EFFECT OF CITRULLUS LANATUS SEED'S (ETHANOLIC EXTRACT) ON NORMAL RENAL FUNCTIONS AND SERUM ELECTROLYTES OF A ALBINO RATS

2019 ◽  
Vol 01 (02) ◽  
Keyword(s):  
Citrullus Lanatus ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Serum Electrolytes ◽  
Renal Functions

scholarly journals Evaluating the influence of Citrullus lanatus seed extracts on electrolytes, urea and creatinine in Streptozotocin induced diabetic albino rats

2018 ◽  
pp. 87-94
Keyword(s):  
Sodium Chloride ◽  
Citrullus Lanatus ◽  
Colorimetric Method ◽  
Chloride Concentration ◽  
Ethanolic Extract ◽  
Sodium Concentration ◽  
Diabetic Control ◽  
Albino Rats ◽  
Potassium Sodium ◽  
Group 1

The focus of current research study was to evaluate the influence of the seed extract of Citrullus lanatus on urea, creatinine, potassium, sodium, chloride and bicarbonate in streptozotocin induced diabetic rats. Eighteen male wistar albino rats were divided into six groups of three rats each, diabetes were induced in all the rats except group 1 by intraperitoneal injection of 45 mg/kg b.wt. of streptozotocin. Group 1 rats served as control and received standard feed and water daily; Group 2 rats received oral Glibenclamide (0.5 mg/kg bw); Group 3,4, and 5 received 200 mg/kg bw, 400 mg/kg bw and 600 mg/kg b.wt. of the ethanolic extract of Citrullus lanatus seed respectively; and group 6 served as diabetic group. Blood samples were collected and analysed for urea, creatinine, potassium, sodium, chloride and bicarbonate using urease-Berthelot colorimetric method, Jaffe’s colorimetric method and Ion selective electrode (ISE) method respectively. There was a significant decrease in (p<0.05) glucose concentration (mmol/L) of 3.90 in control compared to 8.07, 8.73, 14.67, 11.43 and 9.80 in albino rats treated with glibenclamide, STZ+CLS 200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively. In potassium concentration (meq/l) 6.51 in control compared with 4.97, 7.59, 7.28, 8.45 and 6.87 in albino rats treated with glibenclamide, STZ+CLS200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively. While in sodium concentration (meq/l) of 1.42 in control compared to 1.33, 1.30, 1.36, 1.42 and 1.36 in albino rats treated with glibenclamide, STZ+CLS 200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively. However, in chloride concentration (meq/l) of 1.06 in control compared to 99.10, 97.87, 1.04, 88.00, 1.04, 88.00 and 1.04 in albino rats treated with glibenclamide, STZ+CLS 200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively and also in bicarbonate concentration (meq/l) of 10.07 when compared with 17.30, 16.47, 15.40, 6.40 and 17.30 in albino rats treated with glibenclamide, STZ+CLS 200 mg, STZ+CLS 400 mg, STZ+CLS 600 mg and diabetic control respectively. In conclusion, this medicinal plant could be considered as a potential and alternative approach for the treatment of diabetes. Keywords: Keywords: Citrullus lanatus, Diabetes Mellitus, Renal

scholarly journals Evaluation of the Cardioprotective Effect of Citrullus lanatus (Watermelon) Seeds in Streptozotocin Induced Diabetic Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
C. O. L. Karikpo ◽  
E. S. Bartimaeus ◽  
B. Holy
Keyword(s):  
Creatine Kinase ◽  
Body Weight ◽  
Lactate Dehydrogenase ◽  
Structural Integrity ◽  
Citrullus Lanatus ◽  
Ethanolic Extract ◽  
Control Group ◽  
Albino Rats ◽  
Duration Of Treatment ◽  
Glucose Levels

