scholarly journals Antioxidant Activity Sonneratia caseolaris Leaves Extract at Different Maturity Stages

2020 ◽  
Vol 8 (3) ◽  
pp. 130
Author(s):  
Winarti Winarti ◽  
Boedi Setya Rahardja ◽  
Sudarno Sudarno
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Heart Attack ◽  
Total Content ◽  
Reactive Component ◽  
Mature Leaves ◽  
Maturity Stages ◽  
Sonneratia Caseolaris ◽  
Young Leaves ◽  
Ic50 Values

Free radical is a reactive component because it has one or more electrons. Free radical is very dangerous for our body because it can cause degenerative diseases, such as cancer, heart attack, and aging. Cancer and heart attack are one of the most killer disease. All we can do to decrease the free radical effect is consume antioxidant. S. caseolaris leaves has antioxidant activity, but it has known yet which stage of maturity is the best one for produce the highest antioxidant activity. This purpose of this research is knowing the influence between maturity and antioxidant activity at different maturity stages. This method of this research is experimental with Completely Randomized Design. The treatment in this research is vitamin C, fresh leaves in ethanol 96% extract, young leaves in ethanol 96% extract, and mature leaves in ethanol 96% extract. The main parameter of this research is IC50 values and the secondary parameters are total content of phytochemical in every extract. Data analysis using descriptive method with 4 treatments and five replications. The result of this research show that the maturity has influence in antioxidant activity. Inhibitory concentration 50 (IC50) values of shoot leaves extract, young leaves extract and mature leaves extract is 12.0013 ppm, 13.9915 ppm and 14.6613 ppm. All of them are called antioxidant which has very strong activities. The highest phytochemical compound is found on shoot leaves extract with ethanol 96% solvent.

scholarly journals Antioxidant Activity Test of Effervescent Granules Morinda citrifolia L Leaf Extract with DPPH Free Radical Absorption Method

2021 ◽  
Vol 5 (4) ◽  
pp. 365-368
Author(s):  
Muthmainah Tuldjanah ◽  
Niluh Puspita Dewi ◽  
Dian Rahmawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Natural Antioxidants ◽  
Morinda Citrifolia ◽  
Absorption Method ◽  
Immersion Method ◽  
Activity Test ◽  
Ic50 Values

Morinda citrifolia. L leaf is a plant that has the potential as an antioxidant due to the presence of compounds in the form of flavonoids and other phenolic compounds that can function as natural antioxidants. In connection with this, research was carried out to Morinda citrifolia. L formulate leaf extract in the form of effervescent granules. Morinda citrifolia leaf extract was macerated with ethanol 96% as solvent. Phytochemical screening showsthat the extract contains flavonoids, tannins, saponins and alkaloids. The extract was formulated with various concentrations of F1 (20%), F2 (25%), F3 (4%). Determination of antioxidant activity using the DPPH free radical immersion method. The results showed that the concentration in the granules influenced antioxidant activity with IC50 values of 109.05 ppm, 101.33 ppm and 73.28 ppm, respectively

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

Assesment Of Antioxidant Activity Test Of KERSEN Leaf (Muntingia calabura L.) And Epiphyte with DPPH (2.2-Diphenyl-1-Picrylhidrazyl)

2020 ◽  
Vol 1 (2) ◽  
pp. 60-66
Author(s):  
Nurlutfiyyah Aini ◽  
Fatmawati ◽  
Nita Parisa
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Linear Regression Analysis ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
Human Needs ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Free Radical Activity ◽  
Muntingia Calabura

Antioxidant is very important to give protection against free radical activity and highlyreactive molecules that could lead in slowing the progression of degenerative disease.In case of degenerative disease, internal antioxidant cannot neutralize the increasingconcentration of free radical. Because of that, human needs external antioxidant.Kersen (Muntingia calabura L.) is a plant that is known for its antioxidant content.Plants containing antioxidant experience is kersen (Muntingia calabura L.). Researchstudy to determine the antioxidant activity of Kersen plant and knows the differenceof antioxidant activity, based on the process of extract and infusion. Research wasdone by experimental study which was oriented in testing antioxidant activity in(Morinda citrifolia L.) extract and infusion. Extraction was done by using 96% ethanolas solvent, meanwhile infusion was made by using aquadest. Extract and infusionwas divided into group of concentration and antioxidant activity was tested byDPPH(2,2-Diphenyl-1-Picrylhidrazyl) method by measuring the absorbance usingspectrophotometer at 520 nm wavelength. Percentage of DPPH inhibition and IC50then analyzed using linear regression analysis. Ethanolic extract of kersen leaf andepiphyte had IC50 value of 113,801 ppm and 98,7802 ppm, respectively. Kersen leafinfusion showed 191,7624 ppm IC50 values, besides its epiphyte had 131,6750 ppm.Antioxidant activity of Muntingia calabura L. in the order from kersen leaf anepiphyte, and epiphyte extract has a higher antioxidant content than others.

