Haemostatic Shifts in Rats at Combined Hyper- and Hypothermic Exposure and Their Correction with Vitamins A, E, B6, B9, B12, P

2020 ◽  
pp. 394-400
Author(s):  
Anton V. Samoylov ◽  
◽  
Tat’yana Yu. Astakhova ◽  
Vladimir G. Solov’ev ◽  
◽  
...  
Keyword(s):  
Coagulation Factors ◽  
The Body ◽  
Laboratory Animals ◽  
Control Group ◽  
Thrombin Time ◽  
White Rats ◽  
Test Parameters ◽  
Significant Acceleration ◽  
Coagulation Testing ◽  
Therapeutic Doses

This study aimed to assess the state of haemostasis at supplementation of the diet with vitamins and the possibility of correcting changes in coagulation under combined thermal effects on the body of laboratory animals (white rats). The animals were kept on a nutritious diet with sufficient intake of proteins, fats, carbohydrates and trace elements. As part of daily servings in addition to the diet, the experimental group received vitamins A, E, B6, B9, B12 and P in therapeutic doses adequate to those recommended for humans; the control group did not receive vitamins. After 14 days, part of the animals from both groups were subjected to combined thermal stress. At all painful manipulations the animals were anesthetized with ethoxyethane. Blood samples were taken in a syringe from the jugular vein exposed by an ovalshaped incision immediately after stress exposure. For coagulation testing, blood was stabilized with a 3.8 % sodium citrate solution in a ratio of 1 to 9. Subsequent blood treatment met the requirements accepted for coagulation testing. The following haemostatic parameters were studied: platelet count, activated partial thromboplastin time, prothrombin time, thrombin time, antithrombin III activity, as well as content of fibrinogen and soluble fibrin monomer complexes. The obtained results showed that the combined thermal effect causes a significant acceleration of continuous blood coagulation (consumption thrombocytopenia, hypofibrinogenaemia and mismatch of clotting test parameters) in rats. Additional vitaminization restrains the severity of these changes, which is manifested in the absence of platelet consumption, a decrease in the consumption of plasma coagulation factors and the preservation of a high antithrombin potential.

scholarly journals The effect of the developed composition of lactic acid bacteria isolated from Carpathian cheese, on the body of laboratory animals

2021 ◽  
Vol 23 (94) ◽  
pp. 9-15
Author(s):  
I. I. Kushnir ◽  
O. Y. Tsisaryk
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Average Concentration ◽  
The Body ◽  
Laboratory Animals ◽  
Control Group ◽  
Morphological Parameters ◽  
White Rats ◽  
Probiotic Strains ◽  
Positive Effect

Data on the study of the effect of a consortium of lactic acid bacteria on the morphological parameters of blood and the state of the intestinal microflora of white rats using a composition of probiotic strains of L. lactis, Lb. plantarum and E. durans SB18 in a ratio of 50:40:10. Thus, when determining the morphological parameters of the blood of rats at 14 days of use found a positive effect of probiotic strains of lactic acid bacteria on the hematopoietic function of laboratory animals. In particular, a significant increase in hemoglobin concentration, erythrocyte count, leukocyte count and hematocrit value was found by 14.9, 14.0, 22.5 % (P < 0.05) and 5.7 %, respectively, compared with the control group. In addition, the use of the drug for 14 days caused an increase in the average concentration of hemoglobin in the erythrocyte (MCНS) by 9.8 % (P < 0.05) and a tendency to decrease the average hemoglobin in the erythrocyte (MCН) and the average erythrocyte volume (MCV) by 0.45 and 9.0 %, respectively, compared with the control group. Both central and peripheral organs of the immune system were also significantly affected. In particular, the relative weights of the thymus and spleen increased by 21.1 (P < 0.05 ) and 9.8 % (P < 0.05), respectively. When determining the leukogram of peripheral blood of rats, it was found that the use of the study drug in animals of the experimental group caused a tendency to increase the number of neutrophils, lymphocytes and monocytes by 6.0, 0.5 and 19.8 %, respectively, with a slight decrease in eosinophils. The use of a composition of a consortium of lactic acid bacteria stimulated an increase in lacto- and bifidobacteria content of the large intestine of white rats. In particular, a significant increase in the number of bifidobacteria and lactobacilli was found by 4.3 and 5.2 % (P < 0.001), respectively, relative to the animals of the control group. In addition, 14-day application of the studied composition of probiotic strains contributed to a probable reduction in the number of opportunistic and putrefactive microflora. The number of Escherichia coli, Staphylococcus aureus and fungi of the genus Candida significantly decreased relative to control by 4.7 (P < 0.01), 2.6 (P < 0.05) and 13.3 % (P < 0.001), respectively, all this indicated the recovery of the body of laboratory animals. The positive effect of lactic acid bacteria of the formed composition persists until the 21st day of the experiment.