The study examined the cardioprotective potential of the ethanolic extract of Citrullus lanatus in streptozotocin induced diabetic albino rats. A total of sixty albino rats weighing approximately ± 200 g were used. The induction of diabetes in the rats was done using 50 mg/kg body weight of streptozotocin and confirmed by checking glucose levels using a glucometer. Albino rats with glucose levels greater than ≥ 250 mg/dl were considered diabetic. The rats were divided into 5 groups of 12 animals each and allowed access to food and water ad libitum. The animals had a 12 hour overnight fast after which diabetes was induced by injecting them intraperitoneally with streptozotocin (50 mg/kg). The control (group A) was not induced while diabetic control was induced. Induced diabetic rats in groups C, D and E were later treated with C. lantus at a dose of 100 mg/kg, 400 mg/kg, 800 mg/kg body weight respectively for 14 and 28 days respectively. After 14 days, 6 rats in each of the groups were sacrificed while the remaining 6 were sacrificed after 28 days. Blood samples were collected into lithium heparin bottles and used for the assay of cardiac enzymes (creatine kinase and lactate dehydrogenase) using standard procedures. The data obtained were compared using one-way analysis of variance (ANOVA) and the difference between means was obtained using Tukey's multiple tests of comparison. Analyses showed that the levels of creatine kinase (CK-MB) and lactate dehydrogenase(LDH) in the albino rats treated with C. lanatus were significantly (p<0.05) reduced when compared with the diabetic-induced group without treatment and the decrease was dose-dependent. A significant decrease (p<0.05) in the levels of the enzymes was also observed based on the duration of treatment with the extract. Since the enzymes are markers of cardiac injury, the decrease in their activity following treatment with C. lanatus shows that the extract possesses the significant cardioprotective potential of ameliorating structural integrity of the cardiac muscle in diabetic condition.

scholarly journals Comparative Study of the Effects of Ethanolic and n-Hexane Extracts of Garcinia kola Seeds on the Serum Electrolyte of Albino Rats

2020 ◽  
pp. 1-9
Author(s):  
I. R. Jack ◽  
N. Nwachoko ◽  
U. H. Nwafor ◽  
G. I. Ndukwe
Keyword(s):  
Biochemical Analysis ◽  
Ethanolic Extract ◽  
Proximate Analysis ◽  
Hexane Extract ◽  
Albino Rats ◽  
Serum Electrolytes ◽  
Garcinia Kola ◽  
Percent Composition ◽  
Hexane Extracts ◽  
Group 1

Garcinia kola (bitter kola) plays an important role in Africa ethnomedicine and traditional hospitality. Proximate and phytochemical composition of G. kola seeds as well as the effects of ethanolic and n-hexane extracts on the serum electrolytes of albino rats were studied using standard methods. Thirty-six albino rats of both sexes were used for the experiment. The animals were divided into nine groups of four rats per group. The groups were designated 1-9. Group 1 served as the control which was treated with normal saline. Groups 2-5 served as the groups treated with ethanolic extract of G. kola seeds and received 50, 125, 250 and 500 mg/kg body weight, while groups 6-9 served as the groups treated with n-hexane extract. After three weeks of treatment, the animals were sacrificed, and blood samples analyzed. Result of the proximate analysis showed that carbohydrate content was the highest (78.06%) while ash was the lowest (0.70%). Phytochemical result of G. kola seeds showed that tannins (0.342%) was the highest in terms of percent composition, followed by flavonoids (0.00764%); while alkaloids (0.00075%) was the lowest. Also, biochemical analysis revealed that the n-hexane extract of G. kola seeds was found to have slightly increased the activities of the serum electrolytes than the ethanolic extract. Conclusively, the results of this study showed that both extracts had effect on serum electrolytes of the albino rats, but the n-hexane extract had more toxic effect.

scholarly journals Protective Effect of Ethanolic Extract ofTabernaemontana divaricata(L.) R. Br. against DEN and Fe NTA Induced Liver Necrosis in Wistar Albino Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Kannappan Poornima ◽  
Palanisamy Chella Perumal ◽  
Velliyur Kanniappan Gopalakrishnan
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Protective Effects ◽  
Liver Necrosis ◽  
Standard Drug ◽  
Tabernaemontana Divaricata ◽  
Wistar Albino Rats