Antioxidant Activity of Some Selected East Borneo Plants

2015 ◽  
Vol 4 (1) ◽  
pp. 58
Author(s):  
Islamudin Ahmad ◽  
Risky Sulistiarini ◽  
Laode Rijai
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Native Plants ◽  
Ethyl Acetate Extract ◽  
Model Systems ◽  
Brucea Javanica ◽  
Butanol Extract ◽  
Sonneratia Caseolaris ◽  
Free Radical Activity

The native plants of East Borneo the <em>Costus specious </em>(Koening) J.E. Smith stem, <em>Lagerstroema spesiosa</em> Pers leaf, <em>Cerbera mangans </em>L leaf, <em>Vitis trifolia </em>L fruit., <em>Scurrula atropurpurea </em>(Blume) Danser root, <em>Brucea javanica </em>(L.) Merr. leaf, <em>Lygodium microphyllum, Bidens Chinensis </em>Willd., <em>Sonneratia caseolaris </em>L. peel, <em>Sonneratia caseolaris </em>L. stem is almost under-explored for their potensial benefits. They were extracted by the solvents of increasing polarity (n-hexane, ethyl acetate, and n-butanol) were tested for their free radical activity against DPPH (2,2-diphenyl-1-picrylhydrazyl). The ethyl acetate extract of <em>Costus spesiosus </em>(Koening) J. E. Smith antioxidant were screnned concentration of 68 ppm, similarly athyl acetate extract of <em>Vitis trivolia</em> L., showed antioxidant activity at 64,30 ppm. As the ethyl acetate extract of <em>Scurrulla atropurpurea </em>(Blume), <em>Brucea javanica</em> (L.) Merr, <em>Lygodium microphyllum</em> and <em>Sonneratia caseolaris</em> L. stem, showed antioxidant activity at 273,52 ppm, 91,12 ppm, 17,39 ppm and 7,03 ppm. N-butanol extract of <em>Lagerstroema spesiosa </em>Pers,<em> Cerbera mangans</em> L, <em>Bidens chinensis</em> Willd, and <em>Sonneratia caseolaris</em> L. peel showed 8,37 ppm, 128,59 ppm, 18,17 ppm and 54,29 ppm antioxidant activity using DPPH model systems. Owing to the property, the studies can be further extended to exploit them for their possible application for preservation of food products as well as their use as health supplements.

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BUAH BUNI (Antidesma bunius (L.) SPRENG)

2016 ◽  
Vol 3 (2) ◽  
pp. 159-163
Author(s):  
Arif Rahman ◽  
Abd Malik ◽  
Aktsar Roskiana Ahmad
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Free Radical Scavengers ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dried Fruit ◽  
Ethanol Extracts

Free radicals play a role in the occurrence of various degenerative diseases that require free-radical scavengers or antioxidants. Buni fruit (Antidesma bunius (L.) Spreng) has the bioactive components are Anthocyanins (flavonoids) that serves to the free radicals. This study aimed to measure the antioxidant activity of theethanol extract contained 70% fruit Buni obtained by using the method of nitric oxide. Simplicia buni macerated dried fruit with 70% ethanol. Extracts were obtained in the test antioxidant activity against nitric oxide radicals. The antioxidant activity against free radical absorbance measured by means of UV-Vis spectrophotometry at a wavelength of 546 nm and calcul ated IC50 values. The results shows that the fruit buni has potential as a free radical with IC50 value of 2.28 µg/mL and a comparison of quercetin with IC50 value of 5.88 µg/mL.

scholarly journals Antioxidant activity and phenol content of extracts of bark, stems, and young and mature leaves from Blepharocalyx salicifolius (Kunth) O. Berg

