Simultaneous Determination of 10-Deacetylbaccatin，Cephalomannine and Taxol in Different Parts of Taxus Media with Different Growth Years by UPLC

2011 ◽  
Vol 108 ◽  
pp. 326-330 ◽  
Author(s):  
Jing Zhang ◽  
Chao Feng Yang ◽  
Ke Yuan
Keyword(s):  
Ultra Performance Liquid Chromatography ◽  
Scientific Basis ◽  
Sichuan Province ◽  
Zhejiang Province ◽  
Taxus Baccata ◽  
Active Components ◽  
Taxus Media ◽  
Hybrid Variety ◽  
Taxus Cuspidate ◽  
Different Parts

Taxus media is the natural hybrid variety of Taxus cuspidate and Taxus baccata, and has been used as an anticancer plant with some medical components-taxanes. In this study, ultra performance liquid chromatography was used for quantification of three major bioactive taxanes in Taxus media, which were collected in Zhejiang province and Sichuan province (China). As a result of this study, a convenient, accurate, rapid and reproducible UPLC method has been developed to successfully quantify 10-Deacetylbaccatin Ⅲ (10-DABⅢ), Cephalomannine and Taxol in the needle and twig of Taxus media. The results indicate that the sequence of the contents of three active components in Taxus media with different parts was needle>twig; the sequence of the contents of three active components in Taxus media with different growth years was 5 year old >4 year old >3 year old; and there were great differences in the contents of three active components in Taxus media between Zhejiang province and Sichuan province. The results also provided a scientific basis for uses of Taxus media.

Uşak İli’nden Toplanan Taxus Baccata (L) Türünün Metanol Ekstresinin Yaprak, Dal ve Meyvesinde Biyolojik Aktivitenin Belirlenmesi

2019 ◽  
Vol 7 (10) ◽  
pp. 1533
Author(s):  
Yasemin Karafakıoglu
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Maximum Amount ◽  
Chemical Components ◽  
Taxus Baccata ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Future Studies ◽  
Different Parts

Taxus species in different parts of the world prevent the formation of hepatoprotective, anticoagulant, antiulcerogenic, anti-coagulant, antifungal and tumour cells. In this study, biological activity studies were performed on the leaves, branches and fruits of the methanol extract of Taxus baccata L. species collected from Sivaslı district of Uşak province. The methanol extract of the T.baccata species, the total amount of phenolic content of the sample was found to be 14.76 mg GAEQ/1g dry sample in the highest leaves. In T.baccata methanol extract, the maximum amount of flavonoid content was 0.468±mg QE/g dry sample. Antioxidant activity value as DPPH radical; % inhibition value was found as 93.21%. Based on the results; It can be concluded that the leaves of T.baccata have higher antioxidant activity than fruits and branches. Future studies need to explore the chemical components contained in the T.baccata species to determine and investigate in further detail.

scholarly journals Mechanism of Compound Houttuynia Mixture as an Anti-COVID-19 Drug Based on Network Pharmacology and Molecular Docking

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110167
Author(s):  
Xing-Pan Wu ◽  
Tian-Shun Wang ◽  
Zi-Xin Yuan ◽  
Yan-Fang Yang ◽  
He-Zhen Wu
Keyword(s):  
Molecular Docking ◽  
Target Prediction ◽  
Interaction Network ◽  
Scientific Basis ◽  
Network Pharmacology ◽  
Chemical Components ◽  
Active Components ◽  
Discovery Studio ◽  
The Core ◽  
Pathway Annotation

Objective To explore the anti-COVID-19 active components and mechanism of Compound Houttuynia mixture by using network pharmacology and molecular docking. Methods First, the main chemical components of Compound Houttuynia mixture were obtained by using the TCMSP database and referring to relevant chemical composition literature. The components were screened for OB ≥30% and DL ≥0.18 as the threshold values. Then Swiss Target Prediction database was used to predict the target of the active components and map the targets of COVID-19 obtained through GeneCards database to obtain the gene pool of the potential target of COVID-19 resistance of the active components of Compound Houttuynia mixture. Next, DAVID database was used for GO enrichment and KEGG pathway annotation of targets function. Cytoscape 3.8.0 software was used to construct a “components-targets-pathways” network. Then String database was used to construct a “protein-protein interaction” network. Finally, the core targets, SARS-COV-2 3 Cl, ACE2 and the core active components of Compound Houttuyna Mixture were imported into the Discovery Studio 2016 Client database for molecular docking verification. Results Eighty-two active compounds, including Xylostosidine, Arctiin, ZINC12153652 and ZINC338038, were screened from Compound Houttuyniae mixture. The key targets involved 128 targets, including MAPK1, MAPK3, MAPK8, MAPK14, TP53, TNF, and IL6. The HIF-1 signaling, VEGF signaling, TNF signaling and another 127 signaling pathways associated with COVID-19 were affected ( P < 0.05). From the results of molecular docking, the binding ability between the selected active components and the core targets was strong. Conclusion Through the combination of network pharmacology and molecular docking technology, this study revealed that the therapeutic effect of Compound Houttuynia mixture on COVID-19 was realized through multiple components, multiple targets and multiple pathways, which provided a certain scientific basis of the clinical application of Compound Houttuynia mixture.

