sample of healthy as control group(C). The result showed a significant increase (p> 0.001) in general in the enzyme Alanine aminopeptidase in the serum of chronic renal failure patients (116.0±3.4) U/L while the efficacy rate is (105.5±3.4) U/L in healthy persons. Goal of the research was partial purified of enzyme from sera patients with chronic renal failure. by using two techniques: The first gel filtration chromatography packed with spadix G50 and giving 3.45% yield and 0.964-fold purification. Two isoenzymes were obtained by using ion exchange chromatography and the purity degree of isoenzymes were 0.687(1.08%) and 2.539(1.04%) fold respectively. Also, the results showed a single band for isoenzymes following steps.

scholarly journals Effect of Video Assisted Teaching on Knowledge of BSc Nursing Students regarding Renal Dialysis for Chronic Renal Failure Patients

2021 ◽  
Vol 10 (01) ◽  
pp. 1-6
Author(s):  
Abha Oriel ◽  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Nursing Students ◽  
Renal Dialysis ◽  
Control Group ◽  
Knowledge Gain ◽  
Video Assisted ◽  
Post Test ◽  
And Control ◽  
Experimental Group

Background: The present study was conducted to assess the effect of video assisted teaching on the knowledge gain of BSc Nursing students on renal dialysis for chronic renal failure patients. Methods: An experimental design was chosen with pre test and post test of experimental and control group. The sample size was 60 registered BSc Nursing students divided into two groups - 30 in the experimental and 30 in the control group. The tools used for conducting the study included demographic variables, self structured questionnaire to assess knowledge of experimental and control group. The experimental group was given video- assisted teaching as an intervention and the control group was used for comparison only. The data were analysed with the help of descriptive and inferential statistics. Result: The study clearly shows that there was a significant gain in knowledge of BSc Nursing students in experimental group with video assisted teaching which emphasises that renal dialysis for chronic renal failure patients is good for their health. Conclusion: The BSc Nursing students can be benefited with video assisted teaching to improve knowledge and practice on renal dialysis for Chronic Renal Failure Patients and they can practice these interventions in clinical area in future.

Effect of Proportional Reduction of Sodium Intake on the Adaptive Increase in Glomerular Filtration Rate/Nephron and Potassium and Phosphate Excretion in Chronic Renal Failure in the Rat

1975 ◽  
Vol 49 (3) ◽  
pp. 193-200 ◽  
Author(s):  
C. H. Espinel
Keyword(s):  
Renal Failure ◽  
Glomerular Filtration Rate ◽  
Chronic Renal Failure ◽  
Glomerular Filtration ◽  
Sodium Excretion ◽  
Filtration Rate ◽  
Sodium Intake ◽  
Dietary Sodium ◽  
Control Group ◽  
Phosphate Excretion

1. The influence of dietary sodium intake on the glomerular filtration rate (GFR/nephron) and potassium and phosphate excretion was examined at three stages of progressive chronic renal failure produced in rats by sequential partial nephrectomies. 2. The adaptive increased sodium excretion per nephron in the control group receiving a constant sodium intake did not occur in the experimental group that had a gradual reduction of dietary sodium in direct proportion to the fall in GFR. 3. Despite the difference in sodium excretion, the increase in GFR/nephron, the daily variation in the amount of potassium and phosphate excreted, the increase in potassium and phosphate excretion per unit nephron, and the plasma potassium and phosphate concentrations were the same in the two groups. 4. The concept of ‘autonomous adaptation’ in chronic renal failure is presented.

scholarly journals ROLE OF CARDIAC MARKERS IN CHRONIC RENAL FAILURE PATIENTS.