scholarly journals The degree of cumulation of the “Bendamin” drug in the body of white rats

2019 ◽  
Vol 21 (94) ◽  
pp. 82-85
Author(s):  
I. S. Varkholiak ◽  
B. V. Gutyj
Keyword(s):  
Total Bilirubin ◽  
White Blood Cells ◽  
Weight Ratio ◽  
The Body ◽  
Laboratory Animals ◽  
Control Group ◽  
Internal Organs ◽  
White Rats ◽  
Chronic Poisoning ◽  
Experimental Group

Repeated introduction into the body of an animal of a medicinal substance can lead to its accumulation in the tissues of the body. Therefore, knowledge of the cumulative properties of substances or conditions that may lead to cumulations, are especially important for understanding the pathogenesis of intoxication, because this phenomenon often lies at the basis of acute and chronic poisoning. The purpose of the work was to study the degree of cumulation of the “Bendamin” preparation in the body of white rats. The properties of the cumulative drug were studied in 12 white rats weighing 150–160 g. Rats were divided into 2 groups: control and experimental. To determine the cumulative properties of Bendamin, it was administered to laboratory animals starting at a dose of 0.1 DL50, with a sequential increase in the dose of 1.5 times every 4 days. It was found that the cumulative coefficient of Bendamin was 8.31 units, indicating that the drug does not exhibit cumulative effects. In determining the mass coefficients of the internal organs in the animals of the experimental group, the tendency to increase the weight and liver mass coefficients was determined, respectively, by 5.2 and 5.1%. The weight ratio of the heart of the animals of the experimental group increased by 5.7% relative to the control group. After studying the morphological parameters of blood of rats at 24 days of the experiment for studying the cumulative properties of the “Bendamin” drug, an increase in the number of erythrocytes to 6.13 ± 0.27 T/L, white blood cells to 8.42 ± 0.54 G/L and hemoglobin up to 132.4 ± 2.44 g/l. In the analysis of leukocyte profile in rats, there was a decrease in the number of eosinophils and monocytes, respectively, at 1.1 and 1.3% relative to the control group. The number of lymphocytes in the blood of the experimental group of rats increased by 1.5%, while neutrophils by 0.9% compared to the control. When studying the level of total protein, its small increase was determined by 5.2%. The activity of aminotransferases in the blood serum of experimental rats on the 24th day of the experiment for the study of cumulative properties of the drug also increased by 13 and 8% relative to the control group. Also, creatinine and total bilirubin levels in their rat blood were decreased by 3.1 and 8.8%, respectively.

