Rice (Orysa sativa L.) seed storage under controlled conditions

Author(s):  
OA Zadorozhna ◽  
TP Shyianova ◽  
MYu Skorokhodov ◽  
TM Shpak

Viability of rice (Orysa sativa L.) seeds accessions: subsp. japonica: var. italica, var. nigro-apiculata, var. subvulgaris; subsp. indica, var. mutica and var. fortuna was analised. The seed accessions were grown in the southern steppe of Ukraine and then stored at 4°C and −20°C. According to the research results, it is established that rice seeds in favorable cultivation years are able to maintain the original germination without significant changes for 10 years and longer with 6-8% moisture content even at 4°C storage temperature. There were no differences of rice seeds longevity of the same accessions stored at 4°C and −20°C. Differences of rice seed longevity of subsp. japonica and subsp. indica are discussed.

Author(s):  
O.A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov

Aim. The aim of this work was to determine the longevity of durum wheat seeds according to the results of seed germination monitoring after seed storage for up to 19 years under controlled conditions in the National Plant Gene Bank of Ukraine. Results and Discussion. 36 accessions of spring durum wheat were investigated. These accessions belonged to var. hordeiforme, var. leucurum, var. melanopus, var. alexandrinum, var. apulicum, var. australe from seven countries; seven samples of durum winter wheat belonged to var. hordeiforme, var. leucurum were from Ukraine. Accessions were received by the Ukrainian genebank from seven countries: Ukraine, Russia, Mexico, France, Portugal, Kazakhstan and Tunisia. Seed accessions for storage were grown mainly in the eastern forest-steppe of Ukraine, stored in the National depositary in this region at unregulated temperature and at 4°C with seed moisture content of 5.5-8.0%. The mode of seed drying, which took place at temperature not higher than 25°C is discussed. The obtained results indicate high seed longevity of durum wheat under these conditions with initial seed germination rate more than 90% even in a storage facility at unregulated temperature. There were no differences in seed longevity between varieties of durum wheat under the studied storage conditions. Conclusions. Seeds of durum wheat remain unchanged for at least 10 years with a high initial germination of seeds, storage in sealed containers with moisture content 5.5-8.0% even at unregulated temperature of the eastern forest-steppe of Ukraine. Durum wheat seeds should be storage at temperature 4°C to increase seed longevity at seed moisture 5.5-8.0%.


HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 782-785 ◽  
Author(s):  
Wayne A. Mackay

Seeds of Lupinus havardii Wats. and L. texensis Hook. were subjected to scarification, storage temperature (4 or 22 °C), and relative humidity (RH) treatments (11%, 23%, 52%, 75%, or 97% RH) for 12 months. Seed moisture increased as relative humidity increased with scarified seed having the greatest increase in seed moisture content regardless of storage temperature. For both species, the combination of seed scarification before storage, 75% RH, and 22 °C storage temperature resulted in a significant and rapid decline in germinability beginning at 4 months. Scarified L. texensis seed stored at 52% RH and 22 °C also exhibited a significant decline in germinability following 6 months storage. Seed of both species stored under all other conditions germinated similar to or higher than the initial germination rate after 12 months. These results clearly show that scarification can be performed before seed packaging as long as the seed packets are stored at ≤23% RH under 4 or 22 °C with no loss in germinability for at least 1 year.


2019 ◽  
Vol 7 (2) ◽  
pp. 228
Author(s):  
I Putu Mas Pradnyana Wibawa ◽  
Ida Bagus Putu Gunadnya ◽  
I Made Anom Sutrisna Wijaya

Tujuan dari dilakukanya penelitian Ini adalah untuk menduga umur simpan benih padi menggunakan metode ASLT (Accelerrated Shelf Life Testing) dengan pendekatan model kadar air kritis. Adapun benih yang digunakan adalah benih varietas Ciherang dan varietas Inpari 30. Kemasan dari benih padi adalah plastic jenis Polipropilen (PP). Benih padi disimpan pada kondisi lingkungan (suhu 290C dan RH 75%). Pengamatan benih dianggap kritis ketika persentase daya berkecambah di bawah 80%. Ditemukan dari hasil pengukuran bahwa nilai permeabilitas kemasan (k/x) dari plastic PP adalah 0,013 gH2O/m2.day.mmHg. Pengamtan kadar air awal (Ma) adalah 13 %, kadar air kritis (Mc) adalah 29 %, total padatanya (WS) adalah 8,8 kg, dan perbedaan tekanan (?P) 1,15 mmHg. Sementara Inpari 30 memiliki nilai kadar air awal (Ma) 14%, kadar air kritis 29%, total padatannya (WS) 8,7 kg, dan ?P 0,9 mmHg.  Dari nilai tersebut dapat diduga umur simpan dua varietas benih bersertifikat yang dikemas dalam plastik PP dan disimpan pada suhu 29oC dan RH 75% adalah 156 hari (5,03 bulan) untuk varietas Ciherang dan 254 hari (8,19 bulan)  untuk 30 varietas Inpari.   The purpose of this study was to prediction the shelf life of certified rice seeds using the ASLT (Accelerated Shelf Life Testing) method based on the critical moisture content approach. The varieties of rice seeds used in this research were Ciherang and Inpari 30 varieties which packed with polypropylene (PP) plastic packaging. The rice seeds packages were stored at environmental condition (temperature of 29oC and RH of 75%). Observation on seeds germination was done daily until the seeds have a germination percentage below 80%. When the seeds under this condition, moisture content of the seeds was measured and refer as critical moisture content. It was found that the packaging permeability (k/x) of PP plastic was 0.013 g H2O/m2.day.mmHg. Observation on rice seed of Ciherang variety revealed that its values of initial moisture content (Ma) was 0.133 %, its critical moisture content (Mc) was 0.291 %, its total solid (Ws) was 8.8 kg, and pressure difference  (?P) was 1,15 mmHg. While, for Invary 30 variety the values of its shelf life parameters were as follows: Ma 0.144 %, Mc 0.293 %, Ws 8.7 kg, and ?P 0.9 mmHg. The prediction of shelf life the two certified rice seeds which were packed in PP plastic and stored at 29oC and RH of 75% were 156 days (5.03 months) for Ciherang variety and 254 days (8.19 months). ) for Inpari 30 variety.


