scholarly journals Histology of hematoxylin–eosin and immunohistochemical diabetes rat pancreas after giving combination of moringa leaves (Moringa oleifera) and clove flower (Syzygium aromaticum) extracts

2021 ◽  
Vol 9 (A) ◽  
pp. 257-262
Author(s):  
Purnama Ningsih ◽  
Sitti Rahmawati ◽  
Baharuddin Hamzah ◽  
Tri Santoso ◽  
Nurbaya Nurbaya ◽  
...  
Keyword(s):  
Body Weight ◽  
Beta Cells ◽  
Positive Reaction ◽  
Test Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Combination Group ◽  
Control Group ◽  
Single Test

The purpose of this study was to determine the regeneration activity of pancreatic beta cells by a combination of the ethanol extracts of Moringa leaves and Clove flower. These extracts prepared by maceration with ethanol as solvent . This is an experimental laboratory research by using post test only group design . The subject of this research is 40 white male rats of Wistar strain ( Rattus norvegicus ) and were conditioned DM by streptozotozine-nicotinamide induction . The rats randomly divided into 8 groups , and each group consisted of 5 rats . Combination dose of Moringa leaves and Clove flowers extracts used are  150 : 40 mg / kg body weight of mice , 100 : 80 mg / kg body weight of mice and 50 : 120 mg / kg body weight of mice . The rats were sacrificed and the pancreas taken to be made preparations histopathology , staining and observation tissues with HE and IHC methods. The results showed that the staining and observation tissues of heart with  HE method acquired the positive control group , the single test group and the combination group  were showing the islet on the Langerhans island of the pancreas is bigger than in the negative control group. The morphology of the island of Langerhans increasingly large indicates increasing the regeneration of the cells of the islet beta pancreas; whereas the method of IHC acquired the negative control group seen lack of display intensity of positive reaction Ag  & Ab beta cells with are marked with brown cells . In the positive control group , the single test group and the combination group , seen a positive reaction of Ag & Ab insulin-producing beta cells characterized by brown cells and insulin expression dominating pancreatic island of Langerhans.

scholarly journals BLACK BANGLE (ZINGIBER OTTENSII VAL.) RHIZOME AND KATUK LEAVES (SAUROPUS ANDROGYNUS L. MERR) EXTRACT COMBINATION PROTECTIVE ROLE ON ADIPOSE TISSUES HISTOLOGIC PROFILE OF HIGH-FAT AND CARBOHYDRATE DIET-INDUCED OBESE MALE RATS

2018 ◽  
Vol 11 (13) ◽  
pp. 225
Author(s):  
Sulaeman A ◽  
Patonah Patonah ◽  
Patonah Patonah ◽  
Negara Gg ◽  
Negara Gg
Keyword(s):  
Test Group ◽  
Negative Control Group ◽  
Protective Role ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
High Fat ◽  
Adipose Tissues ◽  
Obese Male

  Objective: The effect of Zingiber ottensii Val. rhizome and Sauropus androgynus L. Merr leaves extract combination was investigated using histologic profile of adipose tissues in obese male rats induced by high-fat and carbohydrate diets.Methods: This was a preventive study, conducted for 42 days by simultaneous administration of diets and extracts administration. The subjects were divided into 8 groups. All groups except negative control group were fed with high-fat and carbohydrate diets. Orlistat, metformin, and curcumin were used as contrast.Result: The phytochemical screening of Z. ottensii Val. rhizome extract showed the presence of flavonoids, saponins, and triterpenoids, meanwhile S. androgynus L. Merr leaves extract presented flavonoids, tannins, saponins, steroids, and triterpenoids. The results showed tissues histological differences in all test group compared with positive control. The most effective combination dose for bangle and katuk leaves extract in protecting adipose tissue was 100 mg/Kg:100 mg/Kg body weight.Conclusion: The combination of black bangle and katuk leaves extract showed a protective role, demonstrated by adipose tissues histologic profile.

scholarly journals Ameliorative Effect of Ethanolic Extract of Petroselinum Crispum Against Gentamicin-Induced Nephrotoxicity in Rabbits Male

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Sarah Hussain ◽  
Mariam Kadhem
Keyword(s):  
Body Weight ◽  
Clinical Signs ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Positive Control Group ◽  
Control Group ◽  
Petroselinum Crispum

