scholarly journals Reliability analysis of two methods for measuring active enamel demineralization: An in vitro study

2013 ◽  
Vol 07 (02) ◽  
pp. 159-164 ◽  
Author(s):  
Camila Nassur ◽  
Luciana Pomarico ◽  
Lucianne Cople Maia
Keyword(s):  
Image Analysis ◽  
Intraclass Correlation ◽  
In Vitro Study ◽  
Analysis Software ◽  
Computerized Image Analysis ◽  
Enamel Demineralization ◽  
Significant Difference ◽  
Digital Caliper ◽  
Excellent Reproducibility

ABSTRACT Objective: The aim was to evaluate the intra- and inter-examiner reliability of two methods, namely, digital caliper [DC] and computerized image analysis software (Image Tool [IT] version 4.1) for measuring the area of an active enamel demineralization lesion. The effect on that measurement of a window that delimits the lesion was also assessed. Materials and Methods: Thirty-eight circular artificial enamel demineralization lesions (r = 2 mm) were produced on bovine teeth in vitro, which were then measured three times by three examiners, with and without the presence of a window delimiting the area to be measured. The data were analyzed by the intraclass correlation coefficient (ICC) with 95% confidence interval (CI) and by the Altman and Bland analysis. Results: The intra-examiner correlation using the IT method showed excellent reproducibility (mean ICC values 0.922-0.970 with the delimiting window and 0.915-0.990 without the window). However, the intra-examiner correlation using the DC method showed comparatively less reproducibility in the measurements (mean ICC values 0.458-0.648 with the delimiting window and 0.378-0.665 without the window). The inter-examiner correlations showed very good reproducibility of the measurements regardless of the presence or absence of the window for both the DC method (0.811-0.846) and IT method (0.953-0.994). However, the latter method showed less variability within the measurements. Conclusion: However, statistically no significant difference was found between both methodologies. Nevertheless, computerized image analysis with the Image Tool software demonstrated higher intra- and inter-examiner reliability than the digital caliper method, in estimating the area of the enamel demineralization lesion, regardless of the delimiting window.

scholarly journals Effect of Different Software Programs on the Accuracy of Dental Scanner Using Three-Dimensional Analysis

2021 ◽  
Vol 18 (16) ◽  
pp. 8449
Author(s):  
Keunbada Son ◽  
Wan-Sun Lee ◽  
Kyu-Bok Lee
Keyword(s):  
Reference Model ◽  
Three Dimensional ◽  
In Vitro Study ◽  
3D Analysis ◽  
Analysis Software ◽  
Test Models ◽  
Significant Difference ◽  
Tooth Preparation ◽  
Software Programs

This in vitro study aimed to evaluate the 3D analysis for complete arch, half arch, and tooth preparation region by using four analysis software programs. The CAD reference model (CRM; N = 1 per region) and CAD test models (CTMs; N = 20 per software) of complete arch, half arch, and tooth preparation were obtained by using scanners. For both CRM and CTMs, mesh data other than the same area were deleted. For 3D analysis, four analysis software programs (Geomagic control X, GOM Inspect, Cloudcompare, and Materialise 3-matic) were used in the alignment of CRM and CTMs as well as in the 3D comparison. Root mean square (RMS) was regarded as the result of the 3D comparison. One-way analysis of variance and Tukey honestly significant difference tests were performed for statistical comparison of four analysis software programs (α = 0.05). In half-arch and tooth preparation region, the four analysis software programs showed a significant difference in RMS values (p < 0.001), but in complete-arch region, no significant difference was found among the four software programs (p = 0.139). As the area of the virtual cast for 3D analysis becomes smaller, variable results are obtained depending on the software program used, and the difference in results among software programs are not considered in the 3D analysis for complete-arch region.

scholarly journals Studying the Optical 3D Accuracy of Intraoral Scans: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Pokpong Amornvit ◽  
Sasiwimol Sanohkan ◽  
Chaimongkon Peampring
Keyword(s):  
High Resolution ◽  
Computer Software ◽  
In Vitro Study ◽  
Reference Points ◽  
Washington Dc ◽  
Scanning Area ◽  
Significant Difference ◽  
Post Hoc ◽  
Digital Caliper

