scholarly journals Immunomodulatory Effect of Moringa oleifera Lam. Aqueous Extract on the Burrowing Crab, Cardiosoma guanhumi (Latreille, 1828)

2021 ◽  
Vol 41 (3) ◽  
Author(s):  
I.F Jesuniyi ◽  
R.O Moruf ◽  
A.O Lawal-Are

Moringa oleifera has impressive range of nutritional and medicinal values which when consumed have influence on hematological profile which is applied as an index of physiological condition of various organisms and thus provide information about the health status of local populations. The study aimed to determine the effects of M. oleifera aqueous leaves extract on the hematology, serum biochemical profile and antioxidant enzyme activities of the burrowing crab, Cardiosoma guanhumi. Thirty juveniles of C. guanhumi (Average weight 43.20±0.05 g) were tested, where six crabs were randomly selected and distributed per each tank (1 x 1x 0.6m3) of 1litre of water. Different diets containing M. oleifera aqueous extract at inclusion levels of 0.5 ml, 1.0 ml, 1.5 ml, 2.0 ml and control were used to feed the crabs. Total Haemocyte Count ranged between 2733.33±0.90 mL and 6350.00±0.60 mL; there were significant differences among the treatment groups (p < 0.05). Haemocyte sub-population variables showed that crabs fed the control and 2.0ml M. oleifera kg-1 diet had increased in the granulocyte and monocyte populations but a decrease in the agranulocytes. The results of the serum enzymes showed an increase as the level of M. oleifera aqueous extract increases in the diet. Highest superoxide dismutase (75.43 ±21.25 min/mg pro) and catalase activities (2.96±0.18 min/mg pro), malondialdehyde (12.05±2.09 nmol/L) and glutathione concentrations (0.19 ±0.02 μmol/L) were recorded in crabs fed diet T4 (2.0ml M. oleifera kg-1), while the lowest were obtained in crabs fed control diet. The present study showed that the inclusion of M. oleifera aqueous extract up to 1.0ml kg-1 will have immunomodulatory performance on Cardiosoma guanhumi without any deleterious effect on the crab’s health status. Keywords: Hemato-Biochemicals, Land Crab, Lagos Lagoon, Plant Extract, Nigeria

2021 ◽  
Vol 8 ◽  
Author(s):  
Lily P. Hernandez ◽  
James L. Dunn ◽  
Joel Wenninghoff ◽  
Amanda Hesse ◽  
Crystal L. Levesque

Times of high metabolic activity in gestation and lactation, as well as periods of stress at weaning, can lead to greater incidences of oxidative stress in the dam and offspring during the suckling and postweaning period. Oxidative stress is an imbalance between prooxidant molecules and the antioxidant defense system that can negatively impact growth and/or reproductive performance. The objective of this research was to evaluate the effectiveness of whole yeast cell, peppermint oil, and ɤ-tocopherol in gestation and lactation on maternal oxidative status and offspring growth from birth to market. In study 1, 45 sows and gilts were assigned to one of four diets [control diet (CON), control + whole yeast cell (YC), control + mint oil top dress (MO), and control + yeast cell and mint oil top dress (YCMO)] provided from d110 of gestation through to weaning. A total of 481 weaned offspring were randomly allotted to pens balanced by weight and litter within maternal treatment and received the same dietary treatment as the sow for 35 days postwean in a four-phase feeding regimen. In study 2, 53 sows and gilts were allotted to four diet regimens similar to study 1 [CON, YC, MO, and control + ɤ-tocopherol (GT)] from d5 postbreeding to weaning. At weaning, 605 piglets were randomly allotted to pens, balanced by weight and litter within maternal treatment and fed a common diet for 126 days postwean in a nine-phase feeding regimen. Maternal dietary treatment did not impact sow body weight, piglet birth weight, and litter size in either study. In study 1, piglets from YC sows were heavier (p &lt; 0.05) at weaning than CON animals. In the postwean period, overall daily gain was greater (p &lt; 0.05) for CON-fed pigs than YCMO pigs, with overall feed intake greater (p &lt; 0.05) for YCMO- than MO-fed pigs, resulting in lower (p &lt; 0.05) Gain to Feed (G:F) in YCMO-fed pigs. In study 1, glutathione content in milk tended to be lower (p &lt; 0.10) in MO than in YCMO sows. In study 2, piglets from GT-fed sows tended to be heavier (p &lt; 0.10) at weaning than YC piglets. Lightweight pigs from CON sows tended to be lighter (p &lt; 0.10) than pigs from all other treatment groups at weaning and day (d) 29 postwean. Lightweight MO and GT pigs were heavier at d42 (p &lt; 0.05) than CON and YC pigs. At d70 postwean, GT pigs tended to be heavier than CON pigs. Lightweight MO pigs had greater gain (p &lt; 0.05) during the finishing period than all other treatment groups. With respect to sow oxidative status in study 2, glutathione content in colostrum and d4 and 14 milk samples did not differ by maternal treatment. Superoxide dismutase activity in sow sera, colostrum, and milk did not differ between diets in either study. Whole yeast cell and ɤ-tocopherol supplementation in sow lactation diets resulted in heavier offspring. However, pre- and postnatal exposure to mint oil benefited lightweight pigs up to market weight.