TOXICOLOGICAL PROFILE OF “BIOSOLBI” DRUG

1970 ◽  
pp. 67-69
Author(s):  
E. K. Rakhmatullin ◽  
O. D. Sklyarov
Keyword(s):  
Drug Toxicity ◽  
Probit Analysis ◽  
Laboratory Animals ◽  
Control Group ◽  
Hazard Class ◽  
Water Ingestion ◽  
White Rats ◽  
Poorly Soluble ◽  
And Behavior ◽  
Purebred Dogs

The article presents the results of a study of the "Bisolbi" drug toxicity (powder of light ash color, poorly soluble in water). When it is mixed with water it forms a suspension of particles that settle rapidly. Values of acute drug toxicity were determined on rats. We studied groups of six animals of the same sex, as well as similar control ones. The "Bisolbi" drug was injected to white rats intragastrically, males weighing 310 ... 320 g in doses of 2500 and 2740 mg / kg. Each dose was used in six animals; distilled water (3 ml) was used for the controls. The LD50 was calculated by the probit analysis method proposed by Litchfield and Wilcoxon modified by Z. Roth. When administered orally, an atraumatic metal probe was immersed in the stomach. Within 14 days monitored the overall health status and behavior of animals, the manifestation or absence of symptoms of intoxication; noted the features of feed and water ingestion, assessed the condition of the coat, physiological functions. Then groups of experimental rats were euthanized and pathomorphologically examined. We studied the effect of "Bisolbi" with repeated introduction and on not purebred dogs. Two groups of 3-4 years of age were completed with an average initial body weight of 13.63 ... 15.11 kg. Before use, the additive was thoroughly mixed with feed. The drug was injected during 31 days at a dose of 0.5 g / kg. Dogs of the control group (three) were fed wheat flour. After 15 and 31 days in laboratory animals in order to characterize the general condition in the blood, the amount of protein, urea, glucose, creatinine, cholesterol were determined. Based on studies it was found that the drug daily application by animals, is low toxic and safe, does not provoke the development of pathological reactions. According to the Hodge and Sterner classification "Bisolbi" can be attributed to the 6th class of toxicity - relatively harmless. Accordingto GOST 12.1.007-76 LD50 of the drug is more than 151 mg / kg, but less than 5000 mg / kg it is the 3rd hazard class (moderately hazardous).

scholarly journals Preclinical evaluation of the veterinary drug “Amoxidev 15” for its use in surgical and obstetric practice

2021 ◽  
Vol 23 (103) ◽  
pp. 109-115
Author(s):  
L.-M. Kostyshyn ◽  
R. Sachuk ◽  
Ye. Kostyshyn ◽  
O. Katsaraba
Keyword(s):  
Body Weight ◽  
Soft Tissues ◽  
Control Level ◽  
Subcutaneous Administration ◽  
The Body ◽  
Laboratory Animals ◽  
Veterinary Drug ◽  
Intragastric Administration ◽  
Toxicological Evaluation ◽  
White Rats

Suspension for injection “Amoxidev 15” is prescribed to fur-bearing animals (mink, fox), dogs and cats for the treatment of respiratory diseases (tonsillitis, tracheitis, pneumonia, bronchitis, rhinitis, sinusitis, bronchopneumonia), digestive (gastritis, enteritis, enteritis). genitourinary systems (nephritis, urethritis, urocystitis, mastitis, metritis, agalactia), musculoskeletal system (arthritis, osteoarthritis, joint injuries, tendonitis, hoof lesions), skin and soft tissues (eczema, dermatitis) caused by sensitive drug by microorganisms, including colibacillosis, streptococcus, bronchopneumonia, etc. Toxicological evaluation of the veterinary drug “Amoxidev 15” under the conditions of acute and subacute toxicological experiments on a model of white rats. According to the results of an acute toxicological experiment with intragastric administration of the drug “Amoxidev 15” white rats DL50 could not be calculated because the death of laboratory animals was not detected within 14 days after administration. The maximum administered dose (in absolute weight of the drug) was 20000.0 mg/kg body weight, which allows to refer the drug to class VI toxicity of relatively harmless substances (DL50 > 15000 mg/kg body weight), and the degree of safety to class IV – low-hazard substances (DL50 > 5000 mg/kg). According to the results of an acute toxicological experiment with subcutaneous administration of the drug “Amoxidev 15” white rats DL50 could not be calculated because the death of laboratory animals was not detected within 14 days after administration, the maximum dose was 5000.0 mg/kg body weight, therefore, the drug “Amoxidev 15” when administered subcutaneously by toxicity can be classified as class VI substances relatively harmless (DL50 Subcut > 4500.0 mg/kg). When administered subcutaneously to white rats, the drug “Amoxidev 15” under conditions of subacute toxicological experiment in doses of 0.1–1.0 ml/kg does not cause hemo-, hepato- and nephrotoxic effects on the body of laboratory animals, although 3-day administration of the drug in a dose 1.0 ml/kg body weight caused an increase in the activity of hepatospecific enzymes ALT and AST by 12.5 and 11.1 % (P < 0.05), respectively, relative to the control, which was restored to the control level 7 days after cessation.