2016 ◽  
Vol 76 (4) ◽  
pp. 898-904 ◽  
Author(s):  
E. Habermann ◽  
M. Imatomi ◽  
F. C. Pontes ◽  
S. C. J. Gualtieri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Plant Secondary Metabolites ◽  
Total Phenolic ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Phenol Content ◽  
Mature Leaves ◽  
Dpph Method ◽  
Young Leaves

Abstract Phenolic compounds are a group of plant secondary metabolites known to have a variety of bioactivities, including the ability to function as antioxidants. Because of the side effects of the use of synthetic substances, the search for natural and less toxic compounds has increased significantly. This study was designed to evaluate the antioxidant activity and phenol content of hexane, ethyl acetate, and aqueous extracts of the bark (suber) and stems as well as the young and mature leaves of Blepharocalyx salicifolius. The extracts were obtained by extraction with organic solvents and subsequent fractionation by chromatographic partition coefficient. Preliminary tests for the presence of antioxidants were performed using bioautography in thin-layer chromatography. The antioxidant activity of the extracts was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and the phenol content of the extracts was quantified using the Folin-Ciocalteu technique. The results showed that 9 of the 12 extracts evaluated displayed very strong antioxidant activity and three displayed moderate activity. Aqueous extracts of the young leaves and bark and the ethyl acetate extract of the young leaves showed the highest levels of antioxidant activity and total phenolic content (TPC). A correlation was observed between TPC and antioxidant activity index (AAI) with a correlation coefficient (r2) of 0.7999. Thus, the high phenol content of B. salicifolius extracts and its correlation with antioxidant activity provide substrates for further studies.

scholarly journals EVALUATION OF THE ANTIOXIDANT ACTIVITY OF CAROTENOID EXTRACT FROM BACTERIAL STRAINS ISOLATED IN NUI CAM, TINH BIEN DISTRICT, AN GIANG PROVINCE

2020 ◽  
Vol 1 (40) ◽  
pp. 50-57
Author(s):  
Lam Hong Bang ◽  
Nha Thanh Vo ◽  
Luong Vien Van
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Control Sample ◽  
Bacterial Strains ◽  
Radical Reduction ◽  
Ic50 Values

The research was conducted to evaluate the antioxidant activity of carotenoid extract from bacteria strainsisolated in An Giang Province. There by, the strain of bacteria with the best antioxidant capacity was selected. The results of DPPH free radical reduction survey on crude carotenoid extracts of the tested bacterial strains showed that all the crude carotenoid extracts of these strains have good DPPH free radical reductionability. Carotenoid extracts from strains NC4-3, NC8-3, NC1-6, NC3-3 and NC7-4 have IC50 values of 2,88; 3,30; 3,45; 5,15 và 9,05 mg/mL, respectively. This showed that the carotenoid extracts of these clones have lower antioxidant activity than the b-carotene control (IC50 = 2,70 mg/mL). Remarkably, the carotenoid extract of theNC4-3 strain had the best antioxidant activity of the 5 strains examined (IC50 = 2,88 mg/mL), only lower than the antioxidant activity of the control sample bcarotene.

scholarly journals Comparison of Antioxidant Activity of Ethanolic, Methanolic, n-Hexan, and Aqueous Extract of Parkia speciosa Peel based on Half -Maximal Inhibitory Concentration Through Free Radical Inhibition

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Fafa Nurdyansyah ◽  
Dyah Ayu Widyastuti
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Water Extract ◽  
Bark Extract ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Rotary Vacuum ◽  
Potential Content

The objectives of this study was to determine the half maximum inhibitory concentration (IC50) from four types of Parkia speciosa peel extracts (ethanol, methanol, n-hexane, and aqueous)  through DPPH free radical inhibition. First Parkia’s peel extract made by drying the Parkia’s peel that has been sorted, then crushed and mashed with a blender. Parkia’s powder then macerated for 3 replication using each type of solvent and then solvent evaporation was carried out using a rotary vacuum evaporator. The evaporated extract produced then tested for antioxidant activity using the IC50 method and phytochemical screening was performed to analyze the potential content of functional compounds. The results showed that all types of solvents dissolve alkaloid compounds (except water extract), flavonoids, saponins, tannins, and phenols. IC50 values produced from the four types of petai bark extract using methanol, ethanol, water, and n-hexane solvents sequentially were 76.92; 111; 136; and 201 ppm. Methanol extract had the lowest IC50 value of 76.92 ppm which resulted that the methanol extract of petai skin had a strong (active) antioxidant strength compared to others.Keywords: Antioxidant, Exctract, Parkia’s peel, IC50

scholarly journals Optimization of Flavonoids Extraction Process in Panax notoginseng Stem Leaf and a Study of Antioxidant Activity and Its Effects on Mouse Melanoma B16 Cells

