scholarly journals Impact of forest fire smoke on the state of the central nervous system of rats

2021 ◽  
Vol 100 (11) ◽  
pp. 1224-1228
Author(s):  
Larisa M. Sosedova ◽  
Vera A. Vokina ◽  
Mikhail A. Novikov ◽  
Elizaveta S. Andreeva ◽  
Viktor S. Rukavishnikov
Keyword(s):  
Forest Fire ◽  
Right Hemisphere ◽  
White Male ◽  
Experimental Studies ◽  
Smoke Inhalation ◽  
Male Rats ◽  
Control Group ◽  
Bioelectrical Activity ◽  
Average Amplitude ◽  
Fire Smoke

Introduction. The adverse negative effect of forest fire smoke on human health represents a unique interdisciplinary challenge to the scientific community. The influence of forest fire smoke on locomotor activity, cognitive indices, and brain bioelectrical activity parameters in exposed rats is presented. Materials and methods. Experimental studies were carried out on outbred white male rats. The animals of the experimental group were exposed to smoke inhalation forest fire for one day. Immediately after the end of the exposure, the animals were examined, including testing in an open field and Morris water maze, as well as an electroencephalographic examination. Results. At twenty-four-hour exposure to wildfire smoke in the model, conditions showed increasing motor and research activity of male rats against the backdrop of growing anxiety. Disorders of indicators of spatial memory and navigation learning were not revealed. On the encephalogram of the exposed animals, in comparison with the control group, the δ-rhythm range predominated, more pronounced in the leads of the right hemisphere. A decrease in the power spectrum and the average amplitude β1-rhythm, as well as a tendency to decrease the average amplitude of θ-rhythm, were revealed. The indices of the primary EEG rhythms did not have statistically significant differences when compared with the control group. Conclusion. The results showed that forest fire smoke leads to changes in the bioelectric activity of brain structures and dysregulation of individual behaviour in animals, all of which may indicate the formation of increased levels of stressing beyond physiological adaptation.

scholarly journals Experimental study of the gonadotoxic effect of forest fire smoke

2020 ◽  
Vol 99 (10) ◽  
pp. 1149-1152
Author(s):  
Mikhail A. Novikov ◽  
Vera A. Vokina ◽  
Elizaveta S. Andreeva ◽  
Anton N. Alekseenko ◽  
Larisa M. Sosedova
Keyword(s):  
Experimental Study ◽  
Forest Fire ◽  
Reproductive System ◽  
Leydig Cells ◽  
White Male ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Natural Fires ◽  
White Rats

Introduction. The study of the effects of air smoke during natural fires on the reproductive system is the actual scientific problem at present. Material and methods. The experimental study took place in 2 stages. At the first stage of the study, white male rats were exposed to the smoke-bearing effects of wildfire for 7 days (4 hours a day), and at the second stage, 5 days a week (4 hours a day) for one month. Immediately after the end of the exposure, the animals were decapitated under light ether anesthesia to study the morphofunctional state of the reproductive system, which included counting the total number of spermatogonia, the number of tubules with desquamated epithelium, as well as determining the spermatogenesis index and the number of Leydig cells. Results. The functional state of the reproductive system of male white rats after exposure to smoke for 1 week did not have statistically significant differences from the animals of the control group. At the same time, an increment in the duration of exposure (4 weeks) led to significant morphological and functional disorders of the reproductive system of male white rats, characterized by a decrease in the spermatogenesis index with a simultaneous decrease in the percentage of spermatogonia and the number of interstitial Leydig cells. Discussion. Male reproductive health can be a sensitive marker of environmental factors, and there is no evidence of smoke from natural fires in modern literature. This study justifies the need to develop new approaches to the diagnosis and prevention of adverse effects on the body for a long stay in a smoke area. Conclusion. The results of the study indicate the prolonged exposure to combustion products released during a forest fire to lead to inhibition of reproductive function in male white rats.

