Microbial Oil Production from Lignocellulosic Biomass – Recent Development and Prospect

2014 ◽  
Vol 541-542 ◽  
pp. 397-403
Author(s):  
Zhang Nan Lin ◽  
Hong Juan Liu ◽  
Zhi Qin Wang ◽  
Jia Nan Zhang

Microbial oil is one of the ideal raw materials for biodiesel production because of its rapid reproduction and less influence by the climate and season variation. However, the high cost is one of the key issues that restricted its production in a large-scale. Lignocellulosic biomass, the cheap and renewable resource, might be the best raw material for microbial oil production by oleaginous microorganisms. Recent development on the microbial oil production from lignocellulosic biomass was summarized in this paper. Furthermore, the challenges and application potential of microbial oil were prospected.

Energies ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1473
Author(s):  
Marco Castellini ◽  
Stefano Ubertini ◽  
Diego Barletta ◽  
Ilaria Baffo ◽  
Pietro Buzzini ◽  
...  

Today one of the most interesting ways to produce biodiesel is based on the use of oleaginous microorganisms, which can accumulate microbial oil with a composition similar to vegetable oils. In this paper, we present a thermo-chemical numerical model of the yeast biodiesel production process, considering cardoon stalks as raw material. The simulation is performed subdividing the process into the following sections: steam explosion pre-treatment, enzymatic hydrolysis, lipid production, lipid extraction, and alkali-catalyzed transesterification. Numerical results show that 406.4 t of biodiesel can be produced starting from 10,000 t of lignocellulosic biomass. An economic analysis indicates a biodiesel production cost of 12.8 USD/kg, thus suggesting the need to increase the capacity plant and the lipid yield to make the project economically attractive. In this regard, a sensitivity analysis is also performed considering an ideal lipid yield of 22% and 100,000 t of lignocellulosic biomass. The biodiesel production costs related to these new scenarios are 7.88 and 5.91 USD/kg, respectively. The large capacity plant combined with a great lipid yield in the fermentation stage shows a biodiesel production cost of 3.63 USD/kg making the product competitive on the current market of biofuels by microbial oil.


2011 ◽  
Vol 61 ◽  
pp. 1-19 ◽  
Author(s):  
Tristan Carter

AbstractThis paper reviews 50 years of obsidian studies at Neolithic Çatalhöyük in the Konya plain, central Anatolia. A number of key issues are addressed: (1) the source of the site's raw materials, the means and forms by which the obsidian was introduced to the site and the role of Çatalhöyük in the supra-regional dissemination of these raw materials; (2) the alleged gender associations of certain obsidian goods in the burial record and beyond; (3) a more general consideration of the social significance of the circulation and consumption of obsidian at the site, including the phenomena of hoarding and gifting, plus the important role of projectiles in the creation of social identities and various forms of ritual behaviour, not least the termination of the life of a building/individual; (4) the technotypological and raw material variability through time; (5) the use of obsidian in daily practice and craft-working.


Author(s):  
K. Malins ◽  
V. Kampars ◽  
R. Kampare ◽  
T. Rusakova

The transesterification of vegetable oil using various kinds of alcohols is a simple and efficient renewable fuel synthesis technique. Products obtained by modifying natural triglycerides in transesterification reaction substitute fossil fuels and mineral oils. Currently the most significant is the biodiesel, a mixture of fatty acid methyl esters, which is obtained in a reaction with methanol, which in turn is obtained from fossil raw materials. In biodiesel production it would be more appropriate to use alcohols which can be obtained from renewable local raw materials. Ethanol rouses interest as a possible reagent, however, its production locally is based on the use of grain and therefore competes with food production so it would implicitly cause increase in food prices. Another raw material option is alcohols that can be obtained from furfurole. Furfurole is obtained in dehydration process from pentose sugars which can be extracted from crop straw, husk and other residues of agricultural production. From furfurole the tetrahydrofurfuryl alcohol (THFA), a raw material for biodiesel, can be produced. By transesterifying rapeseed oil with THFA it would be possible to obtain completely renewable biodiesel with properties very close to diesel [2-4]. With the purpose of developing the synthesis of such fuel, in this work a three-stage synthesis of rapeseed oil tetrahydrofurfurylesters (ROTHFE) in sulphuric acid presence has been performed, achieving product with purity over 98%. The most important qualitative factors of ROTHFE have been determined - cold filter plugging point, cetane number, water content, Iodine value, phosphorus content, density, viscosity and oxidative stability.


PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11525
Author(s):  
Hong Li ◽  
Jun Tan ◽  
Yun Mu ◽  
Jianfeng Gao

Chlorella has become an important raw material for biodiesel production in recent years, and Chlorella sp. TLD6B, a species with high lipid concentrations and high salt and drought tolerance, has been cultivated on a large scale. To explore the lipid accumulation of Chlorella sp. TLD6B and its relationship to external NaCl concentrations, we performed physiological measurements and genome-wide gene expression profiling under different levels of salt stress. Chlorella sp. TLD6B was able to tolerate high levels of salt stress (0.8 M NaCl addition). Lipid concentrations initially increased and then decreased as salt stress increased and were highest under the addition of 0.2 M NaCl. Comparative transcriptomic analysis revealed that salt stress enhanced the expression of genes related to sugar metabolism and fatty acid biosynthesis (the ACCases BC and BCCP, KAS II, and GPDHs involved in TAG synthesis), thereby promoting lipid accumulation under the addition of 0.2 M NaCl. However, high salinity inhibited cell growth. Expression of three SADs, whose encoded products function in unsaturated fatty acid biosynthesis, was up-regulated under high salinity (0.8 M NaCl addition). This research clarifies the relationship between salt tolerance and lipid accumulation and promotes the utilization of Chlorella sp. TLD6B.


Author(s):  
Asep Bayu Dani Nandiyanto ◽  
Nissa Nur Azizah ◽  
Gabriela Chelvina Santiuly Girsang

Corncob is usually disposed of directly as waste, creating problems in the environment, while it can be converted into valuable materials. This research aimed to evaluate the literature review on briquette production from agricultural waste (using non-binder and cold press with a binder) and the current works on techno-economic analysis, to propose an optimal design for the production of briquette from corncob waste, and to perform a techno-economic analysis based on the selected optimal processing method. The engineering perspective based on stoichiometry and mass balance showed the potential corncob briquette manufacture in both home and large scales due to the possible use of inexpensive and commercially available equipment and raw materials. The economic perspective [based on several economic evaluation factors (i.e., gross profit margin, payback period, break-even point, cumulative net present value, return of investment, internal rate return, and profitability index) under ideal and non-ideal conditions by considering internal (i.e., sales, raw materials, utilities, and variable cost) and external aspects (i.e., tax)] confirmed the prospective development of the project in the large-scale production with a lifetime of more than 18 years. The main issue in the project is the raw material (i.e. tapioca flour), giving the most impact on the project’s feasibility. Even in severe conditions, the project is feasible. The great endurance was also confirmed in the case of a higher tax rate. This study demonstrates the importance of producing corncob-based briquettes for improving the economic value and giving alternatives for problem solvers in the utilization of agricultural waste.


Author(s):  
Daniel Sena MARINS ◽  
Marcos Vinícius Oliveira CARDOSO ◽  
Mara Eliza SANTOS ◽  
Jeferson MASSINHAN

Demand for diversified biodiesel feedstocks is high and increasing, but few are viable for large-scale production, and many of those selected compete with other sectors of the chemical industry. To improve energy and environmental sustainability, fatty acids from waste oils that are improperly disposed of and pollute the environment can be used for transesterification reactions. However, they need treatment to achieve high conversion rates. In this context, the aim of this work was to perform and analyze the treatment of residual frying oil with the evaporation and entrainment process, aiming at its use as raw material to obtain biodiesel (methyl esters) by a transesterification reaction. The physicochemical properties of the residual oil after treatment were characterized by moisture content, pH and the acidity, saponification, iodine, and peroxide index. The conversion rate of the residual oil to methyl esters was determined by 1H NMR analysis. After the treatment, the method of analysis of variance showed that the oil obtained a significant reduction of the saponification, iodine, peroxide and acidity indexes, being the acidity reduced from 9.36 to 7.85 mg KOH g-1. The moisture content of 0.733% and elevation of pH to 8.0. The conversion rate of fatty acid biodiesel of residual oil was 79.3 %, lower value of standards norms (ASTM, 2005; EN, 2008; ANP, 2014), showing that the assigned methodology for frying residual oil is inefficient in biodiesel production.


