UJI PENURUNAN KOLESTEROL PADA MENCIT PUTIH (Mus musculus) SECARA IN-VIVO MENGGUNAKAN EKSTRAK METANOL UMBI TALAS (Colocasia esculenta L) SEBAGAI UPAYA PENCEGAHAN CARDIOVASCULAR DISEASE

2016 ◽  
Vol 11 (2) ◽  
Author(s):  
Samsul Rizal Umami ◽  
Sarifa Siti Hapizah ◽  
Rosita Fitri ◽  
Aliefman Hakim
Keyword(s):  
Mus Musculus ◽  
Broiler Chicken ◽  
Egg Yolk ◽  
Extraction Methods ◽  
Colocasia Esculenta ◽  
Root Extract ◽  
Treatment Groups ◽  
Cholesterol Levels ◽  
Better Than

Abstrak : Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak umbi talas (Colocasia esculenta L) terhadap penurunan kadar kolesterol mencit putih (Mus musculus). Induksi hiperkolesterolemik dilakukan dengan pemberian kuning telur puyuh, pakan ayam broiler dan pemberian propiltiourasil (PTU) pada mencit putih (Mus musculus). Penelitian ini menggunakan metode ekstraksi dan uji penurunan kadar kolesterol dengan ekstrak umbi talas. Mencit dibagi menjadi 5 kelompok percobaan yang terdiri dari 3 kelompok perlakuan (P1, P2, dan P3) dan 2 kelompok kontrol (K+ dan K-). Dosis yang digunakan yaitu 100 mg/kgBB, dosis P2 yaitu 200 mg/kgBB, dan P3 yaitu 300 mg/kgBB. Sedangkan kelompok K+ diberikan dosis simvastatin dan K- hanya aquades. Hasil penelitian menunjukkan bahwa pada dosis 100 mg/kgBB terjadi peningkatan kadar kolesterol sebesar 5,2 mg/dL, pada K- juga mengalami peningkatan sebesar 3,8 mg/dL. Sedangkan pada P2 mengalami penurunan yang cukup tinggi sebesar 27,9 mg/dL, sementara P3 mengalami penurunan sebesar 2,4 mg/dL dan K+ juga mengalami penurunan sebesar 2,2 mg/dL. Berdasarkan hasil ini, dosis 200 mg/kgBB menunjukkan penurunan dosis yang lebih baik daripada dosis yang lain. Kata Kunci : Hiperkolesterolemik, PTU, Umbi Talas, Ekstraksi, Kolesterol Abstrack: This study aimed to determine the effect of taro root extract (Colocasia esculenta L) to decrease cholesterol levels of white mice (Mus musculus). Induction hiperkolesterolemik done by providing quail egg yolk, broiler chicken feed and administration of propylthiouracil (PTU) in white mice (Mus musculus). In this study using extraction methods and test cholesterol-lowering with taro root extract. The mice were divided into 5 experimental groups consisting of 3 treatment groups (P1, P2, and P3) and two control groups (K + and K-). In the group P1 doses used were 100 mg / kg body weight, P2 dose of 200 mg / kg, and P3 is 300 mg / kg. While the group K + and K- simvastatin dose given only distilled water. The results showed that at a dose of 100 mg / kg showed elevated levels of cholesterol by 5.2 mg / dL, the K- increased by 3.8 mg / dL. While at P2 decreased quite high by 27.9 mg / dL, P3 decreased by 2.4 mg / dL and K + also decreased by 2.2 mg / dL. Based on these results, a dose of 200 mg / kg showed a dose reduction is better than another dose. Keywords: Hiperkolesterolemik, PTU, Bulbs Talas, Extraction, Cholesterol

Experimental addition of Eleutherococcus senticosus and probiotic to the canine diet

