Increasing Atherosclerosis in Streptozotocin-Induced Diabetes into Four Groups of Mice

AIM: To study the protective effect of medicines on the formation of atherosclerosis in mice, it is needed to conduct the study in mice which is not genetically diabetic mice induced by streptozotocin (STZ) to produce hyperglycemia and atherosclerosis, compared with mice treated by yolk or its combination.MATERIAL AND METHODS: Fifty-six mice, Double Deutch Webster strain, male, receive 10 weeks, 20 - 30 gr bodyweight were divided into 4 groups (n = 14) i.e. control (do not received any agents), STZ (45 mg/kg/BW was injected intraperitoneally for 5 days), yolk (0.2 cc orally daily for 6 weeks), and combination of STZ and yolk (STZ: 45 mg/kg/BW intraperitoneally add 0.2 cc yolk orally). All animals were executed in the 42nd day. Then, the aorta of the mice’s heart tissue was histopathology examined. Blood glucose and cholesterol levels were determined every week.RESULTS: Hyperglycemia occurred in mice induced by STZ injection with the highest BGL (521.8 ± 48.2 mg/dl; 188.4%) in the 4th-week observation; after that BGL decrease. We found that, except the control, all treatment groups with STZ, egg yolk, and combination underwent atherosclerosis.CONCLUSION: The present study was able to demonstrate the occurrence of atherosclerosis in mice treated by STZ accompanied with increasing blood glucose and cholesterol level.

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Effects of Malted Barley Extract and Banaba Extract on Blood Glucose Levels in Genetically Diabetic Mice

Journal of Medicinal Food ◽

10.1089/jmf.2004.7.487 ◽

2004 ◽

Vol 7 (4) ◽

pp. 487-490 ◽

Cited By ~ 19

Author(s):

Heeok Hong ◽

Won Jai Maeng

Keyword(s):

Blood Glucose ◽

Diabetic Mice ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Genetically Diabetic Mice

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UJI PENURUNAN KOLESTEROL PADA MENCIT PUTIH (Mus musculus) SECARA IN-VIVO MENGGUNAKAN EKSTRAK METANOL UMBI TALAS (Colocasia esculenta L) SEBAGAI UPAYA PENCEGAHAN CARDIOVASCULAR DISEASE

JURNAL PIJAR MIPA ◽

10.29303/jpm.v11i2.113 ◽

2016 ◽

Vol 11 (2) ◽

Cited By ~ 1

Author(s):

Samsul Rizal Umami ◽

Sarifa Siti Hapizah ◽

Rosita Fitri ◽

Aliefman Hakim

Keyword(s):