2020 ◽  
Vol 40 (1) ◽  
pp. 79-82
Author(s):  
C. T. Ezeokeke

Experiment was carried out to determine performance of day old local fowls, fed supplemented plantain peels meal (PPM). The birds were arranged in completely randomized design having six birds per replicate and two treatments comprising one test diet and one control diet. At starter phase, all the parameters (body weight, body weight gain, feed intake and feed conversion ratio) measured were not significant (p>0.05). The birds on the test diet had average initial weight, 42.00g while that of the control had average weight, 43.75g and at 5 weeks of age average weights of 133.30g and 134.54g (p>0.05) ,respectively. At the finisher phase, all the parameters measured were not significant (p>0.5). The prices per kg of feed for the test and control diets were N93.05 and N95.05, respectively. Therefore, PPM inclusion fed at early stage of life of the birds enhanced performance at a lower cost.


Author(s):  
DIAN RATIH LAKSMITAWATI ◽  
UMI MARWATI ◽  
YATI SUMIYATI ◽  
DIAH KARTIKA PRATAMI ◽  
INTAN PERMATA SARI

Objective: This study aims to determine the effect of Amorphophallus muelleri Blume and Moringa oleifera L leaf on body weight, food intake, and hepatic histopathology in mice. Methods: The mice were divided into five groups according to their diet, which includes porang, wheat, porang-moringa, wheat-moringa, and control diet. Each group consists of 5 males and 5 females, which were fed for 28 d, and then analyzed for their body weight, total food intake, alanine aminotransferase (ALT) as well as aspartate aminotransferase (AST) levels in plasma, and hepatic histopathology. Results: The result showed that the group of porang and porang-moringa has lower body weight and feed intake, which is significantly different compared to the others. Furthermore, an increase was observed on plasma AST/ALT activities in 30% porang and 20% porang-moringa group. Also, one of the mice of porang group has inflammatory cell infiltration (++) on histopathology results. Conclusion: It was therefore concluded that feeding containing porang causes low food consumption. Furthermore, weight loss increases AST/ALT and leukocyte infiltration even though a mouse consistently deteriorates.


The main objective of the current study was to investigate the effect of Moringa oleifera leaves and seeds aqueous extracts on the physicochemical properties, microbiological quality and biogenic amines content of semi-dry fermented sausage during ripening process and storage at 4ºC for 3 months. Semi-dry fermented sausages were formulated by using M. oleifera leaves and seeds aqueous extracts at a rate of 1.5% in comparison to control. Incorporation of M. oleifera leaves aqueous extracts during the formulation of fermented sausage resulted in a significant (P<0.05) decrease in pH, lipid oxidation and total volatile nitrogen content while significant (P<0.05) increase in the lactic acid bacteria when compared with those formulated with seeds and control groups. However, the yeast, mold and biogenic amines content of sausages formulated with M. oleifera seeds aqueous extract were significantly (P<0.05) lower than those formulated with leaves and the control. From this study, we can conclude that M. oleifera leaves aqueous extract exhibited potent antioxidant activity while that of seeds exhibited potent antimicrobial activity. Therefore, both M. oleifera extracts can be used as natural additives to improve the quality and safety of semi-dry fermented sausage.


