A Novel Way of Separation and Preparation Noncaffeine Tea Polyphenols from Green Tea Waste

2012 ◽  
Vol 550-553 ◽  
pp. 1875-1880 ◽  
Author(s):  
He Ping Tan ◽  
Huai Ping Li ◽  
Hang Song ◽  
Wen Ping Xu ◽  
Chi Guan ◽  
...  
Keyword(s):  
Green Tea ◽  
Column Chromatography ◽  
High Performance ◽  
Ethanol Solution ◽  
Tea Polyphenols ◽  
Raw Material ◽  
Environment Friendly ◽  
Tea Waste ◽  
One Step ◽  
The Stability

A simple, clean and economical way of separation and preparation noncaffeine tea polyphenols from green tea waste in one-step by Sephadex LH-20 column chromatography was developed. 20% (v/v) ethanol solution was used for removing caffeine and other components in the first stepwise elution, and tea polyphenols was eluted with 60% (v/v) ethanol solution by using reversed flow in the second stepwise elution. The yield of tea polyphenols above 11% with the purity about 98% and the content of catechins more than 91%, and the content of caffeine in tea polyphenols have not been detected by high-performance liquid chromatography. The recovery of tea polyphenols by column chromatography were about 86%, and the stability of preparative way is very good. Compared with conventional extraction and decaffeination ways, this way has more excellent advantages, such as cheaper raw material, no toxic residues, higher yield, commercially feasible and environment friendly.

scholarly journals Development and Validation of a Micellar High-Performance Liquid Chromatographic Method for Determination of Risedronate in Raw Material and in a Pharmaceutical Formulation: Application to Stability Studies

2010 ◽  
Vol 93 (4) ◽  
pp. 1228-1235 ◽  
Author(s):  
Mohamed Walash ◽  
Mohamed Metwally ◽  
Manal Eid ◽  
Rania El-Shaheny
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Sodium Dodecyl ◽  
Oxidative Degradation ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Raw Material ◽  
Forced Degradation ◽  
The Stability ◽  
Liquid Chromatographic

Abstract A micellar HPLC method was developed for analysis of the antiosteoporosis drug risedronate. The analysis was carried out using a 250 4.6 mm id, 5 m particle size C18 Waters Symmetry column. The mobile phase consisted of 0.02 M sodium dodecyl sulfate + 0.3 triethylamine + 10 n-propanol, prepared in 0.02 M orthophosphoric acid. The pH of the mobile phase was adjusted to pH 6.0, and it was pumped at a flow rate of 0.7 mL/min with UV detection at 262 nm. The method showed good linearity in the range of 280 g/mL, with an LOD of 0.40 g/mL (1.31 106 M) and an LOQ of 1.21 g/mL. The suggested method was successfully applied for the analysis of risedronate in raw material and a tablet formulation, with average recoveries of 99.91 1.30 and 101.52 0.30, respectively. The stability-indicating capability of the proposed method was proved using forced degradation. By changing the pH of the mobile phase to 4.0, the oxidative degradation product could be separated from risedronate.

Recovery of Tea Polyphenols from Green Tea Waste by Liquid-Liquid Extraction

2011 ◽  
Vol 396-398 ◽  
pp. 1592-1595
Author(s):  
Zhong Jian Li ◽  
Zhe Wei ◽  
Wei Xiao ◽  
Jun Wang ◽  
Fu An Wu
Keyword(s):  
Distribution Coefficient ◽  
Green Tea ◽  
Ph Value ◽  
New Technology ◽  
Selective Extraction ◽  
Extraction Process ◽  
Tea Polyphenols ◽  
Extraction Temperature ◽  
Tea Waste ◽  
Equilibrium Data

Tea (Camellia sinensis L.) has became one of the most consumed beverages in the world in the past five thousand years, and tea polyphenols (TPs) are important organic acids widely used in chemical, pharmaceutical, food and other industries, which have been shown to exhibit various biological and pharmacological properties. In order to look for new technology of downstream process for TPs separation from green tea waste, selective extraction of TPs with 12 varieties of solvents were carried out, the distribution coefficient and equilibrium experiments at various temperatures were described by extraction isotherms model. The results indicated that the optimum solvent conditions were as follow: extractent was glyceryl triacetate, extraction temperature range was 20~30°C, pH value of crude TPs and reextractant were 3.17 and 9, respectively. Equilibrium data of TPs were successfully fitted to Henry isotherm, the distribution coefficient decreases with the increasing of the temperature, which showing an exothermic adsorption process. The data obtained is useful in the designing of solvent extraction process for the recovery of TPs from green tea waste.