Mus Musculus ◽

Broiler Chicken ◽

Egg Yolk ◽

Extraction Methods ◽

Colocasia Esculenta ◽

Root Extract ◽

Treatment Groups ◽

Cholesterol Levels ◽

Better Than

Abstrak : Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak umbi talas (Colocasia esculenta L) terhadap penurunan kadar kolesterol mencit putih (Mus musculus). Induksi hiperkolesterolemik dilakukan dengan pemberian kuning telur puyuh, pakan ayam broiler dan pemberian propiltiourasil (PTU) pada mencit putih (Mus musculus). Penelitian ini menggunakan metode ekstraksi dan uji penurunan kadar kolesterol dengan ekstrak umbi talas. Mencit dibagi menjadi 5 kelompok percobaan yang terdiri dari 3 kelompok perlakuan (P1, P2, dan P3) dan 2 kelompok kontrol (K+ dan K-). Dosis yang digunakan yaitu 100 mg/kgBB, dosis P2 yaitu 200 mg/kgBB, dan P3 yaitu 300 mg/kgBB. Sedangkan kelompok K+ diberikan dosis simvastatin dan K- hanya aquades. Hasil penelitian menunjukkan bahwa pada dosis 100 mg/kgBB terjadi peningkatan kadar kolesterol sebesar 5,2 mg/dL, pada K- juga mengalami peningkatan sebesar 3,8 mg/dL. Sedangkan pada P2 mengalami penurunan yang cukup tinggi sebesar 27,9 mg/dL, sementara P3 mengalami penurunan sebesar 2,4 mg/dL dan K+ juga mengalami penurunan sebesar 2,2 mg/dL. Berdasarkan hasil ini, dosis 200 mg/kgBB menunjukkan penurunan dosis yang lebih baik daripada dosis yang lain. Kata Kunci : Hiperkolesterolemik, PTU, Umbi Talas, Ekstraksi, Kolesterol Abstrack: This study aimed to determine the effect of taro root extract (Colocasia esculenta L) to decrease cholesterol levels of white mice (Mus musculus). Induction hiperkolesterolemik done by providing quail egg yolk, broiler chicken feed and administration of propylthiouracil (PTU) in white mice (Mus musculus). In this study using extraction methods and test cholesterol-lowering with taro root extract. The mice were divided into 5 experimental groups consisting of 3 treatment groups (P1, P2, and P3) and two control groups (K + and K-). In the group P1 doses used were 100 mg / kg body weight, P2 dose of 200 mg / kg, and P3 is 300 mg / kg. While the group K + and K- simvastatin dose given only distilled water. The results showed that at a dose of 100 mg / kg showed elevated levels of cholesterol by 5.2 mg / dL, the K- increased by 3.8 mg / dL. While at P2 decreased quite high by 27.9 mg / dL, P3 decreased by 2.4 mg / dL and K + also decreased by 2.2 mg / dL. Based on these results, a dose of 200 mg / kg showed a dose reduction is better than another dose. Keywords: Hiperkolesterolemik, PTU, Bulbs Talas, Extraction, Cholesterol

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Protective Effect of Ipomoea batatas L Leaves Extract on Histology of Pancreatic Langerhans Islet and Beta Cell Insulin Expression of Rats Induced by Streptozotocin

Molekul ◽

10.20884/1.jm.2020.15.1.563 ◽

2020 ◽

Vol 15 (1) ◽

pp. 48

Author(s):

Dody Novrial ◽

Soebowo Soebowo ◽

Parno Widjojo

Keyword(s):

Blood Glucose ◽

Protective Effect ◽

Ipomoea Batatas ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Sprague Dawley ◽

Control Groups ◽

Protection Effect ◽

Treatment Groups ◽

Sprague Dawley Rats

Sweet potato (Ipomoea batatas L) leaf is one of well known vegetables among Indonesian people. It is also often used as traditional medicine for diabetes mellitus. This research aimed to investigate the protective effect of I. batatas L leaves extract on the pancreas of IDDM animal model. Twenty five male Sprague-Dawley rats were divided into 3 treatment groups, and 2 control groups. Streptozotocin (STZ) was injected at multiple low doses (40 mg/kg BW) intraperitoneally for 5 consecutive days, I. batatas L leaves extract (doses 0.25, 0.8, and 2.5 g/ kg BW) were administered for 14 days after the first STZ injection. Fasting blood glucose was analyzed after complete STZ induction (day 6), and after 14 days treatment. At the end of the study, rats were terminated, and pancreas were removed for histological examination and immunohistochemical procedure using anti-insulin antibody. Diabetic rats treated with 2.5 g/kg BW I. batatas L leaves extract showed lowest fasting blood glucose among treatment groups, and had approximately 50% normal Langerhans islets with functional beta cells. These results suggest that I. batatas L leaves extract has anti diabetic activity through its protection effect on the pancreas.

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Oxidative Effects in Streptozotocin-induced Male and Female Mice: The Effect of Garlic Oil and Melatonin

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i58a34124 ◽

2021 ◽

pp. 336-343

Author(s):

Abeer Al-Rashedi ◽

Banan Al-Johari ◽

Rahaf Al-Abbasi ◽

Raghdh Sindi ◽

Rawan Bin Hameed ◽

...