There are various scanners available in dental practice with various accuracies. The aim of this study was to compare the 3D capturing accuracy of scans obtained from Trios 3 and Dental Wings scanner. A reference mandibular model was printed from FormLab with reference points in three axes (X, Y, and XY and Z). The printed model was scanned 5 times with 3 scans: normal scan by Trios 3 (Trios 3A), high-resolution scan by Trios 3 (Trios 3B), and normal scan by Dental Wings. After scan, the stereolithography (stl) files were generated. Then, the measurements were made from the computer software using Rhinoceros 3D (Rhino, Robert McNeel & Associates for Windows, Washington DC, USA). The measurements made with digital caliper were taken as control. Statistical analysis was done using one-way ANOVA with post hoc using Sheffe (P<0.01). Trios 3 presented higher accuracy than Dental Wings and high resolution showed better results. The Dental Wings showed less accuracy at the measurements >50 mm of length and >30 mm in width. There was no significant difference (P>0.05) of control with the Trios 3A and Trios 3B. Similarly, for the measurements in Z-axis, there was no significant difference of control with each scan (Trios 3A, Trios 3B, and Dental Wings). Accuracy of the scan is affected by the length of the scanning area and scanning pattern. It is less recommended to Dental Wings scan >3-unit prosthesis and that crosses the midline.

scholarly journals Evaluation of High-Speed Handpiece Cutting Efficiency According to Bur Eccentricity: An In Vitro Study

2019 ◽  
Vol 9 (16) ◽  
pp. 3395
Author(s):  
Duk-Yeon Kim ◽  
Keunbada Son ◽  
Kyu-bok Lee
Keyword(s):  
High Speed ◽  
Reference Model ◽  
In Vitro Study ◽  
Lithium Disilicate ◽  
3D Analysis ◽  
Analysis Software ◽  
Cutting Efficiency ◽  
Significant Difference ◽  
Cutting Model

This study aimed to evaluate the correlation between the cutting efficiency and bur eccentricity of high-speed handpieces. The prepared lithium disilicate samples were digitized using a 3D model scanner (reference model, RM) (n = 45), and the lithium disilicate samples were cut using three high-speed handpieces. To evaluate the cutting efficiency, the cut lithium disilicate sample was digitized (cutting model, CM), and the RM and CM were superimposed using a 3D analysis software. Bur eccentricity of the high-speed handpieces was measured using dedicated equipment. Statistical analyses were performed using an analysis software. The statistical differences in pairwise comparisons (α = 0.05) were analyzed using the Kruskal–Wallis and post hoc tests. The S-max M600 obtained a cutting efficiency of 6.13 mm3. TG-98 and TRAUS ATN-400 showed similar efficiencies of 2.914 and 3.05 mm3, respectively. There was a significant difference in the cutting efficiency of the S-max M600 compared with TG-98 and TRAUS ATN-400 (p < 0.001). S-max M600 had an eccentricity of 3.507 µm. TG-98 and TRAUS ATN-400 had eccentricities of 5.99 and 7.767 µm, respectively. There were statistically significant differences in the eccentricity among all the high-speed handpieces (p < 0.001).

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals Marginal and Internal Fit of Ceramic Prostheses Fabricated from Different Chairside CAD/CAM Systems: An In Vitro Study

2021 ◽  
Vol 11 (2) ◽  
pp. 857
Author(s):  
Keunbada Son ◽  
Kyu-Bok Lee
Keyword(s):  
Computer Aided Design ◽  
In Vitro Study ◽  
Vitro Study ◽  
Marginal Gap ◽  
Significant Difference ◽  
Cad Cam ◽  
Ceramic Crowns ◽  
Internal Fit ◽  
Cam Systems

The purpose of this in vitro study was to evaluate marginal and internal fits of ceramic crowns fabricated with chairside computer-aided design and manufacturing (CAD/CAM) systems. An experimental model based on ISO 12836:2015 was digitally scanned with different intraoral scanners (Omnicam (CEREC), EZIS PO (DDS), and CS3500 (Carestream)). Ceramic crowns were fabricated using the CAD/CAM process recommended by each system (CEREC, EZIS, and Carestream systems; N = 15). The 3-dimensional (3D) marginal and internal fit of each ceramic crown was measured using a 3D inspection software (Geomagic control X). Differences among the systems and various measurements were evaluated using the Kruskal–Wallis test. Statistically significant differences were validated using pairwise comparisons (α = 0.05). Occlusal gaps in the CEREC, EZIS, and Carestream groups were 113.0, 161.3, and 438.2 µm, respectively (p < 0.001). The axial gaps were 83.4, 78.0, and 107.9 µm, respectively. The marginal gaps were 77.8, 99.3, and 60.6 µm, respectively, and the whole gaps were 85.9, 107.3, and 214.0 µm, respectively. Significant differences were observed with the EZIS system compared with the other two systems in terms of the marginal gap sizes. The CEREC system showed no significant differences among the four measured regions. However, the EZIS and Carestream systems did show a statistically significant difference (p < 0.05). All three systems were judged to be capable of fabricating clinically acceptable prostheses, because the marginal gap, which is the most important factor in the marginal fit of prostheses, was recorded to be below 100 µm in all three systems.