Study on the Extraction of Natural Essence from Green Tea Leaf by Supercritical CO2

2013 ◽  
Vol 781-784 ◽  
pp. 869-874 ◽  
Author(s):  
He Ping Tan ◽  
Huai Ping Li ◽  
Chi Guan ◽  
Hang Song ◽  
Yang Xu ◽  
...  
Keyword(s):  
Green Tea ◽  
Biochemical Composition ◽  
High Performance ◽  
Raw Materials ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Before And After ◽  
Static Extraction ◽  
Tea Leaf ◽  
Aroma Components

A simple and clean way of extraction of natural essence from green tea leaf by supercritical CO2 was developed. The optimum extraction conditions to produce the high-quailty natural essence were found: loading about 100g/L, operating at 5000 psi and 50 °C, and separation kettle temperature 75 °C, static extraction three times with 40 min every time, the yield of natural essence was 0.5%. The product was analyzed by gas chromatography-mass spectrometry (GC-MS) show that dimethylsulfide was the characteristic aroma components of the natural essence with more than 50 kinds of alcohols, ketones, aldehydes, ethers and esters etc.. There were only little other biochemical composition of raw material and 0.4% caffeine in the product detected by high-performance liquid chromatography (HPLC), and the biochemical composition in the raw material were almost no loss before and after extracted by supercritical CO2, it indicated that the specificity of the method for extraction tea natural essence is very strong, and propitious to improve the secondary utilization of the tea raw materials.

scholarly journals Stability of Tilo® tablets formulation obtained from dry extract of Justice pectoralis Jacq.

2015 ◽  
Vol 51 (1) ◽  
pp. 193-202
Author(s):  
Jorge E. Rodríguez-Chanfrau ◽  
Yanier Núñez-Figueredo ◽  
Viviana Fuste-Moreno
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Extract ◽  
High Performance ◽  
Raw Material ◽  
Biological Stability ◽  
Stability Studies ◽  
Dry Extract ◽  
Pharmaceutical Tablets ◽  
The Stability

Justicia pectoralis Jacq., Acanthaceae, is a herb known popularly in Cuba as Tilo and used traditionally as sedative. The development in a solid pharmaceutical (Tablets 100 mg) using dry extract of Justicia pectolaris aqueous extract is of interest for the development of phytomedicines, which uses this active raw material. The aim of the present study was to carry out chemical and biological stability studies to the formulation. A method of coumarin determination by High Performance Liquid Chromatography (HPLC) was used and validated. The stability studies during different periods of time (24 months) showed a stability of the product stored at 32 ± 2 °C, and protected of the light.

scholarly journals The study of the flavonoid composition of the herb of monarda fistulosa leaves

2020 ◽  
Vol 19 (5-6) ◽  
pp. 135-142
Author(s):  
Anastasiya S. Lapina ◽  
Vladimir A. Kurkin
Keyword(s):  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
High Performance ◽  
Nmr Spectra ◽  
Uv Spectroscopy ◽  
Botanical Garden ◽  
Raw Material ◽  
Monarda Fistulosa ◽  
Flavonoid Composition

