Comparison of Methods for the Isolation of Thermotolerant Campylobacter from Poultry

2005 ◽  
Vol 68 (3) ◽  
pp. 616-620 ◽  
Author(s):  
P. TANGVATCHARIN ◽  
S. CHANTHACHUM ◽  
P. KOPAIBOON ◽  
N. INTTASUNGKHA ◽  
M. W. GRIFFITHS
Keyword(s):  
Low Cost ◽  
International Organization ◽  
Control Measures ◽  
Detection Methods ◽  
Effective Control ◽  
Industrialized Countries ◽  
Thermophilic Campylobacter ◽  
Solution Methods ◽  
Human Campylobacteriosis ◽  
Campylobacter Spp

Human campylobacteriosis has become the major cause of foodborne gastroenteritis in industrialized countries. Although there have been numerous studies investigating the prevalence of Campylobacter in animals and raw meat, sensitive and low-cost detection methods are needed to implement effective control measures during primary production and to use as tools in risk assessment studies. Thermophilic Campylobacter spp. in naturally contaminated (n = 64) and inoculated (n = 16) broiler samples were detected using two International Organization for Standardization (ISO)–approved methods. Both enrichment broths (those of Preston and of Park and Sanders) were inoculated with (i) Campylobacter jejuni ATCC35921, (ii) boneless breast from broilers, (iii) boneless breast rinse solution, (iv) boneless breast rinse solution inoculated with C. jejuni ATCC35921 before centrifugation, and (v) boneless breast rinse solution inoculated with C. jejuni ATCC35921 after centrifugation. The results indicated that the Park and Sanders broth was superior to the Preston broth for recovery of Campylobacter spp., and no significant differences (P > 0.05) were found between ISO (meat pieces) and modified ISO (centrifuged chicken rinse solution) methods for the detection of Campylobacter spp.

scholarly journals Antimicrobial Resistance, FlaA Sequencing, and Phylogenetic Analysis of Campylobacter Isolates from Broiler Chicken Flocks in Greece

2021 ◽  
Vol 8 (5) ◽  
pp. 68
Author(s):  
George Natsos ◽  
Niki K. Mouttotou ◽  
Emmanouil Magiorkinis ◽  
Anastasios Ioannidis ◽  
Maria Magana ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Epidemiology ◽  
High Resistance ◽  
Phylogenetic Trees ◽  
Human Infection ◽  
Poultry Meat ◽  
Thermophilic Campylobacter ◽  
Resistance Patterns ◽  
Human Campylobacteriosis ◽  
Campylobacter Spp

Human campylobacteriosis caused by thermophilic Campylobacter species is the most commonly reported foodborne zoonosis. Consumption of contaminated poultry meat is regarded as the main source of human infection. This study was undertaken to determine the antimicrobial susceptibility and the molecular epidemiology of 205 Campylobacter isolates derived from Greek flocks slaughtered in three different slaughterhouses over a 14-month period. A total of 98.5% of the isolates were resistant to at least one antimicrobial agent. In terms of multidrug resistance, 11.7% of isolates were resistant to three or more groups of antimicrobials. Extremely high resistance to fluoroquinolones (89%), very high resistance to tetracycline (69%), and low resistance to macrolides (7%) were detected. FlaA sequencing was performed for the subtyping of 64 C. jejuni and 58 C. coli isolates. No prevalence of a specific flaA type was observed, indicating the genetic diversity of the isolates, while some flaA types were found to share similar antimicrobial resistance patterns. Phylogenetic trees were constructed using the neighbor-joining method. Seven clusters of the C. jejuni phylogenetic tree and three clusters of the C. coli tree were considered significant with bootstrap values >75%. Some isolates clustered together were originated from the same or adjacent farms, indicating transmission via personnel or shared equipment. These results are important and help further the understanding of the molecular epidemiology and antimicrobial resistance of Campylobacter spp. derived from poultry in Greece.

scholarly journals Seasonal variability of thermophilic Campylobacter spp. in raw milk sold by automatic vending machines in Lombardy

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Barbara Bertasi ◽  
Marina Nadia Losio ◽  
Paolo Daminelli ◽  
Guido Finazzi ◽  
Andrea Serraino ◽  
...  
Keyword(s):  
Raw Milk ◽  
Human Infection ◽  
Seasonal Trend ◽  
Fecal Shedding ◽  
Vending Machines ◽  
Thermophilic Campylobacter ◽  
Wide Range ◽  
Significant Difference ◽  
Human Campylobacteriosis ◽  
Campylobacter Spp