COMPARATIVE ASSESSMENT OF THE FUNCTIONAL PROPERTIES OF THE FOOD ADDITIVE «TOMATNAYA» OBTAINED BY VARIOUS TECHNOLOGIES

2021 ◽  
Author(s):  
Е.П. ВИКТОРОВА ◽  
Е.В. ЛИСОВАЯ ◽  
М.В. ЛУКЬЯНЕНКО ◽  
Н.Н. КОРНЕН ◽  
Е.В. КУЗЬМИНОВА ◽  
...  
Keyword(s):  
Functional Properties ◽  
Antioxidant Properties ◽  
Food Additive ◽  
The Body ◽  
Innovative Technology ◽  
Control Group ◽  
Standard Diet ◽  
Tomato Pomace ◽  
White Rats ◽  
Experimental Group

Для получения препаратов и добавок, обладающих антиоксидантными свойствами, представляют интерес выжимки томатов, образующиеся при производстве сока прямого отжима с предварительным отделением семян. Проведена сравнительная оценка эффективности функциональных свойств образцов пищевой добавки, полученных из выжимок томатов по различным технологиям: образец добавки 1 получен по технологии, включающей ИК-сушку выжимок, их охлаждение и измельчение; образец добавки 2 – по инновационной технологии, включающей дополнительную предварительную обработку выжимок в ЭМП СВЧ перед ИК-сушкой. Исследования проводили в течение 30 дней на трех группах лабораторных белых крыс: контрольной, животные которой получали стандартный рацион, и двух опытных, в которых животные помимо основного рациона получали при кормлении индивидуально 1 раз в сутки 2 г добавки образца 1 (1-я опытная группа) или образца 2 (2-я опытная группа). Установлено, что степень снижения концентрации ферментов печени АсАт и АлАт в крови животных 2-й опытной группы соответственно на 10,6 и 11,0% выше, чем в крови животных 1-й опытной группы. Степень снижения содержания диеновых коньюгатов, кетодиенов и малонового диальдегида в крови животных 2-й опытной группы была соответственно на 22,1; 10,1 и 12,7% выше по сравнению с аналогичными показателями животных 1-й опытной группы. Таким образом, доказано, что пищевая добавка «Томатная», выработанная по инновационной технологии, проявляет ярко выраженные антиоксидантные и гепатопротекторные свойства, что обеспечивает нормализацию биохимических процессов в организме, повышает его антиоксидантную защиту и препятствует разрушению мембран клеток. For the obtaining of preparations and additives with antioxidant properties, tomato pomace, formed during the production of direct-pressed juice with preliminary separation of seeds, is of interest. A comparative evaluation of the effectiveness of the functional properties of food additive samples obtained from tomato pomace using various technologies was carried out. The sample of additive 1 was obtained using a technology that includes IR drying of pomace, cooling and grinding; the sample of additive 2 was obtained using a technology that includes additional pretreatment of pomace in microwave EMF before IR drying. The studies were carried out for 30 days on three groups of laboratory white rats: a control group, the animals of which were given a standard diet, and two experimental ones, in which the animals, in addition to the main diet, were given individually once a day with 2 g of an additive of sample 1 (1st experimental group) or sample 2 (2nd experimental group). It was found that the degree of decrease in the concentration of liver enzymes AsAt and AlAt in the blood of animals of the 2nd experimental group was respectively 10,6 and 11,0% higher, than in the blood of animals of the 1st experimental group. The degree of decrease in the content of diene conjugates, ketodienes and malondialdehyde in the blood of animals of the 2nd experimental group was higher respectively by 22,1; 10,1 and 12,7% compared with similar indicators of animals of the 1st experimental group. Thus, it is proved that the food additive «Tomatnaya», developed by innovative technology, shows pronounced antioxidant and hepatoprotective properties, which ensures the normalization of biochemical processes in the body, increases its antioxidant protection and prevents the destruction of cell membranes.