Actuality. The herb of Monarda fistulosa L. contains the essential oil and flavonoids, which are of the greatest interest in terms of pharmacological properties. The herb of Monarda fistulosa L. contains a number of flavonoids (rutin, hesperidin, diosmin, quercetin, luteolin, luteolin-7-O-glucoside, naringenin, catechin), however, the literature on the flavonoid composition of this plant is contradictory. In turn, this gives rise to a variety of approaches to standartization of the herb Monarda fistulosa L. The aim is to study the flavonoid composition of Monarda fistulosa L. by means of column chromatography and high performance liquid chromatography (HPLC). Materials and methods. The herb Monarda fistulosa L. was collected during the flowering period at the Botanical Garden of Samara University in 2018. Individual substances were isolated by means of column chromatography. Bruker DRX 500 instrument (126.76 MHz) was used to determine 13C NMR spectra, and Bruker AM 300 instrument (300 MHz) was used to determine 1H NMR spectra. Mass spectra were recorded by mass-spectrometer Kratos MS-30, UV spectra were recorded by means of the spectrophotometer Specord 40 (Analytik Jena). HPLC analysis was carried out with the use of chromatograph Milichrom-6 (NPO Nauchpribor). Results. For the first time column chromatography by means of 1H-NMR, 13C-NMR, UV spectroscopy, mass spectrometry allowed to isolate from Monarda fistulosa L. and characterize the following flavonoids: isorhoifolin (7-O-rutinoside of apigenin), linarin (7-O-rutinoside of acacetin), didymin (7-O-rutinoside of isosakuranetin), monardoside (5-O-rutinoside of apigenin), apigenin, acacetin. The composition of flavonoids was studied by means of HPLC method and isolated individual substances. Conclusion. The results can be used to develop a pharmacopoeial monograph for a new type of raw material Monarda fistulosa herb.

scholarly journals Determination of catechins in green tea leaves by HPLC compared to spectrophotometry

2021 ◽  
Vol 19 (3(75)) ◽  
pp. 28-33
Author(s):  
Oleksandr Yu. Maslov ◽  
Mykola A. Komisarenko ◽  
Yulia S. Kolisnyk ◽  
Tatyana A. Kostina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Green Tea ◽  
High Performance ◽  
Raw Material ◽  
Qualitative Composition ◽  
Tea Leaves ◽  
Quantitative Content ◽  
Significant Difference ◽  
Green Tea Leaves

Aim. To study the qualitative composition, the quantitative content of catechins in green tea leaves and compare the data obtained with those evaluated by spectrophotometry.Materials and methods. Green tea leaves used for the analysis were collected in Anhui Province, China. The extract for the HPLC analysis was obtained by the maceration method with 60 % ethanol twice in the raw material/extractant ratio of 1 : 20. In the case of the spectrophotometric analysis, green tea leaves were extracted with 70 % ethanol twice by the maceration method in the raw material/extractant ratio of 1 : 20. The analysis of the extract from green tea leaves was performed by high performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with a SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversed phase, C18 modified silica gel, length – 150 mm, diameter – 4.6 mm, particles size – 5 μm). Substances in the extract were identified by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. Spectrophotometric measurements were carried out using a UV-1000 single beam spectrophotometer (China) with the pair of S90-309Q quartz square cells.Results and discussion. Using high performance liquid chromatography 5 catechins were identified. Among them epigallocatechin-3-O-gallate (10.85 %) predominated, while catechin (0.61 %) had the lowest concentration. The total amount of catechins in green tea leaves was 30.56 and 24.79 % by HPLC and spectrophotometry, respectively. The F- and t-tests showed that there was no significant difference between the results of HPLC and spectrophotometry.Conclusions. The qualitative composition and the quantitative content of catechins have been determined in the extract from green tea leaves by high performance liquid chromatography and spectrophotometry. Both HPLC and spectrophotometric methods can be used to determine the total catechin content in green tea leaves. The high content of catechins makes the extract promising for further study and creation of new herbal medicinal products and dietary supplements. The results obtained will be used for standardization of green tea leaves and for future pharmacological research of its extract.

Rotigotine: A Review of Analytical Methods for the Raw Material, Pharmaceutical Formulations, and Its Impurities

2020 ◽  
Author(s):  
Thamara de Carvalho Mendes ◽  
Eduardo Costa Pinto ◽  
Lucio Mendes Cabral ◽  
Valeria Pereira de Sousa
Keyword(s):  
Analytical Methods ◽  
High Performance ◽  
Pharmaceutical Formulations ◽  
The United States ◽  
Raw Material ◽  
Forced Degradation ◽  
Pharmaceutical Dosage Forms ◽  
Need To Evaluate ◽  
Degradation Studies ◽  
The Stability

