scholarly journals A Review of Enzymatic Transesterification of Microalgal Oil-Based Biodiesel Using Supercritical Technology

2011 ◽  
Vol 2011 ◽  
pp. 1-25 ◽  
Author(s):  
Hanifa Taher ◽  
Sulaiman Al-Zuhair ◽  
Ali H. Al-Marzouqi ◽  
Yousef Haik ◽  
Mohammed M. Farid
Keyword(s):  
Solvent Extraction ◽  
New Technologies ◽  
Immobilized Lipase ◽  
Biodiesel Production ◽  
Major Step ◽  
Enzymatic Transesterification ◽  
Microalgal Oil ◽  
Extraction Solvent ◽  
Oil Crops ◽  
Reaction Media

Biodiesel is considered a promising replacement to petroleum-derived diesel. Using oils extracted from agricultural crops competes with their use as food and cannot realistically satisfy the global demand of diesel-fuel requirements. On the other hand, microalgae, which have a much higher oil yield per hectare, compared to oil crops, appear to be a source that has the potential to completely replace fossil diesel. Microalgae oil extraction is a major step in the overall biodiesel production process. Recently, supercritical carbon dioxide (SC-CO2) has been proposed to replace conventional solvent extraction techniques because it is nontoxic, nonhazardous, chemically stable, and inexpensive. It uses environmentally acceptable solvent, which can easily be separated from the products. In addition, the use of SC-CO2 as a reaction media has also been proposed to eliminate the inhibition limitations that encounter biodiesel production reaction using immobilized enzyme as a catalyst. Furthermore, using SC-CO2 allows easy separation of the product. In this paper, conventional biodiesel production with first generation feedstock, using chemical catalysts and solvent-extraction, is compared to new technologies with an emphasis on using microalgae, immobilized lipase, and SC-CO2 as an extraction solvent and reaction media.

scholarly journals Statistical Optimization of Biodiesel Production from Salmon Oil via Enzymatic Transesterification: Investigation of the Effects of Various Operational Parameters

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 700
Author(s):  
Vegneshwaran V. Ramakrishnan ◽  
Deepika Dave ◽  
Yi Liu ◽  
Winny Routray ◽  
Wade Murphy
Keyword(s):  
Atlantic Salmon ◽  
Immobilized Lipase ◽  
Enzyme Concentration ◽  
Biodiesel Production ◽  
Molar Ratio ◽  
Operational Parameters ◽  
Enzymatic Transesterification ◽  
Optimum Parameters ◽  
Response Surface Modelling ◽  
The Relationship

The enzymatic transesterification of Atlantic salmon (Salmo salar) oil was carried out using Novozym 435 (immobilized lipase from Candida antartica) to produce biodiesel. A response surface modelling design was performed to investigate the relationship between biodiesel yield and several critical factors, including enzyme concentration (5, 10, or 15%), temperature (40, 45, or 50 °C), oil/alcohol molar ratio (1:3, 1:4, or 1:5) and time (8, 16, or 24 h). The results indicated that the effects of all the factors were statistically significant at p-values of 0.000 for biodiesel production. The optimum parameters for biodiesel production were determined as 10% enzyme concentration, 45 °C, 16 h, and 1:4 oil/alcohol molar ratio, leading to a biodiesel yield of 87.23%. The step-wise addition of methanol during the enzymatic transesterification further increased the biodiesel yield to 94.5%. This is the first study that focused on Atlantic salmon oil-derived biodiesel production, which creates a paradigm for valorization of Atlantic salmon by-products that would also reduce the consumption and demand of plant oils derived from crops and vegetables.

scholarly journals Production of biodiesel from microalgae

2014 ◽  
Vol 68 (2) ◽  
pp. 213-232 ◽  
Author(s):  
Bojana Danilovic ◽  
Jelena Avramovic ◽  
Jovan Ciric ◽  
Dragisa Savic ◽  
Vlada Veljkovic
Keyword(s):  
Negative Impact ◽  
Growth Conditions ◽  
Production Costs ◽  
Biodiesel Production ◽  
Biofuel Production ◽  
Microalgal Oil ◽  
Oil Crops ◽  
Microalgae Oil ◽  
High Production ◽  
Oil Productivity