1970 ◽  
Vol 50 (2) ◽  
pp. 311-318 ◽  
Author(s):  
L. C. SEIER ◽  
R. J. KIRK ◽  
T. J. DEVLIN ◽  
R. J. PARKER

Forty-five male Sapphire mink kits were allotted to three treatment groups and fed for approximately 100 days (August 1–November 7). The control diet (treatment 1) consisted of horse-meat 10%, cooked chicken by-products 15%, raw cereal 25%, and whole frozen fish 50%. The frozen fish was replaced by either herring meal or soybean meal on an equivalent dry matter basis for treatments 2 and 3. Average mink weights on November 7 were 1.78, 1.87, and 1.92 kg for the soybean meal, herring meal, and control treatment groups, respectively, and were not significantly different (P > 0.05). The feeding period was divided into a growing phase (period 1), a growing and furring phase (period 2), and a maintenance and a priming phase (period 3). The average daily weight gains during these periods were: period 1: 15.2, 18.0 and 11.8 g; period 2: 6.1, 6.7 and 7.8 g; period 3: +0.8, −2.6 and −0.7 g, for the control, herring- and soybean meal-fed mink, respectively. Digestibility trials were carried out during each period. Dry matter and energy digestibilities were significantly higher (P < 0.05) for the herring-fed and control kits than for the soybean meal-fed kits. There was no significant difference (P > 0.05) in nitrogen balance among treatments. Skin biopsies were taken at pelting time to compare fur density, with values of 19.6, 20.6, and 19.5 hairs per pore, for the soybean-, herring-fed and control kits, respectively.


2016 ◽  
Vol 15 (1) ◽  
pp. 60-68
Author(s):  
O. O ONI ◽  
O. M.O, IDOWU ◽  
J. A. OLANITE ◽  
A. M. BAMGBOSE

A study was conducted to investigate the response of weaner rabbits to graded dietary replacement of wheat offal with dried citrus pulp (DCP) at 0, 10, 20 and 30% (weight/weight) respectively .A total of Sixty-four, male and female weaner rabbits of 56 days old, were randomly assigned to four dietary treatments with sixteen rabbits each and consisting of four rabbits per replicate in a completely ran- domized design. The study lasted for 112 days during which data was obtained on nutrient intake, performance characteristics and carcass yield. The crude protein intake of the rabbits decreased (P<0.05) significantly as the DCP inclusion in the diets increased. There were significant (P<0.01) differences in the values obtained for the final weight, total weight gain, average weight gain, meta- bolic weight gain and growth rate across the treatment groups. The growth rate was 10.29 g/d in the control fed rabbits but further decreased with increasing level of DCP in the diets with values of 11.29, 6.30 and 5.80g/d obtained respectively for rabbit fed with 10%, 20%, and 30% DCP inclusion level. The lowest feed conversion ratio was obtained at the 0% level of DCP inclusion. The retail cut parts indicated a significant (P<0.05) difference in the values for rack and ranged from 11.92 to 18.06g. It was concluded that although, rabbits fed with control diet had the highest overall performance, 10% dietary inclusion of DCP supported improved growth and better carcass yield.