PROLONGED BAKTERIOPHAGAL INFECTION OF GUT MICROBIOTA REDUCES THE EXPRESSION OF ALPHA-SYNUCLEIN IN THE RAT PANETH CELLS

2020 ◽  
pp. 60-65
Author(s):  
Татьяна Николаевна Сергеева ◽  
Владимир Николаевич Николенко ◽  
Юлия Николаевна Кузнецова ◽  
Валерий Георгиевич Сергеев
Keyword(s):  
Gut Microbiota ◽  
Experimental Studies ◽  
Physiological Saline ◽  
Alpha Synuclein ◽  
Paneth Cells ◽  
Male Rats ◽  
Control Group ◽  
Human Pathogens ◽  
Bacteriophage Infection ◽  
Intestinal Dysbiosis

Цель - исследовать интенсивность экспрессии белка альфа-синуклеина клетками Панета у крыс в норме и условиях длительного бактериофагального инфицирования микробиоты. Материал и методы. Экспериментальные исследования проведены на 12 половозрелых крысах-самцах линии Вистар массой 280-320 г. Крысам основной группы (n=7) еженедельно на протяжении 12 нед ректально вводили 0,5 мл раствора, содержащего смесь бактериофагов против 14 патогенов человека (Microgen, Россия). Однократная доза вводимой бактериофагальной смеси содержала 0,5×10ед./мл каждого фага. Животные контрольной группы (n=5) получали по аналогичной схеме 0,5 мл стерильного изотонического раствора натрия хлорида. После интракардиальной перфузии отбирали фрагменты проксимального отдела подвздошной кишки на уровне 1-3 см выше илеоцекального соединения. Серийные криостатные срезы кишечника использовали для окрашивания гематоксилином - эозином и выявления иммунопозитивного альфа-синуклеина при помощи моноклональных мышиных антител (Sigma-Aldrich, USA) и коммерческого набора, содержащего авидин-биотин-пероксидазный комплекс (АBC Elite; Vector Laboratories, Burlingame, CA). Результаты. Длительное бактериофагальное инфицирование приводило к значимому снижению относительно контроля количества клеток, иммунопозитивных к альфа-синуклеину. В клетках Панета значимо снижались площади, занимаемые иммунореактивным продуктом к альфа-синуклеину и ацидофильными гранулами. Выводы. В апикальных частях клеток Панета, в области локализации ацидофильных гранул детектируется иммунопозитивный альфа-синуклеин. Дисбиоз кишечника, вызываемый бактериофагальным инфицированием микробиоты, приводит к гранулярному истощению клеток Панета и снижению экспрессии в них иммунореактивного альфа-синуклеина, что свидетельствует о его вовлеченности в механизмы экскреции. Objective - to investigate the intensity of the alpha - synuclein expression by Paneth cells of rat in normal conditions and prolonged bacteriophagal infection of gut microbiota. Material and methods. Experimental studies were performed on 12 adult Wistar male rats weighting 280-320 g. The rats of the main group (n=7) received rectally a 0,5 ml of solution containing a mixture of bacteriophages directed against 14 human pathogens (Microgen, Russia). The solution was introduced weekly for a period of 12 weeks. Each dose of bacteriophagal mixture contained 0,5×10 units/ml of each phage. Animals of the control group (n=5) received 0,5 ml of sterile physiological saline according to the same scheme. After transcardial perfusion, specimens of proximal portion of ileum 1-2 cm upstream the ileocecal junction were obtained. Serial cryostat sections were used for hematoxylin and eosin staining and for detection of immunopositive alpha-synuclein by monoclonal mouse antibodies (Sigma-Aldrich, USA) and commercially available kit containing avidin-biotin-peroxidase complex (АBC Elite; Vector Laboratories, Burlingame, CA). Results. Prolonged bacteriophage infection led to a significant decrease in the number of alpha-synuclein immunopositive cells compared with control. The area of Paneth cells occupied by the alpha synuclein-immunoreactive product and acidophilic granules significantly reduced. Conclusions. Immunopositive alpha-synuclein was detected in the apical parts of Paneth cells, in the area of acidophilic granules localization. The intestinal dysbiosis caused by bacteriophage infection of microbiota led to granular depletion of Paneth cells and a decrease in the expression of immunoreactive alphasynuclein in them, which indicates its involvement in excretion mechanisms.

