scholarly journals Introduction of a complementary screening system for the detection of sexually transmitted infections in patients with sterile pyuria

2021 ◽  
Author(s):  
Helena Gil-Campesino ◽  
◽  
Laura Sante ◽  
Enrique Callejas Castro ◽  
María Lecuona
Keyword(s):  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Mycoplasma Genitalium ◽  
Epidemiological Data ◽  
Screening System ◽  
Effective Screening ◽  
Sexually Transmitted ◽  
Work Routine ◽  
Sterile Pyuria ◽  
A Prospective Study

Objective. The aim of the study was to evaluate a complementary screening system for the detection of sexually transmitted infections in patients with sterile pyuria. Material and methods. A prospective study was conducted using Real-time multiplex qPCR in 300 consecutive urine samples with data on sterile pyuria. STI prevalence and patient epidemiological data were analyzed. Results. A total of 29 positive cases (9.67%) were found: 16 Chlamydia trachomatis, 5 Mycoplasma genitalium, 5 Trichomonas vaginalis and 3 co-infections. The group with sexually transmitted infections was mainly composed of women (65.5%), of which one third was pregnant. Conclusion. This study presents an effective screening system for the detection of sexually transmitted infections that can be integrated into the laboratories’ work routine.

scholarly journals INCIDENCE OF SEXUALLY TRANSMITTED INFECTIONS: LOCAL STUDY IN UKRAINE

World Science ◽  
2018 ◽  
Vol 2 (8(36)) ◽  
pp. 4-7
Author(s):  
Fedorych P. V. ◽  
Mavrov G. I.
Keyword(s):  
Human Papillomavirus ◽  
Chlamydia Trachomatis ◽  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Local Study ◽  
Sexually Transmitted ◽  
Clinically Significant

Introduction.The structure of incidence of sexually transmitted infections is changing constantly. Information on such changes supports correct planning of clinical and diagnostic activities of institutions providing specialized medical care by qualified specialists.Objective:to investigate the prevalence of sexually transmitted infections with pathogens clinically significant to the genitourinary system in Ukraine and at the local level.Materials and methods. Polymerase chain reaction was used to test the biological material obtained from the genitourinary clinical specimens from subjects with sexually transmitted infections, who underwent clinical and laboratory examinations in Oleksandrivsk Clinical Hospital (Kyiv, Ukraine) for Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Trichomonas vaginalis, Human papillomavirus, and Neisseria gonorrhoea. During 2017, 607 subjects of both genders, including 295 (48.6%) females and 312 (51.4%) males, were examined. Their mean age was 32±3.5.Findings. Chlamydia trachomatis was found in 159 (26.2%) of 607 examined subjects – 85 males and 74 females. Mycoplasma hominis was found in 122 of 585 (21.1%) examined subjects – 64 males and 58 females. Mycoplasma genitalium, respectively, in 17 (6.62%) of 258 subjects – 6 males and 11 females. Ureaplasma urealyticum was found in the largest number of subjects (305, i.e. in 48.77% of 601 examined subjects) – 157 males and 148 females. Trichomonas vaginalis was found in 28 (5.23%) of 535 subjects – 15 males and 13 females. Human papillomavirus was found in 158 of 297 (53.2%) examined subjects – 88 males and 70 females. Neisseria gonorrhea was found in 33 of 297 (8.45%) subjects – 8 males and 25 females.Conclusions. As suggested by the local study of the sexually transmitted infections incidence in Ukraine, the most clinically significant for the genitourinary system are Human papillomavirus (53.2%), Ureaplasma urealyticum (48.77%), Chlamydia trachomatis (26.2%) and Mycoplasma hominis (21.1%). Therefore, tests for these pathogens in the specified region is currently the most appropriate during diagnostic examinations and counselling of subjects with genitourinary infections.

