Studi Perbandingan Kemampuan Nannochloropsis sp. Dan Spirulina sp. Sebagai Agen Bioremediasi Terhadap Logam Berat Timbal (Pb) [Comparative Study Of Ability Nannochloropsis sp. And Spirulina sp. As Agent Bioremediation Of Heavy Metal Plumbum (Pb) ]

Abstract Microalgae species Nannochloropsis sp. can used as heavy metal biosorben because of it’s adsorption capability that caused by the active cluster which contained in that species (Sembiring et al., 2008). Chlorella sp. is one of phytoplankton species that has the bioaccumulation ability of heavy metals and easily cultivated (Arifin, 1997). Lead (Pb) is a mineral belonging to microelements, is a heavy metal and is a potentially toxic material. Water bodies that have been polluted by compounds or ions Pb because can lead to the death of aquatic biota, the number of Lead (Pb) present in water bodies exceeding the proper concentration (Palar, 2004). One way to anticipate the increasing heavy metal pollution in the water is to bioremediation. The research using experimentally, the research design used was completely randomized design (CRD) consisting of four treatments with five replications. The concentrations of heavy metals Plumbum (Pb) used is 0 ppm and 0.9 ppm. The main parameters in this study is the ability of bioremediation Plumbum (Pb) by Nannochloropsis sp. and Chlorella sp. SPSS analytics normality test results and test the ability of T 95% in Nannochloropsis sp. and Chlorella sp. in absorbing heavy metals Plumbum (Pb) concentrations of 0 ppm and 0.9 ppm indicate that the data is normal and the results obtained were significantly different / significant. While the analysis of SPSS test T on heavy metal absorption capability comparison Plumbum (Pb) concentration of 0 ppm and 0.9 ppm by Nannochloropsis sp. and Chlorella sp. showed that the results were not significantly different / non significant. On average results obtained, Nannochloropsis sp. have a higher capacity than Chlorella sp. in the bioremediation process of heavy metals Plumbum (Pb).

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Evaluation of Phytoremediation Potential of Castor Cultivars for Heavy Metals from Soil

Planta Daninha ◽

10.1590/s0100-83582019370100134 ◽

2019 ◽

Vol 37 ◽

Author(s):

M.J. KHAN ◽

N. AHMED ◽

W. HASSAN ◽

T. SABA ◽

S. KHAN ◽

...

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Contaminated Soils ◽

Metal Uptake ◽

Plant Biomass ◽

Ricinus Communis ◽

Contaminated Sites ◽

Heavy Metal Uptake ◽

Randomized Design ◽

Completely Randomized Design

ABSTRACT: Phytoremediation is a useful tool to restore heavy metals contaminated soils. This study was carried out to test two castor (Ricinus communis) cultivars [Local and DS-30] for phytoextraction of heavy metals from the soil spiked by known concentrations of seven metals (Cu, Cr, Fe, Mn, Ni, Pb and Zn). A pot experiment was laid out by using a completely randomized design. Soil and plant samples were analyzed at 100 days after planting. The data on heavy metal uptake by plant tissues (roots, leaves and shoots) of the two castor cultivars suggested that a considerable amount of metals (Fe = 27.18 mg L-1; Cu = 5.06 mg L-1; Cr = 2.95 mg L-1; Mn = 0.22 mg L-1; Ni = 4.66 mg L-1; Pb = 3.33 mg L-1; Zn = 15.04 mg L-1) was accumulated in the plant biomass. The soil heavy metal content at the end of experiment significantly decreased with both cultivars, resulting in improved soil quality. Therefore, it is concluded that both castor cultivars, Local and DS-30, can be used for phytoremediation of heavy metal-contaminated sites.