In recent years, more attention has been paid to the use of third generation feedstocs for the production of biodiesel. One of the most promising sources of oil for biodiesel production are microalgae. They are unicellular or colonial photosynthetic organisms, with permanently increasing industrial application in the production of not only chemicals and nutritional supplements but also biodiesel. Biodiesel productivity per hectare of cultivation area can be up to 100 times higher for microalgae than for oil crops. Also, microalgae can grow in a variety of environments that are often unsuitable for agricultural purposes. Microalgae oil content varies in different species and can reach up to 77% of dry biomass, while the oil productivity by the phototrophic cultivation of microalgae is up to 122 mg/l/d. Variations of the growth conditions and the implementation of the genetic engineering can induce the changes in the composition and productivity of microalgal oil. Biodiesel from microalgae can be produced in two ways: by transesterification of oil extracted from biomass or by direct transesterification of algal biomass (so called in situ transesterification). This paper reviews the curent status of microalgae used for the production of biodiesel including their isolation, cultivation, harvesting and conversion to biodiesel. Because of high oil productivity, microalgae will play a significant role in future biodiesel production. The advantages of using microalgae as a source for biofuel production are increased efficiency and reduced cost of production. Also, microalgae do not require a lot of space for growing and do not have a negative impact on the global food and water supplies. Disadvantages of using microalgae are more difficult separation of biomass and the need for further research to develop standardized methods for microalgae cultivation and biodiesel production. Currently, microalgae are not yet sustainable option for the commercial production of biodiesel. First of all, the price of biodiesel from microalgae is still higher than the price of diesel due to high production costs.

scholarly journals Enzymatic Transesterification of Waste Frying Oil from Local Restaurants in East Colombia Using a Combined Lipase System

2020 ◽  
Vol 10 (10) ◽  
pp. 3566
Author(s):  
Mary Angélica Ferreira Vela ◽  
Juan C. Acevedo-Páez ◽  
Nestor Urbina-Suárez ◽  
Yeily Adriana Rangel Basto ◽  
Ángel Darío González-Delgado
Keyword(s):  
Reaction Time ◽  
Methyl Esters ◽  
Enzyme Concentration ◽  
Operating Conditions ◽  
Frying Oil ◽  
Biodiesel Production ◽  
Optimum Operating ◽  
Waste Frying Oil ◽  
Production Chain ◽  
Enzymatic Transesterification

The search for innovation and biotechnological strategies in the biodiesel production chain have become a topic of interest for scientific community owing the importance of renewable energy sources. This work aimed to implement an enzymatic transesterification process to obtain biodiesel from waste frying oil (WFO). The transesterification was performed by varying reaction times (8 h, 12 h and 16 h), enzyme concentrations of lipase XX 25 split (14%, 16% and 18%), pH of reaction media (6, 7 and 8) and reaction temperature (35, 38 and 40 °C) with a fixed alcohol–oil molar ratio of 3:1. The optimum operating conditions were selected to quantify the amount of fatty acid methyl esters (FAMEs) generated. The highest biodiesel production was reached with an enzyme concentration of 14%, reaction time of 8 h, pH of 7 and temperature of 38 °C. It was estimated a FAMEs production of 42.86% for the selected experiment; however, best physicochemical characteristics of biodiesel were achieved with an enzyme concentration of 16% and reaction time of 8 h. Results suggested that enzymatic transesterification process was favorable because the amount of methyl esters obtained was similar to the content of fatty acids in the WFO.