2020 ◽  
pp. 81-82
Author(s):  
Ramesh Chandra Thanna ◽  
B K Agarwal ◽  
Rakesh Romday ◽  
Neha Sharma
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Positive Association ◽  
Morbidity And Mortality ◽  
Control Group ◽  
High Morbidity ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Hs Crp

Introduction: Cardiovascular diseases (CVD) are known as important reasons of the increased morbidity and mortality observed in patients with chronic renal failure (CRF). The association of serum Interlukin-6 , homocysteine as well as other cardiovascular risk factors in relation to existence and cause of CVD were investigated. Method: In this study 200 CRF patients were recruited and further stratified into group with Male and Female as case groups. Those without renal failure were assigned as control group (n=200). Results: The patients with CRF showed a significant increase in plasma levels of Cpk-MB homocysteine and C-reactive protein (CRP) compared to control. The positive association were observed between homocysteine, Urea and Hs-CRP, IL_6 . It shows a significant Association of parameters in CRF . Conclusion: The results demonstrated elevation in plasma values IL-6 , homocysteine and HS-CRP in patients with CRF . However, these modifications may be lead to atherosclerosis and consequence CVD event. These parameters may be important with respect to the high morbidity and mortality of CVD found in patients with CRF.

scholarly journals Effect of chronic renal failure on the hepatic, intestinal, and renal expression of bile acid transporters

2014 ◽  
Vol 306 (1) ◽  
pp. F130-F137 ◽  
Author(s):  
Zhibo Gai ◽  
Lei Chu ◽  
Christian Hiller ◽  
Denis Arsenijevic ◽  
Carlos A. Penno ◽  
...  
Keyword(s):  
Renal Failure ◽  
Bile Acid ◽  
Chronic Renal Failure ◽  
Plasma Cholesterol ◽  
Experimental Studies ◽  
Control Group ◽  
Early Event ◽  
Minor Role ◽  
Serum Bile Acid ◽  
Protein Levels

Although the kidney is believed to play a minor role in bile acid (BA) excretion, chronic renal failure (CRF) has been reported to be associated with increased serum bile acid levels and alterations in BA homeostasis. The mechanisms for elevated BA levels are poorly understood in both clinical and experimental studies. This study was designed to examine the effects of naturally progressing CRF of longer duration on the hepatic and renal mRNA and protein levels of the BA-synthesizing enzyme Cyp7a1 and the BA transporters Ntcp, Bsep, Mrp3, Ost-α, and Ost-β. Sprague-Dawley rats were randomized to the CRF group (⅚ nephrectomy) or to the sham-operated control group and were analyzed 8 wk after surgery. Results obtained in the CRF rats were compared with those obtained in rats that had undergone uninephrectomy (UNX). The CRF group exhibited significantly increased plasma cholesterol and BA concentrations. Hepatic Cyp7a1 mRNA and protein levels were almost identical in the two groups. Hepatic Mrp3, Ost-α, and Ost-β expression was increased, suggesting increased basolateral efflux of bile acids into the blood. However, no such changes in BA transporter expression were observed in the remnant kidney. In UNX rats, similar changes in plasma BA levels and in the expression of BA transporters were found. We hypothesize that the increase in plasma BA is an early event in the progression of CRF and is caused by increased efflux across the basolateral hepatocyte membrane.

scholarly journals Protein-to-creatinine urinary in the early diagnosis of renal injury in canine pyometra

2019 ◽  
Vol 39 (3) ◽  
pp. 186-191
Author(s):  
Marcos C. Sant’Anna ◽  
Guilherme F. Martins ◽  
Karina K.M.C. Flaiban ◽  
Luiz G.C. Trautwein ◽  
Maria I.M. Martins
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Renal Injury ◽  
Renal Damage ◽  
Systemic Disease ◽  
The Other ◽  
Control Group ◽  
Creatinine Ratio ◽  
Renal Lesions ◽  
Tubular Cells

