scholarly journals Evaluation of Properties of Dikiri Pulp in the Formulation of Jam

CORD ◽  
2015 ◽  
Vol 31 (2) ◽  
pp. 7
Author(s):  
K.D.P.P. Gunathilake
Keyword(s):  
Room Temperature ◽  
Dry Weight ◽  
Weight Basis ◽  
Composition Analysis ◽  
Life Study ◽  
Sensory Acceptability ◽  
Pectin Content ◽  
Kernel Composition ◽  
Keeping Quality ◽  
Pineapple Pulp

The peculiar farm of coconut,” Dikiri” was studied for its kernel composition including pectin content with the objective of developing a jam out of Dikiri kernel. Three levels of Dikiri pulp, 20%, 30% and 40%, were evaluated to prepare Dikiri based jam. Two types of jam were prepared; Jam made with Dikiri only as the fruit pulp and the jam with Dikiri pulp together with 10% pineapple. They were tested for sensory attributes and were compared with pineapple jam prepared with added commercial high methoxyl pectin. The best jam was selected for shelf life study at room temperature. Results showed that Dikiri kernel differs from ordinary coconut for all the variables tested in proximate composition analysis. Dikiri kernel contained 16.6% of high methoxyl pectin on a dry weight basis. The jam with 30% Dikiri pulp was selected as the best total pulp ratio and the incorporation of 10% pineapple pulp into Dikiri gives better sensory properties compared with Dikiri only jam.  In conclusion, there is a possibility of formulation of jam without adding external pectin with considerable sensory acceptability and good keeping quality.

scholarly journals Chemical Composition of Essential Oil from Dalbergia odorifera Flowers and HPLC Analysis of Tectorigenin in Its Leaves and Branches

2021 ◽  
Vol 25 (03) ◽  
pp. 723-729
Author(s):  
Ziling Mao
Keyword(s):  
Chemical Composition ◽  
High Performance ◽  
Dry Weight ◽  
Volatile Oil ◽  
Weight Basis ◽  
Composition Analysis ◽  
Dalbergia Odorifera ◽  
Main Components ◽  
First Time ◽  
Further Development

Dalbergia odorifera T. Chen, as an important traditional Chinese medicinal plant, has been used in China over a long history. The chemical composition of volatile oil extracted from the D. odorifera flowers is described for the first time here. The volatile oil was extracted by hydro-distillation, and GC-MS was used for the chemical composition analysis. Tectorigenin, an isoflavonoid, was also isolated from the flowers. The structure of tectorigenin was established based on 1H and 13C NMR and HR-ESI-MS spectrometry. The main components of the volatile oil from the flowers were 4-hydroxy-4-methyl-2-pentanone (28.35%), phenethyl alcohol (12.17%), cis-5-ethenyltetrahydro-α, α-5-trimethyl-2-furanmethanol (8.71%), toluene (7.64%), p-xylene (5.93%), benzyl alcohol (5.72%) and ethylbenzene (5.35%). The tectorigenin contents in the xylem, phloem and leaves were determined by high-performance liquid chromatography (HPLC) as 75.44 μg/g, 104.26 μg/g and 393.11 μg/g, respectively, on a dry weight basis and 49.32 μg/g, 51.98 μg/g and 74.45 μg/g, respectively, on a fresh weight basis. The study provides an important theoretical basis for the further development and application of the D. odorifera flowers and tectorigenin. © 2021 Friends Science Publishers

ERRORS ASSOCIATED WITH HERBAGE DISSECTION ANALYSES 2. Problems of species identification

1976 ◽  
pp. 213-225
Author(s):  
I.M. Ritchie ◽  
C.C. Boswell ◽  
A.M. Badland
Keyword(s):  
New Zealand ◽  
Dry Weight ◽  
Analytical Tool ◽  
Species Level ◽  
Weight Basis ◽  
Plant Identification ◽  
Leaf Fragment ◽  
Research Division ◽  
Or Groups ◽  
Botanical Analysis

HERBACE DISSECTION is the process in which samples of herbage cut from trials are separated by hand into component species. Heavy reliance is placed on herbage dissection as an analytical tool ,in New Zealand, and in the four botanical analysis laboratories in the Research Division of the Ministry of Agriculture and Fisheries about 20 000 samples are analysed each year. In the laboratory a representative subsample is taken by a rigorous quartering procedure until approximately 400 pieces of herbage remain. Each leaf fragment is then identified to species level or groups of these as appropriate. The fractions are then dried and the composition calculated on a percentage dry weight basis. The accuracy of the analyses of these laboratories has been monitored by a system of interchanging herbage dissection samples between them. From this, the need to separate subsampling errors from problems of plant identification was, appreciated and some of this work is described here.