Structure Identification and Evaluation of Chemical Components from the Flos Sophorae Immaturus for Inhibitory Effects against HepG2

2021 ◽  
Vol 19 (4) ◽  
pp. 452-457
Author(s):  
Zhaohui Xue ◽  
Weichen Song ◽  
Xin Gao ◽  
Wancong Yu ◽  
Xiaonan Hou ◽  
...  
Keyword(s):  
Standard Of Care ◽  
Scientific Basis ◽  
Added Value ◽  
Structure Identification ◽  
Chemical Components ◽  
Active Components ◽  
Sophora Japonica ◽  
Beneficial Effects ◽  
Cell Proliferation Inhibition ◽  
Inhibition Potency

Flos Sophorae Immaturus, dried buds of Sophora japonica, exhibits multiple pharmacologic activities including anti-inflammatory, antiaging, and antiallergy effects as well as adjunct to standard of care cancer therapy. However, active components responsible for beneficial effects in cancer are not known. In this study, Flos Sophorae Immaturus was obtained by ethanol extraction, and eight components were purified, identified, and tested for HepG2 cell proliferation inhibition potency. The compounds identified to be the most effective inhibitors were campherol > isorhamnetin > quercetin. These data provide theoretical and scientific basis for the further development, utilization, and the promotion of added value of Flos Sophorae Immaturus.

scholarly journals Studies on the Active Components and Antioxidant Activity of the Extracts from Different Parts of Bamboo

2013 ◽  
Vol 25 (11) ◽  
pp. 6354-6360 ◽  
Author(s):  
Yu-Xian Li ◽  
Fang-Rong Cheng ◽  
Yong-Chun Jin ◽  
Ke Yuan
Keyword(s):  
Antioxidant Activity ◽  
Active Components ◽  
Different Parts

In Vitro Investigation of Anti-Diabetic Effect of Taxus cuspidate Extracts by Ultrasound Assisted Method

2012 ◽  
Vol 40 (06) ◽  
pp. 1205-1215 ◽  
Author(s):  
Linda Xiao ◽  
Wei-Guo Lao ◽  
Yi Tan ◽  
Xianqin Qu
Keyword(s):  
Glucose Uptake ◽  
Active Compound ◽  
Medicinal Herbs ◽  
Chinese Medicinal Herbs ◽  
Active Components ◽  
In Vitro Investigation ◽  
Ultrasound Assisted ◽  
Taxus Cuspidate

Extracting active components from Chinese medicinal herbs efficiently is a key step in the investigation of their pharmacological effects and modes of action. In this project, we compared the ultrasound-assisted method and the conventional solvent method for extracting the active compound of Taxus cuspidate (dong bei hong dou shan). Through the analysis of various extractions with a quadruple time-of-fight (Q-TOF) LC/MS, we demonstrated that the ultrasound-assisted method reduced solvent consumption and had shorter extraction time, while the extraction yields of the active compound (taxol) were equivalent to or even higher than those obtained with the conventional solvent extraction method. Through the comparison of Taxus cuspidate extracts (TCEs) with different concentrations of acetone and ethanol, we proved that 50% ethanol was an optimal solvent for extracting taxol from Taxus cuspidate. Based on traditional Chinese medicine (TCM) literature, we further determined whether TCEs possess antidiabetic effects by testing glucose uptake in 3T3-L1 adipocytes treated with TCEs from Taxus cuspidate bark and twigs under insulin stimulation (100 nM). The results showed that neither taxol (10 μg/ml) nor TCEs (1 and 0.1 mg/ml) changed glucose uptake significantly compared with insulin alone. This study demonstrated that the ultrasound-assisted method with 50% ethanol is a highly efficient approach for extracting Taxus cuspidate, which may be applicable for extraction of other Chinese medicinal herbs. Extracts of Taxus cuspidate bark and twigs had no effect on insulin stimulated-glucose uptake in vitro. This result conflicts with the description in TCM literature. Further in vivo study to clarify Taxus cuspidate's metabolic actions is necessary.

scholarly journals Network Pharmacology‐Based Study on the Active Component and Mechanism of the Anti-Gastric-Cancer Effect of Herba Sarcandrae

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Li Han ◽  
Ying Han
Keyword(s):  
Gastric Cancer ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Target Genes ◽  
Scientific Basis ◽  
Gene Expression Omnibus ◽  
Network Pharmacology ◽  
Chemical Components ◽  
Active Components ◽  
Pathway Network