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2219 ◽  
Author(s):  
Chun-Yan Dai ◽  
Peng-Fei Liu ◽  
Pei-Ran Liao ◽  
Yuan Qu ◽  
Cheng-Xiao Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Sodium Dodecyl ◽  
Radical Scavenging ◽  
Panax Notoginseng ◽  
Extraction Process ◽  
Utilization Rate ◽  
Leaf Extracts ◽  
Ic50 Values ◽  
Stem Leaf

The Panax notoginseng (P. notoginseng) stem leaf is rich in flavonoids. However, because of a lack of research on the flavonoid extraction process and functional development of P. notoginseng stem leaf, these parts are discarded as agricultural wastes. Therefore, in this study, we intend to optimize the extraction process and develop the skin-whitening functions of P. notoginseng stem leaf extracts. The extraction process of the stem and leaf of P. notoginseng flavonoid (SLPF) is optimized based on the Box–Behnken design (BBD) and the response surface methodology (RSM). The optimum extraction conditions of the SLPF are as follows: the extraction time, the ethanol concentration, the sodium dodecyl sulfate (SDS) content and the liquid material ratio (v/w, which are 52 min, 48.7%, 1.9%, and 20:1, respectively. Under the optimal extraction conditions, the average total SLPF content is 2.10%. The antioxidant activity and anti-deposition of melanin of mouse B16 cells of P. notoginseng stem leaf extracts are studied. The results indicate that the EC50 values of reducing activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities, the superoxide anion removal ability, and the 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) free radical removal ability are 7.212, 2.893, 2.949, and 0.855 mg/mL, respectively. The extracts IC50 values of the tyrosinase and melanin synthesis are 0.045 and 0.046 mg/mL, respectively. Therefore, the optimal processing technology for the SLPF obtained in this study not only increases its utilization rate, but also decreases material costs. The extracts from the P. notoginseng stem leaf may be developed as food or beauty products.

scholarly journals Antioxidant Activity and Total Flavonoid of Carica papaya L. Leaves with Different Varieties, Maturity and Solvent

2019 ◽  
Vol 39 (1) ◽  
pp. 54 ◽  
Author(s):  
Fatma Zuhrotun Nisa ◽  
Mary Astuti ◽  
Sofia Mubarika Haryana ◽  
Agnes Murdiati
Keyword(s):  
Antioxidant Activity ◽  
Carica Papaya ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Water Extraction ◽  
Antioxidant Power ◽  
Mature Leaves ◽  
Dpph Method ◽  
Young Leaves ◽  
Ferric Reducing Antioxidant Power

Carica papaya leaves are one of the vegetables consumed by Indonesian people, especially in Java Island. Carica papaya is easy to grow in Indonesia and has many variants, so, Carica Papaya leaves is a local potent to be developed for functional food and nutraceutical. The aim of this study was to investigate antioxidant activity and total flavonoids of Carica papaya leaves with different varieties, maturity and solvent. Carica papaya leaves (CPL) was firstly extracted by methanol to select two CPLs with high antioxidant capacity and total flavonoid. The two selected CPLs were further tested with different ages mainly young and mature leaves. One selected CPL was further tested with different extraction solvents. Antioxidant activity was determined by 2.2 diphenyl-1-picrylhydrazyl, DPPH and Ferric reducing antioxidant power, FRAP. This study used five varieties of Carica papaya leaves, namely Bangkok, California, Purple, Golden and Grendel. The result showed that Golden and Grendel varieties had a higher percentage of radical scavenging property than the others, which was 78.37% and 77.40% by the DPPH method. Grendel and Purple had a higher percentage of radical scavenging property, which was 45.82 and 34.32 mmol/mg. Grendel and Purple had a higher total flavonoid property, which was 50.33 and 46.02 µg/g. Mature leaves had a higher percentage of radical scavenging property than young leaves by DPPH and FRAP methods. Mature leaves had a higher total flavonoid property than young leaves in both Grendel and Purple. Grendel had a higher antioxidant activity and a higher total flavonoid property than Purple. Grendel with water extraction had a higher antioxidant activity by DPPH and FRAP methods. The total flavonoid of Grendel papaya leaves’ extract with water extraction was lower than ethanol 70% and methanol.

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