ABSTRACT: Kidney disease that affects bitches with pyometra may lead patients to develop chronic renal failure even after pyometra treatment. Therefore, several studies have sought to clarify the gaps in the understanding of the pathogenesis of renal injury in pyometra. Identification of early detection markers for renal damage, which can predict and identify the prognosis of the disease, is very important. Proteinuria analysis can diagnose kidney damage, since proteins such as albumin are not filtered through the glomerulus and those that undergo glomerular filtration are almost completely reabsorbed by tubular cells. The objective of this study was to evaluate whether the urinary protein-to-creatinine ratio (UPC) can detect renal injury in bitches with pyometra before development of azotemia. For this, 44 bitches with pyometra were divided into two groups: bitches with azotemic piometra (A, n=15, creatinine >1.7) and bitches with non-azotemic pyometra (NA, n=29). The two groups were compared to the control group (CG, n=12), which had no signs of systemic disease. All animals underwent blood and urine tests. Leukocytosis was more evident in bitches in the A group than in the other groups. This shows that the inflammatory response may be associated with the pathogenesis of renal injury. The median UPC in bitches with pyometra was significantly higher than in the CG, with a median above the reference values. In conclusion, the UPC can be used in bitches with pyometra to detect renal damage before the development of azotemia. It has been suggested that the UPC of bitches with pyometra should be followed through during the postoperative period so that permanent renal lesions secondary to pyometra can be diagnosed and treated properly before the development of azotemia.

scholarly journals iTRAQ-Based Proteomics of Chronic Renal Failure Rats after FuShengong Decoction Treatment Reveals Haptoglobin and Alpha-1-Antitrypsin as Potential Biomarkers

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yu Yang ◽  
Junmeng Wei ◽  
Xuekuan Huang ◽  
Mingjun Wu ◽  
Zhenbing Lv ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Differentially Expressed ◽  
Coagulation Cascade ◽  
Control Group ◽  
Differentially Expressed Proteins ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Alpha 1 Antitrypsin ◽  
Global Health Problem

Background. Chronic renal failure (CRF) has become a global health problem and bears a huge economic burden. FuShengong Decoction (FSGD) as traditional Chinese medicine has multiple pharmacological effects. Objectives. To understand the underlying molecular mechanism and signaling pathway involved in the FSGD treatment of CRF and screen differentially expressed proteins in rats with CRF treated with FSGD. Methods. Thirty-three male Sprague-Dawley rats were randomly divided into control group, CRF group, and FSGD group. Differentially expressed proteins were screened by iTRAQ coupled with nanoLC-MS/MS, and these identified proteins were later analyzed by GO, KEGG, and STRING. Additionally, haptoglobin (HP) and alpha-1-antitrypsin (AAT) were finally verified by ELISA, Western blot, and real time PCR. Results. A total of 417 proteins were identified. Nineteen differentially expressed proteins were identified in the FSGD group compared with the model group, of which 3 proteins were upregulated and 16 proteins were downregulated. Cluster analysis indicated that inflammatory response was associated with these proteins and complement and coagulation cascade pathways were predominantly involved. The validation methods further confirmed that the levels of HP and AAT were significantly increased. Conclusions. HP and AAT may be the important biomarkers in the pathogenesis of CRF and FSGD therapy.

Allantoin as a marker of oxidative stress in human erythrocytes

2008 ◽  
Vol 46 (9) ◽  
Author(s):  
Roman Kand'ár ◽  
Pavla Žáková
Keyword(s):  
Oxidative Stress ◽  
Renal Failure ◽  
Uric Acid ◽  
Chronic Renal Failure ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Control Group ◽  
Uric Acid Concentration ◽  
Reversed Phase Hplc