scholarly journals Vitamin K2 Supplementation Does Not Affect Vitamin K-Dependent Coagulation Factors Activity in Healthy Subjects: A Pilot Study

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1843-1843
Author(s):  
Jing Tan ◽  
Ruijun Ren ◽  
Dan Xu
Keyword(s):  
Vitamin K ◽  
Healthy Subjects ◽  
Coagulation Factors ◽  
Vitamin K2 ◽  
Control Group ◽  
Healthy Population ◽  
Thrombin Time ◽  
Healthy Individuals ◽  
Significant Difference ◽  
Healthy Control

Abstract Objectives Vitamin K is generally regarded as a procoagulant drug with physicians, concerns have been raised about its effects on hemostasis in the healthy population. We aimed to investigate whether vitamin K2 affects activities of individual vitamin K dependent coagulation factors in healthy individuals without anticoagulation treatment. Methods Forty healthy volunteers between 25 and 40 years old were recruited. They received 90 μg of vitamin K2 every day for 30 days. Prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), international normalized ratio (INR), fibrinogen (FIB) levels and blood coagulation factors II, VII, IX, and X activity levels (F II : C, FⅦ : C, FⅨ : C,FⅩ : C), protein induced by vitamin K absence or antagonist-II (PIVKA-II), which is uncarboxylated prothrombin were measured at day 0, and day 30 after vitamin K2 administration. Plasma diluted 1:10 from vitamin K2 group and healthy control group were assayed for the activity of factors II, VII, IX, and X. Results PT, APTT, TT, and FIB did not show significant difference at day 30 when compared with baseline. The activities of coagulation factors II, VII, IX, and X was not significantly different with baseline (97.28 ± 12.42% vs. 99.96 ± 10.24%, P = 0.24 for F II: C; 76.12 ± 15.82% vs. 76.40 ± 12.33%, P = 0.92 for FⅦ: C; 97.65 ± 13.98% vs. 99.65 ± 13.30%, P = 0.47 for FⅨ: C; 89.18 ± 10.76% vs. 92.01 ± 10.46%, P = 0.1 for FⅩ: C) . PIVKA-II levels were not changed with 30 days vitamin K2 supplementation (21.62 ± 3.21 vs. 23.87 ± 2.65 mAU/ml, P = 0.16). After 30 days vitamin K2 administration, factor II, Ⅶ, Ⅸ, and Ⅹ activity of plasma diluted up to 10 times were proportionally decreased, and did not show significant difference with the healthy control without vitamin K2 exposure (10.32 ± 1.24% vs. 10.97 ± 1.55%, P = 0.38 for F II: C; 9.52 ± 2.94% vs. 9.14 ± 1.79%, P = 0.68 for FⅦ: C; 11.78 ± 2.12% vs.11.65 ± 1.54%, P = 0.87 for FⅨ: C; 8.22 ± 1.28% vs. 8.92 ± 1.13%, P = 0.21 for FⅩ: C). Conclusions Vitamin K2 supplementation at recommended dosage does not affect vitamin K-dependent coagulation factors activity in healthy subjects. Uncarboxylated prothrombin (PIVKA-II) in healthy individuals is not decreased with vitamin K supplementation. Funding Sources None.