The experiment was conducted to investigate the protective effect of Petroselinum crispum leave extracted against gentamicin-induced nephrotoxicity in male rabbits by studying the body weight, clinical signs, haematological and biochemical parameters, gross lesion and histopathological changes. Twenty four rabbits male were used and divided into 4 groups. Group 1: rabbits served as a negative control, received distilled water 1 ml(orally). Group 2: rabbits served as a positive control group, received gentamicin at a dose of 80 mg/kg/day intramuscular for 15 days. Group 3: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 125 mg/kg orally for 15 days. Group 4: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg orally for 15 days.The results of the gentamicin treated group( positive control group) showed clinical signs such as loss of body weight, loss of appetite and rough hair with hematuria. The body weight a significantly declined (p≤ 0.05) compared other groups. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, GSH, SOD, CAT, and GPX levels, while it recorded a significant increase (p≤0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA. Histological studies showed several kidney pathological changes such as pale colour, enlargement in size and weight and easy from detaching as opposed to negative control group. On the other hand, the group treated with ethanolic extractof Petroselinum crispum at dose 125 mg/kg induced improved of parameters as recorded significant increased(P ≤ 0.05) in body weight, WBC count, lymphocyte, GSH, SOD, CAT, and GPX, while significant decreased (P ≤ 0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA compared with the positive control group whereas rabbits treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg restored the parameters and histological changes of the kidney to near normal status compared with the negative control group. These results showed that the dose-detected Petroselinum crispum extract (250mg / kg) acts as potential curative effect against gentamicin-induced nephrotoxicity in male rabbits.

scholarly journals Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2

2021 ◽  
Vol 4 (2) ◽  
pp. 73
Author(s):  
Putri Ayu Ika Setiyowati ◽  
Rofiatun Solekha ◽  
Sri Bintang Sahara Mahaputra Kusuma Negara ◽  
Reny Rosalina
Keyword(s):  
Body Weight ◽  
Optimal Dose ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Optimal Dosage ◽  
Skin Injury ◽  
Treatment Groups

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used.  Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of  0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups  were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw.  In day 21 all group were injected with 0,2 ml of  pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.

Hepatoprotective Role of AV/LCP/18 In CCl4 Induced FLKS Syndrome in Commercial Broilerst

2016 ◽  
Vol 12 (2) ◽  
Author(s):  
P. M. Sonkusale ◽  
Pratik N. Inglepatil ◽  
Siddharth V. Jamadade
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Protein Profile ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Mean Values ◽  
Group B

A total of 75 day old broiler chicks were divided into three equal groups. Group A served as negative control. Group B (positive control) and C (treatment group) were intoxicated with oral administration of CCl4 @ 1 ml/kg body weight after every 3rd day during 15-28 days of age. Group C was given AV/LCP/18 @ 0.10 ml / chick / day in drinking water during 29th-42nd day of age. At the end of experiment, significantly (P is less than 0.01) higher mean body weight and lower FCR was observed in treatment group C as compared to positive control group B. Similarly, significantly (P is less than 0.05) decreased levels of SGOT, SGPT, cholesterol, triglycerides, creatinine and higher mean values of serum protein profile along with mild degenerative changes in liver and kidney of broilers in group C were observed as compared to group B indicating therapeutic efficacy of AV/LCP/18 in ameliorating the toxic effects or FLKS induced by CCl4 in commercial broilers

scholarly journals Antipyretic effect of key lime (Citrus aurantiifolium) leaf extract on white rats

2012 ◽  
Vol 10 (2) ◽  
pp. 35-39
Author(s):  
AMELIA KARTIKA WIDOWATI ◽  
NUR HAFIDHA HIKMAYANI ◽  
ETI PONCORINI PAMUNGKASARI
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antipyretic Effect ◽  
White Rats ◽  
Key Lime ◽  
One Way Anova

Widowati AK, Hikmayani NH, Pamungkasari EP. 2012. Antipyretic effect of key lime (Citrus aurantifolia) leaf extract on white rats. Biofarmasi 10: 35-39. The objective of this study was to evaluate the antipyretic effect of key lime (Citrus aurantifolia L.) leaf extract on white rats (Rattus norvegicus) induced fever by using DPT vaccine. Thirty male white rats with the body weight of ±200 grams and the age of 2-3 months old were used in this study. Those rats were grouped equally into five groups, consisted of a negative control group (2 mL aquabidest), a positive control group (acetaminophen 6.3 mg/100 g of body weight), the first dose of key lime leaf extract (1.26 mg/100 g of body weight), the second dose (2.52 mg/100 g of body weight) and the third dose (5.04 mg/100 g of body weight). The measurement of rat temperature was conducted before and 2 hours after getting DPT vaccine, and every 30 minutes posttreatment until 120 minutes. Data at the 120th minute were analyzed by using One-Way Anova test. The results of One-Way Anova test showed that there were significant differences (p<0.05) among treatment groups. The results of post-hoc test analysis showed that the significant differences (p<0.05) were found between a negative control group and four other groups. There was no significant difference (p>0.05) between a positive control group and three groups of key lime leaf extract. It concluded that the key lime leaf extract has an antipyretic effect on white male rats.

