scholarly journals Carotenoid, Carotene and Anthocyanin Levels of Naturally Grown old Garden Roses (Rosa ssp.) in Van

2020 ◽  
Vol 4 (1) ◽  
pp. 25-30
Author(s):  
Füsun GÜLSER ◽  
Şevket ALP ◽  
Ferit SÖNMEZ
Keyword(s):  
Antimicrobial Activity ◽  
Bioactive Compounds ◽  
Plant Material ◽  
Aqueous Acetone ◽  
Rosa Damascena ◽  
Rosa Multiflora ◽  
Physiological Importance ◽  
Bioactive Content ◽  
Eleven Species

Secondary metabolites especially phenolic compounds, flavonoids, tannins, and terpenoids have potential antioxidants and antimicrobial activity. Carotenoids are important antioxidants and bioactive compounds supplying to the health benefits of different foods and rosehips known for high bioactive content. Anthocyanins have physiological importance because of their abilities to protect leaves from photooxidative injuries without significantly compromising photosynthesis. Numerous studies shown that roses are rich sources of bioactive compounds. The genus Rosa contains various species that are widely grown in Europe, Asia, The Middle East and North America. In this study it was aimed that determination of carotenoid, carotene and anthocyanin amounts of naturally growing old garden roses species in Van ecological conditions. In this study eleven species of rosehips (Rosa damascena, Rosa damascena var. semperflorens, Rosa laxa ssp. harputensis, Rosa alba, Rosa multiflora, Rosa pisiformis, Rosa foetida, Rosa foetida var. bicolor, Rosa hemisphaerica, Rosa hemisphaerica var. plena hort, Rosa heckellana subsp. vanheurckiona) naturally grown in Van conditions were used as plant material. The fresh leaves (100-200 mg), immediately after harvesting, were homogenized by 80% aqueous acetone and filtered by white tape filter paper. In the extracts, carotenoid, carotene and anthocyanin levels were determined by using UV spectrophotometer in 470 nm, 537 nm, 647 nm, 663 nm wavelengths respectively. As a result, the highest and lowest carotenoid levels were found as 3.18 µg/ml and 0.59 µg/ml in R. heckellana subsp. vanheurckiona and R. damascena var. semperflorens. The highest anthocyanin level was determined in R. damascena as 0.06 µg/ml.

scholarly journals Antimicrobial Study of Chloroform Fraction from the Leaves of Entada spiralis Ridl.

2021 ◽  
Vol 1 (1) ◽  
pp. 45-53
Author(s):  
Fitri Rachmadita ◽  
Erryana Martati ◽  
Sharifah Nurul Akilah Syed Mohamad ◽  
Siti Zaiton Mat So'ad
Keyword(s):  
Antimicrobial Activity ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Inhibition Zone ◽  
Food Preservation ◽  
Total Phenolic ◽  
Chloroform Fraction ◽  
Disk Diffusion Test ◽  
E Coli

Introduction: Entada Spiralis Ridl., or locally identified as Sintok, contains flavonoid, saponin, tannin, and glycoside, compounds that have antifungal and antibacterial activities. This research aims to identify bioactive compounds and determine the antimicrobial activity from crude and fraction of E. spiralis extract. Methods: The crude extract was prepared by macerating the leaves with chloroform, and then proceeded to fraction it by vacuum liquid chromatography with Dichloromethane (DCM)/Hexane (Hex) (1/9) and Dichloromethane (DCM)/Methanol (MeOH) (9/1) solvent system. Disk Diffusion Test and Microdilution Assay evaluated the extracts' antimicrobial activity against S. aureus, E. coli and C. albicans. The determination of bioactive compounds was done by Thin Layer Chromatography (TLC). Determination of Total Phenolic (TPC) and Flavonoid Content (TFC) were performed by Folin-Ciocalteu and AlCl3 Colourimetric Assay Results: The greatest inhibition zone against C. albicans was obtained from fraction Chloroform (CHCl3) extract with an inhibition zone of 10.33 mm. DCM/MeOH (9/1) effectively killed S. aureus and E.coli with an inhibition zone of 11.67 and 12 mm, respectively. The minimum inhibitory concentration (MIC) of CHCl3 crude extract were 1.563 mg/mL for both E. coli and S. aureus, and 0.781 mg/mL for C. albicans. The TLC revealed the presence of tannins, saponin, glycosides, phenol, flavonoid, triterpenoid, and aromatic compound in CHCl3 crude extract. TPC of DCM/MeOH (9/1), CHCl3, and DCM/Hex (1/9) were 50.56 ± 0.188, 51.913± 0.089, 24.16 ± 0.175 mg GAE/g extract. Conclusion: In conclusion, E. spiralis leaves could be a source of active antifungal and antimicrobial agents used for food preservation by using a semipolar solvent for extraction.

scholarly journals The identification and quantification of bioactive compounds from the aqueous extract of comfrey root by UHPLC-DAD-HESI-MS method and its microbial activity

2015 ◽  
Vol 69 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Vesna Savic ◽  
Sasa Savic ◽  
Vesna Nikolic ◽  
Ljubisa Nikolic ◽  
Stevo Najman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Microbial Activity ◽  
Bioactive Compounds ◽  
Aqueous Extract ◽  
Ellagic Acid ◽  
Rosmarinic Acid ◽  
Qualitative And Quantitative ◽  
Quantitative Analyses

In this study the qualitative, quantitative and microbial determination of the aqueous extract of comfrey root was done. The qualitative and quantitative analyses were done by the UHPLC-DAD-HESI-MS method. As major bioactive compounds, allantoin, rosmarinic acid and ellagic acid were identified and their quantification was also done. The obtained results showed a high content of allantoin, ellagic acid and rosmarinic acid (8.91, 7.4 and 12.8%, respectively) which indicated that the comfrey root can be used as a source for the isolation of these three compounds. The results obtained by the determination of the antimicrobial activity showed that Escherichia coli ATCC8739 and Salmonela typhimirium ATCC6538 were most sensitive to the aqueous extract of comfrey root. The results showed that allantoin did not express the antimicrobial activity on all the investigated bacteria species, and based on this it can be concluded that allantoin is not responsible for the antimicrobial activity of the aqueous extract of comfrey root.