scholarly journals APPLICATION OF CELLULAR TECHNOLOGIES FOR REGENERATION OF PERIODONTAL TISSUES IN EXPERIMENT

2018 ◽  
Vol 62 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Sergei P. Rubnikovich ◽  
Igor D. Volotovsky ◽  
Yulia L. Denisova ◽  
Тatiana E. Vladimirskaya ◽  
Vasilina A. Andreeva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Morphological Changes ◽  
Fibrous Tissue ◽  
Laboratory Animals ◽  
Control Group ◽  
Cell Transplant ◽  
Periodontal Tissues ◽  
White Rats

A promising scope of modern scientific research is the use of autologous and allogeneic mesenchymal stem cells for regeneration of periodontal tissues. The aim of the study was to evaluate the nature of morphological changes in the pathologically altered periodontal tissues after injection of a biotransplant containing mesenchymal stem cells of the adipose tissue immobilized on a collagen carrier in an animal experiment. In the experiment, 60 randobbred females of white rats were used as a model, whose adipose tissue was taken to obtain allogenic mesenchymal stem cells. All animals were divided into 5 groups, depending on the planned method of treatment – 10 rats each. The control group consisted of 10 laboratory animals with healthy gingiva. The experimental gingival recession model was created by the V-shaped excision of periodontal tissues. The bioplastic collagen material “Collost” gel 7 % in isolated form determines the fibrosis intensification and serves as a “matrix” for the formation of a fibrous tissue, ensures the adhesion of stem cells and their transformation into pro- and fibroblasts. Injection of a cell transplant suspension into physiological saline activates the processes of cell proliferation and transformation of fibroblast differentiating cells. Suspension of the cell transplant on a sterile bioplastic collagen material “Collost” gel 7 % enhances the effects of gel and stem cells, promotes the leveling of atrophic and dystrophic changes in the gum, strengthening a mechanical component, reducing the recession of the gum and the depth of the gingival pocket.

scholarly journals The Effect of Black Garlic (Allium sativum Linn) on Cardiac and Aortic Histopathology in Experimental Studies in Obesity Rats

2021 ◽  
Vol 7 (2) ◽  
pp. 62-68
Author(s):  
Andreas Arie Setiawan ◽  
Fairuz Azmila Purnomo ◽  
Vega Karlowee ◽  
Noor Wijayahadi
Keyword(s):  
Body Weight ◽  
Wall Thickness ◽  
Experimental Studies ◽  
Optimal Dose ◽  
The Body ◽  
Heart Weight ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
White Rats

ABSTRACTBackground: Obesity is a disorder or disease characterized by the accumulation of excess fat in the body due to an imbalance in energy intake that is used for a long time. Accumulation of fat can reduce adiponectin, causing cardiac hypertrophy, endothelial vasodilation, and other cardiovascular diseases. Black garlic have high antioxidants in the form of S-Allylcysteine(SAC) which functions to increase adiponectin. Objective: To determine the effect of Black garlic on the histopathological picture of the heart and aorta of obese rats. Methods: This study was an experimental study with a randomized post-test only design with control group design with 5 groups of male white rats Sprague Dawley (Rattus novergicus) fed High Fat Fructose. Diet (HFFD) enriched with 1.25% cholesterol and 0.5% cholic acid for 8 weeks and was given black garlic intervention at doses of 450 mg / 200BW, 900mg / 200BW and 1350mg200 / BW for 4 weeks. Results: Giving black garlic significantly reduced body weight of rats (p = 0.001), and the results did not significantly reduce heart weight (p = 0.147), aortic weight (p = 0.061), histopathological changes in heart wall thickness (p = 0.423) and aortic wall thickness (p = 0.802). The effective doses of black garlic in this study were 450 mg / 200 grams BW, 900 mg / 200 grams rat BW and 1350 mg / 200 grams BW of rats. The optimal dose is 900 mg / 200 grams BW. Conclusion: Black garlic gave a significant reduction in body weight of rats and no significant reduction in heart weight, aortic weight, cardiac and aortic histopathological features. 

