Penetapan Kadar Senyawa β-Karoten Dalam Buah Semangka Kuning (Citrullus vulgaris Schrad) Dengan Metode Spektrofotometri UV-Vis

2021 ◽  
Vol 1 ◽  
pp. 351-357
Author(s):  
Maya Nisrina Huwaida ◽  
W Wirasti ◽  
S Slamet ◽  
Achmad Vandian Nur
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Test Results ◽  
Natural Pigment ◽  
Carotene Content ◽  
Citrullus Vulgaris ◽  
Good For ◽  
Β Carotene

AbstractWatermelon is a fruit that favored by the peoples because it tastes sweet and is good for health, watermelon also contains β-carotene. β-carotene is a natural pigment that is found in large amounts in red-orange fruits, including yellow watermelons. The purpose of this study was to determine β-carotene compounds and β-carotene levels in yellow watermelon flesh. The method used in this study for qualitative analysis using TLC method with chloroform: ethyl acetate (5: 5) mobile phase and for quantitative analysis using UV-Vis Spectrophotometry method. The test results showed that the yellow watermelon flesh sample contained β-carotene with a maximum wavelength of 458 nm and the β-carotene content of the yellow watermelon flesh was 146,253 µg/100 grams. Levels of β-carotene compounds in yellow watermelon flesh are included in the low category.Keywords: Yellow watermelon, β-carotene, TLC, UV-Vis spectrophotometry AbstrakSemangka merupakan buah yang banyak digemari oleh masyarakat karena rasanya manis dan baik bagi kesehatan, juga memiliki kandungan β-karoten. β-karoten adalah pigmen alami yang terdapat dalam jumlah besar pada buah-buahan yang berwarna merah-orange termasuk pada buah semangka kuning. Tujuan peneltian ini adalah untuk mengetahui senyawa β-karoten dan kadar β-karoten pada daging buah semangka kuning. Metode yang digunakan dalam penelitian ini untuk analisis kualitatif menggunakan metode KLT dengan fase gerak kloroform : etil asetat (5 : 5) dan untuk analisis kuantitatif menggunakan metode Spektrofotometri UV-Vis. Hasil pengujian menunjukkan sampel daging buah semangka kuning megandung β-karoten dengan Panjang gelombang maksimum yaitu 458 nm dengan kadar β-karoten pada daging buah semangka kuning yaitu 146,253 µg/100 gram. Kadar senyawa β-karoten dalam daging buah semangka kuning termasuk dalam kategori rendah.Kata kunci: Semangka kuning; β-karoten; KLT; spektrofotometri UV-Vis

scholarly journals Analisis Senyawa β-Karoten pada Buah Pepaya (Carica papaya L.) Asal Kabupaten Konawe Selatan, Provinsi Sulawesi Tenggara

2021 ◽  
Vol 4 (2) ◽  
pp. 1-7
Author(s):  
Irman Idrus ◽  
Sabda Wahab ◽  
Andi Fitrah Nugraha ◽  
Syaiful Bachri
Keyword(s):  
Quantitative Analysis ◽  
The Body ◽  
Test Results ◽  
Carotene Content ◽  
Test Parameter ◽  
Qualitative Test ◽  
Uv Visible ◽  
Planting Method ◽  
Β Carotene

South Konawe District at Southeast Sulawesi Province is a producer of agriculture such as Papaya fruit ranging from 1168 quintals each year. Papaya fruit contains β-carotene which plays an important role in the formation of vitamin A in the body. This research was conducted to determine the β-carotene content of papaya fruit by using variables planting method, fruitage, and fruit condition. The mashed fruit was extracted using n-hexane: acetone: ethanol (2: 1: 1) v/v, then separated from polar and non-polar solutions. Qualitative analysis was carried out using the Carr-Price method and quantitative analysis or determination of β-carotene levels using the UV-Visible spectrophotometric method λ = 452.0 nm. The qualitative test results showed that the papaya fruit in the papaya fruit obtained by cultivation and growing wild with each variable half-ripe, ripe, and not fresh papaya identified the presence of β-carotene. The results of quantitative analysis of β-carotene levels in cultivated papaya fruit were 1.76 μL, half-ripe 1.32 μL, and cultivated young papaya 1.22 μL. In wild ripe papaya 1.75 μL, wild half-ripe papaya 1.3 μL and wild young papaya 1.21 μL. There is an effect of each test parameter starting from the parameters of planting method, fruitage, and fruit condition on β-carotene content in papaya fruit.