In temperate climates, a seasonal trend was observed in the incidence of human campylobacteriosis cases, with peaks reported in spring and autumn in some countries, or in summer in others; a similar trend was observed in <em>Campylobacter</em> spp. dairy cattle fecal shedding, suggesting that cattle may play a role in the seasonal peak of human infection. The objectives of this study were to assess if a seasonal trend in thermophilic <em>Campylobacter</em> spp. contamination of raw milk exists and to evaluate a possible relation between this and the increase of human campylobacteriosis incidence in summer months. The results showed a mean prevalence of 1.6% of milk samples positive for thermophilic <em>Campylobacter</em> spp. with a wide range in different months during the three years considered of 0.0-3.1%. The statistical analysis showed a significant difference (P&lt;0.01) of the prevalence of positive samples for thermophilic <em>Campylobacter</em> spp. between warmer and cooler months (2.3% <em>vs</em> 0.6%). The evidence of a seasonal trend in thermophilic <em>Campylobacter</em> spp. contamination of raw milk sold for direct consumption, with an increase of the prevalence in warmer months, may represent one of the possible links between seasonal trend in cattle fecal shedding and seasonal trend in human campylobacteriosis.

Prevalence of thermophilic Campylobacter spp. in non-human primates of the Macaca mulatta species from a breeding center for use as biomodels

2018 ◽  
Vol 50 (1) ◽  
Author(s):  
Paula Borba Cruz ◽  
Fernanda Nunes Santos ◽  
Wagner Thadeu Cardoso Esteves ◽  
Jaime Antonio Abrantes ◽  
Fabio Alves da Silva ◽  
...  
Keyword(s):  
Macaca Mulatta ◽  
Thermophilic Campylobacter ◽  
Breeding Center ◽  
Campylobacter Spp

Characterizing the effectiveness of COVID-19 mitigation measures: A data-centric approach (Preprint)

2020 ◽  
Author(s):  
Lukman Olagoke ◽  
Ahmet E. Topcu
Keyword(s):  
Control Measure ◽  
Control Measures ◽  
World Health ◽  
Mitigation Measures ◽  
Effective Control ◽  
The World ◽  
Decision Making Processes ◽  
And Performance ◽  
Health Organization ◽  
The Impact

BACKGROUND COVID-19 represents a serious threat to both national health and economic systems. To curb this pandemic, the World Health Organization (WHO) issued a series of COVID-19 public safety guidelines. Different countries around the world initiated different measures in line with the WHO guidelines to mitigate and investigate the spread of COVID-19 in their territories. OBJECTIVE The aim of this paper is to quantitatively evaluate the effectiveness of these control measures using a data-centric approach. METHODS We begin with a simple text analysis of coronavirus-related articles and show that reports on similar outbreaks in the past strongly proposed similar control measures. This reaffirms the fact that these control measures are in order. Subsequently, we propose a simple performance statistic that quantifies general performance and performance under the different measures that were initiated. A density based clustering of based on performance statistic was carried out to group countries based on performance. RESULTS The performance statistic helps evaluate quantitatively the impact of COVID-19 control measures. Countries tend show variability in performance under different control measures. The performance statistic has negative correlation with cases of death which is a useful characteristics for COVID-19 control measure performance analysis. A web-based time-line visualization that enables comparison of performances and cases across continents and subregions is presented. CONCLUSIONS The performance metric is relevant for the analysis of the impact of COVID-19 control measures. This can help caregivers and policymakers identify effective control measures and reduce cases of death due to COVID-19. The interactive web visualizer provides easily digested and quick feedback to augment decision-making processes in the COVID-19 response measures evaluation. CLINICALTRIAL Not Applicable

scholarly journals Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 3985
Author(s):  
Nan Wan ◽  
Yu Jiang ◽  
Jiamei Huang ◽  
Rania Oueslati ◽  
Shigetoshi Eda ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Clinical Diagnostics ◽  
Detection Methods ◽  
Capacitive Sensing ◽  
Application Potential ◽  
Mirna Detection ◽  
Mirna 25 ◽  
Low Sensitivity

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.

A UN Specialized Agency for the Environment

2021 ◽  
pp. 1-10
Author(s):  
Said Mahmoudi
Keyword(s):  
International Law ◽  
Financial Stability ◽  
Environmental Governance ◽  
Institutional Arrangement ◽  
International Organization ◽  
Main Question ◽  
Industrialized Countries ◽  
Institutional Reforms ◽  
Global Environmental ◽  
Stockholm Conference

The issue, international organization for the protection of the environment perhaps more than those in any other area of international law, is characterized by the contestation of the policies and aspirations of developing and industrialized countries. The discussions which preceded the 1972 Stockholm Conference concerned partly the type of international institutional arrangement required for addressing the environmental problems. As regards the institutional reforms with respect to international environmental governance (IEG), the main question is whether to focus on the existing global institution, i.e. UNEP, or to create a new functional international organization. After almost five decades of existence, turning UNEP into a ‘specialized agency’ within the UN system is a reasonable move. It would meet the long-felt need to elevate its status and equip it with the necessary competence and financial stability for the demanding task it should have as an efficient global environmental organization.

