scholarly journals Study of Hematological Characteristics and Mineral Metabolism of Moscow Black Chickens in the Process of Acclimatization in the Astrakhan Region

2022 ◽  
Vol 16 ◽  
pp. 9-13
Author(s):  
Bratashova Tatyana Sergeevna ◽  
Zakharkina Natalya Ivanovna ◽  
Shcherbakova Elena Nikolaevna ◽  
Safonov Vladimir Aleksandrovich
Keyword(s):  
Trace Elements ◽  
Blood Cells ◽  
Mineral Metabolism ◽  
White Blood Cells ◽  
Climatic Conditions ◽  
Feed Additives ◽  
Moscow Region ◽  
Lower Volga Region ◽  
Low Levels ◽  
Hematological And Biochemical Parameters

The study of indicators characterizing the processes associated with the acclimatization of birds in new biogeochemical conditions is of interest in connection with the prospects of the poultry industry in the Astrakhan Region, where the lack of important trace elements in soils and plant feeds: Se, I, Co, is observed. In the presented study, the main hematological and biochemical parameters of blood and mineral metabolism were determined in 2 groups of chickens of the Moscow black breed: imported to the Astrakhan Region from Moscow and kept in the Moscow Region. The analyses revealed strong differences (P<0.01) in indicators of two groups. The acclimatized birds were characterized by low levels of red blood cells and hemoglobin in the blood, as well as a higher content of white blood cells than in the second group. The chickens had impaired lipid and protein metabolism. A 71.3% increase in the level of total sugar most likely indicated a stress state of birds. Compared with the chickens in the Moscow Region, the level of calcium in their blood was 32.4% lower, phosphorus – 12.9%. To a large extent, a decrease in the levels of iodine and selenium – by 5.3 and 5.2 times, respectively, was observed. Thus, in the absence of external changes, acclimatized chickens are subject to a latent form of combined I- and Se-hypomicroelementosis. At the same time, a drop in egg productivity by 19% is the recorded fact. The necessity to adapt to the new climatic conditions of the Lower Volga region for birds, combined with a lack of important trace elements, can cause increased oxidative stress in acclimatized birds. Based on the results of the study, the use of feed additives that make up for the deficiency of necessary trace elements is recommended.

scholarly journals Effects of static magnetic field exposure on hematological and biochemical parameters in rats

2006 ◽  
Vol 49 (6) ◽  
pp. 889-895 ◽  
Author(s):  
Salem Amara ◽  
Hafedh Abdelmelek ◽  
Mohamed Ben Salem ◽  
Rached Abidi ◽  
Mohsen Sakly
Keyword(s):  
Magnetic Field ◽  
Static Magnetic Field ◽  
Growth Rates ◽  
Glucose Concentration ◽  
Blood Cells ◽  
White Blood Cells ◽  
Male Rats ◽  
Field Exposure ◽  
Protein Levels ◽  
Hematological And Biochemical Parameters

The present work was undertaken in order to investigate the effects of static magnetic field (SMF) on growth rates, hematopoiesis, plasmatic proteins levels, glucose concentration, lactate dehydrogenase (LDH) and transaminases activities in male rats. Sub-acute exposure of rats during 5 consecutive days to SMF (1h/day at 128mT) induced an increase of plasma LDH activity (+38%, p<0.05), and glucose concentration (+31%, p<0.05), whereas haematological parameters, protein levels, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities remained unchanged. SMF exposure of rats during 30 consecutive days (1hour/day at 128mT) decreased significantly growth rates by the second week and increased significantly the plasmatic total protein levels (+62%, p<0.05), hemoglobin (+10%, p<0.05), red blood cells (+7%, p<0.05), white blood cells (+17%, p<0.05), and platelet number (+10%, p<0.05). Sub-chronic exposure to SMF increased also LDH (+43%, p<0.05), AST (+ 41%, p<0.05) and ALT activities (+95%, p<0.05). In contrast, the glucose concentration was unaffected. These changes suggested that exposure to SMF had a possible effect on the proliferation of blood cells and enzymes release within blood indicating tissue alterations.