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Pemanfaatan Arang Aktif Ampas Kopi Sebagai Adsorben Kadmium Pada Air Sumur (The Usage of Coffee Waste Actived Charcoal as Adsorbent of Cadmium in Well Water)

Jurnal Teknologi Lingkungan Lahan Basah ◽

10.26418/jtllb.v7i1.31115 ◽

2019 ◽

Vol 7 (1) ◽

pp. 011

Author(s):

Anita Dewi Moelyaningrum

Keyword(s):

Research Method ◽

Active Charcoal ◽

Well Water ◽

Test Results ◽

Coffee Waste ◽

Normality Test ◽

Randomized Design ◽

Coffee Ground ◽

Completely Randomized Design ◽

One Way Anova

Logam berat masih banyak ditemukan mencemari lingkungan. Cadmium adalah logam berat yang bisa ditemukan didalam air disekitar tempat pembuangan sampah. Penelitian ini bertujuan untuk menganalisis arang aktif ampas kopi dalam menurunkan kadar Cd dalam air. Metode penelitian ini adalah true eksperimental dengan desain Rancangan Acak Lengkap. Tahap pertama adalah pembuatan arang kemudian diaktivasi dengan HCl. Hasil Uji kualitas arang aktif ampas kopi meliputi rendemen (98,5%), kadar air (7%), kadar abu (4%) dan daya serap idoin (687,96 mg/g). Terdapat empat kelompok yaitu tanpa perlakuan 0 gr/l (P0), perlakuan dengan 5 gr/ l (P1), 8 gr/l (P2) dan 10g/l (P3), dimana masing-masing kelompok terdiri dari 6 replikasi. Kelompok yang diberi perlakuan dikontakkan dengan arang aktif ampas kopi selama 2 jam. Selanjutnya dilakukan analisa kadar Cd dalam air dengan metode Spektrofotometri Serapan Atom. Hasil penelitian dilakukan uji normalitas kemudian dilakukan uji one way anova menggunakan SPSS. Hasil menunjukkan terdapat perbedaan secara signifikan sebesar 0,000 dengan interval kepercayaan 95% antara kelompok kontrol dan perlakuan. Arang aktif ampas kopi secara significant dapat mengikat kadar cadmium dalam air. Heavy metal cadmium (Cd) is still polluted the environment. It can be found in the wells around the landfill. This study aimed to analyze the active charcoal coffee ground to adsorb the cadmium in the water. This research method is the true experimental design with Completely Randomized Design. The first stage is making charcoal and then activated with HCl. There are four groups 0 gram/littre (P0), perlakuan dengan 5 gram/littre (P1), 8 gram/littre (P2) dan 10 gram/littre (P3) with each group consisting of 6 replication. Treatement group contacted with active charcoal coffee around 2 hours. Results of research normality test later conducted ANOVA test. The test results informed that there is 0,000 difference significanly with 95% confidence intervals. The active charcoal coffee had significant to adsorb cadmium in the water.

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Pemanfaatan Arang Aktif Ampas Kopi sebagai Adsorben Kadmium pada Air Sumur

10.31227/osf.io/85ets ◽

2019 ◽

Author(s):

Anita Dewi Moelyaningrum

Keyword(s):

