scholarly journals Polyphenolic profiles in edible Annona spp. using high-performance liquid chromatography (HPLC-MS/MS)

2020 ◽  
Vol 21 (3) ◽  
pp. 201-208
Author(s):  
Manjunath J Shetty ◽  
K. R Vasudeva ◽  
T Sakthivel ◽  
G. J Suresh ◽  
H. C Krishna ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Sinapic Acid ◽  
Syringic Acid ◽  
Polyphenolic Compounds ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid ◽  
Dihydroxybenzoic Acid ◽  
Normal Functioning ◽  
Polyphenolic Composition

Polyphenolic compounds of fruits of various plant species play an important role in physiological functions related to human health. Polyphenols have important biological activities like antioxidant activity which helps in normal functioning of human body. The objective of this study was to investigate the profiles of polyphenolic compounds in different edible Annona spp. fruits. Total of eighteen identified individual phenolic compounds were found, among which p-coumaric acid (321.53-90.17µg g-1FW), o-coumaric acid (70.80-19.00µg g-1FW), 2,4-dihydroxybenzoic acid (39.49-10.43µg g-1FW), caffeic acid (35.26-3.43µg g-1FW), gentisic acid (24.69-10.46µg g-1FW), protocatechuic acid (17.04-4.23µg g-1FW), t-cinnamic acid (22.68-3.93µg g-1FW) and ferulic acid (21.78-3.43µg g-1FW) were abundant in annona fruits while benzoic acid (23.28-4.61µg g-1FW), p-hydroxybenzoic acid (1.79-0.31µg g-1FW), salicylic acid (6.00-2.40µg g-1FW), 3-hydroxybenzoic acid (6.05-0.88µg g-1FW), vanillic acid.  (19.13-2.16µg g-1FW), gallic acid (15.88-2.74µg g-1FW), ellagic acid (1.12-0.20µg g-1FW), syringic acid (0.78-0.34µg g-1FW) and sinapic acid (2.16-0.79µg g-1FW) were limited. However, chlorogenic acid was not detected. The results obtained in this study will furnish a better knowledge of the polyphenolic composition in annona fruits.

Polyphenolic composition of grape stems

2020 ◽  
Vol 48 (3) ◽  
pp. 1543-1559
Author(s):  
Bozena PRUSOVA ◽  
Josef LICEK ◽  
Michal KUMSTA ◽  
Mojmir BARON ◽  
Jiri SOCHOR
Keyword(s):  
Antioxidant Activity ◽  
Protocatechuic Acid ◽  
Activity Concentration ◽  
Ammonia Nitrogen ◽  
Syringic Acid ◽  
Polyphenolic Compounds ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid ◽  
Total Polyphenols ◽  
Polyphenolic Composition

This study is focused on the study of polyphenolic compounds in grape stems as by-product of winemaking industry. Two white varieties of Grüner Veltliner and Sauvignon and two red varieties of Blauer Portugieser and Cabernet Moravia were selected for the study. Antioxidant activity, concentration of total polyphenols and concentration of individual phenolic compounds were determined. The results show a higher concentration of polyphenols and higher values of antioxidant activity in red varieties. The Blauer Portugieser variety contained the highest concentrations of syringic acid 1.346 mg.L-1, caffeic acid 20 mg.L-1, ferulic acid 1.192 mg.L-1, coumaric acid 3.231 mg.L-1, trans-resveratrol 14.195 mg.L-1, catechin 79.314 mg.L-1 and epicatechin 33.205 mg.L-1. Cabernet Moravia contained the highest concentration of protocatechuic acid 1.201 mg.L-1, the Sauvignon variety reached the highest concentration of gallic acid 4.015 mg.L-1 and hydroxybenzoic acid 0.076 mg.L-1. The highest values of alpha-amino acids were determined in the Blauer Portugieser variety 165.3 mg L-1 and the lowest in the Grüner Veltliner variety 33.3 mg L-1. The highest concentration of ammonia nitrogen was 214 mg L-1 for the Blauer Portugieser variety and the lowest concentration of ammonia nitrogen was measured in Cabernet Moravia 35.7 mg L-1.