This study is an attempt to evaluate the hepatoprotective activity ofTabernaemontana divaricataagainst DEN and Fe NTA induced liver necrosis in rats. Ethanolic extract of the whole plant ofTabernaemontana divaricataat doses of 200 and 400 mg/kg body weight and 5-fluorouracil (standard drug) was orally administered to male Wistar Albino rats once daily for 24 weeks, simultaneously treated with the carcinogen DEN and Fe NTA. In simultaneously treated animals, the plant extract significantly decreased the levels of uric acid, bilirubin, AST, ALT, and ALP in serum and increased the levels of liver marker enzymes in liver. Treatment with the extracts resulted in a significant increase in the levels of antioxidants accompanied by a marked reduction in the levels of malondialdehyde when compared to DEN and Fe NTA treated group. When compared with 200 mg/kg bw rats, 400 mg/kg bw rats and 5-fluorouracil treated rats showed better results in all the parameters. The histopathological studies confirmed the protective effects of extract against DEN and Fe NTA induced liver necrosis. Thus, it could be concluded that the use ofTabernaemontana divaricataextract in the treatment of carcinogen induced hepatic necrosis.

Hepatoprotective Role of Propolis Extract to Prevent Hepatotoxic Effects of ATT in patients with Tuberculosis

2021 ◽  
Vol 15 (9) ◽  
pp. 2867-2869
Author(s):  
Asma Arshad ◽  
Saira Munawar ◽  
Rabia Sajjad Toor ◽  
Saba Saleem ◽  
Kanwal Sharif ◽  
...  
Keyword(s):  
Standard Dose ◽  
Ethanolic Extract ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Average Weight ◽  
Propolis Extract ◽  
Measured Weight ◽  
Group B ◽  
Group D

Objective: The main purpose of this study is to evaluate the hepatoprotective effects of propolis in hepatocytes injury caused by ATT due to isoniazid and rifampicin. Methods: Healthy albino rats of with average weight of 200-250g were under this study. These rats dividing into main four groups, A group is taken a control group and then further into the group B, group C, and group D as group for experiments. The control group had 15 rats with measured weight, they were given distilled water. Group B had 15 rats, they were given with standard dose of rifampicin and isoniazid. Group c had 15 rats, they were also given with standard dose of rifampicin and isoniazid. Group D had 15 rats, they were given with standard dose of rifampicin and isoniazid and also extract of the propolis we prepared. Results: Serum ALT in the experimental group B with group C, group D were also found to be of statistically significant with p-value < 0.001. ALT serum level observed high in group B. Multiple comparison between groups revealed that group B with a significantly increase in the serum enzyme AST level in comparison to group A, group C and group D with p-value <0.001. Conclusion: This study showed that ethanolic extract of propolis prevents isoniazid and rifampicin induced hepatotoxicity in the albino rats. Key words; Propolis, Anti-tuberculosis treatment, Hepato-toxicity.

scholarly journals Antihyperglycemic Activity of The Ethanolic Extract of the Bark of Mitragyna Ciliata (MYCA) in Albino Induced Diabetic Rats

2021 ◽  
pp. 957-965
Author(s):  
PAYE Plenseh Diana ◽  
James McClain
Keyword(s):  
Glucose Level ◽  
Hematological Parameters ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Vascular Complication ◽  
Severe Side Effect ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Toxicological Effect ◽  
Treatment Of Diabetes

Background: Diabetes an epidemic that gives rise to an increased macro vascular complication mortality rate of 1.5 million annually, increasing by 2035. Treatment of diabetes is complicated and associated with a severe side effect.</p> <p>Objective: To evaluate the antihyperglycemic property of the ethanolic extract of the back of MYCA and its toxicological effect on the hematological parameters with histopathological analysis of selected organs of induced diabetic albino rats.</p> <p>Method: The ethanoic extract (1000 mg/kg bw and 500mg/kg bw) and glibenclamide (0.5mg/kg bw) were administered to diabetic induced rats.  The effect of the extract on the hematological was studied in the diabetic induced rats. Histopathological changes were observed in the liver of induced diabetic rats after the administration of the MYCA extract.</p> <p>Results: The anti-hyperglycaemic effect was observed at two different levels, for two different doses (1000mg/kg and 500mg/kg). The results also showed significant suppression of blood glucose level in glucose fed hyperglycaemic albino rats but showed no significantly suppressed glucose level in overnight fasted normoglycemic rats.</p> <p>Conclusion: The findings revealed that the ethanolic extract of MITRAGYNA CILIATA (MYCA) possess antihyperglycemic property. Besides, the extract can prevent the various complication of diabetes and improve some hematological parameters.

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