scholarly journals Biological and functional properties of vine leaves

Author(s):  
Adiba Benahmed Djilali ◽  
Abdelouahab Benseddik ◽  
Halima Boughellout ◽  
Karim Allaf ◽  
Mohamed Nabiev

Aims: The main objective of the present research work was to evaluate the (phytochemical, biochemical, and antimicrobial) properties of Muscat of Alexandria leaf powder and develop new functional dairy product using the mixture lactic bacteria and vine leaf powder as prebiotic for health applications (gastric and cardiac problems, etc.). Material and Methods: Various nutritional parameters of the vine leaf powder namely: pH, acidity, water content, ashes, salts, fatty acids) were determined. Also, their bioactive substances (TPC, total flavonoids content, tannin content, soluble-water polysaccharides) were extracted and quantified using referenced methods. The evaluation of antimicrobial activity of these substances was carried out by disc method. Vine leaf powder and aqueous extract were used to improve acidification kinetic. Also, functional yogurt using the mixture (lactic bacteria and vine leaf powder as prebiotic) was prepared. Results: The main results demonstrate that, the vine leaf powder contains high-value components such as salts with a high k/Na ratio, fatty acids (palmitic, linolenic and oleic) and bioactives (polyphenols, tannins and polysaccharides). The antimicrobial activity of these bioactive metabolites varies depending on the resistance of the strains tested. On the other hand, vine leaf TPC and polysaccharides act as an antifungal against (C. albicans and A. niger) and increase the acidification rate and consequently the growth and activity of the lactic bacteria in the yogurt, which suggests a probable prebiotic effect. Conclusions: Through this study, we have demonstrated the high content of vine leaves in several bioactive compounds such as polyphenols, flavonoids, tannins and polysaccharides. These compounds display an interesting antimicrobial activity and an extensive effect on the activity of lactic bacteria, which suggests a prebiotic effect. Keywords: Bioactive substances, antimicrobial activity, prebiotic, vine leaves.

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Esther Ramírez-Moreno ◽  
Raquel Cariño-Cortés ◽  
Nelly del Socorro Cruz-Cansino ◽  
Luis Delgado-Olivares ◽  
José Alberto Ariza-Ortega ◽  
...  

Seed oils from two Mexican varieties of cactus pear (green: Opuntia albicarpa and red: Opuntia ficus indica) were extracted with different solvents (hexane, ethanol, and ethyl acetate) to evaluate their antioxidant activity. The seed oil with higher antioxidant activity was selected to evaluate antimicrobial activity. The fatty acid profile was analyzed by gas chromatography-mass spectrometry (GC-MS). Oil from green cactus pear seeds obtained with ethanol and ethyl acetate exhibited higher antioxidant activity (p<0.05) of 323 and 316 μmol TE/20 mg (p < 0.05), respectively, compared to red cactus pear seed oil (≈274 and 247 μmol TE/20 mg with ethyl acetate and ethanol, resp.). The oil obtained with ethanol and higher antioxidant activity was used to determine the antimicrobial activity. Both cactus pear oils produced a microbial inhibition zone in most of the microorganisms evaluated, particularly Saccharomyces cerevisiae which had similar diameter (38–40 mm). The oil fatty acids profiles of both varieties were similar and exhibited a high content of linoleic acid, while two fatty acids (linolenic and behenic) found in red cactus pear were not observed in the green variety.


Author(s):  
Monika Maheshwari ◽  
Anju Goyal

  Objective: The objective of this work was to synthesize and evaluate antimicrobial properties of 1-(4-methyl-6-nitro-2H-benzo[b][1,4]thiazine- 3(4H)-ylidene)hydrazine-1,1-dioxide derivatives.Methods: These new compounds were synthesized by methylation in 4-N and reacted with hydrazine derivatives and oxidized at the sulfur atom by 30% hydrogen peroxide to obtain sulfones. All the synthesized compounds were evaluated for antimicrobial activity using the disc diffusion method.Results: The Fourier transform infrared, 1H nuclear magnetic resonance (NMR), 13CNMR, and mass studies confirm the synthesis of some new 1-(4-methyl- 6-nitro-2H-benzo[b][1,4]thiazine-3(4H)-ylidene)hydrazine-1,1-dioxide derivatives. Compound 6f showed the potent antimicrobial activity.Conclusion: Result obtained in this research work clearly indicated that the compound 6f having methyl at 2 position and nitro groups at 2′ and 4′ position showed the most potent antimicrobial activity.


2016 ◽  
Vol 848 ◽  
pp. 123-126
Author(s):  
Noppakun Sanpo ◽  
Jirasak Tharajak

This research work offers the new organic compound which has potential to replace the use of tributylin (TBT) in antifouling application. All modified chitosan powders were synthesized using in-house powder processing techniques. Pure and modified chitosan were evaluated for fouling properties via antimicrobial activity test against Cobetia Marina (C. Marina). The results indicated that copper substituted chitosan (CS-Cu) showed the highest inhibition zone and lowest number of C. Marina survival. It can be implied that CS-Cu showed the best antimicrobial ability against C. Marina.


Catalysts ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 510 ◽  
Author(s):  
Wojciech Snoch ◽  
Karolina Stępień ◽  
Justyna Prajsnar ◽  
Jakub Staroń ◽  
Maciej Szaleniec ◽  
...  

Sugar esters are bioactive compounds derived from renewable resources. They consist of a sugar moiety with attached non-polar part – usually a fatty acid. These compounds find uses in cosmetic, food and pharmaceutical industries as surfactants due to their physicochemical and antimicrobial activities. In this study we have produced fatty acids for sugar ester synthesis from bacterially derived polyesters, namely polyhydroxyalkanoates (PHAs). We have developed methodology to decorate PHA monomers with a fluorinated moiety. With aid of biocatalysis a series of glucose esters was created with unmodified and modified PHA monomers. All synthesised compounds showed moderate antimicrobial activity.