Keyword(s):

Oxidative Stress ◽

Uric Acid ◽

Blood Glucose ◽

Fasting Blood Glucose ◽

Lipid Peroxide ◽

Lipid Peroxides ◽

Diabetic Mice ◽

Garlic Oil ◽

Streptozotocin Induced Diabetes ◽

Oxidative Effects

Background: Recent studies have revealed that a hyperglycemia-induced overproduction of superoxide can be the first event in the activation of all pathways involved in the pathogenesis of complications of diabetes. Supplementation of garlic was found to decrease diabetes-induced oxidative stress complications. Studies shown also that melatonin attenuates diabetes‐induced oxidative stress in diabetic induced rabbits and rats. Objective: In this present study, oxidative stress in diabetic model and the effect of garlic oil or melatonin treatment were examined in both genders' male and females' mice. Methods: 96 mice were randomly divided into 5 groups including control (C), diabetic (D), melatonin 10 mg/kg (D+M), garlic extract 100 mg/kg (D+G) and combined melatonin and garlic (D+M+G). All treatments were given orally daily for 16 weeks after induction of hyperglycemia by streptozocin (STZ). Fasting blood glucose and antioxidant levels were estimated. Results: Streptozotocin induced diabetic mice, showed a significant increase of plasma glucose, lipid peroxide and uric acid. Accordingly, significant decreases in the levels of antioxidants ceruloplasmin were found in the plasma of diabetic mice. Treatment of diabetic mice with garlic oil or melatonin for 16 weeks significantly increased plasma levels of ceruloplasmin activities. Lipid peroxides, uric acid, blood glucose was decreased significantly after treatment with garlic oil or melatonin. Conclusion: The results suggest that garlic oil or melatonin may effectively normalize the impaired antioxidants status in streptozotocin induced diabetes in both males and females mice.

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Antidiabetic and Antihyperlipidemic Effects of Sulphurenic Acid, a Triterpenoid Compound from Antrodia camphorata, in Streptozotocin-Induced Diabetic Mice

International Journal of Molecular Sciences ◽

10.3390/ijms20194897 ◽

2019 ◽

Vol 20 (19) ◽

pp. 4897 ◽

Cited By ~ 1

Author(s):

Cheng-Hsiu Lin ◽

Li-Wei Hsiao ◽

Yueh-Hsiung Kuo ◽

Chun-Ching Shih

Keyword(s):

Fatty Acid ◽

Blood Glucose ◽

Protective Effect ◽

Fatty Acid Synthase ◽

Control Group ◽

Mrna Levels ◽

Diabetic Mice ◽

Antrodia Camphorata ◽

Expression Levels ◽

Lower Blood

The present study was designed to evaluate the protective effect of sulphurenic acid (SA), a pure compound from Antrodia camphorata, on diabetes and hyperlipidemia in an animal model study and to clarify the underlying molecular mechanism. Diabetes was induced by daily 55 mg/kg intraperitoneal injections of streptozotocin (STZ) solution over five days. Diabetic mice were randomly divided into six groups and orally gavaged with SA (at three dosages) or glibenclamide (Glib), fenofibrate (Feno) or vehicle for 3 weeks. Our findings showed that STZ-induced diabetic mice had significantly increased fasting blood glucose, glycated hemoglobin (HbA1C), plasma triglyceride (TG), and total cholesterol (TC) levels (p < 0.001, p < 0.001, p < 0.001, and p < 0.05, respectively) but decreased blood insulin, adiponectin, and leptin levels compared to those of the control group (p < 0.001, p < 0.001, and p < 0.001, respectively). Administration of SA to STZ-induced diabetic mice may lower blood glucose but it increased the insulin levels with restoration of the size of the islets of Langerhans cells, implying that SA protected against STZ-induced diabetic states within the pancreas. At the molecular level, SA treatment exerts an increase in skeletal muscle expression levels of membrane glucose transporter 4 (GLUT4) and phospho-Akt to increase the membrane glucose uptake, but the mRNA levels of PEPCK and G6Pase are decreased to inhibit hepatic glucose production, thus leading to its hypoglycemic effect. Moreover, SA may cause hypolipidemic effects not only by enhancing hepatic expression levels of peroxisome proliferator-activated receptor α (PPARα) with increased fatty acid oxidation but also by reducing lipogenic fatty acid synthase (FAS) as well as reducing mRNA levels of sterol regulatory element binding protein (SREBP)1C and SREBP2 to lower blood TG and TC levels. Our findings demonstrated that SA displayed a protective effect against type 1 diabetes and a hyperlipidemic state in STZ-induced diabetic mice.