PHENOLIC COMPOUNDS IN FERNS: I. A SURVEY OF SOME FERNS FOR CINNAMIC ACID AND BENZOIC ACID DERIVATIVES

1967 ◽  
Vol 45 (5) ◽  
pp. 585-593 ◽  
Author(s):  
Bruce A. Bohm ◽  
Rolla M. Tryon
Keyword(s):  
Phenolic Compounds ◽  
Protocatechuic Acid ◽  
Sinapic Acid ◽  
Syringic Acid ◽  
Hydroxybenzoic Acid ◽  
Benzoic Acids ◽  
Coumaric Acid ◽  
Cinnamic Acids ◽  
Benzoic Acid Derivatives ◽  
Dennstaedtia Punctilobula

Forty-six ferns, representing 28 genera and 8 families, were examined for hydroxylated cinnamic acids and benzoic acids. In most plants examined the "basic complement" of cinnamic acids, p-coumaric, caffeic, and ferulic, was present. In these plants p-hydroxybenzoic acid, protocatechuic acid, and vanillic acid were generally also present. Of much more limited distribution were sinapic acid, syringic acid, and o-coumaric acid. Coumarin was shown to be present in Dennstaedtia punctilobula, the only fern tested which possessed this type of compound. The data available from three ferns reported in the literature are also included in this survey; one represents an additional family and one represents an additional genus.An experiment with DL-phenylalanine-1-14C and DL-phenylalanine-3-14C and Cyathea arborea showed the incorporation of label into p-coumaric acid, caffeic acid, and ferulic acid.

scholarly journals Optimization of Plant Extract Purification Procedure for Rapid Screening Analysis of Sixteen Phenolics by Liquid Chromatography

Separations ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 13
Author(s):  
Petra Ranušová ◽  
Ildikó Matušíková ◽  
Peter Nemeček
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Solid Phase ◽  
Low Cost ◽  
Sinapic Acid ◽  
Rapid Screening ◽  
Coumaric Acid ◽  
Laboratory Equipment ◽  
Wide Range ◽  
Methoxycinnamic Acid

A solid-phase extraction (SPE) procedure was developed for simultaneous monitoring of sixteen different phenolics of various polarity, quantified by high-performance liquid chromatography (HPLC). The procedure allowed screening the accumulation of intermediates in different metabolic pathways that play a crucial role in plant physiology and/or are beneficial for human health. Metabolites mostly involved in phenylpropanoid, shikimate, and polyketide pathways comprise chlorogenic acid, gentisic acid, vanillic acid, caffeic acid, protocatechuic acid, ferulic acid, rutin, quercetin, epicatechin, gallic acid, sinapic acid, p-coumaric acid, o-coumaric acid, vanillin; two rarely quantified metabolites, 2,5-dimethoxybenzoic acid and 4-methoxycinnamic acid, were included as well. The procedure offered low cost, good overall efficiency, and applicability in laboratories with standard laboratory equipment. SPE recoveries were up to 99.8% at various concentration levels. The method allowed for routine analysis of compounds with a wide range of polarity within a single run, while its applicability was demonstrated for various model plant species (tobacco, wheat, and soybean), as well as different tissue types (shoots and roots).

scholarly journals Co-Ingestion of Natal Plums (Carissa macrocarpa) and Marula Nuts (Sclerocarya birrea) in a Snack Bar and Its Effect on Phenolic Compounds and Bioactivities

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 310
Author(s):  
Vimbainashe E. Manhivi ◽  
Retha M. Slabbert ◽  
Dharini Sivakumar
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Syringic Acid ◽  
Fruit Pulp ◽  
Coumaric Acid ◽  
Flight Mass Spectrometry ◽  
Sclerocarya Birrea ◽  
Internal Phase ◽  
Tof Ms

This study investigated the effect of co-ingesting Natal plums (Carissa macrocarpa) and Marula nuts (Sclerocarya birrea) on the bioaccessibility and uptake of anthocyanins, antioxidant capacity, and the ability to inhibit α-glucosidase. A Natal plum–Marula nut bar was made by mixing the raw nuts and the fruit pulp in a ratio 1:1 (v/v). The cyanidin-3-O-sambubioside (Cy-3-Sa) and cyanidin-3-O-glucoside content (Cy-3-G) were quantified using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS). Inclusion of Natal plum in the Marula nut bar increased the Cy-3-Sa, Cy-3-G content, antioxidants capacity and α-glucosidase inhibition compared to ingesting Marula nut separately at the internal phase. Adding Natal plum to the Marula nut bar increased bioaccessibility of Cy-3-Sa, Cy-3-G, quercetin, coumaric acid, syringic acid and ferulic acid to 80.2% and 71.9%, 98.7%, 95.2%, 51.9% and 89.3%, respectively, compared to ingesting the Natal plum fruit or nut separately.

scholarly journals Bioactive secondary metabolites from the leaves of Secamone africana (Olive.) Bullock

2020 ◽  
Vol 14 (5) ◽  
pp. 1820-1830
Author(s):  
Peter Sekandi ◽  
Jane Namukobe ◽  
Jane Namukobe ◽  
Robert Byamukama ◽  
Hoseah M. Akala ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Malaria Parasite ◽  
Therapeutic Potential ◽  
Coumaric Acid ◽  
Dihydroxybenzoic Acid ◽  
Meoh Extract ◽  
Isolation And Purification ◽  
Chromatographic Techniques ◽  
Bioactive Secondary Metabolites