2012 ◽  
Vol 7 (3) ◽  
pp. 436-447 ◽  
Author(s):  
Viola Strompfová ◽  
Iveta Plachá ◽  
Klaudia Čobanová ◽  
Soňa Gancarčíková ◽  
Dagmar Mudroňová ◽  
...  
Keyword(s):  
Current Trend ◽  
Antimicrobial Effect ◽  
Probiotic Strain ◽  
Serum Biochemistry ◽  
In Vitro Cultivation ◽  
Root Extract ◽  
Eleutherococcus Senticosus ◽  
Treatment Groups

AbstractThere is a current trend to support pet health through the addition of natural supplements to their diet, taking into account the high incidence of medical conditions related to their immune system and gastrointestinal tract. This study investigates effects of the plant Eleutherococcus senticosus as a dietary additive on faecal microbiota, faecal characteristics, blood serum biochemistry and selected parameters of cellular immunity in healthy dogs. A combination of the plant with the canine-derived probiotic strain Lactobacillus fermentum CCM 7421 was also evaluated. Thirty-two dogs were devided into 4 treatment groups; receiving no additive (control), dry root extract of E. senticosus (8 mg/kg of body weight), probiotic strain (108 CFU/mL, 0.1 mL/kg bw) and the combination of both additives. The trial lasted 49 days with 14 days supplementation period. Results confirm no antimicrobial effect of the plant on the probiotic abundance either in vitro (cultivation test) or in vivo. The numbers of clostridia, lactic acid bacteria and Gram-negative bacteria as well as the concentration of serum total protein, triglyceride, glucose and aspartate aminotransferase were significantly altered according to the treatment group. Leukocyte phagocytosis was significantly stimulated by the addition of probiotic while application of plant alone led to a significant decrease.

scholarly journals EVALUATION OF RENNELLIA ELLIPTICA AS POTENTIAL ANTIPLASMODIAL HERBAL REMEDY

2017 ◽  
Vol 79 (6) ◽  
Author(s):  
Che Puteh Osman ◽  
Nor Hadiani Ismail ◽  
Rohaya Ahmad ◽  
Aty Widyawaruyanti ◽  
Lidya Tumewu ◽  
...  
Keyword(s):  
Herbal Remedy ◽  
Extraction Methods ◽  
Significant Inhibition ◽  
Antiplasmodial Activity ◽  
Root Extract ◽  
Hplc Profile ◽  
Marker Compounds ◽  
Quantitative Analyses

Rennellia elliptica (Rubiaceae) has been used by local Jakun Community in the Endau Rompin State Park for the treatment of jaundice. Previous study has revealed the antiplasmodial activity of the root extract and major anthraquinones isolated from the roots. The present study entails the optimization of extraction methods, qualitative and quantitative analyses of selected marker anthraquinones and in vivo antiplasmodial activity along with toxicity and inhibition of β-hematin in vitro. HPLC profile showed the present of marker compounds as major constituents with content ranging 3-12 µg/g extract. The root extract showed potent antiplasmodial activity against rodent malaria, Plasmodium berghei with ED50 value of 1.23 µg/ml BW. The major anthraquinones, damnacanthal and nordamnacanthal showed significant inhibition against β-hematin formation via lipids and HRP2 catalyses. However, the root extract is slightly toxic against hepatocyte cell. These data suggests that R. elliptica is a potential herbal remedy for malaria treatment and antiplasmodial of the root extract possibly due to the action of major anthraquinones. 

scholarly journals Standardization of Diploid Codonopsis laceolata Root Extract as an Anti-Hyperuricemic Source

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2065
Author(s):  
Seung-Yub Song ◽  
So-Hyeon Bok ◽  
Sung-Ho Lee ◽  
Min-Hee Kim ◽  
Hee-Ock Boo ◽  
...  
Keyword(s):  
Quality Control ◽  
Xanthine Oxidase ◽  
Ethanolic Extract ◽  
Functional Materials ◽  
Extraction Methods ◽  
Oxidase Inhibitor ◽  
Root Extract ◽  
Xanthine Oxidase Inhibitor