2017 ◽  
Vol 13 (11) ◽  
Author(s):  
Pan Wang ◽  
Nan Fu ◽  
Dong Li ◽  
Li-jun Wang

AbstractThe desorption isotherms ofjaponicaandindicarice seeds were determined at 15, 25, 35, 45 °C within the water activity range from 0.12 to 0.92 and it was observed that GAB model could accurately describe the desorption behaviors with low values of mean relative error (MRE). The thermodynamic properties of rice seeds were obtained by water desorption isotherms. The moisture content with the minimum integral entropy was considered as the best storage condition where the strongest energetic interaction between water molecular and solid occurred and the optimum seed moisture for storage at 35 °C occurred at 6.02 g water per 100 g of dry matter (ɑwof 0.12) forjaponicarice seed and 5.28 g water per 100 g of dry matter (ɑwof 0.08) forindicarice seed, respectively. The mechanisms of desorption process were determined with integral enthalpy–entropy compensation theory and it was observed that the at low moisture content the desorption process of rice seed was controlled by changes of entropy, whereas the desorption process was considered to be controlled by enthalpy mechanism at high moisture content. It was found that theindicarice seed exhibited a wider water activity range in the minimum integral entropy zone, indicating thatindicarice seed was more stable to withstand the different storage conditions compared withjaponicarice seed.


1991 ◽  
Vol 1 (1) ◽  
pp. 69-72 ◽  
Author(s):  
R. H. Ellis ◽  
T. D. Hong ◽  
E. H. Roberts

AbstractSeeds of papaya (Carica papaya L.) stored for 12 months at 15°C with 7.9–9.4% moisture content maintained their original germination. In contrast, many seeds stored cooler or drier lost viability, the losses occurring more rapidly at −20°C than at either 0°C or 15°C. The results are not compatible with the definitions of either orthodox or recalcitrant seed storage behaviour.


1993 ◽  
Vol 3 (3) ◽  
pp. 201-213 ◽  
Author(s):  
Christina W. Vertucci ◽  
Eric E. Roos

AbstractThe premise of this paper is that the chemical potential of water strongly influences aging reactions in seeds, and that there will be an optimal chemical potential of water for seed longevity. If this is true, the optimum moisture content for storage and the optimal drying protocols should vary with the storage temperature, but should be predictable from water sorption isotherms. Isotherms for pea, soybean and peanut are given for temperatures between 65° and −150°C. Relative humidity/moisture content relationships were determined directly for temperatures between 5° and 50°C using saturated salt solutions. At extreme temperatures, isotherms were calculated from heat capacity measurements using differential scanning calorimetry or extrapolations of van't Hoff analyses. The family of isotherms was used to predict optimum moisture contents at storage temperatures between 65°C and −150°C. The optimal moisture contents for these three species are consistently shown to increase as the storage temperature is lowered.


2018 ◽  
Vol 28 (4) ◽  
pp. 332-339 ◽  
Author(s):  
K.J. Whitehouse ◽  
F.R. Hay ◽  
R.H. Ellis

AbstractDrying reduces seed moisture content, which improves subsequent seed survival periods. Diverse maximum temperatures have been recommended to limit or avoid damage to seeds, but some high-temperature drying regimes may improve subsequent seed quality. Seeds from 20 different accessions of five rice (Oryza sativaL.) variety groups (aromatic, Aus, Indica, temperate Japonica, tropical Japonica) were harvested over several seasons at different stages of maturation and either dried throughout at 15°C/15% relative humidity (RH) or for different initial periods (continuous or intermittent) in different drying regimes at 45°C before final equilibrium drying at 15°C/15% RH. Subsequent seed longevity in hermetic storage at 45°C with 10.9% moisture content was determined. In no case did initial drying at 45°C provide poorer longevity than drying at 15°C/15% RH throughout. There was a split-line relation, which did not differ amongst investigations, between longevity after initial drying at 45°C relative to that at 15°C/15% RH throughout and harvest moisture content, with a break point at 16.5% (a seed moisture status of about –14 MPa). Below 16.5%, relative longevity did not differ with harvest moisture content with little or no advantage to longevity from drying at 45°C. Above 16.5%, relative longevity showed a positive relation with harvest moisture content, with substantial benefit from drying at 45°C to subsequent longevity of seeds harvested whilst still moist. Hence, there are temporal (immediatelyex plantacf. subsequent air-dried storage) and water status discontinuities (above cf. below 16.5%) in the effect of temperature on subsequent air-dried seed longevity.