Polycyclic Aromatic Hydrocarbons (PAHs) in Sediments of the Brisbane River (Australia) – Preliminary Results

1989 ◽  
Vol 21 (2) ◽  
pp. 161-165 ◽  
Author(s):  
S. I. Kayal ◽  
D. W. Connell
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
River Estuary ◽  
Dry Weight ◽  
Weight Basis ◽  
Sediment Samples ◽  
Preliminary Results ◽  
Treated Sewage ◽  
Polycyclic Aromatic ◽  
Petroleum Refineries

Results of the analysis of twenty-three composite sediment samples revealed that PAHs are widely distributed in the Brisbane River estuary. Mean concentrations for individual compounds, on a dry weight basis, ranged from 0.03 µg/g for dibenz [ah] anthracene to 2.34 µg/g for fluoranthene. Observed PAH assemblages were rich in compounds having pyrolytic origins. However, the presence of petroleum derived compounds was indicative of the importance of petroleum as a PAH source in the estuary. Petroleum refineries, a coal loading terminal and a major treated sewage outfall located at the mouth were not indicated as major contributing sources of PAH pollution in the estuary.

Mineral nutrition, dinitrogen fixation, and growth of Alnuscrispa and Alnusglutinosa

1985 ◽  
Vol 15 (5) ◽  
pp. 855-861 ◽  
Author(s):  
G. Prégent ◽  
C. Camiré
Keyword(s):  
Mineral Nutrition ◽  
Dry Weight ◽  
Optimal Growth ◽  
Maximum Growth ◽  
Dinitrogen Fixation ◽  
Weight Basis ◽  
Critical Levels ◽  
N Status

Invitro cultures of Alnuscrispa (Ait.) Pursh and Alnusglutinosa (L.) Gaertn. were used to estimate critical foliage levels of selected nutrients for optimal growth and dinitrogen (N2) fixation. For A. crispa to obtain 90% of maximum growth and N2 fixation, foliar levels of 0.12% P, 0.13% Mg, <0.31% K, and <0.04% Ca on a dry weight basis were needed. For A. glutinosa, the critical levels were 0.138% P, 0.10% Mg, 0.29% Ca, and ~0.20% K. From all the deficiencies observed, P had the more pronounced effects on N status of both species.

SOLUBLE SUGAR CHANGES OCCURRING DURING COLD HARDENING OF SPRING WHEAT, FALL RYE AND ALFALFA

1983 ◽  
Vol 63 (2) ◽  
pp. 415-420 ◽  
Author(s):  
D. G. GREEN
Keyword(s):  
Spring Wheat ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Weight Basis ◽  
Cold Hardening ◽  
Spring Type

Alfa, a relatively nonhardy alfalfa cultivar continued to accumulate, on a dry weight basis, fructose, α- and β-D-glucose, sucrose and maltose during the latter stages of cold hardening. Rambler, a hardier alfalfa cultivar conversely showed a decrease for these soluble sugars with hardening. Frontier rye, a very hardy winter habit cereal showed decreases in these soluble sugars plus melibiose during the same hardening period. These results support the hypothesis that hardy cereals and alfalfa undergo a decrease in soluble sugars with hardening, while less hardy cereals and alfalfa continue to increase in content of soluble sugars. Manitou wheat appeared not to fit this hypothesis and showed the decreased soluble sugars usually associated with hardy cultivars. Although Manitou is a spring type wheat, one of its parents, Thatcher, does contain gene(s) for the winter habit.Key words: Sugar, cold hardening, wheat, rye, alfalfa

scholarly journals THE MOLECULAR WEIGHT OF RHODOPSIN AND THE NATURE OF THE RHODOPSIN-DIGITONIN COMPLEX

1954 ◽  
Vol 37 (3) ◽  
pp. 381-399 ◽  
Author(s):  
Ruth Hubbard
Keyword(s):  
Molecular Weight ◽  
Single Molecule ◽  
Extinction Coefficient ◽  
Dry Weight ◽  
Weight Basis ◽  
Sedimentation Behavior ◽  
A Value ◽  
Wet Weight ◽  
Single Boundary ◽  
Stoichiometric Complex