Codonopsis lanceolate exerts various medicinal effects and has been used as a traditional medicine for inflammation, asthma, gastritis, and liver disease. Recently, we reported the xanthine oxidase inhibitory activity of C. lanceolata extract and that lobetyolin, one of the key components, was a xanthine oxidase inhibitor. Lobetyolin showed anti-hyperuricemic activity in vitro and in vivo. In this study, we prepared various types of C. lanceolata extracts for the development of functional materials and natural drugs. We present the optimal analytical approach for the quality control and extraction optimization of C. lanceolata preparations. We established and validated a HPLC analysis for easy separation and quantification of the lobetyolin biomarker. Solvent extracts of C. lanceolata root were prepared and the profiles of the active marker and the optimal extraction methods were evaluated. The 100% ethanolic extract demonstrated the highest lobetyolin content. The validated HPLC method confirmed that lobetyolin was present in C. lanceolata root extracts. We suggest that the anti-hyperuricemic activities of C. lanceolata extract could be attributed to this marker compound. The results proposed that the 100% ethanolic extract could be used for the prevention of hyperurecemia, and that this analytical method and biomarker could be useful for the quality control of C. lanceolata preparations.

Efficacy of diatomacious earth in amelioration of aflatoxininduced toxicity in broiler chicken

2016 ◽  
Author(s):  
A. W. Lakkawar ◽  
M. L. Sathyanarayana ◽  
H. D. Narayanaswamy ◽  
SPACE Sugunarao ◽  
S. Yathiraj ◽  
...  
Keyword(s):  
Broiler Chicken ◽  
Growth Parameters ◽  
Body Weight Gain ◽  
Serum Levels ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Negative Effects ◽  
Total Proteins ◽  
Treatment Groups ◽  
Cholesterol Levels

The ability of Diatomacious earth (DAE) in reducing the toxic effects of aflatoxin (AF) in broiler diet was evaluated. DAE was supplemented @ 400 and 800 mg kg-1 of feed along with 0.5 and 1 ppm of AF kg-1 of feed. Healthy unsexed day old broiler chicks (n=360) were assigned to 9 groups comprising of control and treatment groups. Feeding of AF resulted in significantly lower feed intake as well as body weight gain and increase in feed conversion ratio in comparison to the control groups. Supplementation of DAE in aflatoxin mixed diet significantly reduced the deleterious effects of AF on growth parameters. Feeding of AF alone caused significant decrease in serum levels of total proteins, albumin, triglycerides and cholesterol. However, significant increase was found in serum levels of AST, ALT, ALP, BUN and creatinine in AF fed broilers. Supplementation of DAE to the AF mixed feed ameliorated the adverse effect of AF on the serum biochemical values by causing increase in serum total proteins, albumin, triglycerides and cholesterol levels. In addition, a significant increase was recorded in AST, ALT, ALP, BUN and creatinine levels in DAE supplemented broilers. The study concluded that diatomaceous earth is an effective adsorbent to decrease the negative effects of aflatoxin in broiler chicken.

scholarly journals Increasing Atherosclerosis in Streptozotocin-Induced Diabetes into Four Groups of Mice

2018 ◽  
Vol 6 (2) ◽  
pp. 287-292 ◽  
Author(s):  
Yunita Sari Pane ◽  
Aznan Lelo ◽  
Kiking Ritarwan ◽  
Nerdy Nerdy
Keyword(s):  
Blood Glucose ◽  
Protective Effect ◽  
Egg Yolk ◽  
Heart Tissue ◽  
Diabetic Mice ◽  
Treatment Groups ◽  
Cholesterol Levels ◽  
Streptozotocin Induced Diabetes ◽  
Genetically Diabetic Mice ◽  
All Treatment