The Characterization of DYRK3 mRNA and the Utility of DYRK3 Inhibitors in a Carboplatin/Radiation Mouse Model of Anemia.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2202-2202
Author(s):  
Connie L. Erickson-Miller ◽  
Ying Homan ◽  
Matthew Chomo ◽  
Elizabeth I. Valoret ◽  
Louis Elefante ◽  
...  
Keyword(s):  
Single Dose ◽  
Mouse Model ◽  
Blood Cells ◽  
Radiation Treatment ◽  
White Blood Cells ◽  
Negative Regulator ◽  
Mrna Levels ◽  
Erythroid Progenitors ◽  
Low Levels

Abstract DYRK3, a member of the dual-specificity tyrosine phosphorylation-regulated kinase family, is expressed at low levels in erythroid progenitors and is implicated as a negative regulator of erythropoiesis. An appropriate animal model of anemia was sought to investigate the potential utility of DYRK3 inhibitors as a therapy for the treatment of anemia. Treatment of mice with sub-lethal irradiation followed by a single dose of the chemotherapeutic, nucleoside analog carboplatin, is well known to induce severe anemia. In the first instance, an analysis of DYRK3 mRNA levels was performed to ascertain whether the anemia so induced results in increased DYRK3 transcription. In this carboplatin/radiation model, mice were exposed to sub-lethal gamma irradiation (500 rads) followed by a single dose of carboplatin (60 mg/kg). This treatment induced an approximately 50% decrease in hemoglobin with concomitant drops in other erythroid parameters (hematocrit and RBC) by days 15–17. The following parameters were examined in five animals per day: peripheral blood counts, marrow cell count, plasma Epo levels, marrow Ter119+/CD71+ expression and marrow DYRK3 mRNA. As expected, the hemoglobin, hematocrit and red blood cells decreased gradually to nadir on day 15. White blood cells decreased to very low levels within 2 days of carboplatin/radiation treatment and remained suppressed for 11 days. Platelets decreased to nadir at day 7, where they remain until day 10. Plasma Epo levels were low and abruptly increased at day 3–4. The absolute number of Ter119+/CD71+ cells immediately dropped from normal levels at day 1 and then increased at day 6 and then fluctuated between a 30- to 60- fold enhancement from day 8 through 21 when the study was complete. DYRK3 mRNA, as measured by quantitative PCR (Taqman), increased approximately 10-fold at day 7 and remained in that range until day 21. The number of erythroid progenitors measured by flow cytometry,Ter119+/CD71+ cells, and the level of DYRK3 mRNA remained elevated until the end of the experiment at day 21, at which point the hemoglobin had recovered to near normal levels. GSK626616, a potent, low molecular weight inhibitor of DYRK3 kinase activity (IC50 = 0.7 nM), was dosed once daily, i.p., for 17 days in this model. At day 15, the GSK626616-treated mice (0.03 mg/kg) demonstrated a statistically significant increase in hemoglobin, hematocrit, red blood cell and platelet counts compared to anemic animals treated with vehicle alone. In contrast to its effects in anemic mice, this compound demonstrated no increases in any blood parameters in normal mice over a similar timeframe and dosage regimen. This expected behavior is hypothesized to be due to the low level of DYRK3 mRNA in normal, non-anemic mice. The characterization of this carboplatin/radiation mouse model demonstrates that as the hemoglobin decreased, plasma Epo increased at day 3–4, followed by the increase in Ter119+/CD71+ cells at day 6. Following this surge in erythropoiesis, an increase in DYRK3 mRNA expression naturally follows. The subsequent improved erythropoiesis in animals treated with a DYRK3 inhibitor in this model, suggests that DYRK3 kinase mRNA levels could have utility as a biomarker in the identification of an anemic patient population that then may be a candidate for treatment with a DYRK3 inhibitor.