Secamone africana leaves are used in the treatment of malaria and other ailments in Uganda. The aim of the study was to characterize the antiplasmodial compounds from the leaves of Secamone africana. The leaves were extracted sequentially using dichloromethane (DCM) and methanol (MeOH). The crude extracts and isolated compounds were evaluated for their antiplasmodial activity against the chloroquine sensitive Sierraleone I (D6) and chloroquine-resistant Indochina I (W2) strains of Plasmodium falciparum. Isolation and purification were done using chromatographic techniques including column chromatography and high performance liquid chromatography. The isolated compounds were characterized using spectroscopic methods. The MeOH extract (IC50 = 5.45 μg/mL) was found to be more active than the DCM extract (IC50= 15.93 μg/mL) against the D6 malaria parasite. Chemical investigation of the MeOH extract yielded one new compound; 2-(2,4-dimethyloxetan-2-yl) acetic acid (3) in addition to the six known compounds; α-linolenic acid (1), conduritol B (4), β-sitosterol (5), 3,4-dihydroxybenzoic acid (6), 4-hydroxybenzoic acid (7) and coumaric acid (8). The DCM extract yielded one known compound: 1-methyl cyclobutene (2). The presence of these compounds with good anti-plasmodial activities and other bioactivities reported in literature, appears to argue for the therapeutic potential of Secamone africana.Keywords: Secamone africana,, anti-plasmodial activity, chromatography, secondary metabolites.    

scholarly journals Evaluation of phenolic acids and phenylpropanoids in the crude drugs

Medicina ◽  
2007 ◽  
Vol 44 (1) ◽  
pp. 48 ◽  
Author(s):  
Liudas Ivanauskas ◽  
Valdas Jakštas ◽  
Jolita Radušienė ◽  
Audronis Lukošius ◽  
Algirdas Baranauskas
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Raw Materials ◽  
Therapeutic Agents ◽  
Reversed Phase ◽  
Coumaric Acid ◽  
Dihydroxybenzoic Acid ◽  
Simple Method ◽  
Analysis Techniques ◽  
Crude Drugs

Phenolic acids and phenylpropanoids have an important biological activity and are therapeutic agents of crude drugs. Development of validated analysis techniques of these phytotherapeutic agents (fingerprinting and assay procedures) is an important practice for efficacy, safety, and quality control of herbal drug preparations. The aim of the present work was to study analytical capabilities of the evaluation of selected phenolic acids and phenylpropanoids: caffeic acid, chlorogenic acid, cinnamic acid, coumaric acid, ferulic acid, gallic acid, protocatechuic (3,4-dihydroxybenzoic) acid, rosmarinic acid, vanillic acid, and vanillin. Optimization and validation procedures of rapid and simple method of reversed-phase high-performance liquid chromatography were carried out. The mobile phase of the optimized chromatographic method consisted of methanol and 0.5% acetic acid solvent in water. For the application of method, two kinds of raw materials were chosen: propolis and the Herba Origani. Coumaric acid is the dominating phenolic acid of propolis (2785 mg/g). Results of analysis of Herba Origani demonstrated high quantities (6376 mg/g) of rosmarinic and protocatechuic (1485 mg/g) acids in the samples.

scholarly journals Phytochemical, HPLC and FTIR Analysis of Methanolic Extract from Gracilaria dura (C Agardh) J Agardh.

2020 ◽  
Vol 10 (3) ◽  
pp. 114-118
Author(s):  
S S Sumayya ◽  
A S Lubaina ◽  
K Murugan
Keyword(s):  
Bioactive Compounds ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Sinapic Acid ◽  
Alkyl Halides ◽  
Biologically Active ◽  
Hydroxyl Groups ◽  
Coumaric Acid ◽  
Lifestyle Diseases ◽  
Gracilaria Dura

Marine algae are known to contain a wide variety of bioactive compounds. They are also rich in novel biomolecules and can be explored for the development of drugs to combat lifestyle diseases like cancer, diabetes etc. Red algae are known for their nutraceutical and functional importance. But there are a lot of limitations regarding their availability and in estimating which algal fractions are biologically active. Similarly, the mode of digestion of such compounds in human body is not yet properly traced. In this juncture, the present study was aimed to evaluate the phytochemical screening of the methanolic extract of the red alga, Gracilaria dura. Methanolic extract of G. dura showed the presence of reducing sugar, flavonoids, glycosides, tannins and terpenoids. Further, the HPLC analysis was attempted to fractionate the polyphenolics. Various phenolic acids such as of gallic acid, vanillic acid, sinapic acid, p-coumaric acid, hydroxybenzoic, phloroglucinol, catechol and cinnamic acid were identified. Subsequently, the methanolic solvent extract of G. dura was subjected to fourier transform infrared spectroscopy for the analysis of the functional groups. The results based on the spectral data of FTIR revealed the presence of aliphatic constituents containing alkanes, ketones, alkyl halides, hydroxyl groups etc. Thus, the observed finding envisages that methanolic extract of G. dura contained potential bioactive compounds which can be used for analysing the various biological activities. Keywords: Gracilaria dura, Phytochemical, Methanolic extract, HPLC, FTIR

scholarly journals Polyphenolic compounds in seeds from some grape cultivars grown in Serbia