2017 ◽  
Vol 11 (4) ◽  
pp. 289
Author(s):  
Fitra Wahyuni ◽  
Sony Heru Sumarsono

<p align="center"><strong><em>ABSTRACT</em></strong></p><p><em>This study was performed to observe the effect of linamarin on reproductive performance, that consist of the number of live fetuses, reabsorption embryo or fetal, fetal death and fetal body weight together with length. Pregnant mice were divided into one control group with administration of distilled water (0 mg/kgb.w/days) and six treatment groups linamarin dose is 0.36; 0.72; 1.26 mg; 2; 4; and 8 mg/kgb.w./days. Linamarin dosing and control begins at 5<sup>th</sup> to 16<sup>th</sup> gestation days, at 17-days of gestation caesarean section were performed to pregnant mice to remove foetuses. Statistical analysis used was a variation analysis by ANOVA followed by comparisons between treatments HSD test (Tukey). Giving linamarin observed in the parent mice led to a decrease in average weight gain of the mother during pregnancy compared with controls. In linamarin dose 8 mg/kgb.w/day an increase in embryo reabsorption and fetal death, as well as the observed decrease in fetal body weight and length, but after statistical analysis of the decrease was not significantly different. Linamarin with the doses given in this study is not expected to interfere with pregnancy based on reproductive performance were observed.</em></p><p><em> </em></p><p><strong><em>Keywords:</em></strong><em> Linamarin, reproductive performance, fetal weight, fetal body length</em></p><p><em> </em></p><p align="center"><strong><em>ABSTRAK</em></strong></p><p><em>Penelitian ini dilaksanakan bertujuan untuk mengamati pengaruh linamarin pada penampilan reproduksi yang meliputi jumlah fetus hidup, embrio resorpsi, fetus mati dan berat beserta panjang badan fetus. Induk mencit yang bunting dikelompokkan ke dalam satu kelompok kontrol dengan pemberian akuades (0 mg/kgb.b./hari) dan enam kelompok perlakuan dosis linamarin yaitu 0,36; 0,72; 1,26 mg; 2; 4; dan 8 mg/kgb.b./hari. Pemberian dosis linamarin dan kontrol dimulai pada umur kebuntingan 5 sampai 16 hari, setelah itu pada umur kebuntingan 17 hari mencit dikorbankan nyawanya untuk pengambilan fetus. Analisis statistik yang digunakan adalah analisis variasi dengan ANOVA dan dilanjutkan dengan uji perbandingan antar perlakuan HSD (Tukey). Pemberian linamarin pada induk mencit diamati menyebabkan penurunan rata-rata pertambahan berat badan induk selama kebuntingan dibandingkan dengan kontrol. Pada dosis linamarin 8 mg/kgb.b./hari terjadi peningkatan embrio resorpsi dan fetus yang mati, serta diamati penurunan berat dan panjang badan fetus, namun setelah dilakukan analisis statistik penurunan tersebut tidak berbeda nyata. Linamarin dengan dosis yang diberikan pada penelitian ini diduga tidak mengganggu kebuntingan berdasarkan penampilan reproduksi yang diamati.</em></p><p><em> </em></p><p><strong><em>Kata kunci:</em></strong><em> Linamarin, penampilan reproduksi, berat badan fetus, panjang badan fetus</em></p>


Author(s):  
D.E. Beighle ◽  
P.A. Boyazoglu ◽  
R.W. Hemken

ABSTRACT Fifteen Friesian oxen between 12 and 18 months of age with a mean body mass of 240.7 kg were randomly assigned to diets containing 0.25 % phosphorus (P) or less, to evaluate the acute effects of an acidiogenic diet of -11.1 meq/100 g of diet dry matter, compared with a basiogenic diet of +25.6 meq/100 g or a control diet of +16.5 meq/100 g of diet dry matter calculated as (Na + K) - (Cl + S), on blood, bone and faecal P, calcium (Ca) and magnesium (Mg) for a period of 9 weeks. Blood, bone and faecal responses to an anionic diet are described. An inverse relationship existed between bone and blood Ca, in which there was resorption from bone with increased blood Ca in response to the anionic diet. The anionic treatment group demonstrated simultaneous increases in bone, blood and faecal P concentrations at various stages of the experiment compared to the cationic and control treatment groups. Results indicate independent absorption and resorption of Ca and P into and out of bone. There was wide variation in the bone Ca:P ratio between 2.02 and 1.51 among animals fed the anionic diet, with the Ca:P ratio following Ca values and not bone P values. Bone and blood P had a linear relationship with dietary cation:anion balance (DCAB), increasing as the diet became more anionic in nature, but faecal P was curvilinear with highest concentrations at -11.1 and +25.6 meq/100 g compared to +16.5 meq/100 g. Concurrent blood, bone and faecal P increases at some stages of the experiment indicate a P-sparing effect of the anionic diet and warrants further research into the long-term effects of anions in the diet, leading to their use as a possible addition to improved licks in P-deficient areas.