scholarly journals Adaptation disorders caused by nephrectomy

2019 ◽  
Vol 9 (2) ◽  
pp. 23-28
Author(s):  
Igor S. Shormanov ◽  
Marina S. Los ◽  
Maxim V. Kosenko
Keyword(s):  
Adaptive Response ◽  
Immobilization Stress ◽  
Negative Impact ◽  
Integrated Approach ◽  
The Body ◽  
Laboratory Animals ◽  
Clinical Markers ◽  
White Rats ◽  
Wide Range ◽  
Significant Negative Impact

Currently, nephrectomy is performed for various diseases, and the number of this type of surgery does not have a clear tendency to decrease. Operational stress is accompanied by a triggering mechanism for a wide range of disorders, among which psycho-emotional and vegetative (vascular) reactions are important clinical markers. The study of the systemic adaptive reactivity of the body after nephrectomy will provide an integrated approach to the study of the problem of the postoperative period and the rehabilitation of the patient in clinical practice. Objective: to identify the effect of nephrectomy on the overall adaptive response of animals when white rats are exposed to damaging physical loads (acute hyperthermia) and chemical (acute alcohol poisoning, nephrotoxic agents) of nature, normobaric hypoxia, limiting muscular loads and immobilization stress. The results of the experiment showed that laboratory animals with a single kidney become more sensitive (1.5 times) to nephrotoxic agents. Analysis of the effect of nephrectomy on the overall adaptive response of animals under the influence of various damaging factors to reduce the body’s resistance to hypoxia by 20% (p > 0.05); to limit muscular loads – by 25% (p > 0.05); hyperthermia – by 25% (p < 0.05); chemical agents – by one third (p < 0.05); to immobilization stress – by 28% (p < 0.05). Conclusion. The study demonstrated a significant negative impact of nephrectomy on the overall adaptive capacity of the body and allowed to clarify the functional characteristics of the general adaptation disorders in connection with this operation.

scholarly journals ACUTE CHRONICAL TOXICITY AND INTOLERANCE OF SM - COMPLEX

2019 ◽  
pp. 96-103
Author(s):  
A. V. Mifatkhudinov ◽  
A. S. Aleshina
Keyword(s):  
Therapeutic Dose ◽  
Healthy Adult ◽  
Morphological Changes ◽  
The Body ◽  
Control Group ◽  
Pharmacological Substance ◽  
High Doses ◽  
Therapeutic Doses ◽  
Permissible Dose

The researchers elaborated pharmacological complex of SM at the Chair of Morphology, Physiology and Pharmacology atSouth-UralStateAgrarianUniversity. The complex contains butafosfan, vitamins, vitamin-like substances, selected on the basis of synergistic effect on the body. The parameters of acute toxicity of the SM-complex were explored in the experiment on clinically healthy adult white mice of both genders by a single injection of the solution in the maximum permissible dose according to GOST 31926-2013. Intolerance on the skin of animals was detected in line with GOST R ISO 10993.10-2009. GOST R ISO 10993.10-99 became a guideline for evaluating SM-complex intolerance on the eye conjunctiva. Chronic toxicity was investigated on non-linear rats; the rates were divided into 4 groups of 6. The first group became a control group, the second group received the SM-complex in a therapeutic dose (255mg/kg), the third group - in a 5 -fold therapeutic dose (1275mg/kg), the fourth group - in a 10-fold dose (2550mg/kg). Pharmacological substance was applied through the probe in the form of an aqueous solution for 30 days. The researchers found out that at single oral introduction of SM-complex in the maximum possible doses it does not affect mice organism and it is referred to the 4th class of danger according to GOST 12.1.007-76. Local application in the form of applications on the skin and mucous membranes of rabbits, the complex does not have a local irritant effect. Long-term application of pharmacological composition (30 days) in high doses causes functional and morphological changes of the liver in the form of gray foci and flabbiness, as well as it increases the volume of the organ. Due to the fact that the therapeutic doses are 5 and 10 times lower than the toxic ones and the period of application does not exceed 7-14 days, the authors make a conclusion that SM-complex is safe and secure and can be used in the recommended doses.

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