Abstract WP288: Inter-Alpha Inhibitor Proteins Reduce the Presence of Neutrophils and Matrix Metalloproteinase-9 Positive Neutrophils in Damaged Brain Regions of Neonates with Hypoxic-Ischemic (HI) Brain Injury

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Adriel Barrios-Anderson ◽  
Xiaodi Chen ◽  
Yow-Pin Lim ◽  
Barbara S Stonestreet
Keyword(s):  
Brain Injury ◽  
Corpus Callosum ◽  
Matrix Metalloproteinase ◽  
Right Hemisphere ◽  
Brain Regions ◽  
Matrix Metalloproteinase 9 ◽  
Male Rats ◽  
Control Group ◽  
Neonatal Rats ◽  
Metalloproteinase 9

Introduction: Inter-alpha inhibitor proteins (IAIPs) are immunomodulatory proteins that play a significant anti-inflammatory role in hypoxic ischemic (HI) brain injury. We have shown that administering IAIPs after HI improves histopathological brain injury, brain weight, and behavioral outcomes in neonatal rats. Neutrophils are specialized leukocytes known to infiltrate the brain parenchyma and exacerbate neuronal injury after HI. One molecular mechanism by which neutrophils exert damage on the blood-brain barrier (BBB) and brain tissue after ischemia is by the release of matrix metalloproteinase-9 (MMP9), an enzyme that breaks down the extracellular matrices of surrounding cells. Objective: To determine the effect of IAIPs on neutrophil infiltration and release of MMP9 in neonatal rats after HI. Methods: The Vannucci model was used to induce neonatal HI in postnatal day 7 rats that were assigned to a Non-ischemic sham-control group (Sham, n=12), right-sided carotid ligation with exposure to hypoxia (8% oxygen for 90 min) treated with placebo group (PL-HI, n=17), or an IAIP treated group (IAIP-HI, n=17). Rat sex was recorded. IAIP (30 mg/kg) or PL was given intraperitoneally at 0, 24 and 48 h after HI. We removed the rat brain after 72h and performed immunohistochemistry using MPO (neutrophil selective) and MMP9 fluorescent markers. We performed stereological analyses with the StereoInvestigator 10.0 Fractionator probe without knowledge of group assignment to quantify neutrophils and MMP9 positive cells present within the right hemisphere, cortex, corpus callosum, and hippocampus. Results: MPO positive cells were significantly reduced in male IAIP treated rats compared with PL-HI in the overall damaged hemisphere (p<0.01) and the corpus callosum (p<0.05). Further, we observed MPO and MMP9 co-localization, and IAIP treatment reduced the presence of MMP9 positive neutrophils in the cortex of male rats compared to placebo (P<0.05).

scholarly journals Changes in the sizes of the kidney after contralateral nephrectomy in the experiment

2018 ◽  
Vol 24 (3) ◽  
pp. 52-57
Author(s):  
V.M. Monastyrskyi
Keyword(s):  
Postoperative Period ◽  
Left Kidney ◽  
Abdominal Cavity ◽  
White Male ◽  
Male Rats ◽  
Control Group ◽  
Close Relationship ◽  
Length Width ◽  
Sexually Mature ◽  
Experimental Group

The evaluation of renal measurements such as length, width and thickness, volume is important in the diagnosis and treatment of many renal disorders, since there is a close relationship between the sizes of the kidneys and its function. The purpose of the study was to establish and compare during the postoperative period changes in the mass and sizes of the kidney left after nephrectomy in the sexually mature male rats. An experimental study was carried out on 84 sexually mature white male rats weighing 178-194 grams. Animals were divided into two groups: control (42 rats) and experimental (42 rats). In the control group, the animals under ketamine anesthesia were followed by an abdominal cavity dilution, after which the abdominal wall was applied in a row. All animals of the experimental group performed surgical intervention – nephrectomy of the left kidney. The animals were withdrawn from the trial by intra-pleural administration of thiopental-sodium 50 mg/kg after 7, 14, 21, 30, 90 days after nephrectomy. Macroscopic evaluation and description of the kidneys of animals was performed after their removal. Their weight was determined on the laboratory scale of HLR-200 up to 0.1 mg, and the length, width and thickness of the organ were measured with the help of a caliper to an accuracy of 0.05 mm. Calculated the volume of the kidney. The statistical analysis of the obtained results was carried out using the program STATISTICA 5.5 using parametric methods for evaluating the results. It was established that the mass, length, width, thickness and volume of the kidney of animals in the experimental group, as compared with the control group, were statistically significantly higher in all terms of observation. The fraction of the growth of the width and thickness of the single kidney of animals in the experimental group compared with the control animals in the animals was statistically significantly greater than the proportion of kidney growth during the postoperative period. It was found that the largest increase in the mass and thickness of the kidney, as compared with the control group, was observed after 30 days of the postoperative period, and the greatest increase in the length and width of the kidney, as compared with the control group, was observed after 14 days of the postoperative period.