scholarly journals “SUBSTITUSI TEPUNG Cucurbita moschata (Duch.) Poir TERHADAP KADAR β-KAROTEN DAN DAYA TERIMA CAKE LABU KUNING”

2021 ◽  
Vol 4 (1) ◽  
pp. 7-15
Author(s):  
Frederika Pellu Dimu ◽  
Andriani Rafael ◽  
Sonya T.M Nge
Keyword(s):  
Cucurbita Moschata ◽  
Test Results ◽  
Alternative Source ◽  
Carotene Content ◽  
Tukey Test ◽  
A Value ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Collection Methods ◽  
Β Carotene

ABSTRACTPumpkin (Cucurbita moschata (Duch.) Poir ) is one of the vegetables that is rich in β-carotene as a precursor of vitamin A and has a fairly high carbohydrate content. Pumpkin processing can be done to produce various kinds of products, one of which is cake. Cake is a baked dough and liquid dough made from flour, sugar, salt, ingredients, shortening, milk, eggs, and aroma enhancer. This study aims to determine the levels of β-carotene contained in pumpkin cake and to determine the acceptability of β-carotene in pumpkin cake. This research is an experimental research. The experimental design used was Completely Randomized Design (CRD) with 1 control 4 treatments and each treatment was repeated 3 times. The research phase consisted of making flour and making cake. Data collection methods include organoleptic tests and β-carotene tests. Data were analyzed using analysis of variance / ANOVA to determine differences in quality, and if there is an influence then proceed with the Tukey test. Organoleptic test results show that the best treatment of pumpkin cake in terms of color is A1 with a value of 3.88, in terms of aroma namely A0 with a value of 3.92, in terms of texture is A0 with a value of 4.28 and in terms of taste is A0 with value of 4.16. As for the β-carotene test, the treatment of pumpkin cake with the highest β-carotene content was found in A4 with an average of 0.262 and the lowest level was in A0 with an average of 0.018. Based on research data, pumpkin flour is worthy of being used as an alternative source of flour because the resulting cake meets SNI quality standards both in terms of color, taste, aroma, texture and β-carotene content.Keywords : Cake, Pumpkin, β-Carotene, Organoleptic

scholarly journals TLC determination of some flavanones in the buds of different genus Populus species and hybrids

2018 ◽  
Vol 68 (2) ◽  
pp. 199-210 ◽  
Author(s):  
Loretta Pobłocka-Olech ◽  
Piotr Migas ◽  
Mirosława Krauze-Baranowska
Keyword(s):  
Quantitative Analysis ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Mobile Phase ◽  
Flavonoid Aglycones ◽  
Evaluation Mode ◽  
Populus Species

Abstract Flavonoids in the buds of eight Populus species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid aglycones belonging to different groups, namely, flavones, flavonols, flavanones and flavanonols, previously described as constituents of poplar buds, was performed on silica gel plates using a hexane/ethyl acetate/formic acid (60:40:1.3, V/V/V) mixture as the mobile phase. Pinocembrin and pinostrobin were found in the majority of analyzed poplar buds. For quantitative analysis of both compounds, two TLC evaluation modes, densitometric and videodensitometric, were compared and the established methods were validated. Concentrations of flavanones in some extracts differed slightly or significantly due to the analyzed plant matrix complexity and the TLC evaluation mode applied. Poplar buds rich in flavanones originated from P. × canadensis ‘Robusta’ (1.82 and 2.23 g per 100 g, resp.) and P. balsamifera (1.17 and 2.24 g per 100 g, resp.).

scholarly journals OPTIMATION OF ETHANOL CONCENTRATION IN EXTRACTION OF EUGENOL FROM GALANGAL RHIZOME

2017 ◽  
Vol 10 (14) ◽  
pp. 18
Author(s):  
Adi Yugatama ◽  
Nur Wahida Ardiyati ◽  
Ira Yulianti
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Thin Layer ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Ethanol Concentration ◽  
Ethanol Extract ◽  
Standard Curve ◽  
Layer Chromatography ◽  
Curve Equation