The sedimentation behavior of aqueous solutions of digitonin and of cattle rhodopsin in digitonin has been examined in the ultracentrifuge. In confirmation of earlier work, digitonin was found to sediment as a micelle (D-1) with an s20 of about 6.35 Svedberg units, and containing at least 60 molecules. The rhodopsin solutions sediment as a stoichiometric complex of rhodopsin with digitonin (RD-1) with an s20 of about 9.77 Svedberg units. The s20 of the RD-1 micelle is constant between pH 6.3 and 9.6, and in the presence of excess digitonin. RD-1 travels as a single boundary also in the electrophoresis apparatus at pH 8.5, and on filter paper at pH 8.0. The molecular weight of the RD-1 micelle lies between 260,000 and 290,000. Of this, only about 40,000 gm. are due to rhodopsin; the rest is digitonin (180 to 200 moles). Comparison of the relative concentrations of RD-1 and retinene in solutions of rhodopsin-digitonin shows that RD-1 contains only one retinene equivalent. It can therefore contain only one molecule of rhodopsin with a molecular weight of about 40,000. Cattle rhodopsin therefore contains only one chromophore consisting of a single molecule of retinene. It is likely that frog rhodopsin has a similar molecular weight and also contains only one chromophore per molecule. The molar extinction coefficient of rhodopsin is therefore identical with the extinction coefficient per mole of retinene (40,600 cm.2 per mole) and the E(1 per cent, 1 cm., 500 mµ) has a value of about 10. Rhodopsin constitutes about 14 per cent of the dry weight, and 3.7 per cent of the wet weight of cattle outer limbs. This corresponds to about 4.2 x 106 molecules of rhodopsin per outer limb. The rhodopsin content of frog outer limbs is considerably higher: about 35 per cent of the dry weight, and 10 per cent of the wet weight, corresponding to about 2.1 x 109 molecules per outer limb. Thus the frog outer limb contains about five hundred times as much rhodopsin as the cattle outer limb. But the relative volumes of these structures are such that the ratio of concentrations is only about 2.5 to 1 on a weight basis. Rhodopsin accounts for at least one-fifth of the total protein of the cattle outer limb; for the frog, this value must be higher. The extinction (K500) along its axis is about 0.037 cm.2 for the cattle outer limb, and about 0.50 cm.2 for the frog outer limb.

Influence of Temperature and Water Activity on Aflatoxin Production by Aspergillus flavus in Cowpea (Vigna unguiculata) Seeds and Meal

1985 ◽  
Vol 48 (12) ◽  
pp. 1040-1043 ◽  
Author(s):  
P. E. KOEHLER ◽  
L. R. BEUCHAT ◽  
M. S. CHHINNAN
Keyword(s):  
Heat Treatment ◽  
Water Activity ◽  
Aspergillus Flavus ◽  
Vigna Unguiculata ◽  
Dry Weight ◽  
Aflatoxin Production ◽  
Weight Basis ◽  
Influence Of Temperature

Experiments were done to determine the influence of temperature (21, 30 and 37°C) and aw (0.76 to 0.98) on aflatoxin production by Aspergillus flavus on cowpea (Vigna unguiculata) seeds, meal and meal supplemented with onion. Larger quantities of aflatoxin were produced at 21 and 30°C than at 37°C. The highest amount of aflatoxin (2777 μg/20 g, dry weight basis) was observed in meal containing onion at aw 0.98 after 20 d of incubation at 21°C. A level of 870 |μg/20 g was detected in seeds at aw 0.95 after 14 d of incubation at 30°C. Meal at aw 0.96 supported production of 551 μg of aflatoxin per 20 g after 20 d at 30° C. Temperature had little influence on the optimal aw for aflatoxin production in cowpea meal. However, an increase in temperature resulted in a decreased optimal aw for aflatoxin production on whole cowpeas. When known quantities of aflatoxin were added to cowpea meal which was subsequently steamed for 5 min, only 29% was extractable using a variety of procedures, indicating that the toxin may be bound in some manner to cowpea constituents as a result of heat treatment.

scholarly journals An Alternative Coupling Procedure for Preparing Activated Sepharose for Affinity Chromatography of Penicillinase