Research Method ◽

Active Charcoal ◽

Well Water ◽

Test Results ◽

Coffee Waste ◽

Normality Test ◽

Randomized Design ◽

Coffee Ground ◽

Completely Randomized Design ◽

One Way Anova

Jurnal Teknologi Lingkungan Lahan Basah, Vol. 02, No. 1, 2019: 011 - 019 Pemanfaatan Arang Aktif Ampas Kopi sebagai Adsorben Kadmium pada Air Sumur The Usage of Coffee Waste Actived Charcoal as Adsorbent of Cadmium in Well Water Puput Baryatik, Anita Dewi Moelyaningrum, Uswatun Asihta, Wita Nurcahyaningsih, Azzumrotul Baroroh, Herdian Riskianto. Bagian Kesehatan Lingkungan dan Kesehatan Keselamatan Kerja Fakultas Kesehatan Masyarakat Universitas Jember [email protected] Logam berat masih banyak ditemukan mencemari lingkungan. Cadmium (Cd) adalah logam berat yang bisa ditemukan di dalam air di sekitar tempat pembuangan sampah. Penelitian ini bertujuan untuk menganalisis arang aktif ampas kopi dalam menurunkan kadar Cd dalam air. Metode penelitian ini adalah true eksperimental dengan desain Rancangan Acak Lengkap. Tahap pertama adalah pembuatan arang kemudian diaktivasi dengan HCl. Hasil uji kualitas arang aktif ampas kopi meliputi rendemen (98,5%), kadar air (7%), kadar abu (4%) dan daya serap idoin (687,96 mg/g). Terdapat empat kelompok yaitu tanpa perlakuan 0 gr/l (P0), perlakuan dengan 5 gr/ l (P1), 8 gr/l (P2) dan 10g/l (P3), di mana masing-masing kelompok terdiri dari 6 replikasi. Kelompok yang diberi perlakuan dikontakkan dengan arang aktif ampas kopi selama 2 jam. Selanjutnya dilakukan analisa kadar Cd dalam air dengan metode Spektrofotometri Serapan Atom. Hasil penelitian dilakukan uji normalitas kemudian dilakukan uji one way anova menggunakan SPSS. Hasil menunjukkan terdapat perbedaan secara signifikan sebesar 0,000 dengan interval kepercayaan 95% antara kelompok kontrol dan perlakuan. Arang aktif ampas kopi secara signifikan dapat mengikat kadar kadmium dalam air. Heavy metal cadmium (Cd) is still polluted the environment. It can be found in the wells around the landfill. This study aimed to analyze the active charcoal coffee ground to adsorb the cadmium in the water. This research method is the true experimental design with Completely Randomized Design. The first stage is making charcoal and then activated with HCl. There are four groups 0 gram/littre (P0), perlakuan dengan 5 gram/littre (P1), 8 gram/littre (P2) dan 10 gram/littre (P3) with each group consisting of 6 replication. Treatement group contacted with active charcoal coffee around 2 hours. Results of research normality test later conducted ANOVA test. The test results informed that there is 0,000 difference significanly with 95% confidence intervals. The active charcoal coffee had significant to adsorb cadmium in the water.

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Korelasi Antara Daya Serap Gracilaria sp. Terhadap Konsentrasi Logam Berat Cu di Media Pemeliharaan

Journal of Marine Research ◽

10.14710/jmr.v10i3.30732 ◽

2021 ◽

Vol 10 (3) ◽

pp. 363-368

Author(s):

Regina Amanda ◽

Ria Azizah Tri Nuraini ◽

Endang Supriyantini

Keyword(s):

Water Pollution ◽

Heavy Metals ◽

Heavy Metal ◽

Weight Gain ◽

Metal Content ◽

Absorption Capacity ◽

Control Treatment ◽

Living Things ◽

Randomized Design ◽

Completely Randomized Design

Pencemaran air terhadap lingkungan dapat menyebabkan dampak membahayakan yang dapat dirasakan oleh makhluk hidup. Salah satu logam berat yang sering mencemari air adalah logam berat tembaga (Cu). Tujuan penelitian ini untuk mengetahui kemampuan daya serap rumput laut Gracilaria sp. terhadap logam berat Cu dan pertumbuhannya. Penelitian ini menggunakan metode eksperimental laboratoris dengan Rancangan Acak Lengkap (RAL) dengan menggunakan 4 perlakuan penambahan konsentrasi Cu, yaitu kontrol (0,54 ppm, sesuai dengan konsentrasi Cu pada air laut), 3 ppm, 6 ppm, 9 ppm dengan 3 kali pengulangan. Pengukuran kandungan logam Cu terhadap Gracilaria sp. dengan menggunakan Atomic Absorpsion Spektofotometri (AAS). Hasil penelitian menunjukkan bahwa penyerapan logam berat Cu tertinggi terdapat pada konsentrasi 9 ppm, yaitu sebesar 3,63 ppm dan terendah pada kontrol yaitu sebesar 0,27 ppm.. Pertumbuhan Gracilaria sp. tertinggi terdapat pada perlakuan kontrol dengan pertambahan berat sebesar 209,2 gram dan terendah pada konsentrasi 9 ppm, yaitu sebesar 162,9 gram. Semakin tinggi konsentrasi Cu yang diberikan maka semakin rendah pertumbuhan Gracilaria sp. Water pollution to the environment can cause dangerous impacts that can be felt by living things. One of the heavy metals that often pollutes water is the heavy metal copper (Cu). The purpose of this study was to determine the absorption capacity of Gracilaria sp. to the heavy metal Cu and its growth. This study used a laboratory experimental method with a completely randomized design (RAL) using 4 additional treatments of Cu concentration, namely control (0.54 ppm, according to the Cu concentration in seawater), 3 ppm, 6 ppm, 9 ppm with 3 repetitions. Measurement of Cu metal content against Gracilaria sp. by using Atomic Absorption Spectophotometry (AAS). The results showed that the highest absorption of heavy metal Cu was at a concentration of 9 ppm, which was 3.63 ppm and the lowest was 0,27 ppm in control.. The Growth of Gracilaria sp. The highest was found in the control treatment with a weight gain of 209.2 grams and the lowest was at a concentration of 9 ppm, which was 162.9 grams. The higher the concentration of Cu given, the lower the growth of Gracilaria sp.