   Objective: The main objective of this study was to determine the optimal ethanol concentration as solvent for extracting eugenol from galangal.Methods: Galangal rhizome extraction was conducted with kinetic maceration at 50°C with various ethanol concentration as solvent (0%, 30%, 50%, 70%, and 96%). A 1:10 ratio of rhizome and ethanol was applied. The extract was obtained, then its eugenol concentration was analyzed qualitatively and quantitatively by thin-layer chromatography-densitometer with n-hexane:ethyl acetate (4:1) by using mobile phase and silica gel 60 F254 as stationary phase and applying a wavelength of 283 nm.Results: The result of qualitative analysis showed that extract of 70% and 96% ethanol had a spot with Rf value 0.63, which was equal to the Rf value of standard eugenol. Standard curve equation for the 70% ethanol extract was y=89318x+656.07 (r=0.9993) and for the 96% ethanol extract was y=8658x+1743 (r=0.9999). The result of the quantitative analysis showed that the 70% and 96% ethanol extract contained 4.85% and 4.79% eugenol, respectively.Conclusion: Extraction of galangal rhizome in 70% and 96% ethanol was positively containing eugenol. Highest eugenol concentration (4.85%) was obtained from galangal rhizome extraction in 70% ethanol.

scholarly journals The Effect of Method and Ripening Duration on the Content of β-Carotene of Fe’i Banana (Musa troglodytarum L.)

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Fredy Leiwakabessy
Keyword(s):  
Nutritional Value ◽  
Statistical Tests ◽  
Test Results ◽  
Carotene Content ◽  
Significant Difference ◽  
Β Carotene

Research was conducted to determine the effect of the method and duration of ripening on the content of β-carotene of Fe’i banana, as well as determine the method and duration of ripening is effective in maintaining the nutritional value of Fe’i Banana. Through laboratory experimental with randomized complete design consisting of 19 groups of treatments and three repetitions, the data obtained were analyzed using statistical tests ANOVA and continued with Duncan test. Results of the analysis showed that method ofripening statistically significant difference (p <0,05) on the content of β-carotene in the banana sticks sky. Long ripening length statistically significant difference (p <0, 05) on the content of β-carotene in the Fe’i Banana. Grilled ripening method is more effective against β-carotene content in the Fe’i Banana and takes1 0 minutes of ripening length more effectively on the content of β-carotene on a Fe’i Banana.

HPLC-UV Method for Determination of Famotidine from Pharmaceutical Products

2018 ◽  
Vol 69 (2) ◽  
pp. 297-299
Author(s):  
Adriana Nita ◽  
Delia Mirela Tit ◽  
Lucian Copolovici ◽  
Carmen Elena Melinte (Frunzulica) ◽  
Dana Maria Copolovici ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Quantitative Analysis ◽  
Linear Response ◽  
Mobile Phase ◽  
Acetic Acid Solution ◽  
Pharmaceutical Products ◽  
Quantification Limit ◽  
Validation Parameters ◽  
Hplc Analyses

The aim of this study was to develop and validate a rapid, accurate, and exact method for the quantitative determination of famotidine in pharmaceutical products. The HPLC analyses were performed by using a mobile phase containing methanol:1% acetic acid solution=30:7 (v/v), at a flow rate of 0.4 mL/min.The total time of the method was 10 min, and the retention time of famotidine was 4.16 min. The detection was evaluated at l=267 nm. The method has been validated by using different validation parameters. The linear response of the detector for famotidine peak area was observed at concentrations ranging from 0.1 to 0.0001 mg mL-1 , resulting in a correlation coefficient of 0.99998. The values of the detection limit and of the quantification limit are 0.00048 mg mL-1 and 0.00148 mg mL-1, respectively. The method proposed allowed accurate (with a relative error of less than 2%) and precise (RSD values less than 2.0%) determination of famotidine content in pharmaceutical products and can be used for its rapid quantitative analysis.

Exploring the Pharmacognostic Features, Anti-oxidant and Lipid Lowering Potential of Fagopyrum esculentum Moench. Seed

2020 ◽  
Vol 6 (2) ◽  
pp. 155-169
Author(s):  
Neeraj Panihar ◽  
Neeru Vasudeva ◽  
Sunil Sharma ◽  
Babu Lal Jangir
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Water Soluble ◽  
Fagopyrum Esculentum ◽  
Lipid Lowering ◽  
Standard Drug ◽  
Fagopyrum Esculentum Moench ◽  
Β Carotene