AIM: To study the protective effect of medicines on the formation of atherosclerosis in mice, it is needed to conduct the study in mice which is not genetically diabetic mice induced by streptozotocin (STZ) to produce hyperglycemia and atherosclerosis, compared with mice treated by yolk or its combination.MATERIAL AND METHODS: Fifty-six mice, Double Deutch Webster strain, male, receive  10 weeks, 20 - 30 gr bodyweight were divided into 4 groups (n = 14) i.e. control (do not received any agents), STZ (45 mg/kg/BW was injected intraperitoneally for 5 days), yolk (0.2 cc orally daily for 6 weeks), and combination of STZ and yolk (STZ: 45 mg/kg/BW intraperitoneally add 0.2 cc yolk orally). All animals were executed in the 42nd day. Then, the aorta of the mice’s heart tissue was histopathology examined. Blood glucose and cholesterol levels were determined every week.RESULTS: Hyperglycemia occurred in mice induced by STZ injection with the highest BGL (521.8 ± 48.2 mg/dl; 188.4%) in the 4th-week observation; after that BGL decrease. We found that, except the control, all treatment groups with STZ, egg yolk, and combination underwent atherosclerosis.CONCLUSION: The present study was able to demonstrate the occurrence of atherosclerosis in mice treated by STZ accompanied with increasing blood glucose and cholesterol level.

scholarly journals Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam

2018 ◽  
Vol 2 (2) ◽  
pp. 119-125
Author(s):  
Esty Tyastirin ◽  
Risa Purnamasari ◽  
Irul Hidayati ◽  
Eva Agustina ◽  
Moch. Irfan Hadi ◽  
...  
Keyword(s):  
Mus Musculus ◽  
Laboratory Experiments ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Sucrose Induction ◽  
Cholesterol Levels ◽  
Randomized Design ◽  
Gaussian Data

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.

scholarly journals Respon Histopatologis Hepar Mencit (Mus musculus) yang Diinduksi Benzo(α)Piren terhadap Pemberian Taurin dan Ekstrak Daun Sirsak (Annona muricata)

2017 ◽  
Vol 16 (2) ◽  
pp. 54
Author(s):  
Annisa Agata ◽  
Endang Linirin Widiastuti ◽  
G. Nugroho Susanto ◽  
Sutyarso '
Keyword(s):  
Mus Musculus ◽  
Leaf Extract ◽  
Corn Oil ◽  
Annona Muricata ◽  
Group V ◽  
Group Iii ◽  
Treatment Groups ◽  
Randomized Design ◽  
Group I

Cancer is a disease that is characterized by the existence of damage and cell abnormality in growth and differentiation. Liver cancer is a disorder of hepar tissue derivated from its tumors. Taurine is known as antioxidant but its role as anticancer needs to be explored more as well the role of Annona muricata leaf extract which is believed to have its role as anticancer substance. This research, therefore, aimed to explore the effect of taurine and Annona muricata leaf extract on the hepar histopathology of male mice (Mus musculus) induced by benzo(α)pyren in vivo. This research was carried out by using a complete randomized design, which consisted of 5 treatment groups which was repeated 5 times. Group I was given 0.2 mL corn oil for 15 days, group II was induced by benzo(α)pyren without taurin nor A. Muricata leaf extract for 10 days, group III was given 7.8 mg taurine/BW/day (twice a day) starting from the 15 th days before the induction of benzo(α)pyren, group IV, after induced with benzo(α)pyren, taurine was given with dosage of 7.8 mg/BW/day, group V, after induced with benzo(α)pyren, soursop leaf extract was given with amount of 277.8 mg/BW/day. Data analyzed by Kruskal-Wallis test and one way ANOVA with Fisher test (p>0.05). The results indicated that taurine had ability to recover the liver tissue induced by benzo(α)pyren as (carcinogenic) while, Annona muricata leaf extract had not shown any recover of tissue damage

scholarly journals Potensi Ekstrak Akar Kalakai (Stenochlaena palutris Bedd) Sebagai Antihiperlipidemia Yang Diuji Secara In Vivo