Abstract: Uric acid is the final product of purine metabolism in humans. It was determined that this compound has important antioxidative properties and it may be oxidized to allantoin by various reactive oxygen species. Therefore, the measurement of allantoin may be useful for the determination of oxidative stress in humans.: We measured allantoin and uric acid in human plasma and erythrocytes obtained from patients with chronic renal failure before hemodialysis (n=30) and blood donors (n=30). We used a method based on selective isolation of allantoin from deproteinized plasma and erythrocyte lysate samples on AG 1-X8 resin and its derivatization to glyoxylate-2, 4-dinitrophenylhydrazone. Separation of glyoxylate-2, 4-dinitrophenylhydrazone from interfering substances was achieved on reversed phase HPLC with gradient elution and UV/VIS detection at 360 nm. Uric acid was determined by reversed phase HPLC with UV/VIS detection at 292 nm.: We found significant differences in allantoin and uric acid concentration between the patients with chronic renal failure and the control group both in plasma (20.5±6.5 μmol/L and 323.9±62.9 μmol/L vs. 2.1±1.1 μmol/L and 270.1±62.3 μmol/L, p<0.05) and erythrocytes [82.8±39.1 nmol/g hemoglobin (Hb) and 110.7±28.8 nmol/g Hb vs. 20.1±6.1 nmol/g Hb and 82.1±23.7 nmol/g Hb, p<0.05].: Significant higher levels of allantoin in both plasma and erythrocytes of patients with chronic renal failure indicate that allantoin may be used as a good marker of oxidative stress.Clin Chem Lab Med 2008;46:1270–4.

PENGARUH KOMUNIKASI TERAPEUTIK TERHADAP HARGA DIRI KLIEN DENGAN GAGAL GINJAL KRONIS

2019 ◽  
Vol 1 (1) ◽  
pp. 28
Author(s):  
Juniyaska Hari Pratama ◽  
Ninuk Dwi Kurniati ◽  
Abu Bakar
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Treatment Group ◽  
Healing Process ◽  
Self Esteem ◽  
Communication Intervention ◽  
Rank Test ◽  
Control Group ◽  
Therapeutic Communication ◽  
Significant Difference

Introduction: Chronic renal failure is clinical syndrome caused by declining renal function, lasting progressively and irreversible. Physical change due to organ function decline will affect client psychologicaly and socially. These changes cause self esteem disorder where client tend to use maladaptive coping with manifestation of frequent irritating, angry, anxiety, and depression. Therapeutic communication between nurse and client that lead to client healing process which planned in conscious, purposive manner and this activity is centered on client. Method: This research used quasi experimental. Total sample in this research was 12 clients with chronic renal failure that meet inclusion criteria grouped in two groups: treatment group and control group. Independent variable was therapeutic communication while dependent variable was self esteem. In this research data analysis used was Wilcoxon Sign Rank Test with significance α ≤ 0,05 and Mann Whitney Test with significance α ≤0,05. Result: Result obtained after therapeutic communication intervention provided was p = 0,002 where there is significant difference on client self esteem before and after therapeutic communication intervention given. Result showed that there is significant difference between treatment group and comparative group with p = 0.026. Discussion: It is concluded that therapeutic communication has effect on improving self esteem in client with chronic renal failure. Therapeutic communication helps client to express his/her feeling and problems clients self esteem were improved.

scholarly journals Perfil Epidemiológico dos portadores de insuficiência renal crônica submetidos à terapia hemodialítica

2014 ◽  
Vol 5 (3/4) ◽  
pp. 65-69
Author(s):  
Ivonizete Pires Ribeiro ◽  
Andira Luana Soares Pinheiro ◽  
Andressa Luana Araújo Soares ◽  
Nayara Franciele Marques Dos Santos
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Data Collection ◽  
Health System ◽  
National Health ◽  
National Health System ◽  
Renal Dialysis ◽  
Underlying Disease ◽  
Epidemiological Profile ◽  
The City