Determination of Oligoelements Content of Plant Material and Assessment of Bioactive Compounds from Calendula officinalis Lyophilized Extract

2017 ◽  
Vol 68 (8) ◽  
pp. 1786-1789
Author(s):  
Tunde Jurca ◽  
Eleonora Marian ◽  
Bogdan Tita ◽  
Simona Vicas ◽  
Annamaria Pallag ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Bioactive Compounds ◽  
Working Conditions ◽  
Plant Material ◽  
Polyphenolic Compounds ◽  
Icp Oes ◽  
Calendula Officinalis ◽  
Hydroalcoholic Extract ◽  
Complete Extraction

The marigold extracts are used in traditional medicine for its anti-inflammatory, decongestive, antiseptic, antifungal, antimicrobial and immunostimulating properties. Existing studies have partially proved these properties, but are incomplete with regard to the possibility of standardizing the extract using the best working conditions so as it to be included in dietary supplements. The purpose of this study is to investigate the chemical composition of the marigold extracts, their contents in flavonoids and polyphenolic compounds, as well as to determine the contents in oligoelements of the plant material through the ICP-OES method. Based on the results of this study we conclude that a complete extraction may be accomplished after lyophilizing the solution of hydroalcoholic extract, followed by extraction in acid medium so as to permit a better assessment of the bioactive compounds.

Extraction and Phytochemicals Determination of Traditional Medicinal Plants for Anti-microbial Susceptibility Test

2020 ◽  
Vol 2020 ◽  
Author(s):  
Firew Admasu
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Traditional Medicine ◽  
Crude Extract ◽  
Susceptibility Test ◽  
Plant Sample ◽  
Traditional Medicinal Plants ◽  
Traditional Health Care ◽  
Susceptibility Tests

Abstract: The study were conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants contributors to pharmaceutical, agricultural and food industries in the world. The use of medicinal plants in the industrialized societies has been traced to extraction and development of several drugs used in order to heel some diseases having inhibiting effect against pathogenic microorganism. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for anti microbial susceptibility test. Methodology: The extraction and identification of some phytochemicals crude compound which used for antimicrobial susceptibility test from plant sample such as Ocimum lamiifolium (OL), Croton maerosth (Cm) and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortal and pistil and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compound. Result: The main result of Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to managing numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role by reducing life-threatening ailments of people and other animals.

Assessment of Antimicrobial Activity and total Polyphenol Content of Rosa multiflora and Rosa wichuraiana

2019 ◽  
Vol 10 (04) ◽  
pp. 646-650
Author(s):  
Hyeusoo Kim ◽  
Kyeong Won Yun
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Fraction ◽  
Rosa Multiflora ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Water Fraction ◽  
Total Polyphenol Content ◽  
Rosa Species

The fruit of Rosa multiflora has been used as traditional herbal medicine in Asian countries. The present investigation was undertaken to study the antimicrobial activity and total polyphenol content of hexane, ether, ethyl acetate, water fraction of methanol extract of fruit and flower from Rosa multiflora and Rosa wichuraiana. Antimicrobial activity of the mentioned fractions against 3 gram-positive and 4 gram-negative bacteria using disk diffussion method. The measurement of minimal inhibition concentration (MIC) showed that the ethyl acetate fraction of the two Rosa species is the most effective against the tested bacteria. The total polyphenol content of ethyl acetate fraction of the two Rosa species is higher than the other fractions. The results indicate the antimicrobial activity was related with the total polyphenol content and the fruit and flower of the two Rosa species can be considered as a natural source of antimicrobial agents.

Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis

2019 ◽  
Vol 70 (6) ◽  
pp. 2015-2020
Author(s):  
Silvia Robu ◽  
Aurelia Romila ◽  
Olimpia Dumitriu Buzia ◽  
Adrian Florin Spac ◽  
Camelia Diaconu ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Plant Material ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Salvia Officinalis ◽  
Salvia Officinalis L ◽  
Gas Chromatographic Method

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.

The Comparative Study of Equisetum pratense, E. sylvaticum, E. telmateia: Accumulation of Silicon, Antioxidant and Antimicrobial Screening

2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Previous Treatment ◽  
Total Phenolic ◽  
Quantitative Chemical Analysis ◽  
Qualitative And Quantitative ◽  
Antioxidant And Antimicrobial Activity ◽  
The Comparative Study

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).

Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

2020 ◽  
Vol 16 ◽  
Author(s):  
Natasa P. Kalogiouri ◽  
Natalia Manousi ◽  
Erwin Rosenberg ◽  
George A. Zachariadis ◽  
Victoria F. Samanidou
Keyword(s):  
Fatty Acids ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
High Performance ◽  
Unsaturated Fatty Acids ◽  
Nutritional Deficiencies ◽  
Analytical Technique ◽  
High Volatility ◽  
Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

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