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PENGGUNAAN COAL FLY ASH (CFA) DAN TANDAN KOSONG KELAPA SAWIT (TKKS) UNTUK PERBAIKAN TANAH ULTISOL DAN SERAPAN LOGAM BERAT

BUANA SAINS ◽

10.33366/bs.v20i1.1928 ◽

2020 ◽

Vol 20 (1) ◽

pp. 21-28

Author(s):

Aryo Candra Nugroho ◽

Amir Hamzah ◽

Hesti Triana Soelistriari

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Fly Ash ◽

Metal Content ◽

Main Type ◽

Coal Fly Ash ◽

Land Area ◽

Randomized Design ◽

Completely Randomized Design ◽

Very High

Ultisol is the main type of land in Kalimantan, Sumatra, Papua and Sulawesi, with an area of almost 45.8 million ha or 25% of the land area of Indonesia. Ultisol has a relatively high acidity (average pH 4.5), high Al saturation, reaching 60%. The efforts to increase the fertility of ultisol soils such as the addition of organic materials such as Empty Oil Palm Bunches (OPEFB) and CFA (Coal Fly Ash) or Coal Fly Ash (CFA) improve the physical properties of Ultisol soil. Use of CFA that exceeds the limit can cause pollution especially copper (Cu) and lead (Pb). One way to recover soil from heavy metal contaminants with Phytoremediation technology, mustard (Brassica juncea L.) is one of the plants that easily absorb heavy metals so that it can be used as a Phytoremediation agent. The aims of this research to determine the use of CFA and OPEFB to fix up the ultisol soil and heavy metal content absorbed by plants. This study used Completely Randomized Design with 3 replications. The results showed that using of CFA and OPEFB can increase the available P so that it can increase the growth of mustard plants and absorption of heavy metals. By using the dozes of 54.54g CFA and 18.18g OPEFB (C3K1) can increase P- available at 181.67 m kg-1 . The uptake of heavy metals Cu and Pb in mustard plants is very high. The highest uptake in Cu reached 192.03 mg kg-1 , whereas in Pb it was 80 mg kg-1 . This number is still very high, so it is dangerous if consumed.

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The Growth and Antioxidative Responses of Sonchus oleraceus (Linnaeus, 1753) Under Cu(II), Pb(II), Cd(II) and Cr(VI) Stress Condition

KnE Life Sciences ◽

10.18502/kls.v3i4.700 ◽

2017 ◽

Vol 3 (4) ◽

pp. 157

Author(s):