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P &lt; .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

A novel intracranial exchange guidewire improves the navigation of various endovascular devices: An in vitro study of challenging situations

2021 ◽  
pp. 159101992110573
Author(s):  
Naoki Kaneko ◽  
Ariel Takayanagi ◽  
Hamidreza Saber ◽  
Lea Guo ◽  
Satoshi Tateshima
Keyword(s):  
Giant Aneurysm ◽  
In Vitro Study ◽  
Target Vessel ◽  
Success Rates ◽  
Intracranial Stenting ◽  
Aneurysm Neck ◽  
Significant Difference ◽  
Aneurysm Model ◽  
Endovascular Devices

Objective Neuroendovascular procedures rely on successful navigation and stable access to the target vessel. The Stabilizer is a 300 cm long exchange wire with a 0.014 diameter and a soft, flexible stent at the distal end designed to assist with navigation and device delivery. This study aims to assess the efficacy of the Stabilizer for navigation in a variety of challenging environments. Methods The efficacy of the Stabilizer was evaluated using three challenging vascular models: a giant aneurysm model, a severe tortuosity model, and an M1 stenosis model. The Stabilizer was compared with a conventional wire during navigation in each model. Results In the giant aneurysm model, there was no significant difference of success during straightening of a looped wire and significantly higher success rates when advancing an intermediate catheter with the Stabilizer beyond the aneurysm neck compared to a conventional guidewire. The Stabilizer also significantly increased success rates when advancing an intermediate catheter through a model with severe tortuosity compared to a conventional guidewire, as well as exchange maneuver for intracranial stenting in a stenosis model compared to an exchange wire. Conclusions In our experimental model, the Stabilizer significantly improved navigation and device delivery in a variety of challenging settings compared to conventional wires.

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals The Accuracy and Reliability of Tooth Shade Selection Using Different Instrumental Techniques: An In Vitro Study

Sensors ◽  
2021 ◽  
Vol 21 (22) ◽  
pp. 7490
Author(s):  
Nattapong Sirintawat ◽  
Tanyaporn Leelaratrungruang ◽  
Pongsakorn Poovarodom ◽  
Sirichai Kiattavorncharoen ◽  
Parinya Amornsettachai
Keyword(s):  
Intraclass Correlation ◽  
In Vitro Study ◽  
Control Group ◽  
Intraoral Scanner ◽  
Control Groups ◽  
B Values ◽  
Cie Lab ◽  
And Control ◽  
Shade Selection

This study aimed to investigate and compare the reliability and accuracy of tooth shade selection in the model using 30 milled crowns via five methods: (1) digital single-lens reflex (DSLR) camera with twin flash (TF) and polarized filter (DSLR + TF), (2) DSLR camera with a ring flash (RF) and polarized filter (DSLR + RF), (3) smartphone camera with light corrector and polarized filter (SMART), (4) intraoral scanner (IOS), and (5) spectrophotometer (SPEC). These methods were compared with the control group or manufacturer’s shade. The CIE Lab values (L, a, and b values) were obtained from five of the methods to indicate the color of the tooth. Adobe Photoshop was used to generate CIE Lab values from the digital photographs. The reliability was calculated from the intraclass correlation based on two repetitions. The accuracy was calculated from; (a) ΔE calculated by the formula comparing each method to the control group, (b) study and control groups were analyzed by using the Kruskal–Wallis test, and (c) the relationship between study and control groups were calculated using Spearman’s correlation. The reliability of the intraclass correlation of L, a, and b values obtained from the five methods showed satisfactory correlations ranging from 0.732–0.996, 0.887–0.994, and 0.884–0.999, respectively. The ΔE from all groups had statistically significant differences when compared to the border of clinical acceptance (ΔE = 6.8). The ΔE from DSLR + TF, DSLR + RF, SMART, and SPEC were higher than clinical acceptance (ΔE > 6.8), whereas the ΔE from IOS was 5.96 and all of the L, a, and b values were not statistically significantly different from the manufacturer’s shade (p < 0.01). The ΔE of the DSLR + RF group showed the least accuracy (ΔE = 19.98), whereas the ∆E of DSLR + TF, SMART, and SPEC showed similar accuracy ∆E (ΔE = 10.90, 10.57, and 11.57, respectively). The DSLR camera combined with a ring flash system and polarized filter provided the least accuracy. The intraoral scanner provided the highest accuracy. However, tooth shade selection deserves the combination of various techniques and a professional learning curve to establish the most accurate outcome.

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