Sexually transmitted infections and localized scleroderma of the anogenital area

2020 ◽  
Vol 18 (3) ◽  
pp. 82-87
Author(s):  
E.S. Snarskaya ◽  
◽  
O.Yu. Olisova ◽  
Yu.A. Semenchak ◽  
◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Herpes Simplex ◽  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Mycoplasma Genitalium ◽  
Localized Scleroderma ◽  
Chronic Infections ◽  
Sexually Transmitted ◽  
Anogenital Area ◽  
Simplex Virus

Objective. To analyze the spectrum of sexually transmitted infections (Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma genitalium, and Neisseria gonorrhoeae) in patients with different clinical variants of localized scleroderma (LS) and scleroatrophic lesions in the anogenital area. Patients and methods. We examined 104 patients diagnosed with LS (including guttate morphea, linear scleroderma, scleroatrophic lichen, and atrophoderma of Pasini and Pierini) in V.A.Rakhmanov Clinic of Skin and Venereal Diseases. After clinical and morphological phenotyping, we selected 62 patients (17.7% of males and 82.3% of females) with scleroatrophic lesions in the anogenital area. All patients were tested for sexually transmitted infections, including Human papillomavirus (HPV) (high-risk types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68) and herpes simplex virus type 1 and 2 (HSV1/2) using polymerase chain reaction. Results. We found that 71% of patients with scleroatrophic lesions in the anogenital area had chronic infections, including those caused by Chlamydia trachomatis (29.5%), Trichomonas vaginalis (15.9%), Mycoplasma genitalium (13.6%), and Neisseria gonorrhoeae (2.3%). In 38.6% of cases, the development of scleroatrophic lesions was associated with bacterial vaginosis. One-third of patients (33.8%) were found to have high-risk HPV strains (type 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68) and HSV 1/2. Conclusion. In our opinion, anogenital location of LS can be a marker of chronic infections of the genitourinary system; such patients should be tested for sexually transmitted infections. Keywords: localized scleroderma; scleroatrophic lichen; anogenital location; vulvar lichen; penile lichen; sexually transmitted infections; bacterial vaginosis; herpes simplex virus; human papillomavirus

scholarly journals Association of Female Genital Schistosomiasis with the Cervicovaginal Microbiota and Sexually Transmitted Infections in Zambian Women

2021 ◽  
Author(s):  
Amy S Sturt ◽  
Emily L Webb ◽  
Lisa Himschoot ◽  
Comfort R Phiri ◽  
Joyce Mapani ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Mycoplasma Genitalium ◽  
Gardnerella Vaginalis ◽  
Sexually Transmitted ◽  
Weak Evidence ◽  
Key Species ◽  
Atopobium Vaginae ◽  
Female Genital

Abstract Background The cervicovaginal microbiota, including sexually transmitted infections (STI), have not been well-described in female genital schistosomiasis (FGS). Methods Women (aged 18-31, sexually active, non-pregnant) were invited to participate at the final follow-up of HPTN 071 (PopART) Population Cohort in January-August 2018. We measured key species of the cervicovaginal microbiota (Lactobacillus crispatus, L. iners, Gardnerella vaginalis, Atopobium vaginae and Candida) and STI (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis and Mycoplasma genitalium) using quantitative PCR (qPCR). We evaluated associations of microbiota and STI presence and concentration with FGS (qPCR-detected Schistosoma DNA in any of three genital specimens). Results The presence and concentration of key cervicovaginal species did not differ between participants with (n=30) or without FGS (n=158). A higher proportion of participants with FGS had T. vaginalis compared to FGS negative women (p=0.08), with further analysis showing that T. vaginalis was more prevalent among women with ≥2 Schistosoma qPCR positive genital specimens (50.0%, 8/16) than among FGS negative women (21.5% 34/158, p=0.01). Conclusions We found weak evidence of an association between T. vaginalis presence and FGS, with a stronger association in women with a higher burden FGS infection. Additional research is needed on potential between-parasite interactions, especially regarding HIV-1 vulnerability.

scholarly journals Are Swedish swingers a risk group for sexually transmitted infections?

2021 ◽  
pp. 095646242097310
Author(s):  
Nirina Andersson ◽  
Jennifer Ejnestrand ◽  
Yvonne Lidgren ◽  
Annika Allard ◽  
Jens Boman ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Risk Group ◽  
Mycoplasma Genitalium ◽  
Unprotected Sex ◽  
Sex Partners ◽  
Sexually Transmitted ◽  
High Risk Sexual Behavior ◽  
Vaginal Sex ◽  
The Mean