Nur Hidayaturrohmah ◽

Sri Kasmiyati ◽

Sucahyo . ◽

Zane Vincēviča-Gaile

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Dry Weight ◽

Sonchus Oleraceus ◽

Antioxidative Responses ◽

Randomized Design ◽

Growing Medium ◽

Metal Treatment ◽

Completely Randomized Design ◽

The Impact

Plants growing in soil containing heavy metal polutan such as chromium (Cr), lead (Pb), cadmium (Cd) and copper (Cu) will be stunted, and increase production of Reactive Oxygen Species (ROS). In dealing with the excess amount of ROS, plants have an enzymatic defense system, using superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX). The aim of this study was to determine and analyze the Sonchus oleraceus (Linnaeus, 1753) plant response to heavy metals stress, seen from the growth and antioxidative defense enzymatically. Research carried out using a Completely Randomized Design (CRD) with four treatments and five replicates. The metal treatment was 10 mg · L–1. The presence of heavy metals in the growing medium significantly decreased the plant height and leaf area, so the impact is on the weight of wet and dry weight. The metal treatments of Cr, Cd, Pb and Cu increase the activity of SOD and APX enzymes but decrease the activity of the CAT enzyme. Chromium is a metal that has a significant influence on the growth and activity of SOD, APX, and CAT enzymes in S. oleraceus.<div>Keywords: antioxidative responses; growth; heavy metal; Sonchus oleraceus; toxicity.</div>

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Efektivitas Air Rendaman Jerami Alang-Alang (Imperata cylindrica) sebagai Atraktan terhadap Jumlah Telur Aedes aegypti

BALABA JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA ◽

10.22435/blb.v16i1.2789 ◽

2020 ◽

pp. 39-46

Author(s):

Budi Hairani ◽

Muhammad Rasyid Ridha ◽

Abdullah Fadilly ◽

Gusti Meliyanie ◽

Akhmad Rosanji

Keyword(s):

Aedes Aegypti ◽

Imperata Cylindrica ◽

Test Results ◽

Anova Test ◽

Normality Test ◽

Randomized Design ◽

Completely Randomized Design ◽

Gravid Mosquitoes ◽

Development Center ◽

Concentration Levels

The use of ovitrap with attractants modification has been known as an alternative method in controlling DHF vectors. Attractant material will be better if it uses natural ingredients that are cheap and easy to obtain and easy to manufacture. Attractant material is recommended to use natural ingredients that are cheap and easy to obtain and are easy to make. Imperata plants can be easily obtained and easily processed as attractant material. This study aims to determine the effectiveness of attractants on a laboratory scale in the form of immersion water from the straw of the Imperata cylindrica plants in various concentration levels to Aedes aegypti eggs number. Research with an experimental design using a completely Randomized Design (CRD) was conducted at the Entomology Laboratory of the Tanah Bumbu Research and Development Center, in July 2019. Treatment concentrations consisted of 5%, 10%, 15%, and 20%, control using akuades. Samples for testing using Ae. aegypti gravid mosquitoes. Treatment with 5 repetitions, carried out for 7 days. Data analyzed by normality test, Anova test, and Least Significant Differences (LSD) test. The average number of eggs at concentrations of 5%, 10%, 15%, and 20% respectively was 45.00; 78.60; 129.40; and 174.00. Anova test showed Imperata cylindrica straw soaking attractant had a significant effect on oviposition of Ae. aegypti mosquitoes. LSD test results showed attractant 15% is the optimal concentration in increasing the number of eggs in the ovitrap.

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POTENSI PENURUNAN KONSENTRASI LOGAM BERAT TEMBAGA (CU) DAN PERTUMBUHAN MIKROALGA SPIRULINA PLANTESIS PADA MEDIA KULTUR

Jurnal Akuakultur Rawa Indonesia ◽

10.36706/jari.v6i1.7152 ◽

2018 ◽

Vol 6 (1) ◽

pp. 83-93

Author(s):

Mochamad Rendy Sety0 Budi ◽

Boedi Setya Rahardja ◽

Endang Dewi Masithah

Keyword(s):