Oral Rehydration Salt-Liquid as an Alternative Storage Medium - A Preliminary Study

2011 ◽  
Vol 35 (4) ◽  
pp. 393-396 ◽  
Author(s):  
P Subramaniam ◽  
U Eswara ◽  
KL Girish Babu ◽  
B Vardhan
Keyword(s):  
Test Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Oral Rehydration Salt ◽  
Control Group ◽  
Oral Rehydration ◽  
Pdl Cells ◽  
The Mean ◽  
Group 5

Objective: To assess the efficacy of Oral Rehydration Salt-Liquid (ORS-L) in the maintenance of the viability of Periodontal ligament cells (PDL). Materials and Method: Twenty freshly extracted teeth were used for this study. They were then randomly divided into 3 groups: Positive control group - 5 teeth which were immediately subjected to collagenase assay, without immersing in ORS-L; Negative control group - 5 teeth with an extra oral dry time of 24 hours, followed by subjecting to collagense assay without immersing in ORS-L and Test group (ORS-L) - 10 teeth with an extra oral dry period of 30 minutes, followed by immersion in ORS-L for a period of 45 minutes and then subjected to collagenase assay. The mean number of viable PDL cells were counted on a hemocytometer under 20X magnification. Results: The mean number of viable PDL cells was highest in the positive control group. In comparison to the negative control group, the test group showed a higher number of viable PDL cells. Conclusion: The study found that ORS-L was an effective solution in maintaining the viability of PDL cells.

scholarly journals Effect of dietary supplementation of ginger extract on growth, carcass characteristics and haematological parameters in broilers

2017 ◽  
Vol 3 (2) ◽  
pp. 211-215 ◽  
Author(s):  
Sudipta Talukder ◽  
Md Mehedi Hasan ◽  
Zakaria Al Noman ◽  
Yousuf Ali Sarker ◽  
Torun Kumar Paul ◽  
...  
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Ginger Extract ◽  
Final Body Weight

This study evaluated the effect of ginger on the growth performance, carcass quality of broiler chickens. 20 broiler chickens were randomly selected into two treatment groups identified as T1 and T2 with a positive control and a negative control group. Each treatment contained with five birds. Birds on T1 were treated with 1% ginger extract and T2 was treated with 2% ginger extract via drinking water. Significant variations (p<0.05) existed between the control and other treatments in mean final body weight, dressed weight, daily feed intake and feed conversion ratio. At the end of experiment (35th day) for 1% ginger extraction treatment the live body weight is 1746gm (p<0.05), dressing weight 1106.4 (p<0.05) and FCR is 1.67(p<0.05). The usage of the test ingredients had a significant effect (p<0.05) on dressing percentage. Supplementation of ginger improves the performance of broilers when added at the rate of 1% of broiler ration and can be a possible alternative to antibiotic growth promoter in the feeding of broiler chicken.Asian J. Med. Biol. Res. June 2017, 3(2): 211-215

scholarly journals The Antidiabetic Effect of Low Doses ofMoringa oleiferaLam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Abdulrahman L. Al-Malki ◽  
Haddad A. El Rabey
Keyword(s):  
Body Weight ◽  
Diabetic Nephropathy ◽  
Glycosylated Hemoglobin ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Tissue Homogenate ◽  
Control Group ◽  
Low Doses

The antidiabetic activity of two low doses ofMoringaseed powder (50 and 100 mg/kg body weight, in the diet) on streptozotocin (STZ) induced diabetes male rats was investigated. Forty rats were divided into four groups. The diabetic positive control (STZ treated) group showed increased lipid peroxide, increased IL-6, and decreased antioxidant enzyme in the serum and kidney tissue homogenate compared with that of the negative control group. Immunoglobulins (IgA, IgG), fasting blood sugar, and glycosylated hemoglobin(HbA1c)were also increased as a result of diabetes in G2 rats. Moreover albumin was decreased, and liver enzymes andα-amylase were not affected. In addition, the renal functions and potassium and sodium levels in G2 were increased as a sign of diabetic nephropathy. Urine analysis showed also glucosuria and increased potassium, sodium, creatinine, uric acid, and albumin levels. Kidney and pancreas tissues showed also pathological alteration compared to the negative control group. Treating the diabetic rats with 50 or 100 mgMoringaseeds powder/kg body weight in G3 and G4, respectively, ameliorated the levels of all these parameters approaching the negative control values and restored the normal histology of both kidney and pancreas compared with that of the diabetic positive control group.

scholarly journals Evaluation of Antimalarial Properties of Ficus platyphylla Del Leaf Extract in Mice

2019 ◽  
pp. 1-8
Author(s):  
J. Omame ◽  
A. E. Amobonye ◽  
A. Y. Kabiru
Keyword(s):  
Body Weight ◽  
Plant Extract ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Crude Plant Extract ◽  
Different Doses