Multicenter evaluation of two flow cytometric methods for counting low levels of white blood cells

Transfusion ◽  
2004 ◽  
Vol 44 (9) ◽  
pp. 1319-1324 ◽  
Author(s):  
Margriet J. Dijkstra-Tiekstra ◽  
Pieter F. Van Der Meer ◽  
Ruby N.I. Pietersz ◽  
Janny De Wildt-Eggen
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Flow Cytometric ◽  
Low Levels

scholarly journals STUDIES ON THE PATHOGENESIS OF ACUTE INFLAMMATION

1960 ◽  
Vol 111 (1) ◽  
pp. 45-64 ◽  
Author(s):  
Fred Allison ◽  
Margie G. Lancaster
Keyword(s):  
Blood Cells ◽  
Blood Clotting ◽  
Acute Inflammation ◽  
White Blood Cells ◽  
Blood Stream ◽  
Blood Levels ◽  
Primary Role ◽  
Low Levels

The relation of intravascular fibrin to the leucocytic sticking reaction in ear chambers of rabbits injured by heat was investigated in two ways. First, attempts were made to destroy the thin layer of fibrin believed to coat the surfaces of cells involved in the sticking reaction. Second, white cell sticking was studied after fibrinogen had been removed from the blood stream. The results of these experiments were as follows:— 1. Activation of fibrinolysin in vivo by streptokinase did not impair sticking of white blood cells. 2. Administration of streptokinase parenterally did not lower fibrinogen blood levels appreciably even when the amount used was large. 3. Thromboplastin infusions alone reduced circulating fibrinogen to low levels but leucocytic sticking was not prevented. Furthermore, frequent death of animals due to pulmonary embolism made such experiments prohibitive. 4. Addition of streptokinase to thromboplastin infusions protected against embolic deaths but did not influence sticking even though the fibrinogen levels achieved were quite low. 5. Finally, when thrombin was added to infusions of thromboplastin and streptokinase, no circulating fibrinogen could be detected. Under such circumstances leucocytic sticking following heat injury occurred without reduction. These findings were interpreted as evidence against a primary role of the blood clotting mechanism in causing the sticking of white blood cells to injured endothelium. Alternative explanations were discussed.

scholarly journals Morphofunctional and Hematological Characteristics of Health in Students from the Northern and Southern Regions of Kazakhstan

2021 ◽  
Vol 9 (A) ◽  
pp. 753-759
Author(s):  
Saule Bazarbaeva ◽  
Aigul Dinmukhamedova ◽  
Gulnara Tleubergenova ◽  
Zhanar Rakhimzhanova ◽  
Kamila Sembekova ◽  
...  
Keyword(s):  
Health Status ◽  
Blood Cells ◽  
White Blood Cells ◽  
Health Condition ◽  
Northern Region ◽  
Climatic Conditions ◽  
Southern Region ◽  
First Year ◽  
First Year Students ◽  
Significant Difference

The study aimed at investigating morphofunctional and hematological features of the first-year students’ health condition. The health status for a total of 400 first-year students, who arrived for studying from the northern region (North Kazakhstan) and the southern region (Turkestan, Kyzylorda), was estimated during the study. The morphological (BMI, vital lung capacity, etc.) and hematological (the total number of red blood cells, white blood cells, the concentration of hemoglobin in the blood, etc.) indicators of the health status were investigated. By comparing the condition of physical health in students from both regions, a significant difference in main morphofunctional and hematological indices has been established. The functional indicators of the cardiovascular and respiratory systems were higher in students from the southern region, which reflects a good adaptive reaction to studying at a university, as well as new climatic and geographical conditions. Students from the southern region were reported to have lower red blood cell amounts and diagnosed with mild anemia compared to those from the northern region. Therefore, constant monitoring of the health condition among youth is required to develop effective tactics for the students’ smooth adjusting to living in new climatic conditions.

scholarly journals The effect of plant hormone indoleacetic acid (IAA) on hematological and biochemical parameters in mice

1970 ◽  
pp. 5-8 ◽  
Author(s):  
MH Morshed ◽  
M Shamm Hossain ◽  
M Anwar Habib ◽  
MM Moinuddin Ahmed ◽  
M Ibrahim ◽  
...  
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Plant Hormone ◽  
Indoleacetic Acid ◽  
White Blood Cells ◽  
Control Group ◽  
Differential Count ◽  
General Observation ◽  
Hematological And Biochemical Parameters ◽  
Serum Glutamate