2020 ◽  
Vol 158 (4) ◽  
pp. 339-348
Author(s):  
Tamer M. M. Hassan ◽  
Omar A. Ahmed-Farid ◽  
Fathy A. I. Abdel-Fattah

AbstractPomegranate peels (PP) and mango leaves (ML) were analysed for nutrients and tannin contents. In an in vitro test, ten diets were prepared; six contained 2, 4 and 6% of PP or ML, three diets supplemented with mixed levels of PP and ML (1 + 1%, 2 + 2% and 3 + 3%) and control diet free of them. Gas was measured after 3, 6, 12, 24, 48 and 72 h of incubation. Methane and rumen parameters were estimated. In an in vivo experiment, 40 Ossimi lambs were divided into four groups; the first was control, other groups (T1, T2 and T3) fed diets containing 6% PP, 6% ML or mix levels (3% PP + 3%ML), respectively, for 2 months. Results showed that PP and ML were rich in tannins. In the in vitro test, a maximum reduction in gas, methane and NH3-N was in 6% PP, 6% ML and mixed levels (3% PP + 3% ML). In the in vivo experiment, there were no differences in growth and digestibility of DM and nutrients between treatment groups. Only a lowered DM intake and protein digestibility in lambs fed 6% PP. Gas and methane emission was decreased significantly in lambs fed 6% PP, compared to other groups. TVFAs and NH3-N were decreased for treatment groups. Also, all treatments did not show any pathological changes in liver function or on oxidative stress markers. In conclusion, PP and ML can be used in sheep diets at inclusion levels of 6% and mixture without detrimental effects on general health of Ossimi lambs.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 195-196
Author(s):  
Lily P Hernandez ◽  
James L Dunn ◽  
Joel Wenninghoff ◽  
Crystal L Levesque

Abstract Weaning is a period of pig growth associated with increased incidence of oxidative stress which can impact post-weaning performance. Inclusion of feed additives with antioxidant activity in sow diets has been reported to improve offspring growth beyond the suckling phase. A study assessed yeast cell, mint oil, and ɣ-tocopherol supplementation in sow gestation and lactation diets has on offspring post-weaning performance. A total of 605 piglets (6.14 ± 2.53 kg BW) from 53 sows were randomly allotted to 62 pens, balanced by weight and litter within maternal diet [Control diet (CON), control + yeast cell at 0.15% (YC), control + mint oil at 10 ppm (MO), and control + ɣ-tocopherol at 200 ppm (GT)]. Pens of pigs were given a common diet for 126 d post-wean in a 9-phase feeding regimen. After d29 post-wean, performance of pigs deemed light (&lt; 5.10 kg) and heavy (&gt;7.25 kg) at weaning were followed to d126. Performance was analyzed as randomized complete block with pen as experimental unit and Tukey’s adjustment as means separation test. Pigs from CON sows tended to be lighter pigs from all other treatment groups at weaning and d29 post-wean due to differences in daily gain. Lightweight MO and GT pigs had a heavier weight at d42 (P=0.002) than CON and YC pigs (16.8, 16.7, 19.3, and 19.9 ± 0.77 kg in CON, YC, MO, and GT, respectively). At d70 post-wean, GT pigs tended to be heavier than CON pigs, with YC and MO intermediate. Lightweight pigs from MO sows had greater gain (P=0.04) during the finishing period than all other treatment groups, with GT pigs gaining less. There were no detectable differences in BW during the finishing phase among treatments in heavyweight pigs, however, CON pigs tended (P=0.07) to gain the least. Exposure to mint oil and ɣ-tocopherol during the prenatal and suckling period may provide lasting benefits to light-weight pigs post-wean.


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