2020 ◽  
Vol 7 (1) ◽  
pp. 62
Author(s):  
Rabiatul Adawiyah ◽  
Fera Sartika ◽  
Fahruddin Arfianto
Keyword(s):  
Medicinal Plants ◽  
Cell Surface ◽  
Total Cholesterol ◽  
Wistar Rats ◽  
Root Extract ◽  
Cholesterol Levels ◽  
Antihyperlipidemic Activity ◽  
Alternative Drug ◽  
Increased Activity

ABSTRAK Tumbuhan kalakai atau sering disebut paku haruan atau pakis  merupakan tumbuhan khas Kalimanatan yang banyak digunakan sebagai tumbuhan obat. Kalakai mengandung beberapa senyawa bioaktif seperti fenolik, flavonoid, alkaloid dan keluarga terpenoid yang efektif sebagai antioksidan.  Tujuan penelitian ini untuk menentukan aktivitas antihiperlipidemia dar i ekstrak akar kalakai yang diuji secara in vivo pada tikus wistar. Metode yang digunakan adalah melakukan uji aktivitas antihiperlipidemia dengan cara memberi perlakuan hewan coba dan melakukan pengukuran kadar trigliserida dan kolesterol total dengan menggunakan Spektrofotometer UV-VIS. Hasil penelitian diketahui bahwa peningkatan dosis dapat meningkatkan aktivitas dari ekstrak akar  kalakai. Semakin meningkatnya dosis,  maka terjadi peningkatan pertemuan antara flavonoid dengan reseptor di permukaan sel, sehingga terjadi peningkatan aktivitas. Aktivitas dari pemberian dosis ke-3 dari kadar trigliserida sebesar 49,8 mg/dl sedangkan untuk kadar kolesterolnya sebesar 41,8 mg/dl pada hasil yang didapat dari ekstrak akar kalakai  mendekati pemberian dari kadar  simvastatin. Berdasarkan hal tersebut, maka ekstrak dengan dosis 400 mg/KgBB memiliki potensi untuk teliti lebih lanjut dan dapat digunakansebagai obat alternatif pada pengobatan hiperlipidemia. Keadaan hiperlipidemia tidak hanya terkait kolesterol, tetapi mencakup kadar trigliserida di dalam darah. Kemampuan suatu obat dalam menurunkan kadar kolesterol sekaligus kadar trigliserida, akan memberikan keuntungan terhadap pengobatan pasien hiperlipidemia. Penggunaan akar kalakai tidak hanya terbatas pada pengobatan karena bahan alam dapat digunakan sebagai pencegah timbulnya penyakit terutama hiperlipidemia yang terjadi pada pasien degeneratif. Kata kunci : Ektrak akar kalakai, In Vivo, Kolesterol, SpektrofotometerUV-VIS, Trigliserida  ABSTRACT Kalakai plants or often called haruan nails or ferns are typical Kalimanatan plants which are widely used as medicinal plants. Kalakai contains several bioactive compounds such as phenolic, flavonoid, alkaloid and terpenoid family which are effective as antioxidants. The purpose of this study was to determine the antihyperlipidemic activity of kalakai root extract which was tested in vivo in Wistar rats. The method used is to test the activity of antihyperlipidemia by giving treatment of experimental animal and to measure levels of triglycerides and total cholesterol using a UV-VIS spectrophotometer. the results of the study note that increasing the dose can increase the activity of the kalakai root extract. As the dose incr eases, there is an increase in the linkage between the flavonoids and receptors on the cell surface, resulting in increased activity . The activity of administering the 3rd dose of triglyceride levels was 49.8 mg/dl whereas for cholesterol levels it was 41.8 mg/dl in the result obtained from the extract of kalakai root approaching the administration of simvastatin levels. Based on this, the extract at a dose of 400mg/kg has the potensial for further scrutiny and can be used as an alternative drug in the treatment of hyperlipidemia. The state of hyperlipidemia is not only related to cholesterol, but includes the level of triglycerides in the blood. The ability of a drug to reduce cholesterol levels as well as triglyceride levels, will provide benefits to the treatm ent of hyperlipidemia patients. The use of kalakai root is not only limited to treatment because natural ingredients can be use d as a prevention of disease, especially hyperlipidemia that occurs in degenerative patients. Keywords: Kalakai root extract, In Vivo, Cholesterol, Triglyceride, UV-VIS Spectrophotometer