The aim of this study was to investigate whether Swedish swingers constitute a risk group for sexually transmitted infections (STIs). Two swinger clubs were invited to participate. At swinger meetings, members were offered an STI sampling kit and a questionnaire. Samples were analyzed for Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis using a multiplex real-time polymerase chain reaction assay. In total, 235 swingers participated (118 women and 117 men). Urogenital C. trachomatis prevalence was 1.7%. Urogenital M. genitalium prevalence was 7.6% for women and 4.3% for men. No one tested positive for N. gonorrhoeae or T. vaginalis. For women, the mean number of unprotected temporary sex partners within the last 12 months was four men (range 0–35) and three women (range 0–50). Among men, the mean number of unprotected temporary sex partners within the last 12 months was five women (range 0–50) and 0 men (range 0–10). During vaginal sex, 46.6% women and 38.5% men always used protection with a temporary sex partner. Swedish swingers did not seem to have an increased prevalence of STIs. However, there was high-risk sexual behavior with unprotected sex and multiple sex partners, thereby making them a vulnerable group for acquiring STIs.

scholarly journals Improved molecular laboratory productivity by consolidation of testing on the new random-access analyzer Alinity m

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Martin Obermeier ◽  
Monia Pacenti ◽  
Robert Ehret ◽  
Francesco Onelia ◽  
Rory Gunson ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Random Access ◽  
Mycoplasma Genitalium ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
B Virus ◽  
Immunodeficiency Virus ◽  
International Multicenter Study ◽  
Laboratory Productivity ◽  
Laboratory Workflow

AbstractObjectivesAutomated molecular analyzers have accelerated diagnosis, allowing earlier intervention and better patient follow-up. A recently developed completely automated molecular analyzer, Alinity™ m (Abbott), offers consolidated, continuous, and random-access testing that may improve molecular laboratory workflow.MethodsAn international, multicenter study compared laboratory workflow metrics across various routine analyzers and Alinity m utilizing assays for human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV), hepatitis B virus (HBV), high-risk human papillomavirus (HR HPV), and sexually transmitted infection (STI) (Chlamydia trachomatis [CT]/Neisseria gonorrhoeae [NG]/Trichomonas vaginalis [TV]/Mycoplasma genitalium [MG]). Three turnaround times (TATs) were assessed: total TAT (sample arrival to result), sample onboard TAT (sample loading and test starting to result), and processing TAT (sample aspiration to result).ResultsTotal TAT was reduced from days with routine analyzers to hours with Alinity m, independent of requested assays. Sample onboard TATs for standard workflow using routine analyzers ranged from 7 to 32.5 h compared to 2.75–6 h for Alinity m. The mean sample onboard TAT for STAT samples on Alinity m was 2.36 h (±0.19 h). Processing TATs for Alinity m were independent of the combination of assays, with 100% of results reported within 117 min.ConclusionsThe consolidated, continuous, random-access workflow of Alinity m reduces TATs across various assays and is expected to improve both laboratory operational efficiency and patient care.

High Prevalence of Sexually Transmitted Infections and Risk Factors Among HIV-Positive Individuals in Yunnan, China

2021 ◽  
Author(s):  
Wei Tu ◽  
Yu-Ye Li ◽  
Yi-Qun Kuang ◽  
Rong-Hui Xie ◽  
Xing-Qi Dong ◽  
...  
Keyword(s):  
Risk Factors ◽  
Sexually Transmitted Infections ◽  
Genital Tract ◽  
Mycoplasma Genitalium ◽  
Cross Sectional Study ◽  
Multiple Infections ◽  
Hiv Positive ◽  
Cross Sectional ◽  
Sexually Transmitted ◽  
High Prevalence

Abstract Background Yunnan has the highest rates of HIV in the country. Other treatable sexually transmitted infections (STIs) are associated with accelerated HIV transmission and poor ART outcomes, but are only diagnosed by syndromic algorithms. Methods We recruited 406 HIV-positive participants for a cross-sectional study (204 ART-naive and 202 ART). Blood samples and first-voided urine samples were collected. Real-time polymerase chain reaction methods were used for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Mycoplasma genitalium (MG). Syphilis and HSV-2 tests were also performed. Results Among 406 participants, the overall prevalence of STI was 47.0% and 45.1% in ART-naive individuals and 49.0% in ART individuals, respectively. Testing frequency was 11.6% (11.8% vs 11.4%), 33.2% (29.4% vs 37.1%), 3.2% (3.4% vs 3.0%), 2.0% (3.4% vs 0.5%) and 4.7% (6.4% vs 3.0%) for active syphilis, HSV-2, chlamydia, gonorrhoeae and genitalium. Percentage of multiple infections in both groups was 10.8% (22/204) in ART-naive participants and 9.9% (20/202) in ART participants. Females, age between 18 to 35 years, ever injected drugs, homosexual or bisexual, HIV/HBV coinfection, and not receiving ART were identified as risk factors. Self-reported asymptom was not eliminating of having a laboratory-diagnosed STI. Conclusions STI prevalence was 47.0% (45.1% vs 49.0%), HSV-2, syphilis and MG were the most common STIs in HIV-infected individuals. We found high prevalence (6.4%) of Mycoplasma genitalium in ART-naive individuals. ART can reduce the diversity of STI-HIV coinfection but not the prevalence. HIV-positive individuals tend to neglect or maybe hide their genital tract discomfort, thus we suggest strengthening STI joint screening and treatment services among HIV-infected individuals whether they describe genital tract discomfort or not.