Biodiesel production from crude jatropha oil catalyzed by immobilized lipase/acyltransferase from Candida parapsilosis in aqueous medium

2016 ◽  
Vol 218 ◽  
pp. 1224-1229 ◽  
Author(s):  
Joana Rodrigues ◽  
Véronique Perrier ◽  
Jérôme Lecomte ◽  
Eric Dubreucq ◽  
Suzana Ferreira-Dias
Keyword(s):  
Aqueous Medium ◽  
Candida Parapsilosis ◽  
Immobilized Lipase ◽  
Biodiesel Production ◽  
Jatropha Oil

scholarly journals A Robust Two-Step Process for the Efficient Conversion of Acidic Soybean Oil for Biodiesel Production

Catalysts ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 527 ◽  
Author(s):  
Gaojian Ma ◽  
Lingmei Dai ◽  
Dehua Liu ◽  
Wei Du
Keyword(s):  
Fatty Acid ◽  
Soybean Oil ◽  
Lower Cost ◽  
Immobilized Lipase ◽  
Acid Methyl Ester ◽  
Acid Value ◽  
Catalytic Performance ◽  
Raw Material ◽  
Biodiesel Production ◽  
Negative Effect

Acidic oil, which is easily obtained and with lower cost, is a potential raw material for biodiesel production. Apart from containing large quantity of FFAs (free fatty acids), acidic oil usually contains some amount of inorganic acid, glycerides and some other complex components, leading to complicated effect on lipase’s catalytic performance. Exploring the efficient process of converting acidic oil for biodiesel production is of great significance to promote the use of acidic oil. A two-step conversion process for acidic soybean oil was proposed in this paper, where sulfuric acid-mediated hydrolysis was adopted first, then the hydrolyzed free fatty acid, collected from the upper oil layer was further subject to the second-step esterification catalyzed by immobilized lipase Novozym435. Through this novel process, the negative effect caused by harmful impurities and by-product glycerol on lipase was eliminated. A fatty acid methyl ester (FAME) yield of 95% could be obtained with the acid value decreased to 4 mgKOH/g from 188 mgKOH/g. There was no obvious loss in lipase’s activity and a FAME yield of 90% could be maintained with the lipase being repeatedly used for 10 batches. This process was found to have a good applicability to different acidic oils, indicating it has great prospect for converting low quality oil sources for biodiesel preparation.

scholarly journals Sunflower Genotype Selection for Oil Production in the Pre-Amazon Region of Brazil

2019 ◽  
Vol 11 (8) ◽  
pp. 248
Author(s):  
Francirose Shigaki ◽  
Ludhanna Marinho Veras ◽  
Elane Tyara de Jesus Siqueira ◽  
José Roberto Brito Freitas ◽  
Mayanna Karlla Lima Costa ◽  
...  
Keyword(s):  
Experimental Period ◽  
Climatic Conditions ◽  
Amazon Region ◽  
Biodiesel Production ◽  
Alternative Source ◽  
Animal Fats ◽  
Oil Crops ◽  
Energy Needs ◽  
High Production ◽  
Alternative Sources

Part of the energy consumed in the world comes from limited sources, which eventually are expected to be depleted. The search for alternative sources to meet energy needs is crucial. Biodiesel derived from vegetable oils and animal fats stands out as a biodegradable and renewable alternative source of energy. Sunflower is among the top four oil crops produced worldwide, and Brazil has a high production potential for this crop. This study aimed to identify the sunflower genotypes with the highest potential for biodiesel production in the Pre-amazon region of Brazil, where the advance of agricultural frontier represents an important role on biodiesel production. This study was conducted over 2 years of observations. The following genotypes were used: M734 (T), Helio358, EMBRAPA 122, and BRS G 35. The following parameters were assessed: initial flowering date (IFD), physiological maturity date (PMD), plant height (PH), grain yield (YIELD), thousand achene weight (TAW), and oil content (%OC). The climatic conditions of each experimental period were distinctive and directly affected the results obtained. The genotype Embrapa 122 showed the best performance regarding yield and was recommended for the pre-Amazon region of Brazil as the best adapted genotype to the local environmental conditions.

scholarly journals Extraction Solvents Composition Effect In Biodiesel Production From Microalgae Consortium Botryococcus Braunii And Dunaliella Sp.