2018 ◽  
Vol 56 ◽  
pp. 04003
Author(s):  
Sergei Tkach

The article deals with the problems of mineral raw material losses of quality and quantity management in developing of large-scale complex-structure and composition deposits of solid minerals. It is shown that a very high degree of mining and geological conditions variability in time and space for the development of mining units is typical for such deposits. This significantly complicates the qualitative and quantitative operating losses setting and accounting of mineral raw materials during its extraction in the framework of existing general and industry regulatory documents. Conceptual principles for face-by-face operational setting of losses and impoverishment of minerals for the conditions of bulk mining of complex-structure deposits with the formation of gross mining flow with economically feasible and specified level of quality (the content of commercial and harmful components) are stated. These principles generally do not contradict effective instructions main provisions and are made to minimize the total operating losses during the processing of several mine sections (faces).


Minerals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 174 ◽  
Author(s):  
Peter Blistan ◽  
Stanislav Jacko ◽  
Ľudovít Kovanič ◽  
Julián Kondela ◽  
Katarína Pukanská ◽  
...  

A frequently recurring problem in the extraction of mineral resources (especially heterogeneous mineral resources) is the rapid operative determination of the extracted quantity of raw material in a surface quarry. This paper deals with testing and analyzing the possibility of using unconventional methods such as digital close-range photogrammetry and terrestrial laser scanning in the process of determining the bulk density of raw material under in situ conditions. A model example of a heterogeneous deposit is the perlite deposit Lehôtka pod Brehmi (Slovakia). Classical laboratory methods for determining bulk density were used to verify the results of the in situ method of bulk density determination. Two large-scale samples (probes) with an approximate volume of 7 m3 and 9 m3 were realized in situ. 6 point samples (LITH) were taken for laboratory determination. By terrestrial laser scanning (TLS) measurement from 2 scanning stations, point clouds with approximately 163,000/143,000 points were obtained for each probe. For Structure-from-Motion (SfM) photogrammetry, 49/55 images were acquired for both probes, with final point clouds containing approximately 155,000/141,000 points. Subsequently, the bulk densities of the bulk samples were determined by the calculation from in situ measurements by TLS and SfM photogrammetry. Comparison of results of the field in situ measurements (1841 kg∙m−3) and laboratory measurements (1756 kg∙m−3) showed only a 4.5% difference in results between the two methods for determining the density of heterogeneous raw materials, confirming the accuracy of the used in situ methods. For the determination of the loosening coefficient, the material from both large-scale samples was transferred on a horizontal surface. Their volumes were determined by TLS. The loosening coefficient for the raw material of 1.38 was calculated from the resulting values.


2015 ◽  
Vol 659 ◽  
pp. 216-220 ◽  
Author(s):  
Achanai Buasri ◽  
Thaweethong Inkaew ◽  
Laorrut Kodephun ◽  
Wipada Yenying ◽  
Vorrada Loryuenyong

The use of waste materials for producing biodiesel via transesterification has been of recent interest. In this study, the pork bone was used as the raw materials for natural hydroxyapatite (NHAp) catalyst. The calcination of animal bone was conducted at 900 °C for 2 h. The raw material and the resulting heterogeneous catalyst were characterized using X-ray diffraction (XRD), X-ray fluorescence (XRF), scanning electron microscopy (SEM) and the Brunauer-Emmett-Teller (BET) method. The effects of reaction time, microwave power, methanol/oil molar ratio, catalyst loading and reusability of catalyst were systematically investigated. The optimum conditions, which yielded a conversion of oil of nearly 94%, were reaction time 5 min and microwave power 800 W. The results indicated that the NHAp catalysts derived from pork bone showed good reusability and had high potential to be used as biodiesel production catalysts under microwave-assisted transesterification of Jatropha Curcas oil with methanol.


Author(s):  
Luthfi Assadad ◽  
Bagus Sediadi Bandol Utomo ◽  
Rodiah Nurbaya Sari

Biofuel is one of alternative fossil fuel, in which the raw materials come from biological resources.One of the raw materials for biofuel production is microalgae. Microalgae grows rapidly, does notcompete with food for humans, and needs small areas to cultivate. Utilization of microalgae forbiofuel research nowadays is focusing on biodiesel production, but actually microalgae can beused to produce other biofuels such as bioethanol. The carbohydrate content of the microalgaecan be converted into glucose and fermented into alcohol. Carbohydrate content of the microalgaeis about 5.0–67.9%, which could produce bioethanol up to 38%. A harmony between bioethanoland biodiesel production from microalgae is needed for the optimum utilization of microalgae.Bioethanol production from microalgae can be done using de-oiled microalgae.


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