1999 ◽  
Vol 39 (8) ◽  
pp. 1025 ◽  
Author(s):  
Yin Yanping ◽  
Gao Rongqi ◽  
Sun Qingquan ◽  
Li Shengfu

Summary. The vigour of Welsh onion (Allium fistulosum L.) seeds with low moisture content was determined after exposure to different storage temperatures and container types. As the storage period was prolonged, the effects of storage temperature and container type on seed vigour became more pronounced. Storage temperature had a greater effect on seed vigour than container types, and the effect of the latter was subject to the storage temperature. When the seeds were stored in the same type of container, several indices of the seed vigour declined as the storage temperature increased. A highly significant positive correlation was evident between dehydrogenase activity and the percentage germination of the seed. Improving storage conditions could prolong seed longevity. If the seeds, in which the moisture content has been reduced to less than 6%, are stored airtight, the percentage germination will be about 85% after storage for 2 years at room temperature or after 3-year storage at 6°C. If the seeds are stored at –6°C, the percentage germination will range from 86 to 91% after 3-year storage, regardless of the storage container types in this study. These data are very important for the seed storage and germplasm conservation of Welsh onion.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 490c-490
Author(s):  
Jian Fang ◽  
Frank Moore ◽  
Eric E. Roos ◽  
Christina Walters

Seed moisture content (MC) changes depending upon relative humidity (RH) and temperature (T). This relationship was revealed by studying the interaction of RH and T at equilibrium. Maize, cucumber, onion, lettuce, watermelon, and pea seeds were equilibrated over various saturated salt solutions (1% to 93% RH) at temperatures from 5 to 50 °C. Five-hundred-eleven subset models were selected from a complete third-order model MC = 0 + 1*RH + 2*T + 3*RH2 + 4*T2 + 5*RH*T + 6*RH3 + 7*T3 + 8*RH*T2 + 9*RH2*T using Mallows' minimum Cp as the selection criterion. All six best subset models were found to have the same functional form, MC0 + 1 = *RH + 2*T + *RH2 + 5*RH*T + 6*RH + 9*RH2*T. Coefficients had essentially the same respective values among species, except for the onion and pea models (P ≤ 0.05). All models indicated that seed MC increased as RH increased and decreased as temperature increased. A previous paper (Vertucci and Roos, 1990) indicated that optimum RH for orthodox seed storage is between 19% and 27%. Using these models, the range for the optimum moisture content for storage of most orthodox seeds can be quickly calculated for any given storage temperature.


1993 ◽  
Vol 29 (4) ◽  
pp. 449-459 ◽  
Author(s):  
G. N. Zanakis ◽  
R. H. Ellis ◽  
R. J. Summerfield

SummaryReports that an Indonesian soyabean (Glycine max) line (TGm737p) shows greater seed storage longevity than an American cultivar (Bossier), and that the cross between them (TG×536–02D) is intermediate between the two parents in this regard, were investigated by growing all three genotypes under identical conditions (30°C/20°C, 12 h d-1 photoperiod) in a plastics house. Seeds were harvested when moisture content had declined naturally to 14–15% (wet basis), and then stored hermetically at five different moisture contents at 40°C. Analyses of the resultant seed survival curves revealed that the seed lot constant Ki (a measure of potential longevity) was greatest in TGm737p and least in cv. Bossier, the cross being much closer to the American parent in this regard. The negative logarithmic relation between σ (standard deviation of the frequency distribution of seed deaths in time) and moisture content did not differ (p > 0.10) among the three genotypes. Moreover, this relation was close to that predicted by earlier results for four different genotypes. Similarly, there was no significant difference (p > 0.25) among genotypes in the negative semi-logarithmic relation between σ and seed equilibrium relative humidity (rh): the regression slope was equivalent to a doubling of longevity for each 8.0% reduction in rh. Finally, comparison of the negative logarithmic relation between absolute longevity (50% viability period) and seed moisture content confirmed that TGm737p showed greatest longevity (p > 0.005), but failed to show a difference in absolute longevity between cv. Bossier and the cross (p > 0.25). The results confirm that the seed viability equation of Ellis and Roberts (1980a, b) provides a framework within which the seed longevity of different genotypes can be compared. They suggest too that there is considerable doubt as to whether or not the greater potential longevity of the Indonesian line TGm737p has been incorporated successfully into the cross TG×536–02D.Longevidad de la semilla de soja


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