Effect of mannan-oligosaccharides (MOS) supplementation on performance, immunity and HSP70 gene expression in broiler chicken during hot-dry summer

2017 ◽  
Author(s):  
J. J. Rokade ◽  
Mukesh Kagate ◽  
S. K. Bhanja ◽  
M. Mehra ◽  
A. Goel ◽  
...  
Keyword(s):  
Broiler Chicken ◽  
Control Diet ◽  
Dietary Treatment ◽  
Hsp70 Expression ◽  
Broiler Chicks ◽  
Standard Diet ◽  
Treatment Groups ◽  
Cholesterol Levels ◽  
Production Indices ◽  
Cellular And Humoral Immunity

An experiment was conducted on broiler chicks (n=120), reared in cages on a standard diet up to 14th day of age and thereafter up to 42nd day (14-42d) on test diets with or without MOS. The 14th day old chicks were randomly distributed into three dietary treatment groups viz., T1 (control diet), T2 (control diet with MOS @ 0.3%) and T3 (control diet with MOS @ 0.5%). Each dietary treatment comprised of four replicates of eight birds each. Experiment was carried out during hot-dry summer. It was found that production indices improved (P>0.001) significantly during all phases. Cellular and humoral immunity at 28th day of age improved significantly (P>0.05) due to MOS supplementation. The percentage of hemoglobin, protein, AST and ALT increased significantly (P>0.001) in MOS supplemented group at 28th as well as 42nd day of age. While H:L ratio, serum corticosterone and cholesterol levels decreased significantly (P>0.001) in MOS supplemented groups. Supplementation of MOS at the both levels caused significant (P>0.001) down regulation of HSP70 expression in jejunum tissues during 28th and 42nd day. Based on this study it was concluded that supplementation of 0.3% MOS in broiler diets significantly improved their performance and welfare during heat stressed conditions.

scholarly journals Rasyona Kakule Tozu İlavesinin Yumurtlayan Bıldırcınlarda Performans, Yumurta Kalitesi ve Serum Biyokimyasal Parametrelerine Etkisi

2021 ◽  
Vol 9 (11) ◽  
pp. 1999-2003
Author(s):  
Osman Olgun ◽  
Esra Tuğçe Gül ◽  
Alpönder Yıldız ◽  
Abdullah Çolak
Keyword(s):  
Serum Cholesterol ◽  
Feed Intake ◽  
Egg Quality ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Treatment Groups ◽  
Cholesterol Levels ◽  
A Value ◽  
L Value ◽  
Different Levels

The present research was carried out to determine the effects of the addition of cardamom powder at different levels (0, 1, 2, 3 or 4 g/kg) to diet on performance, egg quality, and serum parameters in laying quails. In the experiment, a total of 120 laying quails at the age of 20 weeks were randomly distributed to 5 treatment groups with 6 subgroups (4 quails each). Quails were fed with experimental diets for 10 weeks. In the experiment, the feed intake of quails significantly increased with the supplementation of cardamom powder at the level of 3 g/kg to the diet, while other performance parameters were not affected. Eggshell thickness advanced with the addition of 1 g/kg cardamom powder to diet. Egg yolk L* value increased at the level of 4 g/kg, but a* value decreased with the addition of cardamom powder. Other egg quality parameters were not affected by the addition of cardamom powder to the diet. In addition, the supplementation of cardamom powder to the diet significantly reduced serum cholesterol levels of quails. According to the results obtained from the experiment, the addition of cardamom powder at the level of 3 g/kg to the diet increased the feed intake of quails, while the supplementation of 1 g/kg was effective in improving the eggshell quality and serum cholesterol level.

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