The subacute toxicity of plant hormone indoleacetic acid (IAA) was studied on Swiss albino mice. The studies include the gross general observation such as changes in body weight, hematological profiles [total count of red blood cells (RBC) and white blood cells (WBC), differential count of WBC, platelet count, hemoglobin (Hb)%], biochemical parameters of blood [serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phospatase(SALP), creatinine] and histopathology of the liver, kidney, lung, spleen, heart and brain of both control and experimental day-1 for consecutive 14 days, showed no significant change of hematological and biochemical parameters. No abnormalities were also found in the histopathology of the liver, kidney, lung, spleen, heart and brain in the experimental group of animals following same dose when compared with control group. This preliminary toxicological study suggests that the plant hormone indoleacetic acid (IAA) may be used safely for agricultural purposes and as an external preservative DOI: 10.3329/bjpp.v22i1.3561 Bangladesh J Physiol Pharmacol 2006; 22(1/2) : 5-8  

scholarly journals The effectiveness of new feed additives on the basis of lactulose in growing rabbits

2020 ◽  
Vol 12 ◽  
pp. 52-60
Author(s):  
O.A. Knyazhеchenko ◽  
◽  
I.A. Semenova ◽  
A.A. Mosolov ◽  
M.V. Frolova ◽  
...  
Keyword(s):  
Blood Cells ◽  
Positive Impact ◽  
Physiological State ◽  
White Blood Cells ◽  
Live Weight ◽  
Feed Additives ◽  
The Body ◽  
Natural Resistance ◽  
Control Group ◽  
Absolute Increase

Aim. To study the feasibility of using feed additives containing powdered feed preparations with different lactulose content in the amount of 5 g/kg of extruded compound feed together with the main diet in terms of the effect on the quality indicators of raw materials and the natural resistance of the body of experimental rabbits. Materials and Methods. The effectiveness was evaluated after the control slaughter at the age of 150 days in the number of 5 heads from each group. The change in live weight was determined when the rabbits reached the age of 75, 105, 135 and 150 days by weighing on an electronic scale. The physiological state and resistance of rabbits were evaluated by hematological parameters. The obtained materials were processed by the methods of variation statistics. Results. The analysis of dynamics of changes of alive weight of animals showed that feed supplementation with lactulose had a positive impact on the growth of animals the live weight of rabbits in groups I and II were greater at 3.8 and 6.2% absolute increase in live weight during the period of experience increased by 5.3 and 7.9%, respectively, compared to control. At the same time, the effect of supplements on blood parameters should also be noted. In our studies, the rabbits of the experimental groups had an advantage over the rabbits of the control group in terms of the level of red blood cells by 0.94·1012 g/l (18.7%) and 1.12·1012 g/l; white blood cells - by 0.50·109 (7.2%) g/l and 0.59·109 g/l (8.5%), respectively. The level of hemoglobin in the blood of rabbits increased by the end of the experiment in the control group by 3.7%, in I – by 12.5%, in II – by 15.9%. Conclusion. Based on the presented results, it can be concluded that the data on quantitative changes in blood were due to the influence of feed additives, which contributed to an increase in the natural resistance of rabbits, and the best indicators were achieved by rabbits of the II experimental group.

scholarly journals EFFICACY OF IVERMACTIN AGANIST SOME NATURALLY ACQUIRED GASTRO-INTESTINAL PARASITIC INFECTION IN MULES

2022 ◽  
Vol 21 (1) ◽  
pp. 100-111
Author(s):  
Amir n. Saleem
Keyword(s):  
Blood Cells ◽  
Normal Values ◽  
Intestinal Parasites ◽  
White Blood Cells ◽  
Parasitic Infection ◽  
Intestinal Parasitic Infection ◽  
Erythrocyte Sedimentation ◽  
Faecal Samples ◽  
Low Levels ◽  
Strongyloides Westeri

The activity of a single subcutaneous injection of ivermectin at dose of 200 mcg/kg of body weight was evaluated against naturally acquired gastro-intestinal parasites in mules. Faecal samples were examined at the time of treatment and weekly thereafter up to 4 weeks. Results indicated that invrmectin was highly effective (100%) against Oxyuries equi, Strongyloides westeri, Tristostronglus axei, Trichhonema spp., and Strongylus spp. as judged one week after ivermectin administration.  Complete elimination of Parascaris equorum eggs occurred two weeks after treatment. In contrast, ivermectin was not effective against the tape worms, Anoplocephala spp.. Infected animals had low levels of haemoglobin and red blood cells counts and an elevated packed cell volume, white blood cells and erythrocyte sedimentation rate.  These values returned to normal values 21 days post treatment. No adverse reaction was recorded in the treated animals.