Resumo: Objetivou-se traçar o perfil epidemiológico do portador de IRC em tratamento hemodialítico na cidade de Teresina-PI. Tratou-se de um estudoquantitativo descritivo, realizado na unidade de nefrologia conveniada ao Sistema Único de Saúde (SUS), com 64 pacientes. A coleta de dados ocorreude março a maio do ano de 2010. Constatou-se que a maioria dos pacientes é do gênero masculino (67,19%), faixa etária de 40 a 59 anos (40,63%), sendo60,94% casados, residentes em Teresina (64,06%), com baixo nível de escolaridade, renda individual de 1 a 2 salários mínimos e com moradia própria.Em 69% a doença de base foi hipertensão arterial e diabetes; e as principais dificuldades encontradas foram os efeitos causados pelo tratamento eo transporte, influenciando na manutenção da terapia hemodialítica. Tais fatores servem para subsidiar decisões para melhorar a assistência a essespacientes juntamente com o enfermeiro atuando na prevenção das doenças que levam a IRC.Descritores: Insuficiência Renal Crônica, Diálise Renal, Avaliação em enfermagem.Epidemiological profile of renal failure patients submitted to hemodialysisAbstract: The objective was to outline the epidemiological profile of patients with CRF on hemodialysis in the city of Teresina-PI. This is a descriptivequantitative study, conducted in the nephrology unit convening the National Health System (SUS), with 64 patients. Data collection occurred from Marchto May of 2010. It was found that the majority of patients are male (67.19%), aged 40-59 years (40.63%), and 60.94% were married, living in Teresina(64.06%) with fewer years of education, individual income 1-2 minimum wages and proper housing. In 69% the underlying disease was hypertensionand diabetes, and the principal problems were the effects caused by treatment and transport, influencing the maintenance of hemodialysis. Such factorsserve to support decisions to improve care for these patients jointly with nurses working in the prevention of diseases leading to CRF.Descriptors: chronic renal failure, renal dialysis, nursing assessment.Perfil Epidemiológico dos los pacientes con insuficiencia renal crónica sometidos a hemodiálisisResumen: El objetivo fue analizar el perfil epidemiológico de los pacientes con insuficiencia renal crónica en hemodiálisis en la ciudad de Teresina-PI.Este es un estudio cuantitativo descriptivo, realizado en la unidad de nefrología convocatoria del Sistema Nacional de Salud (SUS), con 64 pacientes. Losdatos fueron recolectados entre marzo y mayo de 2010. Se encontró que la mayoría de los pacientes son hombres (67,19%), edad 40-59 años (40,63%), y60,94% estaban casadas, que viven en Teresina (64,06%) con menos años de educación, los ingresos individuales de 1.2 salarios mínimos y una viviendadigna. En un 69% la enfermedad de base fue la hipertensión y la diabetes, y los principales problemas fueron los efectos causados por el tratamientoy el transporte, que influyen en el mantenimiento de la hemodiálisis. Estos factores sirven para apoyar las decisiones para mejorar la atención de estospacientes junto con las enfermeras que trabajan en la prevención de las enfermedades que conducen a la IRC.Descriptores: Insuficiencia renal crónica, La diálisis renal, Evaluación en enfermería.

scholarly journals The Study of Biochemical Markers in Chronic Renal Failure Patients in Pre- and Post-Haemodialysis

2021 ◽  
Vol 53 (03) ◽  
pp. 259-264
Author(s):  
Mohammed Abed Jawad ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Renal Failure ◽  
Chronic Renal Failure ◽  
Group Method ◽  
Control Group ◽  
Renal Markers ◽  
Patients With Diabetes ◽  
History Of ◽  
Notable Increase

Background: The current research is designed to investigate alterations in lipid peroxidation (malondialdehyde - MDA) and renal markers (urea and creatinine) in patients of chronic renal failure (CRF) as compared to the control group. Method: The study included 55 subjects, who were separated into two groups: control group, which included 15 healthy members with no history of systematic illness; and patients group, which included 40 patients with CRF divided into four groups “Non: CRF patients without any accompanied disease, DM: CRF patients with diabetes mellitus, HT: CRF patients with hypertension, and HT + DM: CRF patients with diabetes mellitus and hypertension”. Results: The findings reveal that there is a notable increase in serum concentration of MDA, urea, and creatinine, in patients group as compared to the control group. Conclusion: In all chronic renal failure patients with or without any accompanying disease, lipid peroxidation is present in pre- and post-haemodialysis patients as well as patients with CRF have high levels of urea and creatinine compared with healthy groups.

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