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Repeated electroconvulsive shock normalizes blood glucose levels in genetically obese mice (C57BL/6J ob/ob) but not in genetically diabetic mice (C57BL/KsJ db/db)

Brain Research ◽

10.1016/0006-8993(88)91281-4 ◽

1988 ◽

Vol 448 (2) ◽

pp. 377-380 ◽

Cited By ~ 5

Author(s):

David J. Nutt ◽

Christoph H. Gleiter ◽

Markku Linnoila

Keyword(s):

Blood Glucose ◽

Electroconvulsive Shock ◽

Diabetic Mice ◽

Obese Mice ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Genetically Diabetic Mice

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Rasyona Kakule Tozu İlavesinin Yumurtlayan Bıldırcınlarda Performans, Yumurta Kalitesi ve Serum Biyokimyasal Parametrelerine Etkisi

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i11.1999-2003.4635 ◽

2021 ◽

Vol 9 (11) ◽

pp. 1999-2003

Author(s):

Osman Olgun ◽

Esra Tuğçe Gül ◽

Alpönder Yıldız ◽

Abdullah Çolak

Keyword(s):

Serum Cholesterol ◽

Feed Intake ◽

Egg Quality ◽

Egg Yolk ◽

Quality Parameters ◽

Treatment Groups ◽

Cholesterol Levels ◽

A Value ◽

L Value ◽

Different Levels

The present research was carried out to determine the effects of the addition of cardamom powder at different levels (0, 1, 2, 3 or 4 g/kg) to diet on performance, egg quality, and serum parameters in laying quails. In the experiment, a total of 120 laying quails at the age of 20 weeks were randomly distributed to 5 treatment groups with 6 subgroups (4 quails each). Quails were fed with experimental diets for 10 weeks. In the experiment, the feed intake of quails significantly increased with the supplementation of cardamom powder at the level of 3 g/kg to the diet, while other performance parameters were not affected. Eggshell thickness advanced with the addition of 1 g/kg cardamom powder to diet. Egg yolk L* value increased at the level of 4 g/kg, but a* value decreased with the addition of cardamom powder. Other egg quality parameters were not affected by the addition of cardamom powder to the diet. In addition, the supplementation of cardamom powder to the diet significantly reduced serum cholesterol levels of quails. According to the results obtained from the experiment, the addition of cardamom powder at the level of 3 g/kg to the diet increased the feed intake of quails, while the supplementation of 1 g/kg was effective in improving the eggshell quality and serum cholesterol level.

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Anti-Diabetic Activity of a Mineraloid Isolate, in vitro and in Genetically Diabetic Mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000048 ◽

2011 ◽

Vol 81 (1) ◽

pp. 34-42 ◽

Cited By ~ 1

Author(s):

Joel Deneau ◽

Taufeeq Ahmed ◽

Roger Blotsky ◽

Krzysztof Bojanowski

Keyword(s):

Dna Microarrays ◽

Human Fibroblasts ◽

Diabetic Animal ◽

In Vitro Tests ◽

Diabetic Mice ◽

Fossil Material ◽

Expression Of Genes ◽

Enhanced Expression ◽

Genetically Diabetic Mice

Type II diabetes is a metabolic disease mediated through multiple molecular pathways. Here, we report anti-diabetic effect of a standardized isolate from a fossil material - a mineraloid leonardite - in in vitro tests and in genetically diabetic mice. The mineraloid isolate stimulated mitochondrial metabolism in human fibroblasts and this stimulation correlated with enhanced expression of genes coding for mitochondrial proteins such as ATP synthases and ribosomal protein precursors, as measured by DNA microarrays. In the diabetic animal model, consumption of the Totala isolate resulted in decreased weight gain, blood glucose, and glycated hemoglobin. To our best knowledge, this is the first description ever of a fossil material having anti-diabetic activity in pre-clinical models.

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