STUDY OF RESISTANCE OF THE PIG BODY WHEN USING A COMPLEX MICROFEED PRODUCT IN NUTRITION

2021 ◽  
Vol 1 (1) ◽  
pp. 86-90
Author(s):  
L.A. Nikanova ◽  
Keyword(s):  
Green Algae ◽  
Blood Cells ◽  
White Blood Cells ◽  
Live Weight ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Blue Green Algae ◽  
Organic Iodine ◽  
Experimental Group

The object of research is the components of feed additives, namely dihydroquercetin, blue-green algae spirulina and organic iodine, in various combinations used in feeding piglets from the first days of feeding and before transferring them to fattening, at the age of 90 days. Three groups of piglets, one control and two experimental ones, were formed according to the principle of analogues. Piglets of the first experimental group received to the general diet, a feed additive MKBA-1, consisting of Spirulina – 1 mg, dihydroquercetin – 3 mg, organic iodine – 1,5 mg per 1,5 kg of live weight per day; piglets of the second experimental group to the main diet received a feed supplement ICBA-1, consisting of Spirulina – 2 mg and dihydroquercetin – 2 mg per 3 kg of live weight per day. The use of dihydroquercetin, blue-green algae spirulina and organic iodine in various combinations in feeding piglets contributed to an increase in the adaptive ability of the body, pathogenetic reactivity and correction of metabolic processes, which provided a higher realization of the genetic potential of pigs. The introduction of feed additives into the diet of pigs contributed to an increase in the bactericidal activity of blood serum in the first experimental group by 1%, in the second experimental group by 14,1%, compared with the control group. The number of white blood cells in piglets of the experimental groups was lower and red blood cells is higher compared to piglets in the control group, which was reflected in the hemoglobin content of blood, which was higher than piglets from the control group by 2% and 8,9%, respectively.

scholarly journals The effect of twinship on selected hematological and biochemical parameters in late pregnant ewes

2021 ◽  
Vol 77 (10) ◽  
pp. 6578-2021
Author(s):  
Yenilmez K. ◽  
Arslan S. ◽  
Kiliç S. ◽  
Atalay H.
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Ultrasound Examination ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Negative Energy ◽  
High Positive Correlation ◽  
Blood Samples ◽  
Positive Correlation ◽  
Hematological And Biochemical Parameters

This study aimed to determine the effect of twinship on the hematological and biochemical parameters in ewes of the Kıvırcık breed commonly raised in the Thrace region of Turkey. The study material consisted of 30 Kıvırcık ewes at days 130-150 of gestation, aged 3-5 years, weighing 55.61 ± 0.76 kg. After ultrasound examination, the ewes included in the study were divided into a single-pregnant group (n = 15) and a twinpregnant group (n = 15), and their blood samples were taken. The levels of red blood cells (RBC), hematocrit (HCT), hemoglobin (HGB) white blood cells (WBC), lymphocytes (LYM), monocytes (MON), non-esterified fatty acid (NEFA), β-hydroxybutyrate (BHBA), glucose, cholesterol, total protein, aspartate aminotransferase (AST), alanine aminotransferase (ALT) were determined in their blood samples. It was found that, although twinship did not affect hemogram values in ewes (p > 0.05), the biochemical parameters, BHBA, ALT, and AST values, were significantly affected by twinship (p <0.05). A high positive correlation was found between BHBA and NEFA. Thus it was concluded that twin pregnancy did not cause any significant changes in hematological parameters in Kıvırcık ewes. However, the significantly higher BHBA in twin-pregnant ewes and the high positive correlation between BHBA and NEFA indicate that the negative energy balance started to affect these animals, and a carbohydrate deficiency emerged. Therefore, twin-pregnant ewes should be monitored closely to reduce the risk of ketosis.

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