scholarly journals Male Urethritis Caused by Trichomonas vaginalis

2021 ◽  
Vol 79 (1) ◽  
pp. 71-73
Author(s):  
Ângela Roda ◽  
João Borges-Costa
Keyword(s):  
Sexually Transmitted Infections ◽  
Sexually Transmitted Diseases ◽  
Trichomonas Vaginalis ◽  
Unusual Case ◽  
Parasitic Infection ◽  
Men And Women ◽  
Sexually Transmitted ◽  
Molecular Assays ◽  
The Past ◽  
The Impact

Trichomoniasis is one of the most common sexually transmitted infections worldwide. In women, Trichomonas vaginalis infection may present with vaginitis, cervicitis, or pelvic inflammatory disease, while in men it is mainly asymptomatic or causes mild and transient symptoms of urethritis, epididymitis, or prostatitis. In the past, little importance had been given to the impact of T. vaginalis infection on men’s health, since it was believed to be a self-limited condition without sequelae. However, there is growing evidence it is associated with more serious disorders in both men and women and efforts to diagnose and treat this parasitic infection have increased. Recent advances in testing for sexually transmitted diseases using multiplex molecular assays have increased diagnostic opportunities for T. vaginalis infection, especially in men, as detection of the parasite by traditional methods is much more challenging. We describe an unusual case of male urethritis caused by T. vaginalis observed in our consultation of Sexually Transmitted Infections.

scholarly journals Prevalence of sexually transmitted infections among pregnant women attending antenatal clinics in West Coast Region of The Gambia

2021 ◽  
Vol 21 (2) ◽  
pp. 585-592
Author(s):  
Alphonsus Isara ◽  
Aru-Kumba Baldeh
Keyword(s):  
Pregnant Women ◽  
Sexually Transmitted Infections ◽  
West Coast ◽  
Trichomonas Vaginalis ◽  
The Gambia ◽  
Age Group ◽  
The West ◽  
Sexually Transmitted ◽  
Neisseria Gonorrhoea ◽  
Coast Region

Background: Sexually Transmitted Infections (STI) are the second most common cause of healthy life years lost by women in the 15 – 44 years age group in Africa. Aim/Objective: To determine the prevalence of STIs among pregnant women attending antenatal care (ANC) clinics in the West Coast Region of The Gambia. Materials and Methods: Blood, urine, and high vaginal swabs samples from 280 pregnant women attending ANC in Brika- ma District Hospital, Brikama, and Bandung Maternity and Child Health Hospital, Bandung were examined. Serum samples were tested for HIV using western blot technique and for syphilis using the Venereal Disease Research Laboratory (VDRL) test, and rapid plasma regimen. Candida albicans, Group B Streptococcus and Neisseria gonorrhoea were identified using Analytical Profile Index (API). Direct urine microscopy was used to identify C. albicans and Trichomonas vaginalis while Chlamydia trachomatis was identified using Direct Fluorescent Antibody (DFA) test. Results: The overall prevalence of STIs was 53.6%. The pathogenic agents isolated were Candida albicans (31.8%), Strep- tococcus agalactiae (15.0%), Treponema pallidum (6.8%), HIV (5.7%), Trichomonas vaginalis (3.9%), Neisseria gonorrhoea (1.8%) and Chlamydia trachomatis (0.7%). STIs were more prevalent among women in the younger age group of 15 – 24 years (54.7%), unemployed (54.0%), Primipara (62.3%), and in the third trimester of pregnancy (72.7%). Conclusion: A high prevalence of STIs was found among pregnant women attending ANC in the West Coast region of The Gambia. Public health intervention programmes should be strengthened to promote the sexual and reproductive health of pregnant women in The Gambia. Keywords: Sexually transmitted infections; pregnant women; antenatal clinics; The Gambia.

Sign in / Sign up

Export Citation Format

Share Document