Abstract Background Rotigotine is a dopaminergic agonist developed for the treatment of Parkinson’s disease and restless leg syndrome. The pure levorotatory enantiomer is marketed in several countries as a transdermal patch. Reports of oxidation and instability in a previous formulation indicate the need to evaluate impurities in both the raw material and pharmaceutical dosage forms of rotigotine to ensure product quality. Objective This review examines the main analytical methods for analyzing rotigotine in raw material and its transdermal patches with the aim of assisting the development of new pharmaceutical formulations and stability studies. Methods Analytical methods based on high-performance liquid chromatography for rotigotine from pharmacopoeias and literature were evaluated. A comparison was made between the methods found in the literature and official rotigotine monographs described by the United States, European, and British Pharmacopoeias, including a discussion of their acceptance limits for impurities related to the drug. The different impurities from the synthesis processes and degradation studies of rotigotine were also evaluated, as well as the main articles that describe methods for assessing their chiral purity. Results Qualified and unofficial official impurities found in forced degradation studies were verified. The methods presented show adequate specificity and selectivity in determining the drug in the presence of its impurities. Conclusions The approached methods are promising, but more detailed studies on the stability of rotigotine are still lacking, mainly in the pharmacokinetic and toxicological characterization of its impurities.

Dietary Vitamin and Stability of Oral Anticoagulation: Proposal of a Diet with Constant Vitamin K1 Content

1997 ◽  
Vol 77 (03) ◽  
pp. 504-509 ◽  
Author(s):  
Sarah L Booth ◽  
Jacqueline M Charnley ◽  
James A Sadowski ◽  
Edward Saltzman ◽  
Edwin G Bovill ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Vitamin K ◽  
High Performance ◽  
Case Reports ◽  
Food Composition ◽  
Dietary Guidelines ◽  
Dietary Vitamin ◽  
Vitamin K1 ◽  
The Stability ◽  
The Impact

SummaryCase reports cited in Medline or Biological Abstracts (1966-1996) were reviewed to evaluate the impact of vitamin K1 dietary intake on the stability of anticoagulant control in patients using coumarin derivatives. Reported nutrient-drug interactions cannot always be explained by the vitamin K1 content of the food items. However, metabolic data indicate that a consistent dietary intake of vitamin K is important to attain a daily equilibrium in vitamin K status. We report a diet that provides a stable intake of vitamin K1, equivalent to the current U.S. Recommended Dietary Allowance, using food composition data derived from high-performance liquid chromatography. Inconsistencies in the published literature indicate that prospective clinical studies should be undertaken to clarify the putative dietary vitamin K1-coumarin interaction. The dietary guidelines reported here may be used in such studies.

The Utilization of Ftir (Fourier Transform Infra-Red) Method Combined with Chemometrics For Authentication of Indonesian Coffee Powder

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Firmansyah A. ◽  
Winingsih W. ◽  
Soebara Y S
Keyword(s):  
Fourier Transform ◽  
Natural Product ◽  
High Performance ◽  
Principal Component ◽  
Raw Material ◽  
Attenuated Total Reflectance ◽  
Coffee Bean ◽  
Product Analysis ◽  
Total Reflectance ◽  
And Cluster Analysis

Analysis of natural product remain challenging issues for analytical chemist, since natural products are complicated system of mixture. The most popular methods of choice used for quality control of raw material and finished product are high performance liquid chromatography (HPLC), gas chromatography (GC) and mass spectrometry (MS). The utilization of FTIR-ATR (Fourier Transform Infrared-Attenuated Total Reflectance) method in natural product analysis is still limited. This study attempts to expand the use of FTIR spectroscopy in authenticating Indonesian coffee powder.The coffee samples studied were taken from nine regions in Indonesia, namely Aceh Gayo, Flores, Kintamani, Mandheling, Papua, Sidikalang, Toraja, Kerinci and Lampung.The samples in the form of coffee bean from various regions were powdered . The next step conducted was to determine the spectrum using the FTIR-ATR (Attenuated Total Reflectance) using ZnSe crystal of 8000 resolution. Spectrum samples, then, were analyzed using chemometrics. The utilized chemometric model was the principal component analysis (PCA) and cluster analysis (CA). Based on the chemometric analysis, there are similarities between Aceh Gayo coffee with Toraja coffee, Mandailing coffee, Kintamani coffee and Flores coffee. Sidikalang coffee has a similarity to Flores coffee; Papua coffee has a similarity to Sidikalang coffee; Lampung coffee has a similarity to Sidikalang coffee, while Kerinci coffee has a similarity to Papua coffee.

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