scholarly journals Protection of Dayak Onion Tuber Extract (Eleutherine Palmifolia) Against Kidney Histopathological Appearence of Albino Male Rat Strain Wistar which was Induced by Alloxan

2017 ◽  
Vol 3 (6) ◽  
pp. 702 ◽  
Author(s):  
Dwi Gayatri Nurcahyawati ◽  
Hani Plumeriastuti ◽  
Lilik Maslachah
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Oral Therapy ◽  
White Male ◽  
Male Rats ◽  
Male Rat ◽  
Glomerular Sclerosis ◽  
Control Group ◽  
Per Oral ◽  
Tuber Extract

The purpose of this study was to know the effect of Dayak onion tuber extract (Eleutherine palmifolia) given by per oral in lowering levels of histophatology damage kidney of albino male rat (Rattus norvegicus) strain Wistar which was induced by alloxan. Animals which were used in this research were 24 white male rats (Rattus norvegicus) strain Wistar, and then divided into 6 groups. The negative control group K (-) were given with aquabidest and CMC-Na 1% during the therapy period, the positive control group K (+) were given with alloxan 110 mg / kgbw, the group of drug control K (O) were given with alloxan and oral therapy  with metformin 9 mg / 200g bw / day, the treatment group 1 (P1) were given with alloxan and per oral therapy with extract of  Dayak onion tuber 100 mg / kgbw, the treatment group 2 (P2) were given with alloxan and per oral therapy with extract of Dayak onion tuber 200 mg / kgbw and the treatment group 3 (P3) were given with alloxan and per oral therapy with extract of Dayak onion tuber 400 mg / kgbw. The therapy was given for 14 days, then the animals were sacrificed with ketamine and then its kidney was taken for examination of hisphatology in kidney. Observations based on their depiction of renal histopathology tubular degeneration and necrosis, glomerular necrosis, intestitial infiltration and glomerular sclerosis. Data obtained from the scoring of histopathological appearence albino rat kidneys were analyzed by test Kruskal-Wallis and if there is a real difference followed by Mann-Whitney test using SPSS 20.0 for windows. The results showed that the extract of Dayak onion tuber 400 mg/ kgbw  can reduce the degree of kidney damage in albino male rat exposed to alloxan significantly.  Key words: Eleutherine palmifolia, alloxan, kidney, histhopathology

scholarly journals Features of morphological changes in the thyroid gland of white male rats 1 day after thermal trauma of the skin on the background of the introduction of 0.9 % NaCl solution

2019 ◽  
pp. 55-59
Author(s):  
O. I. Tiron
Keyword(s):  
Thyroid Gland ◽  
White Male ◽  
Experimental Studies ◽  
Vena Cava ◽  
Scientific Data ◽  
The Body ◽  
Male Rats ◽  
Nacl Solution ◽  
Experimental Animals ◽  
Thermal Trauma

Despite the fact that the main pathogenetic factor in the development of burn disease is the alteration of the skin, the severity of the condition of patients in the acute period of the disease is due to systemic changes in the body caused by thermal factor. Being a powerful stress factor, severe burns are accompanied by activation of the hypothalamic-pituitary system. Although thyroid hormones are not considered typical "stress hormones", they also play a role in maintaining vasomotor function under stress. The question of thyroid dysfunction on the background of burn injury remains debatable, and scientific data on its morphological and ultramicroscopic changes are quite limited. The aim of the work is to establish microscopic and submicroscopic changes of the thyroid gland of experimental animals in the stage of shock after thermal trauma of the skin. Experimental studies were performed on 12 white adult male rats, which received a thermal burn of the skin of 2-3 degrees with a lesion area of 21-23 % of the skin. An infusion of 0.9 % NaCl solution at a dose of 10 ml/kg was performed into the inferior vena cava. Collection of material for histological and electron microscopic examinations was performed according to generally accepted methods. Histological specimens were examined using a MIKROmed SEO SCAN light microscope and photo-documented using a Vision CCD Camera with an image output system from histological specimens. Ultrathin sections made on an ultramicrotome UMPT-7 were contrasted with uranyl acetate, lead citrate according to the Reynolds method and studied under an electron microscope PEM-125K. Conducted micro- and submicroscopic studies of the structural components of the thyroid gland a day after the reproduction of thermal trauma to the skin on the background of the introduction of 0.9 % NaCl solution revealed reactive adjunctive-compensatory changes and initial manifestations of destruction. Vascular, stromal and parenchymal components of the organ in the stage of shock after burns correspond to the state of "stress" as a reaction to pathological exogenous exposure, and the intensification of metabolic processes in the affected organism of experimental animals.