Water Pollution ◽

Heavy Metals ◽

Heavy Metal ◽

Experimental Method ◽

Spirulina Platensis ◽

Living Things ◽

Randomized Design ◽

Environmental Component ◽

Completely Randomized Design ◽

Bioremediation Agent

ABSTRACT Water is an important environmental component for life. Heavy metal water pollution comes from many industries. Heavy Metals Copper (Cu) is one of several other heavy metals that are harmful to living things. One way to anticipate the increased pollution of heavy metals Copper (Cu) in waters is bioremediation using microalgae. This study aims to determine the ability of Spirulina plantesis in absorbing heavy metals Copper (Cu) and to determine the influence of heavy metal Copper (Cu) on the growth of Spirulina platensis. This study used an experimental method with Completely Randomized Design (RAL) consisting of four treatments and five replicates, namely A (S. platensis 0 ppm), B (S. platensis 1 ppm), C (S. platensis 3 ppm), D (S. Platensis 5 ppm). The results showed that Spirulina platensis was able to absorb heavy metal of Copper (Cu) so that it can be used as a heavy metal bioremediation agent. On treatment B (1 ppm) absorption of 87,719%, C (3 ppm) equal to 97,886% and D (5ppm) equal to 95,872 % Growth with the addition of Cu affects Spirulina platensis growthKeywords: Bioremediation, Spirulina platensis, Copper, Growth

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BIOREMEDIASI Pb OLEH BAKTERI INDIGEN LIMBAH CAIR AGAR

Jurnal Biosilampari Jurnal Biologi ◽

10.31540/biosilampari.v1i2.288 ◽

2019 ◽

Vol 1 (2) ◽

pp. 51-58

Author(s):

Senja Ikerismawati

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Research Design ◽

Bacillus Pumilus ◽

Liquid Waste ◽

Bacterial Isolates ◽

Indigenous Bacteria ◽

Randomized Design ◽

Treatment Data ◽

Completely Randomized Design

Bacteria isolated from ad environment contaminated with heavy metals are very potential as heavy metal bioremediation agents called indigenous bacteria. The purpose of this study was to isolate and identify indigenic bacteria that have the potential as Pb bioremediation agents in agar liquid waste and to analyze the ability of indigenic bacteria in reducing Pb. The research design used Completely Randomized Design (RAL) two factorial with variation of bacteria and eight days of treatment. Data were analyzed using two-way ANOVA with Duncan's advanced test. The results showed that there were eight isolates resulting from the isolation of agar liquid waste. The isolates of liquid waste indigen bacteria so that the most potential in reducing Pb were isolated H, E and F. The three isolates were able to reduce Pb in sterile agar liquid waste by 82.6%, 81.3% and 79.3% for eight days of treatment. The identification results using Microbact TM GNB 12A / B / E, 24 Identification Kits showed that H bacterial isolates were Bacillus alvei, E isolates were Bacillus pumilus species and F isolates were Bacillus lichenformis species

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Use of Chlorella sp. for coating ‘tommy atkins’ mango fruits stored under refrigeration

Semina Ciências Agrárias ◽

10.5433/1679-0359.2018v39n2p565 ◽

2018 ◽

Vol 39 (2) ◽

pp. 565 ◽

Cited By ~ 1

Author(s):

Agda Malany Forte de Oliveira ◽

Railene Hérica Carlos Rocha ◽

Wellinghton Alves Guedes ◽

George Alves Dias ◽

José Franciraldo de Lima

Keyword(s):

Relative Humidity ◽

Raw Materials ◽

Storage Period ◽

Titratable Acidity ◽

Soluble Solids ◽

Edible Coatings ◽

Fruit Maturation ◽

Chlorella Sp ◽

Randomized Design ◽

Completely Randomized Design

The number of studies on edible coatings that are used for extending the shelf life of fruits has steadily increased. For this purpose, it is necessary to choose raw materials with characteristics that maintain product quality. The objective of this study is to evaluate the effect of coatings of Chlorella sp. on the post-harvest conservation of ‘Tommy Atkins’ mango fruits. The experiment was conducted using a completely randomized design, and spray treatments (T) with 0%, 1%, 2%, 3%, or 4% of Chlorella sp. were applied to the fruits. After the treatments, the fruits were stored for 28 days (21 days at 10 °C and 42% relative humidity and 7 days at 25 °C and 42% relative humidity), and were analyzed on day 28. There was higher pulp firmness in fruits as the concentration of Chlorella sp. was increased, and the concentration of ascorbic acid was comparatively higher (22.17%) in fruits treated with 3% Chlorella sp. The percentage of soluble solids and the soluble solids/titratable acidity ratio were decreased as the coating concentrations were decreased. The use of Chlorella sp. at 1% and 2% allowed fruit maturation without compromising fruit quality attributes during the 28-day storage period.

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