Khazanah ◽  
2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Tiara Nur Azizah ◽  
◽  
Alya Putri Ramadhanty ◽  
Nadya Feranika ◽  
◽  
...  
Keyword(s):  
Fossil Fuels ◽  
Production Capacity ◽  
Water Concentration ◽  
Soxhlet Extraction ◽  
Botryococcus Braunii ◽  
Solvent Mixture ◽  
Biodiesel Production ◽  
Solvent Ratio ◽  
Extraction Solvent ◽  
Biodiesel Synthesis

Indonesia has entered an energy emergency phase, proven that Indonesia is no longer a surplus oil producer due to the productivity of the wells decrease over the years and the pattern of people's consumption of fossil fuels has been exceeding the production capacity. Therefore, we need the right solution to overcome this problem, which is developing biodiesel as renewable energy based on microalgae oil. The microalgae used in this research is the consortium of microalgae Botryococcus braunii and Dunaliella sp. Microalgae were cultivated and harvested through the dewatering process with 1 g naoh/1 L water concentration. Water contents of cultivated Botryococcus braunii are 60.2505% while Dunaliella sp. Is 64.5002%. The oil from microalgae is obtained by extracted dry microalgae through the soxhlet extraction (leaching) method with mixed solvent n-hexane and ethanol as the co-solvent using a variety of solvent ratio 2:1 and 3:1. Pure microalgae consortium oil separated from the solvent using the distillation process then analyzed with GC-FID. The analysis result is trans-linoleic acid is the most dominant fatty acid contained in this oil. Transesterification process with cao (1.5% of oil weight) as a catalyst. The results obtained from this study are the oil yield 72% extracted with a solvent mixture of n-hexane and ethanol 2:1 and 60.4% for 3:1. The biodiesel synthesis resulted in the amount of yield obtained from the solvent ratio 2:1 extraction is 94.3%, while with solvent ratio 3:1 is 79.2%. The quality of both biodiesels has met the requirements of SNI 7182:2015 and ASTM D7467, except the density of biodiesel with extraction solvent composition 3:1 which is below the standard. Therefore, the best biodiesel quality is obtained from microalgae consortium’s oil with the composition of extraction solvent 2:1.

scholarly journals The anthocyanin pigment extract from red rose as antibacterial agent

2018 ◽  
Vol 14 (1-2) ◽  
pp. 184-187
Author(s):  
Elfi Anis Saati ◽  
Annisa Dyah Pusparini ◽  
Mochammad Wachid ◽  
Sri Winarsih
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Solvent Extraction ◽  
Shelf Life ◽  
Antibacterial Agent ◽  
Block Design ◽  
Pseudomonas Sp ◽  
Anthocyanin Pigment ◽  
Extraction Solvent ◽  
Pigment Extract

Red rose consisting of anthocyanin pigment has been used as an antibacterial agent. However, there is no study on the anthocyanin pigment extract from red rose as the antibacterial agent. The effectiveness of the antibacterial agent can be affected by the solvent extraction and the flower shelf life. Here, we report the effects of solvent extraction and red rose (Rosa sp.) shelf life on the antibacterial activity. Red rose concentrated extraction and randomized complete block design factorial was carefully used with factors of long display and solvent extraction. The extraction solvent (P) comprised of water, ethanol, and mixture of water-ethanol (1 : 1), while the red rose shelf life (M) consisted of 0, 2, 4 and 6 days. Moreover, pH, antioxidant activity and minimum bactericidal concentration (MBC) with four variations of concentrated concentration (100%, 50%, 25% and 12.5%) on Escherichia coli, Salmonella thypi, and Pseudomonas sp. were analyzed. After two days of shelf life using water as the solvent for extraction, the antioxidant activity achieved 79% at pH of 2.5. It was also demonstrated that it was able to kill all the investigated bacterias, which were Escherichia coli, Salmonella thypi, and Pseudomonas sp. with concentrated concentrations of 100%, 50% and 25%. When the concentrated concentration was 12.5%, the MBC value was constantly found to be 1.39 × 108 cfu/g for Escherichia coli and 9.53 × 107 cfu/g for Salmonella thypi.

scholarly journals Potensi Pengembangan Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw)di Lahan Terdegradasi