1976 ◽  
Vol 29 (4) ◽  
pp. 305 ◽  
Author(s):  
RG Coombe ◽  
AM George
Keyword(s):  
Affinity Chromatography ◽  
Room Temperature ◽  
Cyanogen Bromide ◽  
Dry Weight ◽  
Enzymic Activity ◽  
Covalent Attachment ◽  
Affinity Column ◽  
Coupling Procedure ◽  
Coupling Techniques ◽  
Cyanogen Bromide Activation

Most applications of affinity chromatography employ the cyanogen bromide activation scheme first devised by Axtm et al. (1967). Porath and Sundberg (1972) reported an alternative procedure in which phloroglucinol and divinylsulphone are used in activating reactions. The advantages of this scheme and parameters relevant to the activating reactions are reported here. Conditions for the attachment of various ligand molecules to sepharose using a divinylsulphone activation method are defined, and a comparison with cyanogen bromide activating and coupling techniques is drawn. a-Chymotrypsin is immobilized by covalent attachment to activated sepharose. The optimum coupling pH is 8� 0-8� 6 and the reaction is virtually complete after 20 h at room temperature. Conjugates containing as much as 2 g of enzyme per gram dry weight of polymer were obtained. The immobilized enzyme retained 41 % of the free enzymic activity. An affinity column of divinylsulphone-activated methicillin-sepharose was used to demonstrate the reversible adsorption of penicillinase.

Low K+ in Paphiopedilum leeanum leaf epidermis: implications for stomatal functioning

1977 ◽  
Vol 55 (4) ◽  
pp. 489-495 ◽  
Author(s):  
Sherman D. Nelson ◽  
James M. Mayo
Keyword(s):  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Weight Basis ◽  
Leaf Epidermis ◽  
Photometric Analysis ◽  
Abaxial Epidermis ◽  
Absorption Spectrophotometry ◽  
Stomatal Functioning ◽  
Low K ◽  
Stomatal Mechanism

Abaxial epidermal strips from leaves of Paphiopedilum leeanum were analyzed via sodium cobaltinitrite staining and atomic absorption spectrophotometry for the presence and location of potassium. On a dry weight basis K content of the abaxial epidermis was found to be 103 times less than has been reported in other species, and unlike other species no localization of K+ in guard cells of open stomata could be detected via the sodium cobaltinitrite stain for potassium.Flame photometric analysis of the mesophyll indicated that it contained normal amounts of K+ (about 1.87% on a dry weight basis). Analysis showed that the K+ content of the abaxial epidermis (0.032%) was considerably less than that of the mesophyll, a situation unlike previous reports for other species in which the epidermal concentration was found to be greater than the mesophyll. A process for exclusion of K+ from the abaxial epidermis is suggested, as is the lack of involvement of K+ as the major osmoticum in the stomatal mechanism of this species.

scholarly journals Studies on the Preservation of Raw Cow’s Milk by Chemical Method

1970 ◽  
Vol 42 (3) ◽  
pp. 317-326 ◽  
Author(s):  
F Rokhsana ◽  
UK Das ◽  
R Yeasmin ◽  
A Nahar ◽  
S Parveen
Keyword(s):  
Hydrogen Peroxide ◽  
Chemical Method ◽  
Room Temperature ◽  
Storage Period ◽  
Total Coliform ◽  
Human Consumption ◽  
Milk Products ◽  
E Coli ◽  
Keeping Quality ◽  
Control Milk

Studies carried out to develop a technique for the preservation of cow's milk in raw condition using hydrogen peroxide (H2O2) as a preservative. Fresh cow’s milk was collected and experiments were conducted by four treatments in order to achieve the optimum condition of storage. The treatments were with various concentration of H2O2 starting from 0.05 %, 0.1 %, 0.2 %, 0.3 %, 0.4 %, & 0.5 %. Treated milk with 0.05 % concentration of H2O2 had storage period of 20 days compared to that of the control one (5 days only) in refrigerated temperature (±8°C). On the other hand hydrogen peroxide treated milk (0.05 %) had a storage period of 8 hours at room temperature (±28°C). Results also showed that the higher concentration of H2O2 had no effect on storage period than that of control. Milk products like kheer and halawa prepared by treated milk and stored for 20 days showed almost nil growth of total coliform and E. coli which means that food products prepared from hydrogen peroxide treated milk is safe for human consumption. Key words: Raw, Storage, Hydrogen peroxide, Preservative, keeping quality, Pasteurization, deteriorated, MPN. Bangladesh J. Sci. Ind. Res. 42(3), 317-326, 2007

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