Aim: This study was aim at investigating the effect of crude petroleum ether leaf extract of Ficus platyphylla Del on Plasmodium berghei infected mice. Place and Duration of Study: This research was carried out at the department of biochemistry, Federal university of technology minna, Niger state Nigeria in 2014. Methodology: The crude plant extract of F. platyphylla was administered 72 hours at different doses post and pre infection for both the curative and prophylactic study respectively against residual infection. Mice were divided into 5 groups of 5 mice each, 3 of the groups where administered crude plants extract of F. platyphaylla at different doses (200, 400 and 600 mg/kg body weight) while the other two serve as negative and positive control group and were administered 0.5 ml and 50 mg/kg body weight respectively. Results: The extract at all doses produced significant (P<0.05) dose dependent chemo-suppressive activity with %  inhibition of 38%, 61%, 74% and 81.8% for curative studies and 36.0%, 38.5%, 49.5% and 63.4% for prophylactic studies against the parasites at doses of 200 mg/kgbw, 400 mg/kgbw, 600 mg/kgbw of the extract and 50 mg/kgbw of Artesunate. All doses of the extract increased the survival time of the infected mice compared to the negative control group that was administered 0.5 ml normal saline. The variation in the values of Packed Cell Volume (PCV) for treated group before and after extract administration was not significant at (P<0.05). The phytochemical screening of the plant extract showed the presence of tannin, saponin, flavonoids, terpenoids, steroids, anthroquinone and phenol. Conclusion: The result of this study shows that F. Platyphylla leaf extract exhibited some antiplasmodial activity that could be exploited for safe, effective and affordable antimalaria regimen.

scholarly journals Promotor effect of S-metolachlor generics with different hepatotoxicity in liver carcinogenesis in rats

2019 ◽  
Vol 86 (2) ◽  
pp. 5-13
Author(s):  
E.A. Bagley ◽  
N.M. Nedopytanska ◽  
V.S. Lisovska ◽  
O.V. Reshavska ◽  
L.V. Tkachenko
Keyword(s):  
Body Weight ◽  
Liver Cancer ◽  
Constitutive Androstane Receptor ◽  
Clinical Signs ◽  
Negative Control Group ◽  
Epidemiological Studies ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Species Specific

Metolachlor and currently its biological active isomer S–metolachlor is one of the most widely used herbicides in the world. Chronic experiments in rats have found hepatocarcinogenic effect of metolachlor, and epidemiological studies have found positive relationship between enzyme exposure to metolachlor and prevalence of liver cancer. Possibility of the influence of harmful impurities contained in technical products on the detected effects is emphasized. Objective is to study promotor effect of S–metolachlor generics with different hepatotoxicity in carcinogenesis of liver in rats induced by nitrosodiethylamine (NDEA) and analyse possibility of its realisation in human. Materials and Methods. Experiments were performed in male Wistar Han rats on hepatocarcinogenesis model “NDEA — hepatectomy”. Two specimens of S–metolachlor generics were studied; and their ratio of S/R enantiomers was 87/13 % with different hepatotoxicity. Substances were administered intragastrically in the doses of 1.5,15 and 150 mg/kg body weight for 8 weeks. Animals of the negative control group received water, and positive control — phenobarbital. Promotor effect was evaluated by the standardised parameters of the total area and number of hepatocyte foci expressing γ-glutamyl transpeptidase (GTP). Results. No clinical signs of the toxic action of S–metolachlor on the rat body induced to carcinogenesis by NDEA were found. Increase in the number and total area of γ-GTP positive foci in the liver of animals on tumorogenic dose of both specimens of S–metolachlor as well as phenobarbital was found. Mean area of focus in the liver of rats on more toxic specimen was lower. The threshold of promotor action of S–metolachlor on hepatocarcinogenesis has been established at the level of γ 15 mg/kg body weight. Analysis of literature data on the mechanism of hepatotoxic action of metolachlor allowed to make a conclusion aboutphenobarbital-like mechanism of promotor action that is realised through constitutive androstane receptor (CAR). This mechanism is species-specific for rodents; therefore, the results of epidemiological studies on the possibility of liver cancer in human cannot be confirmed experimentally. Conclusion. Tumorogenic dose of S–metolachlor generics with different degree of hepatotoxicity shows promotor effect in NDEA induced carcinogenesis in rat liver. Hepatotoxicity of S–metolachlor inhibits growth of γ-GTP positive foci. The threshold of hepatocarcinogenesis promotion has been established at the level of γ 15 mg/kg body weight. The mechanism of the observed effect is not relevant for human. Key Words: S–metolachlor, hepatocarcinogenesis initiated by nitrosodiethylamine, Wistar Han rats, γ-glutamyltranspeptidase.

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