2017 ◽  
Vol II (I) ◽  
pp. 1-9
Author(s):  
Mubashar Rehmana ◽  
Adeel Arshad ◽  
Muhammad Asadullah Madni

Free fatty acids possess antimicrobial properties in the natural defense system of many eukaryotes. In this study, we synthesized nanoformulations of myristic acid for antimicrobial activity. The myristic acid nanoformulations (MN) were unstable due to crystallization. This problem was overcome by the addition of the liquid fatty acids and by using two surfactants of hydrophilic and lipophilic nature. MN exhibited a small size (100 nm), and their physical form was affected by the amount of the liquid fatty acid. MN with myristic acid and liquid fatty acid in 1:1 formed solid NLC and 2:3 formed liquid nanoemulsions. In antimicrobial studies, MN was effective against the Gram- positive bacteria, i.e. Staphylococcus aureus and Bacillus subtillis, and the Gram-negative bacteria, i.e. Pseudomonas eruginosa and Salmonella typhi. MN8 (Myristic acid and oleic acid in 2:3) showed the highest antimicrobial activity and should be explored as potential antimicrobial agents against more strains of microorganisms.


2021 ◽  
Vol 10 (14) ◽  
pp. e113101421646
Author(s):  
Sandro Pinheiro da Costa ◽  
Raphaela Aparecida Schuenck-Rodrigues ◽  
Verônica da Silva Cardoso ◽  
Simone Sacramento Valverde ◽  
Alane Beatriz Vermelho ◽  
...  

Natural products produced from endophyte fungi have a broad spectrum of biological activity and can be grouped into several categories. Antimicrobials constitute an important group of therapeutic agents, which can be produced and obtained from living organisms. The main objective of this study is to investigate the endophytic fungi and the leaves of Brugmansia suaveolens Bercht. & J. Presl as elicitors, aiming at the production of bioactive substances with antimicrobial properties. The extracts were prepared from 19 fungi isolated from the leaves of B. suaveolens Bercht. & J. Presl, used as a biotic elicitor. The extracts were obtained by fermentation in a submerged medium with the addition of leaves from its dry leaves, and the antimicrobial activity of its extracts was evaluated using the agar diffusion method. The results showed that 57% of the endophytic fungi showed antimicrobial activity after fermentation with the elicitor. It was observed that the antimicrobial activity was increased compared to control cultures. The leaves of B. suaveolens Bercht. & J. Presl presented bioactives that stimulated the production of a substance with antimicrobial activity by endophytic fungi, the study of such compounds showed promise to clarify the application potential of these bioactives.


2020 ◽  
Vol 10 (6) ◽  
pp. 6991-7001

Nowadays using natural sources for treating disease is one of the most favorable methods because of fewer side effects. Reporting antimicrobial properties of a new natural source can be useful for designing new natural medicines antimicrobial effect of Nigella Sativa L. seeds n-butanol extract will be useful for treating or preventing for many infections. In our study we report the first GC-MS analysis of n-butanol extract of Nigella Sativa L. seed and also its antimicrobial activity against some food poisoning and nosocomial infection causing microorganisms. Nigella Sativa L. seed powder was subjected to n-butanol with ratio 1:10 in a flask and then kept on shaker. After filtering, the extract has been analyzed by GC-MS. After that, the extract was tested against bacteria and fungi by three method, disc diffusion, well diffusion, and micro dilution methods. The GC-MS analysis revealed that fatty acids and terpenoids are the major constituents. The result showed that pseudomonas aureoginosa, klebsiella pneumoniae, acinetobacter baumanii, Yersinia enterocolitica, Candida albicans, Candida parapsilosis and Candida krusei were the sensitive microorganisms. According to great antimicrobial activity and having nourishing components (fatty acids and terpenoids) adding this plant to a daily food diet is a good way for having a healthy life and this plant can be a new source for therapeutic uses.


2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.


2021 ◽  
pp. 1-30
Author(s):  
Raphaël Chouinard-Watkins ◽  
Melissa Calleja ◽  
Richard P Bazinet ◽  
Andrea R Josse

Abstract Dairy fat is rich in saturated fatty acids such as palmitic acid (16:0) but low in linoleic acid (18:2n-6). The natural carbon 13 enrichment (δ13C) of 16:0 is higher in dairy fat than in most of the food supply. In adults, serum levels of pentadecanoic acid (15:0) and heptadecanoic acid (17:0) are recognized as biomarkers of dairy intake. In adolescents, no study has evaluated serum fatty acid levels or δ13C in response to chronic dairy consumption. The objectives of this study were to evaluate whether increased dairy product consumption can modulate 1) serum fatty acid levels and 2) 16:0 δ13C in adolescents with overweight/obesity who followed a 12-week weight management program. This secondary analysis of a RCT included two groups of adolescent females: recommended dairy (RDa; n=23) and low dairy (LDa; n=23). The RDa group was given 4 servings/d of dairy products while the LDa group maintained dairy intakes at ≤2 servings/d. Blood was sampled before and after the intervention. Lipids were extracted, separated, and fatty acids were quantified by gas chromatography. Isotope ratio mass spectrometry was used to assess 16:0 δ13C. There were no group differences on serum changes of 15:0 or 17:0. Within triglycerides, 18:2n-6 was lowered by 7.4% only in the RDa group (p = 0.040). The difference in delta 16:0 δ13C between the LDa and RDa group did not reach statistical significance (p = 0.070). Reductions in serum 18:2n-6 by dairy consumption could have positive health implications but more studies are needed to confirm this assertion.


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