Background. Herba Sarcandrae is used in the clinical practice of traditional Chinese medicine to deal with gastric cancer. However, there are few studies on its precise mechanism. Method. In this study, a network pharmacological approach was utilized to construct a molecular/target/pathway molecular regulatory network for the anti-gastric-cancer effect of Herba Sarcandrae. The active components of Herba Sarcandrae and their potential mechanisms were explored. Chemical components of the Herba Sarcandrae were identified through a database, and they were evaluated and screened based on oral bioavailability and drug similarity. Results. Genes related to gastric cancer were found in the Gene Expression Omnibus (GEO) database, and gene targets related to anti-gastric-cancer were chosen by comparison. Using annotation, visualization, and a comprehensive discovery database, the function and related pathways of target genes were analyzed and screened. Cytoscape software was utilized to construct a component/target/pathway network for the antitumor effect of Herba Sarcandrae. Finally, 6 drug ingredients and 29 target genes related to gastric cancer were detected. IL-17 signaling pathway, NF-kappa B signaling pathway, and other signaling pathways were significantly enriched. Many signaling pathways that directly act on tumors and indirect pathways inhibit the development of gastric cancer. Conclusion. This study provides a scientific basis for further elucidating the mechanism of the anti-gastric-cancer effect of Herba Sarcandrae.

Rapid screening of active components group with Topoisomerase I inhibitory activity in Sophora alopecuroides L. based on ultrafiltration coupled with UPLC-QTOF-MS

2021 ◽  
Vol 22 ◽  
Author(s):  
Lin Zhang ◽  
Xiaoying Yin ◽  
Xi Wan ◽  
Yun Sun ◽  
Menghui Cao ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Topoisomerase I ◽  
Molecular Mechanisms ◽  
Ultra Performance Liquid Chromatography ◽  
Rapid Screening ◽  
Active Components ◽  
Pharmacological Mechanism ◽  
Flight Mass Spectrometry ◽  
Sophora Alopecuroides

Background: Topoisomerase I (Topo I) is a key target of many antitumor drugs in vivo. Alkaloids in Sophora alopecuroides L. can reportedly inhibit Topo I activity, but the pharmacodynamic material basis has not yet been determined. Objective: The objective of this study is to rapidly identify active components group which inhibit Topo I in S. alopecuroides L. Methods: Affinity ultrafiltration-ultra-performance liquid chromatography-quadrupole time of flight-mass spectrometry (UF-UPLC-QTOF-MS) screening system based on Topo I protein was established to screen and isolate a total alkaloid fraction in S. alopecuroides L. Topo I inhibitory activity and anti-tuomor proliferation activity of the screened components were evaluated, and their molecular mechanisms were studied. Results: Six compounds bound specifically to Topo I were obtained. Further screening showed that matrine, cytisine, and sophoridine presented higher inhibitory activity on Topo I and were able to inhibit the proliferation of breast cancer MDA-MB-468 cells with IC50 values of 9.40 ± 1.12 mM, 17.4 ± 2.20 mM and 10.4 ± 1.37 mM, respectively. To the best of our knowledge, their dual molecular mechanisms against Topo I have been discussed here for the first time: (1) stabilization of Topo I-DNA complex and (2) inhibition or blocking of Topo I binding to DNA. Conclusion: Matrine, cytisine, and sophoridine from S. alopecuroides L. were defined as the active components group with Topo I inhibitory activity and their pharmacological mechanism was confirmed, which provided an important base for further research and development of antitumor components fromS. alopecuroides L.

scholarly journals Isolation and Identification of Benzochroman and Acylglycerols from Massa Medicata Fermentata and Their Inhibitory Effects on LPS-Stimulated Cytokine Production in Bone Marrow-Derived Dendritic Cells

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2400 ◽  
Author(s):  
Ya Sun ◽  
Seo Yang ◽  
Young-Sang Koh ◽  
Young Kim ◽  
Wei Li
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Scientific Basis ◽  
Inhibitory Effects ◽  
Active Components ◽  
Isolation And Identification ◽  
Chemical Structures ◽  
Tnf Α ◽  
Ic50 Values ◽  
Reported Data

Massa Medicata Fermentata (MMF), known as Shenqu, is an important traditional Chinese medicine widely used to treat indigestion, vomiting, and diarrhea. In this study, a new benzochroman, 3(S)-3,4-dihydro-5,10-di-β-d-glucopyranoside-2,2-dimethyl-2H-naphtho(2,3-b)pyran-3-ol (1), and five known galactosyl acylglycerols (2–6) were isolated from a methanol extract from MMF. In addition, their chemical structures were determined by chemical and spectroscopic methods, which were compared with the previously reported data. Furthermore, the effects of isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 1–3 exhibited significant inhibitory effects on the LPS-induced production of IL-6 and IL-12 p40, with IC50 values ranging from 1.6 to 10.2 μM. Compounds 2 and 3 also exhibited strong inhibitory effects on the LPS-stimulated production of TNF-α with IC50 values of 12.0 and 11.2 μM, respectively. The results might provide a scientific basis for the development of the active components in MMF, as well as for novel anti-inflammatory agents.

Sign in / Sign up

Export Citation Format

Share Document