scholarly journals Pengaruh Pemberian Ekstrak Rumput Laut Cokelat (Sargassum duplicatum) terhadap Peningkatan Kadar Hematokrit pada Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Anemia yang di Induksi NaNO2

2020 ◽  
Vol 3 (2) ◽  
pp. 38
Author(s):  
Ayu Mayang A. P. ◽  
Liliawanti Liliawanti ◽  
Wachjudi Kurnia
Keyword(s):  
Rattus Norvegicus ◽  
White Male ◽  
Brown Seaweed ◽  
Negative Control ◽  
Laboratory Method ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference

<p>Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in the body. The components from brown seaweed such as cobalamin (vitamin B12), pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body. The purpose of this research was to determine the effect of brown seaweed (<em>Sargassum duplicatum</em>) which can increase hematocrit levels in the blood of white male wistar strain rat (<em>Rattus norvegicus</em>) suffered from anemia in induction NaNO<sub>2</sub>.</p><p>            This research uses an experimental laboratory method with a post test only control group design. The samples were 30 male rats divided into 3 treatment groups, the negative control group (K<sub>0</sub>) without treatment, the positive control (K<sub>1</sub>) be treated anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract and treatment group (K<sub>2</sub>) treated with anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract at a dose of 2,45gr / kgBB. On the 40<sup>th</sup> day, a blood sample was taken, then measured the hematocrit level.</p><p>            The results showed that the mean number of blood hematocrit levels in K<sub>2</sub> was higher than K<sub>1</sub>, it showed a significant difference descriptively. Based on the Kruskal-Wallis test, a value of 0.260 was obtained that p &gt; 0,05 showed that there were no statistically significant differences between groups of experimental animals.</p><p>            From the results of this research, the conclusion is the administration of brown seaweed extract (<em>Sargassum duplicatum</em>) descriptively increased, but not statistically significant.</p><p> </p><p><strong>Keywords :</strong> Anemia, hematocrit, <em>Sargassum duplicatum</em>, NaNO<sub>2</sub></p>

scholarly journals Enterosgel role in neurodegenerative changes in the retina rats under the influence of Cr (VI) - induced retinopathy by study morphological changes in dynamic

2021 ◽  
pp. 14-20
Author(s):  
Olena Kuzenko ◽  
Yevhen Kuzenko ◽  
Yuri Demin
Keyword(s):  
Drinking Water ◽  
Morphological Changes ◽  
White Male ◽  
Degenerative Changes ◽  
Male Rats ◽  
Control Group ◽  
Fiber Layer ◽  
Experimental Conditions ◽  
Typical Structure ◽  
Ganglion Neurons

Chromium galvanic production have leaded to biosphere pollution. Therefore advisable to study of role in the neurodegenerative development in retinal diseases under experimental conditions. The aim is to study the Enterosgel effect on morphological changes in rats retina with Cr(VI) – induced retinopathy. Materials and methods. An experimental study had carried out on 72 outbred white male rats. The rats had divided into 3 groups: I – control group of intact rats (n = 24). Control rats were received drinking water, II group – rats (n = 24), were received drinking water with Cr (VI) (K2Cr2O7) – 0.02 mol/L, III group – animals (n = 24) were received drinking water with K2Cr2O7– 0.02 mol/L and hydrogel methylsilicic acid (Enterosgel) at a dose of 0.8 mg/kg per day as a corrector. The animals had been decapitated under ether anesthesia. The retina had been studied on days 20, 40 and 60 of the experiment. Morphologically and morphometrically they had analyzed. Results. According to histological studies, it has proved that Cr (VI) causes dystrophic and degenerative changes in all rats retina layers. They increase as the duration of the experiment. The use of Enterosgel as a corrective therapy showed positive results in restoring the morphological structure of rats retina. After Enterosgel 20 days using as a corrector of Cr (VI) exposure, there is a barely noticeable swelling of the outer and inner nuclear layers. Other layers of the retina, morphologically, look undamaged. Forty days Enterosgel treatment have outer and inner nuclear layer edema of retina of animals persists but does not increase. It is easy noticeable swelling of the outer and inner layers of mesh, but no signs of damage processes of cell populations nuclear layers. State ganglionic layer and nerve fiber layer entirely satisfactory. These pathological changes are not critical. After 60 days from the beginning of loading of Cr (VI) and application of Enterosgel in the retina of rats there are initial degenerative changes in the photosensory layer. Cystic dilated outer segments of rods and cones were visible throughout, and areas of their fragmentation were observed. Ganglion neurons are not damaged, but their axons appear somewhat thickened and fluffy. But in general, the typical structure of the retina is preserved. Conclusions. Chromium-induced toxicity in rats is characterized by pronounced histological and morphometric changes and retinal thickness, which appear after 20 days, increase by 40 days and acquire maximum transformations after 60 days of the experiment. The use of Enterosgel improves picture morphological structures of the retina in rats under the influence of Cr (VI). The changes were expressed on days 20 and 40, which indicates the presence of protective properties for the retina