Perspektif ◽  
2016 ◽  
Vol 14 (2) ◽  
pp. 87 ◽  
Author(s):  
DIBYO PRANOWO ◽  
MAMAN HERMAN ◽  
. SYAFARUDDIN
Keyword(s):  
Renewable Energy ◽  
Economic Value ◽  
Raw Material ◽  
Biodiesel Production ◽  
Degraded Land ◽  
Marginal Lands ◽  
Productivity Level ◽  
Oil Crops ◽  
Energy Needs ◽  
Alternative Sources

<p>ABSTRAK<br /><br />Kemiri sunan (Reutealis trisperma (Blanco) Airy Shaw) merupakan salah satu jenis tanaman penghasil minyak nabati yang memiliki potensi besar sebagai sumber bahan baku untuk biodiesel. Tingkat produktivitas yang dapat mencapai 8-9 ton minyak kasar atau setara dengan 6-8 ton biodiesel/ha/tahun memiliki nilai strategis terkait dengan program pemerintah dalam mencari alternatif sumber energi baru yang terbarukan. Pengembangan sumber energi terbarukan seperti yang berasal dari minyak nabati kemiri sunan merupakan salah satu alternatif dalam upaya memenuhi defisit energi untuk keperluan domestik sehingga Indonesia dapat keluar dari himpitan krisis energi. Lahan-lahan yang telah terdegradasi di Indonesia dari tahun ke tahun luasnya semakin bertambah baik karena faktor alam maupun karena eksploitasi yang tidak terkendali. Disisi lain pengembangan tanaman sumber BBN terkendala karena keterbatasan lahan. Kajian yang telah dilakukan secara intensif terhadap karakteristik tanaman, minyak dan biodiesel yang dihasilkannya, serta daya adaptasinya yang sangat luas terhadap beragam agroekosistem yang ada di Indonesia, tanaman kemiri sunan memberikan harapan yang baik disamping sebagai sumber bahan baku biodiesel, juga dapat berfungsi sebagai tanaman konservasi untuk mereklamasi lahan-lahan marginal yang telah terdegradasi. Disamping itu, pengembangan tanaman kemiri sunan di lahan yang telah terdegradasi tidak hanya akan dapat meningkatkan nilai ekonomi lahan tersebut, tetapi juga dapat dijadikan tanaman yang bernilai ekonomi tinggi, serta mampu menyediakan kebutuhan energi bagi masyarakat sekitar maupun ke wilayah yang lebih luas. <br />Kata kunci: Kemiri sunan, biodiesel, energi baru terbarukan, lahan terdegradasi, lahan bekas tambang.<br /><br />ABSTRACT</p><p>The Multiple Benefits of Developing Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) In Degraded Land<br /><br />Kemiri sunan (Reutealis trisperma (Blanco) Airy Shaw) is one kind of vegetable oil crops that have great potential as a source of raw material for biodiesel. The productivity level that can reach 8-9 tons of crude oil, equivalent to 6-8 tons of biodiesel/ha/year make as a strategic commodity associated with government programs to find alternative sources of renewable energy. Development of renewable energy such as from vegetable oils of kemiri sunan is one of the alternatives in an effort to solve the deficit of energy for domestic use so that Indonesia can way out of the crush of the energy crisis. Lands that have been degraded in Indonesia continuously increasing both cause of the extent of natural factors and uncontrolled exploitation. On the other hand the development of this plants retricted by aviability of land. The research88 Volume 14 Nomor 2, Des 2015 : 87 - 101 studies have been conducted on the characteristics of plants, oil and biodiesel production, and adaptability in very broadly of Indonesian agro-ecosystem, this plant show well hopes besides as a source of raw material for biodiesel, it can also function as a conservation plant to reclaim marginal lands that have been degraded. In addition, the development of kemiri sunan on degraded land will not only be able to increase the economic value of the land, but also can be used as crops of high economic value, and able to provide for the energy needs of the surrounding communities and to the wider region.<br />Keywords: Reutealis trisperma (Blanco) Airy Shaw, biodiesel, renewable energy, degraded land, post mained land.</p>

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