Background: Fagopyrum esculentum Moench. is a herb consumed as food and has medicinal value. It is a rich source of bioactive nutrients which cure and prevent many ailments. Traditionally, it is used to treat hypertension, diabetes, constipation, cancer etc. Methods and Objective: Present work illustrates morphological, microscopic and physicochemical parameters of Fagopyrum esculentum seeds as per WHO guidelines, in vitro antioxidant activity; assessed by DPPH scavenging method, hydrogen peroxide scavenging assay and β-carotene linoleic acid bleaching method and study of lipid lowering potential of the ethyl acetate and ethanol extract of seeds on normal diet fed Wistar rats. Results: Morphological studies delineated the triangular shape, dark brown colour, 8 mm length and 6 mm width of the seed. The microscopic examination of the transverse section of seed depicted features like testa or pericarp (seed coat), the endosperm, embryo and sclerenchyma cells. Study of physiochemical parameters exhibited 0.3±0.02% of foreign matter and 1.44±0.51% crude fibre content. Total ash, acid insoluble ash and water soluble ash value were 6.7±1.7%, 1.9±0.23% and 3.9± 0.31% respectively. Alcohol soluble and water soluble extractive value came out to be 65.02± 3.21 mg/g and 12.7±1.24 mg/g respectively. Foaming index was less than 100, swelling index was found to be 0.5±0.01 ml/g. Loss on drying was 4.02±1.27%. Phytochemical screening of ethyl acetate and ethanol extract revealed the presence of alkaloids, carbohydrates, phenolic compounds, phytosterols and flavonoids. Trace amount of heavy metals (arsenic, cadmium, lead, mercury) were determined by atomic absorption spectrophotometer. Pesticide residue analysis confirmed the presence of nontoxic pesticides like dimethipin, hymexazol, phenothrin-2, methoprene, triadimenol, prohydrojasmon- 1, jasmolin ii, triademinol, jasmolin i, prohydrojasmone i, cyromazine in both the extracts by gc-ms spectrometer. The ethyl acetate and ethanol extract has shown significant in-vitro antioxidant activities demonstrated by the DPPH method (IC50 = 94.37±2.51 and 216.04±4.39 μg/ml respectively), hydrogen peroxide scavenging assay (IC50 = 83.72±3.72 and 193.47±5.05 µg/ml respectively) and β-carotene bleaching method (IC50 = 100.67±4.01 and 205.39±2.89 µg/ml respectively). Lipid lowering study performed on Wistar rats demonstrated a significant (p<0.001) decrease in serum Total Cholesterol (TC), Triglyceride (TG) and increase in High Density Lipoprotein (HDL) level as compared to normal group. Both the extracts have shown a non significant difference in the level of TG as compared to standard drug atorvastatin, depicting that the efficacy of extracts is at par with that of standard drug atorvastatin. Conclusion: Pharmacognostical study of the plant can be a very good tool for identification as well as authentication of a herb. Moreover, these parameters may be helpful in the development of monograph of the plant. Pharmacological activity confirmed Fagopyrum esculentum Moench. seed to be a good antioxidant and have lipid lowering potential.

scholarly journals Intersexual Partial Diploids of Phycomyces

Genetics ◽  
2001 ◽  
Vol 158 (2) ◽  
pp. 635-641
Author(s):  
Bina J Mehta ◽  
Enrique Cerdá-Olmedo
Keyword(s):  
Dry Mass ◽  
Sexual Cycle ◽  
Genetic Constitution ◽  
Phycomyces Blakesleeanus ◽  
Carotene Content ◽  
Sexual Interaction ◽  
Sexual Stimulation ◽  
Nuclear Composition ◽  
Opposite Sex ◽  
Β Carotene

Abstract Sexual interaction between strains of opposite sex in many fungi of the order Mucorales modifies hyphal morphology and increases the carotene content. The progeny of crosses of Phycomyces blakesleeanus usually include a small proportion of anomalous segregants that show these signs of sexual stimulation without a partner. We have analyzed the genetic constitution of such segregants from crosses that involved a carF mutation for overaccumulation of β-carotene and other markers. The new strains were diploids or partial diploids heterozygous for the sex markers. Diploidy was unknown in this fungus and in the Zygomycetes. Random chromosome losses during the vegetative growth of the diploid led to heterokaryosis in the coenocytic mycelia and eventually to sectors of various tints and mating behavior. The changes in the nuclear composition of the mycelia could be followed by selecting for individual nuclei. The results impose a reinterpretation of the sexual cycle of Phycomyces. Some of the intersexual strains that carried the carF mutation contained 25 mg β-carotene per gram of dry mass and were sufficiently stable for practical use in carotene production.

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