scholarly journals Manalagi Apple Vinegar (Malus sylvestris Mill) as Anti Diabetic to Alloxan Induced Wistar White Male Rat

2019 ◽  
Vol 2 (1) ◽  
pp. 44
Author(s):  
Valentina Verrel Purnomo ◽  
Agustinus Sareh Arjono Tjandra ◽  
Risma Risma
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
White Male ◽  
Test Method ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Standard Diet ◽  
Malus Sylvestris

<p>Apple vinegar is estimated to be able to cure diabetes. Vinegar is able to control the increase of glicemic index. Apple has antioxidant substance such as quercetin and chlorogenic acid. Manalagi apple could be found easily in Indonesia.</p><p>            This research aims to reveal the effect of Manalagi apple vinegar (<em>Malus sylvestris </em>Mill) towards blood glucose level of alloxan induced Wistar white male rats (<em>Rattus norvegicus</em>).</p><p>            This research is experimental laboratory using <em>Experimental </em>methods. The samples are 30 white rats divided into three groups for 31 days, 1) group which only given standard diet; 2) group that induced by 120mg/dL alloxan; 3) group that induced by 120mg/dL alloxan and Manalagi apple vinegar 0.27ml/100gram weight for 14 days. The measurement of blood serum glucose level is done on day twenty four using homogenous colorimetric enzymatic test method for all groups.</p><p>            Blood glucose average in group with only given standard diet as negative control group (182.33 mg/dL), group which induced by 120mg/dL alloxan as positive control group (205.2 mg/dL), group which induced by 120mg/dL alloxan and Manalagi apple vinegar as treatment group (212.25 mg/dL). The result of <em>One-Way Anova </em>significance<em> </em>is 0.229.</p><p>            The conclusion of this research is that Manalagi apple vinegar treatment doesn’t able to decrease the blood glucose level of white Wistar male rats significantly.</p><p> </p><strong>Keywords </strong>: <em>Malus sylvestris </em>Mill, Blood glucose, Alloxan.

Metformin Contributed with Lactic Acidosis in The White Male Rats

2019 ◽  
Vol 10 (04) ◽  
pp. 708-712
Author(s):  
Afyaa Sabah Nasir
Keyword(s):  
Lactic Acidosis ◽  
White Male ◽  
Clinical Problem ◽  
Male Rats ◽  
Control Group ◽  
Final Weight ◽  
Long Time ◽  
Liver And Kidney ◽  
Significant Change ◽  
Harmful Effects

Metformin treatment associated with development lactic acidosis (MALA) is a clinical problem. Recently, not found any drug to decrease or prevent MALA. The present study is designed to evaluate the advantage and disadvantages of metformin drugs in white male rats. A sample of 30 white male rats were randomly divided into three groups each group contain ten rats.: Group one administrated distal water to kept as control group for two months, Group two administrated metformin at dose 250 mg/kg for two months, and Group three administrated metformin at dose 500 mg/kg for two months. After the end of the experiment, two months, the rats were sacrificed to obtain the blood and tissues for analysis. The results show no significant change (p andgt; 0.05) in the final weight of rats and the weight of the kidney and liver relative to the bodyweight as well as, the results show no significant change (p andGLT; 0.05) in the levels of urea and creatinine in the serum of rats treated with metformin drug. Also, the results appear no significant change (p andgt; 0.05) in the liver enzymes include aspartate aminotransferase, alanine aminotransferase, alkaline aminotransferase, gamma-glutamyltransferase and total bilirubin in the metformin-treated groups relative to controls. In conclusion, the present study recorded not found harmful effects in the liver and kidney after taking metformin against diabetes except lactic acidosis state after using a drug for a long time.

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