scholarly journals Effectiveness of Epidemic Preventive Policies and Hospital Strategies in Combating COVID-19 Outbreak in Taiwan

2021 ◽  
Vol 18 (7) ◽  
pp. 3456
Author(s):  
Ting Wan Tan ◽  
Han Ling Tan ◽  
Man Na Chang ◽  
Wen Shu Lin ◽  
Chih Ming Chang
Keyword(s):  
Mortality Rate ◽  
Incidence Rate ◽  
Risk Ratio ◽  
Early Stage ◽  
Control Measures ◽  
Polymerase Chain Reaction Test ◽  
Effective Control ◽  
Nasopharyngeal Swab ◽  
Preventive Strategies ◽  
Epidemic Control

(1) Background: The implementation of effective control measures in a timely fashion is crucial to control the epidemic outbreak of COVID-19. In this study, we aimed to analyze the control measures implemented during the COVID-19 outbreak, as well as evaluating the responses and outcomes at different phases for epidemic control in Taiwan. (2) Methods: This case study reviewed responses to COVID-19 and the effectiveness of a range of control measures implemented for epidemic control in Taiwan and assessed all laboratory-confirmed cases between 11 January until 20 December 2020, inclusive of these dates. The confirmation of COVID-19 infection was defined as the positive result of a reverse-transcriptase–polymerase-chain-reaction test taken from a nasopharyngeal swab. Test results were reported by the Taiwan Centers for Disease Control. The incidence rate, mortality rate, and testing rate were compiled, and the risk ratio was provided to gain insights into the effectiveness of prevention measures. (3) Results and Discussion: This study presents retrospective data on the COVID-19 incidence rate in Taiwan, combined with the vital preventive control measures, in a timeline of the early stage of the epidemic that occurred in Taiwan. The implementation of multiple strategy control measures and the assistance of technologies to control the COVID-19 epidemic in Taiwan led to a relatively slower trend in the outbreak compared to the neighboring countries. In Taiwan, 766 confirmed patients were included, comprised of 88.1% imported cases and 7.2% local transmission cases, within the studied period. The incidence rate of COVID-19 in Taiwan during the studied period was 32 per million people, with a mortality rate of 0.3 per million people. Our analysis showed a significantly raised incidence risk ratio in the countries of interest in comparison to Taiwan during the study period; in the range of 1.9 to 947.5. The outbreak was brought under control through epidemic policies and hospital strategies implemented by the Taiwan Government. (4) Conclusion: Taiwan’s preventive strategies resulted in a drastically lower risk for Taiwan nationals of contracting COVID-19 when new pharmaceutical drug or vaccines were not yet available. The preventive strategies employed by Taiwan could serve as a guide and reference for future epidemic control strategies.

scholarly journals Molecular Identification of Mushroom Species in Italy: An Ongoing Project Aimed at Reinforcing the Control Measures of an Increasingly Appreciated Sustainable Food

2020 ◽  
Vol 13 (1) ◽  
pp. 238
Author(s):  
Alice Giusti ◽  
Enrica Ricci ◽  
Laura Gasperetti ◽  
Marta Galgani ◽  
Luca Polidori ◽  
...  
Keyword(s):  
Selection Process ◽  
Winning Strategy ◽  
Control Measures ◽  
Effective Control ◽  
Mushroom Poisoning ◽  
Clinical Samples ◽  
Similar Species ◽  
Mushroom Species ◽  
Ongoing Research ◽  
Genotypic Analysis

Proper investment in mushroom production (farming and wild mushroom picking activities) may represent a winning strategy for many countries, including Italy, to better face the problems of food security and environmental impact, and to break away from imports, enhancing the local products. However, the risk related to the consumption of poisoning species requires governments to implement or reinforce effective control measures to protect consumers. Mushroom identification by phenotype observation is hardly applicable if morphologically-similar species, non-whole specimens, or clinical samples are involved. Genotypic analysis is a valid alternative. An ongoing research project involving the Experimental Zooprophylactic Institute of Lazio and Tuscany, the regional Mycological Inspectorate, the Tuscany Mycological Groups Association, and the Department of Veterinary Sciences of the University of Pisa aims to reinforce the collaboration among institutions for the management of mushroom poisoning. The core’s project aims to develop an internal genetic database to support the identification of wild and cultivated mushroom species in the Italian territory. The database will include Internal Transcribed Spacer (ITS) sequences retrieved from official databases (the NCBI GenBank and the BOLD system) which are considered to be reliable, after a proper selection process, and sequences from specimens collected directly and identified by expert mycologists. Once it is validated, the database will be available and further implementable by the official network of national laboratories.

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