2010 ◽  
Vol 75 (12) ◽  
pp. 1641-1652 ◽  
Author(s):  
Dejan Godjevac ◽  
Vele Tesevic ◽  
Milovan Velickovic ◽  
Ljubodrag Vujisic ◽  
Vlatka Vajs ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Polyphenolic Compounds ◽  
Hydroxycinnamic Acids ◽  
Hydroxybenzoic Acid ◽  
Qualitative And Quantitative ◽  
Esi Ms ◽  
Polyphenolic Composition ◽  
Grape Cultivars ◽  
Seed Extracts

Seed extracts from eight grape cultivars (Vitis vinifera) growing in Serbia were screened for their polyphenolic composition by means of HPLC/PDA/ESI/MS analysis. The study revealed 34 phenolic compounds belonging to the following groups: flavan-3-ol monomers, proanthocyanidins, flavonols, hydroxycinnamic acids and hydroxybenzoic acid derivatives. The quantities of the main constituents were determined using PDA/HPLC. Qualitative and quantitative differences among the cultivars were observed.

Characterisation of Polyphenolic Compounds in Viscum album L. and Allium sativum L. extracts

2017 ◽  
Vol 68 (7) ◽  
pp. 1677-1680 ◽  
Author(s):  
Svetlana Trifunschi ◽  
Melania F. Munteanu ◽  
Elena Pogurschi ◽  
Ramona Gligor
Keyword(s):  
Side Effects ◽  
Pharmaceutical Industry ◽  
Phenolic Acids ◽  
Allium Sativum ◽  
Sinapic Acid ◽  
Chemical Compounds ◽  
Viscum Album ◽  
Polyphenolic Compounds ◽  
Coumaric Acid ◽  
Gentisic Acid

There are numerous phytochemicals (isoflavones, flavonoids, catechins, anthocyanins, carotenoids, polyphenols, phenolic acids), which are largely used in the pharmaceutical industry. This research describes the process through which, one can obtain medicinal extracts from mistletoe (Viscum album L.) and garlic (Allium sativum L.) while giving an exact characterisation of the two extracts. The HPLC/MS analysis was employed in order to identify and evaluate the presence of phenolic acids (gentisic acid, chlorogenic acid, p-coumaric acid, ferulic acid and sinapic acid) and of flavonoids (hyperoside, isoquercitrin, rutin and luteolin). The results suggest that these medicinal extracts could replace the chemical compounds used in various therapies and which unfortunately have side effects on patients.

The influences of light-emitting diodes (LEDs) on the phenolic content and antioxidant enzymes of basil using a plant factory system

2021 ◽  
pp. 1-12
Author(s):  
Omar Almaghrabi ◽  
Yaaser Q. Almulaiky
Keyword(s):  
Antioxidant Capacity ◽  
High Performance ◽  
Light Emitting Diode ◽  
Syringic Acid ◽  
Coumaric Acid ◽  
Light Emitting ◽  
Phenolic Components ◽  
Plant Factory ◽  
Mda Content ◽  
Led Lights

We used two types of Ocimum basilicum plants and white light-emitting diode (LED) lights as controls (plant factory unit 1, PFU 1). We applied four composite LED light treatments, 125:125:125 white, red, blue (PFU 2), 0.0:250:125 white, red, blue (PFU 3), 125:125:57 white, red, blue (PFU 4) and 125:57:125 white, red, blue (PFU 5) to evaluate light quality effects on antioxidant capacity of O. basilicum plants. The results revealed that the composite lights of PFUs 2,3,4 and 5 were beneficial for the accumulation of flavonoids and glutathione but were not beneficial forthe proanthocyanidin content; the plants subjected to the PFUs 2,3,4 and 5 treatments had a higher laccase activity and proline content and a lower malondialdehyde (MDA) content, polyphenol oxidase activity and peroxidase activity than the control plants.Analysis via high-performance liquid chromatography revealed that protocatechuic acid, gentisic acid, chlorogenic acid, syringic acid, cinnamic acid, quercetin, apigenin, kaempferol, chrysin, p-hydroxybenzoic acid, p-coumaric acid, apigenin-7-glucoside, rutin, rosmarinic acid were the major phenolic components in the O. Basilicum extracts, and sufficient composite lighting of O. basilicum plants significantly enhanced these antioxidant concentrations. Our results indicate that the use of LEDs with different light qualities to irradiate O. basilicum significantly improved the antioxidant capacity which could be a beneficial for nutrition and health benefits.

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